Industrial preparation system and control method of enzymatic casein polypeptide

Abstract

The present application provides an industrial preparation system for enzymatic casein polypeptide and a control method thereof, wherein the preparation system comprises one or more enzymatic hydrolysis tanks and one or more first external circulation pipelines, wherein the enzymatic hydrolysis tank comprises a heat exchange jacket and a differential stirring device, wherein the differential stirring device comprises a main shaft, a plurality of main propellers, a plurality of secondary shafts, a plurality of secondary propellers and a motor. The rotation speeds of the main shaft and the secondary shaft are different to form a differential rotation for slowing down the formation of casein micelles. The two ends of the first external circulation pipeline are respectively connected to the bottom and the upper part of the enzymatic hydrolysis tank so that the material in the enzymatic hydrolysis tank is drawn out from the bottom of the enzymatic hydrolysis tank and then passed into the enzymatic hydrolysis tank from the upper part of the enzymatic hydrolysis tank, forming a circulation of the material in the upper and lower directions of the enzymatic hydrolysis tank and outside to improve the enzymatic hydrolysis efficiency. The preparation system can effectively enhance the fluidity of the material in the enzymatic hydrolysis tank, slow down the formation of casein micelles and increase the enzymatic hydrolysis efficiency by setting the main shaft and secondary shaft with different rotation speeds and the first external circulation pipeline.

Description

Technical Field

[0001] The present application relates to the technical field of enzymology devices, and in particular to an industrial preparation system for enzymatic casein polypeptides and a control method thereof. Background Art

[0002] Milk protein is a general term for a mixture of various proteins in the milk system. Milk protein is mainly composed of casein and whey protein. Among them, casein can be used to make cheese. The main consumption forms of cheese are original cheese, processed cheese and cheese products mainly for snacks, and its market size is relatively small. In contrast, the market for formula milk powder is vast and the consumption of whey protein is huge. Based on the above objective reasons, casein has become an important by-product of the deep processing industry of dairy products, and the high value-added application of casein has become a major strategic project for the future of the dairy industry.

[0003] The small molecule peptides produced by casein hydrolysis have multiple functions in the body, such as participating in immune regulation, scavenging free radicals, regulating blood pressure balance, anti-thrombosis and strengthening bone health. Casein is low in allergenicity and easily absorbable, so it has high nutritional value and market value. However, since the hydrolysis process of casein is very unstable, it is very easy to form casein micelle precipitation. At the same time, casein hydrolysis will lead to the exposure of a large number of hydrophobic amino acids, resulting in a bitter taste of the hydrolyzate. Any carelessness in the production process will make the casein final product unusable. Therefore, in the production of casein enzymatic casein hydrolysis (enzymatic hydrolysis: hydrolysis under the action of protease), the taste, molecular weight distribution, polypeptide content and other indicators of enzymatic casein polypeptides must have a high degree of batch-to-batch stability in industrial applications.

[0004] However, in the current large-scale industrial production process of enzymatic casein, there is no casein polypeptide-specific enzymatic hydrolysis system, and the existing enzymatic hydrolysis equipment has many problems such as low control accuracy of reaction conditions such as pH value and temperature, poor circulation and stirring shear force in the tank, and slow enzyme inactivation and heating. Specifically, the general enzymatic hydrolysis equipment commonly used in the existing process scheme mainly has the following problems: 1. During the enzymatic hydrolysis process, due to the mutual aggregation of caseins, it is easy to form larger casein micelles, which will produce a series of problems such as agglomeration, precipitation, and wall hanging, resulting in reduced enzymatic hydrolysis efficiency, poor product solubility, and large fluctuations in hydrolysis degree and molecular weight distribution between batches. 2. During the pH adjustment process, the amount of acid and alkali added is unevenly distributed, which can easily cause the local pH of the solution system to be too high or too low, resulting in casein denaturation and precipitation. 3. The enzymatic reaction efficiency is low. Since the substrate concentration of the enzymatic hydrolysis solution is generally between 10% and 20%, the traditional enzymatic hydrolysis system mainly relies on the disturbance generated by the stirring paddle in the tank to react the enzyme with the substrate, and the enzymatic efficiency is low and the amount of enzyme used is large. 4. The system has a slow heating rate. The existing system generally takes more than 30 minutes to heat up from the enzymatic hydrolysis temperature of 60°C to the enzyme inactivation temperature of 90°C. Since the overall enzymatic hydrolysis time is generally around 40 minutes, a longer heating time can easily lead to the inability to guarantee the stability of enzymatic casein polypeptide products between batches.

[0005] Based on the above-mentioned problems of the prior art, it is urgent to optimize the existing preparation system to adapt to the industrial production of large-scale enzymatic casein, and at least improve some of the technical problems of the above-mentioned prior art. Summary of the invention

[0006] This application is made in view of the above-mentioned state of the prior art. The purpose of this application is to provide an industrial preparation system of enzymatic casein polypeptides to reduce the generation of casein micelles in the enzymatic casein process and improve the enzymatic efficiency.

[0007] The present application also provides a control method for the above-mentioned enzymatic casein polypeptide industrial preparation system.

[0008] The present application provides an industrial preparation system for enzymatic casein polypeptides, which comprises one or more enzymatic hydrolysis tanks and one or more first external circulation pipelines, wherein the enzymatic hydrolysis tank comprises a heat exchange jacket and a differential stirring device, wherein the heat exchange jacket is arranged on the wall surface of the enzymatic hydrolysis tank for regulating the temperature of the material in the enzymatic hydrolysis tank, and the differential stirring device comprises a main shaft, a plurality of main propellers, a plurality of secondary shafts, a plurality of secondary propellers and a motor, wherein the plurality of main propellers are connected to the main shaft, and the plurality of secondary propellers are connected to the secondary shaft; the motor is connected to the main shaft or one of the secondary shafts, and at least one of the The secondary shaft is connected to the main shaft in a transmission manner, so that one motor drives the main shaft and the secondary shaft to rotate. The rotation speeds of the main shaft and the secondary shaft are different to form a differential rotation for slowing down the formation of casein micelles. The two ends of the first external circulation pipeline are respectively connected to the bottom and the top of the enzymolysis tank. The first external circulation pipeline is provided with a first circulation pump to allow the material in the enzymolysis tank to be extracted from the bottom of the enzymolysis tank and then passed into the enzymolysis tank from the top of the enzymolysis tank, thereby forming a circulation of the material in the upper and lower directions and outside of the enzymolysis tank to improve the enzymolysis efficiency.

