Source fluid tank with temperature control function

Abstract

The application discloses a source fluid pool with a temperature control function, comprising a source fluid pool main body structure and an external temperature control system, wherein the source fluid pool main body structure and the external temperature control system utilize a mild water bath heating mode to provide a suitable temperature for a liquid containing biological active substances such as cells, proteins and growth factors in an inner porous plate, meet the needs of ultrasonic pipetting technology in the biochemical field, have the advantages of miniaturization and high throughput, and can realize accurate temperature control of the liquid to be transferred while meeting the effective transmission of acoustic energy and guaranteeing the stability of liquid transfer.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of liquid temperature control of ultrasonic pipetting, and particularly relates to a source fluid pool with temperature control function. BACKGROUND

[0002] As a device for loading discrete fluid in the ultrasonic pipetting process, the optimized design of the source fluid pool structure is crucial for the ultrasonic pipetting system to realize the array operation of micro liquid. At present, the design forms of the source fluid pool structure are various, and most of the source fluid pools have the advantages of low sample volume processing capacity, miniaturization and high throughput, but there are still some challenges in the liquid transfer process, especially for temperature-sensitive liquids and biologically active materials. This temperature-sensitive characteristic makes it necessary to accurately control the temperature of the source fluid pool during the liquid transfer process to meet the specific requirements of the liquid and biologically active materials for temperature conditions in the rapid and efficient transfer process. For example, the methyl methacrylated gelatin (GelMA for short) commonly used in the process of biological 3D printing has temperature sensitivity, and forms a reversible physical gel at low temperature (room temperature or refrigerator cold storage in the dark), and can restore to liquid state by heating (usually 30-40 DEG C). In addition, the ultrasonic pipetting technology has been widely used in the fields of cell 3D culture, tissue engineering and biological 3D printing, and most of the related fields involve the transfer of some biologically active substances such as biological macromolecules and cells. These active substances need to be maintained at an appropriate temperature to maintain their activity, but the existing source fluid pool cannot provide an appropriate temperature for the liquid containing these active substances, so it is difficult to achieve efficient transfer of the target liquid.

[0003] At present, the source fluid pool structure used in ultrasonic pipetting technology is mainly based on multi-well plates. If a heating device is used to directly heat the multi-well plate or the liquid to be transferred, it will bring many problems: (1) The heating device is directly attached to the outside of the fluid pool. In order to ensure rapid heating, a relatively high heating temperature is usually used, which may cause damage and deformation of the fluid pool; (2) The heating device and temperature sensor are directly placed inside the fluid pool. Since the liquid to be transferred is in contact with the device, it may contaminate the liquid, and there is also a risk of corroding the device; (3) Whether the heating device is placed directly outside or inside the fluid pool, it is not conducive to the miniaturization design of the fluid pool. Although the source fluid pool is made of materials such as borosilicate glass and quartz which have high temperature resistance and high light transmittance, it can solve the problem of damage and deformation of the fluid pool at high heating temperature, but these materials will cause a large amount of energy loss in the transmission of ultrasonic waves. In order to overcome the above problems, it is necessary to consider a more precise and safe heating method for the source fluid pool while selecting appropriate fluid pool materials. SUMMARY

[0004] The technical problem to be solved by the present application is to provide a source fluid pool with temperature control function.

[0005] To solve the above technical problems, the technical scheme provided by the present application is as follows:

[0006] The source fluid pool with temperature control function comprises a source fluid pool main body structure and an external temperature control system, the source fluid pool main body structure (1) is a double-layer nested structure, which is composed of an inner-layer multi-well plate (2) for loading liquid to be transferred and a cuboid outer shell (3) for water bath heating, and the inner-layer multi-well plate (2) is internally provided with micro-holes arranged in rows and columns; the cuboid outer shell (3) is respectively provided with a liquid inlet (4) and a liquid outlet (5) on two sides, and the external temperature control system comprises a first temperature sensor (6) for monitoring the temperature of circulating liquid at the liquid inlet and the liquid outlet of the source fluid pool main body structure, a peristaltic pump (8) for driving the circulation flow of liquid, a heater (12) for heating the circulating liquid, and a heat-conducting water pipe (7) for connecting the above parts, so that the external temperature control system is connected with the liquid inlet (4) and the liquid outlet (5) of the source fluid pool through the heat-conducting water pipe (7).

