Cosmetic composition for enhancing mitochondrial energy, use and cosmetics

Abstract

The present invention belongs to the field of daily chemical new materials, and discloses a cosmetic composition for enhancing mitochondrial energy, comprising sodium mannose phosphate, carnosine, nicotinamide and plant extracts; the plant extracts are one or more combinations of liposomes of Milostachys sylvatica leaf / stem extract, Selaginella pulvinata extract and Balkania balkanica leaf extract. The composition can enhance the mitochondrial energy in cells, thereby promoting the synthesis of collagen, enhancing the skin barrier, regulating oil secretion, enhancing skin glossiness and resisting skin fatigue. At the same time, the present invention also provides the use and cosmetics of the composition.

Description

Technical Field

[0001] The invention relates to the field of new daily chemical materials, in particular to a cosmetic composition for enhancing mitochondrial energy, use and cosmetics. Background Art

[0002] Mitochondria are important organelles that play a vital role in multiple cellular mechanisms, including energy production and free radical maintenance. Mitochondrial dysfunction is characterized by decreased respiratory capacity per mitochondria and reduced mitochondrial membrane potential, often accompanied by elevated ROS production, and is considered a cause and hallmark of cellular aging.

[0003] Mitochondrial dysfunction can reduce ECM synthesis in fibroblasts, reduce skin elasticity, and thus cause wrinkles; disrupt melanin metabolism in melanocytes, causing dull and dark skin; unbalanced oil synthesis in sebaceous gland cells; impaired differentiation and keratinization of epidermal cells, defective barrier function, and increased cell death; and disordered lymphocyte signaling and function, causing inflammation.

[0004] Precision skin care acts on the mitochondria, the cell's energy station, helping to restore mitochondrial ATP energy and improve mitochondrial function.

[0005] The relationship between some components and mitochondrial energy enhancement and repair is disclosed in the following literature:

[0006] CN112891246A discloses a composition capable of repairing skin damaged by staying up late, wherein the composition is compounded by carnosine, bifid yeast fermentation product lysate, amber and panthenol, and the weight proportions of each raw material in the composition are as follows: 3-5 parts of carnosine, 3-5 parts of bifid yeast fermentation product lysate, 1-2 parts of amber, and 5-8 parts of panthenol. Its specification states: The composition is compounded by carnosine, bifid yeast fermentation product lysate, amber and panthenol, which can protect mitochondria, regulate the mitochondrial biological clock from the source, unblock mitochondrial vitality, activate cell energy supply, and enhance cell activity and skin function. The skin can play a self-repairing function at night, and even if the daily routine is disrupted, it can still maintain the healthy circulation of the skin and prevent skin aging.

[0007] CN116687781A discloses a composition for enhancing mitochondrial energy and its application, wherein the composition for enhancing mitochondrial energy comprises adenosine, sodium mannose phosphate and mannose. Its specification also records that its skin conditioner comprises niacinamide. The components in the composition for enhancing mitochondrial energy are reasonably matched and have a synergistic effect, which can not only provide substances and energy for biochemical processes, but also improve the skin's own antioxidant capacity, maintain cell homeostasis, promote collagen synthesis, and significantly improve skin aging.

[0008] CN116747158A discloses a cosmetic composition for anti-wrinkle and firming through mitochondria and its application. The cosmetic composition comprises sodium mannose phosphate, Gigartinastellata extract and Undaria pinnatifida extract, wherein, by weight ratio, sodium mannose phosphate: Gigartinastellata extract: Undaria pinnatifida extract = (1-50): (1-50): (1-50). The research results show that the cosmetic composition can remove free radicals for aging skin, promote metabolism, promote collagen synthesis, accelerate skin renewal, improve skin toughness from the cellular energy level, make the skin strong and smooth, elastic, firm and plump, and fade fine lines.

[0009] From the above records, it can be seen that carnosine, adenosine, sodium mannose phosphate, etc. all have the function of enhancing or repairing mitochondrial energy. At the same time, some plant extracts can be combined with sodium mannose phosphate to improve mitochondrial energy enhancement or repair.

[0010] The technical problem to be solved by this solution is: how to improve the mitochondrial energy enhancement and repair capabilities of cosmetics. Summary of the invention

[0011] The purpose of the present invention is to provide a cosmetic composition for enhancing mitochondrial energy, which can enhance the mitochondrial energy in cells, thereby promoting the synthesis of collagen, enhancing the skin barrier, regulating oil secretion, enhancing skin gloss, and resisting skin fatigue.

