A pesticide composition for preventing and controlling pine wood nematode disease and its application

Abstract

The present invention belongs to the technical field of pesticides and provides a pesticide composition for controlling pine wood nematode disease and its use. The composition comprises active ingredients A and B, wherein active ingredient A is selected from cyproflumizone; active ingredient B is selected from flupyridam; and the mass ratio of active ingredient A to active ingredient B is 1:5 to 4:1. By combining the insecticide cyproflumizone with the fungicide flupyridam, the present invention can achieve a superimposed or synergistic effect, effectively improving the rapid effectiveness of controlling pine wood nematodes. Simultaneously, the pine beetle, a carrier of the nematodes, is killed, ensuring the long-term effectiveness against pine wood nematodes and preventing the re-reproduction of eggs. The composition also has a preventive effect on pine wood and ensures the healthy growth of pine plants. The use of a polymethoxysilane adjuvant ensures the penetration of the composition, improves the conduction of the composition within the plant, and promotes the insecticidal effect of the combination of cyproflumizone and flupyridam.

Description

Technical Field

[0001] The present invention relates to the technical field of pesticides, in particular to a pesticide composition for preventing and treating pine wood nematode disease and application thereof. Background Art

[0002] The rapid spread of pine wilt nematode (Bursaphelenchus xylophilus) has already caused the death and withering of large numbers of pine trees, devastating pine forests in some areas and causing ecological disasters. In some scenic areas, valuable and ancient pines have been wiped out due to pine wilt disease, causing irreparable damage. Controlling the pine beetle, the insect vector that transmits the disease, remains the current method for preventing and controlling pine wilt disease. However, these methods are often ineffective or difficult to apply to tall trees in mountainous areas, resulting in limited success. Using a single injection of pesticide at the base of pine trees to prevent pine wilt disease is a necessary breakthrough for protecting pine forests.

[0003] However, the effect of using conventional pesticides to "inject" the base of pine trees is not ideal, and the prevention and control effect is relatively poor. The speed of effect and the duration of effectiveness of conventional pesticides are relatively poor, and the prevention and control effect cannot be achieved. Therefore, it is a technical problem that urgently needs to be solved. Summary of the Invention

[0004] The purpose of the present invention is to overcome the above-mentioned shortcomings of the prior art and provide a pesticide composition for controlling pine wood nematode disease, which has outstanding fast-acting properties, a long lasting effect, is easy to use, can well protect the normal growth and development of pine wood, and prevent pine wood nematodes from damaging pine trees, and its application.

[0005] The technical solution of the present invention is: a pesticide composition for preventing and controlling pine wood nematode disease, comprising active ingredients A and B, wherein the active ingredient A is selected from cyprodinil; the active ingredient B is selected from triflumuron; and the mass ratio of the active ingredient A to the active ingredient B is 1:5 to 4:1.

[0006] The advantage of this solution is that the insecticide ciprofloxacin is combined with the fungicide triflupyramide to control pine wood nematodes, which effectively improves the rapidity and duration of control of pine wood nematodes, reduces the cost of pine wood nematode control, and ensures the healthy growth of pine plants. The combination of the two has a superimposed or synergistic promoting effect on the control of pine wood nematodes. The composition of the present invention can also prevent pine wood nematodes and avoid pine wood nematode infection and damage. In addition, the insecticide ciprofloxacin can also effectively control longhorn beetles and prevent longhorn beetles from directly damaging pine trees. At the same time, it can also reduce the damage to pine wood caused by the spread of pine wood nematodes carried by longhorn beetles.

[0007] Furthermore, the mass ratio of the active ingredient A to the active ingredient B is 1:4 to 5:2.

[0008] Furthermore, the composition further comprises an auxiliary agent C and auxiliary materials, wherein the auxiliary agent C is selected from polymethoxysilane. Preferably, the use of polymethoxysilane can ensure that the particle size of the composition after dilution in water is less than 0.1 micron, thereby improving the conduction speed and dispersion effect of the composition within the plant. The smaller the particle size, the better the dispersion effect within the plant. In addition, the control effect on pine wood nematodes is also improved, and the composition is more easily absorbed into the body of pine wood nematodes, thereby improving the control effect on pine wood nematodes and increasing the transmission efficiency within the pine wood plant, thereby improving the rapid control effect on pine wood nematodes.

