A whitening and freckle-removing Chinese medicine composition and preparation method thereof

Abstract

The present invention relates to a whitening and freckle-removing Chinese medicine composition and a preparation method thereof, and specifically discloses a whitening and freckle-removing Chinese medicine composition, which comprises, by weight, 0.5-3 parts of ophiopogon japonicus extract, 0.1-2 parts of licorice root extract, 0.05-1 parts of ebony extract, 0.1-1 parts of rhodiola rosea extract, 0.1-1 parts of safflower extract, 0.1-1 parts of silybum marianum extract, 0.5-2 parts of prickle fruit extract, 0.1-2 parts of glycerate, 10-38 parts of polyols, 0.05-0.5 parts of carboxymethyl chitosan and 30-100 parts of water. The whitening and freckle-removing composition prepared by compounding carboxymethyl chitosan and polyols with Chinese medicine extracts can act on melanocytes to inhibit the synthesis of melanin, while inhibiting inflammatory response, repairing skin barrier, promoting skin microcirculation, and then realizing whitening and freckle removal from multiple dimensions.

Description

Technical Field

[0001] The invention belongs to the technical field of cosmetics, and particularly relates to a whitening and freckle-removing traditional Chinese medicine composition and a preparation method thereof. Background Art

[0002] With the development of social economy, the pressure of life is increasing day by day. Modern urban people have entered a fast-paced life with irregular diet and work and rest, such as staying up late for a long time, lack of sleep, etc., coupled with the current situation of ultraviolet radiation and long-term exposure to radiation from electronic screens such as mobile phones and computers. It is easy for modern women's skin to become bumpy and dull, which in turn leads to more and more adverse symptoms such as dark yellow skin, pigmentation, and spots.

[0003] Chinese herbal cosmetics have a long history. Chinese medicine has a history of thousands of years in my country and has long been accepted by people. However, the pigmentation of the skin is affected by many factors. It is difficult for a single Chinese herbal raw material to achieve multi-dimensional freckle removal and whitening effects. Excessive compounding of Chinese herbal raw materials will lead to the problem of unstable compounding of raw materials and difficulty in controlling transdermal absorption.

[0004] In the prior art, CN108186456B discloses a method for preparing an extract of Phyllanthus emblica with freckle removal and whitening activity. The chemical composition of the extract is mainly total tannin, and the total flavonoid content of the impurity component is less than 5%. The extract has significant freckle removal and whitening activity and no skin irritation after evaluation by a melanin inhibition test. CN104784475B discloses a Chinese medicine formula extract with whitening and freckle removal effects. The Chinese medicine formula extract of the invention is made of ten kinds of Chinese medicines such as radix aconitifolii, radix scoparia, persica, calamus, bilberry, raspberry, tuckahoe, polygala, polygonatum, and ginseng as raw materials, and the active ingredients are extracted by using carbon dioxide supercritical extraction technology and macroporous adsorption resin enrichment technology. The prepared Chinese medicine formula extract has significant tyrosinase activity inhibition and melanin production inhibition. Summary of the invention

[0005] In order to solve the above technical problems, the present invention discloses a whitening and freckle-removing Chinese medicine composition and a preparation method thereof. Specifically, the present invention acts on melanocytes to simultaneously inhibit inflammatory responses, repair skin barriers, and promote skin microcirculation, thereby achieving multi-dimensional whitening and freckle removal.

[0006] In order to achieve the above object, the present invention adopts the following technical solutions:

[0007] A whitening and freckle-removing Chinese medicine composition, comprising the following components by weight:

[0008] The invention comprises 0.5-3 parts of ophiopogon japonicus extract, 0.1-2 parts of licorice root extract, 0.05-1 parts of ebony extract, 0.1-1 parts of rhodiola rosea extract, 0.1-1 parts of safflower extract, 0.1-1 parts of milk thistle extract, 0.5-2 parts of prickle fruit extract, 10-40 parts of stabilizer and 30-100 parts of water.

[0009] The ophiopogon japonicus extract is ophiopogon japonicus polysaccharide.

