Monopterus albus egg artificial fertilization device and fertilization method

Abstract

The invention discloses an artificial fertilization device and method for ricefield eel eggs. The artificial fertilization device comprises a base, a spray head driving assembly, an egg disc transmission assembly and an activation unit. The nozzle driving assembly is installed on the top of the base, the egg disc transmission assembly is located at the bottom of the nozzle driving assembly, and the activation unit comprises an activation liquid storage box, a conveying pump and a spraying pipe. According to the artificial fertilization method for the ricefield eel eggs, diluted semen of ricefield eels is evenly attached to the surfaces of egg membranes of the ricefield eel eggs in an atomization mode, fertilization is conducted on the ricefield eel eggs in a first attachment activation mode, and the wandering distance of the sperms of the ricefield eels after activation can be shortened to a great extent; by means of the fertilization device, finless eel sperms near the fertilization holes can enter the fertilization holes in a short time to be combined with ova to complete fertilization, finless eel roes cannot be touched in the whole process, no damage is caused to the roes, and the fertilization success rate of the roes is increased to a great extent.

Description

Technical Field

[0001] The invention relates to the technical field of agricultural product breeding, and in particular to an artificial fertilization device and a fertilization method for yellow eel eggs. Background Art

[0002] Artificial breeding of rice field eels is mainly divided into ecological breeding in paddy fields and fully artificial breeding indoors.

[0003] Among them, in the indoor fully artificial breeding technology, the eel egg fertilization method is mainly divided into wet fertilization and dry fertilization. The wet fertilization is to pour the taken out fish eggs and sperm into the water at the same time, and the dry fertilization is to mix the fish eggs and sperm first and then pour them into the water.

[0004] Rice field eel is a kind of fish with relatively small spawning volume. Each female carries between dozens and a thousand eggs. Dry fertilization takes a long time to operate, and the eggs will be damaged during mixing and stirring, which will affect the fertilization rate. Therefore, the wet fertilization operation is basically adopted in rice field eel farming at present.

[0005] However, wet fertilization actually simulates the natural fertilization process in a small space. However, since the survival time of eel sperm after being activated by water is usually less than five minutes, the optimal fertilization time is 1-2 minutes. In addition, eel eggs are large, sperm swims slowly, and an egg has only one fertilization hole. After the egg encounters water, it quickly absorbs water. After it is full of water, the fertilization hole is closed, and sperm cannot enter normally for fertilization. If the problem of low fertilization efficiency is solved by increasing the density of sperm, the demand for male broodstock will increase greatly, which is not conducive to the large-scale breeding of all artificial seedlings.

[0006] Based on the difficulties currently existing in the artificial breeding technology of the above-mentioned rice field eel, we propose an artificial fertilization device and fertilization method for rice field eel eggs. Summary of the invention

[0007] The purpose of the present invention is to provide an artificial fertilization device and a fertilization method for yellow eel eggs, so as to solve the above problems existing in the prior art.

[0008] In order to achieve the above object, the present invention adopts the following technical solutions:

[0009] An artificial fertilization device for yellow eel eggs comprises a base, a nozzle driving assembly, an egg tray transmission assembly and an activation unit.

[0010] Furthermore, the nozzle drive assembly is installed on the top of the base, and the nozzle drive assembly includes a vertical frame fixedly installed with the base, a No. 2 screw transmission mechanism is horizontally installed on the vertical frame, a carrier plate is installed at the bottom of the screw slider of the No. 2 screw transmission mechanism, an atomization device is installed at the end of the carrier plate, and the liquid inlet port of the atomization device is connected to the container tank through a pipeline, wherein the main function of the nozzle drive assembly is to drive the atomization device to move back and forth in a horizontal direction above the egg tray transmission assembly, and cooperate with the start-up of the atomization device to ensure that sperm is evenly attached to the egg membrane on the surface of the eel eggs.

