Preparation method of reconstituted tobacco based on biotechnology

Abstract

The invention discloses a preparation method of reconstituted tobacco based on biotechnology, which comprises the following steps: (1) crushing tobacco, tobacco powder or tobacco stem to obtain tobacco powder; (2) after inactivating the tobacco powder, firstly adding water, then adding microorganisms or enzymes, and carrying out biotechnological treatment under certain conditions; and (3) adding a smoke agent, an adhesive, water and cellulose into the tobacco powder treated by the biotechnology, and uniformly mixing to prepare the reconstituted tobacco. In the preparation process of the reconstituted tobacco, the technological characteristics of the reconstituted tobacco processing process can be fully combined, the tobacco powder is treated through the biotechnology, internal chemical components are promoted to be converted and harmonized, solid-liquid separation, coating and other technologies are not needed, and technology application and popularization are facilitated. The prepared reconstituted tobacco is applied to tobacco products, compared with reconstituted tobacco prepared in the prior art, the sensory quality is obviously improved, the aroma quality and the aroma amount are obviously improved, the tobacco characteristic aroma is more prominent, and irritation and offensive odor are obviously reduced.

Description

Technical Field

[0001] The invention relates to the technical field of tobacco, and in particular to a method for preparing reconstituted tobacco leaves based on biotechnology. Background Art

[0002] Reconstituted tobacco refers to a regenerated product made from tobacco stems, tobacco dust, and broken tobacco flakes in sheet or filament form. It is used as a filler for cigarettes, which can save tobacco raw materials to the maximum extent and effectively reduce the cost of cigarettes. Reconstituted tobacco has the characteristics of high filling value, good combustibility, stable and adjustable physical and chemical indicators, and low release of harmful substances. It is used in huge amounts in cigarette products and has a significant impact on the sensory quality of cigarette products. However, at present, reconstituted tobacco still generally has defects such as weak characteristic aroma style, insufficient aroma, and poor coordination. Therefore, improving the sensory quality of reconstituted tobacco is a common problem that needs to be solved urgently by various cigarette industrial enterprises.

[0003] At present, many research results have been achieved by using biotechnology to improve the quality of reconstituted tobacco leaves, promote the transformation and coordination of chemical components inside tobacco leaves, increase the types and contents of substances that are beneficial to sensory quality, and reduce the types and contents of substances that are not conducive to sensory quality. Patent CN201611178971 adds Bacillus cereus to the soaked tobacco raw material, separates after the reaction, obtains an extract and insoluble matter, vacuum rotary evaporation and concentration of the extract to obtain a concentrate, and the insoluble matter is made into a substrate by papermaking technology, and the concentrate is coated on the substrate and dried to obtain reconstituted tobacco leaves. Maillard reaction occurs during the fermentation process, producing aroma. Patent CN201610781986 uses Bacillus methylotrophicus (VJ4-1), Penicillium citrinum ferulic acid esterase, xylanase, glucose oxidase, catalase, and phenol oxidase complex enzymes to treat the aqueous solution of the reconstituted tobacco raw materials during the processing of reconstituted tobacco leaves. The bioconversion liquid is separated from the liquid residue by disc snail centrifugation to obtain a concentrated liquid, which is sprayed onto a tobacco substrate and rolled into reconstituted tobacco leaves with improved aroma quality. The produced reconstituted tobacco leaves have reduced irritation, reduced green odor, and have a beautiful aroma with a slight milk aroma, thereby improving the sensory quality of the reconstituted tobacco leaves. Patent CN201610782009 uses Enterobacter mori VL4-3, butyric acid bacteria ferulic acid esterase, lignin peroxidase Lip and manganese peroxidase MnP to treat the aqueous solution of the reconstituted tobacco raw material during the processing of reconstituted tobacco leaves. The bioconversion liquid is centrifuged by a disc snail to separate the liquid residue to obtain a concentrated liquid, which is sprayed on the tobacco substrate to roll into reconstituted tobacco leaves with improved aroma quality. Patent CN201310142833 uses a composite amylase, endoglucanase, exoglucanase, and β-glucosidase compound preparation to add to the tobacco raw material in the extraction section, and separates the tobacco soluble matter and the tobacco insoluble matter from the tobacco raw material after biological enzyme degradation. After the concentrated liquid is prepared into a dipping liquid, it is coated on the paper base of the reconstituted tobacco leaves at a dipping amount of 38%, thereby improving the smoking quality of the reconstituted tobacco leaves made by the papermaking method.

