A moisturizing composition and its preparation method and application

Abstract

The present invention belongs to the technical field of skin care products, and specifically relates to a moisturizing composition and its preparation method and application. The composition is composed of the following raw materials in percentage by mass: 5-10% of birch inonotus extract, 6-15% of purslane extract, 3-5% of aloe vera extract, 1-3% of sophora flavescens root extract, 7-9% of field mint leaf extract, 10-12% of golden chamomile extract, 15-20% of butanediol, 8-12% of glycerol, 0.5-1% of 1,2-hexanediol, 0.3-0.5% of parahydroxyacetophenone, and the balance is water. In the preparation process of birch inonotus extract, the present invention innovatively adds synephrine and sodium caseinate to the basic seed culture medium, and adds shikimic acid and sodium copper chlorophyll to the fermentation medium. The test results show that the addition of synephrine and shikimic acid effectively increases the polysaccharide content in birch inonotus extract, thereby improving the moisturizing effect of skin care products.

Description

Technical Field

[0001] The invention belongs to the technical field of skin care products, and specifically relates to a moisturizing composition and a preparation method and application thereof. Background Art

[0002] The body's moisture is mainly stored in the skin. Once the skin is dehydrated, it will cause various skin problems such as dryness, dullness, and roughness. With the improvement of living conditions and work needs, people are in closed air-conditioned rooms for a long time, which will cause the evaporation of skin moisture to accelerate, and with the increase of age, the skin moisture will also decrease physiologically. In addition, external environmental factors such as seasonal changes also have a great impact on skin moisture. Based on this, people have a great demand for skin hydrating and moisturizing products.

[0003] Inonotus obliquus Unknown oblique ) belongs to the phylum Basidiomycetes, class Hymenomycetes, order Aphyllophorales, family Pucciniaceae, and mainly parasitizes under the bark of standing trees of birch, silver birch, elm, alder and other trees or on the dead trunks of felled trees. It is mainly distributed in the northern hemisphere at 45-50 degrees north latitude. In my country, birch inonotus is mainly distributed in Heilongjiang Province and Changbai Mountain in Jilin Province. Studies have found that birch inonotus extract contains a variety of effective ingredients such as polysaccharides, triterpenes, steroids, polyphenols, etc. In addition, birch inonotus extract has significant antioxidant and anti-inflammatory effects, can improve skin texture and reduce fine lines, and has certain anti-aging and anti-wrinkle effects. Chinese patent CN117815096A discloses a moisturizing and repairing composition and its application, disclosing that birch inonotus extract has a moisturizing effect. Chinese patent CN117398326A discloses a skin moisturizing and repairing composition and its application, disclosing that birch inonotus extract can effectively strengthen the cohesion of stratum corneum cells and enhance the skin barrier function.

[0004] Although the prior art has disclosed a variety of preparation methods for Inonotus birch extracts, there is still room for improvement in the content of active ingredients and skin care effects. Chinese patent CN117398326A discloses a skin moisturizing and repairing composition and its application, and also discloses an Inonotus birch extract obtained by a subcritical water extraction process. Compared with the organic supercritical extraction process and the traditional water-alcohol extraction process, the obtained Inonotus birch extract has better moisturizing and repairing effects. Although the above extraction process can extract active ingredients with moisturizing effects, there are problems such as low extraction efficiency and insufficient content of active ingredients, especially in terms of polysaccharide content, which is difficult to meet skin care needs.

[0005] Based on this, it is necessary to improve the culture process and extraction process of Inonotus obliquus to significantly enhance the polysaccharide content therein, thereby accelerating its application in the field of skin care products. Summary of the invention

[0006] A first object of the present invention is to provide a moisturizing composition having an excellent moisturizing effect.

[0007] The second object of the present invention is to provide a method for preparing a moisturizing composition, which is simple and easy to operate.

[0008] The third object of the present invention is to provide an application of a moisturizing composition, which has broad application prospects.

