Composition and product with effects of maintaining beauty and keeping young as well as preparation method and application of composition

Abstract

The invention relates to the technical field of food, in particular to a composition and a product with effects of maintaining beauty and keeping young as well as a preparation method and application of the composition. The composition is prepared from the following components in parts by weight: 5 to 12 parts of fish collagen peptide, 0.1 to 0.5 part of III type collagen peptide, 0.1 to 0.5 part of ray collagen peptide, 1 to 3 parts of collagen tripeptide, 0.1 to 0.5 part of cubilose peptide, 0.1 to 0.3 part of casein peptide, 0.1 to 0.5 part of fish maw oligopeptide and 0.01 to 0.05 part of red currant and black currant mixed powder. The skin care product has the advantages of good skin moisture and elasticity improving and wrinkle removing effects and high safety.

Description

Technical Field

[0001] The present invention relates to the field of food technology, and in particular to a composition and product with beauty and skin care effects, a preparation method of the composition and application thereof. Background Art

[0002] Dermal fibroblasts synthesize and secrete collagen and elastin, generate collagen fibers and elastic fibers, and also synthesize and secrete matrix components such as glycosaminoglycans and glycoproteins. Collagen fibers are the main components of dermal tissue. They are tough and have strong tensile strength, which allows the skin to withstand large tensile forces, but lack elasticity. Elastic fibers make the skin elastic and easily return to its original state after being pulled. The matrix fills between fibers and cells. Collagen fibers and elastic fibers in dermal tissue are intertwined with each other and buried in the matrix, maintaining the firmness and elasticity of the skin. The fibrous tissue and matrix in the dermis are closely related to the density, fullness, relaxation, wrinkles and dryness of the skin.

[0003] Due to natural aging and photoaging, the number and activity of dermal fibroblasts decrease with age, the synthesis of collagen, elastin and hyaluronic acid decreases, the degradation of collagen and elastic fibers in the skin accelerates, and their structures become abnormal or degenerated, causing the skin to become dry, sagging, wrinkled, and other problems. In particular, photoaging causes a large amount of matrix metalloproteinases to specifically degrade various collagens and elastin, thereby aggravating problems such as dryness, sagging, and wrinkles.

[0004] Currently, there are relatively few beauty and health foods on the market. They have single nutrition, limited functions, unsatisfactory hydrating and beauty effects, and generally contain chemical agents that may harm the human body.

[0005] Chinese patent publication number CN106901364A discloses a beauty and skin care health food and its preparation method. The health food is mainly made of the following raw materials in percentage by mass: 45-50% soy protein oligopeptide, 2-5% angelica extract, 2-5% grape seed extract, and 2-5% oral hyaluronic acid. Soy protein oligopeptide combined with angelica extract and grape seed extract enhances the freckle and spot removal effects of health food; soy protein oligopeptide combined with grape seed extract and hyaluronic acid enhances the effect of health food in delaying aging, preventing and eliminating wrinkles; soy protein oligopeptide combined with hyaluronic acid enhances the effect of health food in improving skin dullness and restoring skin luster.

[0006] Chinese patent publication number CN104489700A discloses a composition with the functions of improving skin moisture, elasticity and wrinkle removal. The active ingredients are mainly collagen peptides and elastin peptides and Chinese herbal medicine extracts. The Chinese herbal medicine extracts are prepared from astragalus, polygonatum, rehmannia and rose as raw materials.

[0007] Although the above products have improved the skin's moisture, elasticity and wrinkle removal effects to a certain extent, the effects still need to be further improved. In addition, the above products use medicinal ingredients such as angelica, astragalus, and raw rehmannia, which may have certain side effects on the human body.

[0008] Therefore, it is very necessary to develop a composition and product with beauty and skin care effects that can solve the above technical problems, a preparation method of the composition and its application. Summary of the invention

[0009] The purpose of the present invention is to overcome the deficiencies of the prior art and provide a composition and product having good skin moisture, elasticity and wrinkle removal effects and high safety, as well as a preparation method and application of the composition.