[0011] Furthermore, the egg tray transmission assembly is arranged at the bottom of the nozzle driving assembly, and the egg tray transmission assembly also includes a machine base, a No. 1 screw transmission mechanism, a tray rack and an egg holding tray for holding eel eggs, the egg holding tray is placed on the tray rack, the sizes and structures between the tray rack and the egg holding tray are adapted to each other, and the tray rack is horizontally installed on the screw slider assembly of the No. 1 screw transmission mechanism; the activation unit includes an activation liquid storage tank, a delivery pump and a spray pipe; the liquid inlet end of the delivery pump is connected to the activation liquid storage tank through a pipeline, and the liquid outlet of the delivery pump is connected to the spray pipe.

[0012] Furthermore, the atomizing device is composed of a pump and an atomizing nozzle, wherein the atomizing nozzle is installed at the outlet end of the pump, the pipeline adopts a disposable sterile tube, and the two ends of the pipeline are connected to the container and the atomizing device by quick-release connectors.

[0013] Furthermore, a splash-proof enclosure is installed on the periphery of the base of the egg tray transmission assembly, and the bottom of the egg holding tray is designed to be a square flat-bottom structure, with both ends of the egg holding tray slidingly attached to the inner side of the splash-proof enclosure.

[0014] Furthermore, the activation liquid storage tank and the delivery pump are both installed on one side of the top of the base, and the spray pipe is transversely installed at the front end of the stand in the nozzle drive assembly.

[0015] Furthermore, a protective shell cover is installed on the top of the base, which wraps the nozzle drive assembly, the egg tray transmission assembly and the activation unit. An egg tray access port is opened at one end of the protective shell cover. The egg tray access port is mainly for the convenience of the operator to take and place the egg holding tray.

[0016] The invention discloses an artificial fertilization method for yellow eel eggs, comprising the following steps: step 1, preparing female and male broodstock, extracting yellow eel semen and female eel eggs, and placing the extracted yellow eel semen and eggs in sterile containers respectively for standby use.

[0017] Step 2: prepare the above-mentioned artificial insemination device for rice field eel eggs and fish egg hatching equipment. In order to provide a relatively sterile and safe environment for the fertilization of rice field eel eggs, it is necessary to implement all-round sterile disinfection inside and outside the equipment. The disinfection of the equipment can be selected but not limited to ozone disinfection.

[0018] Step 3: Add the eel semen and semen activation liquid into the corresponding storage tank and activation liquid storage box in the artificial insemination equipment prepared in step 2, in preparation for the eel egg fertilization operation. Before the eel semen is added into the storage tank, sperm preservation liquid needs to be added to dilute the eel sperm. The dilution concentration of the eel sperm is 900,000 to 1.1 million particles / ml.

[0019] Step 4: The fish eggs obtained in step 1 are contained in an egg containing dish, and the egg containing dish is placed on a tray rack in the equipment, the equipment is started, and a lead screw transmission mechanism in an egg tray transmission assembly is operated to move the tray rack together with the egg containing dish thereon to a nozzle driving assembly position; the atomizing device in the equipment is started to extract and atomize the yellow eel semen inside the containing tank so that it adheres to the surface of the yellow eel eggs contained in the egg containing dish;

[0020] Step 5: After all the eel eggs placed in the egg holding tray have completed the atomization attachment of the eel semen, the activation unit in the equipment is started, and the activation liquid in the activation liquid storage tank is transported to the spray pipe, sprayed into the egg holding tray, and the eel sperm attached to the surface of the eel eggs is activated to fertilize the eel eggs. When the eel eggs are fertilized, the ambient temperature is 21-28 degrees Celsius, and the fertilization time is 1-2 minutes;

[0021] Step 6: The fertilized yellow eel eggs can be placed in the fish egg hatching equipment prepared in step 2 to start hatching. After completing the fertilization operation of all yellow eel eggs, the equipment can be fully cleaned and disinfected again and sealed up for the next fertilization operation.