[0009] According to the present invention, the term "excipient" refers to an organic solvent that can be combined or associated with the active ingredient to make it easier to apply to agriculturally acceptable, accepted in pine trees and capable of conducting liquids: applicable liquid carriers can be selected from the following materials, such as water, alcohols (such as methanol, ethanol, isopropanol, butanol, ethylene glycol, isomeric alcohols, etc.), ketones (such as acetone, methyl ethyl ketone, diisobutyl ketone, cyclohexanone, etc.), amides (dimethylacetamide, dimethylcaprylyl, etc.), ethers and other water-miscible organic solvents; for example, surfactants, applicable surfactants include emulsifiers, dispersants or wetting agents with ionic or non-ionic properties, or mixtures of these surfactants; these surfactants are suitable for agricultural milks such as fatty alcohol polyoxyethylene ethers, polyoxyethylene alkyl aryl ethers, polyoxyethylene higher fatty acid esters, polyoxyethylene alcohol or phenol phosphates, fatty acid esters of polyols, sodium alkyl aryl sulfonates, naphthalenesulfonic acid polymers, etc.

[0010] Furthermore, the mass ratio of ciflumizone to polymethoxysilane is 1:5 to 5:2. Ensuring the ratio of ciflumizone to polymethoxysilane ensures the particle size of the composition particles, preventing the composition from becoming too large after mixing, thereby affecting its control effect. Excessive use of polymethoxysilane reduces the cost of the composition, while insufficient use of polymethoxysilane results in overly large particles, affecting the control effect of the composition. Therefore, adding polymethoxysilane within this range can effectively promote the quick-acting properties of the composition.

[0011] Furthermore, the mass ratio of cyflumizone and polymethoxysilane is 4:3 to 5:3.

[0012] Furthermore, the dosage form of the composition is any one of wettable powder, suspension, emulsifiable concentrate, dispersible liquid, dispersible granule, microemulsion, aqueous emulsion, sustained-release agent, microcapsule suspension and bait.

[0013] Furthermore, the composition is a dispersible liquid, which includes active ingredient A, active ingredient B, adjuvants and excipients, which are uniformly mixed and stirred in a stirrer at 30-50 revolutions per minute for 30 minutes to form a stable uniform liquid phase system.

[0014] Furthermore, the active ingredient A is 10% cyprodinil, the active ingredient B is 4% triflumuron, the adjuvant is 7.5% polymethoxysilane, and the excipients include 3% castor oil polyoxyethylene ether, 20% dimethylformamide, and 3% sodium dodecylsulfonate, and ethylene glycol is used to make up to 100%.

[0015] An application of the above-mentioned nematode killing composition, wherein the composition is used for preventing and controlling nematode diseases on pine wood.

[0016] Furthermore, the composition is diluted 20 to 1000 times and then applied to the bark of pine trees, injected into pine trees through drilling holes, or spread on the soil.

[0017] Trifluoropyridinamine: Trifluoropyridinamine has a novel chemical structure, with both pyridineamide and cyclobutyl structures in the molecule. This product exists in four crystalline forms, of which B and C are stable. The IUPAC chemical name of trifluoropyridinamine is: A mixture of 80% to 100% N-[(1S,2S)-2-(2,4-dichlorophenyl)cyclobutyl]-2-(trifluoromethyl)pyridine-3-carboxamide and 20% to 0% of the (1R,2R)-enantiomer; CAS registration number: 1460292-16-3; molecular formula: C17H13Cl2F3N2O; relative molecular mass: 389.20; structural formula:

[0018] The structural formula of trifluridine

[0019] Trifluoperazine is a SDHI-class fungicide and nematicide. It acts as a pathogen respiration inhibitor, interfering with the tricarboxylic acid cycle on Complex II of the respiratory electron transport chain. This inhibits mitochondrial function, preventing energy production and growth, ultimately leading to pathogen death. Trifluoperazine also inhibits succinate dehydrogenase activity, disrupting ATP production in the mitochondria of plant pathogenic nematodes, and is effective against a wide variety of nematodes, including tomato root-knot nematodes.

