A detection device and detection method for Xiao'er Chaigui Tuire Granules

Abstract

The present application discloses a detection device and a detection method for pediatric chai gui tuire granules, which include a test sample preparation device for preparing a test sample solution and a control preparation device for preparing a control sample solution; the test sample preparation device includes a test sample box, a bracket arranged in the test sample box in a lifting manner, a filter screen movable on the bracket, a sampling inspection mechanism for sampling inspection of solids, and a dissolution mechanism for quickly dissolving baicalin; the sampling inspection mechanism includes a sampling inspection box arranged on the bracket, a reciprocating assembly for realizing the reciprocating flipping of the filter screen, a drying assembly for quickly drying solid particles, and a weighing assembly for weighing solid particles; the sampling inspection box is provided with a sampling inspection port, and during the flipping of the filter screen, solid particles enter the sampling inspection port, and the sampling inspection box is provided with a control assembly at the position of the sampling inspection port. The present application has the effects of good durability, small baseline noise, high sensitivity, and good peak shape; no other complex pretreatment is required during the detection process, and unnecessary interference can be avoided.

Description

Technical Field

[0001] This application relates to the field of detection of Xiao'er Chaihu Tuire Granules, and particularly to a detection device and a detection method for Xiao'er Chaigui Tuire Granules. Background Art

[0002] Currently, the main components of Xiao'er Chaigui Tuire Granules include Bupleurum chinense, Cinnamon twig, Kudzu root, Common duckweed, Skullcap root, White peony root, and Cicada slough, which have the effects of inducing sweating to relieve exterior syndrome and clearing interior heat to reduce fever, and are mainly used for infants and young children with exogenous fever, showing good effects. Due to its remarkable curative effect and small side effects, it has received praise from medical workers and patients. The current quality standard for Xiao'er Chaigui Tuire Granules is to detect the content of puerarin by high performance liquid chromatography; however, the detection of the content of baicalin is ignored. Baicalin has the effects of purging fire and removing dampness, etc., and its content is closely related to the effect of Xiao'er Chaigui Tuire Granules in treating infants and young children with exogenous fever.

[0003] The Chinese patent with the publication number CN112326846A in the related art proposed a method for detecting the content of baicalin in Xiao'er Chaigui Tuire Granules by HPLC, including preparing a test solution and a reference solution respectively, then taking 10 μl of each, injecting them into a high performance liquid chromatograph, and calculating the content of baicalin by the external standard method after detection. The chromatographic column model is Shimadzu C18, using octadecylsilane bonded silica gel as the filler, mobile phase A is methanol, mobile phase B is 0.08 - 0.12% phosphoric acid aqueous solution, and isocratic elution is carried out with A:B = 50:50 (v / v). The detection method provided by the above technical solution has good durability, low baseline noise, high sensitivity; good peak shape and small tailing factor; no other complex pretreatment is required during the detection process, which can avoid unnecessary interference; the column temperature is low, the chromatographic conditions are mild, and the operation is convenient; it has the advantages of a wide applicable concentration range, fast detection speed, high sensitivity, high precision, and good repeatability, and the detection effect is excellent.

[0004] Regarding the above related technology, the inventor believes that there are the following defects: when preparing the test sample and the reference sample, especially during the process of filtering the test sample to obtain the filtrate, the solid obtained by filtration is not analyzed. Whether there is still baicalin in the solid that has not dissolved in methanol. If there is baicalin in the solid particles, it will affect the detection of the content of baicalin. Summary of the Invention

[0005] In order to improve the problem that the presence of baicalin in the solid particles obtained after filtration affects the detection result, this application provides a detection device and a detection method for Xiao'er Chaigui Tuire Granules.

[0006] The detection device and the detection method for Xiao'er Chaigui Tuire Granules provided by this application adopt the following technical solutions:

[0007] A detection device and method for Xiao'er Chaigui Tuire Granules, including a test sample preparation device for preparing a test sample solution and a control sample preparation device for preparing a control sample solution;

[0008] The test sample preparation device includes a test sample box, a bracket that is vertically arranged in the test sample box, a filter screen that moves on the bracket, a sampling inspection mechanism for sampling inspection of solids, and a dissolving mechanism for quickly dissolving baicalin;

[0009] The sampling inspection mechanism includes a sampling inspection box arranged on the bracket, a reciprocating component for realizing the reciprocating flipping of the filter screen, a drying component for quickly drying solid particles, and a weighing component for weighing solid particles; the sampling inspection box is provided with a sampling inspection port, solid particles enter the sampling inspection port during the flipping of the filter screen, and the sampling inspection box is provided with a control component at the position of the sampling inspection port.

[0010] Optionally, the drying component includes a heating element, absorbent paper, a winding part for pulling the absorbent paper, a power part for realizing the reciprocating rotation of the sampling inspection box, and a detection part for detecting whether the water is dried. The heating element is arranged in the bottom wall of the sampling inspection box close to the absorbent paper, the absorbent paper moves along the length direction of the sampling inspection box, and the detection part and the weighing component are respectively located at both ends of the sampling inspection box.

[0011] Optionally, the power part includes a power motor and two proximity switches. The axis of rotation of the sampling inspection box connected to the bracket is located on the vertical symmetry plane of the sampling inspection box. The power motor is used to control the rotation of the sampling inspection box, and the two proximity switches are used to control the rotation angle of the sampling inspection box not to be greater than 15°.

[0012] Optionally, the detection part includes a methanol sensor, a fan, a solenoid valve, and a gas check valve. The sampling inspection box is provided with an air hole, and the sampling inspection box is provided with an air box at the position of the air hole. The air box is also provided with an air inlet hole. The solenoid valve is arranged in the air inlet hole. The fan is installed in the air box. The methanol sensor is arranged in the air box. The gas check valve is arranged at the air hole. The heating element controls the solenoid valve to work.