[0004] However, the above technologies require processes such as solid-liquid separation and coating, and the technology promotion and application are difficult. Therefore, developing a preparation method for reconstituted tobacco leaves based on biotechnology that does not require processes such as solid-liquid separation and coating is a research direction with great innovation and application prospects. Summary of the invention

[0005] The technical problem to be solved by the present invention is to provide a method for preparing reconstituted tobacco leaves based on biotechnology, aiming to improve the quality of reconstituted tobacco leaves through biotechnology so that their application in tobacco products is more extensive and effective.

[0006] The technical problem to be solved by the present invention is achieved through the following technical solutions:

[0007] A method for preparing reconstituted tobacco leaves based on biotechnology comprises the following steps:

[0008] (1) crushing tobacco leaves, tobacco dust or tobacco stems to obtain tobacco powder;

[0009] (2) After the tobacco powder is inactivated, water is first added, and then microorganisms or enzymes are added, and biotechnology treatment is performed under certain conditions;

[0010] (3) adding a smoke generating agent, a binder, water and cellulose to the tobacco powder treated by biotechnology and mixing them to prepare reconstituted tobacco leaves, wherein the mass ratio of the smoke generating agent to the tobacco powder is 0.1:1 to 0.3:1, the mass ratio of the binder to the tobacco powder is 0.001:1 to 0.05:1, and the mass ratio of the cellulose to the tobacco powder is 10:1 to 10:1. -3 :1~10 -1 :1.

[0011] Preferably, in step (1), the particle size of the smoke powder is 50 to 400 meshes.

[0012] Preferably, step (2) is specifically:

[0013] (21) First, inactivate the smoke powder at 90-130°C for 0.1-1 h;

[0014] (22) adding water to the tobacco powder, wherein the mass ratio of water to tobacco powder is 1:1 to 6:1;

[0015] (23) Then, microorganisms or enzymes are added for biotechnology treatment. The mass ratio of microorganisms or enzymes to tobacco powder is 10 -4 :1~10 -1 :1;

[0016] (24) The biotechnology treatment temperature is 30-50°C and the treatment time is 0.1-72h.

[0017] Preferably, in step (2), the microorganism is one or more of a flavor enhancing microorganism, an impurity removing microorganism, a thorn reducing microorganism, and a harm reducing microorganism.

[0018] Preferably, in step (2), the microorganism is Shinella sp. FLN14, and the preservation unit is China Center for Type Culture Collection, with the preservation number being CCTCC NO: M 2022845.

[0019] Preferably, in step (2), the enzyme is one or more of carotenoid degrading enzyme, protease, peptidase, amylase, cellulase, hemicellulase, pectinase, lignin degrading enzyme, nicotine degrading enzyme, saccharifying enzyme, oxidoreductase, and dextrin degrading enzyme.

[0020] Preferably, in step (3), the smoke generating agent is one or more of glycerol, 1,3-propylene glycol, 1,2-propylene glycol, sorbitol, isosorbide, xylitol, and erythritol, and the mass ratio of the smoke generating agent to the smoke powder is 0.1:1 to 0.3:1.

[0021] Preferably, in step (3), the binder is one or more of sodium carboxymethyl cellulose, sodium carboxymethyl starch, gellan gum, agar powder, carrageenan, and gum arabic, and the mass ratio of the binder to the tobacco powder is (0.001-0.05):1.

[0022] Preferably, in step (3), the cellulose is one or more of wood pulp fiber, hemp pulp fiber, straw pulp fiber, and nanocellulose, and the mass ratio of the cellulose to the tobacco powder is 10 -3 :1~10 -1 :1.

[0023] A heated cigarette comprising the reconstituted tobacco leaves prepared by the above-mentioned preparation method.

[0024] The invention discloses an application of Shinella sp. FLN14 in reconstituted tobacco leaves, characterized in that the strain is preserved in China Center for Type Culture Collection and its preservation number is CCTCC NO: M2022845.

[0025] The above technical solution of the present invention has the following beneficial effects:

[0026] (1) This method first uses microorganisms or enzymes to treat tobacco powder, and then adds smoke-generating agents, adhesives, water and cellulose to make reconstituted tobacco. This is the first report of this method, which does not require solid-liquid separation, concentration, coating and other processes. It is suitable for the processing technology of roller-pressed and thick pulp-processed reconstituted tobacco, and is conducive to the promotion and application of the technology.