[0009] In at least one possible embodiment, the motor is connected to the main shaft, and the secondary shaft is connected to the main shaft through a connecting rod, so that the secondary shaft can rotate around the main shaft, and the differential stirring device includes a main wheel, a secondary wheel and a transmission belt or a transmission chain, the main wheel is connected to the main shaft, and the secondary wheel is connected to the secondary shaft, and the transmission belt is sleeved on the main wheel and the secondary wheel, so that the secondary wheel rotates with the rotation of the main wheel, the diameter of the main wheel is larger than the diameter of the secondary wheel, so that the self-rotation speed of the main shaft is lower than the self-rotation speed of the secondary shaft, and the main shaft is arranged closer to the central axis of the enzymatic hydrolysis tank than the secondary shaft.

[0010] In at least one possible embodiment, the differential stirring device further includes a scraper, which is connected to the main shaft and rotates with the main shaft, and the scraper is close to the inner wall of the enzymolysis tank to clean the material on the inner wall of the enzymolysis tank.

[0011] In at least one possible embodiment, the first external circulation pipeline is also provided with a pipeline extrusion impactor, which includes a joint, a reducer, an extrusion head and a spoiler groove. The joints are arranged at both ends of the pipeline extrusion impactor to connect pipelines. The reducer is arranged on the inner side of the joint. The diameter of at least part of the reducer is variable. The extrusion head is arranged on the inner side of the reducer and has a pipe mouth capable of extruding materials. The spoiler groove is arranged in the middle of the pipeline extrusion impactor.

[0012] In at least one possible embodiment, the industrial preparation system of enzymatic casein polypeptide further comprises a second external circulation pipeline, the second external circulation pipeline is provided with a second circulation pump and an external heat exchanger, and the two ends of the second external circulation pipeline are respectively connected to the bottom and the upper part of the enzymolysis tank, so that the material in the enzymolysis tank is extracted from the bottom of the enzymolysis tank, the temperature of the material is regulated by the external heat exchanger, and then the material is passed into the enzymolysis tank from the upper part of the enzymolysis tank.

[0013] In at least one possible embodiment, the industrial preparation system of enzymatic casein polypeptide comprises a plurality of the enzymatic hydrolysis tanks and a plurality of the first external circulation pipelines of the same number, the plurality of first external circulation pipelines are each connected to one of the enzymatic hydrolysis tanks, the plurality of enzymatic hydrolysis tanks are connected in parallel, and the plurality of enzymatic hydrolysis tanks are each connected to one of the second external circulation pipelines.

[0014] In at least one possible embodiment, the industrial preparation system of enzymatic casein polypeptide further comprises an acid-base configuration device, wherein the acid-base configuration device is connected to the enzymolysis tank, and a three-fluid atomizing nozzle is provided at the end of the pipeline connecting the acid-base configuration device to the enzymolysis tank, wherein the three-fluid atomizing nozzle comprises an inner air tube, an acid-base liquid tube and an outer air tube, wherein the inner air tube is located radially inwardly of the acid-base liquid tube, the acid-base liquid tube is located radially inwardly of the outer air tube, and a swirl sheet is provided at the end of the inner air tube to form a gas swirl, wherein the acid-base liquid tube can be introduced into the acid-base liquid tube, and the air can be introduced into the inner air tube and the outer air tube, so that the acid or the alkali liquid can form atomized droplets under the action of the airflow.

[0015] In at least one possible embodiment, the industrial preparation system of enzymatic casein polypeptide further includes a sterilization device and one or more enzyme adding tanks, wherein the sterilization device is connected to the enzymolysis tank to pass the sterilized casein solution into the enzymolysis tank, and the enzyme adding tank is connected to the enzymolysis tank to pass the enzyme preparation into the enzymolysis tank.

[0016] The present application provides a control method for an industrial preparation system of enzymatic casein polypeptides, which is applied to an industrial preparation system of enzymatic casein polypeptides as described above. During the enzymatic hydrolysis of casein, the differential stirring device of the enzymatic hydrolysis tank and the first external circulation pipeline operate simultaneously for at least part of the time to allow the material in the enzymatic hydrolysis tank to flow.

[0017] The present application also provides a control method for an industrial preparation system of enzymatic casein polypeptides, which is applied to the above-mentioned industrial preparation system of enzymatic casein polypeptides. During the enzyme inactivation process of enzymatic casein, the heat exchange jacket of the enzymatic hydrolysis tank and the second external circulation pipeline are operated simultaneously to heat up the material in the enzymatic hydrolysis tank.

[0018] The industrial preparation system and control method of enzymatic casein polypeptide provided by the present application adopts a differential stirring device in the enzymatic hydrolysis tank to cooperate with the first external circulation pipeline connected to the enzymatic hydrolysis tank, which can effectively enhance the fluidity of the material in the enzymatic hydrolysis tank, thereby enhancing the shear force on casein, slowing down the formation of casein micelles, and reducing or avoiding the formation of larger casein micelles in the enzymatic hydrolysis tank. At the same time, the technical solution can also improve the enzymatic hydrolysis efficiency of the enzymatic hydrolysis tank, thereby reducing the amount of enzyme used and improving the production efficiency of casein polypeptide products. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] Figure 1 It is a schematic structural diagram of a preparation system according to one embodiment of the present application.

[0020] Figure 2 It is a schematic structural diagram of an enzyme addition tank according to one embodiment of the present application.

[0021] Figure 3 It is a schematic structural diagram of an acid-base configuration device according to one embodiment of the present application.

[0022] Figure 4 It is a schematic diagram of the structure of an enzymatic hydrolysis tank group according to one embodiment of the present application.

[0023] Figure 5 It is a schematic diagram of the structure of an enzymatic hydrolysis tank according to one embodiment of the present application.