[0020] Triflupyramide is a broad-spectrum, highly effective pesticide that provides long-lasting protection against hard-to-detect but destructive nematodes and soil-borne diseases, particularly Fusarium spp. It not only protects plant roots but also travels to the above-ground parts of the plant to combat early-stage diseases.

[0021] Cyproflanilide is a new insecticide with the common name cyproflanilide and the chemical name 3'-[(2-bromo-4-[1,2,2,2-tetrafluoro-1-(trifluoromethyl)ethyl]-6-(trifluoromethyl)phenyl|amino)carbonyl]-N-(cyclopropanemethyl)-2',4-difluorobenzanilide. CAS registration number: 2375110-88-4; molecular formula: C28H17BrF12N2O2. The structural formula of cyproflanilide is as follows:

[0022] Cyprodinil structure

[0023] Cycloflumizone is a diamide insecticide with a presumed mechanism of action as a γ-aminobutyric acid (GABA) chloride channel allosteric enzyme modulator. It is classified as IRAC classification group 30.

[0024] The present invention has the following characteristics:

[0025] 1. By combining the insecticide cyprodinil and the fungicide triflumuron, a superposition or synergistic effect can be achieved, which effectively improves the rapidity of the prevention and control of pine wood nematodes. At the same time, it ensures the lasting effect on pine wood nematodes, avoids the re-reproduction of eggs, and plays a preventive role in pine wood, ensuring the healthy growth of pine plants.

[0026] 2. The use of polymethoxysilane adjuvant ensures the penetration effect of the composition, improves the conduction of the composition in the plant, and can promote the insecticidal effect of the combination of cyflumizone and triflumuron.

[0027] The present invention is further described in detail below in conjunction with specific embodiments. DETAILED DESCRIPTION

[0028] Preparation Example 1 Preparation A

[0029] A 20% ciprofloxacin·triflupirid dispersible liquid comprises, by weight, 10% ciprofloxacin, 10% triflupirid, 3% castor oil polyoxyethylene ether, 5% polymethoxysilane, 2% calcium dodecylbenzenesulfonate, and ethylene glycol to 100%. The active ingredients and other additives are mixed and dispersed in a stirrer at 30-50 rpm for 30 minutes to obtain a stable and uniform emulsion system, thereby preparing the 20% ciprofloxacin·triflupirid dispersible liquid.

[0030] Preparation Example 2 Preparation B

[0031] A 16% ciprofloxacin·triflupirid dispersible liquid comprises, by weight, 10% ciprofloxacin, 6% triflupirid, 3% castor oil polyoxyethylene ether, 5% polymethoxysilane, 2% calcium dodecylbenzenesulfonate, and ethylene glycol to 100%. The active ingredients and other additives are mixed and dispersed in a stirrer at 30-50 rpm for 30 minutes to obtain a stable and uniform emulsion system, thereby preparing the 16% ciprofloxacin·triflupirid dispersible liquid.

[0032] Preparation Example 3 Preparation C

[0033] A 14% ciprofloxacin·triflupirid dispersible liquid comprises, by weight, 10% ciprofloxacin, 4% triflupirid, 3% castor oil polyoxyethylene ether, 5% polymethoxysilane, 2% calcium dodecylbenzenesulfonate, and ethylene glycol to 100%. The active ingredients and other additives are mixed and dispersed in a stirrer at 30-50 rpm for 30 minutes to obtain a stable and uniform emulsion system, thereby preparing the 14% ciprofloxacin·triflupirid dispersible liquid.

