Moisturizing and wrinkle-fading composition containing recombinant collagen and application of moisturizing and wrinkle-fading composition

Abstract

The invention relates to a moisturizing and wrinkle fading composition containing recombinant collagen and application of the moisturizing and wrinkle fading composition. The moisturizing and wrinkle fading composition containing the recombinant collagen comprises dipalmitoyl hydroxyproline, butyrospermum parkii fruit fat unsaponifiable matter, sodium hyaluronate, palmitoyl tripeptide-1 and the recombinant collagen. The invention creatively finds that the composition consisting of the dipalmitoyl hydroxyproline, the butyrospermum parkii fruit fat unsaponifiable matter, the sodium hyaluronate, the palmitoyl tripeptide-1 and the recombinant collagen can deeply nourish the skin, especially the lip skin, effectively lock moisture and relieve dryness, and also can remarkably lighten cheilogramma and repair damaged skin, so that the composition has the effects of nourishing the skin, especially the lip skin. And the five components have a certain synergistic effect on the effects, so that the lip care product is suitable for lip care products, and is mild, non-irritant and good in stability.

Description

Technical Field

[0001] The invention belongs to the technical field of skin care products and relates to a moisturizing and wrinkle-lightening composition containing recombinant collagen and application thereof. Background Art

[0002] In recent years, with the improvement of living standards and the enhancement of beauty awareness, consumers' demands for skin care products have gradually diversified. As one of the most delicate and vulnerable parts of the face, the lips have different requirements for skin care products than other parts. The thickness of the lip skin is only one-third of the facial skin, and it does not have sebaceous glands, which makes the lips less moisturizing and easily affected by environmental factors such as wind, sun, dry air, etc., resulting in chapped skin, peeling and fine lines. Therefore, there is an urgent need for lip care products with moisturizing and wrinkle-reducing effects in the market.

[0003] At present, there are many kinds of lip care products on the market, but most of them only stay at the basic moisturizing level, and the effect on fading lip lines and repairing damaged lip skin is limited. Although traditional moisturizing ingredients such as glycerin and vaseline can relieve dry lips to a certain extent, long-term use can easily form dependence, and the effect on improving lip lines is not obvious. At the same time, some products containing irritating ingredients not only cannot effectively improve lip problems, but may also increase the burden on lip skin and cause allergies or inflammation. In order to develop a composition that can deeply moisturize, promote the regeneration and repair of lip skin, and effectively repair lip fine lines in response to the special needs of lip skin, it is necessary to comprehensively consider the biocompatibility, moisturizing effect, penetration ability and adaptability to lip skin of its ingredients.

[0004] Based on this, it is necessary to develop a composition with moisturizing and wrinkle-lightening effects to fill the gap in lip care products on the market and provide consumers with a safe and efficient lip care solution. Summary of the invention

[0005] In view of the deficiencies of the prior art, the object of the present invention is to provide a moisturizing and wrinkle-reducing composition containing recombinant collagen and its application.

[0006] In order to achieve the purpose of the invention, the present invention adopts the following technical solutions:

[0007] In a first aspect, the present invention provides a moisturizing and wrinkle-reducing composition containing recombinant collagen, wherein the moisturizing and wrinkle-reducing composition containing recombinant collagen comprises dipalmitoyl hydroxyproline, avocado oil unsaponifiable matter, sodium hyaluronate, palmitoyl tripeptide-1 and recombinant collagen.

[0008] Among them, dipalmitoyl hydroxyproline can stimulate the tightening of collagen fibers, protect elastic fibers against enzyme degradation, capture free radicals, protect skin cells (such as fibroblasts), repair skin anti-aging defense (increase TIMPs), inhibit the activity of MMPs, protect collagen and DEJ structure, and has a stronger effect than vitamin C in stimulating collagen fiber contraction and inhibiting elastase activity.

[0009] Avocado tree (BUTYROSPERMUM PARKII) fruit fat unsaponifiable matter is extracted from avocado fruit. It has good moisturizing effect, can help skin and hair lock moisture for a long time, deeply nourish skin and hair, and improve their dryness and roughness problems.

[0010] Sodium hyaluronate can improve the physiological conditions of the skin, provide a superior external environment for the synthesis of dermal collagen and elastic fibers, strengthen the supply of nutrients, and play a role in skin care and beauty. Studies have shown that hyaluronic acid can prevent the production of some enzymes in cells, reduce the formation of free radicals, and play an important role in preventing free radicals from destroying cell structures, producing lipid peroxidation, and causing body aging. Low molecular weight hyaluronic acid has anti-inflammatory effects, inhibits the production of pathogens, and keeps the skin smooth; it provides a suitable place for cell proliferation and differentiation, and directly promotes cell growth, differentiation, reconstruction and repair.

[0011] Palmitoyl tripeptide-1 is a matrikine signal peptide that acts on the dermis and can promote the synthesis of extracellular matrix such as collagen and glycosaminoglycans, strengthen the dermis, make the skin thicker and firmer, relieve wrinkles, and have a stronger ability to resist ultraviolet radiation.