[0009] In order to achieve the above object, the technical solution adopted by the present invention is:

[0010] A moisturizing composition, comprising the following raw materials in percentage by weight: 5-10% of Inonotus obliquus extract, 6-15% of Portulaca oleracea extract, 3-5% of Aloe vera extract, 1-3% of Sophora flavescens root extract, 7-9% of Mentha sabdariffa leaf extract, 10-12% of Matricaria chamomilla extract, 15-20% of butylene glycol, 8-12% of glycerol, 0.5-1% of 1,2-hexanediol, 0.3-0.5% of p-hydroxyacetophenone, and the balance of water;

[0011] The preparation process of the Inonotus obliquus extract is as follows:

[0012] (1) Inonotus obliquus strains are inoculated into a basic seed culture medium supplemented with sodium caseinate and synephrine for culturing to obtain mycelium of Inonotus obliquus;

[0013] (2) inoculating the mycelium of Inonotus obliquus obtained in step (1) into a fermentation medium supplemented with shikimic acid and sodium copper chlorophyll, and continuing to culture to obtain a product;

[0014] (3) The product obtained in step (2) is crushed, and then water is added for reflux extraction to obtain an extract, the extract is filtered and concentrated, and then ethanol is added for precipitation, the precipitate is collected, and the precipitate is dried to obtain an Inonotus obliquus extract.

[0015] Furthermore, the concentrations of sodium caseinate and synephrine in the basic seed culture medium in step (1) are: sodium caseinate 0.08-0.15 g / L, synephrine 1-5 mg / L; the nutritional composition of the basic seed culture medium is: glucose 15-20 g / L, peptone 1-5 g / L, potassium dihydrogen phosphate 0.5-1.5g / L, anhydrous magnesium sulfate 0.5-1.5g / L.

[0016] Furthermore, in step (1), the inoculation amount of the Inonotus obliquus strain is 8-10% of the total mass of the basic seed culture medium, the culture temperature is 20-30° C., and the culture time is 7-15 days.

[0017] Furthermore, the concentrations of shikimic acid and sodium copper chlorophyllin in the fermentation medium in step (2) are: 9-15 mg / L shikimic acid and 0.4-1 mg / L sodium copper chlorophyllin; the nutrient composition of the fermentation medium is: 15-20 g / L glucose, 2-6 g / L beef extract, and dipotassium hydrogen phosphate. 0.5-1.5g / L, anhydrous magnesium sulfate 0.5-1.5g / L, MnSO4·H2O 0.05-0.15g / L.

[0018] Furthermore, in step (2), the inoculation amount of the inonotus obliquus mycelium is 8-12% of the total mass of the fermentation medium; the temperature of the continued culture is 20-28° C., and the time is 10-14 days.

[0019] Furthermore, the reflux extraction time in step (3) is 2-3 hours.

[0020] The preparation method of the above-mentioned moisturizing composition comprises the following steps:

[0021] According to the said mass percentage, the raw materials are uniformly mixed to obtain the product.

[0022] The above-mentioned moisturizing composition is used in the preparation of skin care products.

[0023] Furthermore, based on the total mass of the skin care product, the mass percentage of the moisturizing composition is 1-5%.

[0024] Furthermore, the skin care product also includes the following raw materials in percentage by weight: carbomer 0.1-0.3%, PEG-40 hydrogenated castor oil 4-6%, behenyl alcohol 0.5-1%, and the balance is water.

[0025] Compared with the prior art, the beneficial effects of the present invention are mainly:

[0026] 1. In the preparation process of the Inonotus birchii extract, the present invention innovatively adds synephrine and sodium caseinate to the basic seed culture medium, and adds shikimic acid and sodium copper chlorophyllin to the fermentation medium. The polysaccharide content test results show that the addition of synephrine and shikimic acid can effectively increase the polysaccharide content in the Inonotus birchii extract, and the polysaccharide content in the Inonotus birchii extract of the present invention reaches more than 35.56wt%.