[0010] Preferably, the ratio of Ophiopogon japonicus extract: Glycyrrhiza glabra root extract: Prunus mume extract: Rhodiola rosea extract: Carthamus tinctorius extract: Silybum marianum extract: Prinus prilocaena extract is 2:1:0.5:0.5:0.5:0.5:1.

[0011] The stabilizers are glycerate, polyol and carboxymethyl chitosan.

[0012] Preferably, the glyceride is one or more of monocaprylic glyceryl or quinic glyceryl;

[0013] More preferably, the glyceride is glyceryl monocaprylate.

[0014] The polyol is one or more of propylene glycol, butylene glycol and hexylene glycol.

[0015] Further preferred polyols are propylene glycol and butylene glycol.

[0016] The substitution degree of the carboxymethyl chitosan is between 0.3 and 0.5, and the molecular weight is between 600 KDa and 1000 KDa.

[0017] The stabilizer comprises 0.1-2 parts of glycerate, 10-38 parts of polyol and 0.05-0.5 parts of carboxymethyl chitosan according to the mass fraction of the composition.

[0018] The present invention also provides a method for preparing a whitening and freckle-removing Chinese medicine composition, comprising the following steps:

[0019] S1. Dissolve the Ophiopogon japonicus extract and carboxymethyl chitosan in part of water to obtain a mixture A for later use.

[0020] S3: dissolving the Glycyrrhiza glabra root extract, Prunus mume extract, Rhodiola rosea extract and Carthamus tinctorius extract in polyol to obtain a mixture B for later use.

[0021] S3: Mix the marian thistle extract, the prickle fruit extract and glyceride to obtain a mixture C for later use.

[0022] S4: Add mixture C to mixture B, then add the remaining amount of water, mix well, and then add to mixture A for high-pressure homogenization to obtain a whitening and freckle-removing combination.

[0023] The mixing is low-temperature stirring, and the stirring temperature is ≤60°C; the high-pressure homogenization pressure is 80-120bar, and the number of cycles is 3-5 times.

[0024] Preferably, the low-temperature stirring has a stirring temperature of 45°C.

[0025] Preferably, the high-pressure homogenization pressure is 100 bar.

[0026] Beneficial effects:

[0027] 1. The whitening and freckle-removing Chinese medicinal composition disclosed in the present invention has the effects of inhibiting skin inflammation and repairing the skin, and can also inhibit the activity of tyrosinase and the synthesis of melanin by melanocytes, thereby exerting the effects of whitening and freckle-removing.

[0028] 2. The whitening and freckle-removing Chinese herbal medicine composition disclosed in the present invention is compounded with a variety of Chinese herbal extracts, which can achieve synergistic effects between the various effective extracts, thereby achieving excellent whitening and freckle-removing effects.

[0029] 3. The whitening and freckle-removing Chinese medicinal composition disclosed in the present invention improves the storage stability of the composition by compounding polyols with carboxymethyl chitosan. Specific embodiments

[0030] The technical scheme of the present invention is further described below in conjunction with specific examples. The raw materials used in the following examples, unless otherwise specified, can be obtained from conventional commercial sources; the processes used, unless otherwise specified, can all be conventional processes in the art.

[0031] Table 1 Raw material information

[0032] raw material supplier Ophiopogon japonicus extract Shandong Tianjiu Biotechnology Co., Ltd. Licorice Root Extract Xi'an Yatu Biotechnology Co., Ltd. Black plum extract Baoji Kaiweikang Biotechnology Co., Ltd. Rhodiola Rosea Extract Shaanxi Benhe Bioengineering Co., Ltd. Safflower Extract Shanghai Guanyang Food Technology Co., Ltd. Milk Thistle Extract Xi'an Dongfeng Biotechnology Co., Ltd. Prinus prickle fruit extract Shanxi Guopeptide Biotechnology Co., Ltd.

[0033] Example 1

[0034] Preparation method of whitening and freckle-removing Chinese medicine composition

[0035] S1. According to the weight percentage, 2 parts of Ophiopogon japonicus extract and 0.3 parts of carboxymethyl chitosan are dissolved in water accounting for 60% of the total water consumption to obtain a mixture A for later use.