[0022] Beneficial effects:

[0023] The artificial insemination method for yellow eel eggs recorded in the present invention is to uniformly attach yellow eel sperm to the surface of the egg membrane of yellow eel eggs in an atomized manner, and then inject an activation liquid to activate the sperm so that the sperm fertilizes the egg. During the whole fertilization process, the yellow eel sperm is in direct contact with the egg before activation, which greatly shortens the migration distance of the yellow eel sperm after activation, so that the sperm can be combined with the egg in a short time to complete fertilization. In addition, the yellow eel eggs will not be touched during the whole process, and no damage will be caused to the fish eggs, thereby greatly improving the fertilization success rate of the fish eggs. BRIEF DESCRIPTION OF THE DRAWINGS

[0024] Figure 1 The internal structure of the artificial insemination device of the present invention is shown in FIG. Figure 1 ;

[0025] Figure 2 The internal structure of the artificial insemination device of the present invention is shown in FIG. Figure 2 ;

[0026] Figure 3 This is a schematic diagram of the appearance and structure of the artificial insemination device of the present invention;

[0027] Figure 4 It is a schematic diagram of the structure of the egg tray transmission assembly of the present invention;

[0028] Figure 5 It is a schematic diagram of the structure of the nozzle drive assembly of the present invention;

[0029] Figure 6 It is a schematic diagram of the activation unit structure of the present invention.

[0030] In the figure:

[0031] 1. Base;

[0032] 2. Nozzle drive assembly; 201. Stand; 202. No. 2 lead screw transmission mechanism; 203. Carrier plate; 204. Atomizing device; 205. Container; 206. Pipeline;

[0033] 3. Egg tray transmission assembly; 301. Machine base; 302. Splash-proof enclosure; 303. No. 1 lead screw transmission mechanism; 304. Tray rack; 305. Egg holding tray;

[0034] 4. Activation unit; 401. Activation liquid storage tank; 402. Delivery pump; 403. Spray pipe;

[0035] 5. Protective shell cover;

[0036] 6. Egg tray removal and placement. DETAILED DESCRIPTION

[0037] In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the present invention will be briefly introduced below in combination with the drawings and the description of the embodiments or the prior art. Obviously, the following description of the structure of the drawings is only some embodiments of the present invention. For ordinary technicians in this field, other drawings can be obtained based on these drawings without creative work. It should be noted that the description of these embodiments is used to help understand the present invention, but does not constitute a limitation of the present invention.

[0038] Example:

[0039] In view of the problems of long time consumption, many bad eggs and average fertilization rate in the dry and wet fertilization methods used in artificial insemination of eel seedlings in artificial breeding of eels, we propose an artificial insemination method for eel eggs and corresponding equipment that is completely different from the current traditional dry and wet fertilization operation steps and methods. The specific plan is as follows:

[0040] like Figure 1-2 As shown, this embodiment provides an artificial insemination device for yellow eel eggs, including a base 1, a nozzle driving assembly 2, an egg tray transmission assembly 3 and an activation unit 4.

[0041] like Figure 5 As shown, the nozzle drive assembly 2 is installed on the top of the base 1, and the nozzle drive assembly 2 includes a stand 201 fixedly installed with the base 1, and a No. 2 screw transmission mechanism 202 is horizontally installed on the stand 201, and a carrier plate 203 is installed at the bottom of the screw slider of the No. 2 screw transmission mechanism 202, and an atomizing device 204 is installed at the end of the carrier plate 203, and a liquid inlet port of the atomizing device 204 is connected to the containing tank 205 through a pipe 206, wherein the main function of the nozzle drive assembly 2 is to drive the atomizing device 204 to move back and forth in a horizontal direction above the egg tray transmission assembly 3, and cooperate with the start-up of the atomizing device 204 to ensure that sperm is evenly attached to the egg membrane on the surface of the eel eggs.