[0027] 2. The moisturizing composition provided by the present invention is mild and non-irritating to the skin, safe to use, and has excellent moisturizing effect. The results of the skin moisture content test show that after using the skin care product added with the moisturizing composition of the present invention for 28 days, the change rate of skin hydration content is more than 68.47%. DETAILED DESCRIPTION

[0028] The technical scheme of the present invention is further described below in conjunction with specific embodiments. However, it should be understood by those skilled in the art that the following examples are only used to illustrate the present invention and should not be regarded as limiting the present invention. The specific conditions not specified in the examples are carried out according to conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used, unless otherwise specified, are conventional products obtained through commercial channels.

[0029] Example

[0030] Example 1

[0031] A moisturizing composition is composed of the following raw materials in percentage by mass: 8% of Inonotus obliquus extract, 12% of Portulaca oleracea extract, 4% of Aloe vera extract, 2% of Sophora flavescens root extract, 8% of Mentha sabdariffa leaf extract, 11% of Matricaria chamomilla extract, 16% of butylene glycol, 9% of glycerol, 0.8% of 1,2-hexanediol, 0.4% of p-hydroxyacetophenone, and the balance of water.

[0032] The specific preparation process of the Inonotus obliquus extract is as follows:

[0033] (1) The inoculum strain was inoculated into a basic seed culture medium supplemented with sodium caseinate and synephrine at an inoculum amount of 9% of the total mass of the basic seed culture medium. The concentrations of sodium caseinate and synephrine in the basic seed culture medium were: sodium caseinate 0.1 g / L, synephrine 4 mg / L. The culture was carried out at 28°C for 12 days to obtain the mycelium of Inoculum birchii. The nutritional composition of the basic seed culture medium was: glucose 18 g / L, peptone 4 g / L, potassium dihydrogen phosphate 1g / L, anhydrous magnesium sulfate 1g / L.

[0034] (2) The inoculum of Inonotus obliquus obtained in step (1) is inoculated into a fermentation medium to which shikimic acid and sodium copper chlorophyllin are added, according to an inoculum amount of 10% of the total mass of the fermentation medium. The concentrations of shikimic acid and sodium copper chlorophyllin in the fermentation medium are: 12 mg / L shikimic acid and 0.5 mg / L sodium copper chlorophyllin. The culture is continued at 25° C. for 12 days to obtain a product. The nutrient composition of the fermentation medium is: 18 g / L glucose, 5 g / L beef extract, and dipotassium hydrogen phosphate. 1g / L, anhydrous magnesium sulfate 1g / L, MnSO4·H2O 0.1g / L.

[0035] (3) The product obtained in step (2) is crushed to a particle size of 5 mm, and water is added to reflux for extraction for 2.5 hours to obtain an extract. The extract is filtered and concentrated, and then ethanol is added to precipitate. The precipitate is collected and dried to obtain an Inonotus birchii extract.

[0036] The specific preparation process of purslane extract is as follows:

[0037] Fresh purslane was squeezed for 10 min at 45°C to obtain a squeezed juice and purslane residue; a 60% (v / v) ethanol aqueous solution in an amount of 8 times the mass of the purslane residue was added to the obtained purslane residue for alcohol extraction, and an extract A and a filter residue were obtained after solid-liquid separation; water in an amount of 8 times the mass of the filter residue was added to the filter residue for extraction, and an extract B was obtained after solid-liquid separation; the squeezed juice, the extract A and the extract B were combined, and then filtered using a 0.45 μm filter membrane to obtain a permeate, and the permeate was concentrated under reduced pressure to remove ethanol and water, and spray-dried to obtain a purslane extract.

[0038] The specific preparation process of Aloe vera extract is as follows:

[0039] Take mature aloe vera leaves with a length of ≥40 cm and a maximum width of ≥6 cm, wash them, remove the leaf epidermis, take the internal gelatinous mesophyll, stir and crush them, and squeeze out the juice. Centrifuge the squeezed juice at 5000 rpm for 60 minutes to obtain the supernatant, that is, the aloe vera extract.

