A method for domesticating wild seeds of Limonium truncatum

Abstract

The invention provides a method for domesticating wild seeds of Limonium truncatum, and belongs to the technical field of wild seed domestication. The wild seeds of Limonium truncatum are sequentially soaked with a growth regulator and a potassium permanganate solution and then dried; the dried wild seeds of Limonium truncatum are sown to cultivate seedlings, and after the seedlings grow to 15 to 35 cm, nutrient solution A and fungicide are sprayed respectively, and then nutrient solution B is sprayed every 7 to 15 days, and Limonium truncatum plants are cultivated in a greenhouse; Limonium truncatum plants with excellent performance are selected and threshed to obtain Limonium truncatum seeds, and the Limonium truncatum seeds are continuously selected and cultivated for 3 to 5 times to obtain domesticated Limonium truncatum seeds. Compared with wild Limonium truncatum, the domesticated Limonium truncatum has excellent properties such as short growth period, long reproductive growth period, less seed shedding, uniform maturity, high survival rate, high yield and strong stress resistance.

Description

Technical Field

[0001] The invention belongs to the technical field of wild seed domestication, and in particular relates to a method for domesticating wild seeds of Limonium aureum. Background Art

[0002] Yellow Limonium is a perennial herb of the blue snow family, resistant to drought and salinity, and has strong vitality. It grows in arid desert conditions such as Gobi, beach, rocky hillside, semi-mobile sand dunes and fixed sand dunes. The flowers of Yellow Limonium can be used as medicine, with analgesic, anti-inflammatory, spleen-strengthening, hemostatic and other effects; the calyx of Yellow Limonium is membranous and persistent, not easy to fall off, and has a long retention time, and has a high ornamental value, and is an ideal material for dried flowers and flower arrangements. Therefore, Yellow Limonium is also a common and excellent ornamental flower in arid desert areas. The outstanding feature of this plant is that it is extremely resistant to drought and salinity. Its wild habitat is mainly arid and barren deserts or Gobi deserts, as well as some meadows and coastal areas with high salinity. It has a strong adaptability to harsh environments, but the wild seeds of Yellow Limonium are inconsistent in maturity, have strong grain shedding, have a long growth period, and have poor production performance, which is not conducive to widespread planting. Therefore, it is urgent to develop a domestication method for wild seeds of Yellow Limonium to provide technical support for the development and utilization of this plant resource. Summary of the invention

[0003] In view of this, the object of the present invention is to provide a method for domesticating wild seeds of Limonium yellowii to solve the problems of inconsistent maturity, strong grain shedding, long growth period and poor production performance of wild seeds of Limonium yellowii.

[0004] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:

[0005] The present invention provides a method for domesticating wild seeds of Limonium candidum, comprising the following steps:

[0006] 1) soaking wild seeds of Limonium candidum in a growth regulator and potassium permanganate solution in sequence and then drying them;

[0007] 2) sowing the dried wild seeds of Limonium sibiricum to obtain seedlings, spraying the seedlings with nutrient solution A and fungicide after they grow to 15 to 35 cm, and then spraying them with nutrient solution B every 7 to 15 days to obtain Limonium sibiricum plants in a greenhouse;

[0008] 3) Selecting Limonium candidum plants with excellent performance and threshing them to obtain Limonium candidum seeds, and continuously breeding and cultivating the Limonium candidum seeds for 3 to 5 times according to steps 1) to 2) to obtain domesticated Limonium candidum seeds.

[0009] Preferably, the growth regulator is 2-5 mg / L gibberellin and 100-200 mg / L indoleacetic acid; the soaking time of the growth regulator is 18-30 hours.

[0010] Preferably, the concentration of the potassium permanganate solution is 0.1-0.3%, and the soaking time of the potassium permanganate solution is 1-3 hours.

[0011] Preferably, the sowing matrix in step 2) is composed of the following ingredients in parts by weight: 15-25 parts of peat soil, 15-25 parts of perlite, 5-15 parts of borax, 5-15 parts of sandy loam and 2-8 parts of sterilized humus soil; the moisture content of the sowing matrix is ​​10-15%.