[0042] like Figure 1-4 As shown, in order to cooperate with the nozzle driving device and the atomizing device 204 installed thereon to realize the atomized attachment of the yellow eel sperm, and also to facilitate the subsequent taking and placing of the egg holding tray 305 containing yellow eel eggs, so as to facilitate the subsequent fertilization operation of the yellow eel eggs, the egg tray transmission component 3 is arranged at the bottom of the nozzle driving component 2, and the egg tray transmission component 3 also includes a base 301, a No. 1 screw transmission mechanism 303, a tray rack 304 and an egg holding tray 305 for containing yellow eel eggs. 05, the egg holding tray 305 is placed on the tray rack 304, the sizes and structures of the tray rack 304 and the egg holding tray 305 are adapted to each other, and the tray rack 304 is horizontally installed on the screw slider assembly of the No. 1 screw transmission mechanism 303; the activation unit 4 includes an activation liquid storage tank 401, a delivery pump 402 and a spray pipe 403; the liquid inlet end of the delivery pump 402 is connected to the activation liquid storage tank 401 through a pipeline, and the liquid outlet of the delivery pump 402 is connected to the spray pipe 403.

[0043] The atomizing device 204 is composed of a pump and an atomizing nozzle, wherein the atomizing nozzle is installed at the outlet end of the pump. The pipeline 206 adopts a disposable sterile tube, and the two ends of the pipeline 206 are connected to the container 205 and the atomizing device 204 by quick-release connectors. As we all know, the inner wall of the pipeline 206 is actually a narrow space, and it is difficult to clean and disinfect it thoroughly, and it is easy to breed bacteria. Therefore, this design can reduce the possibility of eel semen being contaminated by the external environment during transportation.

[0044] like Figure 4 As shown, in order to prevent a large amount of atomized liquid from overflowing outside the egg holding tray 305 when the diluted semen is atomized and attached, a splash-proof panel 302 is installed on the periphery of the base 301 of the egg tray transmission assembly 3, and in order to prevent the eel eggs from stacking and aggregating at the bottom of the tray, the bottom of the egg holding tray 305 is designed to be a square flat bottom structure, and the two ends of the egg holding tray 305 are slidably attached to the inner side of the splash-proof panel 302.

[0045] like Figure 1 , Figure 2 , Figure 6 As shown, in order to activate sperm in time after the sperm atomization and attachment of the eel eggs are completed, and to implement the final fertilization operation of the eel eggs, the activation liquid storage tank 401 and the delivery pump 402 are both installed on one side of the top of the base 1, and the spray pipe 403 is horizontally installed at the front end of the stand 201 in the nozzle drive assembly 2.

[0046] like Figure 3 As shown, in order to avoid the influence of the external environment or contamination of the eel sperm and eel eggs as much as possible, a protective shell cover 5 is installed on the top of the base 1 to wrap the nozzle drive assembly 2, the egg tray transmission assembly 3 and the activation unit 4. An egg tray access port 6 is opened at one end of the protective shell cover 5. The egg tray access port 6 is mainly for the convenience of the operator to take and place the egg holding tray 305.

[0047] The invention discloses an artificial fertilization method for yellow eel eggs, comprising the following steps: step 1, preparing female and male broodstock, extracting yellow eel semen and female eel eggs, and placing the extracted yellow eel semen and eggs in sterile containers respectively for standby use.

[0048] It needs to be further explained that in order to ensure the activity and quality of the extracted eel sperm, the weight of the male broodstock should be no less than 100 grams each, and when taking eggs from female broodstock, female broodstock with mature eggs should be selected.

[0049] Step 2: prepare the above-mentioned artificial insemination device for rice field eel eggs and fish egg hatching equipment. In order to provide a relatively sterile and safe environment for the fertilization of rice field eel eggs, it is necessary to implement all-round sterile disinfection inside and outside the equipment. The disinfection of the equipment can be selected but not limited to ozone disinfection.