[0007] Preferably, the source fluid pool with temperature control function comprises a heater (12) including a heating body (13), a temperature control display device (14), a water shortage alarm (15), a second temperature sensor (16), a heating box (18), and a heating box cover plate (19), the heating box cover plate (19) is provided with a liquid injection port (17), the heating box cover plate (19) is fixed on the heating box (18) to form a closed cavity for slowing down the evaporation and cooling of liquid in the heating box, the heating box (18) is internally provided with the heating body (13), the water shortage alarm (15), and the second temperature sensor (16), the side wall of the heating box (18) is provided with a heating box liquid outlet (21) and a heating box liquid inlet (22), and the outer side of the heating box cover plate (19) is provided with the temperature control display device (14), which is electrically connected with the heating body (13) and the temperature sensor (16).

[0008] The liquid circulation passage of the external temperature control system is as follows: under the driving of the peristaltic pump (8), the circulating liquid in the heater (12) passes through the peristaltic pump (8), enters the liquid inlet (4) of the source fluid pool main body structure, fills the interlayer of the source fluid pool main body structure, and then returns to the heater through the liquid outlet (5) of the source fluid pool main body structure, so as to ensure the circulation flow of liquid.

[0009] The temperature control display device (14) of the external temperature control system is provided with a heating switch and a temperature adjustment button. The heating switch serves as the total switch of the heater, and the target heating temperature of the heater is set through the temperature adjustment button. The working state of the heating body is controlled to heat the liquid in the heating tank (18), and the temperature of the liquid in the heating tank is measured by the second temperature sensor (16). The working state of the heating body (13) is feedback controlled to heat the liquid in the heating tank to reach and maintain the target temperature set by the temperature adjustment button. The external temperature control system injects liquid into the heating tank (18) through the liquid injection port (17) of the heating tank as the circulating heating liquid of the external temperature control system.

[0010] Preferably, the source fluid pool with temperature control function is provided with an exhaust hole (20) on the cover plate (19) of the heating tank. The exhaust hole (20) functions as a ventilation hole when liquid is injected into the heating tank (18) through the liquid injection port (17) of the heating tank and when the interlayer of the source fluid pool is filled with liquid through the peristaltic pump (8).

[0011] Preferably, the source fluid pool with temperature control function is provided with an exhaust hole (20) on the cover plate (19) of the heating tank. The exhaust hole (20) functions as a ventilation hole when liquid is injected into the heating tank (18) through the liquid injection port (17) of the heating tank and when the interlayer of the source fluid pool is filled with liquid through the peristaltic pump (8).

[0012] Preferably, the source fluid pool with temperature control function is provided with an exhaust hole (20) on the cover plate (19) of the heating tank. The exhaust hole (20) functions as a ventilation hole when liquid is injected into the heating tank (18) through the liquid injection port (17) of the heating tank and when the interlayer of the source fluid pool is filled with liquid through the peristaltic pump (8).

[0013] Preferably, the source fluid pool with temperature control function is provided with an exhaust hole (20) on the cover plate (19) of the heating tank. The exhaust hole (20) functions as a ventilation hole when liquid is injected into the heating tank (18) through the liquid injection port (17) of the heating tank and when the interlayer of the source fluid pool is filled with liquid through the peristaltic pump (8).

[0014] Preferably, the source fluid pool with temperature control function, the outer edge of the inner layer porous plate (2) and the edge of the top of the cuboid shell (3) are designed as a step structure, and the step structures of the two are matched in size, that is, the length (L4) of the step structure = the length (L5) of the step structure of the porous plate = the length (L6) of the step structure of the cuboid shell, the width (W4) of the step structure of the porous plate = the width (W7) of the step structure of the cuboid shell, and the height (H5) of the step structure = the height (H6) of the step structure of the porous plate = the height (H7) of the step structure of the cuboid shell, so that the cuboid shell supports the inner porous plate and ensures the sealing connection of the double-layer nested structure through adhesive.

[0015] Preferably, the source fluid pool with temperature control function, the materials of the inner layer porous plate (2) and the cuboid shell (3) are polystyrene, polyethylene or cyclic hydrocarbon, which have good biological compatibility, good acoustic characteristics and high heat resistance, meet the needs of ultrasonic pipetting technology in the biochemical field and ensure the effective transmission of acoustic energy and the stability of liquid transfer, and realize precise heating of the inner layer porous plate and miniaturized design of the source fluid pool.