[0013] Optionally, the weighing component includes a weighing cylinder, a gravity sensor, and a weighing plate. The weighing cylinder and the dissolving mechanism are respectively located at both ends of the sampling inspection box. The gravity sensor is installed at the bottom end of the weighing cylinder and is used to connect the weighing plate. The gravity sensor is electrically connected to a display screen.

[0014] Optionally, the dissolution mechanism includes a dissolution cage, a driving assembly for elastically arranging the dissolution cage in the sampling box, and a shaking assembly for accelerating the dissolution of solid particles in the dissolution cage. The dissolution cage and the weighing cylinder are respectively located at two ends of the sampling box. The driving assembly includes a spring, a driving block, a first electromagnet, and a driving member. The spring is used to connect the dissolution cage and the sampling box. The driving block is fixed on the side wall of the dissolution cage. The electromagnet is used to adsorb the driving block. The driving member is used to control the reciprocating movement of the electromagnet.

[0015] Optionally, a temperature control tube is arranged inside the side wall of the dissolution cage. A phase change cavity is arranged at a position of the sampling box close to the heating member. The bottom end of the phase change cavity is open. A phase change tube is movably arranged in the phase change cavity. A traction part for pulling the phase change tube is installed in the phase change cavity. A phase change material is contained in the phase change tube. The bottom end of the phase change cavity is aligned with the top end of the temperature control tube.

[0016] Optionally, the shaking assembly includes a pull rod, a pull rope, and a non-woven fabric. The area size of the non-woven fabric is larger than the cross-sectional area of the dissolution cage. The pull rod is fixed at the center of the dissolution cage. One end of the pull rope is fixedly connected to the bottom end of the pull rod. The non-woven fabric is arranged at the bottom end of the dissolution cage.

[0017] Optionally, the control sample preparation device includes a control box, a light source arranged at the bottom of the control box, a camera arranged on any side of the control box, a light shielding mechanism for shielding other side walls of the control box, and a processing mechanism for dissolving suspended solid particles. The light shielding mechanism includes a light sensor, a plurality of light shielding plates, a plurality of light shielding ropes, a light shielding roller, and a light shielding motor. A light shielding cavity is arranged on the corresponding side wall of the control box. The light sensor is installed at the bottom of the light shielding cavity. The light sensor is electrically connected to the light shielding motor. Adjacent light shielding plates are connected by the light shielding ropes.

[0018] A detection method for Xiao'er Chaigui Tuire Keli provided by the present application adopts the following technical solution:

[0019] A detection method for Xiao'er Chaigui Tuire Keli includes the following steps:

[0020] S1. Preparation of the test solution: Weigh Xiao'er Chaigui Tuire Keli and dissolve it in a methanol aqueous solution. After ultrasonic treatment, filter it, and take the filtrate to obtain the test solution; and conduct a sampling inspection on the filtered solid particles to ensure that the solid particles do not contain baicalin.

[0021] S2. Preparation of the reference solution: Accurately weigh the baicalin reference substance, add it to a methanol aqueous solution, stir well and make up the volume. Ensure that the baicalin is completely dissolved in methanol through light detection to obtain a reference solution with a known concentration.

[0022] S3. HPLC determination: Take 10 μl of the test solution and the reference solution respectively, inject them into a high-performance liquid chromatograph, and calculate the content of baicalin by the external standard method after detection;

[0023] In step S3, the chromatographic column model is Shimadzu C18, and octadecylsilane-bonded silica gel is used as the filler; mobile phase A is methanol, and mobile phase B is 0.08 - 0.12% phosphoric acid aqueous solution, with isocratic elution of A:B = 50:50 (v / v).

[0024] In summary, this application includes at least one of the following beneficial technical effects:

[0025] 1. When the bracket moves above the liquid level, it is necessary to move the solid particles on the filter screen into the sampling inspection box. At the initial state, the end of the filter screen is in contact with the side wall of the sampling inspection box. As the reciprocating member controls the filter screen to start rotating, at this time, the filter screen tilts towards the sampling inspection box. The set rotation angle of the filter screen is less than 10°. That is, the gap between the filter screen and the sampling inspection box can be ignored, and most of the solid particles can fall into the sampling inspection box through the sampling port. At this time, the torsion spring is in a deformed state, and the reciprocating electromagnet is powered off. Under the action of the torsion spring force, the filter screen returns to the initial state. Under the action of this movement, the solid particles above the filter screen can be turned over, greatly accelerating the movement of the solid particles. And the reciprocating electric push rod also returns to the initial position under the action of gravity and then controls the reciprocating electromagnet to continue to adsorb the filter screen. After repeating the above operation multiple times, the solid particles on the filter screen are moved into the sampling inspection box;

[0026] 2. When the solid particles enter the sampling inspection box, at this time, control the sampling inspection box to swing reciprocally and control the solid particles to move reciprocally on the blotting paper. The blotting paper adsorbs methanol. After the adsorption work is carried out for a period of time, at this time, the heating element can be turned on to work. The blotting paper is pulled into the winding cavity, and through the cooperation of two winding rollers, the winding rope is pulled to the bottom wall of the sampling inspection box. The heating element starts to heat the methanol on the solid particles and evaporate it. Since there may be risks in methanol heating, it is necessary to turn on the fan and the solenoid valve, and control the heating temperature of the heating element. After the methanol evaporates, it is pumped into the air box by the fan. If there is methanol, the methanol sensor controls the buzzer through the PLC controller. When the methanol sensor detects methanol vapor, at this time, the buzzer is triggered to start working, and the heating element stops working. It is still necessary to continue to absorb methanol from the solid particles until there is no methanol;

