Restrained heat stress experiment device

Abstract

The invention discloses a constraint heat stress experiment device, and relates to the technical field of mouse experiment equipment, the constraint heat stress experiment device comprises an experiment box, a placing seat is arranged in the experiment box, a cage body is arranged in the placing seat, a heating assembly is arranged in the experiment box, and a vibration assembly for driving the cage body to vibrate is arranged in the experiment box. The mouse is bound through the cage body, the cage body is placed in the placement seat, the testis part of the mouse is subjected to heat stress through the heating assembly arranged in the experiment box, and in the heat stress process, the vibration assembly works to enable the cage body to vibrate and generate noise, so that adaptive sleep of the mouse in the heat stress process is prevented; therefore, mouse bound heat stress experiment data are more accurate.

Description

Technical Field

[0001] The invention relates to the technical field of experimental equipment, and in particular to a restrained heat stress experimental device. Background Art

[0002] Studies have found that excessive daily stress and prolonged sitting can cause testicular temperature to rise, leading to related diseases in various aspects of multiple systems such as metabolism, nerves, reproduction, immunity, and cardiovascular and cerebrovascular systems. Excessive stress caused by study, examinations, work, etc., and long periods of sitting can lead to a decline in various functions of the body, greatly increasing the risk of disease. Restraint stress + heat stress is a non-damaging stimulus that can better simulate a series of life conditions in human life, such as "uncontrollable" crowding, "unfaceable" frustration, and long-term sitting. That is, it is similar to the process of related diseases caused by stress in humans. Therefore, restraint stress + heat stress is a good stress model. Studies have found that the restraint stress model of experimental animals can better simulate the stress response caused by daily human stress, and local testicular heat stress can also better simulate the state of sitting for a long time.

[0003] There are two closest related technologies for existing restraint stress and related heat stress devices: one is to use a 50mL centrifuge tube to put the experimental animal in it, conduct a restraint experiment, and then conduct heat stress through the poor heat dissipation of the centrifuge tube. The second is to tie up the limbs of the experimental animal to achieve the restraint effect, and then put it into a constant temperature box for heat stress after tying. The first restraint device places the experimental animal in a centrifuge tube with a closed space. Although the device uses body temperature and its poor heat dissipation to conduct heat stress, the effect of local testicular heat stress is not significant; the second restraint method is not easy to operate for smaller experimental animals, and it is not easy to tie up their limbs smoothly. If the experimental animal struggles, this device is very likely to cause damage to the experimental animal, and the local heat stress effect is not significant, and it cannot simulate the situation of sitting for a long time under pressure very well; both devices are relatively complex and difficult to operate. Summary of the invention

[0004] The purpose of the present invention is to provide a restraint heat stress experimental device with more accurate experimental data. The present invention restrains the experimental subject in a cage, applies heat stress to the experimental subject through a heating component, and vibrates the experimental subject in the cage through a vibration component, thereby making the heat stress experimental data of the restraint experimental subject more accurate.

[0005] To achieve the above-mentioned purpose, the technical solution adopted by the present invention to solve its technical problem is: a restrained heat stress experimental device, including an experimental box, a placement seat is provided in the experimental box, a cage is provided in the placement seat, a heating component is provided in the experimental box, and a vibration component for driving the cage to vibrate is provided in the experimental box.

[0006] The experimental box includes an upper box body, a lower box body and an upper cover. A side panel is provided between the upper box body and the lower box body. The side panel is connected to the upper box body and the lower box body through a second bolt. The upper cover is connected to the upper box body through a first bolt. The upper box body is provided with a placement hole for the cage body to move.

[0007] The cage body is provided with a slide column, the placement seat is provided with a slide groove matched with the slide column, the cage body is provided with a cover, one end of the cover is hinged to the cage body, the other end of the cover is connected to the cage body through a third bolt, and an empty groove is provided on the lower side of the placement seat.

[0008] A partition cage is arranged in the cage body, a liquid injection port is arranged on the cover, a heating medium is arranged in the placement seat, a third grip ring is arranged on the cover, and air holes are arranged on the upper box body, the prevention seat and the cage body.

[0009] The heating assembly includes a heating plate and an electric heating wire, the heating plate is connected to the upper box body, the electric heating wire is connected to the heating plate, the electric heating wire is arranged at the lower side of the placement seat, and a temperature detector is arranged in the placement seat.