[0016] Preferably, the source fluid pool with temperature control function, the ultrasonic transducer (23) is arranged below the source fluid pool main structure (1), the coupling medium between the ultrasonic transducer and the cuboid shell (3) and the circulating liquid in the source fluid pool main structure sandwich are deionized water or ultrapure water commonly used in laboratories, which have similar acoustic impedance with the materials of the double-layer fluid pool, meeting the needs of acoustic impedance matching in the ultrasonic pipetting process.

[0017] Preferably, the source fluid pool with temperature control function, the liquid inlet (4) is arranged at one end of the lower bottom corner of the cuboid shell, and the liquid outlet (5) is arranged at the top corner near the diagonal line of the liquid inlet, so that the circulating liquid adopts the mode of entering from the bottom and flowing out from the top, ensuring that the gas in the sandwich of the double-layer nested structure of the source fluid pool is emptied as much as possible in the initial injection stage of the circulating liquid, so that the circulating liquid fills the sandwich of the source fluid pool.

[0018] Preferably, the source fluid pool with temperature control function, the heating body (13) is fixed to the lower surface of the heating box cover plate (19).

[0019] Preferably, the source fluid pool with temperature control function, the temperature control display device (14) is fixed to the heating box cover plate (19).

[0020] Preferably, the source fluid pool with temperature control function, the heat-conducting water pipe (7) is provided with a switch valve (11) for controlling the flow of liquid at the outlet of the heater.

[0021] Preferably, the source fluid pool with temperature control function, the low water alarm (15) is located at the lowest water level of the interlayer of the source fluid pool filled with liquid, and the low water alarm prompts when the liquid in the heating box is lower than the lowest water level.

[0022] The source fluid pool with temperature control function, the temperature control system realizes the temperature control of the ultrasonic transfer liquid loaded in the inner porous plate of the source fluid pool main structure through the following process:

[0023] (1) Inject liquid into the heater (12) through the liquid injection port (17), and set the temperature of the heater through the temperature adjustment button on the temperature control display:

[0024] (2) When the liquid in the heating box is heated to the set temperature, adjust the flow rate of the peristaltic pump (8) so that the liquid to be transferred in the inner porous plate (2) is heated to the specified temperature; The first temperature sensor (6) is arranged at the liquid inlet and liquid outlet of the source fluid pool main structure, and the temperature of the liquid inlet and liquid outlet of the source fluid pool is used to dynamically adjust the temperature of the heater and the flow rate of the peristaltic pump, so as to realize precise temperature control.

[0025] Technical effects

[0026] The source fluid pool with temperature control function, the source fluid pool main structure and its external temperature control system use mild water bath heating method to provide suitable temperature for the liquid containing biological active substances such as cells, proteins and growth factors in the inner porous plate, meet the demand of ultrasonic pipetting technology in biochemical field, not only has the advantages of miniaturization and high throughput, but also can realize precise temperature control of the liquid to be transferred while meeting the effective transmission of acoustic energy and ensuring the stability of liquid transfer. Specifically,

[0027] The external temperature control system uses the driving force of the peristaltic pump to deliver the liquid heated to the specified temperature in the heater to the interlayer of the source fluid pool main structure, and provides uniform heating for the inner porous plate. The liquid absorbed by heat returns to the heater, so as to realize precise temperature control of the inner porous plate; The circulating heating process of the liquid in the external temperature control system is conducive to maintaining the temperature of the liquid in the interlayer of the double-layer source fluid pool, and further realizing precise and stable control of the temperature of the ultrasonic transfer liquid. BRIEF DESCRIPTION OF DRAWINGS

[0028] Figure 1 It is the overall structure schematic diagram of the source fluid pool with temperature control function.

[0029] Figure 2 It is the schematic diagram of the source fluid pool main structure of the source fluid pool with temperature control function.

[0030] Figure 3It is the inner layer porous plate structure and single micro-hole structure diagram of the source fluid pool with temperature control function.

[0031] Figure 4 It is the assembly way diagram of the inner layer micro porous plate and cuboid shell of the source fluid pool with temperature control function.

[0032] Figure 5 It is the top view of the inner layer micro porous plate of the source fluid pool with temperature control function.