[0034] Preparation Example 4 Preparation D

[0035] A 16% ciprofloxacin·triflupirid dispersible liquid comprises, by weight, 12% ciprofloxacin, 4% triflupirid, 3% castor oil polyoxyethylene ether, 5% polymethoxysilane, 2% calcium dodecylbenzenesulfonate, and ethylene glycol to 100%. The active ingredients and other additives are mixed and dispersed in a stirrer at 30-50 rpm for 30 minutes to obtain a stable and uniform emulsion system, thereby preparing the 16% ciprofloxacin·triflupirid dispersible liquid.

[0036] Preparation Example 5 Preparation E

[0037] A 20% ciprofloxacin·triflupirid dispersible liquid comprises, by weight, 16% ciprofloxacin, 4% triflupirid, 3% castor oil polyoxyethylene ether, 8% polymethoxysilane, 2% calcium dodecylbenzenesulfonate, and ethylene glycol to 100%. The active ingredients and other additives are mixed and dispersed in a stirrer at 30-50 rpm for 30 minutes to obtain a stable and uniform emulsion system, thereby preparing the 20% ciprofloxacin·triflupirid dispersible liquid.

[0038] Preparation Example 6 Preparation C1

[0039] A 14% ciprofloxacin·triflupirid dispersible liquid comprises, by weight, 10% ciprofloxacin, 4% triflupirid, 3% castor oil polyoxyethylene ether, 10% polymethoxysilane, 2% calcium dodecylbenzenesulfonate, and ethylene glycol to 100%. The active ingredients and other additives are mixed and dispersed in a stirrer at 30-50 rpm for 30 minutes to obtain a stable and uniform emulsion system, thereby preparing the 14% ciprofloxacin·triflupirid dispersible liquid.

[0040] Preparation Example 7 Preparation C2

[0041] A 14% ciprofloxacin·triflupirid dispersible liquid comprises, by weight, 10% ciprofloxacin, 4% triflupirid, 3% castor oil polyoxyethylene ether, 15% polymethoxysilane, 2% calcium dodecylbenzenesulfonate, and ethylene glycol to 100%. The active ingredients and other additives are mixed and dispersed in a stirrer at 30-50 rpm for 30 minutes to obtain a stable and uniform emulsion system, thereby preparing the 14% ciprofloxacin·triflupirid dispersible liquid.

[0042] Preparation Example 8 Preparation F

[0043] A 10% ciprofloxacin dispersible liquid formulation comprises, by weight, 10% ciprofloxacin, 3% castor oil polyoxyethylene ether, 10% polymethoxysilane, 2% calcium dodecylbenzenesulfonate, and ethylene glycol to 100%. The active ingredient and other adjuvants are mixed and dispersed in a stirrer at 30-50 rpm for 30 minutes to obtain a stable and uniform emulsion system, thereby preparing the 10% ciprofloxacin dispersible liquid formulation.

[0044] Preparation Example 9 Preparation F1

[0045] A 4% trifluopyramide dispersible liquid formulation comprises, by weight, 4% trifluopyramide, 3% castor oil polyoxyethylene ether, 15% polymethoxysilane, 2% calcium dodecylbenzenesulfonate, and ethylene glycol to 100%. The active ingredient and other additives are mixed and dispersed in a stirrer at 30-50 rpm for 30 minutes to obtain a stable and uniform emulsion system, thereby preparing the 4% trifluopyramide dispersible liquid formulation.

[0046] Test on the synergistic effect of ciprofloxacin and triflupyridam against isolated pine wood nematodes:

