An insecticide composition containing a dihydrazide compound and its application

Abstract

The present invention belongs to the field of pesticide insecticide technology and discloses an insecticidal composition containing a dihydrazide compound and its use. The insecticidal composition comprises active ingredient A and active ingredient B, wherein active ingredient A is isoflualanam, and active ingredient B is a dihydrazide compound, wherein the dihydrazide compound is any one of tebufenozide, methoxyfenozide, or chlorfenazolidone. The mass ratio of active ingredient A to active ingredient B is 1:36 to 45:1. The insecticidal composition of the present invention rationally combines insecticides with different mechanisms of action, so that the resulting composition has a beneficial effect on pest control, expands the insecticidal spectrum, improves the rapid action and long-term effect of pest control, and helps prevent or overcome the development of drug resistance.

Description

Technical Field

[0001] The invention belongs to the technical field of pesticide killing, and discloses an insecticidal composition containing a dihydrazide compound and application thereof. Background Art

[0002] Bisaccharides are ecdysone receptor agonists. According to the Insecticide Resistance Working Group (IRAC) classification, they are classified as insect growth regulators (IGRs) along with five other insecticide categories: juvenile hormone analogs, chitin synthesis inhibitors, dipteran molting disruptors, and acetyl-CoA carboxylase inhibitors. IGRs differ from traditional neurotropic insecticides in that they mimic the effects of ecdysone, prematurely initiating the molting stage in larvae, leading to cessation of feeding and ultimately death, thereby achieving their control objectives. Bisaccharides are increasingly becoming the preferred agents for integrated pest management due to their high efficacy, low toxicity, low residue levels, and safety for the environment and non-target organisms.

[0003] In recent years, the banning of highly toxic pesticides and the increasing incidence of insect resistance have significantly reduced or even rendered ineffective the effectiveness of conventional chemical agents against pests and diseases, making pest control increasingly difficult. Therefore, the search for novel, low-toxic, and highly effective pesticides and the management of insect resistance have become a hot topic in pesticide research and development. This invention rationally compounds a dihydrazide insecticide with isoflualanam to study the field efficacy of this combination against lepidopteran pests, providing a theoretical basis for the scientific and rational use of pesticides. Summary of the Invention

[0004] To address the above-mentioned problems in the prior art, the present invention provides an insecticide composition containing a dihydrazide compound and its application. The insecticide composition has excellent control effects on agricultural lepidopteran pests, reduces the dosage of pesticides used, and slows down the development of pest resistance.

[0005] In order to achieve the above object, the present invention adopts the following technical solution: an insecticidal composition containing a hydrazide compound, the insecticidal composition comprising an active ingredient A and an active ingredient B, the active ingredient A is isoflualanam, the active ingredient B is a dihydrazide compound, the dihydrazide compound is any one of tebufenozide, methoxyfenozide or chlorfenapyr, and the mass ratio of the active ingredient A to the active ingredient B is 1:36 to 45:1, or any value within the above numerical range.

[0006] Furthermore, the active ingredient B is tebufenozide, and the mass ratio of isoflualanam to tebufenozide is 1:25 to 35:1, or any value within the above numerical range;

[0007] The active ingredient B is methoxyfenozide, and the mass ratio of isoflualanam to methoxyfenozide is 1:25 to 30:1, or any value within the above numerical range;

[0008] The active ingredient B is chlorfenapyr, and the mass ratio of isoflualanam to chlorfenapyr is 1:30 to 20:1, or any value within the above numerical range.

[0009] Furthermore, the active ingredient B is tebufenozide, and the mass ratio of isoflualanam to tebufenozide is 1:25 to 22:1, or any value within the above numerical range;

[0010] The active ingredient B is methoxyfenozide, and the mass ratio of isoflualanam to methoxyfenozide is 1:15 to 30:1, or any value within the above numerical range;

[0011] The active ingredient B is chlorfenapyr, and the mass ratio of isoflualanam to chlorfenapyr is 1:22 to 20:1, or any value within the above numerical range.

[0012] Furthermore, the active ingredient B is tebufenozide, and the mass ratio of isoflualanam to tebufenozide is 1:20 to 18:1, or any value within the above numerical range;

[0013] The active ingredient B is methoxyfenozide, and the mass ratio of isoflualanam to methoxyfenozide is 1:15 to 20:1, or any value within the above numerical range;

[0014] The active ingredient B is chlorfenapyr, and the mass ratio of isoflualanam to chlorfenapyr is 1:14 to 15:1, or any value within the above numerical range.

[0015] Furthermore, based on 100 wt% of the total weight of the insecticide composition, the total weight of the active ingredient A and the active ingredient B accounts for 0.5% to 80% of the total weight of the insecticide composition, or any value within the above numerical range.