[0012] Recombinant collagen contains a large amount of hydrophilic genes, has good film-forming properties, and maintains moisture in the stratum corneum of the skin. At the same time, its tropism can guide epithelial cells to quickly enter the damaged area, effectively increase the speed of skin regeneration, shorten the wound healing time, and thus restore the skin barrier function.

[0013] The present invention creatively discovers that the composition of five ingredients, namely, dipalmitoyl hydroxyproline, unsaponifiable matter of avocado butter, sodium hyaluronate, palmitoyl tripeptide-1 and recombinant collagen, can not only deeply nourish the skin, especially the lip skin, effectively lock in moisture, and relieve dryness, but also significantly reduce lip lines, repair damaged skin, and restore the lips to tenderness and smoothness. The five ingredients have a certain synergistic effect in the above-mentioned effects, and are suitable for use in lip care products, are mild and non-irritating, and have good stability.

[0014] Preferably, the nucleotide sequence encoding the recombinant collagen is the sequence shown in SED ID NO:1.

[0015] Compared with recombinant collagen from other sources, the present invention also found that the recombinant collagen from the above specific source combined with dipalmitoyl hydroxyproline, avocado unsaponifiable matter, sodium hyaluronate, and palmitoyl tripeptide-1 has a better effect in moisturizing and reducing wrinkles.

[0016] SED ID NO:1:

[0017] .

[0018] Preferably, the moisturizing and wrinkle-reducing composition containing recombinant collagen comprises, by weight, 1-5 parts of dipalmitoyl hydroxyproline, 0.1-3 parts of avocado unsaponifiable matter, 1-5 parts of sodium hyaluronate, 1-5 parts of palmitoyl tripeptide-1 and 0.01-1 part of recombinant collagen.

[0019] Furthermore, when the above five components are combined in a specific mass ratio, they have more significant effects in moisturizing and reducing wrinkles.

[0020] The above weight proportions are calculated based on the actual effective ingredients dipalmitoyl hydroxyproline, avocado unsaponifiable matter, sodium hyaluronate, palmitoyl tripeptide-1 and recombinant collagen contained in the commercially available raw materials. The commercially available raw materials may also selectively contain any one or a combination of at least two of solvents, fillers, diluents, stabilizers, pH adjusters, antibacterial agents, antioxidants or impurities within the permitted range.

[0021] The weight proportions of the dipalmitoyl hydroxyproline can be 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts, etc.

[0022] The weight proportions of the unsaponifiable matter of avocado fat can be 0.1 parts, 0.2 parts, 0.5 parts, 0.7 parts, 0.9 parts, 1 parts, 1.5 parts, 2 parts, 2.5 parts, 3 parts, etc.

[0023] The weight proportions of the sodium hyaluronate can be 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts, etc.

[0024] The weight proportions of the palmitoyl tripeptide-1 can be 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts, etc.

[0025] The weight proportions of the human recombinant collagen can be 0.01 parts, 0.03 parts, 0.05 parts, 0.07 parts, 0.09 parts, 0.1 parts, 0.3 parts, 0.5 parts, 0.7 parts, 0.8 parts, 0.9 parts, 1 part, etc.

[0026] Other specific point values ​​within the above numerical ranges can be selected and will not be described in detail here.

[0027] In a second aspect, the present invention provides use of the moisturizing and wrinkle-reducing composition containing recombinant collagen according to the first aspect in the preparation of cosmetics.

[0028] Preferably, the cosmetic is applied to the eyes, face and / or lips.

[0029] Preferably, the mass percentage of the moisturizing and wrinkle-reducing composition containing recombinant collagen in cosmetics is 3-10%, for example 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, etc. Other specific point values ​​within this numerical range can be selected and will not be described here one by one.

[0030] Thirdly, the present invention provides a moisturizing and wrinkle-lightening cream containing recombinant collagen. The raw materials for preparing the cream include, in terms of mass percentage: 3-10% of the moisturizing and wrinkle-lightening composition containing recombinant collagen described in the first aspect, 5-20% of a moisturizer, 0.5-2% of an antioxidant, 0.5-2% of a skin feel regulator, 1-6% of an emulsifier, 15-50% of a moisturizer, 0.1-4% of a skin conditioner and 4-80% of water.

[0031] The mass percentage of the moisturizing and wrinkle-reducing composition containing recombinant collagen can be 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, etc.

[0032] The mass percentage of the moisturizing agent can be 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20% and the like.

[0033] The weight percentage of the antioxidant can be 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1%, 1.2%, 1.4%, 1.6%, 1.8%, 2%, etc.

[0034] The mass percentage of the skin feel regulator can be 0.5%, 0.6%, 0.7%, 0.8%, 0.9%, 1%, 1.2%, 1.4%, 1.6%, 1.8%, 2%, etc.

[0035] The mass percentage of the emulsifier can be 1%, 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, 4.5%, 5%, 5.5%, 6%, etc.

[0036] The mass percentage of the emollient can be 15%, 17%, 20%, 25%, 30%, 35%, 40%, 45%, 50%, etc.

[0037] The mass percentage of the skin conditioner can be 0.1%, 0.5%, 1%, 1.5%, 2%, 2.5%, 3%, 3.5%, 4%, etc.