[0010] Slide plates are provided at both ends of the heating plate, guide grooves for the slide plates to slide are provided on the upper box body, a limit plate is provided on one end of the slide plate extending outside the upper box body, and a first grip ring is provided on the limit plate.

[0011] The vibration mechanism includes a motor, a first gear, a second gear, a connecting shaft, a third gear, a rotating shaft, a cam and a moving plate. The motor is connected to the lower box body, the power output shaft of the motor is connected to the first gear, the connecting shaft is connected to the lower box body, the connecting shaft is connected to the second gear, the first gear is meshed with the second gear, the rotating shaft is connected to the lower box body, the rotating shaft is connected to the third gear, the third gear is meshed with the second gear, the rotating shaft is connected to the cam, one end of the moving plate is in contact with the moving seat, and the other end of the moving plate is in contact with the cam.

[0012] A first guide cylinder is arranged at one end of the placement seat close to the upper cover, a guide column is arranged on the upper cover, and the first guide cylinder is sleeved on the guide column.

[0013] A second guide cylinder is provided on the side of the movable plate close to the placement seat, a third guide cylinder is provided on the placement seat, the second guide cylinder is sleeved on the third guide cylinder, a spring is provided between the second guide cylinder and the third guide cylinder, a contact block is provided on the end of the movable plate close to the cam, and an abutting surface contacting the cam is provided on the contact block.

[0014] A card seat is arranged in the placement hole, a card plate is arranged on a side of the card seat close to the cage body, the card seat contacts the sealing cover, an avoidance groove is arranged on the card seat, a second grip ring is arranged on the card seat, and a control panel is arranged on the upper cover.

[0015] The beneficial effects of the present invention are:

[0016] The mice are restrained by a cage, which is placed in a placement seat. The testicles of the mice are subjected to heat stress by a heating component arranged in an experimental box. During the heat stress process, the vibration component works to vibrate the cage and generate noise, thereby preventing the mice from experiencing adaptive sleep during the heat stress process, thereby making the mouse restraint heat stress experimental data more accurate.

[0017] Multiple cages are arranged in the experimental box to conduct experiments on multiple mice. The entire device has a simple structure, is easy to manufacture, and has a low manufacturing cost. The moving plate in the experimental box is moved up and down by a motor, a first gear, a second gear, a third gear, a rotating shaft, and a cam, and then the moving plate drives the placement seat to move up and down, thereby achieving vibration of the mice in the cage.

[0018] A first guide cylinder, a guide column, a second guide cylinder and a third guide cylinder are respectively provided on both sides of the placement seat to guide the movement of the placement, and a spring is provided between the second guide cylinder and the third guide cylinder. The elasticity of the spring realizes elastic contact between the moving plate and the placement seat, thereby preventing the moving plate and the placement seat from being damaged during vibration.

[0019] The card seat is placed in the placement hole. At this time, the card plate on the card seat supports the cover, thereby supporting the cage body and the placement seat. Then the cam rotates so that the extrusion spring cannot vibrate the placement seat. The experimenter can fix the cage body as needed, and the cage body is fixed quickly and conveniently. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 It is a schematic structural diagram of the restrained heat stress experimental device of the present invention.

[0021] Figure 2 for Figure 1 Top view of the .

[0022] Figure 3 for Figure 1 Front view of .

[0023] Figure 4 for Figure 3 Schematic diagram of the BB cross-section structure.

[0024] Figure 5 for Figure 1 Schematic diagram of the internal structure of the lower box.

[0025] Figure 6 for Figure 5 Top view of the .

[0026] Figure 7 for Figure 1 Schematic diagram of the connection structure between the placement seat and the cage body.

[0027] Figure 8 for Figure 7 Front view of .

[0028] Fig. 9 for Figure 8 Schematic diagram of the cross-sectional structure.

[0029] Fig.10 for Figure 1 Schematic diagram of the cage structure.

[0030] Fig.11 for Figure 1 Schematic diagram of the connection structure of the moving plate, the contact block and the second guide cylinder.

[0031] Fig.12 for Figure 1 Schematic diagram of the connection structure of the holder, the card plate and the second handle.

