Application of 2-heptanone as an attractant in controlling rice leafhopper pests

Abstract

The present invention provides the use of 2-heptanone as an attractant for controlling rice leafhopper pests. Specifically, the rice volatile 2-heptanone is used as an attractant for controlling three types of leafhopper pests. The rice leafhopper pests include the black-tailed leafhopper, the electric leafhopper, or the two-spotted black-tailed leafhopper. The present invention finds that 2-heptanone is attractive to all three rice leafhopper pests, and can be used as a broad-spectrum attractant for pest control.

Description

Technical Field

[0001] The invention belongs to the technical field of crop disease and insect pest control, and particularly relates to the application of 2-heptanone as an attractant in controlling rice leafhopper pests. Background Art

[0002] Rice is an important food crop and plays a crucial role in the food supply. Rice is often harmed by leafhoppers. Leafhoppers (Blacktail, Electric, and Two-spotted) are common pests of rice and belong to the family Cicadidae in the order Hemiptera. These three leafhoppers not only directly feed on rice, causing damage, but also indirectly harm it by transmitting plant viruses. These direct and indirect damages hinder the high and stable yields of rice in my country.

[0003] The prevention and control of insect-borne viral diseases is extremely difficult due to the lack of highly effective virus-resistant germplasm resources and the small size and hidden nature of the vector insects. Currently, production primarily relies on chemical agents to control vector insects to curb viral outbreaks. However, excessive use of chemical agents has led to numerous problems. Therefore, new methods and approaches to control vector insects are urgently needed to ensure national food security.

[0004] Plant volatiles play an important role in a series of behaviors of herbivorous insects. Many herbivorous insects use plant volatiles to locate hosts, feed, mate and lay eggs. Therefore, attractants made from host plant volatiles have the ability to trap leafhopper pests and are an efficient and green method to prevent and control leafhopper pests and the plant viruses they spread. Summary of the Invention

[0005] The present invention aims to provide an application of 2-heptanone as an attractant in preventing and controlling rice leafhopper pests, that is, using the rice volatile 2-heptanone as an attractant for preventing and controlling three types of leafhopper pests.

[0006] The present invention first provides a new use of 2-heptanone, which is the use of 2-heptanone as an attractant in preventing and controlling rice leafhopper pests;

[0007] The rice leafhopper pests include black-tailed leafhopper, electric leafhopper or two-spotted black-tailed leafhopper.

[0008] The present invention also provides another use of 2-heptanone, which is the use in preparing an attractant for rice leafhopper pests;

[0009] In another aspect, the present invention provides a rice leafhopper pest attractant, wherein the attractant contains 2-heptanone at a pharmacologically effective concentration.

[0010] The pharmacologically effective concentration, as a specific record of the embodiment, is not less than 0.001 μg / μL.

[0011] The present invention finds that 2-heptanone is attractive to rice leafhopper pests such as the black-tailed leafhopper, the electric leafhopper or the two-spotted black-tailed leafhopper, and can be made into a broad-spectrum attractant for preventing and controlling leafhopper pests. The other three leafhopper pests can spread rice dwarf virus disease, rice gall dwarf virus disease and rice yellow dwarf virus disease. The attractant made from 2-heptanol can control the number of the three vector insects to reduce or prevent the spread of the virus disease, thereby achieving high and stable rice yield. BRIEF DESCRIPTION OF THE DRAWINGS

[0012] Figure 1 : Schematic diagram of the olfactory behavior measurement device;

[0013] Figure 2 :Data graph showing the effect of 2-heptanone on the behavior of black-tailed leafhopper;

[0014] Figure 3 :Data graph showing the effect of 2-heptanone on the behavior of electric leafhoppers;

[0015] Figure 4 :Data graph of the effects of 2-heptanone on the behavior of two-spotted black-tailed leafhopper. DETAILED DESCRIPTION

[0016] The black-tailed leafhopper and electric leafhopper used in the embodiment of the present invention were collected from the rice fields of the Agricultural Experimental Base of Zhejiang University in Changxing City, Zhejiang Province, and the two-spotted black-tailed leafhopper was collected from the rice fields of Xundian Hui and Yi Autonomous County, Kunming City, Yunnan Province. The collected insects were raised for multiple generations in the laboratory under the following conditions: temperature 25°C ± 1°C, photoperiod (light: dark) 14h: 10h, light intensity 3500-4000Lux, and relative humidity 60±5%. The susceptible rice variety (Taichung Native1, TN1) was selected for feeding three types of leafhoppers (Chang XF, Wang F, Fang Q, Chen F, Yao HW, Gatehouse AMR, Ye GY* (2021). Virus-induced plant volatiles mediate the olfactory behavior of its insect vectors. Plant Cell & Environment 44: 2700-2715). After three generations of continuous breeding, the newly emerged female adults were selected for olfactory behavior tests. The olfactory device was based on a previously reported trap device (Bi YL, Zhang X, Chang XF, Li X, Xiao S, Zhang B, Dang C, Sun LL, Yao HW, Fang Q, Wang F*, Ye GY* (2023). Olfactory behavioral responses of two Drosophila species and their pupal parasitoid to volatiles from bananas. Pest Management Science, 79: 4309-4318).

[0017] The 2-heptanone used in the present invention was purchased from the official website of Sigma-Aldrich, and the chromatographic grade standard and solvent liquid paraffin oil were purchased from Shanghai Sangon Bioengineering Technology Service Co., Ltd.