[0012] Meanwhile, the invention also provides the use and cosmetics of the composition.

[0013] To achieve the above object, the present invention provides the following technical solutions:

[0014] A cosmetic composition for enhancing mitochondrial energy, comprising sodium mannose phosphate, carnosine, niacinamide and a plant extract complex;

[0015] The plant extract complex is one or more combinations of a Milostachys chinensis leaf / stem extract complex, a Selaginella pulvinata extract complex, and a Balkania serrata leaf extract liposome complex.

[0016] Sodium mannose phosphate is a metabolite of the human body. It can directly participate in many metabolic pathways in the body, including the synthesis of amino acids, the synthesis of fatty acids, and gluconeogenesis. It produces and provides ATP energy to cells during metabolism. Sodium mannose phosphate is converted into fructose-6-phosphate by phosphomannose isomerase and enters the glycolysis pathway. Through the glycolysis pathway, 1 molecule of mannose-6-phosphate can generate 3 molecules of ATP and 2 molecules of NADH. Compared with the normal glycolysis pathway starting from glucose, sodium mannose phosphate can consume 1 less ATP molecule because it has a phosphate bond, which plays an energy-saving role.

[0017] Nicotinamide (NAM) is the amide form of nicotinic acid and one of the precursors of NAD+ (nicotinamide adenine dinucleotide). NAD+ is a coenzyme widely present in organisms and participates in many biochemical reactions. Together with ATP (adenosine), it serves as a key substance for energy metabolism. In addition, NAD+ is also involved in the intracellular respiration process, in which NAD+ reacts with oxygen to generate more stable NADH, an important energy carrier. This process will produce some free radicals, but NAD+ can effectively remove these free radicals through its own structural characteristics, thereby avoiding oxidative stress.

[0018] At the same time, niacinamide itself also exhibits a certain free radical scavenging ability when acting in non-cellular matrices.

[0019] Carnosine is a dipeptide composed of β-alanine and L-histidine, which exists in many tissues of the body, especially in muscle and brain tissue. It has a good effect of inhibiting lipid oxidation caused by free radicals and metal ions, so it can protect mitochondrial function and delay aging by inhibiting the production of free radicals.

[0020] Milo is native to southern Africa and is one of the few extremely drought-resistant plants on Earth. This plant contains a very efficient mixture of multiple active ingredients that provide resurrection and regeneration capabilities. Due to its unique drought resistance, it can achieve a strong ability to replenish and lock water, and it also has a highly efficient antioxidant capacity.

[0021] Balkan lettuce is a Mediterranean climate plant with a miraculous resurrection ability and extremely tenacious vitality: when summer comes and the climate is dry and rainless, it will go into "hibernation"; once winter comes, Balkan lettuce will quickly "revive" from months of dryness and regain vitality without any damage. Balkan lettuce extract has the antioxidant effect of removing ROS and can also reduce the content of oxidized proteins. By using phospholipids, cholesterol and fatty acid compounds to encapsulate Balkan lettuce extract to form liposomes, the active ingredients can more easily penetrate into the deep layers of the skin to work.

[0022] Selaginella pulvinata is a drought-loving plant that grows in dry environments. It is native to the arid regions of Southeast Asia and East Asia and grows in Shandong and Hebei. It grows on the rocks on the edge of cliffs or mountains. Selaginella pulvinata is called the "resurrection plant" because it can survive in an almost completely dry environment and can immediately come back to life once it comes into contact with water. Selaginella pulvinata extract contains trehalose and stigma biflavonoids, which can resist free radical oxidation and increase ceramide, caspase 14 and filaggrin.