[0040] The specific preparation process of Sophora flavescens root extract is as follows:

[0041] The root of Sophora flavescens was crushed into Sophora flavescens root powder, 5 times the amount of water was added to the Sophora flavescens root powder, extracted at 40°C for 15 hours, and filtered through a 200-mesh filter cloth for solid-liquid separation to obtain the first filter residue and filtrate. 8 times the amount of water was added to the first filter residue, refluxed for 5 hours, and filtered through a 200-mesh filter cloth to obtain the second filtrate. The first filtrate and the second filtrate were combined, concentrated, and dried to obtain the Sophora flavescens root extract.

[0042] The specific preparation process of field mint leaf extract is as follows:

[0043] Wash and crush the field mint leaves, add 2 times the amount of water to the field mint leaf powder, reflux extraction for 3 hours, cool, filter, concentrate and dry to obtain the field mint leaf extract.

[0044] The specific preparation process of golden chamomile extract is as follows:

[0045] Take golden chamomile and add it to 14 times the amount of ethanol aqueous solution, soak it for 1 hour, then heat and maintain slight boiling for extraction for 1 hour, filter the extract to obtain a filtrate, concentrate the filtrate to a relative density of 1.10, and spray dry it to obtain golden chamomile extract.

[0046] The preparation method of the above-mentioned moisturizing composition is as follows:

[0047] According to the above mass percentage, mix the raw materials evenly to obtain the product.

[0048] Example 2

[0049] A moisturizing composition is composed of the following raw materials in percentage by mass: 10% of Inonotus obliquus extract, 15% of Portulaca oleracea extract, 5% of Aloe vera extract, 3% of Sophora flavescens root extract, 9% of Mentha sabdariffa leaf extract, 12% of Matricaria chamomilla extract, 20% of butylene glycol, 12% of glycerol, 1,2-hexanediol, 0.5% of p-hydroxyacetophenone, and the balance of water.

[0050] The specific preparation process of the Inonotus obliquus extract is as follows:

[0051] (1) The inoculum strain was inoculated into a basic seed culture medium supplemented with sodium caseinate and synephrine at an inoculum amount of 10% of the total mass of the basic seed culture medium. The concentrations of sodium caseinate and synephrine in the basic seed culture medium were: sodium caseinate 0.15 g / L, synephrine 5 mg / L. The culture was carried out at 30°C for 7 days to obtain the mycelium of Inoculum birchii. The nutritional composition of the basic seed culture medium was: glucose 20 g / L, peptone 5 g / L, potassium dihydrogen phosphate 1.5g / L, anhydrous magnesium sulfate 1.5g / L.

[0052] (2) The inoculum of Inonotus obliquus obtained in step (1) is inoculated into a fermentation medium to which shikimic acid and sodium copper chlorophyllin are added, according to an inoculum amount of 12% of the total mass of the fermentation medium. The concentrations of shikimic acid and sodium copper chlorophyllin in the fermentation medium are: 15 mg / L shikimic acid and 1 mg / L sodium copper chlorophyllin. The culture is continued at 28° C. for 10 days to obtain a product. The nutrient composition of the fermentation medium is: 20 g / L glucose, 6 g / L beef extract, and dipotassium hydrogen phosphate. 1.5g / L, anhydrous magnesium sulfate 1.5g / L, MnSO4·H2O 0.15g / L.

[0053] (3) The product obtained in step (2) is crushed to a particle size of 5 mm, and water is added to reflux for extraction for 3 hours to obtain an extract. The extract is filtered and concentrated, and then ethanol is added to precipitate. The precipitate is collected and dried to obtain an Inonotus birchii extract.

[0054] The specific preparation process of purslane extract, aloe vera extract, sophora flavescens root extract, mint leaf extract and golden chamomile extract is the same as that of Example 1.

[0055] The preparation method of the above-mentioned moisturizing composition is as follows:

[0056] According to the above mass percentage, mix the raw materials evenly to obtain the product.

[0057] Example 3

[0058] A moisturizing composition is composed of the following raw materials in percentage by mass: 5% of Inonotus obliquus extract, 6% of Portulaca oleracea extract, 3% of Aloe vera extract, 1% of Sophora flavescens root extract, 7% of Mentha sabdariffa leaf extract, 10% of Matricaria chamomilla extract, 15% of butylene glycol, 8% of glycerol, 0.5% of 1,2-hexanediol, 0.3% of p-hydroxyacetophenone, and the balance of water.