[0016] Furthermore, the insecticide composition contains, in addition to the active ingredients, auxiliary ingredients permitted in pesticides, and the auxiliary ingredients are selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoaming agents, solvents, preservatives, stabilizers, synergists or carriers.

[0017] Furthermore, the wetting agent is selected from one or more of alkylbenzene sulfonate, alkylnaphthalene sulfonate, lignin sulfonate, sodium lauryl sulfate, sodium dioctyl sulfosuccinate, α-olefin sulfonate, alkylphenol polyoxyethylene ether, castor oil polyoxyethylene ether, alkylphenol ethoxylate, fatty alcohol ethoxylate, fatty alcohol polyoxyethylene ether sodium sulfate, silkworm feces, soapberry powder, soapberry powder, SOPA, detergent, emulsifier 2000 series and wetting penetrant F; and / or

[0018] The dispersant is selected from one or more of lignin sulfonate, alkylnaphthalene sulfonate formaldehyde condensate, naphthalene sulfonate, tristyrylphenol ethoxylate phosphate, fatty alcohol ethoxylate, alkylphenol polyoxyethylene ether, alkylphenol polyoxyethylene ether methyl ether condensate sulfate, fatty amine polyoxyethylene ether, glycerol fatty acid ester polyoxyethylene ether, polycarboxylates, polyacrylic acids, phosphates, EO-PO block copolymers and EO-PO graft copolymers; and / or

[0019] The emulsifier is selected from one or more of calcium dodecylbenzenesulfonate, alkylphenol formaldehyde resin polyoxyethylene ether, phenylethylphenol polyoxyethylene polyoxypropylene ether, fatty alcohol ethylene oxide-propylene oxide copolymer, styrylphenol polyoxyethylene ether, castor oil polyoxyethylene ether and alkylphenol ether phosphate; and / or

[0020] The thickener is selected from one or more of xanthan gum, organobentonite, gum arabic, sodium alginate, magnesium aluminum silicate, carboxymethyl cellulose and white carbon black; and / or

[0021] The disintegrant is selected from one or more of sodium sulfate, ammonium sulfate, aluminum chloride, sodium chloride, ammonium chloride, bentonite, glucose, sucrose, starch, cellulose, urea, sodium carbonate, sodium bicarbonate, citric acid and tartaric acid; and / or

[0022] The antifreeze agent is selected from one or more of alcohols, alcohol ethers, chlorinated hydrocarbons and inorganic salts; and / or

[0023] Defoaming agent selected from C 10 -C 20 Saturated fatty acid compounds, silicone oil, silicone compounds, C8-C 10 One or more of fatty alcohols; and / or

[0024] The solvent is selected from one or more of benzene, toluene, xylene, durene, methanol, ethanol, isopropanol, n-butanol, dimethyl sulfoxide, dimethylformamide, cyclohexanone, alkylene carbonate, diesel, solvent oil, vegetable oil, vegetable oil derivatives and water; and / or

[0025] The preservative is selected from one or more of propionic acid, sodium propionic acid, sorbic acid, sodium sorbic acid, potassium sorbic acid, benzoic acid, sodium benzoic acid, sodium p-hydroxybenzoic acid, methyl p-hydroxybenzoate, kasone and 1,2-benzisothiazolin-3-one; and / or

[0026] The stabilizer is selected from one or more of disodium hydrogen phosphate, oxalic acid, succinic acid, adipic acid, borax, 2,6-di-tert-butyl-p-cresol, triethanolamine oleate, epoxidized vegetable oil, kaolin, bentonite, attapulgite, white carbon black, talc, montmorillonite and starch; and / or

[0027] Synergists are selected from synergist, piperonyl butoxide; and / or

[0028] The carrier is selected from one or more of ammonium salts, ground natural minerals, ground artificial minerals, silicates, resins, waxes, solid fertilizers, water, organic solvents, mineral oils, vegetable oils and vegetable oil derivatives;

[0029] Furthermore, the insecticide composition is prepared into a formulation permitted by pesticides, and the formulation is a solid formulation or a liquid formulation;

[0030] Furthermore, the solid preparation includes powders, granules, pellets, tablets, strips, wettable powders, oil-dispersible powders, emulsion powders, water-dispersible granules, emulsion granules, water-dispersible tablets, soluble powders, soluble tablets or soluble granules;

[0031] The liquid preparations include soluble solutions, soluble gels, oils, film-spreading oils, emulsifiable concentrates, latexes, dispersible solutions, ointments, aqueous emulsions, oil emulsions, microemulsions, lipid suspensions, microcapsule suspensions, oil suspensions, dispersible oil suspensions, suspoemulsions, microcapsule suspension-suspension concentrates, microcapsule suspension-water emulsions, or microcapsule suspension-suspoemulsions;

[0032] Furthermore, the solid preparation is a water-dispersible granule or a wettable powder, and the liquid preparation is a suspension, emulsifiable concentrate, water emulsion, microemulsion or dispersible oil suspension.

