Application of tilletia foetida suspension in inhibition of fusarium solani

Abstract

The invention belongs to the technical field of biological prevention and control, and particularly relates to application of a Tilletia laevis Kuhn bacterial suspension in inhibition of fusarium solani. The Tilletia foetida suspension can be used for directly or indirectly inhibiting the growth of Fusarium solani (Fusarium solani) and preventing and treating root rot. Results of the embodiment show that under the condition of the same medicament concentration, compared with carbendazim, metalaxyl-M mancozeb and tilletia foetida suspension, growth of fusarium solani can be remarkably inhibited, and root rot can be prevented and treated.

Description

Technical Field

[0001] The invention belongs to the technical field of biological control, and particularly relates to application of a suspension of wheat smut fungus in preventing and controlling wheat root rot. Background Art

[0002] Fusarium solani, also known as Fusarium solani, is a common saprophyte in the soil and a conditionally pathogenic fungus. The disease it causes is called root rot. It is also the most common Fusarium that causes human infection. It is a transparent sporothrix widely found in soil, plants, and air. Fusarium has strong ecological adaptability and can even survive in deserts and Arctic regions.

[0003] Root rot is a common and destructive soil-borne disease in the process of plant growth. It mainly infects the roots of plants, causing water-soaked rot in the roots, loss of the ability to absorb water and nutrients, and ultimately leading to plant death. It is known as "plant cancer". Current technology mostly uses chemical pesticides to prevent and control root rot, but chemical pesticides are not only expensive, but long-term use will lead to increased resistance of pathogens, plant pesticide residues, and highly toxic residues that seriously affect human health and the natural environment. In addition, the vicious cycle of increasing disease and pesticide use seriously damages the ecological balance and pollutes the ecological environment. Therefore, there is an urgent need for a method for preventing and controlling root rot to replace chemical pesticides, inhibit Fusarium solani, and prevent and control root rot. Summary of the invention

[0004] The invention aims to avoid the use of chemical pesticides, provide an application of a wheat tartaria suspension in preventing and controlling root rot, inhibit the growth of Fusarium solani, and prevent and control the root rot.

[0005] The invention provides application of a wheat light tilletia (Tilletia laevis Kühn) bacterial suspension in inhibiting Fusarium solani (Fusarium solani).

[0006] The invention also provides application of wheat smut fungus suspension in preventing and treating root rot.

[0007] Preferably, the fungal suspension of T. tritici is one or more of a fungal suspension of T. tritici in the early mycelial development period, a fungal suspension of the primary basidiospore development period, a fungal suspension of the H body development period, and a fungal suspension of the secondary basidiospore development period.

[0008] Preferably, the method for preparing the bacterial suspension of wheat tart smut fungus in the early mycelial development stage comprises: mixing the winter spores of wheat tart smut fungus and soil extract to obtain a fungal culture solution, adding penicillin-streptomycin double antibiotics and culturing for 5 days, collecting the supernatant, and obtaining the bacterial suspension of wheat tart smut fungus in the early mycelial development stage.

[0009] Preferably, the method for preparing the bacterial suspension of wheat smut at the primary basidiospore development stage comprises: mixing the winter spores of wheat smut and soil extract to obtain a fungal culture solution, adding penicillin-streptomycin double antibiotics and culturing for 6 days, collecting the supernatant, and obtaining the bacterial suspension of wheat smut at the primary basidiospore development stage.

[0010] Preferably, the method for preparing the bacterial suspension of wheat tartaria in the H body development period comprises: mixing winter spores of wheat tartaria and soil extract to obtain fungal culture solution, adding penicillin-streptomycin double antibiotics and culturing for 7 days, collecting the supernatant, and obtaining the bacterial suspension of wheat tartaria in the H body development period.