[0032] Fig.13 for Fig.12 Another perspective structural diagram of .

[0033] Fig.14 for Figure 1 Schematic diagram of the heating component structure.

[0034] Fig.15 for Fig.14 Top view of the .

[0035] Fig.16 for Figure 1 Schematic diagram of the upper box structure.

[0036] Fig.17 for Figure 1 Schematic diagram of the placement seat structure.

[0037] Fig.18 The bar graph shows the effects of restraint combined with heat stress on testis weight in mice.

[0038] Fig.19 This is a bar graph showing the effects of restraint combined with heat stress on testicular organ indexes in mice.

[0039] Fig. 20 This is an optical micrograph showing the effects of restraint combined with heat stress on the testicular tissue structure of mice.

[0040] Fig.21 This is a bar graph showing the effects of restraint combined with heat stress on the area of ​​seminiferous tubules in mouse testes.

[0041] Fig. 22 Optical micrographs showing the effects of restraint combined with heat stress on the structure and cellular composition of the epididymal capu and cauda of mice.

[0042] Fig.23 The bar graph shows the effects of restraint combined with heat stress on sperm concentration in the cauda epididymis of mice.

[0043] Fig.24 This is a bar graph showing the effects of restraint combined with heat stress on the sperm deformity rate in the cauda epididymis of mice.

[0044] Fig.25 This is an optical microscopic image of the effect of restraint combined with heat stress on the number of germ cells in the seminiferous tubules of mice.

[0045] Fig.26 Optical micrographs showing the effects of restraint combined with heat stress on meiotic cells in mouse seminiferous tubules.

[0046] In the attached figure: 100-experimental box, 1-upper box, 2-lower box, 3-upper cover, 4-first bolt, 5-side plate, 6-second bolt, 7-placement seat, 8-cage, 9-placement hole, 10-cover, 11-third bolt, 12-sliding column, 13-sliding groove, 14-first guide cylinder, 15-guide column, 16-motor, 17-first gear, 18-second gear, 19-connecting shaft, 20-third gear, 21-rotating shaft, 22-cam, 23-moving Plate, 24-contact plate, 25-abutment surface, 26-second guide cylinder, 27-third guide cylinder, 28-spring, 29-heating plate, 30-electric heating wire, 31-temperature detector, 32-slide plate, 33-limiting plate, 34-first grip ring, 35-holder, 36-holder, 37-second grip ring, 38-avoidance groove, 39-control panel, 40-empty groove, 41-guide groove, 42-partition cage, 43-liquid injection port, 44-heating medium, 45-third grip ring. DETAILED DESCRIPTION

[0047] In order to make the purpose, technical solution and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not intended to limit the present invention.

[0048] The specific implementation of the present invention is described in detail below in conjunction with specific embodiments.

[0049] like Figure 1-17As shown, the restraint heat stress experimental device includes an experimental box 100, a placement seat 7 is provided in the experimental box 100, a cage 8 is provided in the placement seat 7, a heating component is provided in the experimental box 100, and a vibration component for driving the cage 8 to vibrate is provided in the test box 100. In this embodiment, the cage 8 is in the shape of an elongated strip, and the mouse is placed in the cage 8 to maintain the same posture to simulate the restraint state. Two rows of placement seats 7 are provided in the experimental box 100, and each row of placement seats 7 includes three placement seats 7 arranged in parallel. Multiple cages 8 are used to realize simultaneous experiments on multiple mice in one device. The entire device has a simple structure, is easy to manufacture, and reduces the manufacturing cost.

[0050] The mice to be tested are placed in a cage 8, and the mice are restrained by the cage 8. The cage 8 is placed in a placement seat 7, and the testicles of the mice are subjected to heat stress by a heating component arranged in an experimental box 100. During the heat stress process, the vibration component works to make the cage 8 vibrate and generate noise, thereby preventing the mice from experiencing adaptive sleep during the heat stress process, thereby making the mouse restraint heat stress experiment data more accurate.

