A kind of polygonatum sibiricum puree and preparation method thereof

Abstract

The invention discloses a polygonatum sibiricum pulp and a preparation method thereof, and belongs to the technical field of food development. The preparation method of the polygonatum sibiricum pulp of the invention comprises the following steps: mixing polygonatum sibiricum, composite bacteria and almond powder and then fermenting to obtain a polygonatum fermented product; steaming the polygonatum fermented product and then drying it to obtain a processed polygonatum sibiricum; crushing the processed polygonatum sibiricum and mixing it with water, heating and extracting it, and filtering it to obtain a polygonatum extract; adding an adsorbent to the polygonatum extract, mixing it evenly, standing it, filtering it, and obtaining a polygonatum sibiricum pulp. The polygonatum sibiricum pulp prepared by the method of the invention has no toxicity, and without using a variety of materials to weaken some of the flavors of the polygonatum, a polygonatum sibiricum pulp with a good taste is prepared, and the antioxidant capacity of the polygonatum sibiricum pulp is improved.

Description

Technical Field

[0001] The invention relates to the technical field of food development, in particular to polygonatum sibiricum pulp and a preparation method thereof. Background Art

[0002] Polygonatum sibiricum is the dried rhizome of Polygonatum kingianum Coll.et Hemsl Delar.exRedoute, a plant of the Liliaceae family. It was first recorded in "Ming Yi Bie Lu" and is also known as "sun grass". Polygonatum sibiricum can be used for spleen and stomach weakness, fatigue, dry mouth and poor appetite, lung deficiency and dry cough, insufficient essence and blood, internal heat and thirst, etc. Polygonatum sibiricum rhizome contains a variety of nutrients, including sugar, fat, protein, starch, carotene, vitamins, etc. It can be eaten raw or stewed, which can not only satisfy hunger but also keep fit. The common Polygonatum sibiricum products on the market mainly include Polygonatum sibiricum instant powder with antioxidant effect and Polygonatum sibiricum capsules that can enhance immunity. In addition, Polygonatum sibiricum is also widely used as a food in daily diet, such as Polygonatum sibiricum porridge and Polygonatum sibiricum preserved fruit. Eating Polygonatum sibiricum directly raw has certain toxicity and causes tongue numbness, and has a bad taste. In traditional technology, it needs to be processed (such as nine steamings and nine processings) to remove the toxicity and tongue numbness. However, to completely improve the taste of the beverage, it is necessary to use a variety of ingredients to weaken the flavor of Polygonatum sibiricum itself, without adding sugar or flavorings. There are very few products that are directly prepared into a raw paste that is ready to drink. Summary of the invention

[0003] The purpose of the present invention is to provide a polygonatum sibiricum puree and a preparation method thereof, so as to solve the problems existing in the above-mentioned prior art.

[0004] To achieve the above object, the present invention provides the following solutions:

[0005] One of the technical solutions of the present invention is a method for preparing polygonatum sibiricum pulp, comprising the following steps:

[0006] The polygonatum sibiricum, the compound bacteria and the almond powder are mixed and fermented to obtain a polygonatum sibiricum fermented product;

[0007] The fermented polygonatum sibiricum is steamed and then dried to obtain the processed polygonatum sibiricum;

[0008] The processed polygonatum is crushed and mixed with water, and then heated for extraction and filtered to obtain a polygonatum extract;

[0009] The adsorbent is added to the polygonatum sibiricum extract, the mixture is evenly mixed, and then allowed to stand and filtered to obtain the polygonatum sibiricum puree.

[0010] Furthermore, the composite bacteria includes the following components, by mass: 5 to 7 parts of lactic acid bacteria, 2 to 3 parts of sake yeast and 1 to 2 parts of brewer's yeast.

[0011] The β-glucosidase produced by the compound bacteria and the β-glucosidase contained in the almond powder can decompose the polygonatum saponin in polygonatum, reducing the tongue numbness and toxicity of polygonatum. In addition, the fermentation of polygonatum by the compound bacteria can promote the dissolution of the beneficial active ingredients in polygonatum, reduce the content of bitter substances in polygonatum, and improve the taste of polygonatum.

[0012] Steaming and drying the fermented product of Polygonatum sibiricum can significantly reduce its toxicity.

[0013] The adsorbent can effectively adsorb the substances with tongue numbing feeling in the polygonatum extract and the substances that affect the taste of the polygonatum puree, effectively solving the problem of tongue numbing feeling in polygonatum and further improving the taste of polygonatum.

[0014] Furthermore, the amount of the composite bacteria is 3-4% of the mass of the polygonatum; the amount of the almond powder is 1-2% of the mass of the polygonatum;

[0015] The fermentation temperature is 32-36° C. and the fermentation time is 2-3 days.