[0012] Preferably, the sowing density in step 2) is 2 to 5 seeds / cm 2 ; The sowing depth is 0.5 to 1 cm.

[0013] Preferably, the temperature of the greenhouse culture in step 2) is 15-25° C., and the lighting time of the greenhouse culture is 17-19 h / d.

[0014] Preferably, the nutrient solution A in step 2) uses water as a solvent and includes the following components in concentrations: 2-5 g / L amino acids, 0.5-2 g / L zinc sulfate, 1-3 g / L brassinolide, 5-15 mg / L diethylaminoethyl, 0.5-2 g / L rhamnolipid, and 25-50 mg / L gibberellin; the spraying amount of the nutrient solution A is 200-400 mL / m 2 .

[0015] Preferably, the fungicide in step 2) uses water as solvent and includes the following components in concentrations: 0.5-2 g / L of root rot, 0.5-2 g / L of carbendazim; the spraying amount of the fungicide is 100-300 mL / m 2 .

[0016] Preferably, the nutrient solution B is composed of the following components by mass fraction: 0.2-1% sodium nitrophenolate, 0.01-0.03% triacontanol, 0.1-0.3% alkylphenol polyoxyethylene ether and the balance water; the spraying amount of the nutrient solution B is 100-200mL / m 2 .

[0017] Compared with the prior art, the present invention has the following beneficial effects:

[0018] The present invention provides a method for domesticating wild seeds of Limonium truncatum. Compared with wild Limonium truncatum, the Limonium truncatum domesticated by the method not only inherits the characteristics of wild materials such as drought resistance, cold resistance and salt-alkali resistance, but also has excellent properties such as short growth period, long reproductive growth period, less seed shedding, uniform maturity, high survival rate and high yield. DETAILED DESCRIPTION

[0019] The present invention provides a method for domesticating wild seeds of Limonium candidum, comprising the following steps:

[0020] 1) soaking wild seeds of Limonium candidum in a growth regulator and potassium permanganate solution in sequence and then drying them;

[0021] 2) sowing the dried wild seeds of Limonium sibiricum to obtain seedlings, spraying the seedlings with nutrient solution A and fungicide after they grow to 15 to 35 cm, and then spraying them with nutrient solution B every 7 to 15 days to obtain Limonium sibiricum plants in a greenhouse;

[0022] 3) Selecting Limonium candidum plants with excellent performance and threshing them to obtain Limonium candidum seeds, and continuously breeding and cultivating the Limonium candidum seeds for 3 to 5 times according to steps 1) to 2) to obtain domesticated Limonium candidum seeds.

[0023] In the present invention, the wild seeds of Limonium fulgens are soaked in a growth regulator and a potassium permanganate solution in sequence and then dried. The growth regulator is preferably 2-5 mg / L of gibberellin and 100-200 mg / L of indoleacetic acid; the concentration of the gibberellin is preferably 3-4 mg / L, and the concentration of the indoleacetic acid is preferably 120-180 mg / L; the soaking time of the growth regulator is preferably 18-30 hours, and more preferably 20-28 hours; the concentration of the potassium permanganate solution is preferably 0.1-0.3%, and more preferably 0.15-0.25%; the soaking time of the potassium permanganate solution is preferably 1-3 hours, and more preferably 1.5-2.5 hours. Soaking in the growth regulator and potassium permanganate solution can reduce the hardness of wild grass seeds, break their dormancy period, and improve the germination rate of seeds while playing a disinfecting role.