[0027] 3. The camera starts to take pictures of the methanol solution in the control box. If there are small suspended particles in the control box, it proves that baicalin has been dissolved. If there are more suspended particles in the control box, it is necessary to continue to operate to dissolve baicalin in the methanol solution. First, control the position of the filter element to move towards the methanol liquid level until the protective rod is inserted into the insertion hole and the second electromagnet adsorbs the protective rod. At this time, control the rotating rod to rotate through the processing motor, and the protective plate on the protective rod moves to the position of the filter plate. Continue to lift the filter element, and the methanol and the suspended matter both move to a position far away from the control box. At this time, quickly control the rotating rod to rotate, and the methanol will flow out from the position of the filter plate. However, since the protective plate moves fast, the time for stirring and dissolving the methanol and baicalin is sufficient. If suspended matter is still found, repeat the above operations multiple times to ensure that baicalin can be fully dissolved in methanol, and complete the preparation of the solution in the control box. Description of the Drawings

[0028] Figure 1 is a schematic diagram of the pilot sample preparation device in the first embodiment of the present application;

[0029] Figure 2 is a partial structure schematic diagram in the first embodiment of the present application;

[0030] Figure 3 is a schematic diagram of the sampling inspection mechanism and the dissolving mechanism in the first embodiment of the present application;

[0031] Figure 4 is Figure 3 the enlarged view of A in

[0032] Figure 5 is a schematic diagram of the control sample preparation device in the first embodiment of the present application;

[0033] Figure 6 is a schematic diagram of the filter element in the first embodiment of the present application;

[0034] Figure 7 is a schematic diagram of the protective rod and the protective plate in the first embodiment of the present application.

[0035] Reference numerals: 1, test sample box; 2, bracket; 3, filter screen; 4, sampling inspection box; 5, sampling inspection port; 6, control board; 7, reciprocating member; 8, reciprocating block; 9, reciprocating electromagnet; 10, heating member; 11, blotting paper; 12, winding roller; 13, winding rope; 14, power motor; 15, methanol sensor; 16, fan; 17, solenoid valve; 18, gas check valve; 19, gas box; 20, air inlet hole; 21, air hole; 22, weighing cylinder; 23, gravity sensor; 24, weighing plate; 25, dissolution cage; 26, spring; 27, driving block; 28, first electromagnet; 29, driving member; 30, temperature control tube; 31, phase change cavity; 32, phase change tube; 33, towing rope; 34, towing roller; 35, pull rod; 36, pull rope; 37, non-woven fabric; 38, control box; 39, light source; 40, camera; 41, light sensor; 42, light-shielding plate; 43, light-shielding rope; 44, light-shielding roller; 45, light-shielding cavity; 46, filtering member; 47, protective rod; 48, protective plate; 49, rotating rod; 50, second electromagnet; 51, processing motor; 52, filter plate; 53, solid plate. Detailed implementation manners

[0036] The following further elaborates on this application Figure 1-7 in conjunction with the appended drawings. Embodiment 1

[0037] The embodiment of this application discloses a detection device for Xiao'er Chaigui Tuire Keli. Refer to Figures 1-4, A detection device for Xiao'er Chaigui Tuire Granules includes a test sample preparation device for preparing a test sample solution and a control sample preparation device for preparing a control sample solution; during the determination using the HPLC method, it is necessary to prepare a test sample and a control sample. The test sample preparation device mainly ensures that baicalin can be completely dissolved in methanol and will not remain in solid particles, while the control sample preparation device mainly needs to ensure that the baicalin material can be quickly dissolved in the methanol solution; the test sample preparation device includes a test sample box 1, a bracket 2 arranged to be lifted in the test sample box 1, a filter screen 3 moving on the bracket 2, a sampling inspection mechanism for sampling and inspecting solids, and a dissolution mechanism for quickly dissolving baicalin; the lifting of the bracket 2 can be achieved by an electric push rod or a cylinder. The filter screen 3 is rotatably arranged on the bracket 2, and the rotation axis of the filter screen 3 connected to the bracket 2 is located on the symmetry plane of the filter screen 3. The sampling inspection mechanism is used to detect the solid particles filtered by the filter screen 3. In this embodiment, multiple groups of tests are carried out simultaneously to ensure the accurate detection of the baicalin content. However, during the multiple groups of tests, it is not necessary to sample and inspect whether there is still baicalin in the solid particles for each group of tests. Therefore, the sampling inspection mechanism is used to determine whether there is still baicalin in the solid particles generated in a certain group; the dissolution mechanism is to dissolve the solid particles filtered for the first time again. If the weight of the solid particles does not change after dissolution, it proves that there is no excess baicalin in the solid particles. If the weight of the solid particles changes after dissolution, it proves that there is excess baicalin in the solid particles, and the baicalin content detection work needs to be carried out again after dissolving the solid particles multiple times.

[0038] The sampling inspection mechanism includes a sampling inspection box 4 arranged on the bracket 2, a reciprocating component for realizing the reciprocating flipping of the filter screen 3, a drying component for quickly drying the solid particles, and a weighing component for weighing the solid particles; the sampling inspection box 4 is fixed at any end perpendicular to the rotation axis of the filter screen 3. The sampling inspection box 4 is provided with a sampling inspection port 5, and during the rotation of the filter screen 3, the solid particles enter the sampling inspection box 4 from the sampling inspection port 5, that is, the sampling inspection port 5 is lower than the plane where the filter screen 3 is located, and the sampling inspection box 4 is located at the lower end during the rotation of the filter screen 3. The sampling inspection box 4 is provided with a control component at the position of the sampling inspection port 5. The control component includes a control board 6 and an electric push rod. The control board 6 moves along the vertical direction, and the electric push rod is installed in the sampling inspection box 4. In this embodiment, a protection box needs to be installed in the sampling inspection box 4 to protect the electric push rod. The control board 6 is used to control the opening and closing of the sampling inspection port 5.