[0016] Furthermore, the steaming temperature is 90-100° C. and the time is 8-10 hours; the drying temperature is 40° C. and the drying is performed until the moisture content is 50-55%.

[0017] Furthermore, when preparing the polygonatum sibiricum extract, the ratio of the processed polygonatum sibiricum to water is 1 g: 8-10 mL;

[0018] The temperature of the heating extraction is 100-120° C., and the time is 4-6 hours.

[0019] Furthermore, the adsorbent is modified chitosan.

[0020] Furthermore, the preparation method of the modified chitosan comprises the following steps:

[0021] Adding chitosan into acetic acid solution and dissolving it to obtain a chitosan solution; calcining shell powder and adding it into water to disperse it evenly to obtain a shell powder suspension;

[0022] Under rapid stirring, the chitosan solution is slowly added dropwise to the shell powder suspension, and after stirring for reaction, the solid product is filtered, washed and dried to obtain the modified chitosan.

[0023] Furthermore, the concentration of the acetic acid solution is 2-5wt.%; the usage ratio of the chitosan to the acetic acid solution is 2-3g:15-20mL; the usage ratio of the shell powder to water is 10-15g:100mL; the calcination temperature is 600-700°C, and the time is 4-5h;

[0024] The volume ratio of the chitosan solution to the shell powder suspension is 1:(2-3); the rotation speed of the stirring reaction is 300-400 rpm, and the time is 1-2 hours.

[0025] The second technical solution of the present invention: a polygonatum puree prepared by the above-mentioned preparation method.

[0026] The third technical solution of the present invention: an application of the above-mentioned polygonatum puree in beverage preparation.

[0027] The present invention discloses the following technical effects:

[0028] (1) The raw polygonatum sibiricum paste prepared by the method of the present invention is non-toxic. Without adding a variety of materials to weaken the taste of the raw polygonatum sibiricum, raw polygonatum sibiricum paste with good taste is prepared, and the antioxidant capacity of the raw polygonatum sibiricum paste is improved.

[0029] (2) The raw juice of Polygonatum sibiricum of the present invention does not contain any additives and can be used for people with spleen and stomach weakness, fatigue, dry mouth and poor appetite, lung deficiency and dry cough, deficiency of essence and blood, internal heat and thirst, and can be drunk for a long time. DETAILED DESCRIPTION

[0030] Various exemplary embodiments of the present invention will now be described in detail. This detailed description should not be considered as limiting the present invention, but should be understood as a more detailed description of certain aspects, features, and embodiments of the present invention.

[0031] It should be understood that the terms described in the present invention are only for describing special embodiments and are not intended to limit the present invention. In addition, for the numerical range in the present invention, it should be understood that each intermediate value between the upper and lower limits of the scope is also specifically disclosed. Each smaller range between the intermediate value in any stated value or stated range and any other stated value or intermediate value in the described range is also included in the present invention. The upper and lower limits of these smaller ranges can be independently included or excluded in the scope.

[0032] Unless otherwise indicated, all technical and scientific terms used herein have the same meanings as those generally understood by those skilled in the art. Although the present invention describes only preferred methods and materials, any methods and materials similar or equivalent to those described herein may also be used in the implementation or testing of the present invention. All documents mentioned in this specification are incorporated by reference to disclose and describe the methods and / or materials associated with the documents. In the event of a conflict with any incorporated document, the content of this specification shall prevail.

[0033] It will be apparent to those skilled in the art that various modifications and variations may be made to the specific embodiments of the present invention description without departing from the scope or spirit of the present invention. Other embodiments derived from the present invention description will be apparent to the skilled artisan. The present invention description and examples are exemplary only.

[0034] The words “include,” “including,” “have,” “contain,” etc. used in this document are open-ended terms, meaning including but not limited to.

[0035] The “parts” described in the following examples are all “parts by mass”.

[0036] The lactic acid bacteria, sake yeast, brewer's yeast and bifidobacterium used in the present invention are all commercially available. The effective viable count of lactic acid bacteria is 1.0×10 8 cfu / g; the effective viable count of sake yeast is 5.0×10 9 cfu / g; the effective viable count of Saccharomyces cerevisiae is 3.0×10 7 cfu / g; the effective viable count of Bifidobacterium is 5.0×10 9 cfu / g.

