Alcohol-free compound hand disinfectant and preparation method thereof

Abstract

The invention discloses an alcohol-free compound hand disinfectant and a preparation method thereof, belonging to the technical field of disinfectants. The hand disinfectant comprises the following raw materials in percentage by weight: polyhexamethylene biguanide: 0.4-0.5%, quaternary ammonium salt: 0.18-0.3%, synergistic aid: 0.22-0.3%, moisturizing agent: 2.2%, plant skin care component: 1.4%, pH regulator: 0-0.5%, and the balance is water. The alcohol-free compound hand disinfectant has the following beneficial effects: polyhexamethylene biguanide has the characteristics of high antibacterial activity, quaternary ammonium salt can form an antibacterial protective film to continuously kill bacteria, and has no irritation or damage to the skin; the synergistic aid can adjust the long-acting antibacterial performance of the disinfectant.

Description

Technical Field

[0001] The invention belongs to the technical field of disinfectants, and more specifically relates to an alcohol-free compound hand disinfectant and a preparation method thereof. Background Art

[0002] Hand hygiene of medical staff is an important part of the infection prevention and control process. Appropriate hand disinfectant products are not only responsible for medical staff, but also a protection for patients. Currently, most hand disinfectants on the market contain alcohol substances, which have the advantages of quick drying and rapid sterilization. However, alcohol hand disinfectants have strong degreasing properties. Long-term and high-frequency use by medical staff can easily cause skin itching, allergies, etc., and there is a risk of hospital infection. The biosafety value of traditional alcohol-containing hand disinfectants is not high. The hand disinfectants on the market cannot have the effects of being non-irritating, less harmful, moisturizing and skin-protecting, and having continuous antibacterial effects.

[0003] Based on the above situation, an alcohol-free compound hand disinfectant with good bactericidal effect is developed, which has a sustained antibacterial effect and a high biosafety value, and has a good moisturizing and skin care effect on the hand skin. Summary of the invention

[0004] In order to solve the above problems and overcome the shortcomings of the prior art, the present invention provides an alcohol-free compound hand disinfectant and a preparation method thereof, which can effectively solve the problem that hand disinfectants cannot have the effects of being non-irritating, less harmful, moisturizing and skin-protecting, and having a continuous antibacterial effect.

[0005] The specific technical solution of the present invention to solve the above technical problems is as follows: the alcohol-free compound hand disinfectant of the present invention is composed of the following raw materials in percentage by weight:

[0006]

[0007] The polyhexamethylene biguanide is of medical grade, the quaternary ammonium salt is a mixture of decyldimethylammonium carbonate and didecyldimethylammonium formate, the moisturizer is at least one of glycerol and allantoin, the plant skin care ingredient is aloe extract, the synergistic aid is rhamnolipid, and the pH adjuster is one or more of urea, ethylamine, ethanolamine, triethylamine, triethanolamine, and butylamine.

[0008] The preparation method of the alcohol-free hand disinfectant is as follows: polyhexamethylene biguanide is added into a container, stirring is started, purified water is added, the rotation speed is 20-200r / min, quaternary ammonium salt, synergistic aid, moisturizer, plant skin care ingredients, pH adjuster, etc. are added in sequence, stirring for 40-60 minutes, and the hand disinfectant is obtained.

[0009] The hand disinfectant is a spray-type disinfectant, which is evenly dispersed when sprayed, does not contain any inducing odor, and the raw materials are mild, natural and non-irritating. Different from single guanidine disinfectants, the compounded quaternary ammonium salt component makes the finished product have a continuous antibacterial effect. The presence of the synergistic auxiliary agent can enhance the continuous antibacterial performance of the disinfectant by acting with the quaternary ammonium salt. The ratio of natural skin care factors and auxiliary agents can effectively moisturize the skin of the hands and avoid uncomfortable reactions.

[0010] The hand disinfectant can be used for daily hand hygiene and surgical hand disinfection.

[0011] The method of using the disinfectant is as follows: use it according to the standard method for disinfecting hygienic hands and surgical hands, it can effectively kill natural bacteria on the hands and has a sustained antibacterial effect, improves compliance with hand hygiene, and reduces hospital infection rates; the alcohol-free hand disinfectant has no alcohol odor, the ingredients are natural, mild, non-irritating, and have high biological safety.

