An anti-wrinkle composition, its preparation method and use

Abstract

The application belongs to the technical field of cosmetics, and discloses an anti-wrinkle composition, a preparation method and application thereof. The composition comprises the following components: mevalonolactone, hydroxytyrosol, guaiacol sulfonate sodium and betaine. The application utilizes the superior antioxidant performance of hydroxytyrosol, combines mevalonolactone which can promote the synthesis of cholesterol, and guaiacol sulfonate sodium which has excellent anti-inflammatory effect, controls from multiple aspects of free radical elimination, cholesterol synthesis to maintain skin elasticity, and inhibition of the release of inflammatory factors and inflammatory mediators, and simultaneously cooperates with betaine which is easy to penetrate and has a calming effect. Through compounding the four components, the synergistic effect can be exerted, so that excellent anti-inflammatory, wrinkle-removing and anti-aging effects can be achieved.

Description

TECHNICAL FIELD

[0001] The present application belongs to the technical field of cosmetics, and particularly relates to an anti-wrinkle composition, a preparation method and application thereof. BACKGROUND

[0002] The skin is affected by many factors such as external environment or self metabolism, and free radicals are formed. When the free radicals destroy the collagen and active substances in the normal cell membrane tissue, wrinkles are formed. With the growth of age, metabolism slows down, water and subcutaneous fat decrease, and elastic fibers gradually become thicker, so the skin becomes more wrinkled. Skin inflammation can activate some enzymes present in the dermis, such as metal matrix proteinases, which can decompose collagen fibers, hyaluronic acid, mucopolysaccharides and other substances in the dermis, resulting in large pores, wrinkles and loose skin, which is commonly referred to as "inflammatory aging". Inflammation can also damage the supporting tissues of the skin, elastin and skin barrier function, thereby accelerating skin aging.

[0003] Inflammation is closely related to skin aging, and inflammation is considered an important factor in skin aging. At present, most of the anti-aging products on the market only start from the perspective of removing free radicals or supplementing or promoting the growth of collagen, but few researches are made from the perspective of anti-aging from multiple aspects such as anti-inflammatory. SUMMARY

[0004] The present application aims to at least solve one of the technical problems existing in the prior art. To this end, the present application provides an anti-wrinkle composition, a preparation method and application thereof, which can restore skin elasticity, promote fibroblast proliferation, reduce or remove intracellular free radicals, inhibit the production of inflammatory cells and certain cytokines, and reduce capillary permeability, and has the effects of significant anti-inflammatory, restoring skin elasticity and improving skin tightness.

[0005] In a first aspect of the present application, a composition is provided, which comprises the following components: methylvaleronate lactone, hydroxytyrosol, sodium guaiacol sulfonate and betaine.

[0006] The following is an introduction to the components in the composition of the present application and the related mechanism:

[0007] Mevastatin is a component discovered in the process of studying sake, which is extracted from the filtrate of Sacchromycopsis Fibuligera after biological culture. It is widely present in many animals, plants and microorganisms in nature and participates in many biological synthesis processes. Mevalonolactone is extracted from Sacchromycopsis Fibuligera and is a natural anti-aging component with an optical degree of 100%. Mevalonolactone is hydrolyzed into mevalonic acid (mevalonic acid) in the skin, which participates in the cell metabolic pathway and promotes cholesterol synthesis, which is important for maintaining the normal function of the skin. With age, the synthesis decreases, resulting in insufficient cholesterol synthesis in the skin. Cholesterol is also an important component of skin cells, which helps maintain skin elasticity and moisture content. Maintaining moderate cholesterol levels is also important for maintaining the health and youth of the skin.

[0008] Hydroxytyrosol is a natural polyphenolic compound with strong antioxidant activity, which can also promote wound healing and eliminate free radicals. Hydroxytyrosol can promote skin cell metabolism, enhance skin elasticity and moisture, reduce wrinkles and anti-aging, and also has anti-inflammatory effects, which can reduce skin inflammation and swelling.