[0024] In the present invention, the dried wild seeds of Limonium truncatum are sown to cultivate seedlings. The sowing matrix is ​​composed of the following components in parts by weight: 15-25 parts of peat soil, 15-25 parts of perlite, 5-15 parts of borax, 5-15 parts of sandy loam and 2-8 parts of sterilized humus; the amount of peat soil is preferably 18-22 parts; the amount of perlite is preferably 18-22 parts; the amount of borax is preferably 8-12 parts; the amount of sandy loam is preferably 8-12 parts; the amount of sterilized humus is preferably 3-6 parts; the moisture content of the sowing matrix is ​​preferably 10-15%, more preferably 11-14%; the sowing density is preferably 2-5 grains / cm 2 , more preferably 3 to 4 particles / cm 2The sowing depth is preferably 0.5-1 cm, more preferably 0.6-0.9 cm. When the seedlings grow to 15-35 cm, nutrient solution A and fungicide are sprayed respectively, and then nutrient solution B is sprayed every 7-15 days, and the yellow flower Limonium plants are cultivated in a greenhouse. The nutrient solution A uses water as a solvent and includes the following components in concentrations: 2-5 g / L of amino acids, 0.5-2 g / L of zinc sulfate, 1-3 g / L of brassinolide, 5-15 mg / L of amikacin, 0.5-2 g / L of rhamnolipid, and 25-50 mg / L of gibberellin; the amount of the amino acids is preferably 3-4 g / L; the amount of the zinc sulfate is preferably 0.8-1.8 g / L; the amount of brassinolide is preferably 1.5-2.5 g / L; the amount of amikacin is preferably 8-12 mg / L; the amount of rhamnolipid is preferably 0.8-1.5 g / L; the amount of gibberellin is preferably 30-45 mg / L; the spraying amount of the nutrient solution A is preferably 200-400 mL / m 2 , more preferably 250 to 350 mL / m 2 The fungicide uses water as solvent and includes the following components in concentrations: 0.5-2 g / L of rutin and 0.5-2 g / L of carbendazim; the dosage of rutin is preferably 0.8-1.8 g / L; the dosage of carbendazim is preferably 0.8-1.8 g / L; the spraying amount of the fungicide is preferably 100-300 mL / m 2 ; More preferably 150 to 250 mL / m 2 . Spraying the nutrient solution A and fungicide can improve the survival rate and growth rate of Limonium fulva and avoid the influence of diseases and pests. The nutrient solution B is composed of the following components in mass fraction: 0.2-1% sodium nitrophenolate, 0.01-0.03% triacontanol, 0.1-0.3% alkylphenol polyoxyethylene ether and the balance water; the dosage of the sodium nitrophenolate is preferably 0.4-0.8%; the dosage of the triacontanol is preferably 0.015-0.025%; the dosage of the alkylphenol polyoxyethylene ether is preferably 0.15-0.25%; alkylphenol polyoxyethylene ether has good wetting, penetration, emulsification, dispersion and solubilization effects; the spraying amount of the nutrient solution B is preferably 100-200mL / m 2 , more preferably 120 to 180 mL / m 2. When the nutrient solution B is sprayed, the sodium nitrophenolate and triacontanol can increase the retention and deposition of the nutrient solution on the leaves under the action of alkylphenol polyoxyethylene ether, thereby improving the plant's utilization efficiency of the nutrient solution. After contacting the plant, it can quickly penetrate into the plant body and be quickly absorbed by the stems and leaves of the plant, accelerating the growth rate of the plant, promoting rooting, stem and leaf growth and development, thereby greatly improving the survival rate of Limonium eryngii and shortening the growth period of Limonium eryngii. The temperature of the greenhouse culture is preferably 15-25°C, more preferably 18-22°C; the lighting time of the greenhouse culture is preferably 17-19h / d, more preferably 17.5-18.5h / d.

[0025] In the present invention, Limonium tiliaceum plants with excellent performance are selected and threshed to obtain Limonium tiliaceum seeds, and the Limonium tiliaceum seeds are continuously selected and cultivated for 3 to 5 times according to steps 1) to 2) to obtain domesticated Limonium tiliaceum seeds.

[0026] The technical solutions provided by the present invention are described in detail below in conjunction with the embodiments, but they should not be construed as limiting the protection scope of the present invention.