[0036] S2: Dissolve 1 part of Glycyrrhiza glabra root extract, 0.5 part of Prunus mume extract, 0.5 part of Rhodiola rosea extract and 0.5 part of Carthamus tinctorius extract in 15 parts of 1,3-propylene glycol and 10 parts of 1,4-butylene glycol to obtain a mixture B for later use.

[0037] S3: 0.5 parts of thistle extract, 1 part of Primula prickle extract, and 0.5 parts of monocaprylin are mixed to obtain a mixture C for later use.

[0038] S4: Add mixture C to mixture B, add water to 100 parts, stir and mix evenly at 45°C, then add to mixture A and perform high pressure homogenization at 100 bar for 3 cycles to obtain a whitening and anti-freckle combination.

[0039] Example 2

[0040] Preparation method of whitening and freckle-removing Chinese medicine composition

[0041] S1. According to the weight percentage, 0.5 parts of Ophiopogon japonicus extract and 0.05 parts of carboxymethyl chitosan are dissolved in 60% of the total water to obtain a mixture A for later use.

[0042] S2: Dissolve 0.1 parts of Glycyrrhiza glabra root extract, 0.05 parts of Prunus mume extract, 0.1 parts of Rhodiola rosea extract and 0.1 parts of Carthamus tinctorius extract in 10 parts of 1,3-propylene glycol to obtain a mixture B for later use.

[0043] S3: 0.1 parts of thistle extract, 0.5 parts of Primula prickle extract and 0.1 parts of monocaprylin are mixed to obtain a mixture C for later use.

[0044] S4: Add mixture C to mixture B, add water to 100 parts, stir and mix at 45°C, then add to mixture A and perform high pressure homogenization at 80 bar for 3 cycles to obtain a whitening and freckle removal combination.

[0045] Example 3

[0046] Preparation method of whitening and freckle-removing Chinese medicine composition

[0047] S1. According to the weight percentage, 3 parts of Ophiopogon japonicus extract and 0.5 parts of carboxymethyl chitosan are dissolved in water accounting for 60% of the total water consumption to obtain a mixture A for later use.

[0048] S2: Dissolve 2 parts of Glycyrrhiza glabra root extract, 1 part of Prunus mume extract, 1 part of Rhodiola rosea extract and 1 part of Carthamus tinctorius extract in 20 parts of 1,3-propylene glycol and 18 parts of 1,4-butylene glycol to obtain a mixture B for later use.

[0049] S3: Mix 1 part of thistle extract, 2 parts of Principis prinus extract and 2 parts of monocaprylin to obtain a mixture C for later use.

[0050] S4: Add mixture C to mixture B, add water to 100 parts, stir and mix evenly at 45°C, then add to mixture A and perform high pressure homogenization at 120 bar for 5 cycles to obtain a whitening and freckle removal combination.

[0051] Comparative Example 1

[0052] Preparation method of whitening and freckle-removing Chinese medicine composition

[0053] S1. According to the mass fraction, 0.3 parts of carboxymethyl chitosan is dissolved in 60% of the total water to obtain a mixture A for standby use.

[0054] S2: Dissolve 1 part of Glycyrrhiza glabra root extract, 0.5 part of Prunus mume extract, 0.5 part of Rhodiola rosea extract and 0.5 part of Carthamus tinctorius extract in 15 parts of 1,3-propylene glycol and 10 parts of 1,4-butylene glycol to obtain a mixture B for later use.

[0055] S3: 0.5 parts of thistle extract, 1 part of Primula prickle extract, and 0.5 parts of monocaprylin are mixed to obtain a mixture C for later use.

[0056] S4: Add mixture C to mixture B, add water to 100 parts, stir and mix evenly at 45°C, then add to mixture A and perform high pressure homogenization at 100 bar for 3 cycles to obtain a whitening and anti-freckle combination.

[0057] Comparative Example 2

[0058] Preparation method of whitening and freckle-removing Chinese medicine composition

[0059] S1. According to the weight percentage, 2 parts of Ophiopogon japonicus extract and 0.3 parts of carboxymethyl chitosan are dissolved in water accounting for 60% of the total water consumption to obtain a mixture A for later use.