[0011] Preferably, the method for preparing the bacterial suspension of wheat smut secondary basidiospore development period comprises: mixing the winter spores of wheat smut and soil extract to obtain fungal culture solution, adding penicillin-streptomycin double antibiotics and culturing for 9 days, collecting the supernatant, and obtaining the bacterial suspension of wheat smut secondary basidiospore development period.

[0012] Preferably, the preparation method of each 1000 mL of the soil leachate comprises: filtering 75 g of nutrient soil using 900 mL of distilled water at a temperature of 100° C., and diluting the obtained filtrate to 1000 mL using distilled water;

[0013] The teliospore concentration in the fungal culture solution is 3.5×10 6 ~5.5×10 6 Pieces / L.

[0014] Preferably, the culture temperature is 16°C, the pH value is 7.0, and the rotation speed is 150 rpm;

[0015] The volume ratio of the penicillin-streptomycin dual antibiotic to the fungal culture solution is 5:100;

[0016] The concentration of penicillin in the penicillin-streptomycin dual antibiotic is 100 IU / mL, and the concentration of streptomycin is 100 μg / mL.

[0017] The present invention also provides a bacterial agent for inhibiting Fusarium solani, which comprises a suspension of Tilletia tritici.

[0018] Beneficial effects:

[0019] The wheat smut fungus suspension can directly or indirectly inhibit the growth of Fusarium solani and prevent and treat root rot. The results of the embodiment show that under the same agent concentration conditions, compared with carbendazim and carbendazim·mancozeb, the wheat smut fungus suspension can significantly inhibit the growth of Fusarium solani and prevent and treat root rot. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required to be used in the embodiments are briefly introduced below.

[0021] Figure 1 It is Fusarium solani in culture;

[0022] Figure 2 It is the activated Fusarium solani;

[0023] Figure 3 The growth of Fusarium solani on the drug-containing plates; among them, the drug of A is the suspension of the winter spores of wheat light stinking powder fungus in the activation stage; the drug of B is the suspension of the first hyphae stage of wheat light stinking powder fungus; the drug of C is the suspension of the primary basidiospore stage of wheat light stinking powder fungus; the drug of D is the suspension of the H body stage of wheat light stinking powder fungus; the drug of E is the suspension of the secondary basidiospore stage of wheat light stinking powder fungus; the drug of F is carbendazim; the drug of G is fine armor frost·manganese zinc; the drug of H is water; the drug concentrations corresponding to the 1st, 2nd and 3rd row plates are low, medium and high concentrations of drugs respectively;

[0024] Figure 4 To compare the diameter of Fusarium solani after applying different concentrations of carbendazim and carbendazim·mancozeb;

[0025] Figure 5 To compare the diameter of Fusarium solani in different periods of time after applying different concentrations of wheat smut metabolites culture medium;

[0026] Figure 6 The results of comparison of inhibition percentage of Fusarium solani by applying different concentrations of carbendazim and carbendazim·mancozeb are shown in Table 1.

[0027] Figure 7 To compare the inhibition percentage of Fusarium solani by applying different concentrations of culture medium of metabolites of Tilletia oleracea at different stages;

[0028] Figure 8 The results of the toxicity test of carbendazim against Fusarium solani;

[0029] Fig. 9 The results of the toxicity test of the Jinjiashuang·Mancozeb to Fusarium solani;

[0030] Fig.10 This is the regression equation curve of the action of the suspension of wheat spores activated at the teliospore stage on Fusarium solani.

[0031] Fig.11 This is the regression equation curve of the effect of the suspension of early mycelial stage of Tilletia serrata on Fusarium solani.

[0032] Fig.12 This is the regression equation curve of the effect of the suspension of primary basidiospore stage of Tilletia oleracea on Fusarium solani.

[0033] Fig.13 This is the regression equation curve of the effect of the suspension of H-stage fungus of Tilletia oleracea on Fusarium solani.