[0023] The synergistic mechanism between sodium mannose phosphate, carnosine, niacinamide and plant extracts is:

[0024] Mitochondrial energy is mainly improved through: 1. Directly improve mitochondrial ATP energy; 2. Increase mitochondrial membrane potential; 3. Inhibit free radicals in mitochondria and protect the integrity of mitochondrial function; 4. Increase the oxygen content in mitochondria and promote the aerobic respiration process of mitochondria; 5. Increase the NAD+ content in mitochondria. Sodium mannose phosphate has high-energy phosphate bonds, which can directly provide ATP energy to mitochondria and reduce energy consumption; carnosine can inhibit the production of free radicals, protect the integrity of mitochondrial function, and enable mitochondria to efficiently provide ATP energy; Nicotinamide is one of the precursors of NAD+, which can act as a shuttle bus to transfer electrons from one cell molecule to another. Together with its molecular counterpart NADH, it participates in various metabolic reactions through electron exchange, and this metabolic reaction generates ATP energy; the three plant extracts are rich in polyphenols, alkaloids, flavonoids and trehalose and other active ingredients, which can help mitochondria fight oxygen free radicals, protect mitochondrial function and morphology, increase the oxygen content in mitochondria, promote the aerobic respiration process of mitochondria, and also provide the material energy basis for the life activities of mitochondria.

[0025] The weight ratio of the sodium mannose phosphate, carnosine, nicotinamide and plant extract complex is 0.1-10:1-500:0.1-10:0.1-10.

[0026] In the above composition, the weight ratio of the sodium mannose phosphate, carnosine, niacinamide and plant extract complex is 0.7-6:1-250:0.6-1.2:0.1-6.

[0027] In the above composition, the plant extract complex is a combination of a Milostachys chinensis leaf / stem extract complex, a Selaginella pulvinata extract complex, and a Balkania serrata leaf extract liposome complex;

[0028] The weight ratio of the milo leaf / stem extract complex, the Selaginella pulvinata extract complex and the Balkan sedum leaf extract liposome complex is 0.1-10:0.1-10:0.1-10.

[0029] In the above composition, the weight ratio of the mirabilis leaf / stem extract complex, the Selaginella pulvinata extract complex, and the balkania leaf extract liposome complex is 0.1:1:1.

[0030] At the same time, the present invention also discloses the use of any of the above compositions in preparing cosmetics.

[0031] Finally, the present invention also discloses a cosmetic comprising 0.001-20 wt % of any one of the above compositions.

[0032] In the above-mentioned cosmetics, the dosage form of the cosmetics is essence, emulsion or cream.

[0033] Compared with the prior art, the present invention has the following beneficial effects:

[0034] The components of the composition for enhancing mitochondrial energy of the present invention are reasonably matched and have a synergistic effect. They can not only provide substances and ATP energy for the biochemical process of cells, maintain cell homeostasis, help skin cells maintain corresponding life activities, and enable fibroblasts in the skin to generate more type I collagen, thus having an anti-aging effect; they can also accelerate the differentiation of keratinocytes in the skin, speed up the metabolism of melanin in the stratum corneum of the skin, and reduce the formation of melanin, thereby having a whitening effect; they can also balance the secretion of oil by sebaceous gland cells in the skin, thereby reducing the production of oil and achieving the effect of oil control; in addition, the composition can also improve the skin's own antioxidant capacity and help remove free radicals in the skin. Therefore, in summary, the composition has the effects of whitening, anti-aging and oil control.

[0035] Its internal mechanism is:

[0036] Mitochondrial energy is mainly improved through: 1. Directly improve mitochondrial ATP energy; 2. Increase mitochondrial membrane potential; 3. Inhibit free radicals in mitochondria and protect the integrity of mitochondrial function; 4. Increase the oxygen content in mitochondria and promote the aerobic respiration process of mitochondria; 5. Increase the NAD+ content in mitochondria. Sodium mannose phosphate has high-energy phosphate bonds, which can directly provide ATP energy to mitochondria and reduce energy consumption; carnosine can inhibit the production of free radicals, protect the integrity of mitochondrial function, and enable mitochondria to efficiently provide ATP energy; Nicotinamide is one of the precursors of NAD+, which can act as a shuttle bus to transfer electrons from one cell molecule to another. Together with its molecular counterpart NADH, it participates in various metabolic reactions through electron exchange, and this metabolic reaction generates ATP energy; the three plant extracts are rich in polyphenols, alkaloids, flavonoids and trehalose and other active ingredients, which can help mitochondria fight oxygen free radicals, protect mitochondrial function and morphology, increase the oxygen content in mitochondria, promote the aerobic respiration process of mitochondria, and also provide the material energy basis for the life activities of mitochondria. DETAILED DESCRIPTION

[0037] The technical solution of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0038] Source of raw materials:

[0039] The sodium mannose phosphate was purchased from Beijing Yanzhishan Technology Co., Ltd.