[0010] The present invention is achieved through the following technical solutions: The first aspect of the present invention provides a composition with beauty and skin care effects, characterized in that it comprises the following components in parts by weight: 5-12 parts of fish collagen peptide, 0.1-0.5 parts of type III collagen peptide, 0.1-0.5 parts of skate collagen peptide, 1-3 parts of collagen tripeptide, 0.1-0.5 parts of bird's nest peptide, 0.1-0.3 parts of casein peptide, 0.1-0.5 parts of fish maw oligopeptide, and 0.01-0.05 parts of red and black currant mixed powder; The preparation method of the fish maw oligopeptide comprises the following steps: (1) crushing the fish maw, adding water, and homogenizing to obtain a homogenate; (2) adding enzyme to the homogenate to perform enzymolysis, and then inactivating the enzyme to obtain enzymolysis solution; (3) After centrifuging the enzymatic hydrolysate, the enzymatic hydrolysate is separated by a membrane separation method and dried; The fish bladders in step (1) are sourced from croaker, yellow croaker and silver carp, and the mass ratio of the fish bladders of the three fish is 2-4:1-3:1; In step (2), alkaline protease, papain and bromelain are added in sequence for enzymolysis.

[0011] In some embodiments of the present invention, the composition is an oral composition.

[0012] In some embodiments of the present invention, the mass ratio of the collagen tripeptide, the bird's nest peptide and the fish maw oligopeptide is 10:1-2:1-2.

[0013] In some embodiments of the present invention, the mass ratio of the fish maw to water in step (1) is 1:5-10.

[0014] In some embodiments of the present invention, the pH of the homogenate is adjusted to 6.0-8.0 before enzymatic hydrolysis in step (2).

[0015] In some embodiments of the present invention, the amount of the enzyme used in step (2) is 2-4% of the mass of the homogenate.

[0016] In some embodiments of the present invention, the enzymes in step (2) are alkaline protease, papain and bromelain, and the mass ratio of the three is 2-4:1-3:4-6.

[0017] In some embodiments of the present invention, the enzymatic hydrolysis temperature in step (2) is 45-55° C., and the enzymatic hydrolysis time is 1.5-3 h.

[0018] In some preferred embodiments of the present invention, the specific process of enzymatic hydrolysis is as follows: first add alkaline protease for enzymatic hydrolysis for 0.5-1h, then add papain for enzymatic hydrolysis for 0.5-1h, and finally add bromelain for enzymatic hydrolysis for 0.5-1h.

[0019] In some embodiments of the present invention, the specific method of inactivating the enzyme in step (2) is not limited, such as keeping the enzyme in a boiling water bath for 10-20 minutes after enzymatic hydrolysis.

[0020] In some embodiments of the present invention, in step (3), the precipitate is removed by centrifugation to obtain a supernatant for membrane separation.

[0021] In some embodiments of the present invention, the molecular weight cutoff of the membrane in step (3) is 3000-5000Da.

[0022] In some embodiments of the present invention, the drying method in step (3) is spray drying.

[0023] The second aspect of the present invention provides a method for preparing the above-mentioned composition, comprising the following steps: the components are mixed uniformly to obtain the composition.

[0024] The third aspect of the present invention provides a product, comprising the above-mentioned composition and auxiliary materials.

[0025] In some embodiments of the present invention, the auxiliary materials include but are not limited to at least one of fruit and vegetable juice, emblica powder, yeast extract, niacinamide, acidulants, thickeners, preservatives, food flavors and sweeteners.

[0026] In some embodiments of the present invention, the fruit and vegetable juice includes but is not limited to at least one of blood orange concentrated juice, concentrated apple juice, grapefruit juice, red grapefruit concentrated juice, and purple carrot juice.

[0027] In some embodiments of the present invention, the thickener includes but is not limited to xanthan gum.

[0028] In some embodiments of the present invention, the preservative includes but is not limited to potassium sorbate.

[0029] In some embodiments of the present invention, the sweetener includes but is not limited to at least one of erythritol and sucralose.