[0012] The beneficial effects of the present invention are:

[0013] The present invention is different from the common alcohol hand disinfectants on the market. It does not contain alcohol. It uses guanidine disinfectants and quaternary ammonium salts as ingredients for synergistic sterilization. The system is stable and has high biosafety. Polyhexamethylene biguanide has the characteristics of high antibacterial activity. Quaternary ammonium salts can form an antibacterial protective film to continuously kill bacteria without irritating or damaging the skin. Synergistic additives can adjust the long-lasting antibacterial properties of the disinfectant. This product solves the problem of hand disinfectant products on the market being non-irritating, less harmful, refreshing and moisturizing, and having a continuous antibacterial effect. It has a high biosafety value and a high development prospect in the disinfection market. The hand disinfectant described in the present invention is easy to operate, has mild and non-irritating raw materials, has an outstanding bactericidal effect, and has a good feel and skin care performance.

[0014] The hand disinfectant prepared by the present invention utilizes the compound of quaternary ammonium salts of counter-ion structure, decyldimethylammonium carbonate and didecyldimethylammonium formate, to act with rhamnolipid. In the formula system, rhamnolipid can further change the structure of the molecule through the action with the quaternary ammonium salt molecules of the counter-ion structure, so as to achieve the characteristics of controllable long-term antibacterial effect, excellent bactericidal ability, continuous antibacterial effect and high biosafety, and can effectively reduce the probability of nosocomial infection. The synergist is non-toxic and 100% biodegradable. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Attached Figure 1 It is the plate diagram of the blank control, 3d and 7d continuous antibacterial test in Example 1 of the present invention;

[0016] Attached Figure 2 It is a plate diagram of the blank control, 3d and 7d continuous antibacterial test in Comparative Example 1 of the present invention;

[0017] Attached Figure 3It is a plate diagram of the blank control, 3d and 7d continuous antibacterial test in Comparative Example 2 of the present invention;

[0018] Attached Figure 4 It is a plate diagram of the blank control, 3d and 7d continuous antibacterial test in Comparative Example 3 of the present invention;

[0019] Attached Figure 5 It is a schematic diagram of the sites where the quaternary ammonium salt of the counter ion structure of the present invention affects the molecular structure of rhamnolipid. DETAILED DESCRIPTION

[0020] In the description of the present invention, specific details are only for a full understanding of the embodiments of the present invention, but those skilled in the art should know that the implementation of the present invention is not limited to these details. In addition, well-known structures and functions are not described or shown in detail to avoid blurring the key points of the embodiments of the present invention. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0021] Specific implementation of the present invention:

[0022] In order to better understand the present invention, a specific embodiment is used for illustration. It is worth emphasizing that the effect of this embodiment is not substantially different from the various embodiments within the protection scope of the present invention, including the respective reagents and the content ratio of the reagents, and all of them can achieve the effects described in the present invention and solve the above-mentioned problems. Other combinations are not described here.

[0023] The present invention will be further described below with reference to specific embodiments, but is not limited thereto.

[0024] Example 1

[0025] A non-alcohol compound hand disinfectant, the disinfectant comprising the following components: 0.5% polyhexamethylene biguanide, 0.12% decyldimethylammonium carbonate, 0.06% didecyldimethylammonium formate, 0.6% allantoin, 1.6% glycerol, 1.4% aloe extract, 0.26% rhamnolipid and deionized water. The disinfectant prepared in this proportion is recorded as sample 1.

[0026] Example 2

[0027] A non-alcohol compound hand disinfectant, comprising the following components: 0.4% polyhexamethylene biguanide, 0.10% decyldimethylammonium carbonate, 0.10% didecyldimethylammonium formate, 0.6% allantoin, 1.6% glycerol, 1.4% aloe extract, 0.22% rhamnolipid and deionized water. The disinfectant prepared in this proportion is recorded as sample 2.

[0028] Example 3

[0029] A non-alcohol compound hand disinfectant, comprising the following components: 0.5% polyhexamethylene biguanide, 0.06% decyldimethylammonium carbonate, 0.12% didecyldimethylammonium formate, 0.6% allantoin, 1.6% glycerol, 1.4% aloe extract, 0.25% rhamnolipid and deionized water. The disinfectant prepared in this proportion is recorded as sample 3.