[0059] The specific preparation process of the Inonotus obliquus extract is as follows:

[0060] (1) The inoculum strain was inoculated into a basic seed culture medium supplemented with sodium caseinate and synephrine at an inoculum amount of 8% of the total mass of the basic seed culture medium. The concentrations of sodium caseinate and synephrine in the basic seed culture medium were: sodium caseinate 0.08 g / L, synephrine 1 mg / L. The culture was carried out at 20°C for 15 days to obtain the mycelium of Inoculum birchii. The nutritional composition of the basic seed culture medium was: glucose 15 g / L, peptone 1 g / L, potassium dihydrogen phosphate 0.5g / L, anhydrous magnesium sulfate 0.5g / L.

[0061] (2) The inoculum of Inonotus obliquus obtained in step (1) was inoculated into a fermentation medium to which shikimic acid and sodium copper chlorophyllin were added, according to an inoculum amount of 8% of the total mass of the fermentation medium. The concentrations of shikimic acid and sodium copper chlorophyllin in the fermentation medium were: 9 mg / L shikimic acid and 0.4 mg / L sodium copper chlorophyllin. The culture was continued at 20° C. for 14 days to obtain a product. The nutrient composition of the fermentation medium was: 15 g / L glucose, 2 g / L beef extract, and dipotassium hydrogen phosphate. 0.5g / L, anhydrous magnesium sulfate 0.5g / L, MnSO4·H2O 0.05g / L.

[0062] (3) The product obtained in step (2) is crushed to a particle size of 5 mm, and water is added to reflux for extraction for 2 hours to obtain an extract. The extract is filtered and concentrated, and then ethanol is added for precipitation. The precipitate is collected and dried to obtain an Inonotus birchii extract.

[0063] The specific preparation process of purslane extract, aloe vera extract, sophora flavescens root extract, mint leaf extract and golden chamomile extract is the same as that of Example 1.

[0064] The preparation method of the above-mentioned moisturizing composition is as follows:

[0065] According to the above mass percentage, mix the raw materials evenly to obtain the product.

[0066] Comparative Example

[0067] Comparative Example 1

[0068] The difference between Comparative Example 1 and Example 1 is that:

[0069] In step (1) of the preparation process of the Inonotus obliquus extract, synephrine was not added to the basic seed culture medium, and the rest was the same as in Example 1.

[0070] Comparative Example 2

[0071] The difference between Comparative Example 2 and Example 1 is:

[0072] In step (2) of the preparation process of the Inonotus obliquus extract, shikimic acid was not added to the fermentation medium, and the rest was the same as in Example 1.

[0073] Comparative Example 3

[0074] The difference between Comparative Example 3 and Example 1 is that:

[0075] In step (1) of the process for preparing the Inonotus obliquus extract, shikimic acid is used instead of synephrine in the basic seed culture medium;

[0076] In step (2) of the preparation process of the Inonotus obliquus extract, synephrine is used in the fermentation medium instead of shikimic acid, and the rest is the same as in Example 1.

[0077] Test example

[0078] Test Example 1

[0079] Test Example 1 The polysaccharide content in the Inonotus obliquus extracts obtained in Examples 1-3 and Comparative Examples 1-3 was detected using the phenol-sulfuric acid method. The specific process is as follows:

[0080] 10 mg of the Inonotus obliquus extract obtained in Examples 1-3 and Comparative Examples 1-3 were respectively weighed and placed in a 50 mL volumetric flask, dissolved, diluted, and fixed to volume with ultrapure water, and shaken to obtain a sample solution of the Inonotus obliquus extract.

[0081] Take 2 mL of the sample solution from each group, immediately add 2 mL of 5% phenol solution and 10 mL of concentrated sulfuric acid to each sample solution, mix well, place in a constant temperature water bath at 80 ° C for 30 min, cool to room temperature, and then measure the absorbance of the sample at a wavelength of 486 nm. Each group was tested three times and the average value was taken. The results of the polysaccharide content in the inonotus birch extract are shown in Table 1.