[0033] The present invention also discloses the use of the above-mentioned insecticide composition and its preparation for preventing and controlling agricultural pests.

[0034] Furthermore, the pests are Lepidoptera pests;

[0035] Furthermore, the Lepidoptera includes, but is not limited to, Plutellaxylostella, diamondback moth, Polychrosis viteana (grapeberry moth), Prays endocarpa, Prays oleae (olive moth), Pseudaletia spp. (snoctuids), Pseudaletia unipunctata (armyworm), Pseudoplusia includes (soybean looper), Rachiplusia nu, Scirpophaga incertulas, Sesamia spp. (stemborers), Sesamia inferens (pink rice stem borer), Sesamia nonagrioides), Setora nitens, Sitotroga cerealella (Angoumois grain moth), Sparganothis pilleriana, Spodoptera spp. (snooper moths), Spodoptera exigua (beet armyworm), Spodoptera fugiperda (fall armyworm), Adoxophyes spp., Adoxophyes orana, Agrotis spp.(cutworm), Agrotis ipsilon (black cutworm), Alabama argillacea, Amorbia cuneana, Amyelosis transitella (navel orangeworm), Anarsia lineatella (peach twig borer), Anomissa bulifera (jute looper), Anticarsia gemma talis (velvet bean pelleter), Archips argyrospila (fruittree leafroller), Archips rosana (rose leaf roller), Argyrotaenia spp. (tortricidmoths), Argyrotaenia citrana, Autographa gamma, Bonagota cranaodes, Borbo cinnara), Caloptilia spp. (leaf miners), Capuareticulana, Carposina niponensis (peach fruit moth), Chilospp., Chlumetia transversa (mango shoot borer), Choristoneura rosaceana (obliquebanded leafroller), Chrysodeixis spp., Cnaphalocerus medinalis (grass leafroller), Colias spp., Conpomorpha cramerella, Cossus cossus, Crambus spp.) (Sod webworms), Cydia funebrana (plum fruit moth), Cydia molesta (oriental fruit moth), Cydia nignicana (pea moth), Cydia pomonella, Darna diducta, Diaphania spp. (stem borers), Diatraea spp. (stalk borers), Diatraea saccharalis (sugarcane borer), Diatraea graniosella (southwester cornborer), Earias spp. (cotton bollworms), Earias spp. (Egyptian bollworms). insulata (Egyptian bollworm), Earias vitella (rough northern bollworm), Ecdytopophaaurantianum, Elasmopalpus lignosellus (lesser cornstalk borer), Ephestia spp. (flour moths), Ephestia cautella (almond moth), Ephestia elutella (tobbaco moth), Ephestia kuehniella (Mediterranean flour moth), Epimeces spp., Epinotia aporema, Erionota thrax (banana skipper), Eupoecilia ambiguella, Euxoa auxiliaris (army cutworm), Feltia spp., Gortyna spp.(stemborers), Grapholita molesta (oriental fruit moth), Hedylepta indicate, bean leaf webber, Helicoverpas pp. (spotworms), Helicoverpa paarmigera, Helicoverpa zea, Heliothis spp. (spotworms), Heliothis virescens, tobacco budworm, Hellula undalis (cabbage webworm), Indarbela spp., root borers, Keiferia lycopersicella, (tomato pinworm), Leucinodes orbonalis (eggplant fruit borer). borer), Leucoptera malifoliella, Lithocollectis spp., Lobesia botrana (grape fruit moth), Loxagrotis spp. (southworm), Loxagrotis albicosta (western bean cutworm), Lymantria dispar (gypsy moth), Lyonetia clerkella (apple leaf miner), Mahasena corbetti (oil palm bagworm), Malacosoma spp.(tent caterpillars), Mamestra brassicae (cabbage armyworm), Maruca testulalis, Metisa plana, Mythimna unipuncta (true armyworm), Neoleucinodes elegantalis (small tomato borer), Operophthera brumata (winter moth), Ostrinia nubilalis (European corn borer), Pandemis cerasana, common currant tortrix, Pandemis heparana (brown apple tortrix), Papilio demodocus, Pectinophora gossypiella (pink bollworm), Peridroma species ( spp. (cutworm), Peridroma saucia (variegated cutworm), Perileucoptera coffeella (white coffee leafminer), Phthorimaea operculella (potato tuber moth), Phyllocnisitis citrella, Phyllonorycter spp. (leafminers), Pieris rapae (imported cabbageworm), Plathypena scabra, Plodia interpunctella (Indian mealmoth), Spodoptera oridania (southern armyworm), Synanthedon spp.) (root borers), Thecla basilides, Thermisiagemmatalis, Tineola bisselliella (webbing clothes moth), Trichoplusia ni (rape worm), Tuta absoluta, Yponomeuta spp., Zeuzera coffeae (red branch borer), and Zeuzera pyrina (leopard moth).