[0009] Sodium guaiazolyl sulfonate is an effective component extracted from chrysanthemum (chrysanthemum and chamomile), which has excellent anti-inflammatory, anti-allergic and tissue repair effects. It is currently a substance with dual effects of targeting anti-inflammatory and tissue regeneration in the field of mucosal anti-inflammatory repair. It mainly has the effects of anti-allergy, anti-inflammatory, mucosal repair and prevention of ulcers. It should be noted that the use and dosage of sodium guaiazolyl sulfonate should be determined according to the specific situation, and it cannot be used arbitrarily.

[0010] Betaine is a kind of alkaloid with the chemical name of N,N,N-trimethyl glycine. It is mainly used as a moisturizing agent in cosmetics. This ingredient easily interacts with water to produce moisturizing ability. Betaine has excellent moisturizing ability and does not have a sticky feeling when used. It can make the skin smoother after use, and its combination with other ingredients can also be used to reduce skin allergies, making it an ideal soothing moisturizer. In addition, it can also balance cell osmotic pressure and help cells restore balance and moisturizing state when the skin encounters harsh environments and cell osmotic pressure is imbalanced and cells are dehydrated.

[0011] Immune aging, cell aging and light exposure are the inducing factors of inflammatory aging. During the process of immune aging, immune cells such as T cells and macrophages change, and the change of macrophages is a target that skin care products can aim to improve. Specifically, macrophages are generally divided into two polarization types: M1 type promoting inflammation and M2 type resisting inflammation. The secretion of M1 type macrophages can cause the increase of the proportion of fibroblast aging, and also can inhibit the synthesis of collagen and promote the decomposition of collagen, so that the collagen fiber is significantly reduced. The number of fibroblasts in the human body gradually decreases, and the collagen and elastin secreted by the fibroblasts are gradually reduced and broken due to oxidation, so the support to the epidermis disappears, causing uneven collapse, the dermis layer begins to thin, the skin elasticity becomes poor, and wrinkles begin to gradually produce. Therefore, inflammation plays an important role in skin aging. After many research tests, the four components of methylvalerate lactone, hydroxytyrosol, guaiacol sodium sulfonate and betaine are screened from many raw material components in the field, and the four components have a synergistic effect after compounding. They play a role in many aspects such as scavenging free radicals, synthesizing cholesterol to maintain skin elasticity, and inhibiting the release of inflammatory factors and inflammatory mediators, and excellent anti-inflammatory, wrinkle removal and anti-aging effects are achieved.

[0012] In some embodiments of the present application, the composition comprises the following components by weight: 0.01-0.5 parts of methylvalerate lactone, 0.001-0.05 parts of hydroxytyrosol, 0.001-0.05 parts of guaiacol sodium sulfonate and 0.1-5 parts of betaine.

[0013] In some embodiments of the present application, the composition comprises the following components by weight: 0.01-0.1 parts of methylvalerate lactone, 0.001-0.01 parts of hydroxytyrosol, 0.001-0.01 parts of guaiacol sodium sulfonate and 0.1-1 parts of betaine.

[0014] In some embodiments of the present application, the composition comprises the following components by weight: 0.1-0.5 parts of methylvalerate lactone, 0.01-0.05 parts of hydroxytyrosol, 0.01-0.05 parts of guaiacol sodium sulfonate and 1-5 parts of betaine.

[0015] In the second aspect of the present application, a preparation method of the composition of the first aspect of the present application is provided, comprising the following steps:

[0016] The components are mixed to obtain the composition.

[0017] In the third aspect of the present application, an external skin preparation is provided, which comprises the composition of the first aspect of the present application.

[0018] In some embodiments of the present application, the composition is present in the external skin preparation in an amount of 0.1-6 wt%, for example, can be any of 0.1%, 0.5%, 1%, 2%, 3%, 4%, 5%, 6%, or a range between any two of them.

[0019] In a fourth aspect of the present application, a cosmetic product is provided, which comprises the composition according to the first aspect of the present application.

[0020] In some embodiments of the present application, the composition is present in the cosmetic product in an amount of 0.1-6 wt%, for example, can be any of 0.1%, 0.5%, 1%, 2%, 3%, 4%, 5%, 6%, or a range between any two of them.