[0038] The mass percentage of the skin conditioner can be 4%, 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, etc.

[0039] Other specific point values ​​within the above numerical ranges can be selected and will not be described in detail here.

[0040] Preferably, the skin conditioning agent comprises any one of allantoin, β-glucan, rice fermentation product filtrate or glyceryl glucoside, or a combination of at least two thereof.

[0041] Preferably, the skin conditioning agent is a combination of allantoin, β-glucan, rice fermentation product filtrate and glyceryl glucoside.

[0042] Preferably, the skin conditioning agent comprises, by weight, 0.1-1 part of allantoin, 0.1-1 part of β-glucan, 0.1-1 part of rice fermentation product filtrate and 0.1-1 part of glycerol glucoside.

[0043] Among them, 0.1-1 parts can be selected as 0.1 parts, 0.2 parts, 0.3 parts, 0.4 parts, 0.5 parts, 0.6 parts, 0.7 parts, 0.8 parts, 0.9 parts, 1 parts, etc. Other specific point values ​​within this numerical range can be selected, and they will not be listed here one by one.

[0044] The combination of skin conditioning agents allantoin, β-glucan, rice fermentation product filtrate and glycerol glucoside used in the moisturizing and wrinkle-reducing cream of the present invention can achieve a synergistic effect with the moisturizing and wrinkle-reducing composition containing recombinant collagen described in the first aspect, which is beneficial to further improve the moisturizing and wrinkle-reducing effect of the product.

[0045] Preferably, the moisturizing agent includes any one of glycerin, panthenol, fructose, butylene glycol or 1,2-hexanediol, or a combination of at least two thereof.

[0046] Preferably, the antioxidant comprises any one of tocopherol acetate, pentaerythritol tetrakis (di-tert-butyl hydroxyhydrocinnamate) or p-hydroxyacetophenone, or a combination of at least two thereof.

[0047] Preferably, the skin feel regulator includes any one or a combination of at least two of sodium acrylate / sodium acryloyldimethyl taurate copolymer, isohexadecane, polysorbate-80 or sorbitan isostearate.

[0048] Preferably, the emulsifier includes any one of cetyl alcohol, glyceryl stearate, PEG-75 stearate, ceteth-20, steareth-20 or steareth-21, or a combination of at least two thereof.

[0049] Preferably, the emollient comprises any one of C10-18 fatty acid triglycerides, avocado tree (BUTYROSPERMUMPARKII) fruit butter, petrolatum, squalane, cocoa seed butter, white beeswax, cetearyl alcohol, batyl alcohol, phytosteryl canola oil ester or polydimethylsiloxane, or a combination of at least two thereof.

[0050] In a fourth aspect, the present invention provides a method for preparing the cream having moisturizing and wrinkle-reducing effects according to the third aspect, the preparation method comprising:

[0051] (1) mixing water and a moisturizer, heating and keeping the temperature, to obtain a phase A; mixing an emulsifier, a moisturizer and an antioxidant, heating and keeping the temperature, to obtain a phase B;

[0052] (2) Mixing phase A and phase B, homogenizing, adding a skin feel regulator, homogenizing, cooling, adding a skin conditioner and a moisturizing and wrinkle-reducing composition containing recombinant collagen, and mixing to obtain the cream having moisturizing and wrinkle-reducing effects.

[0053] Preferably, the temperatures for heating and heat preservation are independently selected from 75-85°C, for example, 75°C, 76°C, 77°C, 78°C, 79°C, 80°C, 81°C, 82°C, 83°C, 84°C, 85°C, etc. Any other point value within this numerical range can be selected, and they will not be described here one by one.

[0054] Preferably, the cooling temperature is 40-50°C, for example, 40°C, 41°C, 42°C, 43°C, 44°C, 45°C, 46°C, 47°C, 48°C, 49°C, 50°C, etc. Any other point value within this numerical range can be selected, which will not be described here one by one.

[0055] Preferably, the homogenization speed is independently 1000-3000r / min, for example, 1000r / min, 1200r / min, 1400r / min, 1600r / min, 1800r / min, 2000r / min, 2200r / min, 2400r / min, 2600r / min, 2800r / min, 3000r / min, etc. Any other point value within the numerical range can be selected, which will not be described here one by one.

[0056] Compared with the prior art, the present invention has the following beneficial effects:

[0057] The present invention creatively discovers that the composition of five ingredients, namely, dipalmitoyl hydroxyproline, unsaponifiable matter of avocado butter, sodium hyaluronate, palmitoyl tripeptide-1 and recombinant collagen, can not only deeply nourish the skin, especially the lip skin, effectively lock in moisture, and relieve dryness, but also significantly reduce lip lines, repair damaged skin, and restore the lips to tenderness and smoothness. The five ingredients have a certain synergistic effect in the above-mentioned effects, and are suitable for use in lip care products, are mild and non-irritating, and have good stability. DETAILED DESCRIPTION

[0058] In order to further explain the technical means and effects adopted by the present invention, the technical solution of the present invention is further described below in conjunction with the preferred embodiments of the present invention, but the present invention is not limited to the scope of the embodiments.

