A composition for shampooing with refreshing and oil-controlling effects and preparation method thereof

Abstract

The invention belongs to the technical field of daily necessities, and specifically relates to a composition for shampooing with refreshing oil-controlling effect and a preparation method thereof, comprising the following components: Anemarrhena asphodeloides root extract, Hamamelis virginiana extract, Mentha haplocalyx extract, and Tuber magnatum extract. The four plant extracts are rationally combined to achieve a good balance between efficient oil control and safety and mildness, overcoming the disadvantage of the prior art shampooing with plant extracts as effective ingredients, which has a poor effect.

Description

Technical Field

[0001] The invention belongs to the technical field of daily necessities, and particularly relates to a composition for shampooing with refreshing and oil-controlling effects and a preparation method thereof. Background Art

[0002] With the diversification of scalp diseases and the increasing incidence of scalp diseases, scalp health has received more and more attention. The scalp is the basis of hair health. The sebaceous glands of the scalp secrete sebum. Appropriate sebum plays a nourishing role and is an important component of the scalp's defense layer. However, if the sebum secretion is too strong, the Malassezia fungi on the scalp that feed on oil will increase, leading to rashes, itching, increased dandruff, and even exudation of odor, and seborrheic dermatitis. Abnormal scalp sebum secretion can also easily lead to many common skin diseases such as tinea capitis and scalp eczema. People with oily scalp should choose shampoo products that suit them. The frequency of shampooing needs to be determined according to the nature of the skin, the working and living environment, and the season. However, excessive degreasing should not be done, which will stimulate the scalp to secrete more oil.

[0003] The functional ingredients usually contained in oil-control shampoo mainly include sulfate surfactants, amino acid surfactants, quaternary ammonium salts, salicylic acid, plant extracts and other ingredients. Sulfate surfactants have strong cleaning and degreasing power and can effectively remove scalp oil, while amino acid surfactants are relatively mild and more suitable for sensitive skin. Quaternary ammonium salts have bactericidal and antiseptic effects, and can also help hair maintain a certain degree of fluffiness. Salicylic acid can penetrate pores, dissolve old cuticles that clog pores, help improve pore blockage, and reduce oil secretion. Some plant extracts have degreasing power, and adding them to shampoo can provide natural cleaning power and mild oil control effects.

[0004] Plant extracts are usually added to shampoo as oil-control ingredients, which are gentler, suitable for different skin types, and more friendly to sensitive skin. However, plant extracts sometimes cannot meet the efficacy requirements, and the oil-control effect is limited. Therefore, it is very important to provide a shampoo product with plant extracts as the active ingredient, which is gentle and effective. It should be highly safe and can effectively refresh and control oil. Summary of the invention

[0005] The present invention provides a composition for shampooing with refreshing and oil-controlling effects and a preparation method thereof, wherein a plurality of plant extracts are compounded as functional components, thereby providing a highly efficient refreshing and oil-controlling effect while retaining the mildness of the plant extracts.

[0006] The invention provides a composition for shampooing with refreshing and oil-controlling effects, comprising the following components: ANEMARRHENA ASPHODELOIDES root extract, HAMAMELIS VIRGINIANA extract, MENTHA HAPLOCALYX extract, and TUBERMAGNATUM extract.

[0007] Preferably, a composition for shampooing with refreshing and oil-controlling effects comprises the following components by weight:

[0008] 10-55 parts of Anemarrhena asphodeloides root extract,

[0009] 15-80 parts of Hamamelis Virginiana extract,

[0010] 0.1-20 parts of Mentha HAPLOCALYX extract, and

[0011] 0.5~30 parts of white truffle (TUBER MAGNATUM) extract.

[0012] Preferably, a composition for shampooing with refreshing and oil-controlling effects comprises the following components by weight:

[0013] 20-35 parts of Anemarrhena Asphodeloides root extract,

[0014] 35-60 parts of Hamamelis Virginiana extract,

[0015] 8-16 parts of Mentha HAPLOCALYX extract, and

[0016] 13-28 parts of white truffle (TUBER MAGNATUM) extract.

[0017] The present invention provides application of the composition for shampooing with refreshing and oil-controlling effects in preparing hair products.

