Antioxidant and immune-enhancing goat milk powder for pets, preparation method and application thereof

Abstract

The present invention relates to an antioxidant immune-enhancing goat milk powder for pets, a preparation method and an application thereof, and belongs to the technical field of pet food. A selenium-rich culture medium is prepared and edible fungi are cultured to obtain selenium-rich edible fungi. Secondly, the selenium-rich edible fungi are processed into powder, and subjected to a composite enzyme and ultrasonic hydrothermal treatment to release their antioxidant and immune components to form a suspension. Finally, the suspension is ultrasonically mixed with a plant-based emulsifier, fish oil and fresh goat milk, and spray-dried into powder. This process not only retains the nutrition and digestibility of goat milk, but also significantly enhances the antioxidant and immunoregulatory functions of the product, providing comprehensive health protection for pets.

Description

Technical Field

[0001] The present application relates to the technical field of pet food, and more specifically, to an antioxidant immune-enhancing goat milk powder for pets, and a preparation method and application thereof. Background Art

[0002] With the booming modern pet economy, the pet food market is experiencing unprecedented growth, and consumers are paying more attention to pet health and nutritional needs than ever before. In this context, the development of high-quality and highly functional pet food has become an important trend in the industry. Among them, goat milk, with its unique nutritional value and superior bioavailability, has shown great application potential in the field of pet food.

[0003] As a natural functional food ingredient, goat milk is not only rich in easily digestible proteins, fats, and a variety of vitamins and minerals, but also helps maintain pet intestinal health and reduce digestive discomfort due to its near-neutral pH value and good buffering capacity. However, despite its significant advantages in nutritional digestion, goat milk itself has relatively limited functions in anti-oxidation and immune regulation, making it difficult to meet the high requirements of the modern pet food market for improving the overall health of pets.

[0004] Therefore, how to further enhance the antioxidant and immune regulation functions of goat milk while retaining its original nutritional advantages has become an important issue that needs to be urgently addressed in the field of pet food research and development. Summary of the invention

[0005] In order to solve the problems raised in the background technology, the present application provides an antioxidant immune-enhancing goat milk powder for pets and its preparation method and application. Through scientific proportioning and advanced processing technology, natural antioxidants and immune enhancers are effectively integrated into goat milk powder, in order to significantly improve its antioxidant and immune regulation capabilities while retaining its original nutritional properties, providing pets with more comprehensive and efficient health protection, and meeting the market's urgent demand for high-quality functional pet food.

[0006] The purpose of the present invention can be achieved by the following technical solutions:

[0007] A method for preparing antioxidant immune-enhancing goat milk powder for pets, specifically comprising the following steps:

[0008] S1. placing the culture medium components in deionized water, adjusting the pH value of the culture medium system to 6.5-7.0, sterilizing by high pressure, and then cooling to room temperature to obtain a selenium-enriched culture medium; then culturing edible fungi using the selenium-enriched culture medium, and then collecting fruiting bodies to obtain selenium-enriched edible fungi;

[0009] S2, cutting the selenium-rich edible fungi obtained in step S1 into pieces, then drying, grinding, and sieving to obtain selenium-rich edible fungi powder; adding the selenium-rich edible fungi powder into sterile deionized water, stirring evenly, and then treating with a composite enzyme, and then subjecting the system to ultrasonic hydrothermal treatment to obtain a selenium-rich edible fungi suspension;

[0010] S3. Ultrasonic mixing of the selenium-rich edible fungus suspension obtained in step S2, the plant-based emulsifier, fish oil and fresh goat milk, setting the ultrasonic frequency to 20-25 kHz, the treatment time to 15-30 minutes, and then spray drying to obtain an antioxidant immune-enhancing goat milk powder for pets.