[0027] (2) This method uses microorganisms or enzymes to treat tobacco powder. The biological action process is sufficient and uniform, the material uniformity is good, and the sensory quality of the prepared slices is uniform and stable. BRIEF DESCRIPTION OF THE DRAWINGS

[0028] The accompanying drawings, which are incorporated in and constitute a part of the specification, illustrate embodiments of the invention and, together with the description, serve to explain the principles of the invention.

[0029] Figure 1 It is the mixed material before coating.

[0030] Figure 2 The process of coating materials to form reconstituted tobacco leaves.

[0031] Figure 3 The obtained reconstituted tobacco leaf samples.

[0032] Figure 4 The prepared reconstituted tobacco leaf samples were cut into shreds. DETAILED DESCRIPTION

[0033] Various exemplary embodiments of the present invention will now be described in detail with reference to the accompanying drawings. It should be noted that the relative arrangement of components and steps, numerical expressions and numerical values ​​set forth in these embodiments do not limit the scope of the present invention unless otherwise specifically stated.

[0034] Unless otherwise specified, the following experimental materials were commercially available or obtained through other commercial channels.

[0035] Strain Information:

[0036] Shinella sp.FLN14, the preservation number of this strain is CCTCC NO: M2022845, the preservation date is June 9, 2022, the preservation unit is China Center for Type Culture Collection, and the preservation address is Wuhan University, China.

[0037] Example 1

[0038] A method for preparing reconstituted tobacco leaves based on biotechnology comprises the following steps:

[0039] Take 50g of tobacco leaves, grind them with a ball mill, pass them through a 50-mesh sieve, put them into a 1000ml conical flask, add 50g of water into the flask, mix them thoroughly, and sterilize them at 120℃ for 0.2h.

[0040] 10% of the tobacco powder mass was inoculated with the bacterium Bacillus shenkelii FLN14 and treated at 40°C for 48 hours.

[0041] After the treatment, the material was transferred out of the triangular flask, and 5g of glycerol, 0.05g of wood pulp fiber, and 0.05g of sodium carboxymethyl cellulose were added in sequence to obtain a mixed material (such as Figure 1 ).

[0042] The material is made into reconstituted tobacco leaves (such as Figure 2 , Figure 3 ), cut the reconstituted tobacco leaves into shredded tobacco using a shredder (such as Figure 4 ) is made into heated cigarette products.

[0043] The preparation process of the control sample is the same as above, except that Bacillus shenkelii FLN14 is not inoculated, water of the same mass as the bacterial agent is added, and subsequent treatment is performed in the same manner. The sensory quality of the reconstituted tobacco leaves was evaluated, and the scoring results are shown in Table 1.

[0044] Example 2

[0045] A method for preparing reconstituted tobacco leaves based on biotechnology comprises the following steps:

[0046] Take 50g of tobacco powder, grind it with a ball mill, pass it through a 100-mesh sieve, put it into a 1000ml conical flask, add 50g of water into the flask and mix it thoroughly, and sterilize it at 90℃ for 1.0h.

[0047] Inoculate the tobacco powder with 1% of its mass into flavor-enhancing microorganisms and treat at 35°C for 60 hours.

[0048] After the treatment, the material was transferred out of the conical flask, and 5 g of 1,2-propylene glycol, 5 g of nanocellulose, and 0.25 g of agar powder were added in sequence to obtain a mixed material.

[0049] The material is made into reconstituted tobacco leaves by using a coating machine, and the reconstituted tobacco leaves are cut into shredded tobacco by using a shredder to make heated cigarette products.

[0050] The preparation process of the control sample is the same as above, except that the aroma-enhancing microorganisms are not inoculated, water of the same mass as the microbial agent is added, and subsequent treatment is performed in the same manner. The sensory quality of the reconstituted tobacco leaves was evaluated, and the scoring results are shown in Table 1.

[0051] Example 3

[0052] A method for preparing reconstituted tobacco leaves based on biotechnology comprises the following steps:

[0053] Take 50g of tobacco stems, grind them with a ball mill, pass them through a 200-mesh sieve, put them into a 1000ml conical flask, add 150g of water into the flask, mix them thoroughly, and sterilize them at 130℃ for 0.1h.

[0054] Lignin degrading enzyme was added at 0.1% of the mass of smoke powder and treated at 30°C for 72h.

[0055] After the treatment, the material was transferred out of the triangular flask, and 10 g of sorbitol, 0.25 g of hemp pulp fiber, and 1.0 g of carrageenan were added in sequence to obtain a mixed material.