[0060] S2: Dissolve 1 part of Glycyrrhiza glabra root extract, 0.5 parts of Rhodiola rosea extract, and 0.5 parts of Carthamus tinctorius extract in 15 parts of 1,3-propylene glycol and 10 parts of 1,4-butylene glycol to obtain a mixture B for later use.

[0061] S3: 0.5 parts of thistle extract, 1 part of Primula prickle extract, and 0.5 parts of monocaprylin are mixed to obtain a mixture C for later use.

[0062] S4: Add mixture C to mixture B, add water to 100 parts, stir and mix evenly at 45°C, then add to mixture A and perform high pressure homogenization at 100 bar for 3 cycles to obtain a whitening and anti-freckle combination.

[0063] Comparative Example 3

[0064] Preparation method of whitening and freckle-removing Chinese medicine composition

[0065] S1. According to the weight percentage, 2 parts of Ophiopogon japonicus extract and 0.3 parts of carboxymethyl chitosan are dissolved in water accounting for 60% of the total water consumption to obtain a mixture A for later use.

[0066] S2: Dissolve 1 part of Glycyrrhiza glabra root extract, 0.5 parts of Prunus mume extract and 0.5 parts of Carthamus tinctorius extract in 15 parts of 1,3-propylene glycol and 10 parts of 1,4-butylene glycol to obtain a mixture B for later use.

[0067] S3: 0.5 parts of thistle extract, 1 part of Primula prickle extract, and 0.5 parts of monocaprylin are mixed to obtain a mixture C for later use.

[0068] S4: Add mixture C to mixture B, add water to 100 parts, stir and mix evenly at 45°C, then add to mixture A and perform high pressure homogenization at 100 bar for 3 cycles to obtain a whitening and anti-freckle combination.

[0069] Comparative Example 4

[0070] Preparation method of whitening and freckle-removing Chinese medicine composition

[0071] S1. According to the weight percentage, 2 parts of Ophiopogon japonicus extract and 0.3 parts of carboxymethyl chitosan are dissolved in water accounting for 60% of the total water consumption to obtain a mixture A for later use.

[0072] S2: Dissolve 1 part of Glycyrrhiza glabra root extract, 0.5 parts of Prunus mume extract and 0.5 parts of Rhodiola rosea extract in 15 parts of 1,3-propylene glycol and 10 parts of 1,4-butylene glycol to obtain a mixture B for later use.

[0073] S3: 0.5 parts of thistle extract, 1 part of Primula prickle extract, and 0.5 parts of monocaprylin are mixed to obtain a mixture C for later use.

[0074] S4: Add mixture C to mixture B, add water to 100 parts, stir and mix evenly at 45°C, then add to mixture A and perform high pressure homogenization at 100 bar for 3 cycles to obtain a whitening and anti-freckle combination.

[0075] Comparative Example 5

[0076] Preparation method of whitening and freckle-removing Chinese medicine composition

[0077] S1. According to the weight percentage, 2 parts of Ophiopogon japonicus extract and 0.3 parts of carboxymethyl chitosan are dissolved in water accounting for 60% of the total water consumption to obtain a mixture A for later use.

[0078] S2: Dissolve 1 part of Glycyrrhiza glabra root extract, 0.5 part of Prunus mume extract, 0.5 part of Rhodiola rosea extract and 0.5 part of Carthamus tinctorius extract in 15 parts of 1,3-propylene glycol and 10 parts of 1,4-butylene glycol to obtain a mixture B for later use.

[0079] S3: Mix 1 part of Prinus prickle extract and 0.5 part of monocaprylin to obtain a mixture C for later use.

[0080] S4: Add mixture C to mixture B, add water to 100 parts, stir and mix evenly at 45°C, then add to mixture A and perform high pressure homogenization at 100 bar for 3 cycles to obtain a whitening and anti-freckle combination.

[0081] Comparative Example 6

[0082] Preparation method of whitening and freckle-removing Chinese medicine composition

[0083] S1. According to the weight percentage, 2 parts of Ophiopogon japonicus extract and 0.3 parts of carboxymethyl chitosan are dissolved in water accounting for 60% of the total water consumption to obtain a mixture A for later use.

