A white hair turning black hair essence and a preparation method thereof

Abstract

The application discloses a white hair to black hair essence and a preparation method thereof. The white hair to black hair essence is prepared from selected raw materials, such as water dropwort leaf / stem extract, oxyhematin, radix polygoni multiflori, panax notoginseng and the like, and has good effects on activating hair follicles, enhancing the activity of melanocytes in the hair follicles and promoting melanin production. Volunteer tests prove that with the growth of new black hair and the shedding of aging white hair, the black hair gradually increases and the white hair gradually decreases by long-term use of the white hair to black hair essence, and the sensory effect of 'white hair to black hair' is achieved.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of cosmetics, in particular a white hair to black hair essence and a preparation method thereof. BACKGROUND

[0002] Hair whitening is a complex process involving multiple factors. In most cases, hair naturally whitens with age, which is a normal physiological phenomenon. However, the current fast-paced life, increased work and mental stress, and lack of rest and sleep have caused an imbalance in the nutrients required by the hair, resulting in an increasing number of young people with white hair. White hair has become an important factor affecting people's image and physical and mental health, and has become a key research object in the field of hair care.

[0003] Hair contains a large amount of melanin, which is synthesized by hair follicle melanocytes. The melanin synthesis and metabolism process can be divided into the following stages: melanin synthesis in melanocytes; melanin granules are released from the cells in a cell secretion manner and transferred to the surrounding keratinocytes; and the process of melanin granules melting in keratinocytes. Any obstacle in the generation, transfer and melting of melanin granules can affect melanin metabolism, thereby causing changes in hair color.

[0004] Domestic and foreign research on white hair has mainly focused on its causes and pathogenesis, that is, most people believe that the decrease in tyrosinase activity is the main factor affecting the generation and deposition of hair pigments. The decrease in tyrosinase activity, the decrease in melanin synthesis, the decrease in pigment deposition, and the resulting hair whitening. At the same time, the interaction between melanin and keratinocytes is weakened, the migration of melanocytes from the outer root sheath reserve to the hair follicle papilla is reduced, the normal pigmentation function is destroyed, and white hair can also be caused. At the same time, the free radical theory is also the mechanism of hair whitening, which is related to genetics, trace elements, and vitamin abnormalities.

[0005] The present inventors have found that the combination of watercress leaf / stem extract, oxyhematin, radix polygoni multiflori, and panax notoginseng has good effects on activating hair follicles, enhancing the activity of melanocytes in hair follicles, and promoting melanin generation. With the growth of new black hair and the shedding of aging white hair, the use of the combination for a long time, the number of black hair gradually increases, and the number of white hair gradually decreases, thereby achieving the sensory effect of "white hair to black hair". SUMMARY

[0006] The present application relates to the field of cosmetics, in particular a white hair to black hair essence and a preparation method thereof. The present application relates to the field of cosmetics, in particular a white hair to black hair essence and a preparation method thereof.

[0007] The specific technical solutions of the present application are as follows:

[0008] The white hair turning black essence contains the following components in the following mass percentage: Nasturtium officinale leaf / stem extract 1-10%, oxyhematin 1-10%, Polygonum multiflorum root extract 1-10%, and Panax notoginseng root extract 1-10%.

[0009] In one embodiment, the white hair turning black essence contains the following components in the following mass percentage: Nasturtium officinale leaf / stem extract 7%, oxyhematin 5%, Polygonum multiflorum root extract 3%, and Panax notoginseng root extract 3%.

[0010] Preferably, the white hair turning black essence can further contain the following components in the following mass percentage: moisturizing agent 1-10% and emollient 0.1-5%. The moisturizing agent and the emollient are added to maintain the moisture on the scalp for a long time, maintain and protect the water and fat film on the scalp surface, and build a healthy environment for the scalp.

[0011] The moisturizing agent can be polyhydric alcohol such as glycerol, butylene glycol, polyethylene glycol, propylene glycol, hexylene glycol, xylitol, and sorbitol; pseudo-natural moisturizing factor such as amino acid, pyrrolidone carboxylic acid, sodium lactate, urea, and ceramide; sugar such as methyl gluceth, chitin derivative, trehalose, tremella polysaccharide, sodium hyaluronate, and sugar isomer; and panthenol and silk protein.

[0012] The emollient can be one or more than one of silicone oil, vegetable oil, animal oil, and synthetic oil.