[0034] Fig.14 This is the regression equation curve of the action of the fungal suspension of Tilletia tritici during the secondary basidiospore development period on Fusarium solani. DETAILED DESCRIPTION

[0035] The invention provides application of a wheat tinderia smut fungus suspension in inhibiting fusarium solani.

[0036] In the present invention, the wheat light tart smut fungus suspension is one or more of the wheat light tart smut fungus first hyphae development period fungus suspension, primary basidiospore development period fungus suspension, H body development period fungus suspension and secondary basidiospore development period fungus suspension, further preferably one or more of the first hyphae development period fungus suspension, primary basidiospore development period fungus suspension and secondary basidiospore development period fungus suspension, more preferably the secondary basidiospore development period fungus suspension. The present invention has no strict requirements on the source of the wheat light tart smut fungus, and a conventional selection can be used.

[0037] In the present invention, the preparation method of the bacterial suspension of wheat smut fungus in the early mycelium development period preferably includes: mixing the winter spores of wheat smut fungus and soil extract to obtain a fungal culture solution, adding penicillin-streptomycin double antibiotics and culturing for 5 days, collecting the supernatant, and obtaining the bacterial suspension of wheat smut fungus in the early mycelium development period.

[0038] In the present invention, the method for preparing each 1000 mL of the soil extract preferably comprises: filtering 75 g of nutrient soil using 900 mL of distilled water at a temperature of 100° C., and the obtained filtrate is fixed to 1000 mL using distilled water. The filtration of the present invention preferably uses 8 layers of gauze. The winter spore concentration in the fungal culture solution of the present invention is preferably 3.5×10 6 ~5.5×10 6 / L, more preferably 4×10 6 ~5×10 6 / L. The culture conditions of the present invention are preferably a temperature of 16°C, a pH of 7.0, and a rotation speed of 150rpm. The volume ratio of the penicillin-streptomycin dual antibiotic and the fungal culture solution of the present invention is preferably 5:100; the concentration of penicillin in the penicillin-streptomycin dual antibiotic is preferably 100IU / mL; the concentration of streptomycin in the penicillin-streptomycin dual antibiotic is preferably 100μg / mL.

[0039] The present invention collects a fungal suspension of wheat tart smut in the early mycelium development stage, which can inhibit the growth of Fusarium solani. The results of the embodiment show that when the fungal suspension of wheat tart smut in the early mycelium development stage is diluted to 20g / L-100g / L with distilled water, it can significantly inhibit the growth of Fusarium solani, and the inhibitory effect increases with the increase of concentration.

[0040] In the present invention, the preparation method of the bacterial suspension of the primary basidiospore development period of wheat stinking smut preferably comprises: mixing the winter spores of wheat stinking smut and soil extract to obtain a fungal culture solution, adding penicillin-streptomycin double antibiotics and culturing for 6 days, collecting the supernatant, and obtaining the bacterial suspension of the primary basidiospore development period of wheat stinking smut.

[0041] The preparation method of the soil extract of the present invention, the winter spore concentration in the fungal culture solution, the penicillin-streptomycin dual antibiotic and the volume and culture conditions of the fungal culture solution have been described above and will not be repeated here. The present invention collects the culture solution of the primary basidiospore suspension of wheat smut fungus, which can inhibit the growth of solani. The results of the embodiment show that when the primary basidiospore suspension of wheat smut fungus is diluted to 20g / L-100g / L with distilled water, it can significantly inhibit the growth of solani, and the inhibitory effect increases with the increase of concentration.

[0042] In the present invention, the preparation method of the bacterial suspension of wheat tartaria in the H body development period preferably includes: mixing the winter spores of wheat tartaria and soil extract to obtain a fungal culture solution, adding penicillin-streptomycin double antibiotics and culturing for 7 days, collecting the supernatant, and obtaining the bacterial suspension of wheat tartaria in the H body development period.