[0033] Figure 6 It is the top view of the cuboid shell of the source fluid pool with temperature control function.

[0034] Figure 7 It is the front view of the inner layer micro porous plate of the source fluid pool with temperature control function.

[0035] Figure 8 It is the front view of the cuboid shell of the source fluid pool with temperature control function.

[0036] In the figure: 1-source fluid pool main structure, 2-inner layer porous plate, 3-cuboid shell, 4-source fluid pool liquid inlet, 5-source fluid pool liquid outlet, 6-first temperature sensor, 7-heat conduction water pipe, 8-peristaltic pump, 9-peristaltic pump liquid inlet, 10-peristaltic pump liquid outlet, 11-switch valve, 12-heater, 13-heating body, 14-temperature control display device, 15-water shortage alarm, 16-second temperature sensor, 17-liquid injection port, 18-heating box, 19-heating box cover plate, 20-vent hole, 21-heating box liquid outlet, 22-heating box liquid inlet, 23-ultrasonic transducer, W1-single micro-hole bottom width, H1-single micro-hole internal height, L1-internal length of cuboid shell, H2-external height of single micro-hole, L2-external length of cuboid shell, H3-internal height of cuboid shell, L3-internal length of porous plate step, H4-external height of cuboid shell, L4-length of step structure, H5-step structure height, D1-casing thickness of cuboid shell, W2-internal width of porous plate step, W3-external width of porous plate step, W4-step structure width of porous plate, L5-step structure length of porous plate, H6-step structure height of porous plate, W5-internal width of cuboid shell, W6-external width of cuboid shell, W7-step structure width of cuboid shell, L6-step structure length of cuboid shell, H7-step structure height of cuboid shell DETAILED DESCRIPTION

[0037] The source fluid pool device with temperature control function is described in detail below in combination with examples and drawings.

[0038] As Figures 1-8 shown, the source fluid pool with temperature control function includes a source fluid pool main structure and an external temperature control system, the source fluid pool main structure 1 is a double-layer nested structure, which is composed of an inner-layer multi-well plate 2 loaded with liquid to be transferred and a cuboid shell 3 for water bath heating, and the inner-layer multi-well plate 2 is internally provided with micro-holes arranged in rows and columns; the cuboid shell 3 is respectively provided with a liquid inlet 4 and a liquid outlet 5 on two sides, the liquid inlet 4 is arranged at the lower corner near the bottom of the cuboid shell 3, and the liquid outlet 5 is arranged at the top corner near the diagonal line of the liquid inlet; the external temperature control system includes a first temperature sensor 6 for monitoring the temperature of the circulating liquid at the liquid inlet and the liquid outlet of the source fluid pool main structure, a peristaltic pump 8 for driving the circulation of the liquid, a heater 12 for heating the circulating liquid, and a heat-conducting water pipe 7 for connecting the above parts; the heat-conducting water pipe 7 is provided with a switch valve 11 for controlling the flow of liquid at the outlet of the heater; the external temperature control system is connected with the liquid inlet 4 and the liquid outlet 5 of the source fluid pool through the heat-conducting water pipe 7; the driving force of the peristaltic pump makes the whole system a liquid circulation and heating system; the source fluid pool main structure 1 is provided with an ultrasonic transducer 23 below; the coupling medium between the ultrasonic transducer and the cuboid shell 3 and the circulating liquid in the interlayer of the source fluid pool main structure are selected from deionized water or ultrapure water commonly used in laboratories; the similar acoustic impedance between the deionized water or ultrapure water and the material of the double-layer fluid pool meets the demand of acoustic impedance matching in the ultrasonic pipetting process.