[0040] The carnosine was purchased from Shenzhen Readline Biotechnology Co., Ltd.

[0041] The mithraea extract complex was purchased from RAHN AG;

[0042] The Balkan oleracea leaf extract liposome complex was purchased from Guangdong Heji Biotechnology Co., Ltd.

[0043] The Selaginella pulvinata extract complex is from Givaudan France SAS.

[0044] Among the raw materials used in the present invention, the Myrtle leaf / stem extract complex contains 1-5wt% of Myrtle leaf / stem extract, the Selaginella pulvinata extract complex contains 1-5wt% of Selaginella pulvinata extract, and the Balkan sedum leaf extract liposome complex contains 0.01-5wt% of Balkan sedum leaf extract. In actual applications, the net content of the active ingredients of the above plant extracts may change due to changes in suppliers.

[0045] The rest of the raw materials can be used as long as they are purchased from regular dealers.

[0046] The weight percentages of the carnosine, sodium mannose phosphate, niacinamide, Myrtlesia serrata extract complex, Balkan moss leaf extract complex and Selaginella pulvinata extract complex mentioned below are all calculated based on the actual content of effective ingredients contained in the commercially available raw materials. The commercially available raw materials may also selectively contain any one or a combination of at least two of solvents, fillers, diluents, stabilizers, pH adjusters, antibacterial agents, antioxidants or impurities within the permitted range.

[0047] Part I Examples and Comparative Examples

[0048] The formulation shown in Table 1 is used to prepare an aqueous solution, and the preparation method is as follows:

[0049] Step 1: Take an appropriate amount of deionized water, add sodium mannose phosphate, carnosine and niacinamide, stir and dissolve evenly, which is regarded as phase A;

[0050] Step 2: Add one or more of the complex of Milostachys chinensis leaf / stem extract, the complex of Selaginella pulvinata extract and the liposome complex of Balkania balkanica leaf extract to phase A, stir evenly, and add water to 100g; the formula can be seen in Table 1;

[0051] Table 1 Recipe

[0052]

[0053]

[0054] DPPH Antioxidant Test

[0055] Take 1g of the solution of Example 1-12 and Comparative Example 1-11, add 2mL of anhydrous ethanol, add 2mL of 60μmol / L DPPH solution after complete dissolution, mix, let stand for 30min, adjust to zero with anhydrous ethanol, and measure the absorbance at 517nm as Ai; take 2mL of anhydrous ethanol solvent + 2mL DPPH solution in the same way and mix to determine the absorbance extreme Ac; take 1g of the matrix of the composition and dissolve it, add 2mL of anhydrous ethanol, then add 2mL of anhydrous ethanol solvent and mix to determine the absorbance as Aj.

[0056] The free radical scavenging rate was calculated according to the following formula.

[0057] Clearance (%) = [1-(Ai-Aj) / Ac] × 100%

[0058] Among them, Aj is the contribution of the reaction sample itself to the absorbance; Ai is the absorbance value of the sample after the action of DPPH; Ac is the absorption of DPPH itself at the measurement wavelength.

[0059] The DPPH free radical scavenging rate reflects its antioxidant capacity to a certain extent. The higher the scavenging rate, the better the antioxidant degree and the better the anti-aging effect. Therefore, the anti-aging effect of the composition can be judged by studying its ability to scavenge DPPH free radicals. The results are shown in Table 2 (each group of samples was measured 5 times in parallel, and the data are presented as average values).

[0060] The specific test results are shown in Table 2:

[0061] Table 2 DPPH clearance test results

[0062]

[0063]

[0064] The following conclusions can be drawn from the experimental results in Table 2:

[0065] 1. Comparative Examples 5 and 6 show that the combination of sodium mannose phosphate, carnosine and nicotinamide has limited effect in the removal of DPPH;

[0066] It can be seen from Comparative Examples 1, 2, 3 and 4 that the DPPH scavenging effect of the Myrtlesia leaf / stem extract complex, the Selaginella pulvinata extract complex and the Balkan Herbaceous ...