[0059] In the following examples, unless otherwise specified, the reagents and consumables used were purchased from conventional reagent manufacturers in the field; unless otherwise specified, the experimental methods and technical means used were conventional methods and means in the field.

[0060] The weight percentages of dipalmitoyl hydroxyproline, avocado unsaponifiable matter, sodium hyaluronate, palmitoyl tripeptide-1 and recombinant collagen mentioned below are all calculated based on the actual content of effective ingredients contained in the commercially available raw materials. The commercially available raw materials may also selectively contain any one or a combination of at least two of solvents, fillers, diluents, stabilizers, pH adjusters, antibacterial agents, antioxidants or impurities within the permitted range.

[0061] The unsaponifiable matter of avocado tree fat mentioned in the following content comes from the product model NG SHEA UNSAPONIFIABLE purchased from SEDERMA, SAS;

[0062] Sodium hyaluronate was obtained from a product called PrimalHyal Gold purchased from Givaudan Fragrances & Fragrances (Shanghai) Co., Ltd.

[0063] PEG-75 stearate was obtained from GATTEFOSSE SAS under the model EMULIUM DELTA MB;

[0064] C10-18 fatty acid triglycerides were obtained from STEARINERIE DUBOIS FILS SA under the model DUBCIRE H1;

[0065] Petrolatum was obtained from a product of model MERKUR 500 purchased from Sasol (China) Chemical Co., Ltd.;

[0066] White beeswax was obtained from Wachs-u.Ceresin-Fabriken TH.C.TROMM GmbH under the model number Beeswax Bleached 3044PH;

[0067] Sodium acrylate / sodium acryloyldimethyl taurate copolymer was obtained from SEPPIC SA under the model SIMULGEL EG;

[0068] Polysorbate-80 was obtained from SEPPIC SA under the model SIMULGEL EG;

[0069] β-glucan was obtained from a product called Xishiyin SPG β-glucan purchased from Best Pharmaceutical (Guangzhou) Co., Ltd.;

[0070] The rice fermentation product filtrate was derived from a product called CelCaper sake lees extract purchased from Best Pharmaceutical (Guangzhou) Co., Ltd.;

[0071] Glycerol glucoside was derived from a product of model NaturalSuperM@2-αGG-40 purchased from Best Pharmaceuticals (Guangzhou) Co., Ltd.

[0072] The recombinant collagen involved in the following content is the product prepared in the preparation example. The pET-28a(+) vector in the preparation example was purchased from MacLean; the BL21(DE3) chemically competent cells were purchased from Merck with product number CMC0014-4X40UL.

[0073] Preparation Example

[0074] This preparation example provides a recombinant collagen freeze-dried powder, and the preparation method thereof is carried out with reference to the method disclosed in Preparation Example 1 in ZL 202310123476.3, as follows:

[0075] (1) Construction of recombinant plasmid: According to the nucleotide sequence shown in SEQ ID NO: 1, the pET-28a(+) vector suitable for the expression vector Escherichia coli host was selected and sent to a sequencing company for synthesis to obtain the recombinant collagen expression vector pET-28a-1;

[0076] (2) Construction of recombinant fermentation strain: The pET-28a-1 expression vector was transformed into competent Escherichia coli (BL21 (DE3)) cells by chemical transformation, and the positive clones in the transformed plate colonies were screened by agarose gel electrophoresis. The screened positive clones were transferred to 2 mL LB medium and cultured at 37°C, 200 rpm for 12 h. The cultured bacterial solution was diluted 1-fold with 50% glycerol by volume, dispensed into cryopreservation tubes, and frozen at -80°C to obtain a recombinant collagen fermentation strain;

[0077] (3) Fermentation culture of recombinant fermentation strains: 10 μL of recombinant collagen fermentation broth was spread on a solid LB plate, cultured in a 37°C incubator for 12 h, and then a single clone was selected and inoculated into 50 mL of LB liquid culture medium containing 50 μg / mL kanamycin. The culture was placed in a shaker at 37°C, 200 rpm for 14 h, and then inoculated into 200 mL of LB liquid culture medium containing 50 μg / mL kanamycin at a ratio of 1:100. The culture was cultured at 37°C until the bacterial OD = 0.6, and then IPTG was added to a final concentration of 1 mM. The culture was continued for 4 h, and then centrifuged at 4°C, 4000 rpm for 15 min to collect the bacteria.

[0078] (4) Purification of recombinant collagen: The cells were ultrasonically disrupted and centrifuged. The precipitate was washed with TritonX-100 washing solution and centrifuged again to remove the supernatant. 6M guanidine hydrochloride was added to the precipitate and the pH was adjusted to 8. After the precipitate was fully dissolved, the solution was passed through a nickel affinity chromatography resin and eluted in sequence using 20mM, 50mM, 100mM, 200mM, and 500mM concentration gradient imidazole solutions. The eluate was concentrated using a protein concentration column, and the concentrate was collected and dialyzed overnight (dialysis bag, RC membrane, 14KD, flat width 44mm (Shenggong F600112)). The protein concentration was then detected using a BCA kit, and 0.5mL of a 5% volume concentration mannitol aqueous solution was added to every 5mg of protein and mixed with the mannitol aqueous solution. The mixture was then pre-frozen at -40°C for 10min using a freeze dryer and finally freeze-dried at -35°C to obtain a recombinant collagen freeze-dried powder.