[0021] In some embodiments of the present application, the cosmetic product further comprises a cosmetically acceptable adjuvant.

[0022] In some embodiments of the present application, the adjuvant comprises at least one of a humectant, a thickening agent, a chelating agent, an emulsifying agent, an emollient, a pH adjusting agent, a preservative, and a fragrance.

[0023] In some embodiments of the present application, the cosmetic product comprises any of an essence, a lotion, a cream, or a mask.

[0024] Compared with the prior art, the present application has the following advantages:

[0025] The present application utilizes the superior antioxidant properties of hydroxytyrosol, combines with methylvaleronate lactone which can promote the synthesis of cholesterol, and sodium guaiacol sulfonate which has excellent anti-inflammatory effect, to control from multiple aspects of free radical scavenging, cholesterol synthesis to maintain skin elasticity, and inhibition of the release of inflammatory factors and inflammatory mediators, while synergizing with betaine which is easy to penetrate and has a calming effect. By compounding the four components, a synergistic effect can be achieved, thereby achieving excellent anti-inflammatory, wrinkle removal, and anti-aging effects. BRIEF DESCRIPTION OF DRAWINGS

[0026] Figure 1 A comparison chart of eye wrinkles before and after using an eye cream with the composition of Example 10. DETAILED DESCRIPTION

[0027] In order to make those skilled in the art more clearly understand the technical solutions of the present application, the following examples are given for illustration. It should be pointed out that the following examples do not constitute a limitation on the scope of protection required by the present application.

[0028] The raw materials, reagents or devices used in the present application can be obtained from conventional commercial channels, or can be obtained by existing known methods, unless otherwise specified.

[0029] The raw material information used in the embodiments of the present application is as follows:

[0030] Methylvalonic acid lactone is from Aidi Fine Chemical (Zhejiang) Co., Ltd.;

[0031] Hydroxytyrosol is from Maple Yang Biological Research and Development (Nanjing) Co., Ltd.;

[0032] Sodium guaiacol sulfonate is from Suobang (Huzhou) Biological Medicine Technology Co., Ltd.;

[0033] Betaine is from Nanjing Lihong Biological Technology Co., Ltd.

[0034] Examples 1-10

[0035] The compositions in Examples 1-10 use the components in Table 1 and their proportions.

[0036] Table 1 Composition of Examples 1-10 and their proportions by weight

[0037]

[0038] The compositions in Examples 1-10 were prepared by mixing the components in proportions according to Table 1.

[0039] Comparative Example 1

[0040] The composition in this example differs from Example 10 only in that it does not contain methylvalonic acid lactone, and the other components and amounts are the same as in Example 10.

[0041] Comparative Example 2

[0042] The composition in this example differs from Example 10 only in that it does not contain hydroxytyrosol, and the other components and amounts are the same as in Example 10.

[0043] Comparative Example 3

[0044] The composition in this example differs from Example 10 only in that it does not contain sodium guaiacol sulfonate, and the other components and amounts are the same as in Example 10.

[0045] Comparative Example 4

[0046] The composition in this example differs from Example 10 only in that it does not contain betaine, and the other components and amounts are the same as in Example 10.

[0047] Comparative Example 5

[0048] The composition in this example differs from Example 10 only in that it does not contain methylvalonic acid lactone and hydroxytyrosol, and the other components and amounts are the same as in Example 10.

[0049] Comparative Example 6

[0050] The composition in this example differs from Example 10 only in that it does not contain methylvalerate lactone and sodium gualenate, and the other components and amounts are the same as in Example 10.

[0051] Comparative Example 7

[0052] The composition in this example differs from Example 10 only in that it does not contain methylvalerate lactone and betaine, and the other components and amounts are the same as in Example 10.

[0053] Comparative Example 8

[0054] The composition in this example differs from Example 10 only in that it does not contain hydroxytyrosol and sodium gualenate, and the other components and amounts are the same as in Example 10.

[0055] Comparative Example 9

[0056] The composition in this example differs from Example 10 only in that it does not contain hydroxytyrosol and betaine, and the other components and amounts are the same as in Example 10.