[0039] The reciprocating component includes a reciprocating member 7, a reciprocating block 8, a reciprocating electromagnet 9, and a torsion spring. The housing of the reciprocating member 7 is connected to the bracket 2, and the output end of the reciprocating member 7 is also hinged to the reciprocating block 8. The reciprocating electromagnet 9 is fixed on the reciprocating block 8 and is used to adsorb one end of the filter screen 3 away from the sampling box 4. The two ends of the torsion spring are respectively fixed to the filter screen 3 and the bracket 2. When the filter screen 3 is in a horizontal state, the torsion spring is in its original state at this time. When the bracket 2 moves above the liquid level, it is necessary to move the solid particles on the filter screen 3 into the sampling box 4. And in the initial state, the end of the filter screen 3 is in contact with the side wall of the sampling box 4. Both ends of the filter screen 3 are set to be arc-shaped to facilitate the swing of the filter screen 3. When the reciprocating member 7 controls the filter screen 3 to start rotating, the filter screen 3 tilts towards the sampling box 4 at this time. In this embodiment, since there is a gap between the corresponding end of the filter screen 3 and the side wall of the sampling box 4 during the rotation of the filter screen 3, it is set that the rotation angle of the filter screen 3 is less than 10°, that is, the gap between the filter screen 3 and the sampling box 4 can be ignored, and most of the solid particles can fall into the sampling box 4 from the sampling port 5. At this time, the torsion spring is in a deformed state, and the reciprocating electromagnet 9 is powered off. Under the action of the elastic force of the torsion spring, the filter screen 3 returns to the initial state. And under the action of this movement of the filter screen 3, the solid particles above it can be turned over, greatly accelerating the movement of the solid particles. And the reciprocating electric push rod also returns to the initial position under the action of gravity and then controls the reciprocating electromagnet 9 to continue to adsorb the filter screen 3. After repeating the above operation multiple times, the solid particles on the filter screen 3 are moved into the sampling box 4.

[0040] The drying component includes a heating element 10, a blotting paper 11, a winding part for pulling the blotting paper 11, a power part for realizing the reciprocating rotation of the sampling box 4, and a detection part for detecting whether the water is dried. The power part includes a power motor 14 and two proximity switches. The axis of rotation of the sampling box 4 connected to the bracket 2 is located on the vertical symmetry plane of the sampling box 4. The power motor 14 is used to control the rotation of the sampling box 4, and the two proximity switches are used to control the rotation inside the sampling box 4. The proximity switches are connected to the power motor 14 through a PLC controller. The two proximity switches respectively correspond to the two ends of the sampling box 4, that is, when the power motor 14 controls the rotation of the sampling box 4, when the higher end of the sampling box 4 moves to a position close to the proximity switch, the rotation angle of the sampling box 4 is less than 10° at this time. The purpose of setting 10° is mainly to prevent solid particles from moving to both ends. At this time, the solid particles only roll in the middle position of the sampling box 4 and will not roll to the two ends of the sampling box 4. Similarly, when the other end of the sampling box 4 rotates to the corresponding proximity switch, the proximity switch is also triggered at this time, and the proximity switch controls the power motor 14 to rotate in the other direction.

[0041] The heating element 10 is arranged on the bottom wall of the sampling box 4 close to the blotting paper 11. The width dimension of the blotting paper 11 is adapted to the width dimension of the sampling box 4. The blotting paper 11 moves along the length direction of the sampling box 4. The detection part and the weighing component are respectively located at both ends of the sampling box 4. Therefore, in this application, two proximity switches are used to ensure that the solid particles roll on the blotting paper 11. The blotting paper 11 is used to absorb methanol from the solid particles. Methanol can be absorbed by the blotting paper 11 because of the combination of the liquid state of methanol and the water absorption of the blotting paper 11, so that methanol molecules can be adsorbed by the fiber structure of the blotting paper 11 and penetrate into its interior. The heating element 10 can be an electric heating wire. The sampling box 4 is provided with a heating cavity at a position close to the test paper. The heating element 10 is installed in the heating cavity. The winding part includes two winding rollers 12, a plurality of winding ropes 13 and two winding motors. The sampling box 4 is provided with a winding cavity at a position close to the heating cavity. The winding rollers 12 rotate in the winding cavity. The plurality of winding ropes 13 are arranged at intervals with the plurality of blotting papers 11. The winding ropes 13 at both ends are respectively fixedly connected to the circumferences of the two winding rollers 12. The winding motor is used to control the rotation of the winding rollers 12. The heating cavity is provided with two through grooves for the winding paper to pass through. The through holes are adapted to the blotting paper 11. In this embodiment, it is set that the area of the blotting paper 11 is larger than the dimension between the two through grooves. Therefore, a part of the blotting paper 11 is located in the through grooves, and thus the probability of methanol entering the through grooves can be greatly reduced.

[0042] Refer to Figures 1-4, the detection unit includes a methanol sensor 15, a fan 16, a solenoid valve 17, and a gas check valve 18. The sampling box 4 is provided with an air hole 21. An air box 19 is installed at the position of the air hole 21 of the sampling box 4. The fan 16 and the methanol sensor 15 are installed in the air box 19. The air box 19 is also provided with an air inlet hole 20. The solenoid valve 17 is installed in the air inlet hole 20. The fan 16 is installed in the air box 19. The methanol sensor 15 is arranged in the air box 19. The gas check valve 18 is arranged at the air hole 21. The heating element 10 controls the operation of the solenoid valve 17. The heating element 10 and the solenoid valve 17 work through a PLC controller. When solid particles enter the sampling box 4, at this time, the sampling box 4 is controlled to swing reciprocally and the solid particles are controlled to move reciprocally on the blotting paper 11. The blotting paper 11 adsorbs methanol. After the adsorption work is carried out for a period of time, at this time, the heating element 10 can be turned on to work. The blotting paper 11 is pulled into the winding cavity, and through the cooperation of two winding rollers 12, the winding rope 13 is pulled to the bottom wall of the sampling box 4. The heating element 10 starts to heat the methanol on the solid particles and make it evaporate. Since there may be risks in methanol heating, it is necessary to turn on the fan 16 and the solenoid valve 17, and control the heating temperature of the heating element 10. After the methanol evaporates, it is drawn into the air box 19 by the fan 16. If there is methanol, the methanol sensor 15 is connected to a buzzer through a PLC controller. When the methanol sensor 15 detects methanol vapor, at this time, the buzzer is triggered to start working, and the heating element 10 stops working. It is still necessary to continue to absorb methanol from the solid particles until there is no methanol left.