[0079] Example 1

[0080] This embodiment provides a moisturizing and wrinkle-reducing composition containing recombinant collagen, which includes the following effective ingredients by weight: 1.5 parts of dipalmitoyl hydroxyproline, 0.75 parts of unsaponifiable matter of avocado oil, 1.5 parts of sodium hyaluronate, 1.5 parts of palmitoyl tripeptide-1, and 0.1 parts of recombinant collagen. The preparation method is to mix the commercially available raw materials evenly.

[0081] Example 2

[0082] This embodiment provides a moisturizing and wrinkle-reducing composition containing recombinant collagen, which includes the following effective ingredients by weight: 1 part of dipalmitoyl hydroxyproline, 1 part of avocado unsaponifiable matter, 1 part of sodium hyaluronate, 2 parts of palmitoyl tripeptide-1, and 0.05 parts of recombinant collagen. The preparation method is to evenly mix the commercially available raw materials.

[0083] Example 3

[0084] This embodiment provides a moisturizing and wrinkle-reducing composition containing recombinant collagen, which includes the following effective ingredients by weight: 2 parts of dipalmitoyl hydroxyproline, 0.5 parts of unsaponifiable matter of avocado oil, 2 parts of sodium hyaluronate, 1 part of palmitoyl tripeptide-1, and 0.2 parts of recombinant collagen. The preparation method is to mix the commercially available raw materials evenly.

[0085] Example 4

[0086] This embodiment provides a moisturizing and wrinkle-reducing composition containing recombinant collagen, which differs from Example 1 only in that the recombinant collagen is replaced with commercially available recombinant collagen (purchased from Jinbo Biotechnology, with the trade name 164.88° Collagen (H0101)), and other conditions remain unchanged.

[0087] Comparative Example 1

[0088] This comparative example provides a moisturizing and wrinkle-reducing composition containing recombinant collagen, which includes the following active ingredients by weight: 0.75 parts of unsaponifiable matter of avocado tree fat, 1.5 parts of sodium hyaluronate, 1.5 parts of palmitoyl tripeptide-1, and 0.1 parts of recombinant collagen. The preparation method is to mix the commercially available raw materials evenly.

[0089] Comparative Example 2

[0090] This comparative example provides a moisturizing and wrinkle-reducing composition containing recombinant collagen, which includes the following active ingredients by weight: 1.5 parts of dipalmitoyl hydroxyproline, 1.5 parts of sodium hyaluronate, 11.5 parts of palmitoyl tripeptide-1, and 0.1 parts of recombinant collagen. The preparation method is to mix the commercially available raw materials evenly.

[0091] Comparative Example 3

[0092] This comparative example provides a moisturizing and wrinkle-reducing composition containing recombinant collagen, which includes the following active ingredients by weight: 1.5 parts of dipalmitoyl hydroxyproline, 0.75 parts of unsaponifiable matter of avocado oil, 1.5 parts of palmitoyl tripeptide-1, and 0.1 parts of recombinant collagen. The preparation method is to mix the commercially available raw materials evenly.

[0093] Comparative Example 4

[0094] This comparative example provides a moisturizing and wrinkle-reducing composition containing recombinant collagen, which includes the following active ingredients by weight: 1.5 parts of dipalmitoyl hydroxyproline, 0.75 parts of unsaponifiable matter of avocado oil, 1.5 parts of sodium hyaluronate, and 0.1 parts of recombinant collagen. The preparation method is to mix the commercially available raw materials evenly.

[0095] Comparative Example 5

[0096] This comparative example provides a moisturizing and wrinkle-reducing composition, which includes the following active ingredients by weight: 1.5 parts of dipalmitoyl hydroxyproline, 0.75 parts of avocado unsaponifiable matter, 1.5 parts of sodium hyaluronate, and 1.5 parts of palmitoyl tripeptide-1. The preparation method is to mix the commercially available raw materials uniformly.

[0097] Application Example 1

[0098] This application example provides a cream with moisturizing and wrinkle-lightening effects, and its formula is as follows:

[0099]

[0100]

[0101] Its preparation method is:

[0102] (1) Add the raw materials of phase A into a clean water phase pot, stir and heat to 80°C, and keep warm for 15 minutes;

[0103] (2) Add the raw materials of phase B into a clean oil phase pot, stir and heat to 80°C, and keep warm for 15 minutes;

[0104] (3) After the insulation is completed, phase A and phase B are pumped into the emulsifier, homogenized at 2000 r / min, phase C is added, homogenized at 2000 r / min, the temperature is lowered to 45°C, phase D is added, and stirred evenly;

[0105] (4) Take samples for observation and testing, and discharge the materials after they pass the test.