[0013] Preferably, the white hair turning black essence can further contain the following components in the following mass percentage: heat-sensing agent 0.1-1%. The heat-sensing agent can produce a warm feeling, making people feel more comfortable. It can also stimulate vasodilation, accelerate blood circulation, promote body metabolism, and facilitate the absorption of other active ingredients.

[0014] The heat-sensing agent can be capsaicin, chili extract, xanthal base butyl ether, gingerol, and ginger extract.

[0015] Preferably, the white hair to black hair essence can further contain the following components in the following mass percentage: an anti-dandruff agent 0.1-1%. The anti-dandruff agent can inhibit and kill fungi and bacteria, soften the stratum corneum, promote the exfoliation of the stratum corneum, and the like, can remove dandruff, relieve the itching of the scalp, improve the secretion of sebum on the scalp, and promote the healthy growth of hair.

[0016] The anti-dandruff agent can be selenium disulfide, salicylic acid, pyrithione zinc, dipotassium glycyrrhizinate, climbazole, piroctone olamine, and the like synthetic / semi-synthetic materials, or can be plant extracts with dandruff-removing effects extracted, purified, and compounded from tea tree, radix scutellariae, radix coptidis, radix cnidii, radix meliae, radix stachyuri, radix taraxaci, radix agrimoniae, and / or radix sophorae flavescentis.

[0017] Preferably, the white hair to black hair essence can further contain the following components in the following mass percentage: an anti-allergic agent 0.1-1%. The anti-allergic agent has the functions and effects of inhibiting the release of histamine, relieving itching, resisting inflammation, improving the congestion of mucous membranes, and the like, can relieve the discomfort of the scalp, eliminate inflammation, and is conducive to the healthy growth of hair. The anti-allergic agent can be one or more than one mixture of farnesol, extract of spilanthes acmella, extract of chamomile, extract of oat kernel, and sodium gualenate.

[0018] The technical scheme provided by the embodiment of the present application has the following beneficial effects:

[0019] (1) According to the scheme of monarch, minister, assistant and messenger, the leaf / stem extract of Brassica rapa L. is selected as the monarch drug, the hemin and radix polygoni multiflori extract are selected as the minister drug, and the root extract of Panax notoginseng is selected as the assistant and messenger drug. The leaf / stem extract of Brassica rapa L. can activate hair follicles, improve white hair, and promote hair growth; the hemin can repair hair and promote the generation of melanin; the radix polygoni multiflori extract can enhance the activity of melanocytes in hair follicles and promote the generation of melanin in hair follicles; and the root extract of Panax notoginseng can relieve inflammation, resist environmental stress, and protect hair follicle cells. The four active substances have good effects on activating hair follicles, enhancing the activity of melanocytes in hair follicles, and promoting the generation of melanin.

[0020] (2) The white hair to black hair essence provided by the embodiment of the present application further contains a humectant, a fat-impregnating agent, a heat-sensing agent, an anti-dandruff agent, and an anti-allergic agent, which can effectively maintain and protect the water and fat film on the surface of the scalp, stimulate the dilation of blood vessels, promote the absorption of active ingredients, relieve the discomfort of the scalp, improve the secretion of sebum on the scalp, effectively build a healthy environment for the scalp, and promote the healthy growth of hair. The results of the trial by volunteers prove that the white hair to black hair essence provided by the embodiment of the present application can gradually increase the black hair and reduce the white hair of the volunteers if used for a long time, and the sensory effect of “white hair to black hair” is achieved. BRIEF DESCRIPTION OF DRAWINGS

[0021] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the drawings needed to be used in the embodiments or prior art description are briefly introduced as follows.

[0022] Figure 1 Figure 3 shows the comparison of the photos of the volunteers before and after using the white hair turning black essence. DETAILED DESCRIPTION

[0023] The present application is described in more detail by specific examples, and other advantages and effects of the present application can be easily understood by those skilled in the art from the disclosure of the present application. The present application can also be implemented or applied by different specific embodiments, and various modifications or changes can be made to the details in the present specification based on different views and applications without departing from the spirit of the present application.

[0024] Before further describing the specific embodiments of the present application, it should be understood that the scope of protection of the present application is not limited to the following specific embodiments; it should also be understood that the terms used in the embodiments of the present application are for the purpose of describing the specific embodiments, and are not intended to limit the scope of protection of the present application.