[0043] Refer to Figures 1-4 , the weighing assembly includes a weighing cylinder 22, a gravity sensor 23, and a weighing plate 24. When the methanol absorption work is completed, it is necessary to weigh the solid particles. The weighing cylinder 22 and the dissolution mechanism are respectively located at both ends of the sampling box 4. The gravity sensor 23 is installed at the bottom end of the weighing cylinder 22 and is used to connect the weighing plate 24. The gravity sensor 23 is electrically connected to a display screen. The gravity sensor 23 and the display screen are controlled and connected through a PLC controller. The power motor 14 controls the sampling box 4 to rotate from a horizontal state to a vertical state and controls the weighing cylinder 22 to be at the bottom. The weighing plate 24 is used to receive the solid particles. At this time, the gravity sensor 23 displays the weight of the solid particles at this time through the display screen, and the staff reads and records it.

[0044] The dissolution mechanism includes a dissolution cage 25, a driving component for elastically setting the dissolution cage 25 in the sampling box 4, and a shaking component for accelerating the dissolution of solid particles in the dissolution cage 25. The dissolution cage 25 and the weighing cylinder 22 are respectively located at both ends of the sampling box 4. The driving component includes a spring 26, a driving block 27, a first electromagnet 28, and a driving member 29. A sliding rod is fixedly connected to the dissolution cage. A sliding hole for the sliding of the sliding rod is provided on the inner wall of the sampling box 4. The two ends of the spring 26 are respectively fixedly connected to one end of the sliding rod and the inner wall of the end of the sliding hole, achieving the effect of elastic connection between the dissolution cage 25 and the sampling box 4. The driving block 27 is fixed on the side wall of the dissolution cage 25. The electromagnet is used to adsorb the driving block 27, and the driving member 29 is used to control the reciprocating movement of the electromagnet. The driving member 29 can adopt an electric push rod or a cylinder. And in this embodiment, the driving member 29 also needs to be provided with a protective box for protection; in the initial state, the electromagnet adsorbs the driving block 27, and the spring 26 is in a deformed state. When solid particles enter the dissolution cage 25, the aperture on the dissolution cage 25 is smaller than the size of the solid particles. Therefore, the solid particles will only dissolve in the methanol solution and will not fall out of the dissolution cage 25. Before the first electromagnet 28 is powered off, the position of the bracket 2 needs to be controlled to ensure that the dissolution cage 25 can enter the methanol solution after being finally released. Then, the first electromagnet 28 can be powered off. Under the elastic force of the spring 26, the dissolution cage 25 is ejected into the methanol solution, and the methanol starts to dissolve the solid particles again. And because the dissolution cage 25 is ejected into the methanol solution, the solid particles are also suspended in the methanol solution under the impact of methanol from the inner wall of the dissolution cage 25, greatly accelerating the dissolution work.

[0045] Refer to Figures 1-4, a temperature control tube 30 is arranged inside the side wall of the dissolution cage 25. A phase change cavity 31 is arranged in the sampling inspection box 4 near the heating element 10. The bottom end of the phase change cavity 31 is open. A phase change tube 32 is movably arranged inside the phase change cavity 31. A traction part for pulling the phase change tube 32 is installed inside the phase change cavity 31. The phase change tube 32 is filled with a phase change material. The bottom end of the phase change cavity 31 is aligned with the top end of the temperature control tube 30. The length direction of the heating cavity is perpendicular to the moving direction of the winding rope 13, while the length direction of the phase change cavity 31 is arranged parallel to the length direction of the heating cavity. The traction part includes a traction motor, a traction roller 34 and a traction rope 33. The traction motor is fixed at the top end of the phase change cavity 31. The traction roller 34 is rotatably connected to the top end of the phase change cavity 31. One end of the traction rope 33 is fixedly connected to the peripheral wall of the traction roller 34. During the whole process, the phase change tube 32 is always in a vertical state. Before the dissolution cage 25 is separated from the sampling inspection box 4, the phase change tube 32 is controlled to gradually fall. The phase change tube 32 moves into the temperature control tube 30, and it is necessary to ensure that the winding rope 13 is in a relaxed state to prevent the phase change tube 32 from falling out of the temperature control tube 30. When the phase change tube 32 is inside the phase change cavity 31, it starts to absorb the heat of the heating element 10. When the dissolution cage 25 enters the methanol solution, at this time the phase change tube 32 starts to release heat, and it quickly heats the methanol solution inside and near the dissolution cage 25. If baicalin exists in the solid particles, the dissolution rate of baicalin can be accelerated.

[0046] Refer to Figures 1-4 , the jitter assembly includes a pull rod 35, a pull rope 36 and a non-woven fabric 37. The area size of the non-woven fabric is larger than the cross-sectional area of the dissolution cage 25. The pull rod 35 is fixed inside the sampling inspection box 4 and is located at the center of the dissolution cage 25. One end of the pull rope 36 is fixedly connected to the bottom end of the pull rod 35. The non-woven fabric 37 is arranged at the bottom end of the dissolution cage 25. The non-woven fabric 37 is in a natural hanging state in the initial state. When the dissolution cage 25 impacts into the methanol solution, most of the solid particles will float towards the methanol solution. In order to make all the solid particles float towards the methanol solution, as the dissolution cage 25 continues to move towards the methanol solution, at this time the non-woven fabric 37 is quickly pulled upwards by the pull rope 36. The non-woven fabric 37 has a jittering force. At this time, all the solid particles are jittered from the non-woven fabric 37 into the methanol solution, further accelerating the dissolution speed of the solid particles in the methanol. After repeating the above operation, after pulling the dissolution cage 25 out of the water, the sampling inspection box 4 is rotated to a horizontal position, and after adsorbing the methanol with the blotting paper 11, the dissolved solid particles are weighed by the gravity sensor 23. If the weight changes, then baicalin exists in the solid particles; if the weights of the two weighings do not change, then baicalin does not exist in the normal solid particles.