[0027] Example 1

[0028] The wild seeds of Limonium sibiricum were first soaked in 3.5 mg / L gibberellin and 150 mg / L indoleacetic acid for 25 hours, then soaked in 0.2% potassium permanganate solution for 2 hours and then dried for later use. 2 The density is sown on a substrate with a moisture content of 12%. The substrate consists of the following components in parts by weight: 20 parts of peat soil, 20 parts of perlite, 10 parts of borax, 10 parts of sandy loam and 5 parts of sterilized humus soil. The sowing depth is 0.8 cm. Seedlings are cultivated. After the seedlings grow to 25 cm, nutrient solution A and fungicide are sprayed respectively. The formula of nutrient solution A is: 3.5 g / L amino acids, 1.5 g / L zinc sulfate, 2 g / L brassinolide, 10 mg / L amine hexadecene, 1 g / L rhamnolipid, 40 mg / L gibberellin, and the rest is water; the spraying amount of nutrient solution A is 300 mL / m 2 The formula of fungicide is: 1.5g / L of root rot, 1.5g / L of carbendazim, and the rest of water. The spraying amount of fungicide is 200mL / m 2 ; After that, nutrient solution B was sprayed every 10 days. The formula of nutrient solution B was: 0.6% sodium nitrophenolate, 0.02% triacontanol, 0.2% alkylphenol polyoxyethylene ether and the balance water; the spraying rate of nutrient solution B was 150mL / m 2; Cultivate the Limonium sibiricum plants in a greenhouse at a temperature of 20°C and a light duration of 18h / d. Select the Limonium sibiricum plants with excellent performance for threshing to obtain Limonium sibiricum seeds, and carry out breeding and cultivation of the Limonium sibiricum seeds for four consecutive times according to the above steps to obtain domesticated Limonium sibiricum seeds.

[0029] Example 2

[0030] The wild seeds of Limonium sibiricum were first soaked in 2 mg / L gibberellin and 100 mg / L indoleacetic acid for 18 hours, then soaked in 0.1% potassium permanganate solution for 1 hour and dried for later use. 2 The density is sown on a substrate with a moisture content of 10%. The substrate consists of the following components in parts by weight: 15 parts of peat soil, 15 parts of perlite, 5 parts of borax, 5 parts of sandy loam and 2 parts of sterilized humus soil. The sowing depth is 0.5 cm. Seedlings are cultivated. After the seedlings grow to 15 cm, nutrient solution A and fungicide are sprayed respectively. The formula of nutrient solution A is: 2g / L amino acid, 0.5g / L zinc sulfate, 1g / L brassinolide, 5mg / L amine hexadecene, 0.5g / L rhamnolipid, 25mg / L gibberellin, and the rest is water; the spraying amount of nutrient solution A is 200mL / m 2 The formula of fungicide is: 0.5g / L of root rot, 0.5g / L of carbendazim, and the rest of water. The spraying amount of fungicide is 100mL / m 2 ; After that, nutrient solution B was sprayed every 7 days. The formula of nutrient solution B was: 0.2% sodium nitrophenolate, 0.01% triacontanol, 0.1% alkylphenol polyoxyethylene ether and the balance water; the spraying rate of nutrient solution B was 100mL / m 2 ; Cultivate the Limonium sibiricum plants in a greenhouse at a temperature of 15°C and a light duration of 17h / d. Select the Limonium sibiricum plants with excellent performance for threshing to obtain Limonium sibiricum seeds, and carry out breeding and cultivation of the Limonium sibiricum seeds for three consecutive times according to the above steps to obtain domesticated Limonium sibiricum seeds.