[0043] The preparation method of the soil extract of the present invention, the winter spore concentration in the fungal culture solution, the penicillin-streptomycin dual antibiotic and the volume and culture conditions of the fungal culture solution have been described above and will not be repeated here. The present invention collects the culture solution of the bacterial suspension of wheat light tinkling powder fungus in the H body stage, which can inhibit the growth of solani. The results of the embodiment show that when the culture solution of the bacterial suspension of wheat light tinkling powder fungus in the H body stage is diluted to 20g / L~100g / L with distilled water, it can significantly inhibit the growth of solani, and the inhibitory effect increases with the increase of concentration.

[0044] In the present invention, the preparation method of the bacterial suspension of wheat smut fungus in the secondary basidiospore development period preferably comprises: mixing the winter spores of wheat smut fungus and soil extract to obtain a fungal culture solution, adding penicillin-streptomycin double antibiotics and culturing for 9 days, collecting the supernatant, and obtaining the bacterial suspension of wheat smut fungus in the secondary basidiospore development period.

[0045] The preparation method of the soil extract of the present invention, the winter spore concentration in the fungal culture solution, the penicillin-streptomycin dual antibiotic and the volume and culture conditions of the fungal culture solution have been described above and will not be repeated here. The present invention collects the culture solution of the secondary basidiospore suspension of wheat smut fungus, which can inhibit the growth of solani. The results of the embodiment show that when the culture solution of the secondary basidiospore suspension of wheat smut fungus is diluted to 20g / L to 100g / L with distilled water, it can significantly inhibit the growth of solani, and the inhibitory effect increases with the increase of concentration.

[0046] In view of the effects of the wheat tinderia sutchuenensis suspension provided by the present invention, the use of the wheat tinderia sutchuenensis suspension in preventing and treating root rot also falls within the protection scope of the present invention.

[0047] In order to further illustrate the present invention, the application of the Tilletia oleracea suspension provided by the present invention in inhibiting Fusarium solani is described in detail below in conjunction with the accompanying drawings and examples, but they should not be construed as limiting the scope of protection of the present invention.

[0048] Example 1

[0049] 1. Materials and Methods

[0050] 1.1 Test materials

[0051] Ingredients: Fresh potatoes, wheat smut fungus;

[0052] Test pathogens: Fusarium solani;

[0053] 1.2 Instruments and reagents

[0054] 1.2.1 Instruments

[0055] High-pressure steam sterilizer, sterile operating table, constant temperature incubator, microwave oven, electronic balance, puncher, inoculation loop, alcohol lamp, 90mm sterile culture dish, conical flask, beaker, glass rod, blood cell counting plate, sterile centrifuge tube, cryopreservation tube, rubber-tipped dropper, sealing film, marker pen, label paper.

[0056] 1.2.2 Reagents

[0057] Glucose, agar, distilled water, 50% carbendazim wettable powder (Sichuan Guoguang Agrochemical Co., Ltd.), 68% carbendazim·manganese zinc water dispersible granules (Shandong United Pesticide Industry Co., Ltd.), and sterile water.

[0058] 2. Test methods

[0059] 2.1 Cultivation of Fusarium solani

[0060] 2.1.1 Preparation of PDA medium

[0061] After peeling the potatoes, weigh 200g and cut them into small pieces and put them into the pot. Add distilled water to 1L and heat to boil for 20-30min. After the potatoes can be poked with a glass rod, filter them with 4 layers of gauze on a measuring cup, retain the filtrate, and discard the residue. Add water to the filtrate to 1L. Put the filtrate back into the pot, heat it over low heat, add 20g glucose and 20g agar, and stir it continuously with a glass rod to prevent the agar from sticking to the bottom or overflowing. After the agar is completely dissolved, perform the fixed volume operation and add water to 1L. The fixed volume culture medium is divided into conical flasks, and the capacity of each conical flask does not exceed half of its volume. Sterilize it with high-pressure steam at 121℃ for 20 minutes, and then invert the PDA plate on the ultra-clean workbench that has been sterilized by ultraviolet. After the plate cools down, place it upside down to prevent water vapor from dripping into the culture medium and causing contamination.