[0057] Comparative Example 10

[0058] The composition in this example differs from Example 10 only in that it does not contain sodium gualenate and betaine, and the other components and amounts are the same as in Example 10.

[0059] Comparative Example 11

[0060] The composition in this example differs from Example 10 only in that it contains an equivalent amount of a similar efficacy filtrate of fermentate of schizochytrium sp. instead of methylvalerate lactone, and the other components and amounts are the same as in Example 10.

[0061] Comparative Example 12

[0062] The composition in this example differs from Example 10 only in that it contains an equivalent amount of olive fruit extract instead of hydroxytyrosol, and the other components and amounts are the same as in Example 10.

[0063] Comparative Example 13

[0064] The composition in this example differs from Example 10 only in that it contains an equivalent amount of an extract of chamomile instead of sodium gualenate, and the other components and amounts are the same as in Example 10.

[0065] Comparative Example 14

[0066] The composition in this example differs from that of Example 10 only in that betaine is replaced by trehalose in an equivalent amount in this example, and the other components and amounts are the same as in Example 10.

[0067] Application Example 1

[0068] The compositions in Examples 1 to 10 and Comparative Examples 1 to 14 were respectively added to the serum base formula in Table 2 to produce Nos. 1 to 24 of serum, in which Nos. 1 to 10 of serum respectively used the compositions in Examples 1 to 10; and Nos. 11 to 24 of serum respectively used the compositions in Comparative Examples 1 to 14. The added amounts of the compositions in Examples 1 to 10 and Comparative Examples 1 to 14 are shown in Table 3, based on 100% of the total mass of the serum.

[0069] Application Example 2

[0070] The compositions in Examples 1 to 10 and Comparative Examples 1 to 14 were respectively added to the eye cream base formula in Table 2 to produce Nos. 1 to 24 of eye cream, in which Nos. 1 to 10 of eye cream respectively used the compositions in Examples 1 to 10; and Nos. 11 to 24 of eye cream respectively used the compositions in Comparative Examples 1 to 14. The added amounts of the compositions in Examples 1 to 10 and Comparative Examples 1 to 14 are shown in Table 3, based on 100% of the total mass of the eye cream.

[0071] Application Example 3

[0072] The compositions in Examples 1 to 10 and Comparative Examples 1 to 14 were respectively added to the pack liquid base formula in Table 2 to produce Nos. 1 to 24 of pack liquid, in which Nos. 1 to 10 of pack liquid respectively used the compositions in Examples 1 to 10; and Nos. 11 to 24 of pack liquid respectively used the compositions in Comparative Examples 1 to 14. The added amounts of the compositions in Examples 1 to 10 and Comparative Examples 1 to 14 are shown in Table 3, based on 100% of the total mass of the pack liquid.

[0073] Table 2 Base formula of serum, eye cream and pack liquid

[0074]

[0075]

[0076] Table 3 Added amounts of the compositions in Examples 1 to 10 and Comparative Examples 1 to 14

[0077]

[0078]

[0079] Product performance test

[0080] (1) Anti-inflammatory evaluation test

[0081] Select 90 sensitive skin volunteers, aged 20-50 years old, randomly divide the patients into 9 groups, 10 people in each group, after washing face in the morning and evening, respectively use the essence (the base formula of the essence is shown in Table 2) added with the compositions of Examples 7-10 and Comparative Examples 3, 6, 8, 10, 13, continuously use for 4 weeks, apply once in the morning and evening. Statistics of the use, the results are shown in Table 4.

[0082] Anti-inflammatory effect criteria:

[0083] Cure: the symptoms of skin redness, itching, pain completely disappear;

[0084] Effective: the symptoms of skin redness, itching, pain basically disappear;

[0085] Ineffective: the symptoms of skin redness, itching, pain are the same as before or aggravated.

