Neomealybug neo-attractant and application thereof

Abstract

The invention discloses an attractant for neo-mealybug nereis and application of the attractant, and the attractant is prepared from the following components in parts by weight: 5 to 6 parts of 3-hexanone, 10 to 12 parts of 3-hexanol, 10 to 12 parts of 2-hexanol, 2 to 3 parts of 3-ethyl acetophenone and 2 to 3 parts of 2, 2-bis (2-tetrahydrofuryl) propane. The neo-mealybug attractant can be applied to prevention and control of the neo-mealybug attractant, and negative effects of chemical prevention and control on crops such as sisal hemp, pineapples, pumpkins, bananas and mangosteen can be reduced.

Description

Technical Field

[0001] The invention relates to the technical field of prevention and control of new pineapple gray mealybugs, in particular to a new pineapple gray mealybug attractant and application thereof. Background Art

[0002] Dysmicoccus neobrevipes is an invasive pest in my country, belonging to the genus Dysmicoccus of the family Pseudococcidae of the order Hemiptera. The insect threatens 76 species of tropical and subtropical agricultural and forestry economic crops from 39 families, including sisal, pineapple, pumpkin, banana and mangosteen, and is a fierce player that harms sisal, fruits, vegetables and flowers in the breeding areas. The insect not only sucks nutrients from the host, but also spreads a variety of plant viruses, such as sisal purple leafroll disease and pineapple wilt disease, causing significant economic losses.

[0003] At present, the prevention and control of the new pineapple gray mealybug is mainly chemical control, but the insect has strong reproductive capacity, high outbreak frequency, strong concealment and evolving resistance to pesticides, which greatly increases the difficulty of prevention and control. Therefore, how to safely and effectively control the damage of the new pineapple gray mealybug and explore new ways to control the insect has become an urgent problem to be solved.

[0004] Attractants are active substances produced by plants or artificially synthesized that have behavioral attractant effects on specific insects. They are effective biological control agents and are widely used in the production of organic agricultural products such as rice, corn, cotton, fruit trees, and vegetables. However, there are currently few reports on the control of new pineapple gray mealybugs. Summary of the invention

[0005] The invention discloses a new pineapple gray mealybug attractant and application thereof. The new pineapple gray mealybug attractant can be applied to the prevention and treatment of the new pineapple gray mealybug attractant, which is beneficial to reducing the negative impact of chemical prevention and treatment on crops such as sisal, pineapple, pumpkin, banana and mangosteen.

[0006] To achieve the above object, the technical solution of the present invention is: A new pineapple gray mealybug attractant is composed of the following components in parts by weight: 5-6 parts of 3-hexanone, 10-12 parts of 3-hexanol, 10-12 parts of 2-hexanol, 2-3 parts of 3-ethylacetophenone and 2-3 parts of 2,2-di(2-tetrahydrofuranyl)propane.

[0007] Preferably, the new pineapple gray mealybug attractant described above is composed of the following components in parts by weight: 5 parts of 3-hexanone, 10 parts of 3-hexanol, 10 parts of 2-hexanol, 2.5 parts of 3-ethylacetophenone, and 2.5 parts of 2,2-di(2-tetrahydrofuranyl)propane.

[0008] The present invention also provides application of the above-mentioned new pineapple gray mealybug attractant in preventing and controlling new pineapple gray mealybug.

[0009] Furthermore, the attractant is mixed with a carrier, or adsorbed into a carrier, or sprayed on weeds in a sisal field.

[0010] Furthermore, the carrier is one of rubber, polyethylene sustained-release bottle, liquid paraffin and activated carbon.

[0011] Furthermore, the concentration of the attractant in liquid paraffin is 10-1000 ng / μL.

[0012] The invention is based on the study of volatiles of the host of the new pineapple gray mealybug, and obtains an attractant containing a combination of 3-hexanone, 1,3-hexanol, 2-hexanol, 3-ethylacetophenone and 2,2-di(2-tetrahydrofuranyl)propane. The attractant has obvious attracting effect on the new pineapple gray mealybug, can be applied to the prevention and control of the new pineapple gray mealybug attractant, and is beneficial to reducing the negative impact of chemical control on crops such as sisal, pineapple, pumpkin, banana and mangosteen. BRIEF DESCRIPTION OF THE DRAWINGS

[0013] Figure 1 The figures are the attracting effects of Examples 1-4 and Comparative Examples 1-3 on male Pseudococcus spp.

[0014] Figure 2 The diagram shows the attracting effect of Examples 1-4 and Comparative Examples 1-3 on female new pineapple gray mealybugs. DETAILED DESCRIPTION

[0015] The present invention is further described below in conjunction with specific embodiments, but the protection scope of the present invention is not limited to the following embodiments.

[0016] The 3-hexanone, 3-hexanol and 2-hexanol used in this example were collected from pineapple by dynamic headspace adsorption, and 3-ethylacetophenone and 2,2-di(2-tetrahydrofuranyl)propane were collected from sisal by dynamic headspace adsorption. The components were determined by comparison with standard samples, which were purchased from Shanghai MacLean Biochemical Technology Co., Ltd. The liquid paraffin oil used was analytical grade, S10187, SCRC. Example

[0017] A new pineapple gray mealybug attractant is composed of the following components in parts by weight: 5 parts of 3-hexanone, 10 parts of 3-hexanol, 10 parts of 2-hexanol, 2.5 parts of 3-ethylacetophenone and 2.5 parts of 2,2-di(2-tetrahydrofuranyl)propane.