[0031] Example 3

[0032] The wild seeds of Limonium chrysantha were first soaked in 5 mg / L gibberellin and 200 mg / L indoleacetic acid for 30 hours, then soaked in 0.3% potassium permanganate solution for 3 hours and then dried for standby use. The dried wild seeds of Limonium chrysantha were sown at a density of 5 seeds / cm2 on a substrate with a moisture content of 15%, the substrate consisting of the following components by weight: 25 parts of peat soil, 25 parts of perlite, 15 parts of borax, 15 parts of sandy loam and 8 parts of sterilized humus soil, and the sowing depth was 1 cm. Seedlings were cultivated. When the seedlings grow to 35cm, spray nutrient solution A and fungicide respectively. The formula of nutrient solution A is: amino acid 5g / L, zinc sulfate 2g / L, brassinolide 3g / L, diethylaminoethyl 15mg / L, rhamnolipid 2g / L, gibberellin 50mg / L, and the rest is water; the spraying amount of nutrient solution is 400mL / m2, the formula of fungicide is: root rot 2g / L, carbendazim 2g / L, the rest is water, and the spraying amount of fungicide is 300mL / m 2 ; Afterwards, spray nutrient solution B once every 15 days, the formula of nutrient solution B is: 1% sodium nitrophenolate, 0.03% triacontanol, 0.3% alkylphenol polyoxyethylene ether and the balance of water; the spraying amount of nutrient solution B is 200mL / m2; cultivate in a greenhouse at a temperature of 25°C and a light duration of 19h / d to obtain yellow flower Limonium plants. Select yellow flower Limonium plants with excellent performance for threshing to obtain yellow flower Limonium seeds, and carry out breeding and cultivation of the yellow flower Limonium seeds for 5 consecutive times according to the above steps to obtain domesticated yellow flower Limonium seeds.

[0033] Experimental Example 1

[0034] 1. Effects of different seed soaking methods on the germination of Limonium scabra seeds:

[0035] The specific process is as follows:

[0036] Nine groups A, B, C, D, E, F, G, H, and I were set up for control experiments. A was soaked according to the method of Example 1, B was soaked according to the method of Example 2, C was soaked according to the method of Example 3, D was soaked with only 3.5 mg / L gibberellin and 150 mg / L indoleacetic acid, E was soaked with only 0.2% potassium permanganate solution, F was soaked with 3.5 mg / L gibberellin and 0.2% potassium permanganate, G was soaked with 150 mg / L indoleacetic acid and 0.2% potassium permanganate, and the treatment methods of H and I were the same as those of Example 1, except that the concentration of indoleacetic acid was different.

[0037] Experimental results: as shown in Table 1.

[0038] Table 1 Germination rate of Limonium truncatum seeds under different seed soaking treatments

[0039]

[0040]

[0041] As can be seen from Table 1, the germination rates of the seed soaking treatment combinations of Examples 1 to 3 are all higher than those of the other treatments. It can be seen from A, H and I that with the increase in the concentration of indoleacetic acid, the germination rate first increases and then decreases, indicating that the use of an appropriate concentration of indoleacetic acid can increase the germination rate of Limonium truncatum seeds. It can be seen from A, D, E, F and G that the germination rate of Limonium truncatum seeds will be greatly reduced if any one of the components of gibberellins, indoleacetic acid and potassium permanganate solution is lacking, indicating that the synergistic cooperation between gibberellins, indoleacetic acid and potassium permanganate significantly improves the germination rate of Limonium truncatum seeds.

[0042] Experimental Example 2

[0043] Effects of different light durations and nutrient solution A on the growth period of Limonium fulva

[0044] The specific process is as follows: 13 groups of A, B, C, D, E, F, G, H, I, J, K, L, and M are set to conduct a control experiment. Among them, A is the same as Example 1, B is the same as Example 2, C is the same as Example 3, D changes the illumination time of Example 1 to 16h / d, E changes the illumination time of Example 1 to 20h / d, F changes the concentration of brassinolide in the nutrient solution A of Example 1 to 0.5g / L, G changes the concentration of brassinolide in the nutrient solution A of Example 1 to 5g / L, H changes the concentration of aminoethyl hexanoate in the nutrient solution A of Example 1 to 3mg / L, I changes the concentration of aminoethyl hexanoate in the nutrient solution A of Example 1 to 20mg / L, J changes the concentration of rhamnolipid in the nutrient solution A of Example 1 to 0.3g / L, K changes the concentration of rhamnolipid in the nutrient solution A of Example 1 to 3g / L, L changes the gibberellin in the nutrient solution A of Example 1 to 20mg / L, and M changes the gibberellin in the nutrient solution A of Example 1 to 60mg / L. Count the number of days in the entire growing period of Limonium xanthophyllum.

[0045] Experimental results: as shown in Table 2.