[0062] 2.1.2 Activation and propagation of pathogens

[0063] The pathogen was taken out from the -20℃ refrigerator, placed at room temperature for 24h, and activated in PDA medium by plate streak method ( Figure 1 ), put it into a 28℃ mold incubator for culture and expansion ( Figure 2 ).

[0064] 2.2 Cultivation of winter spore germination of T. tritici and collection of metabolites at different stages

[0065] 2.2.1 Configuration of soil leachate

[0066] Weigh 75g of nutrient soil and place it on 8 layers of gauze. Pour boiled distilled water on the nutrient soil and filter it into a beaker. Finally, add distilled water to make up to 1000mL and divide it into 250mL conical flasks, 100mL per conical flask. Seal it with a breathable sealing film, sterilize it with high-pressure steam at 121℃ for 20min, and cool to room temperature for use.

[0067] 2.2.2 Cultivation of winter spore germination of T. tritici

[0068] The winter spores of wheat smut were added to the sterilized soil extract and mixed to obtain a winter spore concentration of 3.5×10 6 ~5.5×10 6 To each 100 mL of fungal culture solution, 0.5 mL of penicillin-streptomycin dual antibiotic (a mixture of penicillin (10000 IU) and streptomycin (10000 μg / mL) at 100 times the working concentration) was added for cultivation (temperature 16°C, pH 7.0, rotation speed 150 rpm).

[0069] 2.2.3 Collection of metabolites at different stages

[0070] The supernatants of soil extracts from the winter spore activation period (3 days of culture in fungal culture solution in step 2.2.2), the initial mycelial growth period (5 days of culture in fungal culture solution in step 2.2.2), the primary basidiospore period (6 days of culture in step 2.2.2), the H body period (7 days of culture in step 2.2.2) and the secondary basidiospore period (9 days of culture in step 2.2.2) of wheat light tartaria were extracted respectively, i.e., the bacterial suspensions at different stages of winter spore germination were stored in cryopreservation tubes, marked and placed in a 4°C refrigerator.

[0071] 2.3 Indoor toxicity test

[0072] Referring to the existing technology (Shen Qiong, Wu Junxin. Indoor screening of pesticides for the prevention and control of zucchini root rot [J]. Shanxi Agricultural Science, 2017, 45(03): 451-452+472.), each pesticide in the experimental group was prepared into three concentration gradients, and the original pesticide was prepared into a series of concentrations 10 times the required concentration with sterile water (carbendazim and methyl methacrylate·manganese zinc were set to 20, 50, and 100 mg / L respectively; the metabolite extracts of wheat smut at different stages were set to 20, 50, and 100 g / L); the control was sterile distilled water ( Figure 3 ); the experimental group agent and the control group sterile distilled water were mixed with sterile PDA culture medium at a temperature of about 60°C, and 4 parallel experiments were set up for the experimental group and the control group respectively;

[0073] Take the Fusarium solani cultured in a 28°C constant temperature incubator, make a 8 mm bacterial cake, inoculate it with the mycelium side facing down in the center of the culture medium plate, and culture it in a 28°C constant temperature incubator for 6 days.

[0074] (1) The diameter of the colonies grown in each culture dish was measured by the cross method, and the antibacterial rate was calculated according to the following formula. The results are shown in Table 1 and Figures 3 to 7 shown.

