Method for improving germination rate of banana seeds and application thereof

Abstract

The present application relates to the technical field of banana seed germination, and particularly relates to a method for improving the germination rate of banana seeds and application thereof. The method for improving the germination rate of banana seeds comprises the following steps: treating banana seeds with ultrasonic waves and then treating the banana seeds with a seed soaking solution. The seed soaking solution is a mixed solution of AIDOCO and gibberellin. The present application can effectively promote the rapid and healthy germination of banana seeds, increase the germination rate of seeds, help obtain banana varieties with high disease resistance and high quality, and improve the planting benefits of farmers.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of banana seed germination, in particular to a method for improving the germination rate of banana seeds and application thereof. BACKGROUND

[0002] Banana (Musa nana Lour.) is a perennial herbaceous plant of Musaceae family, with clonal stems and rhizomatous stems; the false stem is dark green with black spots; the leaf blade is long and circular, dark green on the upper surface, and rarely powdery for Musa acuminate and Musa sapientum, and powdery for Musa paradisiaca; the flower is milky white or light purple; the fruit is curved and arched, or straight; the fruit skin is green, and turns yellow after ripening; the fruit pulp is soft and yellowish white, sweet and fragrant; and the wild banana varieties have seeds.

[0003] As one of the important fruit crops, banana has a single variety, especially a lack of disease-resistant varieties, which requires the creation of new disease-resistant varieties. Most of the existing banana cultivars are triploid, the pollen of male flowers lacks vitality, the female flowers are highly sterile, the male flowers of some diploid cultivars are fertile, and the female flowers are also highly sterile, and only wild diploid has good fertility. The cross between triploid and diploid is limited to certain varieties, and only a small amount of seeds can be obtained; on the other hand, the germination rate of hybrid banana seeds is low, and the seeds almost do not germinate under natural conditions, which greatly limits the process of new variety breeding by hybridization breeding. Although hybridization breeding is an important way to create new varieties, the germination rate of hybrid seeds is low, only 0.3% to 3.0%, which seriously limits the efficiency and process of hybridization breeding. Therefore, it is of great significance to find an effective method to improve the germination rate of banana seeds. SUMMARY

[0004] The present application aims to provide a method for improving the germination rate of banana seeds and application thereof, which improves the germination rate of hybrid banana seeds by treating the seeds with ultrasound and drugs.

[0005] In order to achieve the above-mentioned application purposes, the present application provides the following technical solutions:

[0006] The present application provides a method for improving the germination rate of banana seeds, which uses ultrasonic treatment on banana seeds and then uses seed soaking solution for seed soaking; the seed soaking solution is a mixed solution of Amdox and gibberellin.

[0007] Preferably, the mass ratio of Amdox to gibberellin is 7-9:100-200.

[0008] Preferably, the final concentration of Amdox in the seed soaking solution is 7-9 mg / L.

[0009] Preferably, the final concentration of gibberellin in the seed soaking solution is 100-200 mg / L.

[0010] Preferably, the AIDUCO is 1.8% AIDUCO water agent.

[0011] Preferably, the power of the ultrasonic is 15-25 W / L; the time of the ultrasonic is 4-6 min.

[0012] Preferably, the variety of the banana seed is Lengajiao (South China wild banana). Its scientific name is Musa balbisiana Colla, alias Lengajiao, mountain banana, pull banana, the pulp has a long handle, the handle is more than 2 cm long; the male flower is dark purple or purple; the seed is oblate; the domestic main distribution is in the west of Yunnan, Guangxi, Guangdong, and is in the humid evergreen forest of valley slope; the foreign main distribution is in the south and southeast of Asia. The resource is collected in Guangdong, and we name it as Lengajiao.

[0013] Preferably, the time of the seed soaking is 16-24 h.

[0014] The application further provides application of the method in improving the germination rate of banana seeds.

[0015] Compared with the prior art, the application has the beneficial effects as follows:

[0016] The banana seeds are first treated by ultrasonic waves in the application, which can corrode the seed coat without damaging the embryo, and can physically destroy the hard banana seed coat.

[0017] The main effective component of AIDUCO in the seed soaking liquid used in the application is sodium complex nitrophenol, which is a strong cell activator, and the chemical components are 5-nitrosodium guaiacol, sodium o-nitrophenol and sodium p-nitrophenol. After contacting with the banana seeds, it can rapidly penetrate into the seed interior, promote the protoplasm flow of cells, improve the cell activity, break the dormancy, promote the germination of banana seeds and accelerate the growth speed of banana. The gibberellin can promote the decomposition of starch in the seed, and plays a regulating role in seed germination. The two are used in combination, which can promote the decomposition of the substances stored in the banana seed interior, and accelerate the germination of banana seeds.