[0030] Example 4

[0031] A non-alcohol compound hand disinfectant, the disinfectant comprising the following components: 0.4% polyhexamethylene biguanide, 0.03% decyldimethylammonium carbonate, 0.18% didecyldimethylammonium formate, 0.6% allantoin, 1.6% glycerol, 1.4% aloe extract, 0.30% rhamnolipid, 0.12% triethanolamine aqueous solution and deionized water. The disinfectant prepared in this proportion is recorded as sample 4.

[0032] Example 5

[0033] A non-alcohol compound hand disinfectant, the disinfectant comprising the following components: 0.4% polyhexamethylene biguanide, 0.15% decyldimethylammonium carbonate, 0.15% didecyldimethylammonium formate, 0.6% allantoin, 1.6% glycerol, 1.4% aloe extract, 0.30% rhamnolipid, 0.18% triethanolamine aqueous solution and deionized water. The disinfectant prepared in this proportion is recorded as sample 5.

[0034] Samples 1-5 were tested according to the Technical Specifications for Disinfection (2002 edition).

[0035] Microbial killing test: The suspension quantitative sterilization test method was used to test the sterilization performance. At 19-21°C, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, and Candida albicans were exposed to the bacteria for 1 minute, and the killing logarithm was detected. The killing logarithm of Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa was ≥5.0; the killing logarithm of Candida albicans was ≥4.0; after 1 minute of disinfection, the average killing logarithm of natural bacteria on the hand surface was ≥1.0, which met the qualified standards for hand disinfectant disinfection.

[0036] In order to more intuitively demonstrate the process advantages of the continuous antibacterial effect of the present invention, the present invention adopts a replacement method for comparison.

[0037] Comparative Example 1

[0038] An alcohol-free compound hand disinfectant comprises the following components: 0.5% polyhexamethylene biguanide, 0.12% decyl dimethyl ammonium carbonate, 0.06% didecyl dimethyl ammonium formate, 0.6% allantoin, 1.6% glycerol, 1.4% aloe extract and deionized water.

[0039] Comparative Example 2

[0040] An alcohol-free compound hand disinfectant comprises the following components: 0.5% polyhexamethylene biguanide, 0.6% allantoin, 1.6% glycerol, 1.4% aloe extract, 0.26% rhamnolipid and deionized water.

[0041] Comparative Example 3

[0042] An alcohol-free compound hand disinfectant comprises the following components: 0.5% polyhexamethylene biguanide, 0.18% benzalkonium chloride, 0.6% allantoin, 1.6% glycerol, 1.4% aloe extract, 0.26% rhamnolipid and deionized water.

[0043] Comparative Example 4

[0044] An alcohol-free compound hand disinfectant comprises the following components: 0.5% polyhexamethylene biguanide, 0.18% octyldecyl dimethyl ammonium chloride, 0.6% allantoin, 1.6% glycerol, 1.4% aloe extract, 0.26% rhamnolipid and deionized water.

[0045] Comparative Example 5

[0046] An alcohol-free compound hand disinfectant comprises the following components: 0.5% polyhexamethylene biguanide, 0.10% decyl dimethyl ammonium carbonate, 0.6% allantoin, 1.6% glycerol, 1.4% aloe extract, 0.26% rhamnolipid and deionized water.

[0047] Comparative Example 6

[0048] An alcohol-free compound hand disinfectant comprises the following components: 0.5% polyhexamethylene biguanide, 0.10% didecyldimethylammonium formate, 0.6% allantoin, 1.6% glycerol, 1.4% aloe extract, 0.26% rhamnolipid and deionized water.

[0049] Comparative Example 7

[0050] An alcohol-free compound hand disinfectant comprises the following components: 0.5% polyhexamethylene biguanide, 0.06% didecyldimethylammonium bromide, 0.06% didecyldimethylammonium chloride, 0.6% allantoin, 1.6% glycerol, 1.4% aloe extract, 0.26% rhamnolipid and deionized water.

[0051] Comparative Example 8

[0052] An alcohol-free compound hand disinfectant comprises the following components: 0.5% polyhexamethylene biguanide, 0.10% didecyldimethylammonium bromide, 0.6% allantoin, 1.6% glycerol, 1.4% aloe extract, 0.26% rhamnolipid and deionized water.

[0053] Comparative Example 9

[0054] An alcohol-free compound hand disinfectant comprises the following components: 0.5% polyhexamethylene biguanide, 0.10% didecyldimethylammonium chloride, 0.6% allantoin, 1.6% glycerol, 1.4% aloe extract, 0.26% rhamnolipid and deionized water.