[0025] When the embodiments give numerical ranges, it should be understood that, unless otherwise specified by the present application, both endpoints of each numerical range and any number between the two endpoints can be selected. Unless otherwise defined, all technical and scientific terms used herein have the same meaning as generally understood by those skilled in the art to which the present application belongs.

[0026] It is worth noting that the raw materials used in the present application are all ordinary commercially available products, and therefore their sources are not specifically limited.

[0027] The NASTURTIUM OFFICINALE leaf / stem extract described in the present application is extracted from the aboveground parts of the NASTURTIUM OFFICINALE, a perennial aquatic herbaceous plant of the Brassicaceae family. NASTURTIUM OFFICINALE has a crisp and tender taste, and is sweet and delicious, and can be cooked into various dishes. Its whole grass can be used as medicine, and has the effects of clearing the lungs, cooling the blood, diuresis and detoxification. Modern research has found that NASTURTIUM OFFICINALE leaf / stem extract can activate hair follicles, improve white hair and promote hair growth. Suitable NASTURTIUM OFFICINALE leaf / stem extract can include BURGEON-UP (from ICHIMARU PHARCO Co., Ltd, Japan).

[0028] The hemin described in the present application is a raw material for Japanese quasi drugs, has the effect of promoting tyrosinase activity, and in addition, has an iron atom in the center of the porphyrin structure, can be combined with histidine and cysteine in hair keratin, has strong adhesion to hair, and plays a repair effect on hair. Suitable hemin can include GROWTH PHYLLIN P (PF) (from Japan Ikemaru Co., Ltd.).

[0029] The polygonum multiflorum root extract described in the present application is extracted from the roots of polygonum multiflorum in the polygonaceae family. Polygonum multiflorum has the effects of tonifying essence and blood (processed), detoxifying, intercepting malaria, and moistening the intestines and relieving constipation (raw). It is used to treat essence and blood deficiency, dizziness and blurred vision, premature graying of hair, soreness of the waist and knees, chronic malaria, carbuncle, scrofula, and dry constipation. The source of the polygonum multiflorum root extract can be a commonly available product. Suitable polygonum multiflorum root extract can include Shou Wu Pe (from Guilin Lain Biological Technology Co., Ltd.).

[0030] The panax notoginseng root extract described in the present application is extracted from the roots of panax notoginseng, a perennial herb in the araliaceae family. Panax notoginseng is warm in nature, sweet and slightly bitter in taste, and belongs to the liver and stomach meridians. It has the effects of tonifying blood and resisting inflammation, promoting blood circulation and removing blood stasis, and can resist oxidation and aging. The source of the panax notoginseng root extract can be a commonly available product. Suitable panax notoginseng root extract can include Sanqi Extract (from Kunshan Jialong Biological Technology Co., Ltd.).

[0031] Test 1: Detection of intracellular melanin content and tyrosinase activity

[0032] In order to verify the ability of the white hair to black hair essence provided by the present application to enhance the activity of melanocytes in hair follicles and promote melanin production, this test is specially designed.

[0033] Mouse melanoma cells B16F10 are low in cost, highly repeatable, and short in experimental period, and are a commonly used model for melanin research. The test is carried out according to the method recorded in the paper "Huang Jie, et al. Synergistic whitening, antioxidant and anti-inflammatory activity of glabridin-resveratrol-reynoutrin combination [J]. Perfume Flavor and Cosmetics, 2024, (03): 99-107+233." to measure the melanin content and tyrosinase activity.

[0034] Specific steps: B16F10 was inoculated in 6-well plates (1×10 5The cells were collected and the precipitate was collected per well and 0.4 mL of 1 mol / L NaOH solution (containing 10% DMSO by volume) was added, incubated at 80°C for 30 min, and the absorbance was detected at 490 nm.

[0035] (1) Melanin content: The cell precipitate was collected per well and 0.4 mL of 1 mol / L NaOH solution (containing 10% DMSO by volume) was added, incubated at 80°C for 30 min, and the absorbance was detected at 490 nm.

[0036] (2) Tyrosinase activity: The cell precipitate was collected per well and 0.5 mL of 0.5% sodium deoxycholate solution was added, lysed on ice for 15 min, then warmed at 37°C and 0.25 mL of 0.3% dopa solution was added, reacted at 37°C for 30 min, and the absorbance was detected at 475 nm.