[0030] In some embodiments of the present invention, the acidulant includes but is not limited to at least one of DL-malic acid, citric acid and sodium citrate.

[0031] In some embodiments of the present invention, the product is a food or a health product.

[0032] In some embodiments of the present invention, the product comprises, by weight, 30-50 parts of the composition having beauty and skin care effects, 1-5 parts of blood orange concentrated juice, 5-10 parts of concentrated apple juice, 1-5 parts of grapefruit juice, 0.5-2 parts of red grapefruit concentrated juice, 0.5-2 parts of purple carrot juice, 0.1-1 parts of emblica powder, 0.1-1 parts of yeast extract, 0.01-0.015 parts of niacinamide, 0.5-2 parts of DL-malic acid, 0.5-2 parts of citric acid, 0.2-2 parts of sodium citrate, 0.2-1 parts of xanthan gum, 0.01-0.1 parts of potassium sorbate, 0.05-0.5 parts of food flavoring, 1-10 parts of erythritol and 0.05-0.2 parts of sucralose.

[0033] The preparation process of the product comprises the following steps: S1. Dissolve the sweetener, thickener and the composition in 4-6 times (based on the amount of the composition) of water at 80-90° C., and stir until completely dissolved to obtain a mixture A; S2: adding emblica powder, yeast extract and nicotinamide to mixture A, and slowly adding fruit and vegetable juice, preservatives and acidulant after complete dissolution, and mixing evenly to obtain mixture B; S3 is prepared by adding food flavoring into mixture B and sterilizing the mixture.

[0034] The fourth aspect of the present invention provides the use of the above composition in preparing a product with beauty and skin care effects.

[0035] In some embodiments of the present invention, the beauty and skin care includes but is not limited to at least one of improving skin moisture status and improving wrinkles.

[0036] In some embodiments of the present invention, the product is a food or a health product.

[0037] The beneficial effects of the present invention are: The invention adopts a specific fish maw type to prepare fish maw oligopeptide. The prepared fish maw oligopeptide is applied to beauty and skin care products, and has significant effects of improving skin moisture condition and wrinkles.

[0038] In addition, when fish maw oligopeptides are enzymatically hydrolyzed using specific enzymes in a specific order, their effects on improving skin moisture and wrinkles are further enhanced.

[0039] The present invention further optimizes the composition of the peptide-containing composition, especially the compound use of collagen tripeptide, bird's nest peptide and fish maw oligopeptide in a specific ratio, which significantly improves the effect of improving skin moisture condition and improving wrinkles. DETAILED DESCRIPTION

[0040] The present invention will be further described below in conjunction with specific embodiments, and the advantages and features of the present invention will become clearer as the description proceeds. However, these embodiments are exemplary only and do not constitute any limitation to the scope of the present invention. It should be understood by those skilled in the art that the details and forms of the technical solution of the present invention may be modified or replaced without departing from the spirit and scope of the present invention, but these modifications and replacements all fall within the scope of protection of the present invention.

[0041] The auxiliary materials used in the present invention are all conventional commercially available raw materials, and the following auxiliary material raw material sources are illustrative: Blood orange juice concentrate was purchased from Wuhu Lequan Biotechnology Co., Ltd., concentrated apple juice was purchased from Sanmenxia Yuanfenduo Juice Co., Ltd., grapefruit juice was purchased from Shanghai Lecheng Biotechnology Co., Ltd., red grapefruit juice concentrate was purchased from Tianjin Zhenruguo Food Industry Co., Ltd., purple carrot juice was purchased from Henan Zhongda Hengyuan Biotechnology Co., Ltd., emblica powder was purchased from Shanghai Nuoshen Food Trading Co., Ltd., yeast extract was purchased from Shanghai Guanshuo Biotechnology Co., Ltd., niacinamide was purchased from Shanghai Licheng Nutrition Technology Co., Ltd., xanthan gum was purchased from Hangzhou Weichun Food Accessories Co., Ltd., and food flavors were purchased from Hasegawa Flavors (Shanghai) Co., Ltd.