[0075] Inhibition rate = control colony diameter - experimental colony diameter / (control colony diameter - experimental bacterial cake diameter) × 100%

[0076] Table 1 Inhibitory effects of seven reagents on Fusarium solani

[0077]

[0078]

[0079] According to Table 1 and Figures 3 to 7It can be seen that among these 7 agents, for carbendazim, the antibacterial effect of the extract during the secondary basidiospore stage is relatively good regardless of whether it is at low or high concentrations. For the extract during the promycelium growth stage and the primary basidiospore stage, the antibacterial effect is good at high concentrations. For the two tested agents, carbendazim, metalaxyl-M·manganese zinc, and the suspensions of Tilletia foetida teliospores at five different growth stages, within a certain concentration range, they all have inhibitory effects on the pathogen Fusarium solani to varying degrees, and the inhibitory effect gradually strengthens with the increase in concentration. In the positive control group, carbendazim has the best inhibitory effect. When the concentration is 20 mg / L, the inhibition rate reaches 70.90%, while the inhibition rate of metalaxyl-M·manganese zinc is only 17.80%. When the concentration is 100 mg / L, the inhibition rate of carbendazim reaches 88.70%, and the inhibition rate of metalaxyl-M·manganese zinc also rises to 45.98%. Among the five stages of the extracellular metabolites of Tilletia foetida in the experimental group, when the concentration is 20 g / L, the inhibition rate gradually increases with the change of the stage. The activation stage of teliospores (22.91%) < promycelium growth stage (33.59%) < primary basidiospore stage (34.52%) < H body stage (39.94%) < secondary basidiospore stage (51.24%). When the concentration is 50 g / L, the activation stage of teliospores (27.40%) < primary basidiospore stage (38.70%) < H body stage (44.12%) < promycelium growth stage (48.45%) < secondary basidiospore stage (61.76%). When the concentration is 100 g / L, the activation stage of teliospores (32.97%) < H body stage (46.13%) < secondary basidiospore stage (69.35%) < promycelium growth stage (76.63%) < primary basidiospore stage (79.72%). When the concentration is low, the inhibition rate increases with the increase of the stage. When the concentration is high, the inhibition rates of the promycelium growth stage and the primary basidiospore stage are high, while the inhibition rate of the activation stage of teliospores is the lowest. With the gradual increase in concentration, the inhibition rates of the activation stage of teliospores and the H body stage change little, the inhibition rate of the secondary basidiospore stage rises steadily, and the inhibition rates of the promycelium growth stage and the primary basidiospore stage change greatly and have good inhibitory effects.

[0080] (2) Obtain the virulence regression equation based on the agent concentration and the mycelial growth inhibition rate. Respectively establish a virulence regression equation with the natural logarithm value of the agent concentration as the independent variable X and the probit value of the inhibition rate as the dependent variable Y. According to the least squares method, calculate the effective median concentration EC of the 7 agents by finding the probit value corresponding to an inhibition rate of 50%. 50 , and compare the virulence of the tested agents through the obtained EC 50 . The results are shown in Table 2 and Figures 8 to 14 .

[0081] Table 2 Toxicity test results of 7 reagents against Fusarium solani

[0082] Test drug Virulence regression equation Correlation coefficient <![CDATA[Effective medium concentration EC 50 > Carbendazim y=0.9052x+4.2877 0.7735 6.12 (mg / L) Nail Cream·Manganese Zinc y=1.1403x+2.5143 0.8622 151.28 (mg / L) Extract from winter spores during activation period (3 days) y=0.4283x+3.6919 0.9864 1132.66(g / L) Pre-hypha stage (5d) extract y=1.6086x+2.4072 0.9302 40.90(g / L) Extract from primary basidiospore stage (6d) y=1.6821x+2.2448 0.7520 43.44(g / L) Extract from H body stage (7 days) y=0.2283x+4.4520 0.9844 251.18(g / L) Extract from secondary basidiospore stage (9d) y=0.6823x+4.1419 1.0000 18.10(g / L)