[0011] Furthermore, a method for preparing antioxidant immune-enhancing goat milk powder for pets specifically comprises the following steps:

[0012] S1. Place the culture medium components in deionized water, adjust the pH value of the culture medium system to 6.5-7.0, sterilize by high pressure for 15 minutes, and then cool to room temperature to obtain a selenium-enriched culture medium; then culture the edible fungi using the selenium-enriched culture medium, collect the fruiting bodies after 5 days of culture, and obtain selenium-enriched edible fungi;

[0013] S2, cutting the selenium-rich edible fungi obtained in step S1 into pieces, then drying, grinding, and passing through an 80-mesh sieve to obtain selenium-rich edible fungi powder; adding the selenium-rich edible fungi powder into sterile deionized water at a mass volume ratio of 1 g:10 mL, stirring evenly, and then treating with a composite enzyme, and then subjecting the system to ultrasonic hydrothermal treatment to obtain a selenium-rich edible fungi suspension;

[0014] S3. Ultrasonic mixing of the selenium-rich edible fungus suspension obtained in step S2, the plant-based emulsifier, fish oil and fresh goat milk, setting the ultrasonic frequency to 20-25 kHz, the treatment time to 15-30 minutes, followed by spray drying, setting the spray flow rate to 24 mL / min, the spray drying inlet temperature to 80° C., and the outlet temperature to 50° C., to obtain an antioxidant immune-enhancing goat milk powder for pets.

[0015] Furthermore, in step S1, the bacterial inoculation amount is 3-7% (v / v), the culture temperature is 20-30° C., and the relative humidity is 60-70%.

[0016] Furthermore, in step S2, the enzyme treatment temperature is 30-50°C, and the treatment time is 10-20 minutes.

[0017] Furthermore, in step S2, the ultrasonic hydrothermal treatment temperature is 30-50°C, the ultrasonic treatment frequency is 20-80kHz, and the treatment time is 5-30 minutes.

[0018] Furthermore, the complex enzyme is obtained by mixing lipase, cellulase and pectinase in a mass ratio of 1:(2-4):(1-2).

[0019] Furthermore, the culture medium components are obtained by mixing glucose, peptone, yeast extract, KH2PO4, and sodium selenite in a mass ratio of 20:5:3:1:(0.01-0.1).

[0020] Furthermore, the plant-based emulsifier is 70 wt % ethanol extract of asparagus roots.

[0021] Furthermore, the mass ratio of the selenium-enriched edible fungus suspension, the plant-based emulsifier, the fish oil and the fresh goat milk is (10-30):(0.2-1):(5-10):100.

[0022] In summary, this application has the following beneficial effects:

[0023] (1) In the technical solution of the present invention, a composite enzyme is composed of lipase, cellulase and pectinase. Lipase is an enzyme that can hydrolyze fatty esters, which can help decompose fat in edible fungi and promote the production of short-chain fatty acids. Cellulase can decompose cellulose in the cell wall of edible fungi and release more nutrients and short-chain fatty acids. The action of pectinase can release nutrients such as the precursors of short-chain fatty acids fixed in pectin, making them easier to be absorbed and utilized by the bacteria. The three enzymes promote each other, which is beneficial to increase the content of short-chain fatty acids in the selenium-rich edible fungus system and improve the immunomodulatory effect of goat milk.

[0024] (2) In the technical solution of the present invention, the selenium-rich edible fungus liquid is first treated with a composite enzyme and then subjected to an ultrasonic hydrothermal treatment. The composite enzyme treatment can specifically act on the cell wall and cell membrane of the selenium-rich edible fungus, decompose its structural components, such as cellulose, hemicellulose and protein, etc., so that the nutrients and bioactive substances in the cells (such as selenium polysaccharides, selenoproteins, antioxidant enzymes, etc.) are more easily released. This provides a better basis for further extraction of these active substances by subsequent ultrasonic hydrothermal treatment. After the composite enzyme pretreatment, the cell structure of the selenium-rich edible fungus becomes looser, and the propagation and effect of ultrasonic waves therein will be significantly improved, and the antioxidant components and immunomodulatory factors in the selenium-rich edible fungus, such as selenium polysaccharides, selenoproteins, antioxidant enzymes, etc., can be more comprehensively extracted. The cavitation effect, mechanical effect and thermal effect of ultrasound can act more effectively on the inside of the cell, promote the release and dissolution of cell contents, thereby increasing the concentration and stability of active substances in the suspension.