[0042] The sources of raw materials used in the embodiments and comparative examples of the present invention are shown in Table 1.

[0043] Table 1 Raw material source information

[0044] Example 1 A composition with beauty and skin care effects, comprising the following components by weight: 5 parts of fish collagen peptide, 0.1 parts of type III collagen peptide, 0.1 parts of skate collagen peptide, 1 part of collagen tripeptide, 0.1 parts of bird's nest peptide, 0.1 parts of casein peptide, 0.1 parts of fish maw oligopeptide, and 0.01 parts of red and black currant mixed powder. The raw material components are uniformly mixed to obtain the composition.

[0045] The preparation method of the fish maw oligopeptide comprises the following steps: (1) crushing the fish maw, adding 5 times the amount (m / m) of water, and homogenizing to obtain a homogenate; the fish maws are sourced from croaker, yellow croaker and silver carp, and the mass ratio of the maws of the three fish is 2:1:1; (2) The pH of the homogenate was adjusted to 6.0, and alkaline protease was first added for enzymatic hydrolysis for 0.5 h, papain was then added for enzymatic hydrolysis for 0.5 h, and bromelain was finally added for enzymatic hydrolysis for 0.5 h. The enzymatic hydrolysis temperature was 45 °C, and the mass ratio of alkaline protease, papain and bromelain was 2:1:4. The total amount of enzymes used was 2% of the mass of the homogenate, and the enzyme solution was obtained after the enzymes were inactivated. (3) The enzymatic hydrolysate is centrifuged to remove the precipitate, and the supernatant is separated by a membrane with a molecular weight cutoff of 3000 Da, and then spray-dried.

[0046] Example 2 A composition with beauty and skin care effects, the components are as follows by weight: 12 parts of fish collagen peptide, 0.5 parts of type III collagen peptide, 0.5 parts of skate collagen peptide, 3 parts of collagen tripeptide, 0.5 parts of bird's nest peptide, 0.3 parts of casein peptide, 0.5 parts of fish maw oligopeptide, 0.05 parts of red and black currant mixed powder. The raw material components are evenly mixed to obtain the composition.

[0047] The preparation method of the fish maw oligopeptide comprises the following steps: (1) crushing the fish maw, adding 10 times the amount (m / m) of water, and homogenizing to obtain a homogenate; the fish maws are sourced from croaker, yellow croaker and silver carp, and the mass ratio of the maws of the three fish is 4:3:1; (2) The pH of the homogenate was adjusted to 8.0, and alkaline protease was first added for enzymatic hydrolysis for 1 h, papain was then added for enzymatic hydrolysis for 1 h, and bromelain was finally added for enzymatic hydrolysis for 1 h. The enzymatic hydrolysis temperature was 55°C, and the mass ratio of alkaline protease, papain and bromelain was 4:3:6. The total amount of enzyme was 4% of the mass of the homogenate, and the enzyme was inactivated to obtain the enzymatic hydrolyzate. (3) The enzymatic hydrolysate is centrifuged to remove the precipitate, and the supernatant is separated by a membrane with a molecular weight cutoff of 5000 Da, and then spray-dried.

[0048] Example 3 A composition with beauty and skin care effects, the components are as follows, calculated by weight: 9 parts of fish collagen peptide, 0.3 parts of type III collagen peptide, 0.3 parts of skate collagen peptide, 2 parts of collagen tripeptide, 0.3 parts of bird's nest peptide, 0.2 parts of casein peptide, 0.3 parts of fish maw oligopeptide, 0.03 parts of red and black currant mixed powder. The raw material components are evenly mixed to obtain the composition.