[0037] The polygonatum used in the present invention is the dried rhizome (raw polygonatum) of Polygonatum kingianum Coll.etHemsl Delar.ex Redoute, a plant of the lily family. The rhizome is nearly cylindrical or nearly bead-shaped, and the nodules are sometimes irregularly rhombus-shaped, thick, and 1 to 3 cm in diameter. The stem is 1 to 3 meters high, and the top is in a climbing shape. The leaves are arranged in whorls, with 3 to 10 leaves in each whorl, which are strip-shaped, strip-shaped wavy needle-shaped or lanceolate, 6 to 25 cm long, 3 to 30 mm wide, and fist-shaped at the tip. Inflorescence with 2-4 flowers, peduncle drooping, 1-2cm long, pedicel 0.5-1.5cm long, bracts membranous, tiny, usually located at the bottom of pedicel; perianth pink, 18-25mm long, lobes 3-5mm long; filaments 3-5mm long, filamentous or flat on both sides, anthers 4-6mm long; ovary 4-6mm long, style 10-14mm long. Berries red, 1-1.5cm in diameter, with 7-12 seeds. Flowering period March-May, fruiting period September-October.

[0038] Example 1

[0039] A method for preparing polygonatum sibiricum pulp:

[0040] (1) Cut polygonatum into slices with a thickness of about 0.5 cm, soak them in water for 20 min, take them out and mix them evenly with composite bacteria (the amount of composite bacteria is 3.5% of the mass of polygonatum) and almond powder (particle size is 100 mesh, the amount is 2% of the mass of polygonatum), ferment them at a temperature of 35° C. for 2 days, rinse them with water, and obtain a fermented polygonatum product.

[0041] Among them, the composite bacteria is composed of the following components in parts by mass: 6 parts of lactic acid bacteria, 2.5 parts of sake yeast and 2 parts of brewer's yeast.

[0042] (2) The fermented polygonatum sibiricum is steamed (steaming temperature is 100° C., time is 9 h) and then dried (temperature is 40° C.) until the water content is 52%, thereby obtaining processed polygonatum sibiricum.

[0043] (3) The processed polygonatum was crushed into 100 mesh and mixed with water (the ratio of processed polygonatum to water was 1 g: 10 mL), heated for extraction (temperature was 100° C., time was 6 h) and then filtered to obtain a polygonatum extract.

[0044] (4) Add 5 g of adsorbent (modified chitosan) to 1 L of polygonatum sibiricum extract, mix well, let stand for 2 h, and filter to obtain polygonatum sibiricum puree.

[0045] Wherein, the preparation method of modified chitosan is as follows:

[0046] 2.5 g of chitosan was added into 20 mL of 3 wt.% acetic acid solution and dissolved to obtain a chitosan solution.

[0047] The shell powder was calcined at 700°C for 5 hours, and then added into water (the ratio of shell powder to water was 10g:100mL) and dispersed evenly to obtain a shell powder suspension.

[0048] Under rapid stirring (stirring speed is 400 rpm), the chitosan solution is slowly added dropwise to the shell powder suspension (the volume ratio of the chitosan solution to the shell powder suspension is 1:3), and after stirring the reaction (speed is 400 rpm, time is 2h), the solid product is filtered, washed with water and dried (temperature is 60°C) to obtain modified chitosan.

[0049] Example 2

[0050] A method for preparing polygonatum sibiricum pulp:

[0051] (1) Cut the polygonatum into slices with a thickness of about 0.5 cm, soak them in water for 20 minutes, take them out and mix them evenly with composite bacteria (the amount of composite bacteria is 4% of the mass of the polygonatum) and almond powder (particle size is 100 mesh, the amount is 1% of the mass of the polygonatum), ferment them at a temperature of 32° C. for 2 days, rinse them with water, and obtain the polygonatum fermentation product.

[0052] Among them, the composite bacteria is composed of the following components in parts by mass: 5 parts of lactic acid bacteria, 2.5 parts of sake yeast and 1 part of brewer's yeast.

[0053] (2) The fermented polygonatum sibiricum is steamed (steaming temperature is 90° C., time is 10 h) and then dried (temperature is 40° C.) until the water content is 55%, thereby obtaining processed polygonatum sibiricum.

[0054] (3) The processed polygonatum was crushed into 100 mesh and mixed with water (the ratio of processed polygonatum to water was 1 g: 10 mL), heated for extraction (temperature was 120°C, time was 4 h) and then filtered to obtain a polygonatum extract.

[0055] (4) Add 5 g of adsorbent (modified chitosan) to 1 L of polygonatum sibiricum extract, mix well, let stand for 2 h, and filter to obtain polygonatum sibiricum puree.

[0056] Wherein, the preparation method of modified chitosan is as follows:

[0057] 2 g of chitosan was added into 15 mL of 2 wt.% acetic acid solution and dissolved to obtain a chitosan solution.