[0047] Pine wood nematodes were collected from the pine forest (Pinus massoniana) of Shangfu Village Committee, Yulao Town, Fengkai County, Zhaoqing City, Guangdong Province. Wood from diseased and dead trees was thinly sliced ​​and isolated using the Behmann funnel method. After microscopic examination confirmed the nematodes as pine wood nematodes (identification methods were based on the Pine Wood Nematode Quarantine Technical Procedures published by the Jiangsu Provincial Forestry Bureau), 10 female adults and 4 male adults were selected and placed on potato dextrose agar (PDA) plates infested with the fungus Botrytis cinerea and cultured in a 25°C incubator. After the nematodes had largely consumed the mycelium, they were further cultured. Finally, the nematodes cultured on the Botrytis cinerea plates were washed with sterile water and a nematode suspension at a concentration of 50-200 nematodes / mL was prepared for use in the experiment. The control efficacy of the active ingredient against pine wood nematodes was determined using the contact toxicity assay. 0.5 ml of the drug solution and 0.5 ml of the nematode suspension were added to a 24-well plate. Each treatment was repeated 3 times. The 24-well plates were sealed with sealing film and placed in a 25°C incubator for culture. The suspensions of nematodes in each treatment and control were then poured into a 500-mesh sieve to filter out the drug solution. The nematodes were rinsed with sterile water and recovered in a culture dish. Nematode mortality was examined microscopically 1, 3, and 7 days after drug administration. The statistical data and the pine wood nematode reduction rate were calculated as the control effect. In addition, polymethoxysilane alone has almost no control effect on certain nematodes, which is not reflected in the data table. Control agent 1 and control agent 2 are both conventional agents on the market. The specific test agents used are shown in the following table:

[0048]

[0049]

[0050] Control effect (%) = 100 × number of dead nematodes / number of tested nematodes; and calculate the average control effect of three repetitions.

[0051] The indoor measurement data are shown in Table 1 below:

[0052] Table 1 Statistics of the average control effect of indoor pine wood nematodes after three repetitions

[0053] Composition Dilution multiple 1 day after taking the medicine 3 days after taking the medicine 7 days after taking the medicine 20% cyprodinil·trifluanid 1000 48.3 64.8 77.4 16% cyprodinil·trifluanid 1000 62.4 73.7 83.3 14% cyprodinil·trifluanid 1000 86.3 92.6 96.8 16% cyprodinil·trifluanid 1000 71.6 81.3 87.6 20% cyprodinil·trifluanid 1000 57.5 61.9 75.8 14% cyprodinil·trifluanid 1000 81.6 88.4 92.6 14% cyprodinil·trifluanid 1000 77.4 82.7 90.3 10% cyprodinil 1000 37.8 54.7 65.3 4% trifluridine 1000 36.2 59.6 71.2 5% avermectin 1000 72.3 83.4 86.6 5% Fluopyram 1000 65.5 76.2 85.3 Ck clear water 0 0 0 0

[0054] From the above indoor test results, it can be seen that the control effect of 14% cyprodinil 10·triflupyram 4 on pine wood nematodes in the present invention significantly plays a superimposed or synergistic role, and the control effect of the combination of the two is significantly higher than that of using them alone. After the combination, the control of pine wood nematodes reaches more than 95% 7 days after the application, which is 30-40 percentage points higher than the control effect of using either agent alone; at the same time, the control effect of the combination of the two on pine wood nematodes is significantly higher than the control effect of conventional agents avermectin and fluopyram on pine wood nematodes; therefore, from the above data, it can be seen that the quick-acting effect of the combination of the two on pine wood nematodes is significantly improved, and the insecticidal control effect reaches more than 85% one day after the application, and the insecticidal control effect reaches more than 90% three days after the application.

[0055] Table 2 Original data of the three-repeated control effect of indoor pine wood nematodes

[0056]

[0057]

[0058] The pine forest (Pinus massoniana) of Shangfu Village Committee, Yulao Town, Fengkai County, Zhaoqing City, Guangdong Province was selected. The diluted pesticide was injected into each treatment. Each treatment was set up with 3 replicates. Each replicate selected 3-6 diseased pine trees. Before the application, the pine wood nematode base number of each replicate was randomly collected and counted. During the collection and investigation, pine wood was randomly collected from 5 points and the nematodes in the pine wood were screened. After the base number was investigated, the first application was performed by injection. The second application was performed after an interval of 7 days. Investigations were conducted 14 days, 30 days, and 60 days after the application, and the statistical data was used to calculate the control effect.