[0049] The preparation method of the fish maw oligopeptide comprises the following steps: (1) crushing the fish maw and adding 8 times the volume (m / m) of water, and homogenizing to obtain a homogenate; the fish maws are sourced from croaker, yellow croaker and silver carp, and the mass ratio of the maws of the three fish is 3:2:1; (2) The pH of the homogenate was adjusted to 7.0, and alkaline protease was first added for enzymatic hydrolysis for 0.75 h, papain was then added for enzymatic hydrolysis for 0.75 h, and bromelain was finally added for enzymatic hydrolysis for 0.75 h. The enzymatic hydrolysis temperature was 50 °C, and the mass ratio of alkaline protease, papain and bromelain was 3:2:5. The total amount of enzyme was 3% of the mass of the homogenate, and the enzyme was inactivated to obtain the enzymatic hydrolyzate. (3) The enzymatic hydrolysate is centrifuged to remove the precipitate, and the supernatant is separated by a membrane with a molecular weight cutoff of 4000 Da, and then spray-dried.

[0050] Application Example 1 A beauty and skin care product, comprising 50 parts of the composition of Example 1, 5 parts of blood orange concentrated juice, 10 parts of concentrated apple juice, 5 parts of grapefruit juice, 2 parts of red grapefruit concentrated juice, 2 parts of purple carrot juice, 1 part of emblica powder, 1 part of yeast extract, 0.015 parts of niacinamide, 2 parts of DL-malic acid, 2 parts of citric acid, 2 parts of sodium citrate, 1 part of xanthan gum, 0.1 parts of potassium sorbate, 0.5 parts of food flavoring, 10 parts of erythritol and 0.2 parts of sucralose.

[0051] The preparation process of the product comprises the following steps: S1. Dissolve erythritol, sucralose, xanthan gum and the composition in 6 times the amount (based on the amount of the composition) of water at 80° C., and stir until completely dissolved to obtain a mixture A; S2: adding emblica powder, yeast extract and niacinamide to mixture A, and slowly adding blood orange juice concentrate, concentrated apple juice, grapefruit juice, red grapefruit juice concentrate, purple carrot juice, potassium sorbate, DL-malic acid, citric acid and sodium citrate after the mixture is completely dissolved, and mixing evenly to obtain mixture B; S3 is prepared by adding food flavoring into mixture B and sterilizing the mixture.

[0052] Application Example 2 A beauty and skin care product, comprising 30 parts of the composition of Example 2, 1 part of blood orange concentrated juice, 5 parts of concentrated apple juice, 1 part of grapefruit juice, 0.5 parts of red grapefruit concentrated juice, 0.5 parts of purple carrot juice, 0.1 parts of emblica powder, 0.1 parts of yeast extract, 0.01 parts of niacinamide, 0.5 parts of DL-malic acid, 0.5 parts of citric acid, 0.2 parts of sodium citrate, 0.2 parts of xanthan gum, 0.01 parts of potassium sorbate, 0.05 parts of food flavoring, 1 part of erythritol and 0.05 parts of sucralose.

[0053] The preparation process of the product comprises the following steps: S1. Dissolve erythritol, sucralose, xanthan gum and the composition in 4 times the amount (based on the amount of the composition) of water at 90° C., and stir until completely dissolved to obtain a mixture A; S2: adding emblica powder, yeast extract and niacinamide to mixture A, and slowly adding blood orange juice concentrate, concentrated apple juice, grapefruit juice, red grapefruit juice concentrate, purple carrot juice, potassium sorbate, DL-malic acid, citric acid and sodium citrate after the mixture is completely dissolved, and mixing evenly to obtain mixture B; S3 is prepared by adding food flavoring into mixture B and sterilizing the mixture.

[0054] Application Example 3 A beauty and skin care product, comprising 40 parts of the composition of Example 3, 3 parts of blood orange concentrated juice, 7 parts of concentrated apple juice, 3 parts of grapefruit juice, 1 part of red grapefruit concentrated juice, 1 part of purple carrot juice, 0.5 parts of emblica powder, 0.5 parts of yeast extract, 0.01 parts of niacinamide, 1 part of DL-malic acid, 1 part of citric acid, 1 part of sodium citrate, 0.5 parts of xanthan gum, 0.05 parts of potassium sorbate, 0.2 parts of food flavoring, 5 parts of erythritol and 0.1 parts of sucralose.