[0039] The heater 12 comprises a heating body 13, a temperature control display device 14, a water shortage alarm 15, a second temperature sensor 16, a heating tank 18 and a heating tank cover plate 19, the heating tank cover plate 19 is provided with a liquid injection port 17 and an exhaust hole 20, the exhaust hole 20 functions as a vent when liquid is injected into the heating tank 18 through the liquid injection port 17 of the heating tank and when the interlayer of the source fluid pool is filled with liquid through the peristaltic pump 8, the heating tank cover plate 19 is fixed on the heating tank 18 to form a closed cavity for slowing down the evaporation and cooling of the liquid in the heating tank, the heating tank 18 is provided with the heating body 13, the water shortage alarm 15 and the second temperature sensor 16, the heating body 13 is fixed on the lower surface of the heating tank cover plate 19, the water shortage alarm 15 is located at the lowest water level at which the interlayer of the source fluid pool is filled with liquid, and the water shortage alarm prompts when the liquid in the heating tank is lower than the lowest water level, the side wall of the heating tank 18 is provided with a heating tank liquid outlet 21 and a heating tank liquid inlet 22, the upper surface of the heating tank cover plate 19 is fixed with the temperature control display device 14, which is electrically connected with the heating body 13 and the temperature sensor 16, the heating switch and the temperature adjustment button are arranged on the temperature control display device 14, the heating switch is used as the main switch of the heater, the target heating temperature of the heater is set through the temperature adjustment button, the working state of the heating body is controlled to heat the liquid in the heating tank 18, the second temperature sensor 16 is used to measure the temperature of the liquid in the heating tank, and the working state of the heating body 13 is fed back to control the heating of the liquid in the heating tank to reach and maintain the target temperature set by the temperature adjustment button. The external temperature control system injects liquid into the heating tank 18 through the liquid injection port 17 of the heating tank as the circulating heating liquid of the external temperature control system. The liquid circulation path of the external temperature control system is that, under the drive of the peristaltic pump 8, the circulating liquid in the heater 12 flows into the source fluid pool liquid inlet 4 through the peristaltic pump liquid inlet 9 and the peristaltic pump liquid outlet 10, fills the interlayer of the main structure of the source fluid pool, and then returns to the heater through the source fluid pool liquid outlet 5 to ensure the circulation of the liquid.

[0040] Specifically, the double-layer source fluid pool main structure is as shown in Figure 2 The double-layer source fluid pool main structure is composed of an inner porous plate 2 loaded with liquid to be transferred and a rectangular cuboid outer shell 3 used for water bath heating. Taking a 3×2 hole plate array arrangement as an example, the structure of the inner porous plate of the double-layer source fluid pool main structure and a single micro-hole is as shown in Figure 3The single micro-well internal height (micro-well depth) H1 is greater than the single micro-well bottom width W1, adopts an inverted trapezoidal or inverted conical structure with a certain angle θ between the side wall and the bottom surface, and satisfies 90° < θ < 180°, so as to reduce the liquid meniscus and the side wall angle, ensure stable droplet transfer, and effectively reduce the dead volume caused by the liquid meniscus in the micro-well, which cannot realize ultrasonic pipetting. At present, in the field of biological application, in order to realize high-throughput operation, commercial 1536-well plate structure is widely used. In this structure, the design of the single micro-well follows the principle that the ratio of the depth to the bottom width is greater than 3:1. In the implementation process of the present scheme, the single micro-well size of the internal inverted trapezoidal fluid pool is set to micro-well depth: bottom width > 3:1. In actual application, the cuboid shell and the inner layer multi-well plate are made of polystyrene material with good acoustic characteristics and continuous use temperature of about 60℃, and the maximum temperature is not more than 80℃.

[0041] The nesting mode of the inner layer multi-well plate of the double-layer fluid pool and the cuboid shell is as shown in Figure 4 The outer edge of the inner layer multi-well plate and the edge of the top of the cuboid shell are designed as a stepped structure, and the sizes of the stepped structures of the two are matched, so that the cuboid shell supports the inner layer multi-well plate, and the sealing and bonding of the double-layer nested structure are ensured by adhesive, which is convenient for the cuboid shell to support the inner layer multi-well plate and realize the bonding and sealing of the nested structure.

[0042] As shown in Figures 5-8 In order to reduce the energy loss of ultrasonic wave transmission caused by other factors (such as bubbles), the inner layer multi-well plate is in an inverted trapezoidal shape, and the size of the cuboid shell is set as follows: the internal height H3 of the cuboid shell is greater than the external height H2 of the single micro-well, and the internal length L1 of the cuboid shell and the internal width W5 of the cuboid shell are matched with the internal length L3 of the top multi-well plate step of the inner layer multi-well plate and the internal width W2 of the multi-well plate step, that is, L1 = L3 and W2 = W5. The outer edge of the inner layer multi-well plate and the edge of the top of the cuboid shell are designed as a stepped structure, and the sizes of the stepped structures of the two are matched, that is, the length L4 of the stepped structure = the length L5 of the stepped structure of the multi-well plate = the length L6 of the stepped structure of the cuboid shell, the width W4 of the stepped structure of the multi-well plate = the width W7 of the stepped structure of the cuboid shell, and the height H5 of the stepped structure = the height H6 of the stepped structure of the multi-well plate = the height H7 of the stepped structure of the cuboid shell, so that the cuboid shell supports the inner layer multi-well plate, and the sealing and bonding of the double-layer nested structure are ensured by adhesive.