[0046] Table 2 Effects of different light duration and nutrient solution A on the whole growth period of Limonium fulva

[0047]

[0048]

[0049]

[0050] As can be seen from Table 2, under normal conditions, the entire growth period of Limonium chrysantha is more than one year, and the treatment methods of Examples 1 to 3 can significantly shorten the growth period of Limonium chrysantha. As can be seen from A, D and E, too long or too short a light exposure time is not conducive to shortening the growth period of Limonium chrysantha. As can be seen from A and F to M, the growth period of Limonium chrysantha can only be significantly shortened if the concentrations of the components in nutrient solution A cooperate with each other within a reasonable range. Too high or too low a concentration is not conducive to the rapid growth and development of Limonium chrysantha.

[0051] Experimental Example 3

[0052] Determination of production performance of Limonium truncatum seedlings:

[0053] Experimental location: This experiment was conducted in the native grass germplasm resource garden in April 2022. The native grass germplasm resource garden is located in Yueliangwan Park, Guide County. It has a plateau continental climate with long sunshine time and strong solar radiation. The spring is dry and windy, the summer is short and cool, the autumn is damp and rainy, the winter is long and dry, and the daily temperature difference is large. The annual average temperature is 9.5℃, the extreme maximum temperature is 34.5℃, and the extreme low temperature is -13.6℃. The annual precipitation is 184 mm, the frost-free period is 220 days throughout the year, the crop growing period is 249 days, and the annual sunshine hours are 2798 hours.

[0054] Test varieties:

[0055] The test species were the seeds of Limonium candidum obtained after cultivation and domestication in Example 1, and the control species were the local original wild Limonium candidum.

[0056] Planting method: According to the conventional planting technology of Limonium truncatum, investigate and record the phenological period, growth and development indicators, grain shed, seed yield and stress resistance of Limonium truncatum.

[0057] 1. Phenological periods include sowing period, initial flowering period, full flowering period, yellowing period, inflorescence withering period, and inflorescence viewing period. Bud period: 50% of the plants sprout after wintering. Initial flowering period: the date when 10% of the plant inflorescences unfold. Full flowering period: the date when 60% of the plant inflorescences unfold. Inflorescence withering period: the date when 50% of the plant inflorescences wither. Yellowing period: the date when 50% of the plants wither. Inflorescence viewing period: the number of days from the initial flowering period to the inflorescence withering period.

[0058] Experimental results: as shown in Table 3.

[0059] Table 3 Results of phenological period determination of different Limonium truncatum seeds

[0060]

[0061] It can be seen from Table 3 that compared with wild yellow-flowered Limonium, domesticated yellow-flowered Limonium has an earlier initial flowering period, an earlier peak flowering period, and a longer flowering period, which correspondingly extends its reproductive growth period.

[0062] 2. Determination of growth and development indicators, seed shattering and seed yield

[0063] Growth and development indicators include plant height, crown width, and root length. Plant height: Randomly select 10 plants from each plot during the flowering period, measure the absolute height from the ground to the highest part of the plant (excluding the awn), and calculate the average value in cm. Crown width: Measure the crown width of the plant using the plant height measurement sample during the flowering period, and calculate it in cm. Root length: Measure the distance from the base of the plant stem to the end of the root, and calculate the average value in cm.

[0064] Experimental results: as shown in Table 4.

[0065] Table 4 Results of the growth and development indexes, seed shattering and seed yield determination of different Limonium truncatum seeds

[0066] variety Plant heightcm Crown width cm Root length cm Particle shedding % <![CDATA[Seed yield kg / hm 2 > Domesticated species 49.7 47.9 58.3 21.3 18.0 Wild species 32.4 34.1 24.6 63.1 10.5

[0067] As shown in Table 4, there are significant differences in plant height, crown width and root length between domesticated Limonium chinense and wild Limonium chinense, and domesticated Limonium chinense is superior to wild Limonium chinense. From the perspective of seed maturation, the seeds of wild Limonium chinense mature inconsistently and have strong seed shedding, with a seed shedding rate as high as 63.1%; while domesticated Limonium chinense is easier to harvest than the original wild population, and has less seed shedding. By promoting the rapid growth of Limonium chinense, extending its reproductive growth period and reducing its seed shedding, the seed yield can reach 18.0 kg / hm 2 .