[0055] The preparation process of the product comprises the following steps: S1. dissolving erythritol, sucralose, xanthan gum and the composition in 5 times the amount (based on the amount of the composition) of water at 85° C., and stirring until completely dissolved to obtain a mixture A; S2: adding emblica powder, yeast extract and niacinamide to mixture A, and after the mixture is completely dissolved and slowly added, blood orange juice concentrate, apple juice concentrate, grapefruit juice, red grapefruit juice concentrate, purple carrot juice, potassium sorbate, DL-malic acid, citric acid and sodium citrate are mixed evenly to obtain mixture B; S3 is prepared by adding food flavoring into mixture B and sterilizing the mixture.

[0056] Comparative Example 1 The only difference from Example 3 is that in the preparation process of fish maw oligopeptide, the total amount of fish maw remains unchanged, and the sources of fish maw are different, namely, only Sciaenidae and yellow croaker, with a mass ratio of 3:2; the other conditions are the same.

[0057] Comparative Example 2 The only difference from Example 3 is that in the preparation process of fish maw oligopeptide, the total amount of fish bladder remains unchanged, and the sources of fish bladder are different, namely, only Sciaenidae and silver carp, with a mass ratio of 3:1; the other conditions are the same.

[0058] Comparative Example 3 The only difference from Example 3 is that in the preparation process of fish maw oligopeptide, the total amount of fish maw remains unchanged, the source of fish maw is different, only yellow croaker and silver carp, and the mass ratio of the two is 2:1; the other conditions are the same.

[0059] Comparative Example 4 The only difference from Example 3 is that the enzymatic hydrolysis order is different during the preparation of fish maw oligopeptides, and alkaline protease, bromelain and papain are added in sequence for enzymatic hydrolysis, and the other conditions are the same.

[0060] Comparative Example 5 The only difference from Example 3 is that the enzymatic hydrolysis order is different during the preparation of fish maw oligopeptide, bromelain, alkaline protease and papain are added in sequence for enzymatic hydrolysis, and the other conditions are the same.

[0061] Comparative Example 6 The only difference from Example 3 is that the total mass of collagen tripeptide, bird's nest peptide and fish maw oligopeptide remains unchanged, the mass ratio is different, which is 10:3:0.5, and the other conditions are the same.

[0062] The compositions prepared in Comparative Examples 1 to 6 were used to replace the composition of Example 3 in Application Example 3, respectively, with the dosage unchanged, as Comparative Application Examples 1 to 6.

[0063] Test Example 1 Human food testing Experimental plan: 100 women aged between 35 and 45 were randomly divided into 10 groups, with 10 people in each group. The experimental samples were taken for 30 days. The skin of the subjects was tested with a skin tester before and after taking the samples.

[0064] Experimental samples: Take 25 mL daily according to the formula of each application example or comparative application example. The blank control group does not take any samples, and the diet and daily routine are the same as those of the trial group.

[0065] Test method: Use a clean cotton ball dipped in distilled water to clean the tested skin (1.5 cm horizontally from the outer corner of the eye), dry it for 15 minutes, and then use the Real Bubee RBX-916 skin tester to measure the moisture content of the skin. Use the DJM more me skin tester to measure the skin wrinkles. The test results of each application example and comparative application example are shown in Table 2.

[0066] Table 2 Application examples / comparative application examples test results

[0067] Note: Comparison between the blank control group and application examples 1-3 before and after the test, ** P <0.01; compared with application example 3, # P <0.05,## P <0.01.

[0068] Comparing Application Examples 1-3 with Application Example 3, the fish maw oligopeptides in Application Example 3 use fish bladders from three sources, namely, stonehead cod, yellow croaker and silver carp, while Comparative Application Examples 1-3 only use any two of them, and the total amount of fish maw remains unchanged. As a result, Application Example 3 is significantly superior to Comparative Application Examples 1-3 in improving skin moisture status and wrinkle improvement effects, indicating that fish maw oligopeptides must be prepared using fish bladders from three sources, namely, stonehead cod, yellow croaker and silver carp, in order to achieve excellent results in improving skin moisture status and wrinkle improvement effects.