[0036] In particular, the insecticide composition of the present invention has excellent control effects on diamondback moth, beet armyworm and rice borer.

[0037] Furthermore, the insecticide composition or its formulation is applied to the pests to be controlled or the medium in which they grow.

[0038] In order to obtain the desired insecticidal effect, the dosage of the insecticidal composition varies depending on various factors, such as the crop to be protected, the type of pest, the degree of infection, climatic conditions, application site, application method, dosage form used, etc.

[0039] The beneficial effects of the present invention are as follows:

[0040] The insecticide composition of the present invention is compounded with isoflualanam and a dihydrazide compound having different action mechanisms. Within a suitable ratio, the composition has a significant synergistic effect on lepidopteran pests and a remarkable control effect. The composition reduces the dosage of the pesticide and slows down the development of pest resistance. DETAILED DESCRIPTION

[0041] In order to make the technical solutions, objectives and advantages of the present invention more clearly understood, the present invention is described with reference to the following specific embodiments. However, the present invention can be implemented in various forms and should not be limited to the embodiments described herein.

[0042] Preparation example:

[0043] Preparation Example 1: 20% isoflualanam·tebufenozide suspension (2:3)

[0044] Formula: 8% isoflualanam, 12% tebufenozide, 1% sodium lauryl sulfate, 2% sodium alkyl polyoxyethylene ether sulfonate, 3% polyoxyethylene sorbitan monooleate, 3% tristyrylphenol ethoxylate phosphate, 0.25% xanthan gum, 1% magnesium aluminum silicate, 4% propylene glycol, 0.1% sodium benzoate, 0.5% silicone oil, and deionized water to make up the balance;

[0045] Preparation method: According to the formula ratio, the active ingredients, surfactants and other functional additives are placed in a reactor in sequence, water is added and mixed evenly, and the suspension product is obtained by high-speed shearing, wet sand grinding, and finally homogenization filtration.

[0046] Preparation Example 2: 6% isoflualanam·tebufenozide emulsifiable concentrate (5:1)

[0047] Formula: 5% isoflualanam, 1% tebufenozide, 10% dimethyl sulfoxide, 10% tristyrylphenol polyoxyethylene ether, 2% calcium dodecylbenzenesulfonate, 25% propylene carbonate, and xylene to make up the balance;

[0048] Preparation method: add the measured active ingredients, solvent and cosolvent into a mixing kettle and stir to dissolve them, then add emulsifier, make up the balance with the remaining solvent, stir evenly in a stirring kettle, and filter to obtain the desired emulsifiable concentrate of the present invention.

[0049] Preparation Example 3: 30% isoflualanam·tebufenozide wettable powder (1:5)

[0050] Formula: 5% isoflualanam, 25% tebufenozide, 2% sodium lauryl sulfate, 3% sodium lignin sulfonate, 4% alkylnaphthalene sulfonate, 6% naphthalene sulfonate formaldehyde condensate, 15% kaolin, 10% white carbon black, and bentonite to make up the balance;

[0051] Preparation method: The active ingredients, dispersant, wetting agent and filler are mixed according to the formula ratio, stirred evenly in a stirring kettle, and pulverized and mixed evenly for multiple times in a jet mill to prepare the wettable powder of the composition of the present invention.

[0052] Preparation Example 4: 20% isoflualanam·methoxyfenozide suspension (4:1)

[0053] Formula: 16% isoflualanam, 4% methoxyfenozide, 1% triphenylethylphenol polyoxyethylene ether, 5% arylphenol polyoxyethylene ether phosphate, 1% naphthalenesulfonate formaldehyde condensate, 0.25% xanthan gum, 5% ethylene glycol, 0.1% potassium benzoate, 0.5% silicone oil, and deionized water to make up the balance;

[0054] Preparation method: Same as Preparation Example 1.

[0055] Preparation Example 5: 3% isoflualanam·methoxyfenozide emulsifiable concentrate (2:1)

[0056] Formula: 2% isoflualanam, 1% methoxyfenozide, 22% propylene glycol methyl ether, 12% ethylene glycol oxyethylene polyoxypropylene ether, 2% calcium dodecylbenzenesulfonate, 12% DMF, and methyl oleate makes up the balance.

[0057] Preparation method: same as Preparation Example 2.

[0058] Preparation Example 6: 36% isoflualanam·methoxyfenozide wettable powder (1:8)

[0059] Formula: 4% isoflualanam, 32% methoxyfenozide, 5% sodium salt of polycarboxylate, 2% sodium alkyl polyoxyethylene ether sulfonate, 2% BX powder, 7% white carbon black, and kaolin to make up the balance;

[0060] Preparation method: Same as Preparation Example 3.