[0024] Figure 6 It is a schematic diagram of the internal structure of an enzymolysis tank according to one embodiment of the present application.

[0025] Figure 7 It is a schematic diagram of the partial structure of an enzymolysis tank according to one embodiment of the present application.

[0026] Figure 8 It is a schematic diagram of the partial structure of the enzymolysis tank from another perspective according to one embodiment of the present application.

[0027] Fig. 9 It is a schematic structural diagram of a pipeline extrusion impactor according to one embodiment of the present application.

[0028] Fig.10 It is a schematic structural diagram of a three-fluid atomizing nozzle according to one embodiment of the present application.

[0029] Fig.11 It is a schematic diagram of the partial structure of a three-fluid atomizing nozzle according to one embodiment of the present application.

[0030] Fig.12 This is a photo of the material status of the first embodiment.

[0031] Fig.13This is a photo of the material status of the second embodiment.

[0032] Fig.14 This is a photo of the material status of the third embodiment.

[0033] Fig.15 This is a photo of the material status of Comparative Example 1.

[0034] Fig.16 This is a photo of the material status of Comparative Example 2.

[0035] Fig.17 This is another material state photo of the first embodiment.

[0036] Fig.18 This is another material state photo of the second embodiment.

[0037] Fig.19 This is another material state photo of the third embodiment.

[0038] Fig. 20 This is another material state photo of Comparative Example 1.

[0039] Fig.21 This is another material state photo of Comparative Example 2.

[0040] Description of Reference Numerals

[0041] 10 Sterilization equipment

[0042] 20 Enzyme Addition Tank

[0043] 30 Acid and alkali preparation equipment

[0044] 31 Concentrated acid tank

[0045] 32 concentrated alkali tank

[0046] 33 Alkali tank

[0047] 34 Dilute Acid Tank

[0048] 40 Enzyme Hydrolysis Tanks

[0049] 41 Heat exchange jacket

[0050] 42 Differential stirring device

[0051] 421 Spindle

[0052] 422 Main propeller

[0053] 423 Countershaft

[0054] 424 secondary propeller

[0055] 425 Motor

[0056] 426 Main Wheel

[0057] 427 Secondary wheel

[0058] 428 Transmission Belt

[0059] 429 Scraper

[0060] 50 External heat exchanger

[0061] 61 The first external circulation pipeline

[0062] 611 Pipeline Extrusion Impactor

[0063] 6111 Connector

[0064] 6112 Reducer

[0065] 6113 Extrusion Head

[0066] 6114 Spoiler Slot

[0067] 62 Second external circulation pipeline

[0068] 71 First circulation pump

[0069] 72 Second circulation pump

[0070] 80 Three-fluid atomizing nozzle

[0071] 81 Air inner tube

[0072] 82 Acid and alkali liquid pipe

[0073] 83 Air outer tube

[0074] 84 Swirl sheet DETAILED DESCRIPTION

[0075] The exemplary embodiments of the present application are described below with reference to the accompanying drawings. It should be understood that these specific descriptions are only used to teach those skilled in the art how to implement the present application, and are not intended to exhaust all possible methods of the present application, nor to limit the scope of the present application.

[0076] The connection referred to in this application includes direct connection and indirect connection through pipelines, lines, transmission parts, etc.

[0077] The embodiments of the present application provide an industrial preparation system for enzymatic casein polypeptide (hereinafter, sometimes referred to as "preparation system"), such as Figure 1 As shown, the preparation system may include a sterilization device 10 , an enzyme adding tank 20 , an acid-base preparation device 30 and one or more enzymolysis tanks 40 .

[0078] The sterilizing device 10 may be a pasteurizing device. It is understood that the pasteurizing device is suitable for sterilizing dairy products and emulsion byproducts. Figure 1 As shown, the sterilization device 10 can be connected to the enzymolysis tank 40 through a pipeline, and the casein solution can enter the enzymolysis tank 40 after being (pasteurized) sterilized by the sterilization device 10.

[0079] like Figure 1 and Figure 2 As shown, the preparation system may include one or more enzyme adding tanks 20. Exemplarily, two enzyme adding tanks 20 are provided in the present embodiment. A plurality of enzyme adding tanks 20 may be connected in parallel. Enzyme preparation and water may be added to the enzyme adding tank 20 so that the enzyme preparation is fully dissolved in the enzyme adding tank 20. It is understood that the water used in the present embodiment may be reverse osmosis water (i.e., RO water, which is filtered by reverse osmosis through a semipermeable membrane to remove most of the impurities such as ions and microorganisms in the water). The enzyme adding tank 20 may be connected to the enzymolysis tank 40 by a pump (particularly a centrifugal pump) to transport the dissolved enzyme preparation. The enzyme adding tank 20 may also be provided with a high liquid level indicator (LSH), a low liquid level indicator (LSL), a weight transmitter (WT), etc.

[0080] like Figure 1 and Figure 3 As shown, the acid-base configuration device 30 may include a concentrated acid tank 31, a concentrated alkali tank 32, a diluted alkali tank 33 and a diluted acid tank 34. Reverse osmosis water and nitric acid solution may be added to the concentrated acid tank 31, and reverse osmosis water and food-grade sodium hydroxide (solid) may be added to the concentrated alkali tank 32, and they may be fully stirred and dissolved. The concentrated acid tank 31 may be connected to the diluted acid tank 34 by a pump (particularly a diaphragm pump), and the concentrated alkali tank 32 may be connected to the diluted alkali tank 33 by a pump (particularly a diaphragm pump), so that the concentrated acid solution and the concentrated alkali solution are respectively passed into the diluted acid tank 34 and the diluted alkali tank 33 for accurate configuration. The concentrated acid tank 31 and the concentrated alkali tank 32 may be provided with a weight transmitter (WT) respectively.