[0084] S2: Dissolve 1 part of Glycyrrhiza glabra root extract, 0.5 part of Prunus mume extract, 0.5 part of Rhodiola rosea extract and 0.5 part of Carthamus tinctorius extract in 15 parts of 1,3-propylene glycol and 10 parts of 1,4-butylene glycol to obtain a mixture B for later use.

[0085] S3: 0.5 parts of marian extract and 0.5 parts of monocaprylin are mixed to obtain a mixture C for later use.

[0086] S4: Add mixture C to mixture B, add water to 100 parts, stir and mix evenly at 45°C, then add to mixture A and perform high pressure homogenization at 100 bar for 3 cycles to obtain a whitening and anti-freckle combination.

[0087] Comparative Example 7

[0088] Preparation method of whitening and freckle-removing Chinese medicine composition

[0089] S1. Dissolve 2 parts of Ophiopogon japonicus extract in 60% of the total water to obtain a mixture A for later use.

[0090] S2: Dissolve 1 part of Glycyrrhiza glabra root extract, 0.5 part of Prunus mume extract, 0.5 part of Rhodiola rosea extract and 0.5 part of Carthamus tinctorius extract in 15 parts of 1,3-propylene glycol and 10 parts of 1,4-butylene glycol to obtain a mixture B for later use.

[0091] S3: 0.5 parts of thistle extract, 1 part of Primula prickle extract, and 0.5 parts of monocaprylin are mixed to obtain a mixture C for later use.

[0092] S4: Add mixture C to mixture B, add water to 100 parts, stir and mix evenly at 45°C, then add to mixture A and perform high pressure homogenization at 100 bar for 3 cycles to obtain a whitening and anti-freckle combination.

[0093] Efficacy evaluation

[0094] 1. DPPH free radical scavenging rate detection

[0095] Method: Prepare 0.05g / mL DPPH reagent (dissolved in anhydrous ethanol) and keep its absorbance at 519nm between 0.7 and 0.9. Add 50μL of the sample to be tested (1% water dilution of the whitening and freckle-removing composition) and 100μL of DPPH solution into a 96-well plate, and set a control well (containing 50μL of anhydrous ethanol). After reacting at 37℃ for 30min, detect the absorbance at 519nm.

[0096] Table 2 DPPH free radical scavenging rate

[0097]

[0098]

[0099] As shown in Table 2, compared with Examples 1-3, it can be seen that the amount of the whitening and freckle removal composition greatly affects the antioxidant effect of the composition. Comparing Example 1 with Comparative Examples 1-7, it can be seen that the whitening and freckle removal composition in Example 1 has the best antioxidant effect.

[0100] 2. Cellular Anti-inflammatory Experiment

[0101] RAW264.7 were inoculated into 24-well plates (1×104 cells / well). On the second day, different test substances (1% water dilution of the whitening and anti-freckle composition) were added to the cells for 1 hour in advance, and then 1 μg / mL of the inducer LPS was added. A control group (without the test substance) was set up. After the two substances were acted together for 24 hours, the cell supernatant was collected and the operation was carried out according to the instructions of the TNFα ELISA detection kit.

[0102] Table 3 TNFα content

[0103]

[0104]

[0105] 3. Secretion of melanin

[0106] B16F10 cells were seeded in 6-well plates (1×10 5 / well), and on the next day, different test substances ((whitening and freckle removal composition 1% water dilution)) were added to act on the cells for 1 hour in advance, and then 1μmol / L of the inducer α-MSH was added, and a control group (containing only α-MSH) was set up. After the two substances acted together for 48 hours, the cells were collected for subsequent measurements, and the final results were calibrated with cell counts.

[0107] Melanin content: Collect the cell pellet from each well and add 0.4 mL of 1 mol / L NaOH solution (containing 10% DMSO by volume), incubate at 80° C. for 30 min, and detect the absorbance at 490 nm.