[0061] Preparation Example 7: 16% isoflualanam·chlorfenapyr suspension (7:1)

[0062] Formula: 14% isoflualanam, 2% chlorfenapyr, 2% anhydrous sorbitan polyoxyethylene ether, 3% isotridecyl polyoxyethylene ether, 2% styrenated phenol polyoxyethylene ether phosphate, 2% sodium polycarboxylate, 1.5% magnesium aluminum silicate, 0.15% carboxyethyl cellulose, 1% sodium sorbate, 5% ethylene glycol, 0.5% silicone oil, and deionized water to make up the balance;

[0063] Preparation method: Same as Preparation Example 1.

[0064] Preparation Example 8: 9% isoflualanam·chlorfenapyr emulsifiable concentrate (5:4)

[0065] Formula: 5% isoflualanam, 4% chlorfenapyr, 12% EO / PO block copolymer, 12% acetophenone, 10% propylene glycol methyl ether, 3% calcium dodecylbenzenesulfonate, and trimethylbenzene to make up the balance;

[0066] Preparation method: Same as Preparation Example 2.

[0067] Preparation Example 9: 36% isoflualanam·chlorfenapyr wettable powder (2:7)

[0068] Formula: 8% isoflualanam, 28% chlorfenapyr, 2% sodium lauryl sulfate, 2% naphthalenesulfonate formaldehyde condensate, 5% alkylnaphthalenesulfonate, 5% fatty alcohol polyoxyethylene ether sulfate, 10% kaolin, 8% white carbon black, and bentonite to make up the balance;

[0069] Preparation method: Same as Preparation Example 3.

[0070] Example 1: Indoor bioactivity assay against Spodoptera exigua

[0071] Test basis: The test refers to NY / T 1154.14-2008 "Guidelines for Indoor Bioassay Tests of Pesticides - Insecticides Part 14: Leaf Dipping Method".

[0072] Test target: 3rd instar larvae of beet armyworm.

[0073] Test agents: isoflualanam, tebufenozide, methoxyfenozide, and chlorfenazolidone technical drugs.

[0074] Test method: Select sensitive test insects that are reared indoors and of the same age. Dissolve the above original drugs with a suitable solvent and then dilute with an aqueous solution containing 0.1% Tween-80. According to the activity of the drug, set up 5 mass concentration gradients according to the proportional method. Select fresh cabbage leaves that have not been treated with drugs and make leaf discs with a hole puncher. After dipping the leaf disc into the test solution for 10 seconds, take it out and dry it. Place it in a culture dish containing moisturizing filter paper and inoculate the test insects. The treated test insects are raised under the conditions of temperature (26±1)℃, relative humidity 65%±5%, and light cycle L:D=14h:10h. Each treatment is repeated 4 times, and 20 insects are immersed in each repetition. The corresponding organic solvent treatment without drugs is set as a blank control.

[0075] Experimental investigation: 72 hours after treatment, investigate the mortality of test insects and record the total number of insects and the number of dead insects.

[0076] Calculation method:

[0077] Based on the survey data, calculate the mortality rate of each treatment. Calculate as follows:

[0078]

[0079] Where:

[0080] P——mortality rate, in percentage (%);

[0081] K——indicates the number of dead insects, the unit is head;

[0082] N——represents the total number of insects processed, in heads.

[0083]

[0084] Where:

[0085] P1——adjusted mortality rate, in percentage (%);

[0086] P t ——Treatment mortality rate, expressed in percentage (%);

[0087] P0 - blank control mortality rate, in percentage (%).

[0088] If the control mortality rate is less than 5%, no correction is required; if the control mortality rate is between 5% and 20%, correction should be made according to the correction mortality formula; if the control mortality rate is greater than 20%, the test needs to be repeated.

[0089] The DPS statistical analysis system was used to analyze the toxicity regression equation, correlation coefficient and LC 50 The activity of the test agent on the biological test material is evaluated by the value.

[0090] The co-toxicity coefficient (CTC value) of the mixture is calculated as follows:

[0091]

[0092] Where:

[0093] ATI - measured toxicity index of mixture;

[0094] S——LC of standard pesticide 50 , the unit is milligrams per liter (mg / L);

[0095] M——LC of the mixture 50 , the unit is milligrams per liter (mg / L).

[0096] TTI=TI A ×P A +TI B ×P B

[0097] Where:

[0098] TTI – Theoretical Toxicity Index of Mixtures;

[0099] TI A ——Agent toxicity index;

[0100] P A ——The percentage of agent A in the mixture, in percentage (%);

[0101] TI B ——Toxicity index of agent B;

[0102] P B——The percentage of agent B in the mixture, in percentage (%).

[0103]

[0104] Where:

[0105] CTC – Co-toxicity coefficient;

[0106] ATI - measured toxicity index of mixture;

[0107] TTI - Theoretical Toxicity Index of Mixture.

[0108] A co-toxicity coefficient (CTC) of 120 or higher indicates a synergistic effect; a co-toxicity coefficient (CTC) of 80 or lower indicates an antagonistic effect; and a co-toxicity coefficient (CTC) of 80 or lower indicates an additive effect.