[0025] (3) In the technical solution of the present invention, 70% ethanol extract of asparagus root is used as a plant-based emulsifier, which is rich in surfactants such as saponins. These substances have good emulsifying properties and can form a stable emulsion at the oil-water interface, which can accommodate short-chain fatty acids in high goat milk powder. The active ingredients such as flavonoids and saponins in the asparagus root extract also have immunomodulatory functions and can promote the immune function of goat milk powder for pets. DETAILED DESCRIPTION

[0026] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0027] The specific implementation methods of this application involve:

[0028] Lipase was provided by Sino Biotechnology Development Jiangsu Co., Ltd., CAS No. 9001-62-1, and the concentration used was 1000 U / g; cellulase and pectinase were provided by Dongheng Huadao Biotechnology Co., Ltd., and the concentration used was 1000 U / g.

[0029] The 70% ethanol extract of asparagus root used in the following examples and comparative examples was prepared by the following steps:

[0030] Asparagus roots were collected, washed with deionized water, and treated in a 90°C oven for 5 minutes. The dried asparagus roots were ground into powder using a mortar and passed through an 80-mesh sieve. The powder was extracted with a 70% ethanol solution at room temperature for 3 hours, and then filtered. The filtrate was freeze-dried to obtain a 70% ethanol extract of asparagus roots.

[0031] Example 1

[0032] A kind of anti-oxidation immunity enhancing goat milk powder for pets, the preparation method of which specifically comprises the following steps:

[0033] S1. Place the culture medium components in 1L of deionized water, adjust the pH value of the culture medium system to 6.5, then sterilize under high pressure at 120°C for 15 minutes, and then cool to room temperature to obtain a selenium-enriched culture medium; then culture the edible fungi using the selenium-enriched culture medium, control the inoculation amount of the strain to 3% (v / v), the culture temperature to 20°C, and the relative humidity to 60%, collect the fruiting bodies after 5 days of culture, and obtain selenium-enriched edible fungi;

[0034] The edible fungus used is Pleurotus ostreatus, and the variety is Xiabai No. 1; the culture medium components are 20g of glucose, 5g of peptone, 3g of yeast extract, 1g of KH2PO4, and 10mg of sodium selenite;

[0035] S2, cutting the selenium-rich edible fungi obtained in step S1 into pieces, then drying, grinding at 20°C, and passing through an 80-mesh sieve to obtain selenium-rich edible fungi powder; adding the selenium-rich edible fungi powder to sterile deionized water at a mass volume ratio of 1g:10mL, stirring evenly and treating with a composite enzyme, the mass ratio of the selenium-rich edible fungi powder to the composite enzyme is 10:1, the enzyme treatment temperature is 30°C, the treatment time is 10 minutes, and then the system is subjected to ultrasonic hydrothermal treatment, the hydrothermal treatment temperature is set to 30°C, the ultrasonic treatment frequency is 20kHz, and the treatment time is 5 minutes, to obtain a selenium-rich edible fungi suspension;

[0036] The complex enzyme is obtained by mixing lipase, cellulase and pectinase in a mass ratio of 1:2:1.

[0037] S3, ultrasonically mixing the selenium-rich edible fungus suspension obtained in step S2, the plant-based emulsifier, the fish oil and the fresh goat milk, setting the ultrasonic frequency to 20 kHz, the treatment time to 15 minutes, and then spray drying, setting the spray flow rate to 20 mL / min, the spray drying inlet temperature to 75° C., and the outlet temperature to 45° C., to obtain an antioxidant immune-enhancing goat milk powder for pets;

[0038] Among them, the plant-based emulsifier is 70% ethanol extract of asparagus root;

[0039] Among them, the mass ratio of selenium-rich edible fungus suspension, plant-based emulsifier, fish oil and fresh goat milk is 10:0.2:5:100.