[0055] Comparative Example 10

[0056] An alcohol-free compound hand disinfectant comprises the following components: 0.5% polyhexamethylene biguanide, 0.10% didecyldimethylammonium chloride, 0.6% allantoin, 1.6% glycerol, 1.4% aloe extract and deionized water.

[0057] Table 1: Composition table of different embodiments and comparative examples

[0058] ,

[0059] Examples 1-5 and Comparative Examples 1-10 were used to prepare alcohol-free compound hand disinfectants, and the sustained antibacterial and sustained antibacterial effects were determined. The determination method was based on WS / T 650 "Antibacterial and Antibacterial Effect Evaluation Method" to conduct sustained antibacterial effect tests. The results are detailed in Table 2;

[0060] Table 2: Continuous antibacterial test of different embodiments and comparative examples

[0061] ,

[0062] From the data in Table 2, we can see that:

[0063] (1) Examples 1-5 not only have positive effects in short-term bactericidal effect, but also have antibacterial effects in terms of sustained antibacterial and sustained antibacterial effects in accordance with the "Antibacterial and Antibacterial Effect Evaluation Method";

[0064] (2) Compared with Comparative Example 1, Example 1 differs in that the synergistic auxiliary agent, rhamnolipid, is not added, resulting in a certain degree of reduction in the sustained antibacterial effect, especially in the 7-day sustained antibacterial test, the effect is only 91.36%, which is quite different from the initial antibacterial effect. This shows that the sustained antibacterial performance of the alcohol-free compound hand disinfectant prepared in Comparative Example 1 is poor. Therefore, rhamnolipid has a certain promoting effect on the sustained antibacterial performance of specific quaternary ammonium salt molecules;

[0065] (3) Compared with Example 1 and Comparative Examples 2-4, the difference lies in the different ways of adding quaternary ammonium salts. Among them, Comparative Example 2 does not add quaternary ammonium salts, and Comparative Examples 3 and Comparative Examples 4 only add ordinary quaternary ammonium salts, resulting in extremely poor sustained antibacterial and sustained antibacterial effects. Since Rhamnolipids are added to Comparative Examples 2-4, they do not play a certain role in promoting sustained antibacterial performance. It can be seen that the promotion of Rhamnolipids on sustained antibacterial performance is closely related to the type and combination of quaternary ammonium salts.

[0066] (4) Compared with Comparative Examples 5-6, Example 1 differs in that the quaternary ammonium salt is added in a compounded manner or added alone, namely:

[0067] This group of experiments is to study the effect of compound quaternary ammonium salt on sustained antibacterial performance. According to the experimental data, it can be seen that in comparative examples 5-6, the 3-day sustained antibacterial test is not much different from that in Example 1, while in the 7-day sustained antibacterial test, the effect is only 92%, and the sustained antibacterial performance has the disadvantage of a high decline rate. It can be seen that the compound quaternary ammonium salt of decyl dimethyl ammonium carbonate and didecyl dimethyl ammonium formate has a significant effect on the 7-day sustained antibacterial test;

[0068] (5) Compared with Example 1 and Comparative Example 7, the difference is that the types of compounded quaternary ammonium salts are different.

[0069] Although Comparative Example 7 uses a composite quaternary ammonium salt, which is in the form of didecyl dimethyl ammonium bromide and didecyl dimethyl ammonium chloride, the 7-day sustained antibacterial test effect is even lower than that of didecyl dimethyl ammonium carbonate or didecyl dimethyl ammonium formate in Comparative Examples 5-6, which shows that not all composite quaternary ammonium salts can work together with rhamnolipid to promote sustained antibacterial performance to a certain extent;

[0070] (6) Compared with Example 1 and Comparative Examples 7, 8-9, the difference is that the addition methods of quaternary ammonium salts are different. From the experimental data, it can be seen that the 7-day sustained antibacterial test effect of Comparative Example 7 is not outstanding. Therefore, not all compounded quaternary ammonium salts have a certain promoting effect on the sustained antibacterial performance relative to the quaternary ammonium salts added alone;

[0071] (7) Compared with Comparative Examples 10 and 8-9, Example 1 differs in that the addition methods of quaternary ammonium salts are different, and the addition of rhamnolipids is also different.