[0109] The indoor test results are shown in the table below:

[0110] Table 1 Results of indoor biological activity test of isoflualanam and tebufenozide against Spodoptera exigua

[0111]

[0112]

[0113] Laboratory test results showed that combining isoflualanam and tebufenozide at appropriate mass ratios exhibited a synergistic effect against Spodoptera exigua within a certain ratio range. When the mass ratio of isoflualanam to tebufenozide was 1:25 to 22:1, the co-toxicity coefficient against Spodoptera exigua was greater than 120, indicating a synergistic effect. When the mass ratio of isoflualanam to tebufenozide was 1:20 to 18:1, the co-toxicity coefficient against Spodoptera exigua was greater than 130, demonstrating a significant synergistic effect. When the mass ratio of isoflualanam to tebufenozide was 1:10 to 12:1, the co-toxicity coefficient exceeded 140, indicating a significant synergistic effect.

[0114] Table 2 Results of indoor biological activity test of isoflualanam and methoxyfenozide against Spodoptera exigua

[0115]

[0116] Laboratory test results showed that combining isoflualanam and methoxyfenozide in an appropriate mass ratio exhibited a synergistic effect against Spodoptera exigua. When the mass ratio of isoflualanam to methoxyfenozide was 1:25-30:1, the co-toxicity coefficient against Spodoptera exigua was greater than 120, indicating a synergistic effect. When the mass ratio of isoflualanam to methoxyfenozide was 1:15-30:1, the co-toxicity coefficient against Spodoptera exigua was greater than 130, indicating a significant synergistic effect. When the mass ratio of isoflualanam to methoxyfenozide was 1:15-20:1, the co-toxicity coefficient against Spodoptera exigua was greater than 140, indicating a significant synergistic effect.

[0117] Table 3 Results of indoor biological activity test of isoflualanam and chlorfenapyr against Spodoptera exigua

[0118]

[0119]

[0120] Laboratory test results showed that combining isoflualanam with chlorfenapyr in an appropriate mass ratio exhibited a synergistic effect against Spodoptera exigua. When the mass ratio of isoflualanam to chlorfenapyr was 1:30 to 20:1, the co-toxicity coefficient against Spodoptera exigua was greater than 120, indicating a synergistic effect. When the mass ratio of isoflualanam to chlorfenapyr was 1:22 to 15:1, the co-toxicity coefficient against Spodoptera exigua was greater than 140, demonstrating a significant synergistic effect.

[0121] Example 2: Indoor bioactivity assay test on rice stem borer

[0122] Test basis: The test refers to NY / T 1154.14-2008 "Guidelines for Indoor Bioassay Tests of Pesticides - Insecticides Part 14: Leaf Dipping Method".

[0123] Test target: 3rd instar larvae of the rice stem borer.

[0124] Test agents: isoflualanam, tebufenozide, methoxyfenozide, and chlorfenazolidone technical drugs.

[0125] Test Method: Select sensitive test insects of uniform age reared indoors. Dissolve the above active ingredients in a suitable solvent and dilute with an aqueous solution containing 0.1% Tween-80. Five concentration gradients were set according to the active ingredient activity using the equipotent method. Approximately 10 cm of the upper leaves of fresh, untreated rice plants in the peak tillering stage were immersed in the solution for 30 seconds. After air-drying on filter paper, approximately 20 rice leaves were spread flat in a 20 cm germination box. Third-instar larvae of the rice leaf roller were gently transferred to each box using a soft brush. Twenty test insects were placed in each box. The boxes were covered with plastic wrap and punctured with toothpicks for ventilation. The treated insects were housed at a temperature of (26 ± 1)°C, a relative humidity of 65% ± 5%, and a photoperiod of L:D = 14h:10h. Each treatment was replicated four times, with 20 insects immersed in each replicate. A blank control was also included using the corresponding organic solvent without the agent. After 48 hours, the mortality of the larvae was observed. No reaction to the needle prick or the larvae's body color became black and shrunken was considered the mortality standard of the rice leaf roller larvae.

[0126] Calculation method:

[0127] Based on the survey data, calculate the mortality rate of each treatment. Calculate as follows:

[0128]

[0129] Where:

[0130] P——mortality rate, in percentage (%);

[0131] K——indicates the number of dead insects, the unit is head;

[0132] N——represents the total number of insects processed, in heads.

[0133]

[0134] Where:

[0135] P1——adjusted mortality rate, in percentage (%);

[0136] P t ——Treatment mortality rate, expressed in percentage (%);

[0137] P0 - blank control mortality rate, in percentage (%).

[0138] If the control mortality rate is less than 5%, no correction is required; if the control mortality rate is between 5% and 20%, correction should be made according to the correction mortality formula; if the control mortality rate is greater than 20%, the test needs to be repeated.