[0043] The shell thickness D1 of the cuboid shell satisfies efficient propagation of sound energy, and in specific implementation, the thinnest thickness that can be processed is selected, and generally 1mm is selected. The size of the external length L2 of the cuboid shell and the external width W6 of the cuboid shell should satisfy perfect matching of the stepped structure, that is, even if L2 is greater than the sum of the internal length L3 of the porous plate step and the stepped structure length L5 of the porous plate on both sides, the external width W6 of the cuboid shell is slightly greater than the external width W3 of the porous plate step. In order to enable the water bath heating in the double-layer nested structure to provide uniform heating for the inner-layer porous plate, the external height H4 of the cuboid shell should be greater than the external height H2 of the single micro-hole.

[0044] The amount of liquid involved in the ultrasonic pipetting process belongs to the micro level, and the source fluid pool, the heater and the peristaltic pump are designed to be micro-sized, so as to achieve the purpose of energy saving and environmental protection. The specific size needs to be designed according to the actual demand.

[0045] In order to reduce the heat loss in the liquid circulation heating process, the heat-conducting water pipe adopts a double-layer pipe structure, and a heat preservation cavity is formed between the outer layer and the inner layer, and the heat preservation cavity is filled with heat preservation material.

[0046] In order to facilitate the understanding of the above-mentioned source fluid pool system with heating function, the working principle or operation mode in the actual process of the present application will be described in detail as follows:

[0047] In actual application, first, liquid is injected into the heating box through the liquid injection port 17 of the heating box; after sufficient liquid is injected, the target heating temperature is set through the temperature adjusting button and the heating body is controlled to work, so that the liquid in the heating box is heated to and maintained at the target temperature. Then, the interlayer of the double-layer fluid pool is subjected to liquid filling and gas emptying process: the switch valve 11 at the outlet of the heating box is opened, and the liquid in the heating box 18 is introduced into the interlayer of the source fluid pool under the driving of the peristaltic pump 8, while the gas in the interlayer of the source fluid pool is exhausted, so as to avoid energy loss in the ultrasonic wave transmission process. When the interlayer of the source fluid pool is filled with liquid, the liquid will return to the heating box 18 through the liquid outlet 5 of the source fluid pool, the heat-conducting water pipe 7 and the liquid inlet 22 of the heating box, and then the heat-conducting pipe 7, the heater 12, the peristaltic pump 8 and the source fluid pool main body structure 1 form a liquid circulation heating system.

[0048] The temperature control display device 14 of the heater 12 can set the target heating temperature of the heater through the temperature adjusting button, control the heating body 13 to work so that the liquid in the heating box 18 is heated, and at the same time, the second temperature sensor 16 is used to measure the liquid temperature in the heating box 18, and the working state of the heating body 13 is fed back to control, so that the liquid in the heating box 18 is heated to and maintained at the target temperature set by the temperature adjusting button. The water shortage alarm device 15 in the heater 12 is located at the lowest water level at which the interlayer of the source fluid pool is filled with liquid, and is used to monitor the water level in the heating box. When the water level is lower than the set value, the water shortage alarm device 15 alarms.

[0049] The liquid heated to a specified temperature is transported from the heating tank 18 to the interlayer of the source fluid pool through the heat conducting pipe 7 by using the peristaltic pump 8. The first temperature sensor 6 is arranged at the liquid inlet 4 and the liquid outlet 5 of the source fluid pool, and the temperature of the liquid passing through the liquid inlet 4 and the liquid outlet 5 can be displayed in real time. The temperature of the external heater and the flow rate of the peristaltic pump are dynamically adjusted according to the liquid temperature at the liquid inlet and the liquid outlet of the main structure of the source fluid pool, so that the optimal temperature control effect can be achieved. The circulating heating process of the liquid in the external temperature control system is beneficial to maintaining the temperature of the liquid in the interlayer of the double-layer source fluid pool, and then the precise and stable control of the temperature of the ultrasonic transferred liquid is realized.