[0018] Preferably, the composition for shampooing with refreshing and oil-controlling effects is used in the preparation of hair products, and the hair products are selected from shampoo, shampoo cream, conditioner and hair mask.

[0019] Preferably, the composition for shampooing with refreshing oil-control effects is used in the preparation of hair products, and the mass percentage of the composition for shampooing with refreshing oil-control effects in the hair products is 8-15%.

[0020] The invention provides a shampoo product, comprising the composition for shampooing hair with refreshing and oil-controlling effects.

[0021] Preferably, a shampoo product contains the following components by mass percentage:

[0022] 8-15% of a composition for shampooing with refreshing and oil-control effects;

[0023] Thickener 1~15%;

[0024] Moisturizer 10~30%;

[0025] Preservatives 0.01~0.1%;

[0026] pH adjuster 0.01~3.0%;

[0027] Flavors and fragrances 0~0.5%; and

[0028] Deionized water balance.

[0029] Preferably, the thickener can be selected from one or more of hydroxypropyl methylcellulose, carbomer, xanthan gum, stearyl alcohol, cetyl alcohol, and polyurethane-10.

[0030] Preferably, the moisturizing agent can be selected from one or more of butylene glycol, octanediol, 1,3-propylene glycol, glycerol, 1,2-hexanediol, 1,2-pentanediol, glycerol, caprylyl glycol, α-glucan oligosaccharide, and allantoin.

[0031] Preferably, the preservative can be selected from one or more of phenoxyethanol, potassium sorbate, sodium benzoate, DMDM ​​hydantoin, methylisothiazolinone and methylparaben.

[0032] Preferably, the pH adjuster can be selected from one or more of citric acid, sodium citrate, tartaric acid, hydrochloric acid and sodium acetate.

[0033] Preferably, the flavor and fragrance can be selected from one or more of limonene, hexyl cinnamaldehyde, geraniol and linalool.

[0034] The invention provides a preparation method of a composition for shampooing with refreshing and oil-controlling effects, comprising the following steps: accurately weighing an extract of Anemarrhena asphodeloides root, an extract of Hamamelisi Virginiana, an extract of Mentha haplocalyx, and an extract of white truffle according to weight, and mixing them evenly to obtain the composition.

[0035] The present invention provides a method for preparing a shampoo product, comprising the following steps:

[0036] 1) Heat the surfactant and thickener to 80-90°C and stir for 40-60 minutes to mix evenly. Keep warm for 20-30 minutes and then cool to 30-45°C to obtain mixture A.

[0037] 2) Mix the refreshing oil-controlling composition for shampooing, moisturizer and deionized water, heat to 30-50° C., stir for 20-45 minutes, and mix well to obtain a mixture B;

[0038] 3) Evenly mix the mixture A obtained in step 1), the mixture B obtained in step 2), the pH adjuster, the preservative and the flavors and fragrances to obtain the product.

[0039] The beneficial technical effects of the present invention are:

[0040] In order to achieve an efficient refreshing oil control effect, the present invention selects ANEMARRHENA ASPHODELOIDES root extract, HAMAMELIS VIRGINIANA extract, MENTHA HAPLOCALYX extract, and TUBERMAGNATUM extract from a variety of plant extracts through repeated experiments. The four plant extracts are rationally combined to achieve a good balance between efficient oil control and safety and mildness, overcoming the shortcomings of the existing shampoo with plant extracts as the effective ingredient, which is not effective, and has achieved significant progress. BRIEF DESCRIPTION OF THE DRAWINGS

[0041] Figure 1 This is a statistical chart of the expression levels of PPARγ and PLIN2, genes related to lipid synthesis in sebaceous gland cells. DETAILED DESCRIPTION

[0042] In order to more fully understand the technical content of the present invention, the present invention will be further introduced and illustrated in conjunction with specific embodiments below; obviously, the embodiments described below are only part of the embodiments of the present invention, rather than all of the embodiments; based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without making creative work are within the scope of protection of the present invention.

[0043] Unless otherwise specified, the components involved in the present invention are all conventional commercially available products.