[0059] Control effect (%) = 100 × number of dead nematodes / number of tested nematodes; the average of the three replicates was used as the final control effect. The specific results are shown in Table 3:

[0060] Table 3 Statistics of the average control effect of three replicates of pine wood nematodes outdoors

[0061]

[0062] As can be seen from the above table, the control effect of the cyprosulfuron·trifluanid combination preparation AE on pine wood nematodes is significantly better than the control effect of preparations F and F1 used alone on pine wood nematodes. At the same time, compared with the control agent, the duration of the control effect of the cyprosulfuron·trifluanid combination on pine wood nematodes is significantly better than that of the conventional control agent. The control effect of preparation C on pine wood nematodes can reach more than 95% 14 days after the application, the control effect can reach more than 89% 30 days after the application, and the control effect can be maintained at more than 75% 60 days after the application, which is significantly better than the control effect of conventional agents on pine wood nematodes. In addition, the present invention uses cyprosulfuron·trifluanid in combination with a specific adjuvant to improve the penetration and conduction ability of the agent in pine wood plants. From the comparison of preparations C, C1 and C2, it can be seen that the adjuvant can play a certain promoting and enhancing role in the control effect of the cyprosulfuron·trifluanid combination preparation for pine wood nematodes.

[0063] Table 4 Statistical table of original data of three-time control effect of outdoor pine wood nematode

[0064]

[0065] Preparation Example 10 Preparation C4

[0066] The invention discloses a 14% ciprofloxacin·triflupyridam emulsifiable concentrate, whose raw material formula, calculated by weight, is 10% ciprofloxacin, 4% triflupyridam, 10% DMF, and S-150 is supplemented to 100%. The active ingredient and other adjuvants are mixed and dispersed in a stirrer at 30-50 revolutions per minute for 30 minutes to obtain a stable and uniform emulsion system, thereby preparing the 14% ciprofloxacin·triflupyridam emulsifiable concentrate.

[0067] Preparation Example 11 Preparation C5

[0068] The invention discloses a 14% ciprofloxacin·triflupyridam wettable powder, whose raw material formula, calculated by weight, is 10% ciprofloxacin, 4% triflupyridam, 10% white carbon black, 5% sodium lauryl sulfate, 6% calcium lignin sulfonate, and kaolin to make up 100%. The active ingredients and other adjuvants are mixed, stirred and dispersed in a blender at 30-50 revolutions per minute for 30 minutes, and pulverized in a jet mill. After collection, a uniform wettable powder is obtained, thereby preparing the 14% ciprofloxacin·triflupyridam wettable powder.

[0069] In indoor tests, different dosage forms of 14% ciprofloxacin·trifluamid, namely, formulations C, C4 and C5, were compared. Formulation C had the best control effect on pine wood nematodes, followed by C4 and C5. This was because the particle size of formulation C was smaller than that of the other two formulations, making it more effective in controlling pine wood nematodes.

[0070] The above are preferred embodiments of the present invention and the technical principles used therein. For those skilled in the art, any obvious changes such as equivalent transformations, simple replacements, etc. based on the technical solution of the present invention, without departing from the spirit and scope of the present invention, are within the scope of protection of the present invention.

Claims

1. A preparation for preventing and treating pine wood nematode disease, characterized in that: The preparation is a 14% cyprosulfamide and triflupyridam dispersible liquid. Its raw material formula, calculated by weight, is 10% cyprosulfamide, 4% triflupyridam, 3% castor oil polyoxyethylene ether, 5% polymethoxysilane, 2% calcium dodecylbenzenesulfonate, and ethylene glycol is supplemented to 100%.

2. A use of the preparation for preventing and controlling pine wood nematode disease according to claim 1, characterized in that: The preparation is used for preventing and controlling pine wood nematode disease.

3. The use of the preparation for preventing and controlling pine wood nematode disease according to claim 2, characterized in that: After diluting the composition 20 to 1000 times, apply it on the bark of pine trees, inject it into pine trees through drilling, or spread it on the soil.

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