[0082] Table 1

[0083]

[0084] From Table 1, it can be seen that the polysaccharide content in the Inonotus obliquus extracts obtained in Examples 1-3 is relatively high, reaching more than 35.56 wt %.

[0085] Compared with Examples 1-3, the polysaccharide content in the birch inonotus extracts obtained in Comparative Examples 1-3 was reduced to varying degrees, among which the polysaccharide content in the birch inonotus extract obtained in Comparative Example 2 was the lowest, only 18.27wt%. In the process of birch inonotus extraction, no synephrine was added to the basic seed culture medium of Comparative Example 1, no shikimic acid was added to the fermentation medium of Comparative Example 2, and the culture medium of Comparative Example 3 changed the timing of adding synephrine and shikimic acid. It can be seen from the above that in the process of birch inonotus extraction, changing the amount and timing of adding synephrine and shikimic acid will affect the content of polysaccharides in the birch inonotus extract.

[0086] The effects of adding the moisturizing compositions of Examples 1-3 and Comparative Examples 1-3 of the present invention are described below.

[0087] A skin care product, which is composed of the following raw materials in percentage by weight:

[0088] Carbomer 0.2%, PEG-40 hydrogenated castor oil 5%, behenyl alcohol 0.7%, each of the above moisturizing compositions 3%, and the balance water.

[0089] Test Example 2

[0090] The irritation of the skin to the skin of the skin care products obtained by adding the moisturizing compositions of Examples 1-3 and Comparative Examples 1-3 was tested with reference to the 2015 "Technical Specifications for Safety of Cosmetics". The specific process is as follows:

[0091] Subjects: A total of 120 subjects aged 18-45 years were selected and randomly divided into 6 groups, with a male-to-female ratio of 1:1 in each group.

[0092] Testing method: 0.02g of the skin care products obtained by adding the moisturizing compositions of Examples 1-3 and Comparative Examples 1-3 were placed in the patch tester respectively, and the patch tester with the test substance was covered on the curved side of the forearm of the test subject with a non-irritating cloth-based tape, and pressed with the palm of the hand to make it evenly adhere to the skin surface for 24 hours. After removing the test patch tester, observe the skin reaction 30 minutes after the indentation disappears. If the result is negative, observe again 48 hours after the patch test. The skin irritation test results after 48 hours are shown in Table 2.

[0093] Table 2

[0094]

[0095] It can be seen from Table 2 that the moisturizing composition provided by the present invention is mild and non-irritating to the skin and is safe to use.

[0096] Test Example 3

[0097] The moisturizing effect of the skin care products obtained by adding the moisturizing compositions of Examples 1-3 and Comparative Examples 1-3 was tested with reference to QB / T 4256-2011 "Guidelines for Evaluation of Moisturizing Efficacy of Cosmetics". The specific process is as follows:

[0098] Subjects: 60 healthy female subjects aged 18-45 years were selected and randomly divided into 6 groups.

[0099] Test environment: temperature 22℃, humidity 50%, avoid direct sunlight.

[0100] Testing method: All subjects began the test 30 minutes after entering the test environment. The subjects applied the skin care products added with the moisturizing compositions of Examples 1-3 and Comparative Examples 1-3 on their faces once in the morning and evening every day, and the skin moisture content was tested before use and 28 days after using the skin care products. Each group of data is the average value of the test results of the group.

[0101] Among them, the change rate of skin hydration content (%) = (skin hydration content after using the skin care product for 28 days - skin hydration content before use) / skin hydration content before use × 100%. See Table 3 for the results.

[0102] Table 3

[0103]

[0104] The higher the value of the skin hydration content change rate, the better the moisturizing effect of the skin care product. It can be seen from Table 3 that the composition of the present invention has an excellent moisturizing effect. Specifically, after using the skin care product added with the moisturizing composition of the present invention for 28 days, the skin hydration content change rate reaches more than 68.47%.