[0044] In a specific embodiment of the present invention, the Anemarrhena ASPHODELOIDES root extract is abbreviated as Anemarrhena root extract; the Hamamelisi Virginiana extract is abbreviated as Hamamelisi Virginiana extract; the Mentha HAPLOCALYX extract is abbreviated as Mentha HAPLOCALYX extract; and the white truffle (TUBER MAGNATUM) extract is abbreviated as white truffle extract.

[0045] Table 1 Examples 1-4 Compositions for shampooing with refreshing and oil-controlling effects (by weight)

[0046]

[0047] Comparative Examples 1-5 are basically the same as Example 2, except for the ratio of the plant extracts in the composition with refreshing and oil-controlling effects for shampooing, as shown in Table 2.

[0048] Table 2 Comparative Examples 1-5 Compositions for shampooing with refreshing and oil-controlling effects (by weight)

[0049]

[0050] Comparative Example 6

[0051] Comparative Example 6 is substantially the same as Example 3, except that an equal amount of Pueraria lobata extract is used instead of the white truffle extract.

[0052] Comparative Example 7

[0053] Comparative Example 7 is substantially the same as Example 3, except that an equal amount of Calendula officinalis extract is used to replace Hamamelis virginiana extract.

[0054] Comparative Example 8

[0055] Comparative Example 8 is substantially the same as Example 3, except that an equal amount of aloe vera extract is used instead of peppermint extract.

[0056] Test 1

[0057] The composition prepared in Examples 1-4 was evaluated for safety. The test method was tested with reference to the human skin patch test method in the "Technical Specification for Safety of Cosmetics (2015 Edition)". 45 healthy female testers aged 20-35 were recruited, and the screening of the testers met the inclusion and exclusion standards of the "Specifications". The composition with refreshing oil control effect for shampooing in Examples 1-4 was prepared into an 8% m / v solution with deionized water, and deionized water was set as a blank control group. The solution or deionized water of Examples 1-4 was placed in the small chamber of the patch tester, respectively, with an amount of about 0.02 mL. The patch tester with the sample was applied to the curved side of the left forearm of the tester, and lightly pressed with the palm of the hand to apply it evenly on the skin for 24 hours. After removing the test patch tester, observe the skin reaction according to the standards in Table 3 30 minutes, 24 hours, and 48 hours, and record the results.

[0058] Table 3 Grading criteria for adverse skin reactions

[0059] Degree of reaction Rating scale Skin reactions － 0 Negative reaction ± 1 Suspicious reaction, only slight erythema + 2 Weak positive reaction (erythema reaction); erythema, infiltration, edema, and papules may be present ++ 3 Strong positive reaction (herpes reaction); erythema, infiltration, edema, papules, herpes reaction beyond the test area +++ 4 Very strong positive reaction (confluent herpes reaction); obvious erythema, severe infiltration, edema, confluent herpes; reaction beyond the test area

[0060] The results showed that after 30 minutes, 24 hours and 48 hours of removing the spot tester, the test subjects of the blank control group and Example 1-4 groups all had negative reactions, indicating that the composition for shampooing with refreshing and oil-controlling effects of the present invention will not cause adverse reactions to the skin and is safe and non-irritating.

[0061] Test 2

[0062] The purchased human immortalized sebaceous gland cells SZ95 were cultured in Sebomed basal medium containing 100U / mL penicillin, 100μg / mL streptomycin, and 5ng / mL epidermal growth factor in an environment of 5% CO2 and 37°C. The experiment was performed after the subcultured cells were stable. SZ95 cells were cultured at 1*10 4 Cells were inoculated in a 96-well plate at a density of 10 cells / well, and grouped after overnight culture, with 6 replicates in each group. The wells without cells were used as blank groups. Fresh complete medium was added to the control group, 8 μM dihydrotestosterone (DHT) was added to the modeling group, and 8 μM DHT and 30 μM of the composition of Examples 1-4 and Comparative Examples 1-8 were added to the experimental group, respectively. After 36 hours of treatment, the cell proliferation was detected by the CCK8 method, and the absorbance at 450 nm was detected, with 650 nm as the reference wavelength. The results are shown in Table 4.

