Anti-fatigue beverage containing taurine and preparation method thereof

Abstract

The preparation method comprises the following steps: respectively pulping and gelatinizing rice and glutinous rice to obtain rice gelatinized liquid and glutinous rice gelatinized liquid, performing enzymolysis on the rice gelatinized liquid through amylase and glucoamylase, performing partial fermentation through lactic acid bacteria, mixing the rice gelatinized liquid with the glutinous rice gelatinized liquid, and performing freeze-drying to obtain the taurine-containing anti-fatigue beverage. The taurine, the inositol, the vitamin B6 and the erythritol are added for blending, and filtering, filling and sterilizing are performed to obtain the product. The prepared anti-fatigue beverage can effectively resist fatigue, has a good taste, does not contain irritant components such as caffeine, and can reduce health risks possibly caused by long-term drinking of functional beverages.

Description

Technical Field

[0001] The invention belongs to the technical field of food and beverage processing, and particularly relates to an anti-fatigue beverage containing taurine and a preparation method thereof. Background Art

[0002] Functional beverages currently on the market generally add vitamins, glucose, minerals, electrolytes, lysine and other ingredients to water to achieve an anti-fatigue effect. However, in order to achieve quick results, caffeine and other stimulating ingredients are usually added to these anti-fatigue beverages. Long-term drinking of these ingredients may cause side effects such as palpitations and insomnia, which have adverse effects on human health. In addition, the taste of these beverages is often too stimulating and not suitable for all people. Therefore, there is a need to develop a functional beverage that is healthy and has a good taste.

[0003] Taurine is a special amino acid and an essential nutrient for the human body. At the same time, taurine plays a significant role in enhancing immunity and resisting fatigue. It can also promote intelligence and brain development, protect the heart and blood vessels, enhance immunity, promote carbohydrate and lipid metabolism, protect eyesight and promote visual development, maintain normal reproductive function, and have anti-oxidation and anti-aging effects, which helps maintain human health. Summary of the invention

[0004] The object of the present invention is to provide an anti-fatigue beverage containing taurine and a preparation method thereof, which can effectively resist fatigue and have a good taste, and can also reduce the health risks that may be caused by long-term drinking of functional beverages.

[0005] To achieve the above object, the present invention adopts the following technical solution: The invention discloses an anti-fatigue beverage containing taurine. The beverage is prepared by firstly beating and gelatinizing rice and glutinous rice to obtain rice gelatinized liquid and glutinous rice gelatinized liquid respectively, then hydrolyzing the rice gelatinized liquid with amylase and saccharifying enzyme, partially fermenting the rice gelatinized liquid with lactic acid bacteria, mixing the rice gelatinized liquid with glutinous rice gelatinized liquid, adding taurine, inositol, vitamin B6 and erythritol for blending, filtering, filling and sterilizing the beverage.

[0006] The preparation of the anti-fatigue beverage containing taurine specifically comprises the following steps: (1) adding water to rice and glutinous rice to fully soak them, beating them at high speed, and heating the obtained slurries to gelatinize them to obtain rice gelatinized liquid and glutinous rice gelatinized liquid; (2) adding amylase and saccharifying enzyme to the rice gelatinized liquid obtained in step (1) for enzymatic hydrolysis, then inactivating the enzymes and filtering to obtain a rice enzymatic hydrolyzate; (3) adding lactic acid bacteria to the rice hydrolyzate obtained in step (2) to carry out fermentation, and stopping the fermentation when the lactic acid content reaches 15-18 g / L to obtain rice fermentation liquid; (4) mixing the rice fermentation liquid obtained in step (3) with the glutinous rice gelatinized liquid obtained in step (1), and adding taurine, inositol, vitamin B6, and erythritol to prepare a prepared liquid; (5) The prepared liquid in step (4) is filtered, filled, and sterilized to obtain the anti-fatigue beverage containing taurine.

[0007] Furthermore, the weight ratio of rice to glutinous rice used in step (1) is (4-6):1.

[0008] Furthermore, the weight of water used in step (1) is 2-5 times the weight of rice or glutinous rice.

[0009] Furthermore, the heating gelatinization temperature in step (1) is 90-100°C and the time is 10-50 minutes.

[0010] Furthermore, the weight of the amylase used in step (2) is 0.3-0.5% of the weight of the rice gelatinization liquid.

[0011] Furthermore, the weight of the saccharifying enzyme used in step (2) is 0.1-0.3% of the weight of the rice gelatinization liquid. Furthermore, the temperature of the enzymatic hydrolysis in step (2) is 60-80°C and the time is 30-90 minutes.

[0012] Furthermore, the lactic acid bacteria in step (3) are one or more of Lactobacillus bulgaricus and Streptococcus thermophilus.

[0013] Furthermore, the weight of the lactic acid bacteria used in step (3) is 0.1-0.2% of the weight of the rice hydrolysate.