[0105] Compared with Examples 1 to 3, the moisturizing effect of the skin care products added with the moisturizing compositions of Comparative Examples 1 to 3 is reduced to varying degrees. Combined with the results of the polysaccharide content in the Inonotus birchii extract, the increase in polysaccharide content can promote the moisturizing effect of skin care products.

[0106] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit them. The basic principles and main features of the present invention have been described above with specific implementation schemes. On the basis of the present invention, some modifications or replacements may be made thereto, but these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the scope of protection claimed by the present invention.

Claims

1. A moisturizing composition, characterized in that: The invention is composed of the following raw materials in percentage by weight: 5-10% of Inonotus obliquus extract, 6-15% of Portulaca oleracea extract, 3-5% of Aloe vera extract, 1-3% of Sophora flavescens root extract, 7-9% of Mentha sabdariffa leaf extract, 10-12% of Matricaria recutita extract, 15-20% of Butanediol, 8-12% of Glycerin, 0.5-1% of 1,2-Hexanediol, 0.3-0.5% of p-hydroxyacetophenone, and the balance of water; The preparation process of the Inonotus obliquus extract is as follows: (1) Inonotus obliquus strains are inoculated into a basic seed culture medium supplemented with sodium caseinate and synephrine for culturing to obtain mycelium of Inonotus obliquus; (2) inoculating the mycelium of Inonotus obliquus obtained in step (1) into a fermentation medium supplemented with shikimic acid and sodium copper chlorophyll, and continuing to culture to obtain a product; (3) The product obtained in step (2) is crushed, and then water is added for reflux extraction to obtain an extract, the extract is filtered and concentrated, and then ethanol is added for precipitation, the precipitate is collected, and the precipitate is dried to obtain an Inonotus obliquus extract.

2. The moisturizing composition according to claim 1, characterized in that The concentrations of sodium caseinate and synephrine in the basic seed culture medium in step (1) are: sodium caseinate 0.08-0.15 g / L, synephrine 1-5 mg / L; the nutritional composition of the basic seed culture medium is: glucose 15-20 g / L, peptone 1-5 g / L, potassium dihydrogen phosphate 0.5-1.5 g / L, anhydrous magnesium sulfate 0.5-1.5 g / L.

3. The moisturizing composition according to claim 1, wherein The inoculation amount of the Inonotus obliquus strain in step (1) is 8-10% of the total mass of the basic seed culture medium, the culture temperature is 20-30° C., and the culture time is 7-15 days.

4. The moisturizing composition according to claim 1, wherein The concentrations of shikimic acid and sodium copper chlorophyllin in the fermentation medium in step (2) are: 9-15 mg / L shikimic acid and 0.4-1 mg / L sodium copper chlorophyllin; the nutrient composition of the fermentation medium is: 15-20 g / L glucose, 2-6 g / L beef extract, 0.5-1.5 g / L dipotassium hydrogen phosphate, 0.5-1.5 g / L anhydrous magnesium sulfate, and 0.05-0.15 g / L MnSO4·H2O.

5. The moisturizing composition according to claim 1, wherein In step (2), the inoculation amount of the inonotus obliquus mycelium is 8-12% of the total mass of the fermentation medium; the temperature of the continued culture is 20-28° C., and the time is 10-14 days.

6. The moisturizing composition according to claim 1, wherein The reflux extraction time in step (3) is 2-3 hours.

7. The method for preparing the moisturizing composition according to any one of claims 1 to 6, characterized in that: The following steps are involved: According to the said mass percentage, the raw materials are uniformly mixed to obtain the product.

8. Use of the moisturizing composition according to any one of claims 1 to 6, characterized in that: Application in the preparation of skin care products.

9. The use of the moisturizing composition according to claim 8, characterized in that: Based on the total mass of the skin care product, the mass percentage of the moisturizing composition is 1-5%.

10. The use of the moisturizing composition according to claim 8, characterized in that: The skin care product also includes the following raw materials in percentage by weight: 0.1-0.3% carbomer, 4-6% PEG-40 hydrogenated castor oil, 0.5-1% behenyl alcohol, and the balance is water.

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