[0063] Table 4 SZ95 cell proliferation results (shown as average values)

[0064] A450(0h) A450(36h) Control group 0.3154 0.8966 Modeling Group 0.3241 1.2722 Example 1 0.3157 0.1006 Example 2 0.3074 0.9981 Example 3 0.3120 0.9824 Example 4 0.3175 0.9877 Comparative Example 1 0.3190 1.0914 Comparative Example 2 0.3212 1.1335 Comparative Example 3 0.3114 1.1549 Comparative Example 4 0.3135 1.1080 Comparative Example 5 0.3096 1.0876 Comparative Example 6 0.3203 1.1135 Comparative Example 7 0.3168 1.0989 Comparative Example 8 0.3142 1.1258

[0065] There was no significant difference in A450 (0h) between the control group, modeling group, Examples 1-4, and Comparative Examples 1-8, indicating that the cell seeding density of each group was uniform. There was a significant difference between the control group and the A450 (36h) of the modeling group, p<0.001, indicating that the modeling was successful. There was a significant difference between Examples 1-4 and the A450 (36h) of the modeling group, p<0.01, indicating that the composition with refreshing oil control effect for shampooing of the present invention can effectively improve DHT-induced human sebaceous gland cell proliferation. There was a significant difference between Comparative Examples 1-5 and the A450 (36h) of Example 2, p<0.05, indicating that the omission of any plant extract in the composition of the present invention or the adjustment of the dosage ratio significantly affects the inhibitory effect on human sebaceous gland cell proliferation. There are significant differences in A450 (36h) between Comparative Examples 6-8 and Example 3, p<0.05, indicating that the specific selection of the plant extract in the composition of the present invention is the key to ensuring the inhibitory effect on the proliferation of human sebaceous gland cells.

[0066] Test 3

[0067] The test method of this test was carried out with reference to the experimental method of "Effects of Clascoterone on SZ95 human sebaceous gland cell proliferation, lipid synthesis and expression of inflammatory factors, Ye Feng et al., Chinese Journal of Leprosy and Skin Diseases, Vol. 38, No. 6". The purchased human immortalized sebaceous gland cells SZ95 were cultured in a Sebomed basal medium containing 100U / mL penicillin, 100μg / mL streptomycin, and 5ng / mL epidermal growth factor in an environment of 5% CO2 and 37°C. The experiment was carried out after the subcultured cells were stable. SZ95 cells were inoculated in a culture dish and grown to a confluence of 60%. Fresh complete culture medium was added to the control group, 8μM dihydrotestosterone (DHT) was added to the modeling group, and 8μM DHT and 30μM of the composition of Examples 2-3 and Comparative Examples 1-8 were added to the experimental group, respectively. After 36 hours of treatment, the cell lysates were collected and Western blotting was used to detect the expression of genes related to sebaceous gland cell lipid synthesis. The results are shown in the figure. Figure 1 .

[0068] Lipid synthesis in sebaceous gland cells requires activation of related lipid gene expression in the cell. PPARγ is a transcription factor involved in regulating lipid metabolism. PPARγ and its target gene PLIN2 are lowly expressed in acne lesions and highly expressed in sebaceous glands of non-acne lesions, and play a protective role against excessive lipid accumulation and inflammatory response. Their activation increases lipid production in healthy sebaceous glands. The results showed that DHT induced high expression of PPARγ and PLIN2 in SZ95 cells, and the composition of the present invention can effectively reduce the expression of PPARγ and PLIN2, and the composition of Examples 2-3 of the present invention has a better inhibitory effect than Comparative Examples 1-8.

[0069] Test 4

[0070] 80 healthy volunteers with dandruff and itchy scalp were recruited, with an age range of 25 to 40 years old. The volunteers were randomly divided into 10 groups, with 8 cases in each group. The shampoos prepared from the compositions with refreshing oil control effects for shampooing of Examples 2-3 and Comparative Examples 1-8 were used respectively. The oil control effect of the shampoos was sensory evaluated. The shampoos were used 2 to 3 times a week, and the scalp was massaged with the shampoo for at least 2 minutes during washing. The shampoos were used continuously for 8 weeks. After 8 weeks, a scoring system of 1 to 10 points was used, with 1 point indicating completely ineffective and 10 points indicating very effective. The sensory evaluation results are shown in Table 6.