[0081] The diluted alkali tank 33 and the diluted acid tank 34 can be connected to the enzymolysis tank 40 to adjust the pH value (acidity and alkalinity) of the enzymolysis solution in the enzymolysis tank 40. The diluted alkali tank 33 and the diluted acid tank 34 can be respectively fed with reverse osmosis water, and are respectively provided with a high liquid level indicator (LSH), a low liquid level indicator (LSL), a weight transmitter (WT) and a conductivity transmitter (CT, for measuring ion concentration) to achieve precise pH value regulation. The diluted alkali tank 33 and the diluted acid tank 34 can be respectively connected to the enzymolysis tank 40 through a pump (especially a centrifugal pump), and a flow meter (especially an electromagnetic flow meter) can also be provided in the connecting pipeline to measure the flow of the acid solution and the alkali solution respectively.

[0082] like Figure 1 , Figure 4 and Figure 5As shown, the enzymolysis tank 40 may be provided with a heat exchange jacket 41 and a differential stirring device 42. The heat exchange jacket 41 may be provided on the wall of the enzymolysis tank 40 to regulate the temperature of the material in the enzymolysis tank 40. Preferably, the heat exchange jacket 41 may be a steam heating jacket, and the temperature in the enzymolysis tank 40 may be increased by passing steam into the heat exchange jacket 41. The heat exchange jacket 41 may be provided with a steam inlet (LS) and a condensate outlet (SC), which are used to pass steam and discharge steam condensate, respectively. The differential stirring device 42 may stir the material in the enzymolysis tank 40 by means of a plurality of stirring paddles with different speeds ( Figure 5 The symbol represents the differential stirring device). The enzymolysis tank 40 may be provided with a high liquid level indicator (LSH), a low liquid level indicator (LSL), a pH value transmitter (PH) and a temperature transmitter (TT). A plurality of enzymolysis tanks 40 may be connected in parallel to form an enzymolysis tank group. For example, in the present embodiment, three enzymolysis tanks 40 are provided in parallel. It can be understood that the enzymolysis tanks 40 provided in parallel may share some pipelines.

[0083] Specifically, Figure 6 , Figure 7 and Figure 8 As shown, the differential stirring device 42 may include a main shaft 421, a main propeller 422, a secondary shaft 423, a secondary propeller 424, a motor 425, a main wheel 426, a secondary wheel 427 and a transmission belt 428. The central shaft 421 may be connected to a plurality of main propellers 422. For example, the central shaft 421 in this embodiment is connected to a total of 10 main propellers 422 (including 5 groups of main propellers arranged at intervals, and 1 main propeller group includes 2 main propellers). The secondary shaft 423 may be connected to a plurality of secondary propellers 424. For example, the secondary shaft 423 in this embodiment is connected to a total of 8 secondary propellers 424 (including 4 groups of secondary propellers arranged at intervals, and 1 secondary propeller group includes 2 secondary propellers). The motor 425 may be connected to the main shaft 421 to drive the main shaft 421 to rotate. The secondary shaft 423 may be connected to the primary shaft 421 via a connecting rod, so that when the primary shaft 421 rotates, the secondary shaft 423 may rotate around the primary shaft 421 (ie, the secondary shaft 423 may perform an orbital motion around the primary shaft 421 ).

[0084] The main wheel 426 can be arranged on the main shaft 421, and the main wheel 426 can rotate together with the main shaft 421. The secondary wheel 427 can be arranged on the secondary shaft 423, and the secondary wheel 427 can rotate together with the secondary shaft 423. The main wheel 426 and a secondary wheel 427 can be connected by a transmission belt 428, and the transmission belt 428 can be sleeved on the main wheel 426 and the secondary wheel 427, so that the secondary wheel 427 rotates together with the main wheel 426, thereby realizing the rotation of the main shaft 421 and the secondary shaft 423 together, that is, the secondary shaft 423 rotates around its own central axis. It can be understood that the rotation of the secondary shaft 423 is driven by the transmission belt 428, and the revolution of the secondary shaft 423 around the main shaft 421 is driven by the connecting rod between the main shaft 421 and the secondary shaft 423. Canceling the transmission belt 428 can make the secondary shaft 423 only perform the revolution around the main shaft 421 through the connecting rod, and no longer perform the rotation.

[0085] The number of the secondary shaft 423, the secondary wheel 427 and the transmission belt 428 can be multiple (two or more). For example, in this embodiment, there are two secondary shafts 423, two secondary wheels 427 and two transmission belts 428. The diameters of the main wheel 426 and the secondary wheel 427 can be different, so that the rotation speed of the main shaft 421 and the secondary shaft 423 are different (especially the rotation speed of the secondary shaft can be different from the rotation speed of the main shaft). The main shaft 421 can be arranged at the central axis of the enzymolysis tank 40, or the main shaft 421 can be at least relative to the secondary shaft 423 close to the central axis of the enzymolysis tank 40. Multiple secondary shafts 423 can be evenly distributed along the circumference of the enzymolysis tank 40. The differential stirring device 42 can form the rotation of the main shaft 421, the revolution of the secondary shaft 423 and the rotation of the secondary shaft 423 in the enzymolysis tank 40 when stirring the casein. Compared with the ordinary stirring device, it can effectively enhance the shear force on the casein, slow down the formation of casein micelles, and avoid or reduce the formation of large casein clumps in the enzymolysis tank 40. It can be understood that in a modified example, the motor 425 can also be connected to a secondary shaft 423. After the secondary shaft 423 is connected to the main shaft 421, the remaining secondary shafts 423 that are not directly connected to the motor 425 can be connected to the main shaft 421 or the secondary shaft 423 connected to the motor.

[0086] Preferably, the diameter of the main wheel 426 may be greater than the diameter of the secondary wheel 427. More preferably, the diameter of the main wheel 426 may be 1.5 to 2.5 times the diameter of the secondary wheel 427.

[0087] It can be understood that there is no special restriction on the blade arrangement form and blade type of the main shaft 421 and the secondary shaft 423, and they can be set according to the actual production needs. The main wheel 426 and the secondary wheel 427 can be pulleys, and the transmission belt 428 can be a transmission belt. The main wheel 426, the secondary wheel 427 and the transmission belt 428 can also be other synchronous pulley systems; or, the transmission belt 428 can also be replaced by a transmission chain, and the main wheel 426 and the secondary wheel 427 can also be sprockets, that is, the above-mentioned pulley system is replaced by a transmission sprocket system.