[0040] Example 2

[0041] A kind of anti-oxidation immunity enhancing goat milk powder for pets, the preparation method of which specifically comprises the following steps:

[0042] S1. Place the culture medium components in 1L of deionized water, adjust the pH value of the culture medium system to 7.0, then sterilize under high pressure at 121°C for 15 minutes, and then cool to room temperature to obtain a selenium-enriched culture medium; then culture the edible fungi using the selenium-enriched culture medium, control the inoculation amount of the strain to 5% (v / v), the culture temperature to 25°C, and the relative humidity to 65%, collect the fruiting bodies after 5 days of culture, and obtain selenium-enriched edible fungi;

[0043] The edible fungus used is Pleurotus ostreatus, and the variety is Xiabai No. 1; the culture medium components are 20g glucose, 5g peptone, 3g yeast extract, 1g KH2PO4, and 30mg sodium selenite;

[0044] S2, cutting the selenium-rich edible fungi obtained in step S1 into pieces, then drying, grinding at 20°C, and passing through an 80-mesh sieve to obtain selenium-rich edible fungi powder; adding the selenium-rich edible fungi powder to sterile deionized water at a mass volume ratio of 1g:10mL, stirring evenly and treating with a composite enzyme, the mass ratio of the selenium-rich edible fungi powder to the composite enzyme is 20:1, the enzyme treatment temperature is 40°C, the treatment time is 15 minutes, and then the system is subjected to ultrasonic hydrothermal treatment, the hydrothermal treatment temperature is set to 40°C, the ultrasonic treatment frequency is 50kHz, and the treatment time is 15 minutes, to obtain a selenium-rich edible fungi suspension;

[0045] The composite enzyme is obtained by mixing lipase, cellulase and pectinase in a mass ratio of 1:3:1.5.

[0046] S3, ultrasonically mixing the selenium-rich edible fungus suspension obtained in step S2, the plant-based emulsifier, the fish oil and the fresh goat milk, setting the ultrasonic frequency to 25 kHz, the treatment time to 20 minutes, and then spray drying, setting the spray flow rate to 24 mL / min, the spray drying inlet temperature to 80° C., and the outlet temperature to 50° C., to obtain an antioxidant immune-enhancing goat milk powder for pets;

[0047] Wherein, the plant-based emulsifier is 70wt% ethanol extract of asparagus root;

[0048] Among them, the mass ratio of selenium-rich edible fungus suspension, plant-based emulsifier, fish oil and fresh goat milk is 20:0.7:7:100.

[0049] Example 3

[0050] A kind of anti-oxidation immunity enhancing goat milk powder for pets, the preparation method of which specifically comprises the following steps:

[0051] S1. Place the culture medium components in 1L of deionized water, adjust the pH value of the culture medium system to 7.0, then sterilize under high pressure at 121°C for 15 minutes, and then cool to room temperature to obtain a selenium-enriched culture medium; then culture the edible fungi using the selenium-enriched culture medium, control the inoculation amount of the strain to 7% (v / v), the culture temperature to 28°C, and the relative humidity to 70%, collect the fruiting bodies after 5 days of culture, and obtain selenium-enriched edible fungi;

[0052] The edible fungus used is black fungus, and the variety is Northeastern Auricularia auricula No. 1; the culture medium components are 20g glucose, 5g peptone, 3g yeast extract, 1g KH2PO4, and 50mg sodium selenite;

[0053] S2, cutting the selenium-rich edible fungi obtained in step S1 into pieces, then drying, grinding at 20°C, and passing through an 80-mesh sieve to obtain selenium-rich edible fungi powder; adding the selenium-rich edible fungi powder to sterile deionized water at a mass volume ratio of 1g:10mL, stirring evenly and treating with a composite enzyme, the mass ratio of the selenium-rich edible fungi powder to the composite enzyme is 30:1, the enzyme treatment temperature is 50°C, the treatment time is 20 minutes, and then the system is subjected to ultrasonic hydrothermal treatment, the hydrothermal treatment temperature is set to 50°C, the ultrasonic treatment frequency is 80kHz, and the treatment time is 30 minutes, to obtain a selenium-rich edible fungi suspension;

[0054] The composite enzyme is obtained by mixing lipase, cellulase and pectinase in a mass ratio of 1:4:2.