[0071] Table 5 Shampoo formula (wt%)

[0072] Composition for shampooing with refreshing oil-control effect Carbomer 1,2-Pentanediol Caprylyl glycol Butanediol Sodium Benzoate Citric Acid Deionized water Implementation Group 2 12 2 0.1 0.3 9 0.1 0.2 margin Implementation Group 3 12 2 0.1 0.3 9 0.1 0.2 margin Comparison group 1 12 2 0.1 0.3 9 0.1 0.2 margin Comparison group 2 12 2 0.1 0.3 9 0.1 0.2 margin Comparison group 3 12 2 0.1 0.3 9 0.1 0.2 margin Comparison group 4 12 2 0.1 0.3 9 0.1 0.2 margin Comparison group 5 12 2 0.1 0.3 9 0.1 0.2 margin Comparison group 6 12 2 0.1 0.3 9 0.1 0.2 margin Comparison group 7 12 2 0.1 0.3 9 0.1 0.2 margin Comparison group 8 12 2 0.1 0.3 9 0.1 0.2 margin

[0073] Table 6 Sensory evaluation results

[0074] Group Oil control effect Implementation Group 2 9.2 Implementation Group 3 9.4 Comparison group 1 7.9 Comparison group 2 7.4 Comparison group 3 7.6 Comparison group 4 8.2 Comparison group 5 8.1 Comparison group 6 7.8 Comparison group 7 7.7 Comparison group 8 8.0

[0075] The results show that the composition water with refreshing oil-controlling effect for shampooing of the present invention can effectively control oil and make the scalp refreshing.

[0076] The above contents are further detailed descriptions of the present invention in combination with specific preferred embodiments, and it cannot be determined that the specific implementation of the present invention is limited to these descriptions. For ordinary technicians in the technical field to which the present invention belongs, several simple deductions or substitutions can be made without departing from the concept of the present invention, which should be regarded as falling within the protection scope of the present invention.

Claims

1. A composition for shampooing with refreshing and oil-controlling effects, comprising the following components by weight: 20-35 parts of Anemarrhena Asphodeloides root extract, 35-60 parts of Hamamelis Virginiana extract, 8-16 parts of Mentha HAPLOCALYX extract, and 13-28 parts of white truffle (TUBER MAGNATUM) extract.

2. Use of the composition for shampooing with refreshing oil-control effects according to claim 1 in the preparation of hair products; the mass percentage of the composition for shampooing with refreshing oil-control effects in the hair products is 8 to 15%.

3. The use according to claim 2, characterized in that: The hair product is selected from shampoo, shampoo cream, hair conditioner and hair mask.

4. The method for preparing the composition for shampooing with refreshing and oil-controlling effects according to claim 1, comprising the following steps: accurately weighing the root extract of Anemarrhena asphodeloides, the extract of Hamamelis virginiana, the extract of Mentha haplocalyx, and the extract of white truffle according to weight, and mixing them evenly to obtain the composition.

5. A shampoo product, comprising the following components by mass percentage: 8-15% of the refreshing and oil-controlling composition for shampooing according to claim 1; Thickener 1~15%; Moisturizer 10~30%; Preservatives 0.01~0.1%; pH adjuster 0.01~3.0%; Flavors and fragrances 0~0.5%; as well as Deionized water balance.

6. The shampoo product according to claim 5, characterized in that The thickener can be selected from one or more of hydroxypropyl methylcellulose, carbomer, xanthan gum, stearyl alcohol, cetyl alcohol, and polyurethane-10; the moisturizer can be selected from one or more of butylene glycol, caprylyl glycol, 1,3-propylene glycol, glycerol, 1,2-hexanediol, 1,2-pentanediol, glycerol, caprylyl glycol, α-glucan oligosaccharide, and allantoin.

7. The shampoo product according to claim 5 or 6, characterized in that: The preservative can be selected from one or more of phenoxyethanol, potassium sorbate, sodium benzoate, DMDM ​​hydantoin, methylisothiazolinone, and methylparaben; the pH adjuster can be selected from one or more of citric acid, sodium citrate, tartaric acid, hydrochloric acid, and sodium acetate; the flavor and fragrance can be selected from one or more of limonene, hexyl cinnamaldehyde, geraniol, and linalool.

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