[0037] Table 1 Effect of test substances on melanin content and tyrosinase activity

[0038]

[0039] As can be seen from Table 1, the melanin inhibition rate and tyrosinase inhibition rate of each test group are negative, indicating that they all show the effect of active tyrosinase and promote melanin production. Among the five test groups, test group 1 added NASTURTIUM OFFICINALE leaf / stem extract, hemin, POLYGONUM MULTIFLORUM root extract, and P. NOTOGINSENG root extract showed better results.

[0040] Test 2 Chicken embryo chorioallantoic membrane test

[0041] The chorioallantoic membrane (CAM) is a respiratory membrane surrounding the chicken embryo. Since the chicken embryo chorioallantoic membrane surface is rich in blood vessels, it can be regarded as a complete organism. This test takes advantage of the characteristics of the chorioallantoic membrane system of the incubated chicken embryo, which is complete, clear and transparent. A certain amount of test substance is directly contacted with the chorioallantoic membrane of the chicken embryo, and after a specified time of action, the changes in the toxicity effect indicators (such as bleeding, clotting and vascular fusion) of the chorioallantoic membrane are observed, scored and the mathematical average value is calculated to evaluate the irritability of the test substance. The test was performed according to the method described in the literature “Wang Xianhao, et al. Study on the soothing and repairing effects of two plant extract compositions on skin [J]. Shandong Chemical Industry, 2024, 53(05): 43-45+50.”.

[0042] The test procedure is as follows: SPF chicken eggs are placed in a constant temperature incubator at 37.5±0.5°C and a relative humidity of 55-70% to be incubated until 9 days old. The negative control is a 0.9% sodium chloride solution prepared from deionized water, which is sterilized and used. The positive control is a sodium lauryl sulfate (SLS) solution prepared from physiological saline, and the final test concentration is 1%. 0.3 mL of physiological saline, 1% SLS solution, and the test sample (Nasturtium officinale leaf / stem extract, oxyhematin, Polygonum multiflorum root extract, Panax notoginseng root extract mixed at a ratio of 1:1:1:1) are added dropwise to the CAM surface, the CAM reaction is observed, and the time at which the blood vessel bleeding, coagulation, and blood vessel dissolution effects appear within 300 s are recorded, and the irritation score value (IS) and classification are calculated. There are 6 replicates in each treatment group.

[0043] IS < 1 is non-irritating;

[0044] 1 ≤ IS < 5 is mild irritation;

[0045] 5 ≤ IS < 9 is moderate irritation;

[0046] IS ≥ 9 is severe irritation.

[0047] IS = (301-bleeding time)*5 / 300 + (301-bleeding dissolution time)*5 / 300 + (301-coagulation time)*9 / 300.

[0048] The test results show that after treating the CAM with the SLS solution, obvious bleeding occurs within 300 s, and the IS value is 12.38, which belongs to severe irritation; after treating the CAM with the test sample, no bleeding occurs within 300 s, and the IS value is 0, which shows no irritation. Therefore, the active ingredients of the white hair blackening essence are mild and show no irritation.

[0049] Example 1

[0050] A white hair blackening essence contains the following components in the following mass percentages: Nasturtium officinale leaf / stem extract 1%, oxyhematin 10%, Polygonum multiflorum root extract 1%, Panax notoginseng root extract 10%, 1,3-propanediol 1%, bis-aminopropyl dimethicone 5%, ginger extract 0.1%, piroctone olamine 1%, Portulaca oleracea extract 0.1%, 1,2-pentanediol 1%, p-hydroxyacetophenone 0.4%, and water in the balance.

[0051] Example 2

[0052] A white hair to black hair essence containing the following components in mass percentage: Nasturtium officinale leaf / stem extract 10%, hemin 1%, Polygonum multiflorum root extract 10%, Panax notoginseng root extract 1%, 1,3-propanediol 10%, bis-aminopropyl dimethicone 0.1%, ginger extract 1%, piroctone olamine 0.1%, Portulaca oleracea extract 1%, 1,2-pentanediol 0.5%, p-hydroxyacetophenone 0.6%, and water in balance.