[0058] The shell powder was calcined at 600°C for 4 hours, and then added into water (the ratio of shell powder to water was 15g:100mL) and dispersed evenly to obtain a shell powder suspension.

[0059] Under rapid stirring (stirring speed is 300 rpm), the chitosan solution is slowly added dropwise to the shell powder suspension (the volume ratio of the chitosan solution to the shell powder suspension is 1:2), and after stirring the reaction (rotation speed is 300 rpm, time is 2h), the solid product is filtered, washed with water and dried (temperature is 60°C) to obtain modified chitosan.

[0060] Example 3

[0061] A method for preparing polygonatum sibiricum pulp:

[0062] (1) Cut the polygonatum into slices with a thickness of about 0.5 cm, soak them in water for 20 minutes, take them out and mix them evenly with composite bacteria (the amount of composite bacteria is 3% of the mass of the polygonatum) and almond powder (particle size is 100 mesh, the amount is 1% of the mass of the polygonatum), ferment them at a temperature of 36°C for 3 days, rinse them with water, and obtain the polygonatum fermentation product.

[0063] Among them, the composite bacteria is composed of the following components in parts by mass: 6 parts of lactic acid bacteria, 3 parts of sake yeast and 2 parts of brewer's yeast.

[0064] (2) The fermented polygonatum sibiricum is steamed (steaming temperature is 95° C., time is 8 h) and then dried (temperature is 40° C.) until the water content is 50%, thereby obtaining processed polygonatum sibiricum.

[0065] (3) The processed polygonatum was crushed into 100 mesh and mixed with water (the ratio of processed polygonatum to water was 1 g: 10 mL), heated for extraction (temperature was 100° C., time was 4 h) and then filtered to obtain a polygonatum extract.

[0066] (4) Add 5 g of adsorbent (modified chitosan) to 1 L of polygonatum sibiricum extract, mix well, let stand for 2 h, and filter to obtain polygonatum sibiricum puree.

[0067] Wherein, the preparation method of modified chitosan is as follows:

[0068] 3 g of chitosan was added into 15 mL of 5 wt.% acetic acid solution and dissolved to obtain a chitosan solution.

[0069] The shell powder was calcined at 700°C for 4 hours, and then added into water (the ratio of shell powder to water was 10g:100mL) and dispersed evenly to obtain a shell powder suspension.

[0070] Under rapid stirring (stirring speed is 350 rpm), the chitosan solution is slowly added dropwise to the shell powder suspension (the volume ratio of the chitosan solution and the shell powder suspension is 1:2.5), and after stirring the reaction (rotation speed is 350 rpm, time is 1.5 h), the solid product is filtered, washed with water and dried (temperature is 60°C) to obtain modified chitosan.

[0071] Comparative Example 1

[0072] The same as Example 1, except that the treatment in step (1) is not performed, and the treatment in step (2) is directly performed after the Polygonatum sibiricum is cut into slices with a thickness of about 0.5 cm.

[0073] Comparative Example 2

[0074] The same as Example 1, except that the sake yeast in the composite bacteria is replaced with an equal mass of Bifidobacterium.

[0075] Comparative Example 3

[0076] The same as Example 1, except that step (2) is specifically as follows: steaming the fermented polygonatum (steaming temperature is 100°C, time is 8h) and then drying (temperature is 40°C) until the water content is 52%, repeating the steaming and drying process 5 times, making the total steaming time 48h, to obtain processed polygonatum.

[0077] Comparative Example 4

[0078] Same as Example 1, except that the modified chitosan is replaced by chitosan of equal mass.

[0079] Comparative Example 5

[0080] The same as Example 1, except that the shell powder in step (4) is not calcined.

[0081] Effect Example 1

[0082] Toxicity assay:

[0083] (1) The polygonatum extract prepared in the examples and comparative examples was concentrated to a volume equivalent to 6 g of the original drug per mL (e.g., when 1 g of processed polygonatum was added to 10 mL of water for extraction, the volume of the polygonatum puree was concentrated to 0.167 mL), to obtain a polygonatum concentrate.

[0084] (2) A total of 160 ICR mice, each weighing 30-35 g, were selected. Half were male and half were female. They were divided into 8 groups, 20 mice in each group, and gavage-fed with the above-mentioned Polygonatum sibiricum concentrate, 0.5 mL per mouse each time, once in the morning, noon and evening every day for 7 consecutive days. The activity and mortality of the mice were observed. The results are shown in Table 1.

[0085] Table 1 Toxicity test results

[0086]

[0087]

[0088] Effect Example 2

[0089] Sensory evaluation:

[0090] Ten people with experience in sensory evaluation conducted a sensory evaluation on the color, smell and taste of Polygonatum sibiricum puree. The sensory scoring standards are shown in Table 2.