[0018] The method of the application can effectively promote the rapid and healthy germination of banana seeds, increase the seed germination rate and help to obtain banana varieties with strong disease resistance and high quality, and improve the planting benefits of farmers. BRIEF DESCRIPTION OF DRAWINGS

[0019] In order to more clearly illustrate the technical solutions in the embodiments of the application or the prior art, the following will briefly introduce the drawings needed to be used in the embodiment or prior art description. Obviously, the drawings in the following description are only the embodiments of the application, and other drawings can be obtained by those skilled in the art without creative labor on the basis of the provided drawings.

[0020] Figure 1 Figure for the germination test of banana seeds of Example 1.

[0021] Figure 2 Figure for the germination test of banana seeds of Example 2.

[0022] Figure 3 Figure for the germination test of banana seeds of Example 3. DETAILED DESCRIPTION

[0023] The application provides a method for improving the germination rate of banana seeds, which comprises the steps of treating the banana seeds with ultrasonic waves and then soaking the seeds in a soaking solution.

[0024] In the application, the mass ratio of the aprotic solvent and the gibberellin is 7-9:100-200; preferably 7-9:120-180; further preferably 8:140-160; and more preferably 8:150.

[0025] In the application, the final concentration of the aprotic solvent in the soaking solution is 7-9 mg / L; and preferably 8 mg / L.

[0026] In the application, the final concentration of the gibberellin in the soaking solution is 100-200 mg / L; preferably 120-180 mg / L; further preferably 140-160 mg / L; and more preferably 150 mg / L.

[0027] In the application, the aprotic solvent is 1.8% aprotic solvent water agent.

[0028] In the application, the power of the ultrasonic waves is 15-25 W / L; preferably 17-23 W / L; further preferably 19-21 W / L; and more preferably 20 W / L.

[0029] In the application, the time of the ultrasonic waves is 4-6 min; and preferably 5 min.

[0030] In the application, the variety of the banana seeds is Leng Aixiao.

[0031] In the application, the time of the soaking is 16-24 h; and preferably 20 h.

[0032] The application further provides the use of the method in improving the germination rate of banana seeds.

[0033] The technical solutions provided by the application will be described in detail below in combination with the examples, but they should not be understood as limiting the protection scope of the application.

[0034] In the present application, gibberellins are purchased from Shandong Lu Kang Biological Pesticide Co., Ltd.; abscisic acid is purchased from Xuzhou Chemical Industry (Zhangzhou) Co., Ltd. In the present application, the soaking time of abscisic acid and / or gibberellins, and water treatment experiments is 20 h.

[0035] Experimental Example 1

[0036] The banana seed variety is Leng Aijiao. The collected fresh banana seeds are divided into several groups. Specifically:

[0037] 1.8% abscisic acid (concentration 3.0 mg / L) + gibberellins (concentration 100 mg / L), set as group A;

[0038] 1.8% abscisic acid (concentration 3.0 mg / L) + gibberellins (concentration 200 mg / L), set as group B;

[0039] 1.8% abscisic acid (concentration 3.0 mg / L) + gibberellins (concentration 300 mg / L), set as group C;

[0040] 1.8% abscisic acid (concentration 5.0 mg / L) + gibberellins (concentration 100 mg / L), set as group D;

[0041] 1.8% abscisic acid (concentration 5.0 mg / L) + gibberellins (concentration 200 mg / L), set as group E;

[0042] 1.8% abscisic acid (concentration 5.0 mg / L) + gibberellins (concentration 300 mg / L), set as group F;

[0043] 1.8% abscisic acid (concentration 7.0 mg / L) + gibberellins (concentration 100 mg / L), set as group G;

[0044] 1.8% abscisic acid (concentration 7.0 mg / L) + gibberellins (concentration 200 mg / L), set as group H;

[0045] 1.8% abscisic acid (concentration 7.0 mg / L) + gibberellins (concentration 300 mg / L), set as group I;

[0046] 1.8% abscisic acid (concentration 9.0 mg / L) + gibberellins (concentration 100 mg / L), set as group J;

[0047] 1.8% abscisic acid (concentration 9.0 mg / L) + gibberellins (concentration 200 mg / L), set as group K;

[0048] 1.8% abscisic acid (concentration 9.0 mg / L) + gibberellins (concentration 300 mg / L), set as group L;

[0049] Physical destruction of seed umbilical cord, set as group M;

[0050] The control group is soaked in water for 20 hours, and is set as CK.