[0018] The present invention is described in detail below with reference to the embodiments and accompanying drawings.

[0019] Example 1: Behavioral response of black-tailed leafhopper to the rice volatile 2-heptanone

[0020] use Figure 1This device measures the behavioral responses of black-tailed leafhoppers to the rice volatile 2-heptanone. Standard 2-heptanone was prepared at five concentrations (0.001, 0.01, 0.1, 1, and 10 μg / μL) (dissolved in paraffin oil) to analyze their effects on the olfactory behavior of the black-tailed leafhoppers, from low to high. Using 0.001 μg / μL of 2-heptanone as an example, 5g of cotton wool was placed at each end of the device. One end dripped 500 μL of 0.001 μg / μL 2-heptanone onto the cotton wool as the test group, while the other end dripped 500 μL of paraffin oil onto the cotton wool as the control group. Fifteen female black-tailed leafhoppers, moisturized and starved for 4 hours, were placed in each cage. 24 hours later, the number of leafhoppers choosing the standard solution and paraffin oil control groups was recorded. The experiment was conducted in a climate chamber with humidity maintained throughout, at 27°C ± 1°C and a relative humidity of 75 ± 5%. Ten replicates were performed.

[0021] The experimental method and analysis method for the effect of 2-heptanone on the olfactory behavior of black-tailed leafhopper at concentrations of 0.01μg / μL, 0.1μg / μL, 1μg / μL, and 10μg / μL are the same as above.

[0022] Data were analyzed using SASS software, and the Student's test was used to analyze the significance of the differences between the treatment and control groups.

[0023] The behavioral responses of the black-tailed leafhopper to 2-heptanone are as follows Figure 2 The results showed that five concentrations of 2-heptanone were attractive to black-tailed leafhoppers.

[0024] Example 2: Behavioral response of the two-spotted black leafhopper to the rice volatile 2-heptanone

[0025] use Figure 1 This device measures the behavioral responses of the two-spotted black leafhopper to 2-heptanone in rice. Standard 2-heptanone was prepared at five concentrations (0.001, 0.01, 0.1, 1, and 10 μg / μL) (dissolved in paraffin oil) to analyze its effects on the olfactory behavior of the two-spotted black leafhopper. Using 0.001 μg / μL of 2-heptanone as an example, 5g of cotton wool was placed at each end of the device. One end dripped 500 μL of 0.001 μg / μL 2-heptanone onto the cotton wool as the test group, while the other end dripped 500 μL of paraffin oil onto the cotton wool as the control group. Fifteen adult female electric leafhoppers, moisturized and starved for 4 hours, were placed in each cage. After 24 hours, the number of leafhoppers choosing the standard solution and the paraffin oil control groups was recorded. The experiment was conducted in a climate chamber with humidity maintained throughout, at 27°C ± 1°C and a relative humidity of 75 ± 5%. Set 10 repetitions.

[0026] The experimental method and analysis method for the effect of 2-heptanone on the olfactory behavior of two-spotted black-tailed leafhopper at concentrations of 0.01μg / μL, 0.1μg / μL, 1μg / μL, and 10μg / μL are the same as above.

[0027] Data were analyzed using SASS software, and the Student's test was used to analyze the significance of the differences between the treatment and control groups.

[0028] The behavioral responses of the two-spotted black-tailed leafhopper to 2-heptanone are as follows: Figure 3 All five concentrations have an attractive effect, and as the concentration decreases, the attractive force tends to decrease slightly.

[0029] Example 3: Behavioral responses of electric leafhoppers to the rice volatile 2-heptanone

[0030] use Figure 1 This device measures the behavioral responses of electric leafhoppers to the rice volatile 2-heptanone. Standard 2-heptanone was prepared at five concentrations (0.001, 0.01, 0.1, 1, and 10 μg / μL) (dissolved in paraffin oil) to analyze their effects on the olfactory behavior of electric leafhoppers, from low to high. Using 0.001 μg / μL of 2-heptanone as an example, 5g of cotton wool was placed at each end of the device. One end dripped 500 μL of 0.001 μg / μL 2-heptanone onto the cotton wool as the test group, while the other end dripped 500 μL of paraffin oil onto the cotton wool as the control group. Fifteen female electric leafhoppers, moisturized and starved for 4 hours, were placed in each cage. 24 hours later, the number of leafhoppers choosing the standard solution and paraffin oil control groups was recorded. The experiment was conducted in a climate chamber with moisture retention throughout, at 27°C ± 1°C and 75 ± 5% relative humidity. Ten replicates were performed.

[0031] The experimental and analytical methods for the effects of 2-heptanone at concentrations of 0.01 μg / μL, 0.1 μg / μL, 1 μg / μL, and 10 μg / μL on electro-optical olfactory behavior were the same as above. Data were analyzed using SASS software, and the Student's test was used to analyze the significance of the differences between the treatment and control groups.

[0032] The results showed that the behavioral response of the electric leafhopper to 2-heptanone ( Figure 4 ), all five concentrations have an attractive effect.

[0033] In summary, it was found that 2-heptanone is attractive to the three major pests of rice leafhoppers and can be made into a broad-spectrum attractant for pest control.

Claims

1. Use of 2-heptanone as an attractant in controlling rice leafhopper pests, wherein the rice leafhopper pests are black-tailed leafhopper, electric leafhopper or two-spotted black-tailed leafhopper; The effective concentration of the 2-heptanone is 0.001 μg / μL-10 μg / μL.

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