[0053] Example 3

[0054] A white hair to black hair essence containing the following components in mass percentage: Nasturtium officinale leaf / stem extract 7%, hemin 5%, Polygonum multiflorum root extract 3%, Panax notoginseng root extract 3%, 1,3-propanediol 5%, bis-aminopropyl dimethicone 2.5%, ginger extract 0.5%, piroctone olamine 0.5%, Portulaca oleracea extract 0.5%, 1,2-pentanediol 0.8%, p-hydroxyacetophenone 0.5%, and water in balance.

[0055] Test 3 volunteer test

[0056] Volunteers with white hair problems were recruited, with no gender limit, aged 30-50 years old. They were randomly divided into 2 groups, 30 in each group. The white hair to black hair essence prepared in Example 3 was tested in Group 1, and a certain brand of hair blackening essence on the market was tested in Group 2.

[0057] Method of use: The essence was filled into a dropper bottle, and after the hair was cleaned, washed, and dried, the product was added to the white hair with a dropper, with an amount of about 5-15 mL, and evenly combed with a comb. It was used once in the morning and once in the evening, for a continuous use of 3 months. The volunteers were visited at the 1st month, 2nd month, and 3rd month, and the self-evaluation results of the volunteers were collected to calculate the average score of each group.

[0058] Volunteer self-evaluation standard for white hair improvement: 0 points for no obvious effect of white hair to black hair; 3 points for moderate improvement, with the feeling that the white hair has become less; and 5 points for very good improvement, with the white hair obviously becoming less and the black hair becoming more.

[0059] Table 2 Self-evaluation results of volunteers for white hair improvement

[0060] January February March Example 3 2.15±0.56 3.07±1.11 4.68±0.87 Commercial product 1.02±0.12 1.87±1.02 3.12±1.23

[0061] As shown in Table 2, the white hair turning black essence prepared by the embodiment of the present application has obvious white hair turning black effect, which is better than that of the commercially available product, and the effect is better and better with the extension of the use time, and excellent technical effect is achieved.

[0062] It should be noted that the above content is only used to illustrate the technical solutions of the present application, and is not a limitation on the protection scope of the present application. Any simple modification or equivalent replacement within the technical range disclosed by the present application does not deviate from the essence and scope of the technical solutions of the present application.

Claims

1. A white hair to black hair essence, characterized by, The composition comprises the following components in the following mass percentages: Nasturtium officinale leaf / stem extract 1-10%, hemin 1-10%, Polygonum multiflorum root extract 1-10%, Panax notoginseng root extract 1-10%, humectant 1-10%, and emollient 0.1-5%.

2. The white hair to black hair serum according to claim 1, characterized in that, The mass percentages of the components are as follows: Nasturtium officinale leaf / stem extract 7%, hemin 5%, Polygonum multiflorum root extract 3%, and Panax notoginseng root extract 3%.

3. The white hair to black hair serum according to claim 1, characterized in that, The humectant is one or more than one mixture of polyhydric alcohol, pseudo-natural moisturizing factor, sugar, and multifunctional active ingredient with moisturizing and nutritional properties.

4. The white hair to black hair serum as claimed in claim 1, wherein, The emollient is one or more than one mixture of silicone oil, vegetable oil, animal oil, and synthetic oil.

5. The white hair to black hair serum according to claim 1 or 2, characterized by, The composition further comprises the following component in the following mass percentage: hot-sensing agent 0.1-1%.

6. The white hair to black hair serum as claimed in claim 5, characterized in that, The hot-sensing agent is one or more than one mixture of capsaicin, chili extract, xanthal base butyl ether, gingerol, and ginger extract.

7. The white hair to black hair serum according to claim 1 or 2, characterized by, The composition further comprises the following component in the following mass percentage: anti-dandruff agent 0.1-1%.

8. The white hair to black hair serum as claimed in claim 7, characterized in that, The anti-dandruff agent is one or more than one mixture of selenium disulfide, salicylic acid, zinc pyrithione, dipotassium glycyrrhizinate, climbazole, piroctone olamine, tea tree, Scutellaria baicalensis, Coptis chinensis, Cnidium monnieri, Sanguisorba officinalis, Stachys asiatica, Taraxacum officinale, Agrimonia pilosa, and Sophora flavescens.

9. The white hair to black hair serum according to claim 1 or 2, characterized by, The composition further comprises the following component in the following mass percentage: anti-allergic agent 0.1-1%. The anti-allergic agent is one or more than one mixture of bisabolol, marigold extract, chamomile extract, oat kernel extract, and guaiazolone sodium sulfonate.

Images