[0047] Refer to Figures 5-7, the control sample device includes a control box 38, a light source 39 disposed at the bottom of the control box 38, a camera 40 disposed on either side of the control box 38, a light-shielding mechanism for shielding the other side walls of the control box 38, and a treatment mechanism for dissolving suspended solid particles. First, the light-shielding mechanism shields the side walls of the control box 38, and the light source 39 is turned on. If there are suspended substances in the methanol solution, it proves that baicalin has not been completely dissolved, and the treatment mechanism is used to accelerate the dissolution of baicalin; the light-shielding mechanism includes a light sensor 41, a plurality of light-shielding plates 42, a plurality of light-shielding ropes 43, a light-shielding roller 44, and a light-shielding motor. A light-shielding cavity 45 is provided on the corresponding side wall of the control box 38. The light sensor 41 is disposed at the bottom of the light-shielding cavity 45 and is used to identify the light of the light source 39. The material of the control box 38 is made of a transparent material. Adjacent light-shielding plates 42 are connected by light-shielding ropes 43. The light sensor 41 is electrically connected to the light-shielding motor. The light sensor 41 and the light-shielding motor are controlled and connected through a PLC controller. After the light sensor 41 recognizes the light of the light source 39, the light-shielding motor starts to work. The light-shielding motor starts to control the light-shielding roller 44 to start rotating. The light-shielding roller 44 starts to continuously lower the light-shielding plates 42 it winds. The light-shielding plates 42 start to shield the side walls of the control box 38 to reduce the influence of ambient light on the shooting of the camera 40. The camera 40 starts to shoot the methanol solution in the control box 38. If there are relatively small suspended particles in the control box 38, it proves that baicalin has been dissolved. If there are more suspended particles in the control box 38, it is necessary to continue to operate to dissolve baicalin in the methanol solution.

[0048] Refer to Figures 5-7The processing mechanism includes a filter element 46 that is lifted and arranged in the control box 38, a protective rod 47 that is rotatably arranged in the filter element 46, a plurality of protective plates 48 fixed on the outer peripheral wall of the protective rod 47, a rotating rod 49, a second electromagnet 50 and a processing motor 51. The lifting and lowering of the filter element 46 is realized by an electric push rod or a cylinder. The protective rod 47 rotates at the center position of the filter element 46. The processing motor 51 is installed on the top of the control box 38 and is used to control the rotation of the rotating rod 49. The rotating rod 49 is provided with a plug-in hole for plugging in the protective rod 47. In this embodiment, the protective rod 47 and the plug-in hole are The cross-sections are all set to be square, and the second electromagnet 50 is installed on the top inner wall of the plug hole. The second electromagnet 50 is used to adsorb the protective rod 47, that is, when the protective rod 47 is plugged into the plug hole, the protective rod 47 can be driven by the rotating rod 49 to rotate together. The protective plate 48 is arranged at equal intervals on the outer peripheral wall of the protective rod 47. The filter 46 includes a plurality of filter plates 52 and a plurality of solid plates 53. In this embodiment, the solid plates 53 and the filter plates 52 are set to three in number, so the number of the protective plates 48 is also set to three. The filter plate 52 is provided with a plurality of filter holes for filtering baicalin. Filtering, while no filter holes are set on the solid plate 53, the protective plate 48 corresponds to the solid plate 53, and the bottom of the protective plate 48 is close to the top of the solid plate 53, and the size of the protective plate 48 is larger than the size of the filter plate 52, so as to ensure that when the protective plate 48 moves to the position of the filter plate 52, the methanol moves with the filter element 46; when the light finds that there are more suspended matter in the control box 38, it is necessary to accelerate the dissolution of the suspended matter. In this embodiment, the filter element 46 is first controlled to move toward the position of the methanol liquid surface until the protective rod 47 is plugged into the plug hole and the second electromagnet 50 is used to drive the protective rod 47. Adsorption is carried out, at this time, the processing motor 51 is used to control the rotation of the rotating rod 49, and the protective plate 48 on the protective rod 47 moves to the filter plate 52 position, and the filter element 46 continues to be lifted. Methanol follows the suspended matter and moves to a position away from the control box 38. At this time, the rotating rod 49 is quickly controlled to rotate, and methanol will flow out from the filter plate 52 position. However, since the protective plate 48 moves quickly, the time for stirring and dissolving methanol and baicalin is sufficient. If suspended matter is still found, the above operation is repeated for multiple times to ensure that baicalin can be fully dissolved in methanol and complete the preparation of the solution in the control box 38.

[0049] The implementation principle of a detection device for pediatric chai gui tuire granules in an embodiment of the present application is as follows: When the support 2 moves above the liquid level, it is necessary to move the solid particles on the filter screen 3 into the sampling box 4. And in the initial state, the end of the filter screen 3 is attached to the side wall of the sampling box 4. As the reciprocating member 7 controls the filter screen 3 to start rotating, at this time, the filter screen 3 is inclined towards the sampling box 4. It is set that the rotation angle of the filter screen 3 is less than 10°, that is, the gap between the filter screen 3 and the sampling box 4 can be ignored. Most of the solid particles can fall into the sampling box 4 from the sampling port 5. At this time, the torsion spring is in a deformed state, and the reciprocating electromagnet 9 is powered off. Under the action of the torsion spring elastic force, the filter screen 3 returns to the initial state. And under the action of this movement of the filter screen 3, the solid particles above it can be turned over, greatly accelerating the movement of the solid particles. And the reciprocating electric push rod also returns to the initial position under the action of gravity and then controls the reciprocating electromagnet 9 to continue to adsorb the filter screen 3. After repeating the previous operation multiple times, the solid particles on the filter screen 3 are moved into the sampling box 4.