[0067] The effects of each embodiment are significantly improved, and the possible reason is that carnosine has the ability to inhibit the generation of free radicals, and nicotinamide has a certain free radical scavenging ability. After being compounded with the milanoderma leaf / stem extract complex, the selaginella pulvinata extract complex and the balkan sedum leaf extract liposome complex, the free radical scavenging ability of the composition can be improved;

[0068] 2. By comparing Examples 1 to 3 and Comparative Examples 2 to 5, it can be seen that when the two are combined, they have obvious synergy; it can be seen from Examples 2, 6 and 7 that the Selaginella pulvinata extract complex only needs to be controlled at 0.0001g in dosage to show very good effects, and increasing the dosage will not show further synergy; it can be seen from Examples 3, 8 and 9 that when the dosage of the Milo leaf / stem extract complex reaches 0.001g, the most efficient synergy is achieved, and increasing the dosage will not show better synergy; it can be seen from Examples 1, 4 and 5 that the three have similar performances, that is, within the reasonable dosage range of the present invention, the consistency of performance will be shown;

[0069] 3. It can be seen from Examples 10 and 11 that when two or three plant extracts are used, the performance is dramatically improved, especially when three plant extracts are present at the same time, the free radical scavenging effect is significantly improved.

[0070] The above results can further speculate the possibility of antioxidant application of the above composition at the cellular level: under the premise of ensuring sufficient energy for mitochondria, the three plant extracts are rich in polyphenols, alkaloids, flavonoids and trehalose and other active ingredients, which can help mitochondria fight oxygen free radicals, protect mitochondrial function and morphology, increase the oxygen content in mitochondria, promote mitochondrial aerobic respiration, and also provide material energy basis for mitochondrial life activities, thus significantly improving the antioxidant capacity of mitochondria. In order to verify this speculation, the following collagen test, ATP content test, and zebrafish freckle removal and whitening test were conducted. Due to the cost limitation of the experiment, only some cases were verified.

[0071] Part II Cell and zebrafish testing

[0072] Collagen test

[0073] (1) Preparation of dermal fibroblast photoaging model

[0074] Skin fibroblast HSF cells that are in good growth state and in the middle and late logarithmic growth stage were taken, the cells were digested and counted, the cell density was adjusted, and the cells were inoculated into a 24-well cell culture plate (sterile cell slides were pre-added in the culture plate), and placed in a cell culture incubator for 24 hours;

[0075] Set up a normal control group, a model group, and a sample group, discard the culture medium in the wells, divide the groups into experimental groups, add the corresponding sample working solution to each group, and place them in a cell culture incubator for 24 hours;

[0076] The cell plate was removed, the supernatant was discarded, and the cells were washed with PBS (phosphate buffered saline). The cells in the model group and the sample group were exposed to UVA for irradiation. After the irradiation, the PBS was discarded, and the corresponding working solution was added to each group, and the cells were placed in a cell culture incubator for further culture for 48 hours.

[0077] (2) Immunofluorescence staining of collagen

[0078] Take out the culture plate, discard the culture medium in the wells, add PBS to wash and then discard;

[0079] Add paraformaldehyde fixative to fix the cells, add PBS to wash and discard;

[0080] Add Triton X-100 solution for permeabilization, add PBS for washing and discard;

[0081] Add bovine serum albumin solution for blocking, add PBS for washing and discard;

[0082] Add Rabbit Anti-Collagen I antibody and Goat Anti-Rabbit IgG H&L (Alexa 488) for staining and incubation, and then PBS was added for washing and discarded;

[0083] Add a drop of anti-fluorescence quencher on the slide, cover the cell slide, observe and take pictures under a fluorescence microscope.

[0084] (3) Calculation of relative fluorescence intensity results

[0085] ImageJ 1.53t software was used to measure the fluorescence intensity and calculate the corrected total cell fluorescence (CTCF). The calculation formula is as follows:

[0086] CTCF = cumulative optical density - area of ​​the cell region to be tested × background average optical density relative fluorescence intensity (%) = CTCF e / CTCF b ×100%

[0087] Where: CTCF e —CTCF of sample group and model group; CTCF b —CTCF of normal control group.

[0088] Data were analyzed using GraphPad Prism 7.00 software and independent sample t-test, and p < 0.05 was considered statistically significant.

[0089] The test results can be seen in Table 3;

[0090] Table 3 Type I collagen content

[0091]

[0092] As can be seen from Table 3, the content of type I collagen in Example 11 and Example 12 is higher than that in Comparative Example 1, Comparative Example 5 and Comparative Example 6, indicating that sodium mannose phosphate, carnosine, niacinamide, Balkan moss leaf extract, Milo leaf / stem extract and Selaginella pulvinata extract have a good synergistic effect with each other.