[0051] The soaking time of AIDUCO and gibberellin used alone or in combination is 20 hours.

[0052] Each test group handles 150 banana seeds, and after the completion of each test group, it is sowed in peat soil medium in a glass greenhouse. The specific method is:

[0053] 1. Draw a line, dig a ditch: seedbed treatment, land preparation, remove debris and stones in the layer.

[0054] 2. Sowing: evenly spread banana seeds on the bed surface.

[0055] 3. Covering: after sowing, cover a layer of sieved sheep manure, 2 cm thick.

[0056] 4. Pressing: in the case of dry weather and loose soil, pressing is required after covering, but in clay areas or when the soil is too wet, pressing is not recommended, as it may cause soil compaction and hinder seedling emergence.

[0057] 5. Post-sowing management: temperature and humidity management should be done well, and the temperature should not be lower than 12℃. During the seedling emergence period, water should be supplemented by uncovering the membrane, but excessive irrigation should be avoided to affect the soil temperature; if the water content is too high, the membrane should be uncovered to dissipate the moisture, so as to prevent the lack of oxygen and low temperature from causing uneven emergence. The air temperature should be maintained above 10℃, and the soil temperature should be maintained at 12-38℃.

[0058] During the period (from July 20 to August 2), the germination of each group of seeds was observed, and the results are shown in Table 1.

[0059] Table 1 Banana seed germination rate

[0060]

[0061]

[0062] The results show that the germination rate of banana seeds in groups G and K is significantly higher than that in other test groups. 1.8% AIDUCO (concentration 7.0 mg / L) + gibberellin (concentration 100 mg / L); 1.8% AIDUCO (concentration 9.0 mg / L) + gibberellin (concentration 200 mg / L) can help break the dormancy of banana seeds and improve their germination rate. Figure 1 The CK group has some death of banana buds due to pests and diseases.

[0063] Experimental Example 2

[0064] The banana seed variety is Leng Aixiao. The collected fresh banana seeds are divided into several groups. Specifically:

[0065] 1. Gibberellin (concentration 100 mg / L); Group A

[0066] 2. Gibberellin (concentration 200 mg / L); Group B

[0067] 3. Gibberellin (concentration 300 mg / L); Group C

[0068] 4. 1.8% Ethrel (concentration 3.0 mg / L); Group D

[0069] 5. 1.8% Ethrel (concentration 5.0 mg / L); Group E

[0070] 6. 1.8% Ethrel (concentration 7.0 mg / L); Group F

[0071] 7. 1.8% Ethrel (concentration 9.0 mg / L); Group G

[0072] 8. 1.8% Ethrel (concentration 7.0 mg / L) + Gibberellin (concentration 100 mg / L); Group H

[0073] 9. 1.8% Ethrel (concentration 9.0 mg / L) + Gibberellin (concentration 200 mg / L); Group I

[0074] 10. 98% concentrated sulfuric acid 10 s soak; Group J

[0075] 11. 98% concentrated sulfuric acid 20 s soak; Group K

[0076] 12. 98% concentrated sulfuric acid 30 s soak; Group L

[0077] 13. 98% concentrated sulfuric acid 40 s soak; Group M

[0078] 14. 98% concentrated sulfuric acid 50 s soak; Group N

[0079] 15. 98% concentrated sulfuric acid 60 s soak; Group O

[0080] 16. Rubbing seeds with coarse river sand; Group P

[0081] 17. Ultrasonic treatment (20 W / L) of seeds for 5 min; Group Q

[0082] 18. Rubbing seeds with coarse river sand, then ultrasonic treatment (20 W / L) for 5 min; Group R

[0083] 19. 98% concentrated sulfuric acid 20 s soak, then rubbing seeds with coarse river sand (all the pulp and other impurities on the surface of the seeds are rubbed off until the water is no longer colored), and finally ultrasonic treatment (20 W / L) for 5 min; Group S

[0084] The soaking time of the seed with Acdosol and gibberellin alone or in combination is 20 hours.

[0085] Each test group was treated with 1021 banana seeds, 10-20 groups (J-T), and sowed on August 9, 2022. Groups 1-9 (A-I) were sowed on August 10, 2022. The specific planting method is as follows:

[0086] 1. Cleaning and selection of plug trays: clean and disinfect the plug trays.