[0106] Application Example 2

[0107] This application example provides a cream with moisturizing and wrinkle-lightening effects, and its formula is as follows:

[0108]

[0109]

[0110] Its preparation method is:

[0111] (1) Add the raw materials of phase A into a clean water phase pot, stir and heat to 75°C, and keep warm for 15 minutes;

[0112] (2) Add the Phase B ingredients into a clean oil phase pot, stir and heat to 75°C, and keep warm for 15 minutes;

[0113] (3) After the insulation is completed, phase A and phase B are pumped into the emulsifier, homogenized at 2000 r / min, phase C is added, homogenized at 2000 r / min, the temperature is lowered to 40°C, phase D is added, and stirred evenly;

[0114] (4) Take samples for observation and testing, and discharge the materials after they pass the test.

[0115] Application Example 3

[0116] This application example provides a cream with moisturizing and wrinkle-lightening effects, and its formula is as follows:

[0117]

[0118]

[0119] Its preparation method is:

[0120] (1) Add the raw materials of phase A into a clean water phase pot, stir and heat to 85°C, and keep warm for 15 minutes;

[0121] (2) Add the Phase B ingredients into a clean oil phase pot, stir and heat to 85°C, and keep warm for 15 minutes;

[0122] (3) After the insulation is completed, phase A and phase B are pumped into the emulsifier, homogenized at 2000 r / min, phase C is added, homogenized at 2000 r / min, the temperature is lowered to 50°C, phase D is added, and stirred evenly;

[0123] (4) Take samples for observation and testing, and discharge the materials after they pass the test.

[0124] Application Example 4

[0125] This application example provides a cream with moisturizing and wrinkle-lightening effects. The formula of the cream is different from that of application example 1 only in that the composition prepared in example 1 is replaced by the composition prepared in example 4, and the other raw materials and contents remain unchanged. The preparation method thereof is similar to that of application example 1.

[0126] Comparative Application Examples 1-5

[0127] This comparative application example provides five creams with moisturizing and wrinkle-lightening effects. The difference between the formulas of these five creams and those of application example 1 is that the composition prepared in example 1 is replaced by the compositions prepared in comparative examples 1-5 in equal amounts, and the other raw materials and contents remain unchanged. The preparation method thereof is referred to application example 1.

[0128] Application Example 5

[0129] This application example provides a cream with moisturizing and wrinkle-lightening effects. The formula of this cream is different from that of application example 1 only in that phase D is 8% of the composition prepared in Example 1, and does not contain allantoin, β-glucan, rice fermentation product filtrate and glycerol glucoside, and the raw materials and contents of other phases remain unchanged. The preparation method thereof is referred to application example 1.

[0130] Comparative Application Example 6

[0131] This comparative application example provides a cream with moisturizing and wrinkle-reducing effects. The raw materials for its preparation differ from those in application example 1 only in that phase D contains 2% allantoin, 2% β-glucan, 2% rice fermentation product filtrate, and 2% glycerol glucoside, and does not contain the composition prepared in example 1. The raw materials and contents of other phases remain unchanged. The preparation method thereof refers to application example 1.

[0132] Test Example 1

[0133] Skin safety test:

[0134] The cream products prepared in Application Examples 1-5 were placed in a patch tester, and the patch tester with the test substance added was attached to the flexion side of the forearm of the test persons (30 people), and the patch was lightly pressed with the palm to evenly apply it on the skin for 24 hours; the skin irritation and sensitization were observed according to Table 1 (Skin Reaction Grading Standard for Skin Closure Patch Test) 30 minutes, 24 hours, and 48 hours after the removal of the test substance patch tester, and the observation results were recorded.

[0135] Table 1

[0136]

[0137] The results showed that no adverse skin reactions occurred in the products of Application Examples 1-5, indicating that the moisturizing and wrinkle-reducing composition and cream containing recombinant collagen of the present invention are safe and non-irritating to the skin.

[0138] Test Example 2

[0139] Moisturizing and wrinkle-lightening effect test:

[0140] 1) Detection method

[0141] 110 healthy Chinese female subjects with obvious lip lines and relatively dry lip skin were selected (randomly divided into 11 groups, 10 subjects in each group), with an age range of 18-60 years old and an average age of 31 years old.

[0142] The stratum corneum moisture content and the number / area of ​​lip wrinkles were tested before the subjects used the test product (D0), 20 minutes after using the product (20 minutes), after using the product for 5 days (D5), and after using the product for 11 days (D11). In the long-term effect tests D5 and D11, the subjects used the product once a day at 8:00 and 20:00, of which D5 was tested before using the product at 8:00 on the sixth day, and D11 was tested before using the product at 8:00 on the 12th day.

[0143] Data collection was arranged for the subjects before using the product and 20 minutes, 5 days, and 11 days after use. Skin parameters and images were collected using a skin tester each time.

[0144] 2) Testing environment

[0145] The test environment was 21°C and 50% humidity, which met the design requirements.