[0093] ATP content test

[0094] 2×10 5 HaCaT cells were uniformly inoculated into 6-well plates at a concentration of 1 cell / well, cultured at 37°C, 5% CO2 for 24 hours, and the supernatant in the wells was aspirated before drug administration and continued to be cultured for 24 hours. A normal control group and a sample group were set up in the experiment; the culture medium was aspirated, and 200 μL of lysis solution was added to each well for lysis on ice. After standing for 10 minutes, the cells were repeatedly blown and mixed, and centrifuged at 4°C 12000g for 5 minutes. The supernatant was taken for subsequent determination, and the protein concentration in the supernatant was determined according to the method in the instructions of the BCA protein concentration determination kit.

[0095] Dilute the ATP standard solution with ATP detection lysate to an appropriate concentration gradient: 0.01, 0.03, 0.1, 0.3, 1, 3 and 10 μM (in subsequent experiments, the concentration range of the standard can be adjusted appropriately according to the concentration of ATP in the sample). Take an appropriate amount of ATP detection reagent and dilute the ATP detection reagent with ATP detection reagent diluent at a ratio of 1:9 to obtain the ATP detection working solution; add 100 μL of ATP detection working solution to a 96-well plate and place it at room temperature for 3-5 minutes to consume all the background ATP, thereby reducing the background; then add 20 μL of the sample or standard to be tested to the wells, mix quickly, and use a chemiluminometer to measure the RLU value or CPM after an interval of 3 seconds. Calculate the concentration of ATP in the sample to be tested based on the standard curve, and convert the concentration of ATP into the form of nmol / mg protein, that is, calculate the ATP concentration under unit concentration conditions.

[0096] The test results can be seen in Table 4;

[0097] Table 4 ATP content

[0098] Group ATP content (μmol / g prot) Improvement rate vs normal control group Normal control group 10.674 / Comparative Example 1 12.824 20.14％ Comparative Example 5 11.824 10.77％ Comparative Example 6 12.025 12.66％ Embodiment 11 14.310 34.07％ Example 12 14.552 36.33％

[0099] As can be seen from Table 4, the ATP content in Example 11 and Example 12 is higher than that in Comparative Example 1, Comparative Example 5 and Comparative Example 6, indicating that sodium mannose phosphate, carnosine, niacinamide, Balkan chayote leaf extract, Milo leaf / stem extract and Selaginella pulvinata extract have a good synergistic effect with each other.

[0100] Zebrafish freckle removal and whitening test

[0101] The zebrafish at 6 hours post-fertilization (6hpf) were divided into several groups, including a normal control group and a sample group. The normal control group was not treated, and the sample group was incubated in the dark for 45 hours with the test sample (the test sample was dissolved in the fish water, and the water-soluble drug was administered). The zebrafish head was photographed, and the melanin signal intensity of the zebrafish head was analyzed using advanced image processing software to calculate the relative melanin content of the zebrafish head in each group; the skin melanin signal intensity reflects the whitening effect of the test product.

[0102] The test results can be seen in Table 5;

[0103] Table 5 Melanin content in zebrafish head

[0104]

[0105] The lower the melanin intensity of the zebrafish head, the lower the relative melanin content, and the stronger the whitening effect. It can be seen from Table 5 that the melanin content of the zebrafish head in Example 11 and Example 12 is less than that in Comparative Example 1, Comparative Example 5 and Comparative Example 6, indicating that sodium mannose phosphate, carnosine, niacinamide, Balkan moss leaf extract, Myrtillus chinensis leaf / stem extract and Selaginella pulvinus extract have a good synergistic effect with each other.

[0106] Sebaceous gland cell oil control test

[0107] (1) Cell seeding: SZ95 cells were seeded at 1×10 5 Each well was inoculated into a 24-well plate and cultured in an incubator (37°C, 5% CO2) for 24 h. A normal control group (0 mM sample), a model control group (testosterone + 0 mM sample) and a sample group (testosterone + A mM sample) were set up respectively.