[0087] 2. Selection and correct configuration of the substrate: mix peat soil and vermiculite at a ratio of 2:1 as the substrate, and use it after sieving.

[0088] 3. Substrate filling: fill the substrate into the plug trays before sowing, scrape off the excess substrate, and avoid compacting it by force. Then, gently press the plug trays filled with the substrate with fingers.

[0089] 4. Sowing: carefully point the seeds into the plug trays, one seed per hole.

[0090] 5. Covering the substrate: evenly cover it with the substrate.

[0091] 6. Post-sowing management: spray water after sowing, and spray until water seeps out from the bottom of the plug trays.

[0092] 7. Seedling emergence: remove the mulch film in time after the seedlings emerge, and immediately perform thinning and transplanting to make up for the lack.

[0093] 8. Timely check the state of the plug tray substrate, and supplement water when the surface turns white.

[0094] 9. Add a heating device in winter to keep the room temperature above 12℃, and add a cooling device in summer to keep the room temperature below 30℃.

[0095] Observe the germination of the seeds in each group (on August 23 and August 25), and the results are shown in Table 2.

[0096] Table 2 Germination rate of banana seeds

[0097]

[0098] The results show that the four treatments with the highest germination rate are: ① 1.8% Acdosol (concentration 7.0 mg / L) + gibberellin (concentration 100 mg / L); ② 1.8% Acdosol (concentration 9.0 mg / L) + gibberellin (concentration 200 mg / L); ③ ultrasonic treatment for 5 minutes; and ④ rubbing the seeds with coarse sand + ultrasonic treatment for 5 minutes. It is speculated that the use of 1.8% Acdosol (concentration 7.0-9 mg / L) + gibberellin (concentration 100-200 mg / L) after ultrasonic treatment can effectively improve the seed germination rate. Figure 2 ​

[0099] Experimental Example 3

[0100] The comparative test is specifically grouped as follows:

[0101] 1. Water soaking for 20h as a control group (CK);

[0102] 2. Group A: 1.8% AIDUCO (concentration 7.0mg / L) + gibberellin (concentration 100mg / L);

[0103] 3. Group B: ultrasonic wave (20W / L) for 5min;

[0104] 4. Group C: ultrasonic wave (20W / L) for 5min, then 1.8% AIDUCO (concentration 7.0mg / L) + gibberellin (concentration 100mg / L) treatment.

[0105] The soaking time of AIDUCO and gibberellin combination is 20h.

[0106] 4000 fresh and full Lengai seeds with large density (sink to the bottom of water) were selected. 1000 seeds were randomly selected in each group. The seeds were sowed on June 1, 2024 (the sowing method was the same as that in Example 2), and germination started on June 9, 2022. The germination counting time was July 7, 2024. The germination number and germination rate of different experimental groups were counted, and the results are shown in Table 3.

[0107] Table 3 Germination rate statistics

[0108] Group Total number of germination Germination rate (%) CK 98 9.8 A 214 21.4 B 159 15.9 C 223 22.3

[0109] The results show that the highest germination rate is group C, which can reach 22.3%, followed by group A and group B, which shows that ultrasonic treatment of banana seeds can help seed germination. This also shows that the ultrasonic + AIDUCO and gibberellin combination treatment method can significantly improve the germination rate of banana seeds, and is better than other treatment methods of the prior art.

[0110] The above only describes the preferred embodiments of the present application. It should be noted that for ordinary skilled persons in the art, several improvements and refinements can be made without departing from the principles of the present application, and these improvements and refinements should also be considered within the protection scope of the present application.

Claims

1. A method of increasing the germination rate of banana seeds, characterized by, The banana seeds are treated by ultrasonic wave and then soaked in a soaking solution which is a mixture of abscisic acid and gibberellin; The mass ratio of abscisic acid to gibberellin is 7-9:100-200; The final concentration of abscisic acid in the soaking solution is 7-9 mg / L; The final concentration of gibberellin in the soaking solution is 100-200 mg / L; The abscisic acid is 1.8% abscisic acid water agent; The power of ultrasonic wave is 15-25 W / L; the time of ultrasonic wave is 4-6 min.

2. The method of claim 1, wherein, The banana seeds are of the variety of Leng Ajiao.

3. The method of claim 1, wherein, The time of soaking is 16-24 h.

4. The use of the method of any one of claims 1-3 in improving the germination rate of banana seeds.

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