[0146] 3) Test equipment

[0147] Skin fast optical imaging system Evaskin-S5 (EOTECH, France)

[0148] Skin stratum corneum moisture content tester CM 825 (Courage & Khazaka, Germany)

[0149] 4) Test results

[0150] 4.1) Table 1 shows the average moisture content of the stratum corneum of the lip skin of each group, and Table 2 shows the change analysis of the moisture content of the stratum corneum of the lip skin of each group

[0151] Table 1

[0152]

[0153] Table 2

[0154]

[0155]

[0156] According to the data in Tables 1-2, the moisturizing and wrinkle-reducing composition containing recombinant collagen involved in the present invention can effectively increase the moisture content of the skin's stratum corneum. After using the product of Application Example 1 for 20 minutes, the moisture content of the stratum corneum of the lip skin is increased by 90.61% compared with before use; after using the product of Application Example 1 for 5 days, the moisture content of the stratum corneum of the lip skin is increased by 50.14% compared with before use; after using the product of Application Example 1 for 11 days, the moisture content of the stratum corneum of the lip skin is increased by 90.80% compared with before use.

[0157] Comparing Application Example 1 with Application Example 4, it can be seen that the recombinant collagen from a specific source involved in the present invention, when combined with dipalmitoyl hydroxyproline, avocado unsaponifiable matter, sodium hyaluronate, and palmitoyl tripeptide-1, has a better moisturizing effect.

[0158] By comparing Application Example 1 with Comparative Application Examples 1-5, it can be seen that the dipalmitoyl hydroxyproline, avocado unsaponifiable matter, sodium hyaluronate, palmitoyl tripeptide-1 and recombinant collagen in the moisturizing and wrinkle-reducing composition containing recombinant collagen of the present invention cooperate with each other and have a synergistic effect in moisturizing.

[0159] By comparing Application Example 1 with Application Example 5 and Comparative Application Example 6, it can be seen that in the cream with moisturizing and wrinkle-reducing effects of the present invention, the skin conditioner and the moisturizing and wrinkle-reducing composition containing recombinant collagen play a synergistic role in moisturizing.

[0160] 4.2) Table 3 shows the average number of lip wrinkles in each group, and Table 4 shows the change analysis of the number of lip wrinkles in each group

[0161] Table 3

[0162]

[0163]

[0164] Table 4

[0165]

[0166]

[0167] According to the data in Tables 3-4, the moisturizing and wrinkle-reducing composition containing recombinant collagen involved in the present invention can effectively reduce the number of lip wrinkles. After using the product of Application Example 1 for 20 minutes, the number of lip wrinkles decreased by 66.67% compared with before use; after using the product of Application Example 1 for 5 days, the number of lip wrinkles decreased by 20.00% compared with before use; after using the product of Application Example 1 for 11 days, the number of lip wrinkles decreased by 60.00% compared with before use.

[0168] Comparing Application Example 1 with Application Example 4, it can be seen that the recombinant collagen from a specific source involved in the present invention, when combined with dipalmitoyl hydroxyproline, avocado unsaponifiable matter, sodium hyaluronate, and palmitoyl tripeptide-1, has a better effect in moisturizing and reducing wrinkles.

[0169] By comparing Application Example 1 with Comparative Application Examples 1-5, it can be seen that the dipalmitoyl hydroxyproline, avocado unsaponifiable matter, sodium hyaluronate, palmitoyl tripeptide-1 and recombinant collagen in the moisturizing and wrinkle-reducing composition containing recombinant collagen of the present invention cooperate with each other and have a synergistic effect in reducing wrinkles.

[0170] By comparing Application Example 1 with Application Example 5 and Comparative Application Example 6, it can be seen that in the cream with moisturizing and wrinkle-reducing effects of the present invention, the skin conditioner and the moisturizing and wrinkle-reducing composition containing recombinant collagen play a synergistic role in reducing wrinkles.

[0171] 4.3) Table 5 shows the average values ​​of lip wrinkle areas in each group, and Table 6 shows the changes in lip wrinkle areas in each group

[0172] Table 5

[0173]

[0174]

[0175] Table 6

[0176]

[0177]

[0178] According to the data in Tables 5-6, the moisturizing and wrinkle-reducing composition containing recombinant collagen involved in the present invention can effectively reduce the number of lip wrinkles. After using the product of Application Example 1 for 20 minutes, the area of ​​lip wrinkles decreased by 75.27% compared with before use; after using the product of Application Example 1 for 5 days, the area of ​​lip wrinkles decreased by 17.88% compared with before use; after using the product of Application Example 1 for 11 days, the area of ​​lip wrinkles decreased by 49.55% compared with before use.

[0179] Comparing Application Example 1 with Application Example 4, it can be seen that the recombinant collagen from a specific source involved in the present invention, when combined with dipalmitoyl hydroxyproline, avocado unsaponifiable matter, sodium hyaluronate, and palmitoyl tripeptide-1, has a better effect in reducing wrinkles.

[0180] By comparing Application Example 1 with Comparative Application Examples 1-5, it can be seen that the dipalmitoyl hydroxyproline, avocado unsaponifiable matter, sodium hyaluronate, palmitoyl tripeptide-1 and recombinant collagen in the moisturizing and wrinkle-reducing composition containing recombinant collagen of the present invention cooperate with each other and have a synergistic effect in reducing wrinkles.

[0181] By comparing Application Example 1 with Application Example 5 and Comparative Application Example 6, it can be seen that in the cream with moisturizing and wrinkle-reducing effects of the present invention, the skin conditioner and the moisturizing and wrinkle-reducing composition containing recombinant collagen play a synergistic role in reducing wrinkles.