[0086] Table 4 Anti-inflammatory repair effect evaluation results (number of people)

[0087]

[0088]

[0089] From the results of Table 4, it can be seen that the essence containing the compositions of Examples 7-10 of the application has good anti-inflammatory and repair effect, and it can be found that within a certain concentration range, the higher the content of guaiazulene sulfonate sodium, the better the anti-inflammatory repair effect. Through comparative experiments, it is found that removing guaiazulene sulfonate sodium from the composition, or replacing guaiazulene sulfonate sodium with other components, the effect is obviously reduced; removing guaiazulene sulfonate sodium and any one component in the composition, the effect almost disappears. Therefore, it can be inferred that guaiazulene sulfonate sodium is compounded with hydroxytyrosol, betaine and methylvaleronate lactone components, and through synergistic effect, the best anti-inflammatory effect is achieved.

[0090] (2) Skin wrinkle area test

[0091] Select 24 volunteers, use the eye cream added with the compositions of Examples 1-10 and Comparative Examples 1-14 on the left face, and use the blank control eye cream (the blank control eye cream is prepared according to the base formula of the eye cream in Table 2, without adding the compositions in Examples or Comparative Examples) on the right face, the subjects use it once a day, and use it for 4 weeks according to the product use method; analyze the use effect of the product, and use the skin wrinkle area reduction rate to represent, the larger the skin wrinkle area reduction rate value, the better the effect.

[0092] The area reduction rate of skin wrinkles = (total number of wrinkles in the designated area before the test - total number of wrinkles in the designated area after the test) / total number of wrinkles in the designated area before the test x 100%, and the test results calculated according to the formula are shown in Table 5.

[0093] The comparative graphs of the eye wrinkles before and after using the eye cream added with the composition of Example 10 are shown in FIG. 2. Figure 1 .

[0094] Table 5 Area reduction rate of skin wrinkles

[0095]

[0096]

[0097] As can be seen from Table 5, the area reduction rate of skin wrinkles around the eyes after 4 weeks of using the eye cream added with the composition of the examples is 7.8-18.2%, which is significantly higher than the area reduction rate of skin wrinkles (1.2-6.5%) after 4 weeks of using the eye cream added with the composition of the comparative examples. By comparing Examples 1-3, 3-5, 5-7, and 7-9, it can be found that within the range of the amount of addition defined in the present application, the content of a certain component of the composition is reduced, and the wrinkle-removing effect is also reduced. By comparing Comparative Examples 1-14, it can be found that if any one or more components of the composition is removed or the components of the composition are replaced with other components, the anti-wrinkle effect will be significantly reduced. The blank control group without adding the composition has almost no change. As can be seen from FIG. 1, the eye wrinkles are significantly reduced after 28 days of using the eye cream added with the composition of Example 10. Figure 1 As can be seen from Table 5, the area reduction rate of skin wrinkles around the eyes after 4 weeks of using the eye cream added with the composition of the examples is 7.8-18.2%, which is significantly higher than the area reduction rate of skin wrinkles (1.2-6.5%) after 4 weeks of using the eye cream added with the composition of the comparative examples. By comparing Examples 1-3, 3-5, 5-7, and 7-9, it can be found that within the range of the amount of addition defined in the present application, the content of a certain component of the composition is reduced, and the wrinkle-removing effect is also reduced. By comparing Comparative Examples 1-14, it can be found that if any one or more components of the composition is removed or the components of the composition are replaced with other components, the anti-wrinkle effect will be significantly reduced. The blank control group without adding the composition has almost no change. As can be seen from FIG. 1, the eye wrinkles are significantly reduced after 28 days of using the eye cream added with the composition of Example 10.

[0098] (3) Skin elasticity test

[0099] Select 75 volunteers, three people in a group, respectively use the mask liquid added with the mask liquid of examples 1-10, comparative examples 1-14, and ordinary fiber film cloth is used as face mask, 3 times a week, use a piece of mask 15-20 min each time; The blank control group mask liquid is prepared according to the basic formula of the mask liquid in table 2, without adding the composition in examples or comparative examples. Take the skin of the cheekbone and the corner of the eye as the test site. According to the group standard T / ZHCA 005-2019 'cosmetic influence on skin elasticity test method', the skin elasticity characterization value R before and after 28 days of use is determined respectively, the greater the R value change rate (i.e. V) indicates the better the skin elasticity improvement, the calculation formula of the characterization value R is: R=U1 / U2. In the formula, U1 is the rebound displacement of the skin after the negative pressure is eliminated (mm), U2 is the maximum stretching displacement of the skin when the negative pressure is loaded (mm). The change rate V=(R2-R1) / R1*100%; Wherein, R1 is the skin elasticity characterization value before use, R2 is the skin elasticity characterization value after use, and the test average value of the skin elasticity change rate of each group is shown in table 6.