[0088] Preferably, the differential stirring device 42 may further include a scraper 429 connected to the main shaft 421. The scraper 429 may be close to (close to) the inner wall of the enzymolysis tank 40, and when the main shaft 421 rotates, the scraper 429 may move along the inner wall of the enzymolysis tank 40, that is, the scraper 429 may rotate (revolve) around the main shaft 421 to scrape the material on the inner wall of the enzymolysis tank 40. The scraper 429 may be a strip scraper, a sheet scraper, a cylindrical scraper, etc.

[0089] Further, such as Figure 4 and Figure 5 As shown, the enzymolysis tank 40 can be connected with the first external circulation pipeline 61. The two ends of the first external circulation pipeline 61 can be connected to the bottom and the top of the enzymolysis tank 40 respectively, so that the material in the enzymolysis tank 40 is extracted from the bottom and then passed from the top, so as to realize the circulation of the material in the enzymolysis tank 40 in the upper and lower directions of the enzymolysis tank and outside. The first external circulation pipeline 61 can be provided with a first circulation pump 71, and preferably, the first circulation pump 71 can be a centrifugal pump. It can be understood that the first external circulation pipeline 61 is provided to further enhance the fluidity of the material in the enzymolysis tank 40, and it can further slow down the formation of casein micelles in combination with the above-mentioned differential stirring device 42, avoid or reduce the formation of larger casein micelles in the enzymolysis tank 40, and can also increase the enzymolysis efficiency and reduce the amount of enzyme.

[0090] like Figure 5 , Figure 6 and Fig. 9As shown, the first external circulation pipeline 61 may be provided with a pipeline type extrusion impactor 611. The pipeline type extrusion impactor 611 may be provided in a horizontal pipeline (a horizontally arranged pipeline) of the first external circulation pipeline 61. The pipeline type extrusion impactor 611 may include a joint 6111, a reducer 6112, an extrusion head 6113 and a spoiler groove 6114. The joint 6111 may be provided at both ends of the pipeline type extrusion impactor 611, and is used to connect the pipeline type extrusion impactor 611 to the pipeline, and the joint 6111 may be a threaded joint. The reducer 6112 may be one or two (exemplarily, two reducers 6112 are included in this embodiment), and the reducer 6112 may be connected to the joint 6111 and provided on the inner side of the joint 6111 (a side close to the middle of the pipeline type extrusion impactor), and at least part of the pipe diameter may be variable (in particular, at least part of the pipe diameter may be uniformly variable) to change the pressure of the material flowing through the pipeline type extrusion impactor 611. The extrusion head 6113 can be arranged on the inner side of the reducer 6112 on the inlet side, and it has a pipe mouth with a smaller diameter, which can further extrude the material to break the casein micelles. The spoiler groove 6114 can be arranged in the middle of the pipeline extrusion impactor 611, and the spoiler groove 6114 can be formed with an irregular flow path (a flow path with a change in diameter) to further apply pressure to the material to break the casein micelles. It can be understood that the casein micelles in the material will be subjected to greater pressure and shear force when passing through the reducer 6112, the extrusion head 6113 and the spoiler groove 6114, which can break the casein micelles and reduce the problems of casein agglomeration, precipitation and wall adhesion.

[0091] It can be understood that, under the combined action of the differential stirring device 42 and the first external circulation pipeline 61, the material in the enzymolysis tank 40 can fully circulate, thereby increasing the probability of the enzyme and the substrate acting together and increasing the efficiency of protein hydrolysis. That is, under the same enzymolysis time and other conditions, the amount of enzyme required in the enzymolysis tank 40 provided in this embodiment is less.

[0092] like Figure 4 As shown, the enzymolysis tank 40 can also be connected to a second external circulation pipeline 62, and the second external circulation pipeline 62 can be provided with an external heat exchanger 50 and a second circulation pump 72. The external heat exchanger 50 can include a heating mode and a cooling mode, which are respectively used to heat up or cool down the material entering the second circulation pump 72. The second circulation pump 72 can be a centrifugal pump. It can be understood that multiple enzymolysis tanks 40 can share part of the second external circulation pipeline 62, including a shared part of the pipeline and a shared external heat exchanger 50. The two ends of the second external circulation pipeline 62 can be connected to the bottom and the upper part of the enzymolysis tank 40, respectively, so that the material in the enzymolysis tank 40 is extracted from the bottom of the enzymolysis tank 40, and after the temperature of the material is regulated by the external heat exchanger 50, it is passed into the enzymolysis tank 40 from the upper part of the enzymolysis tank 40. Exemplarily, as Figure 4As shown, three enzymolysis tanks 40 can be connected in parallel, and the three enzymolysis tanks 40 can be connected to a second external circulation pipeline 62. Preferably, the external heat exchanger 50 can be a tubular heat exchanger. It can be understood that the external heat exchanger 50 provided in the second external circulation pipeline 62 can jointly adjust the material temperature in the enzymolysis tank with the heat exchange jacket 41 of the enzymolysis tank 40, especially, when the enzymolysis stage ends and the enzyme inactivation stage is entered, the external heat exchanger 50 can quickly increase the material temperature in the enzymolysis tank 40 with the heat exchange jacket 41. By reducing the temperature rise transition time from the enzymolysis stage to the enzyme inactivation stage, the stability of the quality of the enzymolysis casein polypeptide product can be improved.