[0014] Furthermore, the fermentation temperature in step (3) is 25-40°C.

[0015] Furthermore, each 100 mL of the prepared solution obtained in step (4) contains 50-80 mg of taurine, 10-20 mg of inositol, 0.2-0.4 mg of vitamin B6, and 0-0.2 g of erythritol.

[0016] Furthermore, protein powders such as phycocyanin or oyster peptides may be added in step (4) to achieve a rapid anti-fatigue effect.

[0017] Lactic acid bacteria is a probiotic bacteria, which has the effects of promoting digestion, regulating intestinal flora, enhancing immunity, etc. The present invention adds lactic acid bacteria to the rice hydrolysate for fermentation, which not only makes the obtained beverage product have better health care effects due to the lactic acid bacteria, but also controls the fermentation degree for partial fermentation, so that the obtained beverage product has a better sweet and sour taste without adding sweeteners.

[0018] The present invention adds glutinous rice gelatinized liquid into rice fermented liquid for preparation, so that the prepared beverage product has a certain long-term anti-fatigue effect.

[0019] As a natural sweetener, erythritol has the characteristics of low calorie, high stability, harmonious sweetness, non-hygroscopicity, non-cariogenicity, and no gastrointestinal discomfort. The present invention uses erythritol in the beverage formula to adjust the taste, which can meet the different sweetness needs of consumers while avoiding the high energy and health problems caused by the use of traditional sweeteners such as white sugar.

[0020] The significant advantages of the present invention are: The anti-fatigue beverage containing taurine prepared by the present invention not only has an anti-fatigue effect, but also has a good taste, and does not contain irritating ingredients such as caffeine, and long-term drinking will not have adverse effects on health, and can meet consumers' dual needs for taste and health, and is suitable for people who need to maintain mental concentration and physical vitality for a long time, such as students, office workers, athletes, etc. DETAILED DESCRIPTION

[0021] An anti-fatigue beverage containing taurine, the preparation steps of which are as follows: (1) Weighing rice and glutinous rice in a weight ratio of (4-6):1, adding 2-5 times the weight of water to the rice and glutinous rice, respectively, soaking them fully and beating them at high speed, and then heating the obtained slurry at 90-100° C. for gelatinization for 10-50 min to obtain rice gelatinized liquid and glutinous rice gelatinized liquid; (2) adding 0.3-0.5% by weight of amylase and 0.1-0.3% by weight of saccharifying enzyme to the rice gelatinized liquid obtained in step (1), performing enzymatic hydrolysis at 60-80° C. for 30-90 min, then performing enzyme inactivation treatment at 100° C. for 5-8 min, and filtering to obtain a rice enzymatic hydrolyzate; (3) adding 0.1-0.2% by weight of lactic acid bacteria to the rice hydrolyzate obtained in step (2), and fermenting at 25-40° C. until the lactic acid content in the feed liquid reaches 15-18 g / L, thereby obtaining a rice fermentation liquid; (4) mixing the rice fermentation liquid obtained in step (3) with the glutinous rice gelatinized liquid obtained in step (1), and adding taurine, inositol, vitamin B6, and erythritol to prepare a prepared liquid; (5) The prepared liquid in step (4) is filtered, filled, and sterilized to obtain an anti-fatigue beverage containing taurine.

[0022] Wherein, the lactic acid bacteria used in step (3) are one or more of Lactobacillus bulgaricus and Streptococcus thermophilus.

[0023] Each 100 mL of the prepared solution obtained in step (4) contains 50-80 mg of taurine, 10-20 mg of inositol, 0.2-0.4 mg of vitamin B6, and 0-0.2 g of erythritol.

[0024] In step (4), protein powders such as phycocyanin or oyster peptide may be further added, and the amount added is calculated based on 10-100 mg per 100 mL of the prepared solution.

[0025] In order to make the contents of the present invention easier to understand, the technical solution of the present invention is further described below in conjunction with specific implementation methods, but the present invention is not limited thereto.

[0026] The lactic acid bacteria used are prepared by mixing Lactobacillus bulgaricus and Streptococcus thermophilus in a weight ratio of 1:1.