[0091] Table 2 Sensory scoring standards

[0092]

[0093] The sensory evaluation results are shown in Table 3.

[0094] Table 3 Sensory evaluation results

[0095]

[0096]

[0097] Effect Example 3

[0098] Determination of the antioxidant capacity of Polygonatum sibiricum puree in vitro:

[0099] (1) Preparation of DPPH working solution: Weigh an appropriate amount of DPPH, add anhydrous ethanol to dissolve and prepare a DPPH solution with a concentration of 0.05 mmol / L.

[0100] (2) The raw polygonatum sibiricum paste prepared in the examples and comparative examples was dried at low temperature (40° C.) to obtain a polygonatum sibiricum extract powder. The polygonatum sibiricum extract powder was weighed and prepared into sample solutions with concentration gradients of 2, 4, 6, 8, and 10 mg / mL with anhydrous ethanol. 0.5 mL of the sample solution of each mass concentration was transferred into a test tube, and 2 mL of the prepared DPPH solution was added to each tube. The tube was placed in a dark place for reaction for 30 min, and the absorbance value A was measured at 515 nm. i The scavenging rate of DPPH free radical can be expressed as:

[0101]

[0102] Where: A i A is the absorbance after DPPH reacts with the sample solution (0.5 mL sample solution + 2 mL DPPH solution); j is the absorbance of the sample blank (0.5 mL sample solution + 2 mL anhydrous ethanol); A0 is the DPPH solution without sample addition (0.5 mL anhydrous ethanol + 2 mL DPPH solution).

[0103] Table 4 DPPH free radical scavenging rate

[0104]

[0105]

[0106] It can be seen from the above results that the polygonatum sibiricum puree prepared in the embodiment of the present invention has good taste, good antioxidant capacity and no toxicity.

[0107] The embodiments described above are only descriptions of the preferred modes of the present invention, and are not intended to limit the scope of the present invention. Without departing from the design spirit of the present invention, various modifications and improvements made to the technical solutions of the present invention by ordinary technicians in this field should all fall within the protection scope determined by the claims of the present invention.

Claims

1. A method for preparing polygonatum sibiricum paste, characterized in that: The following steps are involved: The polygonatum sibiricum, the compound bacteria and the almond powder are mixed and fermented to obtain a polygonatum sibiricum fermented product; The fermented polygonatum sibiricum is steamed and then dried to obtain the processed polygonatum sibiricum; The processed polygonatum is crushed and mixed with water, and then heated for extraction and filtered to obtain a polygonatum extract; Adding an adsorbent to the polygonatum sibiricum extract and mixing evenly, leaving the mixture to stand, and filtering to obtain the polygonatum sibiricum puree; The composite bacteria comprises the following components, by weight: 5-7 parts of lactic acid bacteria, 2-3 parts of sake yeast and 1-2 parts of brewer's yeast; The amount of the composite bacteria is 3-4% of the mass of the polygonatum; the amount of the almond powder is 1-2% of the mass of the polygonatum; The fermentation temperature is 32-36°C and the fermentation time is 2-3 days; The adsorbent is modified chitosan; The preparation method of the modified chitosan comprises the following steps: Adding chitosan into acetic acid solution and dissolving it to obtain a chitosan solution; calcining shell powder and adding it into water to disperse it evenly to obtain a shell powder suspension; Under rapid stirring, slowly dropping the chitosan solution into the shell powder suspension, stirring for reaction, filtering the solid product, washing and drying to obtain the modified chitosan; The concentration of the acetic acid solution is 2-5wt.%; the ratio of chitosan to acetic acid solution is 2-3g:15-20mL; the ratio of shell powder to water is 10-15g:100mL; the calcination temperature is 600-700°C, and the time is 4-5h; The volume ratio of the chitosan solution to the shell powder suspension is 1:(2-3); the rotation speed of the stirring reaction is 300-400 rpm, and the time is 1-2 hours.

2. The preparation method according to claim 1, characterized in that: The steaming temperature is 90-100° C. for 8-10 hours; the drying temperature is 40° C. and the drying is performed until the moisture content is 50-55%.

3. The preparation method according to claim 1, characterized in that: When preparing the polygonatum sibiricum extract, the ratio of the processed polygonatum sibiricum to water is 1g:8-10mL; The temperature of the heating extraction is 100-120° C., and the time is 4-6 hours.

4. A polygonatum puree prepared by the preparation method according to any one of claims 1 to 3.

5. Use of the polygonatum sibiricum puree according to claim 4 in beverage preparation.

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