[0100] Table 6 skin elasticity change rate

[0101]

[0102] From the above table 6, after using the mask liquid added with the composition of the examples of the application for 28 days, the skin elasticity change rate is 12.52-18.55%, which is significantly higher than that of the blank control group and the mask liquid added with the composition in the comparative examples (2.15-9.20%) after 28 days, further indicating that the components in the composition of the application have a synergistic effect, which can significantly increase the skin elasticity. In addition, by comparing the mask liquid added with examples 1-3, 3-5, 5-7, 7-9, it can be seen that within the addition amount range defined in the application, increasing the content of a component of the composition can further improve the skin elasticity change rate after using the mask liquid.

[0103] In summary, the methylvaleronolactone in the composition of the application is helpful to maintain the elasticity and moisture content of the skin; hydroxytyrosol is a natural polyphenol compound with strong antioxidant activity, which can also promote wound healing, eliminate free radicals, remove wrinkles and anti-aging, and has anti-inflammatory effect, which can reduce skin inflammation and swelling; guaiacol sulfonate sodium has excellent anti-inflammatory, anti-allergic and tissue repair effects; betaine has excellent moisturizing ability, which can make the face smoother after use, and the combination of it and other components can also be used to reduce skin allergy, which is an ideal soothing moisturizing agent. Through compatibility study, it is accidentally found that the combination of methylvaleronolactone, hydroxytyrosol, guaiacol sulfonate sodium and betaine has a synergistic effect, so as to realize excellent anti-inflammatory, wrinkle removal and anti-aging effect.

[0104] The preferred embodiments of the present application have been disclosed with specific reference to a preferred embodiment. A person with ordinary skill in the art understands that variations in, or replacements for, the preferred embodiments described herein can be made without departing from the spirit of the application. These equivalent variations or replacements are also encompassed within the scope of the claims defined below.

Claims

1. An anti-wrinkle composition, characterized in that: The anti-wrinkle composition comprises the following components in parts by weight: 0.01-0.5 parts of mevalonolactone, 0.001-0.05 parts of hydroxytyrosol, 0.001-0.05 parts of sodium guaiacylsulfonate and 0.1-5 parts of betaine.

2. The anti-wrinkle composition according to claim 1, characterized in that The anti-wrinkle composition comprises the following components in parts by weight: 0.01-0.1 part of mevalonolactone, 0.001-0.01 part of hydroxytyrosol, 0.001-0.01 part of sodium guaiacylsulfonate and 0.1-1 part of betaine.

3. The anti-wrinkle composition according to claim 1, characterized in that The anti-wrinkle composition comprises the following components in parts by weight: 0.1-0.5 parts of mevalonolactone, 0.01-0.05 parts of hydroxytyrosol, 0.01-0.05 parts of sodium guaiazulene sulfonate and 1-5 parts of betaine.

4. The method for preparing the anti-wrinkle composition according to any one of claims 1 to 3, characterized in that: The following steps are involved: The components are mixed to obtain an anti-wrinkle composition.

5. A skin preparation for external use, characterized in that The external skin preparation comprises the anti-wrinkle composition according to any one of claims 1 to 3.

6. The external skin preparation according to claim 5, wherein The content of the anti-wrinkle composition is 0.1-6 wt %.

7. A cosmetic, characterized in that: The cosmetic comprises the anti-wrinkle composition according to any one of claims 1 to 3.

8. The cosmetic according to claim 7, characterized in that The content of the anti-wrinkle composition is 0.1-6 wt %.

9. The cosmetic according to claim 7, characterized in that The cosmetics include any one of essence, lotion, cream or facial mask.

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