[0056] The material is made into reconstituted tobacco leaves by using a coating machine, and the reconstituted tobacco leaves are cut into shredded tobacco by using a shredder to make heated cigarette products.

[0057] The preparation process of the control sample was the same as above, except that no lignin degrading enzyme was added, and the subsequent treatment was carried out in the same manner. The sensory quality of the reconstituted tobacco leaves was evaluated, and the scoring results are shown in Table 1.

[0058] Example 4

[0059] A method for preparing reconstituted tobacco leaves based on biotechnology comprises the following steps:

[0060] Take 50g of tobacco leaves, grind them with a ball mill, pass them through a 300-mesh sieve, put them into a 1000ml conical flask, add 250g of water into the flask, mix them thoroughly, and sterilize them at 100℃ for 0.3h.

[0061] Carotenoid-degrading enzyme was added at 0.1% of the tobacco powder mass and treated at 45°C for 36 hours.

[0062] After the treatment, the material was transferred out of the triangular flask, and 10 g of xylitol, 0.5 g of straw pulp fiber, and 0.15 g of gum arabic were added in sequence to obtain a mixed material.

[0063] The material is made into reconstituted tobacco leaves by using a coating machine, and the reconstituted tobacco leaves are cut into shredded tobacco by using a shredder to make heated cigarette products.

[0064] The preparation process of the control sample was the same as above, except that no carotenoid degrading enzyme was added, and the subsequent treatment was carried out in the same manner. The sensory quality of the reconstituted tobacco leaves was evaluated, and the scoring results are shown in Table 1.

[0065] Example 5

[0066] A method for preparing reconstituted tobacco leaves based on biotechnology comprises the following steps:

[0067] Take 50g of tobacco stems, grind them with a ball mill, pass them through a 400-mesh sieve, put them into a 1000ml conical flask, add 300g of water into the flask, mix them thoroughly, and sterilize them at 110℃ for 0.5h.

[0068] Cellulase was added at 0.3% of the tobacco powder mass and treated at 50°C for 0.1h.

[0069] After the treatment, the material was transferred out of the triangular flask, and 15 g of erythritol, 5 g of nanocellulose, and 2.5 g of sodium carboxymethyl starch were added in sequence to obtain a mixed material.

[0070] The material is made into reconstituted tobacco leaves by using a coating machine, and the reconstituted tobacco leaves are cut into shredded tobacco by using a shredder to make heated cigarette products.

[0071] The preparation process of the control sample was the same as above, except that no cellulase was added, and the subsequent treatment was carried out in the same manner. The sensory quality of the reconstituted tobacco leaves was evaluated, and the scoring results are shown in Table 1.

[0072] Table 1 Analysis of aroma components in aerosol of heated cigarette smoke made from reconstituted tobacco leaves in different embodiments

[0073]

[0074]

[0075] The smoke aerosol aroma components of the heated cigarettes prepared in Examples 1 to 5 were analyzed, and the results are shown in Table 1. It can be seen that compared with the control sample, after the tobacco powder was treated with biotechnology, the contents of ketones, aldehydes, alcohols, phenols, esters and nitrogen-containing compounds were increased to varying degrees, which had a positive effect on improving the sensory quality of heated cigarettes. The degree of increase in the content of the corresponding smoke aerosol aroma components was different when different biotechnologies were used.

[0076] Table 2 Sensory evaluation results of reconstituted tobacco leaves prepared in different embodiments

[0077] serial number Flue gas temperature Smoke volume fragrance Momentum Irritant Comfort Uniformity Total score Example 1 12.5 12.5 23.6 7.9 8.3 12.6 8.2 85.6 Example 1 comparison 12.6 12.5 21.7 7.9 8.6 11.9 8.3 83.5 Example 2 12.4 12.5 23.6 7.8 8.4 12.7 8.3 85.7 Example 2 comparison 12.4 12.6 21.4 7.9 8.5 11.9 8.2 82.9 Example 3 12.5 12.6 21.4 7.8 7.9 11.3 8.3 81.8 Example 3 Comparison 12.5 12.5 20.3 7.9 7.1 10.8 8.2 79.3 Example 4 12.5 12.6 24.0 7.8 8.4 12.5 8.3 86.1 Example 4 Control 12.4 12.6 21.6 7.9 8.7 11.8 8.3 83.3 Example 5 12.4 12.5 21.7 7.9 7.8 11.3 8.3 81.9 Example 5 Comparison 12.4 12.6 20.4 7.9 7.2 10.7 8.2 79.4

[0078] From the results shown in Table 2, it can be seen that compared with the control sample, the three indicators of flavor, irritation and comfort of the five groups of heated cigarettes prepared by Examples 1 to 5 are significantly improved. From the comparison results of different embodiments, it can be seen that the degree of improvement of the above indicators is different when different biological treatment methods are applied; in addition, there is no obvious change in the four indicators of smoke temperature, smoke volume, strength and uniformity.