[0108] Table 4 Melanin content

[0109]

[0110]

[0111] 4. Cell Repair

[0112] BJ cells were inoculated into 24-well plates (1×104 cells / well). The cells were scratched the next day and different test substances ((1% water dilution of the whitening and freckle removal composition)) were added. A control group (without the test substance) was set up. After the two substances acted together for 24 hours, the scratch repair rate was tested.

[0113] Scratch repair rate = (T0 scratch width - T24 scratch width) / T0 scratch width * 100%

[0114] Table 5 Scratch repair rate

[0115]

[0116]

[0117] 5. Stability Observation

[0118] Referring to the requirements of the fourth guideline 9001 "Guidelines for Stability Testing of APIs and Pharmaceutical Preparations" of the 2020 edition of the "Chinese Pharmacopoeia", the above-mentioned composition was placed at a temperature of 45±2°C, a relative humidity of 75±5% and a temperature of 25±2°C, a relative humidity of 60±5% and observed for color changes for 0-6 months.

[0119] Table 6 State changes of the whitening and freckle removal composition under different environmental conditions

[0120]

[0121]

[0122] The above embodiments are preferred implementation modes of the present invention, but the implementation modes of the present invention are not limited to the above embodiments. Any other changes, modifications, substitutions, combinations, and simplifications that do not deviate from the spirit and principles of the present invention should be equivalent replacement methods and are included in the protection scope of the present invention.

Claims

1. A whitening and freckle-removing Chinese medicine composition, characterized in that: The whitening and freckle-removing Chinese medicinal composition is composed, by weight, of 0.5-3 parts of ophiopogon japonicus extract, 0.1-2 parts of licorice root extract, 0.05-1 parts of ebony extract, 0.1-1 parts of rhodiola rosea extract, 0.1-1 parts of safflower extract, 0.1-1 parts of milk thistle extract, 0.5-2 parts of prickle fruit extract, 10-40 parts of stabilizer and 30-100 parts of water.

2. The whitening and freckle-removing Chinese medicine composition according to claim 1, characterized in that: The ophiopogon japonicus extract is ophiopogon japonicus polysaccharide.

3. The whitening and freckle-removing Chinese medicine composition according to claim 2, characterized in that: The stabilizer is glycerate, polyol and carboxymethyl chitosan.

4. The whitening and freckle-removing Chinese medicinal composition according to claim 3, characterized in that: The polyol is one or more of propylene glycol, butylene glycol and hexylene glycol.

5. The whitening and freckle-removing Chinese medicinal composition according to claim 4, characterized in that: The glyceride is one or more of monocaprylic glyceride or quinic glyceride.

6. The whitening and freckle-removing Chinese medicinal composition according to claim 5, characterized in that: The substitution degree of the carboxymethyl chitosan is between 0.3 and 0.5, and the molecular weight is between 600 KDa and 1000 KDa.

7. The whitening and freckle-removing Chinese medicinal composition according to claim 6, characterized in that: The stabilizer is composed of 0.1-2 parts of glycerate, 10-38 parts of polyol and 0.05-0.5 parts of carboxymethyl chitosan according to the mass fraction of the composition.

8. A method for preparing the whitening and freckle-removing Chinese medicinal composition according to any one of claims 3 to 7, characterized in that: The following steps are involved: S1, dissolving the Ophiopogon japonicus extract and carboxymethyl chitosan in part of water to obtain a mixture A for later use; S2: dissolving the extract of Glycyrrhiza glabra root, Prunus mume extract, Rhodiola rosea extract and Carthamus tinctorius extract in polyol to obtain a mixture B for later use; S3: mixing the milk thistle extract, the prickle fruit extract and the glyceride to obtain a mixture C for later use; S4: Add mixture C to mixture B, then add the remaining amount of water, mix well, and then add to mixture A for high-pressure homogenization to obtain a whitening and freckle-removing Chinese medicine composition.

9. The preparation method according to claim 8, characterized in that: The mixing is low-temperature stirring, and the stirring temperature is ≤60°C; the high-pressure homogenization pressure is 100 bar, and the number of cycles is 3-5 times.

10. Use of the whitening and freckle-removing traditional Chinese medicine composition according to any one of claims 1 to 7 in the preparation of whitening or freckle-removing cosmetics.

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