[0093] Preferably, Figure 5 and Figure 6 As shown, the acid-base preparation device 30 (diluted alkali tank 33 and diluted acid tank 34) is connected to the pipeline outlet of the enzymolysis tank 40 and a three-fluid atomizing nozzle 80 can be set. Fig.10 and Fig.11 As shown, the three-fluid atomizing nozzle 80 is a triple sleeve structure, which can include an air inner tube 81, an acid and alkali liquid pipe 82 and an air outer tube 83. The air inner tube 81 can be arranged at the innermost side (i.e. the radial inner side of the acid and alkali liquid pipe) of the three-fluid atomizing nozzle 80, and the air outer tube 83 can be arranged at the outermost side (i.e. the radial outer side of the acid and alkali liquid pipe) of the three-fluid atomizing nozzle 80, and both can be fed with compressed gas. The acid and alkali liquid pipe 82 can be arranged at the middle layer of the three-fluid atomizing nozzle 80 for feeding acid or alkali liquid. The end of the outlet of the air inner tube 81 can be provided with a swirl sheet 84 so that the air at the outlet of the air inner tube 81 forms a swirl. Under the joint action of the compressed gas of the air inner tube 81 and the air outer tube 83, the acid or alkali solution at the outlet of the acid-alkali solution pipe 82 can be quickly atomized into fine droplets and mixed with the material in the enzymolysis tank 40, and the distribution of the acid-alkali droplets is uniform, which can avoid the local pH of the material being too high or too low to denature the protein. It is understandable that the three-fluid atomizing nozzle 80 can be connected to a compressed gas pipeline or a compressed gas device. Here, the compressed gas can be compressed air (the oxygen in the air has almost no effect on the reaction in the enzymolysis tank), and the compressed gas can also be an inert gas such as nitrogen.

[0094] The preparation system may also include a cleaning in place unit (CIP) for cleaning the equipment and pipelines of the preparation system.

[0095] The embodiments of the present application also provide a control method for the above-mentioned enzymatic casein polypeptide industrial preparation system.

[0096] First, a casein solution with a solid content of 10%-20% can be selected and pasteurized by a sterilization device 10. The sterilization temperature can be 75-85°C (degrees Celsius), and the sterilization and heat preservation time can be 15-30s (seconds). The pasteurized material can be cooled to 4-6°C and passed into the enzymolysis tank 40 for temporary storage.

[0097] Accurately weigh the enzyme preparation and add it into the enzyme adding tank 20, and add RO water (reverse osmosis water) to fully dissolve the enzyme preparation.

[0098] Weigh the nitric acid solution and food-grade sodium hydroxide solid and add them into the concentrated acid tank 31 and the concentrated alkali tank 32 respectively. After stirring to fully dissolve, the acid solution and the alkali solution are respectively pumped into the dilute acid tank 34 and the dilute alkali tank 33 for accurate preparation.

[0099] The heat exchange jacket of the enzymolysis tank 40 is started to heat the material in the enzymolysis tank 40. The saturated steam pressure of the steam passed into the heat exchange jacket 41 can be 0.2-0.5Mpa (megapascals), and the saturated steam temperature can be 132-158°C. The material is heated from 4-6°C to 40-60°C, so that the material is at a suitable enzymolysis temperature, and the heating time can be less than or equal to 30 minutes.

[0100] During the above-mentioned heating process, the differential stirring device 42 can be started, the speed of the main shaft 421 of the differential stirring device 42 can be 10-30r / min (revolutions per minute), and the speed of the secondary shaft 423 can be 15-75r / min. At the same time, the first circulation pump 71 of the first external circulation pipeline 61 can be started to allow the material to continuously circulate through the first external circulation pipeline.

[0101] Set the pH control range of the enzymatic solution, open the compressed gas valve connected to the three-fluid atomizing nozzle 80, and introduce compressed gas into the air inner tube 81 and the air outer tube 83. The pressure of the compressed gas can be 0.1-0.6Mpa. The precisely prepared acid or alkali solution is introduced into the acid and alkali solution tube 82, so that the acid or alkali solution is quickly atomized into small droplets of 0.03-0.1μm (micrometer) under the action of the internal and external compressed gases and the swirl sheet 84 and evenly dispersed in the material.

[0102] During the enzymolysis process, the first circulation pump 71 is continuously turned on, and the material can be continuously circulated through the first external circulation pipeline 61. During the circulation process, the casein micelles can be broken by the material flowing through the pipeline extrusion impactor 611. The differential stirring device 42 of the enzymolysis tank 40 and the first external circulation pipeline 61 are operated simultaneously at least for part of the time, so that the material in the enzymolysis tank 40 can flow fully.

[0103] After the enzymolysis process is completed, the first circulation pump 71 is turned off, the steam pressure of the heat exchange jacket 41 is increased, and the second circulation pump 72 and the heating mode of the external heat exchanger 50 are started, so that the enzymolysis liquid in the enzymolysis tank 40 is heated by the steam of the heat exchange jacket 41 and the external heat exchanger 50 at the same time in a "double heating" mode. The material of 1000-5000kg (kilograms) can be heated from the enzymolysis temperature of 40-60℃ to the enzyme inactivation temperature of 85-95℃ within 2-6min. It can be understood that after the enzymolysis process is completed, if the enzyme is not inactivated, the polypeptides formed after the hydrolysis of casein may be further enzymolyzed, affecting the quality of the product.

[0104] After reaching the enzyme inactivation temperature, the second circulation pump 72 and the heating mode of the external heat exchanger 50 can be turned off, and the steam pressure of the heat exchange jacket 42 can be reduced, while the differential stirring device 42 can continue to operate.

[0105] After the enzyme inactivation process is completed, the second circulation pump 72 and the cooling mode of the external heat exchanger 50 can be started to cool the material to 4-6°C or 40-45°C according to the requirements of the subsequent production process, and then pumped into the storage tank for standby use.

[0106] Optionally, the method for regulating the pH value of the enzymatic hydrolyzate may be to continuously adjust the pH value to maintain a constant value during the enzymatic hydrolysis process; or to adjust the pH value once before the enzymatic hydrolysis is performed.

[0107] Optionally, in the enzyme inactivation process after the enzymatic hydrolysis process, if the material mass is within the range of 1000-2000 kg, only the heat exchange jacket 41 may be used for steam heating.

[0108] Three embodiments of specific parameter settings under the above control method are given below.

[0109] First embodiment

[0110] A casein solution with a solid content of 15% was selected, the sterilization temperature was 80°C, and the sterilization holding time was 24s;

[0111] During the enzymatic hydrolysis heating process, the saturated steam pressure of the heat exchange jacket 41 for heating the material is 0.3Mpa, the saturated steam temperature is 142°C, the material is heated from 4-6°C to 60°C enzymatic hydrolysis temperature, and the heating time is 27.3min;

[0112] The rotation speed of the main shaft 421 is 20r / min, and the rotation speed of the secondary shaft 423 is 40r / min;

[0113] Compressed gas pressure 0.3Mpa;

[0114] During the enzyme inactivation process, 3000 kg of material was heated from the enzymatic hydrolysis temperature of 60°C to the enzyme inactivation temperature of 90°C, which took 4.2 minutes.