[0068] 3. Determination of stress resistance

[0069] The stress resistance is evaluated in terms of cold resistance, drought resistance, saline-alkali resistance, disease resistance, and insect resistance.

[0070] Experimental results: as shown in Table 5.

[0071] Table 5 Results of stress resistance test of different Limonium truncatum seeds

[0072] variety cold resistance Drought tolerance Salt and alkali resistance Disease resistance Insect resistance Domesticated species +++ +++ +++ +++ +++ Wild species ++ ++ ++ + +

[0073] Note: The plus sign indicates the grade or degree of stress resistance of Limonium xanthophyllum seeds, +++ indicates high stress resistance; ++ indicates medium stress resistance; + indicates low stress resistance.

[0074] As shown in Table 5, the domesticated Limonium truncatum is superior to the wild Limonium truncatum in cold resistance, drought resistance, heat resistance, disease resistance and insect resistance, showing strong adaptability.

[0075] It can be seen from the above embodiments and experimental examples that the yellow flower Limonium domesticated by the method of the present invention has excellent properties such as short growth period, less seed shedding, uniform maturity, high survival rate, high yield and strong stress resistance compared with wild yellow flower Limonium.

[0076] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A method for domesticating wild seeds of Limonium truncatum, characterized in that: The following steps are involved: 1) Soak the wild seeds of Limonium truncatum in growth regulator and potassium permanganate solution in turn and then dry them; The growth regulator is 2-5 mg / L of gibberellin and 100-200 mg / L of indoleacetic acid; the soaking time of the growth regulator is 18-30 hours; The concentration of the potassium permanganate solution is 0.1-0.3%, and the soaking time of the potassium permanganate solution is 1-3 hours; 2) Sowing the dried wild seeds of Limonium sibiricum to obtain seedlings, spraying the seedlings with nutrient solution A and fungicide after they grow to 15 to 35 cm, and then spraying them with nutrient solution B every 7 to 15 days to obtain Limonium sibiricum plants in a greenhouse; The temperature of the greenhouse culture is 15-25°C, and the light exposure time of the greenhouse culture is 17-19h / d; The nutrient solution A uses water as solvent and includes the following components in concentrations: 2-5 g / L amino acids, 0.5-2 g / L zinc sulfate, 1-3 g / L brassinolide, 5-15 mg / L diethylaminoethyl, 0.5-2 g / L rhamnolipid, and 25-50 mg / L gibberellin. The spraying amount of the nutrient solution A is 200-400 mL / m 2 ; The nutrient solution B is composed of the following components by mass fraction: 0.2-1% sodium nitrophenolate, 0.01-0.03% triacontanol, 0.1-0.3% alkylphenol polyoxyethylene ether and the balance water; the spraying amount of the nutrient solution B is 100-200 mL / m 2 ; 3) Selecting Limonium candidum plants with excellent performance and threshing them to obtain Limonium candidum seeds, and continuously breeding and cultivating the Limonium candidum seeds for 3 to 5 times according to steps 1) to 2) to obtain domesticated Limonium candidum seeds.

2. The acclimation method according to claim 1, characterized in that: The sowing matrix in step 2) is composed of the following components in parts by weight: 15-25 parts of peat soil, 15-25 parts of perlite, 5-15 parts of borax, 5-15 parts of sandy loam and 2-8 parts of sterilized humus soil; the water content of the sowing matrix is ​​10-15%.

3. The acclimation method according to claim 1, characterized in that: The sowing density in step 2) is 2 to 5 seeds / cm 2 ; The sowing depth is 0.5 to 1 cm.

4. The acclimation method according to claim 1, characterized in that: The fungicide in step 2) uses water as solvent and includes the following components in concentrations: 0.5-2 g / L of root rot and 0.5-2 g / L of carbendazim; the spraying amount of the fungicide is 100-300 mL / m 2 .

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