[0072] As shown in Table 2, comparative example 10 has a certain improvement in the 7d continuous antibacterial test, but the antibacterial rate is still low, although rhamnolipid is not added, relative to comparative example 9. In view of this abnormal phenomenon, the inventors conducted a large number of experiments and inferred based on experimental data that the principle may be that the molecular structure of rhamnolipid has an amphiphilic group, and when rhamnolipid is compounded with decyl dimethyl ammonium carbonate and didecyl dimethyl ammonium formate in a certain proportion, the specific group action of rhamnolipid and the quaternary ammonium salt of the counterion structure will affect the charge density around the N atom; the amphiphilic structure affects the spatial arrangement of the quaternary ammonium salt molecules, increases the adsorption of the quaternary ammonium salt molecules and the bacteria, and thus has a synergistic effect on the long-acting antibacterial effect of the quaternary ammonium salt molecules of the specific counterion structure; when compounded with other types of quaternary ammonium salts, a spatial influence cannot be formed, and the presence of rhamnolipid instead leads to a decline in the long-acting antibacterial effect of the disinfectant.

[0073] In summary:

[0074] The present invention is different from the common alcohol hand disinfectants on the market. It does not contain alcohol. It uses guanidine disinfectants and quaternary ammonium salts as ingredients for synergistic sterilization. The system is stable and has high biosafety. Polyhexamethylene biguanide has the characteristics of high antibacterial activity. Quaternary ammonium salts can form an antibacterial protective film to continuously kill bacteria without irritating or damaging the skin. Synergistic additives can adjust the long-lasting antibacterial properties of the disinfectant. This product solves the problem of hand disinfectant products on the market being non-irritating, less harmful, refreshing and moisturizing, and having a continuous antibacterial effect. It has a high biosafety value and a high development prospect in the disinfection market. The hand disinfectant described in the present invention is easy to operate, has mild and non-irritating raw materials, has an outstanding bactericidal effect, and has a good feel and skin care performance.

[0075] The alcohol hand disinfectant prepared by the invention utilizes the compound of quaternary ammonium salts decyl dimethyl ammonium carbonate and didecyl dimethyl ammonium formate to react with rhamnolipid to achieve the characteristic of controllable long-term antibacterial effect.

[0076] The alcohol hand disinfectant prepared by the present invention utilizes the compound of quaternary ammonium salts of counter-ion structure, decyldimethylammonium carbonate and didecyldimethylammonium formate, to act with rhamnolipid. In the formula system, rhamnolipid can further change the structure of the molecule through the action with the quaternary ammonium salt molecules of the counter-ion structure, so as to achieve the characteristics of controllable long-term antibacterial effect, excellent bactericidal ability, continuous antibacterial effect and high biosafety, and can effectively reduce the probability of nosocomial infection. The synergist is non-toxic and 100% biodegradable.

Claims

1. An alcohol-free compound hand disinfectant, characterized in that The invention is composed of the following raw materials in percentage by weight: polyhexamethylene biguanide: 0.4-0.5%, quaternary ammonium salt: 0.18-0.3%, synergist: 0.22-0.3%, moisturizer: 2.2%, plant skin care ingredient: 1.4%, pH adjuster: 0-0.5%, and the balance is water; The quaternary ammonium salt is a mixture of decyl dimethyl ammonium carbonate and didecyl dimethyl ammonium formate; the mass ratio of decyl dimethyl ammonium carbonate to didecyl dimethyl ammonium formate is (2-1): (1-6); The synergistic aid is rhamnolipid; The moisturizing agent is at least one of glycerin and allantoin; The moisturizing agent is a plant skin care ingredient and is an aloe extract.

2. The alcohol-free compound hand disinfectant according to claim 1, characterized in that The pH regulator is one or more of urea, ethylamine, ethanolamine, triethylamine, triethanolamine, and butylamine.

3. A method for preparing an alcohol-free compound hand disinfectant, using the alcohol-free compound hand disinfectant as claimed in claim 1 or 2, characterized in that The preparation method comprises the following steps: adding polyhexamethylene biguanide into a container, starting stirring, adding purified water, rotating at a speed of 20-200 r / min, sequentially adding quaternary ammonium salt, synergistic aid, moisturizer, plant skin care ingredients, pH adjuster, etc., stirring for 40-60 minutes, and obtaining the alcohol-free compound hand disinfectant.

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