[0069] Compared with Application Example 3, Application Example 4-Application Example 5 used different enzymatic hydrolysis orders of alkaline protease, papain and bromelain in the preparation process of fish maw oligopeptide. As a result, Application Example 3 was significantly better than Application Example 4-Application Example 5 in improving skin moisture and wrinkle improvement, indicating that a specific enzymatic hydrolysis order must be used to prepare fish maw oligopeptide in order to achieve excellent results in improving skin moisture and wrinkle improvement.

[0070] Comparing Application Example 6 with Application Example 3, the dosage ratio of collagen tripeptide, bird's nest peptide and fish maw oligopeptide is different. As a result, Application Example 3 is significantly better than Comparative Application Example 6 in improving skin moisture status and improving wrinkles, indicating that a specific dosage ratio of collagen tripeptide, bird's nest peptide and fish maw oligopeptide must be used to achieve excellent results in improving skin moisture status and improving wrinkles.

[0071] Test Example 2 Zebrafish assay 1. Sample preparation information The beauty products prepared in each application example and comparative application example are directly absorbed as the original liquid.

[0072] 2. Experimental Animals Zebrafish were raised in fish farming water at 28°C (water quality: conductivity 450-550 μS / cm; pH 6.5-8.5; hardness 50-100 mg / L CaCO3). Broodstock were purchased from Hangzhou Huante Biotechnology Co., Ltd., and fry were obtained by natural mating of broodstock.

[0073] 3. Instruments and consumables Dissecting microscope (SZX7, OLYMPUS, Japan); CCD camera (VertA1, Shanghai Tusen Vision Technology Co., Ltd.); precision electronic balance (CP214, OHAUS, USA); 6-well plate (Zhejiang Bellambo Biotechnology Co., Ltd.).

[0074] 4. Evaluation of hydration efficacy 2 dpf melanin allele mutant Albino strain zebrafish were randomly selected in a 6-well plate, and 30 zebrafish were treated in each well (experimental group). The experimental group was given 12 μL / mL of the beauty and skin care product of the present invention in water solution, and a normal control group and a model control group were set up at the same time, with a capacity of 3 mL per well. Except for the normal control group, the remaining groups were given sodium chloride in water solution to establish a zebrafish dehydration model. After 22 h of treatment at 28°C, 10 zebrafish were randomly selected from each experimental group and placed under a dissecting microscope for photography. The data were analyzed and collected using NIS-Elements D 3.20 advanced image processing software, and the tail area of ​​the zebrafish was analyzed. The statistical analysis results of this indicator were used to evaluate the hydration efficacy of the sample. The statistical processing results were expressed as mean ± SE. SPSS26.0 software was used for statistical analysis, and the results are shown in Table 3.

[0075] Table 3 Zebrafish tail area test results

[0076] Note: Compared with the model control group, * P <0.05,** P <0.01,*** P <0.001; compared with application example 3, # P <0.05.

[0077] Test Example 3 Stability test Place 20 mL of the product of Application Example 1 to Application Example 3 into a centrifuge and centrifuge at 2000 rpm for 10 min. Quickly aspirate the supernatant and measure the absorbance at a wavelength of 750 nm using a UV-visible spectrophotometer. Test the absorbance before and after centrifugation, measure each sample 3 times, and take the average value.

[0078] The stability calculation formula is: Stability W = A 后 / A0*100%; Among them, A 后 W represents the absorbance after centrifugation, and A0 represents the absorbance before centrifugation. The higher the W value, the better the product stability. The results are shown in Table 4.

[0079] Table 4: Stability test results of application examples

[0080] It can be seen from Table 4 that the product of the present invention has good stability. In addition, the product of the present invention has uniform color, no suspended matter, and no stratification.

[0081] The above detailed description is a specific description of one feasible embodiment of the present invention. The embodiment is not intended to limit the patent scope of the present invention. Any equivalent implementation or modification that does not deviate from the present invention should be included in the scope of the technical solution of the present invention.