[0055] S3, ultrasonically mixing the selenium-rich edible fungus suspension obtained in step S2, the plant-based emulsifier, the fish oil and the fresh goat milk, setting the ultrasonic frequency to 25 kHz, the treatment time to 30 minutes, and then spray drying, setting the spray flow rate to 24 mL / min, the spray drying inlet temperature to 90° C., and the outlet temperature to 60° C., to obtain an antioxidant immune-enhancing goat milk powder for pets;

[0056] Wherein, the plant-based emulsifier is 70wt% ethanol extract of asparagus root;

[0057] Among them, the mass ratio of selenium-rich edible fungus suspension, plant-based emulsifier, fish oil and fresh goat milk is 30:1:10:100.

[0058] Comparative Example 1

[0059] A kind of anti-oxidation immunity enhancing goat milk powder for pets, the preparation method of which specifically comprises the following steps:

[0060] S1. Cut the edible fungi into pieces, dry them, grind them at 20°C, and pass them through an 80-mesh sieve to obtain edible fungi powder; add the edible fungi powder into sterile deionized water at a mass volume ratio of 1 g:10 mL, stir them evenly, and then treat them with a composite enzyme. The mass ratio of the edible fungi powder to the composite enzyme is 10:1. The enzyme treatment temperature is 30°C and the treatment time is 10 minutes. Then, the system is subjected to ultrasonic hydrothermal treatment. The hydrothermal treatment temperature is set to 30°C, the ultrasonic treatment frequency is set to 20kHz, and the treatment time is set to 5 minutes to obtain an edible fungi suspension;

[0061] The edible fungus used is Pleurotus ostreatus, and the variety is Xiabai No. 1; the complex enzyme is obtained by mixing lipase, cellulase and pectinase in a mass ratio of 1:2:1.

[0062] S2, ultrasonically mixing the edible fungus suspension obtained in step S1, the plant-based emulsifier, the fish oil and the fresh goat milk, setting the ultrasonic frequency to 20 kHz, the treatment time to 15 minutes, and then spray drying, setting the spray flow rate to 20 mL / min, the spray drying inlet temperature to 75° C., and the outlet temperature to 45° C., to obtain an antioxidant immune-enhancing goat milk powder for pets;

[0063] Among them, the plant-based emulsifier is 70% ethanol extract of asparagus root;

[0064] Among them, the mass ratio of selenium-rich edible fungus suspension, plant-based emulsifier, fish oil and fresh goat milk is 10:0.2:5:100.

[0065] Comparative Example 2

[0066] The difference between this comparative example and Example 1 is that in step S2 of this comparative example, the composite enzyme is obtained by mixing lipase and cellulase in a mass ratio of 1:2.

[0067] Comparative Example 3

[0068] The difference between this comparative example and Example 1 is that in step S2 of this comparative example, ultrasonic hydrothermal treatment is first performed and then composite enzyme treatment is performed. The specific operation is as follows:

[0069] A kind of anti-oxidation immunity enhancing goat milk powder for pets, the preparation method of which specifically comprises the following steps:

[0070] S1. Place the culture medium components in 1L of deionized water, adjust the pH value of the culture medium system to 6.5, then sterilize under high pressure at 120°C for 15 minutes, and then cool to room temperature to obtain a selenium-enriched culture medium; then culture the edible fungi using the selenium-enriched culture medium, control the inoculation amount of the strain to 3% (v / v), the culture temperature to 20°C, and the relative humidity to 60%, collect the fruiting bodies after 5 days of culture, and obtain selenium-enriched edible fungi;

[0071] The edible fungus used is Pleurotus ostreatus, and the variety is Xiabai No. 1; the culture medium components are 20g of glucose, 5g of peptone, 3g of yeast extract, 1g of KH2PO4, and 10mg of sodium selenite;