[0079] Although the present invention has been disclosed as above by the embodiments, it is not intended to limit the present invention. Any person skilled in the art may make various choices and modifications without departing from the spirit and scope of the present invention. Therefore, the protection scope of the present invention is limited by the claims and their equivalents.

Claims

1. A method for preparing reconstituted tobacco leaves based on biotechnology, It is characterized in that The following steps are involved: (1) crushing tobacco leaves, tobacco dust or tobacco stems to obtain tobacco powder; (2) After the tobacco powder is inactivated, water is first added, and then microorganisms or enzymes are added, and biotechnology treatment is performed under certain conditions; (3) adding a smoke generating agent, a binder, water and cellulose to the tobacco powder treated by biotechnology and mixing them to prepare reconstituted tobacco leaves, wherein the mass ratio of the smoke generating agent to the tobacco powder is 0.1:1 to 0.3:1, the mass ratio of the binder to the tobacco powder is 0.001:1 to 0.05:1, and the mass ratio of the cellulose to the tobacco powder is 10:1 to 10:

1. -3 :1~10 -1 :

1.

2. The method for preparing reconstituted tobacco leaves based on biotechnology according to claim 1, It is characterized in that In step (1), in step (1), the particle size of the smoke powder is 50 to 400 meshes.

3. The method for preparing reconstituted tobacco leaves based on biotechnology according to claim 1, It is characterized in that Step (2) is specifically as follows: (21) First, inactivate the smoke powder at 90-130°C for 0.1-1 h; (22) adding water to the tobacco powder, wherein the mass ratio of water to tobacco powder is 1:1 to 6:1; (23) Then, microorganisms or enzymes are added for biotechnology treatment. The mass ratio of microorganisms or enzymes to tobacco powder is 10 -4 :1~10 -1 :1; (24) The biotechnology treatment temperature is 30-50°C and the treatment time is 0.1-72h.

4. The method for preparing reconstituted tobacco leaves based on biotechnology according to claim 3, It is characterized in that In step (2), the microorganism is one or more of a flavor-enhancing microorganism, an impurity-removing microorganism, a thorn-reducing microorganism, and a harm-reducing microorganism; the enzyme is one or more of a carotenoid-degrading enzyme, a protease, a peptidase, an amylase, a cellulase, a hemicellulase, a pectinase, a lignin-degrading enzyme, a nicotine-degrading enzyme, a saccharifying enzyme, an oxidoreductase, and a dextrin-degrading enzyme.

5. The method for preparing reconstituted tobacco leaves based on biotechnology according to claim 3, It is characterized in that In step (2), the microorganism is Shinella sp. FLN14, which is deposited in China Center for Type Culture Collection with a deposit number of CCTCC NO: M 2022845.

6. The method for preparing reconstituted tobacco leaves based on biotechnology according to claim 1, It is characterized in that In step (3), the smoke generating agent is one or more of glycerol, 1,3-propylene glycol, 1,2-propylene glycol, sorbitol, isosorbide, xylitol, and erythritol.

7. The method for preparing reconstituted tobacco leaves based on biotechnology according to claim 1, It is characterized in that In step (3), the binder is one or more of sodium carboxymethyl cellulose, sodium carboxymethyl starch, gellan gum, agar powder, carrageenan, and gum arabic.

8. The method for preparing reconstituted tobacco leaves based on biotechnology according to claim 1, It is characterized in that In step (3), the cellulose is one or more of wood pulp fiber, hemp pulp fiber, straw pulp fiber, and nanocellulose.

9. A heated cigarette, It is characterized in that The invention relates to reconstituted tobacco leaves prepared by the preparation method according to any one of claims 1 to 9.

10. Application of Shinella sp. FLN14 in reconstituted tobacco leaves, It is characterized in that The strain is preserved in China Center for Type Culture Collection, and the preservation number is CCTCC NO: M 2022845.

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