Claims

1. A composition having a beauty and skin care effect, It is characterized in that The invention comprises the following components in parts by weight: 5-12 parts of fish collagen peptide, 0.1-0.5 parts of type III collagen peptide, 0.1-0.5 parts of skate collagen peptide, 1-3 parts of collagen tripeptide, 0.1-0.5 parts of bird's nest peptide, 0.1-0.3 parts of casein peptide, 0.1-0.5 parts of fish maw oligopeptide, and 0.01-0.05 parts of red and black currant mixed powder; the mass ratio of the collagen tripeptide, bird's nest peptide and fish maw oligopeptide is 10:1-2:1-2; The preparation method of the fish maw oligopeptide comprises the following steps: (1) crushing the fish maw, adding water, and homogenizing to obtain a homogenate; (2) adding enzyme to the homogenate to perform enzymolysis, and then inactivating the enzyme to obtain enzymolysis solution; (3) After centrifuging the enzymatic hydrolysate, the enzymatic hydrolysate is separated by a membrane separation method and dried; The fish bladders in step (1) are sourced from croaker, yellow croaker and silver carp, and the mass ratio of the fish bladders of the three fish is 2-4:1-3:1; In step (2), alkaline protease, papain and bromelain are added in sequence for enzymolysis.

2. The composition according to claim 1, It is characterized in that The mass ratio of the fish maw to water in step (1) is 1:5-10.

3. The composition according to claim 1, It is characterized in that In step (2), the pH of the homogenate is adjusted to 6.0-8.0 before enzymolysis; the amount of the enzyme used is 2-4% of the mass of the homogenate; the temperature of the enzymolysis is 45-55° C., and the time of the enzymolysis is 1.5-3 h.

4. The composition according to claim 1, It is characterized in that The mass ratio of alkaline protease, papain and bromelain in step (2) is 2-4:1-3:4-6; the specific process of enzymatic hydrolysis is as follows: first add alkaline protease for enzymatic hydrolysis for 0.5-1h, then add papain for enzymatic hydrolysis for 0.5-1h, and finally add bromelain for enzymatic hydrolysis for 0.5-1h.

5. The composition according to claim 1, It is characterized in that In step (3), the precipitate is removed by centrifugation to obtain a supernatant for membrane separation; the molecular weight cutoff of the membrane is 3000-5000Da; and the drying method is spray drying.

6. A method for preparing the composition according to any one of claims 1 to 5, It is characterized in that The method comprises the following steps: the components are mixed evenly to obtain the product.

7. A product, It is characterized in that The invention comprises auxiliary materials and the composition described in any one of claims 1 to 5.

8. The product according to claim 7, It is characterized in that The auxiliary materials include at least one of fruit and vegetable juice, emblica powder, yeast extract, nicotinamide, acidulant, thickener, preservative, food flavor and sweetener.

9. The product according to claim 8, It is characterized in that The fruit and vegetable juice includes at least one of blood orange concentrated juice, concentrated apple juice, grapefruit juice, red grapefruit concentrated juice, and purple carrot juice; the thickener includes xanthan gum; the preservative includes potassium sorbate; the sweetener includes at least one of erythritol and sucralose; and the acidulant includes at least one of DL-malic acid, citric acid, and sodium citrate.

10. The product according to claim 9, It is characterized in that The invention comprises, by weight, 30-50 parts of the composition, 1-5 parts of blood orange concentrated juice, 5-10 parts of concentrated apple juice, 1-5 parts of grapefruit juice, 0.5-2 parts of red grapefruit concentrated juice, 0.5-2 parts of purple carrot juice, 0.1-1 parts of emblica powder, 0.1-1 parts of yeast extract, 0.01-0.015 parts of nicotinamide, 0.5-2 parts of DL-malic acid, 0.5-2 parts of citric acid, 0.2-2 parts of sodium citrate, 0.2-1 parts of xanthan gum, 0.01-0.1 parts of potassium sorbate, 0.05-0.5 parts of food flavor, 1-10 parts of erythritol and 0.05-0.2 parts of sucralose.

11. Use of the composition according to any one of claims 1 to 5 in preparing products having beauty and skin care effects.

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