[0139] The DPS statistical analysis system was used to analyze the toxicity regression equation, correlation coefficient and LC 50The activity of the test agent on the biological test material is evaluated by the value.

[0140] The co-toxicity coefficient (CTC value) of the mixture is calculated as follows:

[0141]

[0142] Where:

[0143] ATI - measured toxicity index of mixture;

[0144] S——LC of standard pesticide 50 , the unit is milligrams per liter (mg / L);

[0145] M——LC of the mixture 50 , the unit is milligrams per liter (mg / L).

[0146] TTI=TI A ×P A +TI B ×P B

[0147] Where:

[0148] TTI – Theoretical Toxicity Index of Mixtures;

[0149] TI A ——Agent toxicity index;

[0150] P A ——The percentage of agent A in the mixture, in percentage (%);

[0151] TI B ——Toxicity index of agent B;

[0152] P B ——The percentage of agent B in the mixture, in percentage (%).

[0153]

[0154] Where:

[0155] CTC – Co-toxicity coefficient;

[0156] ATI - measured toxicity index of mixture;

[0157] TTI - Theoretical Toxicity Index of Mixture.

[0158] A co-toxicity coefficient (CTC) of 120 or higher indicates a synergistic effect; a co-toxicity coefficient (CTC) of 80 or lower indicates an antagonistic effect; and a co-toxicity coefficient (CTC) of 80 or lower indicates an additive effect.

[0159] The indoor test results are shown in the table below:

[0160] Table 4 Results of indoor bioactivity test on isoflualanam and tebufenozide against rice stem borer

[0161]

[0162]

[0163] Laboratory test results showed that combining isoflualanam and tebufenozide at appropriate mass ratios exhibited a synergistic effect against the rice stem borer within a certain ratio range. When the mass ratio of isoflualanam to tebufenozide was 1:25-35:1, the co-toxicity coefficient against the rice stem borer exceeded 120, indicating a synergistic effect. When the mass ratio of isoflualanam to tebufenozide was 1:18-24:1, the co-toxicity coefficient against the rice stem borer exceeded 130, indicating a significant synergistic effect. When the mass ratio of isoflualanam to tebufenozide was 1:18-16:1, the co-toxicity coefficient exceeded 140, indicating a significant synergistic effect.

[0164] Table 5 Results of indoor biological activity test on isoflualanam and methoxyfenozide against rice stem borer

[0165]

[0166] Laboratory test results showed that combining isoflualanam and methoxyfenozide in an appropriate mass ratio exhibited a synergistic effect against the rice stem borer. When the mass ratio of isoflualanam to methoxyfenozide was 1:24 to 30:1, the co-toxicity coefficient against the rice stem borer was greater than 120, indicating a synergistic effect. When the mass ratio of isoflualanam to methoxyfenozide was 1:16 to 16:1, the co-toxicity coefficient against the rice stem borer was greater than 130, indicating a significant synergistic effect. When the mass ratio of isoflualanam to methoxyfenozide was 1:8 to 16:1, the co-toxicity coefficient against the rice stem borer was greater than 140, indicating a significant synergistic effect.

[0167] Table 6 Results of indoor biological activity test on isoflualanam and chlorfenapyr against rice stem borer

[0168]

[0169]

[0170] Laboratory test results showed that combining isoflualanam and chlorfenapyr in an appropriate mass ratio exhibited a synergistic effect against the rice stem borer. When the mass ratio of isoflualanam to chlorfenapyr was 1:36-45:1, the co-toxicity coefficient against the rice stem borer was greater than 120, indicating a synergistic effect. When the mass ratio of isoflualanam to chlorfenapyr was 1:25-32:1, the co-toxicity coefficient against the rice stem borer was greater than 130, indicating a significant synergistic effect. When the mass ratio of isoflualanam to chlorfenapyr was 1:20-28:1, the co-toxicity coefficient against the rice stem borer was greater than 140, indicating a significant synergistic effect.

[0171] Example 3: Field efficacy test for controlling beet armyworm

[0172] Experimental location: The experiment was arranged in Sunjiazhai Village, Xiaodian District, Taiyuan City, Shanxi Province. The soil of the experimental site is sandy loam with medium fertility. The cultivation and management of the experimental site are in line with local scientific agricultural practices.

[0173] Test target: Beet armyworm.

[0174] Experimental crops: Cabbage.

[0175] Experimental design: The experiment set up 8 treatments, each treatment was repeated 4 times, and each plot was 15m 2 All experimental plots were arranged in random blocks.

[0176] Test method: The application time is the early stage of beet armyworm outbreak, the pesticide is diluted with water and sprayed on the leaves, the water consumption is 750kg / hm 2 The spray should be evenly and carefully applied on both sides of the cabbage leaves, so that the leaves are wet but not dripping. For the blank treatment, spray an equal amount of clean water.