[0182] The applicant declares that the present invention illustrates the technical solution of the present invention through the above-mentioned embodiments, but the present invention is not limited to the above-mentioned embodiments, that is, it does not mean that the present invention must rely on the above-mentioned embodiments to be implemented. Those skilled in the art should understand that any improvement of the present invention, equivalent replacement of various raw materials of the product of the present invention, addition of auxiliary components, selection of specific methods, etc., all fall within the protection scope and disclosure scope of the present invention.

[0183] The preferred embodiments of the present invention are described in detail above. However, the present invention is not limited to the specific details in the above embodiments. Within the technical concept of the present invention, a variety of simple modifications can be made to the technical solution of the present invention, and these simple modifications all belong to the protection scope of the present invention.

[0184] It should also be noted that the various specific technical features described in the above specific embodiments can be combined in any suitable manner without contradiction. In order to avoid unnecessary repetition, the present invention will not further describe various possible combinations.

Claims

1. A moisturizing and wrinkle-lightening composition containing recombinant collagen, characterized in that: The moisturizing and wrinkle-reducing composition containing recombinant collagen comprises dipalmitoyl hydroxyproline, unsaponifiable matter of avocado butter, sodium hyaluronate, palmitoyl tripeptide-1 and recombinant collagen.

2. The moisturizing and wrinkle-lightening composition containing recombinant collagen according to claim 1, characterized in that: The nucleotide sequence encoding the recombinant collagen is the sequence shown in SED ID NO:

1.

3. The moisturizing and wrinkle-lightening composition containing recombinant collagen according to claim 1 or 2, characterized in that: The moisturizing and wrinkle-reducing composition containing recombinant collagen comprises, by weight, 1-5 parts of dipalmitoyl hydroxyproline, 0.1-3 parts of unsaponifiable matter of avocado fat, 1-5 parts of sodium hyaluronate, 1-5 parts of palmitoyl tripeptide-1 and 0.01-1 parts of recombinant collagen.

4. Use of the moisturizing and wrinkle-lightening composition containing recombinant collagen according to any one of claims 1 to 3 in the preparation of cosmetics; Preferably, the cosmetic is applied to the eyes, face and / or lips; Preferably, the mass percentage of the moisturizing and wrinkle-reducing composition containing recombinant collagen in the cosmetics is 3-10%.

5. A cream with moisturizing and wrinkle-lightening effects, characterized in that: The raw materials for preparing the cream include, by mass percentage: 3-10% of the moisturizing and wrinkle-reducing composition containing recombinant collagen according to claim 1 or 2, 5-20% of a moisturizer, 0.5-2% of an antioxidant, 0.5-2% of a skin feel regulator, 1-6% of an emulsifier, 15-50% of a moisturizer, 0.1-4% of a skin conditioner and 4-80% of water.

6. The cream with moisturizing and wrinkle-lightening effect according to claim 5, characterized in that: The skin conditioning agent includes any one of allantoin, β-glucan, rice fermentation product filtrate or glyceryl glucoside or a combination of at least two thereof.

7. The cream with moisturizing and wrinkle-lightening effect according to claim 5 or 6, characterized in that: The moisturizing agent includes any one or a combination of at least two of glycerin, panthenol, fructose, butylene glycol or 1,2-hexanediol; Preferably, the antioxidant comprises any one of tocopherol acetate, pentaerythritol tetrakis (di-tert-butyl hydroxyhydrocinnamate) or p-hydroxyacetophenone, or a combination of at least two thereof.

8. The cream with moisturizing and wrinkle-lightening effect according to any one of claims 5 to 7, characterized in that: The skin feel regulator includes any one or a combination of at least two of sodium acrylate / sodium acryloyldimethyl taurate copolymer, isohexadecane, polysorbate-80 or sorbitan isostearate; Preferably, the emulsifier includes any one or a combination of at least two of cetyl alcohol, glyceryl stearate, PEG-75 stearate, ceteth-20, steareth-20 or steareth-21; Preferably, the emollient comprises any one of C10-18 fatty acid triglycerides, avocado butter, petrolatum, squalane, cocoa seed butter, white beeswax, cetearyl alcohol, batyl alcohol, phytosteryl canola oil ester or polydimethylsiloxane, or a combination of at least two thereof.

9. The method for preparing the cream with moisturizing and wrinkle-lightening effects according to any one of claims 5 to 8, characterized in that: The preparation method comprises: (1) mixing water and a moisturizer, heating and keeping the temperature, to obtain a phase A; mixing an emulsifier, a moisturizer and an antioxidant, heating and keeping the temperature, to obtain a phase B; (2) Mixing phase A and phase B, homogenizing, adding a skin feel regulator, homogenizing, cooling, adding a skin conditioner and a moisturizing and wrinkle-reducing composition containing recombinant collagen, and mixing to obtain the cream having moisturizing and wrinkle-reducing effects.

10. The method for preparing the cream with moisturizing and wrinkle-lightening effect according to claim 9, characterized in that: The temperatures of the heating and insulation are independently selected from 75-85°C; Preferably, the cooling temperature is 40-50°C; Preferably, the homogenization speed is independently 1000-3000 r / min.

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