[0072] S2, cut the selenium-rich edible fungi obtained in step S1 into pieces, then dry, grind at 20°C, and pass through an 80-mesh sieve to obtain selenium-rich edible fungi powder; add the selenium-rich edible fungi powder into sterile deionized water at a mass volume ratio of 1g:10mL, stir evenly and perform ultrasonic hydrothermal treatment, setting the hydrothermal treatment temperature to 30°C, the ultrasonic treatment frequency to 20kHz, and the treatment time to 5 minutes; then use a composite enzyme treatment, the mass ratio of selenium-rich edible fungi powder to composite enzyme is 10:1, the enzyme treatment temperature is 30°C, the treatment time is 10 minutes, and then the system obtains a selenium-rich edible fungi suspension;

[0073] The complex enzyme is obtained by mixing lipase, cellulase and pectinase in a mass ratio of 1:2:1.

[0074] S3, ultrasonically mixing the selenium-rich edible fungus suspension obtained in step S2, the plant-based emulsifier, the fish oil and the fresh goat milk, setting the ultrasonic frequency to 20 kHz, the treatment time to 15 minutes, and then spray drying, setting the spray flow rate to 20 mL / min, the spray drying inlet temperature to 75° C., and the outlet temperature to 45° C., to obtain an antioxidant immune-enhancing goat milk powder for pets;

[0075] Among them, the plant-based emulsifier is 70% ethanol extract of asparagus root;

[0076] Among them, the mass ratio of selenium-rich edible fungus suspension, plant-based emulsifier, fish oil and fresh goat milk is 10:0.2:5:100.

[0077] Comparative Example 4

[0078] The difference between this comparative example and Example 1 is that the plant-based emulsifier in this comparative example is a 70 wt% ethanol extract of Quillaja bark; wherein the 70% ethanol extract of Quillaja bark is prepared by the following steps:

[0079] The soap bark was collected, washed with deionized water, and then treated in a 90°C oven for 5 minutes. The bark was ground into powder using a mortar and sieve through an 80-mesh sieve. The powder was extracted with a 70% ethanol solution at room temperature for 3 hours. The powder was then filtered and the filtrate was freeze-dried to obtain a 70% ethanol extract of the soap bark.

[0080] Comparative Example 5

[0081] The difference between this comparative example and Example 1 is that the plant-based emulsifier in this comparative example is soy protein isolate; wherein the content of the soy protein isolate is ≥95%.

[0082] Performance Testing

[0083] The goat milk powder prepared in Examples 1-3 and Comparative Examples 1-5 is now tested for its immune enhancement effect. 80 BALB / c mice weighing 20±1g were selected, and goat milk powder in different groups was fed at a dose of 9.0g / kg, three times a day, with free access to water, and then the following indicators were tested according to the method described in the Health Food Function Test and Evaluation Method (2023): ① Effect on the proliferation capacity of spleen lymphocytes; ② Effect on the activity of natural killer cells (NK cells); ③ Effect on monocyte-macrophage function. The specific test results are shown in Table 1 below.

[0084] Table 1 Test of the immune enhancement effect of goat milk powder prepared in Examples 1-3 and Comparative Examples 1-5

[0085]

[0086] From the results shown in Table 1 above, it can be seen that the immune enhancement effect of the goat milk powder prepared in Examples 1-3 of the present application is significantly better than that of the products prepared in Comparative Examples 1-5. From the results of Comparative Examples 2, 4 and 5, it can be seen that the use of the combination of lipase, cellulase and pectinase in the present invention and the use of 70wt% ethanol extract of asparagus root as an emulsifier can improve the immune enhancement effect of goat milk powder.

[0087] The goat milk powder prepared in Examples 1-3 and Comparative Examples 1-5 is now tested for its antioxidant effect. A 7mM 2,2-azidobis(3-ethylbenzothiazoline-6-sulfonic acid) aqueous solution and a 2.45mM K2S2O8 aqueous solution are placed at room temperature and kept in the dark for 12-16 hours, and the solution is diluted with a 96% ethanol solution at a volume ratio of 1:10 to obtain a diluent. 200μg of goat milk prepared in Examples 1-3 and Comparative Examples 1-5 are mixed with 3.8mL 95% ethanol solution, 1mL of diluent is added, and the mixture is stirred evenly and kept in the dark for 6 minutes. Free radicals are measured at 734nm using a spectrophotometer. Five readings are obtained for each sample and the average is taken. The test results are shown in Table 2 below.