[0177] Survey method: Conduct a fixed-point survey, sampling 5 points in each plot, surveying 10 plants at each point, for a total of 50 plants per plot. Investigate the base insect population before spraying, and the number of remaining insects 2, 5, and 10 days after spraying.

[0178] Calculation method of drug efficacy:

[0179]

[0180] Field efficacy test results:

[0181] Table 7 Results of field efficacy test on control of beet armyworm

[0182]

[0183]

[0184] It can be seen from the field efficacy tests in the above table that the combination of isoflualanam and diacylhydrazide compounds showed a good control effect on beet armyworm. Ten days after application, the control efficacy of the combination preparation on beet armyworm was 89.86% to 93.25%.

[0185] Example 4: Field efficacy test for controlling rice stem borer

[0186] The test site was a rice transplanting field in Liguan Village, Jiyang District, Jinan City, Shandong Province. The previous crop was wheat. The test site was flat and the soil fertility in each test plot was uniform.

[0187] Test target: Rice stem borer.

[0188] Experimental design: There are 8 treatments in the experiment, and each plot is 30m2. 2 All experimental plots were arranged in random blocks, and isolation zones were set between the plots.

[0189] Test method: When the rice stem borer reached the peak of egg hatching, the pesticide was applied using a 3WBJ-16DZ multifunctional backpack electric sprayer at a rate of 1000 spray points per 667m2. 2 Add 30L of water and spray evenly, the water consumption is 450L / hm2 2 There should be no rainfall on the day of application and within 3 days after application, and water should be retained for 1 week after application.

[0190] Efficacy investigation: 20 days after application, when the damage in the control area is obvious or the current damage is determined, parallel jumping sampling is carried out at 10 points, with 5 clumps at each point. A total of 50 clumps are investigated in each plot. The total number of plants and the number of dead hearts are recorded, and the dead heart rate and control effect are calculated.

[0191] Calculation method of drug efficacy:

[0192]

[0193] Field efficacy test results:

[0194] Table 8 Field efficacy test on control of rice stem borer

[0195]

[0196]

[0197] The results of field efficacy tests showed that the mixture of isoflualanam and dihydrazide compounds had a good control effect on the rice stem borer; 20 days after application, the comprehensive control effect of the compound preparation on the rice stem borer was above 88%, showing better persistent effect compared with the control single agent.

[0198] Although the present application describes specific embodiments in detail by way of example, the disclosure of the present application may adopt various modifications and alternative forms. However, it should be understood that the disclosure of the present application is not limited to the specific forms disclosed. On the contrary, the disclosure of the present application covers all modifications, equivalents and alternative forms within the scope of the disclosure of the present application, and the scope of the present application is limited by the appended claims and their legal equivalents.

Claims

1. An insecticidal composition containing a dihydrazide compound, characterized in that: The insecticide composition comprises active ingredient A and active ingredient B, wherein the active ingredient A is isoflualanam, and the active ingredient B is a dihydrazide compound, wherein the dihydrazide compound is either tebufenozide or chlorfenapyr. The mass ratio of isoflualanam to tebufenozide is 1:25-35:1; The mass ratio of isoflualanam to chlorfenapyr is 1:30 to 20:

1.

2. The insecticidal composition according to claim 1, characterized in that The mass ratio of isoflualanam to tebufenozide is 1:25 to 22:1; The mass ratio of isoflualanam to chlorfenapyr is 1:22-20:

1.

3. The insecticidal composition according to claim 2, characterized in that The mass ratio of isoflualanam to tebufenozide is 1:20 to 18:1; The mass ratio of isoflualanam to chlorfenapyr is 1:14-15:

1.

4. The insecticidal composition according to claim 1, characterized in that The total weight of the insecticide composition is calculated as 100 wt %, and the total weight of the active ingredient A and the active ingredient B accounts for 0.5% to 80% of the total weight of the insecticide composition.

5. The insecticidal composition according to claim 1, characterized in that In addition to the active ingredients, the insecticide composition also contains auxiliary ingredients allowed in pesticides, and the auxiliary ingredients are selected from one or more of wetting agents, dispersants, emulsifiers, thickeners, disintegrants, antifreeze agents, defoaming agents, solvents, preservatives, stabilizers, synergists or carriers.

6. The insecticidal composition according to claim 1, characterized in that The insecticide composition is prepared into a formulation form permitted by pesticides, and the formulation form is a solid preparation or a liquid preparation.

7. The insecticidal composition according to claim 6, characterized in that The solid preparation is a water-dispersible granule or a wettable powder, and the liquid preparation is a suspension, emulsifiable concentrate, aqueous emulsion, microemulsion or dispersible oil suspension.

8. Use of the insecticidal composition and the preparation thereof according to any one of claims 1 to 7 for controlling agricultural pests.

9. The use according to claim 8, characterized in that The pests are lepidoptera pests.

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