[0050] The above only describes the preferred embodiments of the present application, and it should be noted that the ordinary skilled in the art can make several improvements and refinements without departing from the principles of the present application, and these improvements and refinements should also be considered as the protection scope of the present application.

Claims

1. A source fluid pool with temperature control function, characterized in that: The system includes a source fluid pool main structure and an external temperature control system. The source fluid pool main structure (1) is a double-layer nested structure, consisting of an inner porous plate (2) for loading the liquid to be transferred and a cuboid outer shell (3) for water bath heating. The inner porous plate (2) has rows and columns of micropores inside. The cuboid outer shell (3) has an inlet (4) and an outlet (5) on both sides. The outer edge of the inner porous plate (2) and the top edge of the cuboid outer shell (3) are both designed as stepped structures. The dimensions of the stepped structures of the two are matched, so that the cuboid shell supports the internal porous plate, and the double-layer nested structure is sealed by adhesive. The internal height (H1) of a single micropore in the inner porous plate (2) is greater than the bottom width (W1) of a single micropore. The sidewalls of the micropores are inclined at an angle θ to the bottom surface in an inverted trapezoidal or inverted conical structure, 90° < θ < 180°. The internal height (H3) of the cuboid shell is greater than the external height (H2) of a single micropore. The length (L1) and the internal width (W5) of the cuboid shell are matched with the internal length (L3) and the internal width (W2) of the porous plate step on the inner porous plate. An ultrasonic transducer (23) is provided below the main structure (1) of the source fluid pool. The coupling medium between the ultrasonic transducer and the cuboid shell (3) and the circulating liquid in the interlayer of the main structure of the source fluid pool are selected from deionized water or ultrapure water commonly used in the laboratory. Taking advantage of the similar acoustic impedance of the material of the double-layer fluid pool, the acoustic impedance matching requirement during ultrasonic liquid transfer is met. The external temperature control system includes a first temperature sensor (6) for monitoring the temperature of the circulating liquid at the inlet and outlet of the main structure of the source fluid pool, a peristaltic pump (8) for driving the liquid circulation, a heater (12) for heating the circulating liquid, and a hot water pipe (7) for connecting the above parts. The external temperature control system is connected to the inlet (4) and outlet (5) of the source fluid pool through the hot water pipe (7).

2. The source fluid pool with temperature control function according to claim 1, characterized in that: The heater (12) includes a heating element (13), a temperature control display device (14), a water shortage alarm (15), a second temperature sensor (16), a heating box (18), and a heating box cover (19). The heating box cover (19) is provided with a liquid injection port (17). The heating box cover (19) is fixed to the heating box (18) to form a closed cavity. The heating box (18) is provided with a heating element (13), a water shortage alarm (15), and a second temperature sensor (16). The heating box (18) is provided with a heating box outlet (21) and a heating box inlet (22) on its side wall. The heating box cover (19) is provided with a temperature control display device (14) on its outer side. The temperature control display device (14) is electrically connected to the heating element (13) and the second temperature sensor (16).

3. The source fluid pool with temperature control function according to claim 2, characterized in that: The heating box cover (19) is provided with an exhaust hole (20).

4. The source fluid pool with temperature control function according to claim 1, characterized in that: The inner porous plate (2) and the cuboid outer shell (3) are made of polystyrene, polyethylene or cyclic hydrocarbons.

5. The source fluid pool with temperature control function according to claim 1, characterized in that: One end of the liquid inlet (4) is located near the bottom corner of the rectangular shell, and the liquid outlet (5) is located near the top corner of the diagonal of the liquid inlet.

6. The source fluid pool with temperature control function according to claim 2, characterized in that: The heating element (13) is fixed on the lower surface of the heating box cover (19); the temperature control display device (14) is fixed on the heating box cover (19); and the water shortage alarm (15) is located at the lowest water level that fills the interlayer of the source fluid pool with liquid.

7. The source fluid pool with temperature control function according to claim 1, characterized in that: The hot water pipe (7) is equipped with a switch valve (11) for controlling the liquid flow at the heater outlet.

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