[0050] Step 3: Add the eel semen and semen activation liquid into the corresponding container 205 and activation liquid storage box 401 in the artificial insemination equipment prepared in step 2, in preparation for the eel egg fertilization operation. Before the eel semen is added into the container 205, sperm preservation liquid needs to be added to dilute the eel sperm. The dilution concentration of the eel sperm is 900,000 to 1.1 million sperm / ml.

[0051] Step 4, the roe obtained in step 1 is contained in the ovum holding tray 305, and the ovum holding tray 305 is placed on the tray rack 304 in the equipment, the equipment is started, and No. 1 screw transmission mechanism 303 in the ovum tray transmission assembly 3 is operated, and the tray rack 304 is moved to the position of the spray head drive assembly 2 together with the ovum holding tray 305 thereon; the atomizing device 204 in the equipment is started to extract and atomize the yellow eel semen inside the holding tank 205 so that it is attached to the yellow eel ovum surface contained in the ovum holding tray 305;

[0052] Step 5: After all the eel eggs contained in the egg holding tray 305 have completed the atomization attachment of the eel semen, the activation unit 4 in the device is started, and the activation liquid in the activation liquid storage box 401 is transported to the spray pipe 403, sprayed into the egg holding tray 305, to activate the eel sperm attached to the surface of the eel eggs, and fertilize the eel eggs. When the eel eggs are fertilized, the ambient temperature is 21-28 degrees Celsius, and the fertilization time is 1-2 minutes;

[0053] Step 6: The fertilized yellow eel eggs can be placed in the fish egg hatching equipment prepared in step 2 to start hatching. After completing the fertilization operation of all yellow eel eggs, the equipment can be fully cleaned and disinfected again and sealed up for the next fertilization operation.

[0054] The difference between the eel egg fertilization method proposed by the present invention and the traditional dry fertilization method is that in the method recorded in the present invention, the eel semen (90-110 / ml) after atomization and dilution is used to make it adhere to the egg membrane surface of the fish eggs contained in the egg holding plate 305, which can realize the contact between the sperm and the fish eggs, and at the same time will not cause contact between the fish eggs, will not damage the egg membrane on the surface of the fish eggs, and can maximize the integrity of the fish eggs.

[0055] The difference between the present invention and the traditional wet fertilization method of eel eggs is that the fertilization method of eel eggs proposed in the present invention does not need to directly pour the eel semen and fish eggs into water or activation liquid at the same time, but through the means of atomization attachment, the dormant sperm can be evenly distributed on the egg membrane of the fish eggs, and then combined with the subsequent addition of activation liquid, the sperm is activated and then fertilized on the fish eggs. In this way, the migration distance of the eel sperm after activation can be greatly shortened, and the sperm can be combined with the egg in a short time to complete fertilization. Moreover, under the premise of the same fertilization rate, the fertilization method provided by the present invention has a sperm demand of about half of that of the traditional wet fertilization method.

[0056] Finally, it should be noted that the above description is only a preferred embodiment of the present invention and is not intended to limit the protection scope of the present invention. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention shall be included in the protection scope of the present invention.

Claims

1. An artificial insemination device for eel eggs, characterized in that: It comprises a base (1), a nozzle driving assembly (2), an egg tray transmission assembly (3) and an activation unit (4); The nozzle drive assembly (2) is mounted on the top of the base (1), and the nozzle drive assembly (2) comprises a stand (201) fixedly mounted on the base (1), a second screw transmission mechanism (202) is horizontally mounted on the stand (201), a carrier plate (203) is mounted at the bottom of the screw slider of the second screw transmission mechanism (202), an atomizing device (204) is mounted at the end of the carrier plate (203), and a liquid inlet port of the atomizing device (204) is connected to the container (205) through a pipe (206); The egg tray transmission assembly (3) is located at the bottom of the nozzle driving assembly (2), and the egg tray transmission assembly (3) comprises a machine base (301), a first screw transmission mechanism (303), a tray frame (304) and an egg holding tray (305); the egg holding tray (305) is placed on the tray frame (304), and the tray frame (304) is horizontally mounted on the screw slider assembly of the first screw transmission mechanism (303); The activation unit (4) comprises an activation liquid storage tank (401), a delivery pump (402) and a spray pipe (403); a liquid inlet end of the delivery pump (402) is connected to the activation liquid storage tank (401) via a pipeline, and a liquid outlet of the delivery pump (402) is connected to the spray pipe (403).