[0083] According to Table 2 and Figures 8 to 14 it can be seen that in the positive control group, the lowest EC 50 value is that of carbendazim, which is 6.12 mg / L, and the EC 50 value of metalaxyl-M + mancozeb is 151.28 mg / L. In the experimental group, the lowest EC 50 value is that of the extract during the secondary basidiospore stage, which is 18.10 g / L, followed by the extract during the promycelium growth stage and the primary basidiospore stage, and the EC 50 values are 40.90 g / L and 43.44 g / L respectively. Under the condition of the same drug concentration, the inhibitory effect of the extract during the secondary basidiospore stage is the best, followed by the extract during the promycelium growth stage and the primary basidiospore stage. Generally speaking, for the effective median concentration EC 50 value, carbendazim < metalaxyl-M + mancozeb < extract during the secondary basidiospore stage < extract during the promycelium growth stage < extract during the primary basidiospore stage < extract during the H body stage < extract during the teliospore activation stage.

[0084] It can be seen from the above that the suspension of Tilletia foetida can inhibit the growth of Fusarium solani and prevent root rot.

[0085] Although the above embodiments have described the present invention in detail, they are only a part of the embodiments of the present invention, rather than all embodiments. People can also obtain other embodiments based on these embodiments without creative efforts, and these embodiments all fall within the protection scope of the present invention.

Claims

1. Application of Tilletia laevis Kühn suspension in inhibiting Fusarium solani.

2. Application of wheat smut fungus suspension in preventing and controlling root rot.

3. The use according to claim 1 or 2, characterized in that: The wheat tart smut fungus suspension is one or more of a wheat tart smut fungus suspension in the early mycelium development period, a primary basidiospore development period, a H body development period, and a secondary basidiospore development period.

4. The use according to claim 3, characterized in that: The preparation method of the fungal suspension of wheat tart smut fungus in the early mycelial development stage comprises: mixing winter spores of wheat tart smut fungus and soil extract to obtain fungal culture solution, adding penicillin-streptomycin double antibiotics and culturing for 5 days, collecting the supernatant, and obtaining the fungal suspension of wheat tart smut fungus in the early mycelial development stage.

5. The use according to claim 3, characterized in that: The preparation method of the bacterial suspension of wheat smut at the primary basidiospore development stage comprises: mixing winter spores of wheat smut and soil extract to obtain fungal culture solution, adding penicillin-streptomycin double antibiotics and culturing for 6 days, collecting the supernatant, and obtaining the bacterial suspension of wheat smut at the primary basidiospore development stage.

6. The use according to claim 3, characterized in that: The preparation method of the bacterial suspension of wheat tart smut fungus in the H body development period comprises: mixing winter spores of wheat tart smut fungus and soil extract to obtain fungal culture solution, adding penicillin-streptomycin double antibiotics and culturing for 7 days, collecting the supernatant, and obtaining the bacterial suspension of wheat tart smut fungus in the H body development period.

7. The use according to claim 3, characterized in that: The method for preparing the bacterial suspension of wheat smut secondary basidiospore development period comprises: mixing winter spores of wheat smut and soil extract to obtain fungal culture solution, adding penicillin-streptomycin double antibiotics and culturing for 9 days, collecting the supernatant, and obtaining the bacterial suspension of wheat smut secondary basidiospore development period.

8. The use according to any one of claims 4 to 7, characterized in that: The preparation method of each 1000 mL of the soil leachate comprises: filtering 75 g of nutrient soil using 900 mL of distilled water at a temperature of 100° C., and diluting the obtained filtrate to 1000 mL using distilled water; The concentration of teliospores in the fungal culture solution is 3.5×10 6 ~5.5×10 6 Pieces / L.

9. The use according to any one of claims 4 to 7, characterized in that: The culture temperature is 16°C, the pH value is 7.0, and the rotation speed is 150 rpm; The volume ratio of the penicillin-streptomycin dual antibiotic to the fungal culture solution is 5:100; The concentration of penicillin in the penicillin-streptomycin dual antibiotic is 100 IU / mL, and the concentration of streptomycin is 100 μg / mL.

10. A bacterial agent for inhibiting Fusarium solani, characterized in that: The bacterial agent includes a suspension of wheat smut fungus.

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