[0027] Example 1 (1) Weighing rice and glutinous rice at a weight ratio of 4:1, adding water twice the weight of the rice and glutinous rice respectively, soaking them fully and beating them at high speed, and then heating the obtained slurry at 90-100° C. for gelatinization for 20-50 min to obtain rice gelatinized liquid and glutinous rice gelatinized liquid; (2) adding 0.4% by weight of amylase and 0.1% by weight of saccharifying enzyme to the rice gelatinized liquid obtained in step (1), performing enzymatic hydrolysis at 60-80° C. for 60 min, then performing enzyme inactivation treatment at 100° C. for 8 min, and filtering to obtain a rice enzymatic hydrolyzate; (3) adding 0.1% by weight of lactic acid bacteria to the rice hydrolyzate obtained in step (2), and fermenting at 25-40° C. until the lactic acid content in the feed liquid reaches 15-18 g / L, thereby obtaining a rice fermentation liquid; (4) mixing the rice fermentation liquid obtained in step (3) with the glutinous rice gelatinized liquid obtained in step (1), and adding taurine, inositol, vitamin B6, and erythritol to prepare a prepared liquid; each 100 mL of the prepared liquid contains 80 mg of taurine, 20 mg of inositol, and 0.2 mg of vitamin B6; (5) The prepared liquid in step (4) is filtered, filled, and sterilized to obtain an anti-fatigue beverage.

[0028] Take 50 mL of the obtained anti-fatigue beverage and place it in a colorless and transparent container, and observe it in a bright place. It can be seen that the product is milky white, with a uniform and delicate texture and no stratification. It has a natural smell and no peculiar smell at room temperature, and tastes sweet and sour.

[0029] Example 2 (1) Weighing rice and glutinous rice at a weight ratio of 4:1, adding water twice the weight of the rice and glutinous rice respectively, soaking them fully and beating them at high speed, and then heating the obtained slurry at 90-100° C. for gelatinization for 20-50 min to obtain rice gelatinized liquid and glutinous rice gelatinized liquid; (2) adding 0.4% by weight of amylase and 0.1% by weight of saccharifying enzyme to the rice gelatinized liquid obtained in step (1), performing enzymatic hydrolysis at 60-80° C. for 60 min, then performing enzyme inactivation treatment at 100° C. for 8 min, and filtering to obtain a rice enzymatic hydrolyzate; (3) adding 0.1% by weight of lactic acid bacteria to the rice hydrolyzate obtained in step (2), and fermenting at 25-40° C. until the lactic acid content in the feed liquid reaches 15-18 g / L, thereby obtaining a rice fermentation liquid; (4) mixing the rice fermentation liquid obtained in step (3) with the glutinous rice gelatinized liquid obtained in step (1), and adding taurine, phycocyanin, inositol, vitamin B6, and erythritol to prepare a prepared liquid; each 100 mL of the prepared liquid contains 60 mg of taurine, 20 mg of phycocyanin, 20 mg of inositol, and 0.2 mg of vitamin B6; (5) The prepared liquid in step (4) is filtered, filled, and sterilized to obtain an anti-fatigue beverage.

[0030] Example 3 (1) Weighing rice and glutinous rice at a weight ratio of 4:1, adding water twice the weight of the rice and glutinous rice respectively, soaking them fully and beating them at high speed, and then heating the obtained slurry at 90-100° C. for gelatinization for 20-50 min to obtain rice gelatinized liquid and glutinous rice gelatinized liquid; (2) adding 0.4% by weight of amylase and 0.1% by weight of saccharifying enzyme to the rice gelatinized liquid obtained in step (1), performing enzymatic hydrolysis at 60-80° C. for 60 min, then performing enzyme inactivation treatment at 100° C. for 8 min, and filtering to obtain a rice enzymatic hydrolyzate; (3) adding 0.1% by weight of lactic acid bacteria to the rice hydrolyzate obtained in step (2), and fermenting at 25-40° C. until the lactic acid content in the feed liquid reaches 15-18 g / L, thereby obtaining a rice fermentation liquid; (4) mixing the rice fermentation liquid obtained in step (3) with the glutinous rice gelatinized liquid obtained in step (1), and adding taurine, oyster peptide, inositol, vitamin B6, and erythritol to prepare a prepared liquid; each 100 mL of the prepared liquid contains 60 mg of taurine, 20 mg of oyster peptide, 20 mg of inositol, and 0.2 mg of vitamin B6; (5) The prepared liquid in step (4) is filtered, filled, and sterilized to obtain an anti-fatigue beverage.

[0031] Comparative Example (1) adding water twice its weight to the rice, fully soaking the rice and beating the rice at high speed, and then heating the resulting slurry at 90-100° C. for 20-50 min to obtain a rice gelatinized liquid; (2) adding 0.4% by weight of amylase and 0.1% by weight of saccharifying enzyme to the rice gelatinized liquid obtained in step (1), performing enzymatic hydrolysis at 60-80° C. for 60 min, then performing enzyme inactivation treatment at 100° C. for 8 min, and filtering to obtain a rice enzymatic hydrolyzate; (3) adding 0.1% by weight of lactic acid bacteria to the rice hydrolyzate obtained in step (2), and fermenting at 25-40° C. until the lactic acid content in the feed liquid reaches 15-18 g / L, thereby obtaining a rice fermentation liquid; (4) adding taurine, inositol, vitamin B6, and erythritol to the rice fermentation liquid obtained in step (3) to prepare a prepared liquid; each 100 mL of the prepared liquid contains 80 mg of taurine, 20 mg of inositol, and 0.2 mg of vitamin B6; (5) The prepared liquid in step (4) is filtered, filled, and sterilized to obtain an anti-fatigue beverage.