[0108] (2) Testosterone modeling + administration: After the cells adhered to the wall, according to the above grouping, the normal control group and the model control group were replaced with fresh culture medium, and the sample group was replaced with fresh culture medium containing the sample, and treated with testosterone to induce lipid synthesis. Then, they were placed in a 37°C, 5% CO2 incubator for another 24 hours;

[0109] (3) After the incubation, the culture medium was removed, the cells were washed three times with precooled D-Hank's solution, fixed with 60% isopropanol, washed with D-Hank's solution, stained with Nile red, and photographed with a fluorescence microscope. The fluorescence intensity of each group was analyzed with ImageJ. The normal control group was used as a reference, and the relative lipid content of each group was calculated based on the fluorescence intensity.

[0110] The test results can be seen in Table 6;

[0111] Table 6 Sebaceous gland cell oil content

[0112] Group Relative lipid content Inhibition rate vs model group Normal control group 1.000 / Model control group 15.024 / Comparative Example 1 1.463 90.26％ Comparative Example 5 14.685 2.25％ Comparative Example 6 14.253 5.13％ Embodiment 11 1.106 92.64％ Example 12 0.617 95.89％

[0113] The lower the relative lipid content of sebaceous gland cells, the stronger the oil control effect. It can be seen from Table 6 that the relative lipid content in Example 11 and Example 12 is less than that in Comparative Example 1, Comparative Example 5 and Comparative Example 6. However, from the perspective of synergy, no obvious synergy was seen. The specific reason is that the oil inhibition rate of the plant extract itself is already very good, and it is difficult to achieve substantial improvement even with the participation of other substances.

[0114] Part 3 Application Formulation

[0115] This application example prepares an anti-wrinkle essence lotion, and its formula is as shown in Table 7;

[0116] Table 7 Recipe

[0117]

[0118] Its preparation method is:

[0119] (1) Add deionized water, moisturizer and thickener to the water phase pot, stir and heat to 85°C and keep warm for 45 minutes; add emulsifier, emollient, antioxidant and preservative to the oil phase pot, stir and heat to 80°C and keep warm for 45 minutes;

[0120] (2) After the heat preservation is completed, the water and oil pots are pumped into the emulsification pot, homogenized at 65° C. and 800 rpm for 20 minutes, cooled to 35° C., and the composition for enhancing mitochondrial energy is added and mixed evenly to obtain the product.

[0121] This application example prepares an anti-wrinkle essence, and its formula is as shown in Table 8;

[0122] Table 8 Recipe

[0123]

[0124] Its preparation method is:

[0125] (1) Add deionized water, humectant and thickener into the water phase pot, stir and heat to 85°C and keep warm for 45 minutes, stirring evenly during the insulation process.

[0126] (2) After the heat preservation is completed, the temperature is lowered to 35° C., and the composition for enhancing mitochondrial energy and the preservative are added and mixed evenly to obtain the product.

[0127] It is obvious to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above, and that the present invention can be implemented in other specific forms without departing from the spirit or essential features of the present invention. Therefore, the embodiments should be regarded as exemplary and non-restrictive from any point of view, and the scope of the present invention is defined by the appended claims rather than the above description, and it is intended that all changes falling within the meaning and scope of the equivalent elements of the claims are included in the present invention.

Claims

1. A cosmetic composition for enhancing mitochondrial energy, characterized in that: Includes sodium mannose phosphate, carnosine, niacinamide, and a plant extract complex; The plant extract complex is a combination of a Milostachys chinensis leaf / stem extract complex, a Selaginella pulvinata extract complex, and a Balkania serrata leaf extract liposome complex; The weight ratio of the milo leaf / stem extract complex, the Selaginella pulvinata extract complex, and the Balkan sedum leaf extract liposome complex is 0.1-10:0.1-10:0.1-10; The weight ratio of the sodium mannose phosphate, carnosine, nicotinamide and plant extract complex is 0.1-10:1-500:0.1-10:0.1-10.

2. The composition according to claim 1, characterized in that The weight ratio of the sodium mannose phosphate, carnosine, nicotinamide and plant extract complex is 0.7-6:1-250:0.6-1.2:0.1-6.

3. The composition according to claim 1, characterized in that The weight ratio of the milo leaf / stem extract complex, the Selaginella pulvinata extract complex, and the Balkan sedum leaf extract liposome complex is 0.1:1:

1.

4. Use of the composition according to any one of claims 1 to 3 in preparing cosmetics.

5. A cosmetic, characterized in that: Contains 0.001 to 20 wt% of the composition according to any one of claims 1 to 3.

6. The cosmetic according to claim 5, characterized in that: The dosage form of the cosmetic is emulsion or cream.

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