[0018] Prepare 1 μg / μL 3-hexanone solution, 1 μg / μL 3-hexanol solution, 1 μg / μL 2-hexanol solution, 1 μg / μL 3-ethylacetophenone solution, and 1 μg / μL 2,2-di(2-tetrahydrofuranyl)propane solution respectively, and use liquid paraffin as the dilution solvent. Stir until the mixture is uniform, and then dilute to prepare 10 ng / μL attractant solution, and the carrier of the attractant is liquid paraffin. Example

[0019] Example 2 is basically the same as Example 1, but is finally diluted to prepare an attractant solution of 100 ng / μL, and the carrier of the attractant is liquid paraffin. Example

[0020] Example 2 is basically the same as Example 1, but is finally diluted to prepare an attractant solution of 1000 ng / μL, and the carrier of the attractant is liquid paraffin. Example

[0021] A new pineapple gray mealybug attractant is composed of the following components in parts by weight: 6 parts of 3-hexanone, 10 parts of 3-hexanol, 10 parts of 2-hexanol, 2 parts of 3-ethylacetophenone and 2 parts of 2,2-di(2-tetrahydrofuranyl)propane.

[0022] Prepare 1 μg / μL 3-hexanone solution, 1 μg / μL 3-hexanol solution, 1 μg / μL 2-hexanol solution, 1 μg / μL 3-ethylacetophenone solution, and 1 μg / μL 2,2-di(2-tetrahydrofuranyl)propane solution respectively, and use liquid paraffin as the dilution solvent. Stir until the mixture is uniform, and then dilute to prepare 10 ng / μL attractant solution, and the carrier of the attractant is liquid paraffin.

[0023] Comparative Example 1 The attractant consisted of 3-hexanol and 2-hexanol in a weight ratio of 1:1.

[0024] Prepare 1 μg / μL 3-hexanol solution and 1 μg / μL 2-hexanol solution respectively, use liquid paraffin as the dilution solvent, stir until the mixture is uniform, and then dilute with liquid paraffin to prepare a 10 ng / μL attractant solution.

[0025] Comparative Example 2 The attractant is composed of 3-hexanone and 3-ethylacetophenone in a weight ratio of 2:1.

[0026] Prepare 1 μg / μL 3-hexanone and 1 μg / μL 3-ethylacetophenone respectively, use liquid paraffin as the dilution solvent, stir until the mixture is uniform, and then dilute with liquid paraffin to prepare an attractant solution of 10 ng / μL.

[0027] Comparative Example 3 The attractant was prepared by diluting 2,2-di(2-tetrahydrofuryl)propane with liquid paraffin to prepare an attractant solution of 10 ng / μL.

[0028] The Y-shaped olfactometer was used to measure the tropism response of the new pineapple gray mealybug to the attractant, and the new pineapple gray mealybugs with intact antennae and relatively active behavior were selected for testing. In each group of sample tests, the number of new pineapple gray mealybugs was 100, and females and males were tested separately.

[0029] Cut the filter paper strips into 5cm×1cm, fold them into a "V" shape, drop the samples of Examples 1-4 and Comparative Examples 1-3 into them, and put them into the flavor source bottle on one side. On the other side, drop 20μl of liquid paraffin on the filter paper as a control. The "Y"-shaped tube was placed in a dark box and the temperature was controlled at 26℃.

[0030] Each time, 10 insects were tested. They were connected from the tube mouth. After 5 minutes, the behavioral response of the new pineapple gray mealybugs to the odor source was observed. When the new pineapple gray mealybugs crawled to 1 / 2 of a certain arm and stopped for more than 30 seconds, it was considered to have made a choice for the odor. If it still did not make a choice after 5 minutes, it was considered to have no choice and the result was recorded. After testing 10 insects, the left and right directions of the "Y"-shaped tube were reversed to eliminate the influence of the environment on the test results; after testing 20 insects, they were cleaned with acetone, and then the inner wall of the olfactometer was wiped with cotton wool soaked in ethanol, rinsed with distilled water, and dried in an oven. The above operation was regarded as one repetition, and each treatment was repeated 5 times, and females and males were tested separately.

[0031] Figure 1 The figures are the attracting effects of Examples 1-4 and Comparative Examples 1-3 on male Pseudococcus spp. Figure 2 The figure is a diagram showing the attracting effect of Example 1-4 and Comparative Example 1-3 on female new pineapple gray mealybugs. It can be seen from the figure that the attractant of this embodiment has an obvious attracting effect on the new pineapple gray mealybugs, especially when the concentration is 10ng / μL, the attracting effect is the best, and the attracting effect on female new pineapple gray mealybugs is better.

Claims

1. A new pineapple mealybug attractant, characterized in that: The invention is composed of the following components in parts by weight: 5-6 parts of 3-hexanone, 10-12 parts of 3-hexanol, 10-12 parts of 2-hexanol, 2-3 parts of 3-ethylacetophenone and 2-3 parts of 2,2-di(2-tetrahydrofuranyl)propane.

2. The new pineapple mealybug attractant according to claim 1, wherein: The invention is composed of the following components in parts by weight: 5 parts of 3-hexanone, 10 parts of 3-hexanol, 10 parts of 2-hexanol, 2.5 parts of 3-ethylacetophenone, and 2.5 parts of 2,2-di(2-tetrahydrofuryl)propane.

3. Application of the new pineapple gray mealybug attractant according to any one of claims 1-2 in preventing and controlling the new pineapple gray mealybug.

4. The use according to claim 3, characterized in that: The attractant is mixed with a carrier, or adsorbed in the carrier, or sprayed on weeds in a sisal field.

5. The use according to claim 4, characterized in that: The carrier is one of rubber, polyethylene sustained-release bottle, liquid paraffin and activated carbon.

6. The use according to claim 5, characterized in that: The concentration of the attractant in liquid paraffin is 10-1000 ng / μL.

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