[0050] When the solid particles enter the sampling box 4, at this time, control the sampling box 4 to swing reciprocally and control the solid particles to move reciprocally on the blotting paper 11. The blotting paper 11 adsorbs methanol. After the adsorption work is carried out for a period of time, at this time, the heating member 10 can be turned on to work. The blotting paper 11 is pulled into the winding cavity, and through the cooperation of the two winding rollers 12, the winding rope 13 is pulled to the bottom wall of the sampling box 4. The heating member 10 starts to heat the methanol on the solid particles and make it evaporate. Since there may be risks in methanol heating, it is necessary to turn on the fan 16 and the solenoid valve 17, and control the heating temperature of the heating member 10. After the methanol evaporates, it is pumped into the gas box 19 by the fan 16. If there is methanol, the methanol sensor 15 is connected to a buzzer through the PLC controller. When the methanol sensor 15 detects methanol vapor, at this time, the buzzer is triggered to start working, and the heating member 10 stops working. It is also necessary to continue to absorb methanol from the solid particles until there is no methanol.

[0051] In the initial state, the first electromagnet 28 adsorbs to the driving block 27, and the spring 26 is in a deformed state. When solid particles enter the dissolution cage 25, in the initial state, the first electromagnet 28 adsorbs to the driving block 27, and the spring 26 is in a deformed state. When solid particles enter the dissolution cage 25, at this time, the phase change tube 32 starts to release heat, and rapidly heats the methanol solution inside and near the dissolution cage 25. If baicalin exists in the solid particles, the dissolution rate of baicalin can be accelerated. As the dissolution cage 25 continues to move into the methanol solution, at this time, the non-woven fabric 37 is rapidly pulled upward by the pulling rope 36. The non-woven fabric 37 has a jolting force, and at this time, all the solid particles are jolted from the non-woven fabric 37 into the methanol solution, further accelerating the dissolution rate of the solid particles in methanol; after repeating the above operations, after pulling the dissolution cage 25 out of the water, rotate the sampling inspection box 4 to the horizontal position, and after adsorbing the methanol with the absorbent paper 11, weigh the dissolved solid particles with the gravity sensor 23. If the weight changes, then baicalin exists in the solid particles; if the weights of the two weighings do not change, then baicalin does not exist in the normal solid particles.

[0052] The camera 40 starts to photograph the methanol solution in the control box 38. If there are smaller suspended particles in the control box 38, it proves that the baicalin has been dissolved. If there are more suspended particles in the control box 38, the operation of dissolving baicalin in the methanol solution needs to be continued. First, control the filter element 46 to move towards the position of the methanol liquid level until the protective rod 47 is inserted into the insertion hole and the second electromagnet 50 adsorbs the protective rod 47. At this time, control the rotating rod 49 to rotate through the processing motor 51. The protective plate 48 on the protective rod 47 moves to the position of the filter plate 52, and continue to lift the filter element 46. The methanol and the suspended matter both move to a position far from the control box 38. At this time, start to quickly control the rotating rod 49 to rotate, and the methanol will flow out from the position of the filter plate 52. However, because the moving speed of the protective plate 48 is fast, the time for stirring and dissolving the methanol and baicalin is sufficient. If suspended matter is still found, repeat the above operations multiple times to ensure that the baicalin can be fully dissolved in the methanol, and complete the preparation of the solution in the control box 38. Example Two

[0053] This application example discloses a detection method for Xiao'er Chaigui Tuire Granules. Refer to Figure 1 , a detection method for Xiao'er Chaigui Tuire Granules includes the following steps:

[0054] S1. Preparation of test solution: Weigh Xiao'er Chaigui Tuire Keli and dissolve it in a methanol-water solution. After ultrasonic treatment, filter it, and the filtrate is the test solution. Additionally, randomly inspect the solid particles after filtration to ensure that they do not contain baicalin. By redissolving the filtered solid particles and comparing whether there is a weight change after the two dissolutions, it is determined whether the solid particles contain baicalin. If the random inspection process proves that the solid particles contain baicalin, the test sample needs to be prepared again to ensure the accuracy of the baicalin content detection.

[0055] S2. Preparation of reference solution: Weigh accurately the reference substance of baicalin, add it to a methanol-water solution, stir well and make up the volume. Ensure that the baicalin is completely dissolved in methanol through light detection, and then the reference solution with a known concentration is obtained. And if the baicalin is not fully dissolved, the suspended particles are taken out and stirred and dissolved again to greatly improve the solubility and dissolution efficiency of baicalin.

[0056] S3. Determination by HPLC method: Take 10 μl of the test solution and the reference solution respectively, inject them into a high-performance liquid chromatograph, and calculate the baicalin content by the external standard method after detection.

[0057] In step S3, the chromatographic column model is Shimadzu C18, and octadecylsilane chemically bonded silica gel is used as the packing material. Mobile phase A is methanol, and mobile phase B is 0.08 - 0.12% phosphoric acid aqueous solution, with isocratic elution of A:B = 50:50 (v / v). Using the above-mentioned chromatographic column model can better separate baicalin from other components in Xiao'er Chaigui Tuire Keli, with a good peak shape and a small tailing factor. This type of chromatographic column uses octadecylsilane chemically bonded silica gel as the packing material, which can achieve excellence and durability and obtain a good chromatographic peak shape.

[0058] The above are all preferred embodiments of this application, and the protection scope of this application is not limited thereby. Therefore, all equivalent changes made according to the structure, shape, and principle of this application should be covered within the protection scope of this application.