[0115] Second embodiment

[0116] A casein solution with a solid content of 10% was selected, the sterilization temperature was 75°C, and the sterilization holding time was 30s;

[0117] During the enzymatic hydrolysis heating process, the saturated steam pressure of the heat exchange jacket 41 for heating the material is 0.2Mpa, the saturated steam temperature is 132°C, the material is heated from 4-6°C to 40°C enzymatic hydrolysis temperature, and the heating time is 24.6min;

[0118] The speed of the main shaft 421 is 10r / min, and the speed of the secondary shaft 423 is 15r / min;

[0119] Compressed gas pressure 0.1Mpa;

[0120] During the enzyme inactivation process, the temperature of 1000 kg of material was raised from the enzymatic hydrolysis temperature of 40°C to the enzyme inactivation temperature of 85°C, which took 2.3 minutes.

[0121] Third embodiment

[0122] A casein solution with a solid content of 20% was selected, the sterilization temperature was 85°C, and the sterilization holding time was 15s;

[0123] During the enzymatic hydrolysis heating process, the saturated steam pressure of the heat exchange jacket 41 for heating the material is 0.5 MPa, the saturated steam temperature is 158°C, the material is heated from 4-6°C to 60°C enzymatic hydrolysis temperature, and the heating time is 29.6 min;

[0124] The speed of the main shaft 421 is 30r / min, and the speed of the secondary shaft 423 is 75r / min;

[0125] Compressed gas pressure 0.6Mpa;

[0126] During the enzyme inactivation process, 5000 kg of material was heated from the enzymatic hydrolysis temperature of 60°C to the enzyme inactivation temperature of 95°C, which took 5.6 minutes.

[0127] It should be understood that some aspects of the above-mentioned embodiments may be appropriately combined.

[0128] The advantages of the above technical solution compared with the prior art are illustrated below with examples and comparative examples.

[0129] 1. Compare the material status during the enzymatic hydrolysis process and after the enzyme inactivation

[0130] Among them, comparative examples 1 and 2 are based on the first embodiment, with some preparation conditions changed, so that the test results of comparative examples 1 and 2 are compared with the test results of the first embodiment.

[0131] Table 1: Comparison of material states of each embodiment and comparative examples 1 and 2

[0132]

[0133] It can be seen from Table 1 above that the material state of each embodiment of the present application is better than the technical solution in the above comparative example.

[0134] 2. Compare the system heating efficiency

[0135] Table 2: Comparison of heating efficiency of the first embodiment and comparative examples 3, 4 and 5

[0136]

[0137] It can be seen from Table 2 above that the heating speed of the first embodiment of the present application is faster than that of the comparative example.

[0138] The following briefly describes some of the beneficial effects of the above-mentioned embodiments of the present application.

[0139] 1. The industrial preparation system of enzymatic casein polypeptide provided in this embodiment adopts a differential stirring device in the enzymatic hydrolysis tank, which greatly enhances the shear force on casein, slows down the formation of casein micelles, and eliminates or reduces the formation of larger casein micelles in the enzymatic hydrolysis tank.

[0140] 2. The industrial preparation system of enzymatic casein polypeptide provided in this embodiment adopts a pipeline extrusion impactor, which can be installed on the horizontal circulation pipeline of the material external circulation in the enzymatic hydrolysis process to fully break up the formed casein micelles.

[0141] 3. The pH adjustment of the industrial preparation system of enzymatic casein polypeptide provided in this embodiment is more gentle. Through the three-fluid atomizing nozzle, the acid solution and the alkali solution can be formed into small droplets of 0.05-0.1 μm, which are evenly spread in the liquid feed to avoid the local pH of the solution system being too high or too low, causing casein denaturation and precipitation.

[0142] 4. The enzymatic hydrolysis reaction efficiency of the industrial preparation system of enzymatic casein polypeptide provided in this embodiment is high. This technical solution adopts a dual circulation mode of stirring paddle disturbance in the tank and circulation outside the tank, so that the feed liquid can circulate fully, increase the probability of the enzyme and substrate to act, and improve the enzymatic hydrolysis efficiency. The amount of enzyme can be reduced under the same enzymatic hydrolysis time conditions.

[0143] 5. The industrial preparation system of enzymatic casein polypeptide provided in this embodiment has a fast heating rate, and adopts a "double heating" mode of heat exchange jacket steam heating and external heat exchanger heating. Under experimental conditions, it only takes 5-6 minutes to heat a single tank of 5000kg of material from the enzymatic hydrolysis temperature of 60°C to the enzyme inactivation temperature of 90°C, which greatly increases the batch-to-batch stability of mild and moderate enzymatic casein polypeptide products.

[0144] It is understood that in the present application, when the number of parts or components is not particularly limited, the number may be one or more, and the multiple here means two or more. For the case where the number of parts or components shown in the drawings and / or described in the specification is a specific number such as two, three, four, etc., the specific number is usually exemplary and not restrictive, and it can be understood as multiple, that is, two or more, but this does not mean that the present application excludes the case of one.

[0145] It should be understood that the above embodiments are merely exemplary and are not intended to limit the present application. Those skilled in the art may make various modifications and changes to the above embodiments under the guidance of the present application without departing from the scope of the present application.