[0032] Fatigue test 50 adult healthy male mice, weighing 18-22g, were selected and randomly divided into CK group and experimental groups A, B, C, and D, 10 mice / group. The CK group was gavaged with distilled water, and the experimental groups AD were gavaged with the anti-fatigue beverages prepared in Examples 1-3 and the comparative examples, respectively, at a dose of 0.8mL / 20g, for 30 consecutive days. 30 minutes after the last gavage, 5% of the body weight of lead was loaded on the tail root of the mouse, and then the weighted mouse was placed in a swimming box for swimming (water depth not less than 30cm, water temperature 25±1℃), and the time from the start of swimming to death of the mouse was recorded as the swimming time of the mouse. The results are shown in Table 1.

[0033] Table 1 Comparison of swimming time of mice in different experimental groups

[0034] As can be seen from Table 1, compared with the CK group that was gavaged with distilled water, the experimental groups that were gavaged with anti-fatigue beverages could effectively prolong the weighted swimming time of mice, and the anti-fatigue beverage prepared with glutinous rice gelatin liquid had a longer-lasting anti-fatigue effect.

[0035] The above description is only a preferred embodiment of the present invention. All equivalent changes and modifications made according to the scope of the patent application of the present invention should fall within the scope of the present invention.

Claims

1. A method for preparing an anti-fatigue beverage containing taurine, characterized in that: Firstly, rice and glutinous rice are pulped and gelatinized to obtain rice gelatinized liquid and glutinous rice gelatinized liquid respectively; then, the rice gelatinized liquid is hydrolyzed by amylase and saccharifying enzyme and partially fermented by lactic acid bacteria, and then mixed with glutinous rice gelatinized liquid; taurine, inositol, vitamin B6 and erythritol are added for preparation; and after filtering, filling and sterilization, the anti-fatigue beverage containing taurine is obtained.

2. The method for preparing the anti-fatigue beverage containing taurine according to claim 1, characterized in that: It specifically includes the following steps: (1) adding water to rice and glutinous rice to fully soak them, beating them at high speed, and heating the obtained slurries to gelatinize them to obtain rice gelatinized liquid and glutinous rice gelatinized liquid; (2) adding amylase and saccharifying enzyme to the rice gelatinized liquid obtained in step (1) for enzymatic hydrolysis, then inactivating the enzymes and filtering to obtain a rice enzymatic hydrolyzate; (3) adding lactic acid bacteria to the rice hydrolyzate obtained in step (2) to carry out fermentation, and stopping the fermentation when the lactic acid content reaches 15-18 g / L to obtain rice fermentation liquid; (4) mixing the rice fermentation liquid obtained in step (3) with the glutinous rice gelatinized liquid obtained in step (1), and adding taurine, inositol, vitamin B6, and erythritol to prepare a prepared liquid; (5) The prepared liquid in step (4) is filtered, filled, and sterilized to obtain the anti-fatigue beverage containing taurine.

3. The method for preparing the anti-fatigue beverage containing taurine according to claim 2, characterized in that: The weight ratio of rice to glutinous rice used in step (1) is (4-6):1; the weight of water used is 2-5 times the weight of rice or glutinous rice.

4. The method for preparing the anti-fatigue beverage containing taurine according to claim 2, characterized in that: The heating gelatinization temperature in step (1) is 90-100°C and the time is 10-50 minutes.

5. The method for preparing the anti-fatigue beverage containing taurine according to claim 2, characterized in that: The weight of the amylase used in step (2) is 0.3-0.5% of the weight of the rice gelatinized liquid, and the weight of the saccharifying enzyme used is 0.1-0.3% of the weight of the rice gelatinized liquid; the temperature of the enzymolysis is 60-80° C., and the time is 30-90 min.

6. The method for preparing the anti-fatigue beverage containing taurine according to claim 2, characterized in that: The weight of the lactic acid bacteria used in step (3) is 0.1-0.2% of the weight of the rice hydrolysate; the lactic acid bacteria are one or more of Lactobacillus bulgaricus and Streptococcus thermophilus.

7. The method for preparing the anti-fatigue beverage containing taurine according to claim 2, characterized in that: The fermentation temperature in step (3) is 25-40°C.

8. The method for preparing the anti-fatigue beverage containing taurine according to claim 2, characterized in that: Each 100 mL of the prepared solution obtained in step (4) contains 50-80 mg of taurine, 10-20 mg of inositol, 0.2-0.4 mg of vitamin B6, and 0-0.2 g of erythritol.

9. An anti-fatigue beverage containing taurine prepared by the method as claimed in claim 1.

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