Claims

1. A detection device for children's Chaigui antipyretic granules, characterized by: It includes a sample preparation device for preparing a test solution and a control sample preparation device for preparing a reference solution; The sample preparation device comprises a sample box (1), a support (2) which is lifted and arranged in the sample box (1), a filter (3) which is movable on the support (2), a sampling mechanism for sampling solids, and a dissolving mechanism for quickly dissolving baicalin; The sampling inspection mechanism comprises a sampling inspection box (4) arranged on the support (2), a reciprocating component for realizing the reciprocating flipping of the filter screen (3), a drying component for quickly drying solid particles, and a weighing component for weighing solid particles; the sampling inspection box (4) is provided with a sampling inspection port (5), and solid particles enter the sampling inspection port (5) during the flipping process of the filter screen (3); the sampling inspection box (4) is provided with a control component at the position of the sampling inspection port (5); The drying component comprises a heating element (10), absorbent paper (11), a winding part for pulling the absorbent paper (11), a power part for realizing the reciprocating rotation of the sampling inspection box (4), and a detection part for detecting whether the water is dried, the heating element (10) being arranged in the bottom wall of the sampling inspection box (4) close to the absorbent paper (11), the absorbent paper (11) moving along the length direction of the sampling inspection box (4), and the detection part and the weighing component being respectively located at two ends of the sampling inspection box (4); The detection unit comprises a methanol sensor (15), a fan (16), a solenoid valve (17) and a gas one-way valve (18); the sampling inspection box (4) is provided with an air hole (21); the sampling inspection box (4) is provided with an air box (19) at the position of the air hole (21); the air box (19) is also provided with an air inlet (21); the solenoid valve (17) is arranged in the air inlet (20); the fan (16) is installed in the air box (19); the methanol sensor (15) is arranged in the air box (19); the gas one-way valve (18) is arranged at the air hole (21); and the heating element (10) controls the operation of the solenoid valve (17); The weighing assembly comprises a weighing cylinder (22), a gravity sensor (23) and a weighing plate (24); the weighing cylinder (22) and the dissolving mechanism are respectively located at two ends of the sampling box (4); the gravity sensor (23) is installed at the bottom end of the weighing cylinder (22) and is used to connect to the weighing plate (24); the gravity sensor (23) is electrically connected to a display screen.

2. The pediatric Chaigui antipyretic granule detection device according to claim 1 is characterized in that: The power unit comprises a power motor (14) and two proximity switches. The axis of the rotational connection between the sampling box (4) and the bracket (2) is located on the vertical symmetry plane of the sampling box (4). The power motor (14) is used to control the rotation of the sampling box (4). The two proximity switches are used to control the rotation angle of the sampling box (4) to be no greater than 15°.

3. The detection device for Xiaoer Chaigui Antipyretic Granules according to claim 2 is characterized in that: The dissolution mechanism includes a dissolution cage (25), a driving component for realizing the elastic setting of the dissolution cage (25) in the sampling box (4), and a shaking component for accelerating the dissolution of solid particles in the dissolution cage (25). The dissolution cage (25) and the weighing cylinder (22) are respectively located at two ends of the sampling box (4). The driving component includes a spring (26), a driving block (27), a first electromagnet (28) and a driving member (29). The spring (26) is used to connect the dissolution cage (25) and the sampling box (4). The driving block (27) is fixed to the side wall of the dissolution cage (25). The first electromagnet (28) is used to adsorb the driving block (27). The driving member (29) is used to control the reciprocating motion of the first electromagnet (28).

4. The detection device for Xiaoer Chaigui Antipyretic Granules according to claim 3 is characterized in that: A temperature control tube (30) is arranged in the side wall of the dissolution cage (25), and a phase change chamber (31) is arranged in the sampling box (4) at a position close to the heating element (10). The bottom end of the phase change chamber (31) is opened, and a phase change tube (32) is movably arranged in the phase change chamber (31). A traction part for pulling the phase change tube (32) is installed in the phase change chamber (31), and a phase change material is installed in the phase change tube (32). The bottom end of the phase change chamber (31) is aligned with the top end of the temperature control tube (30).

5. The detection device for Xiaoer Chaigui Antipyretic Granules according to claim 4 is characterized in that: The shaking assembly includes a pull rod (35), a pull rope (36) and a non-woven fabric (37). The area of ​​the non-woven fabric (37) is larger than the cross-sectional area of ​​the dissolving cage (25). The pull rod (35) is fixed at the center of the dissolving cage (25). One end of the pull rope (36) is fixedly connected to the bottom end of the pull rod (35). The non-woven fabric (37) is arranged at the bottom end of the dissolving cage (25).

6. The detection device for Xiaoer Chaigui Antipyretic Granules according to claim 5 is characterized in that: The control sample preparation device comprises a control box (38), a light source (39) arranged at the bottom of the control box (38), a camera (40) arranged on either side of the control box (38), a shading mechanism for shading other side walls of the control box (38), and a processing mechanism for dissolving suspended solid particles. The shading mechanism comprises a light sensor (41), a plurality of shading plates (42), a plurality of shading ropes (43), a shading roller (44) and a shading motor. A shading cavity (45) is arranged on the corresponding side wall of the control box (38). The light sensor (41) is installed at the bottom of the shading cavity (45). The light sensor (41) is electrically connected to the shading motor. Adjacent shading plates (42) are connected via the shading ropes (43).

7. A method for detecting Chaigui Antipyretic Granules for Children, based on the Chaigui Antipyretic Granules for Children detection device according to any one of claims 1 to 6, characterized in that: The steps include: S1. Preparation of the test solution: weigh the Xiaoer Chaigui Tuire Granules and dissolve them in a methanol-water solution, filter them after ultrasonic treatment, and take the filtrate to obtain the test solution; and perform random inspection on the filtered solid particles to ensure that the solid particles do not contain baicalin; S2. Preparation of reference solution: accurately weigh baicalin reference substance, add it to methanol-water solution, stir well and make up the volume, and ensure that baicalin is completely dissolved in methanol by light detection, so as to obtain a reference solution of known concentration; S3. HPLC determination: Take 10 μl of the test solution and the reference solution respectively, inject them into the high performance liquid chromatograph, and calculate the baicalin content by the external standard method after detection; In step S3, the chromatographic column model is Shimadzu C18, and octadecylsilane bonded silica gel is used as filler; the mobile phase A is methanol, and the mobile phase B is 0.08-0.12% phosphoric acid aqueous solution, and the elution is isocratic with A:B=50:50 (v / v).

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