[0088] Table 2 Test of the antioxidant effect of goat milk powder prepared in Examples 1-3 and Comparative Examples 1-5

[0089]

[0090] From the results in Table 2, it can be seen that the goat milk powder sample prepared in Example 2 has the best antioxidant effect. And the antioxidant effect of the goat milk powder prepared in Examples 1-3 of the present application is significantly better than the products prepared in Comparative Examples 1-5, that is, within the technical solution defined in the present application, the prepared goat milk powder has a better antioxidant effect.

[0091] In the description of the specification, the description with reference to the terms "one embodiment", "example", "specific example", etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representation of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner.

[0092] The above contents are merely examples and explanations of the present invention. Those skilled in the art may make various modifications or additions to the specific embodiments described or replace them in a similar manner. As long as they do not deviate from the invention or exceed the scope defined by the claims, they shall all fall within the protection scope of the present invention.

Claims

1. A method for preparing antioxidant immune-enhancing goat milk powder for pets, characterized in that: The following steps are involved: S1. placing the culture medium components in deionized water, adjusting the pH value of the culture medium system to 6.5-7.0, sterilizing by high pressure, and then cooling to room temperature to obtain a selenium-enriched culture medium; then culturing edible fungi using the selenium-enriched culture medium, and then collecting fruiting bodies to obtain selenium-enriched edible fungi; S2, cutting the selenium-rich edible fungi obtained in step S1 into pieces, then drying, grinding, and sieving to obtain selenium-rich edible fungi powder; adding the selenium-rich edible fungi powder into sterile deionized water, stirring evenly, and then treating with a composite enzyme, and then subjecting the system to ultrasonic hydrothermal treatment to obtain a selenium-rich edible fungi suspension; wherein the composite enzyme is obtained by mixing lipase, cellulase, and pectinase in a mass ratio of 1:(2-4):(1-2); S3. Ultrasonic mixing of the selenium-rich edible fungus suspension obtained in step S2, the plant-based emulsifier, fish oil and fresh goat milk, setting the ultrasonic frequency to 20-25 kHz, the treatment time to 15-30 minutes, and then spray drying to obtain an antioxidant immune-enhancing goat milk powder for pets; wherein the plant-based emulsifier is a 70wt% ethanol extract of asparagus root.

2. The method for preparing the antioxidant immune-enhancing goat milk powder for pets according to claim 1, characterized in that: In step S1, the inoculation amount of the bacterial strain is 3-7% (v / v), the culture temperature is 20-30°C, and the relative humidity is 60-70%.

3. The method for preparing the antioxidant immune-enhancing goat milk powder for pets according to claim 1, characterized in that: In step S2, the enzyme treatment temperature is 30-50°C and the treatment time is 10-20 minutes.

4. The method for preparing the antioxidant immune-enhancing goat milk powder for pets according to claim 1, characterized in that: In step S2, the ultrasonic hydrothermal treatment temperature is 30-50°C, the ultrasonic treatment frequency is 20-80kHz, and the treatment time is 5-30 minutes.

5. The method for preparing the antioxidant immune-enhancing goat milk powder for pets according to claim 1, characterized in that: In step S1, the culture medium components are obtained by mixing glucose, peptone, yeast extract, KH2PO4, and sodium selenite in a mass ratio of 20:5:3:1:(0.01-0.1).

6. The method for preparing the antioxidant immune-enhancing goat milk powder for pets according to claim 1, characterized in that: In step S3, the mass ratio of selenium-enriched edible fungus suspension, plant-based emulsifier, fish oil and fresh goat milk is (10-30):(0.2-1):(5-10):

100.

7. Goat milk powder obtained by the method for preparing antioxidant immune enhancing goat milk powder for pets as claimed in any one of claims 1 to 6.

8. Use of goat milk powder obtained by the method for preparing antioxidant immune enhancing goat milk powder for pets as claimed in any one of claims 1 to 6 in pet food.

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