2. The artificial insemination device for yellow eel eggs according to claim 1, characterized in that: The atomizing device (204) is composed of a pump and an atomizing nozzle, the pipeline (206) is a disposable sterile tube, and the two ends of the pipeline (206) are connected to the container (205) and the atomizing device (204) by quick-release connectors.

3. The artificial insemination device for yellow eel eggs according to claim 1, characterized in that: The base (301) of the egg tray transmission assembly (3) is provided with a splash-proof enclosure (302) on its periphery, and the egg holding tray (305) is designed as a square flat bottom tray, with two ends of the egg holding tray (305) slidingly attached to the inner side of the splash-proof enclosure (302).

4. The artificial insemination device for yellow eel eggs according to claim 1, characterized in that: The activation liquid storage tank (401) and the delivery pump (402) are both installed on one side of the top of the base (1), and the spray pipe (403) is installed horizontally at the front end of the stand (201) in the nozzle drive assembly (2).

5. The artificial insemination device for yellow eel eggs according to claim 1, characterized in that: A protective shell cover (5) is installed on the top of the base (1) and encloses the nozzle drive assembly (2), the egg tray transmission assembly (3) and the activation unit (4). An egg tray access opening (6) is provided at one end of the protective shell cover (5).

6. A method for artificial insemination of eel eggs, characterized in that: include: Step 1, preparing female and male broodstock, and extracting yellow field eel semen and female eel eggs, and placing the extracted yellow field eel semen and eggs in sterile containers for standby use; Step 2: prepare the artificial fertilization device for eel eggs and the fish egg hatching equipment as described in any one of claims 1 to 5, and perform all-round sterilization inside and outside the equipment for standby use; Step 3, respectively adding the yellow eel semen and the semen activation liquid into the corresponding container (205) and the activation liquid storage box (401) in the artificial insemination equipment prepared in step 2, so as to prepare for the yellow eel egg fertilization operation; Step 4: The fish eggs obtained in step 1 are contained in an egg holding tray (305), and the egg holding tray (305) is placed on a tray rack (304) in the device, the device is started, and a lead screw transmission mechanism (303) in the egg tray transmission assembly (3) is operated, and the tray rack (304) and the egg holding tray (305) thereon are moved to the position of the nozzle drive assembly (2); the atomizing device (204) in the device is started to extract and atomize the yellow eel semen inside the holding tank (205) so that it adheres to the surface of the yellow eel eggs contained in the egg holding tray (305); Step 5: After all the eel eggs contained in the egg holding tray (305) have been atomized and attached with the eel sperm, the activation unit (4) in the device is started to transport the activation liquid in the activation liquid storage box (401) to the spray pipe (403) and spray it into the egg holding tray (305), thereby activating the eel sperm attached to the surface of the eel eggs and fertilizing the eel eggs; Step 6: The fertilized yellow eel eggs can be placed in the fish egg hatching equipment prepared in step 2 to start hatching. After completing the fertilization operation of all yellow eel eggs, the equipment can be fully cleaned and disinfected again and sealed up for the next fertilization operation.

7. The method for artificial insemination of eel eggs according to claim 6, characterized in that: In the step three, before the eel sperm is added into the containing tank (205), sperm preservation liquid needs to be added to dilute the eel sperm, and the dilution concentration of the eel sperm is 0.9-1.1 million sperm / ml.

8. The method for artificial insemination of rice field eel eggs according to claim 6, characterized in that: In the step 5, when the eel eggs are fertilized, the ambient temperature is 21-28 degrees Celsius and the fertilization time is 1-2 minutes.

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