Claims

1. An industrial preparation system for enzymatic casein polypeptide, characterized in that: The invention comprises one or more enzymolysis tanks (40) and one or more first external circulation pipelines (61), wherein the enzymolysis tank (40) comprises a heat exchange jacket (41) and a differential stirring device (42), wherein the heat exchange jacket (41) is arranged on the wall surface of the enzymolysis tank (40) and is used to control the temperature of the material in the enzymolysis tank (40). The differential stirring device (42) comprises a main shaft (421), a plurality of main propellers (422), a plurality of secondary shafts (423), a plurality of secondary propellers (424), and a motor (425). The plurality of main propellers (422) are connected to the main shaft (421), and the plurality of secondary propellers (424) are connected to the secondary shaft (423); the motor (425) is connected to the main shaft (421) or one of the secondary shafts (423), and at least one of the secondary shafts (423) is transmission-connected to the main shaft (421), so that one of the motors drives the main shaft and the secondary shaft (423) to rotate. The rotation speeds of the main shaft (421) and the secondary shaft (423) are different, so as to form a differential rotation to slow down the formation of casein micelles. The two ends of the first external circulation pipeline (61) are respectively connected to the bottom and the top of the enzymolysis tank (40). The first external circulation pipeline (61) is provided with a first circulation pump (71) so that the material in the enzymolysis tank (40) is drawn out from the bottom of the enzymolysis tank (40) and then passed into the enzymolysis tank (40) from the top of the enzymolysis tank (40), so that the material circulates in the upper and lower directions and outside the enzymolysis tank (40) to improve the enzymolysis efficiency. The first external circulation pipeline (61) is also provided with a pipeline-type extrusion impactor (611). The pipeline-type extrusion impactor (611) comprises a joint (6111), a reducer (6112), an extrusion head (6113) and a spoiler groove (6114). The joints (6111) are arranged at both ends of the pipeline-type extrusion impactor (611) to connect the pipeline, and the reducer (6112) is arranged on the inner side of the joint (6111). The diameter of at least a part of the reducer (6112) is variable. The extrusion head (6113) is arranged on the inner side of the reducer (6112) and has a pipe mouth capable of extruding materials. The spoiler groove (6114) is arranged in the middle of the pipeline-type extrusion impactor (611).

2. The industrial preparation system of enzymatic casein polypeptide according to claim 1, characterized in that: The motor (425) is connected to the main shaft (421), The secondary shaft (423) is connected to the primary shaft (421) via a connecting rod, so that the secondary shaft (423) can rotate around the primary shaft (421). The differential stirring device (42) comprises a main wheel (426), a secondary wheel (427) and a transmission belt (428) or a transmission chain. The main wheel (426) is connected to the main shaft (421), the secondary wheel (427) is connected to the secondary shaft (423), and the transmission belt (428) or the transmission chain is sleeved on the main wheel (426) and the secondary wheel (427), so that the secondary wheel (427) rotates with the rotation of the main wheel (426). The diameter of the main wheel (426) is greater than the diameter of the secondary wheel (427), so that the rotation speed of the main shaft (421) is lower than the rotation speed of the secondary shaft (423), and the main shaft (421) is arranged closer to the central axis of the enzymolysis tank (40) than the secondary shaft (423).

3. The industrial preparation system of enzymatic casein polypeptide according to claim 1, characterized in that: The differential stirring device (42) further comprises a scraper (429), wherein the scraper (429) is connected to the main shaft (421) and rotates with the main shaft (421), and the scraper (429) is close to the inner wall of the enzymolysis tank (40) to clean the material on the inner wall of the enzymolysis tank (40).

4. The industrial preparation system of enzymatic casein polypeptide according to claim 1, characterized in that: The system further comprises a second external circulation pipeline (62), wherein the second external circulation pipeline (62) is provided with a second circulation pump (72) and an external heat exchanger (50). The two ends of the second external circulation pipeline (62) are respectively connected to the bottom and the top of the enzymolysis tank (40), so that the material in the enzymolysis tank (40) is extracted from the bottom of the enzymolysis tank (40), and after the material temperature is regulated by the external heat exchanger (50), it is passed into the enzymolysis tank (40) from the top of the enzymolysis tank (40).

5. The industrial preparation system of enzymatic casein polypeptide according to claim 4, characterized in that: It comprises a plurality of the enzymolysis tanks (40) and a plurality of the first external circulation pipelines (61) of the same number, wherein each of the plurality of first external circulation pipelines (61) is connected to one of the enzymolysis tanks (40), The multiple enzymolysis tanks (40) are connected in parallel, and each of the multiple enzymolysis tanks (40) is connected to one second external circulation pipeline (62).

6. The industrial preparation system of enzymatic casein polypeptide according to claim 1, characterized in that: It also includes an acid-base preparation device (30), wherein the acid-base preparation device (30) is connected to the enzymolysis tank (40). The end of the pipeline connecting the acid-base preparation device (30) to the enzymolysis tank (40) is provided with a three-fluid atomizing nozzle (80). The three-fluid atomizing nozzle (80) comprises an inner air tube (81), an acid-base liquid tube (82) and an outer air tube (83), wherein the inner air tube (81) is located radially inside the acid-base liquid tube (82), the acid-base liquid tube (82) is located radially inside the outer air tube (83), and a swirl sheet (84) is provided at the end of the inner air tube (81) to form a gas swirl. The acid-alkali liquid pipe (82) can be fed with acid or alkali liquid, and the inner air pipe (81) and the outer air pipe (83) can be fed with air, so that the acid or alkali liquid can form atomized droplets under the action of the airflow.

7. The industrial preparation system of enzymatic casein polypeptide according to claim 1, characterized in that: It also includes a sterilization device (10) and one or more enzyme adding tanks (20), The sterilization device (10) is connected to the enzymolysis tank (40) to pass the sterilized casein solution into the enzymolysis tank (40). The enzyme addition tank (20) is connected to the enzymolysis tank (40) so as to introduce the enzyme preparation into the enzymolysis tank (40).

8. A control method for an industrial preparation system of enzymatic casein polypeptides, characterized in that: The industrial preparation system of enzymatic casein polypeptides according to any one of claims 1 to 7, During the enzymatic hydrolysis of casein, the differential stirring device (42) of the enzymatic hydrolysis tank (40) and the first external circulation pipeline (61) operate simultaneously for at least part of the time period, so that the material in the enzymatic hydrolysis tank (40) flows.

9. A control method for an industrial preparation system of enzymatic casein polypeptides, characterized in that: Applicable to the industrial preparation system of enzymatic casein polypeptide according to claim 4 or 5, During the process of inactivating the enzyme in enzymatic casein, the heat exchange jacket (41) of the enzymatic hydrolysis tank (40) and the second external circulation pipeline (62) operate simultaneously, so that the material in the enzymatic hydrolysis tank (40) is heated up.

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