[0051] Reference Figure 1-3 The experimental box 100 includes an upper box body 1, a lower box body 2 and an upper cover 3. A side plate 5 is provided between the upper box body 1 and the lower box body 2. The side plate 5 is connected to the upper box body 1 and the lower box body 2 through a second bolt 6. The side plate 5 and the second bolt 6 are used to realize convenient disassembly and assembly between the upper box body 1 and the lower box body 2. The upper cover 3 is connected to the upper box body 1 through a first bolt 4. The first bolt 4 is used to realize a quick connection between the upper cover 3 and the upper box body 1. The upper box body 1 is provided with a placement hole 9 for moving a cage body 8. The cage body 8 is installed in the placement seat 8 through the placement hole 9.

[0052] In this embodiment, there are four side panels 5, which are symmetrically arranged on both sides of the experimental box 100. Each side panel 5 is connected to the upper box body 1 through two second bolts 6, and each side panel 5 is connected to the lower box body 2 through two second bolts 6. The side panel 5 is provided with a through hole for the second bolt 6 to pass through, and the upper box body 1 and the lower box body 2 are provided with threaded holes that cooperate with the second bolts 6.

[0053] Reference Fig.18In another embodiment, a separation cage 42 is provided in the cage body 8 in the placement seat 7, and mice are placed in the separation cage 42, and a liquid injection port 43 is provided on the cover 10 at the side end of the placement seat 7, and a heating medium 44 is injected into the placement seat 7 through the liquid injection port 43, so that when the heating component on the lower side of the placement seat 7 is heated, the mice are heated by the heating medium 44, thereby ensuring stable heating of the mouse testicles, thereby ensuring stable thermal stress of the mice, and making the experimental data more accurate. Specifically, three separation cages 42 are provided in the cage body 8, and the sealing cap 10 is provided with a liquid injection port 43. The heating medium 44 is injected into the placement seat 7 through the liquid injection port 43, so that when the heating component on the lower side of the placement seat 7 is heated, the mouse is heated by the heating medium 44, thereby ensuring stable heating of the mouse testicles, thereby ensuring stable thermal stress of the mouse, and making the experimental data more accurate. A sealing gasket is provided on 10 to prevent the heating medium 44 from flowing out of the placement seat 7. Specifically, the heating medium is water. In this embodiment, a temperature sensor is arranged in the placement seat 7 to detect the heating medium 44 in the placement seat 7, thereby ensuring that the temperature of the heating medium is within the experimental requirements, and a third grip ring 45 is provided on the cover 10, so that the user can hold the third grip ring 45 to move the cover 10 and then open the cover 10. Air holes are provided on the upper box body 1, the prevention seat 7 and the cage body 8, thereby ensuring the stable survival of mice in the experimental box 100.

[0054] Reference Figure 7-17 The cage body 8 is provided with a slide post 12, and the placement seat 7 is provided with a slide groove 13 that cooperates with the slide post 12. The cage body 8 is installed in the placement seat 7 through the cooperation of the slide post 12 and the slide groove 13. In this embodiment, the number of the slide post 12 and the slide groove 13 is two, and a cover 10 is provided on the cage body 8. One end of the cover 10 is hinged to the cage body 8, and the other end of the cover 10 is connected to the cage body 8 through a third bolt 11. The third bolt 11 is rotated to allow the cover 10 to rotate freely at the side end of the cage body 8. At this time, the mouse is placed in the cage body 8, the cover 10 is turned over, and the cover 10 is fixed by rotating the third bolt 11, thereby realizing the restraint of the mouse in the cage body 8. An empty groove 40 is provided on the lower side of the placement seat 7. The setting of the empty groove 40 facilitates the heating component to heat the cage body 8 in the placement seat 7.

[0055] Reference Fig.14 The heating assembly includes a heating plate 29 and an electric heating wire 30. The heating plate 29 is connected to the upper box body 1, and the electric heating wire 30 is connected to the heating plate 29. The electric heating wire 30 is arranged on the lower side of the placement seat 7. The electric heating wire 30 works to heat the cage body 8 on the placement seat 7. A temperature detector 31 is provided in the placement seat 7. The temperature of the electric heating wire 30 when heating the heating medium 44 is detected by the temperature detector 31, so as to prevent the mouse from being damaged by excessive temperature.

[0056] Reference Fig.15 and 17, slide plates 32 are provided at both ends of the heating plate 29, and a guide groove 41 for the slide plate 32 to slide is provided on the upper box body 1. A limit plate 33 is provided at one end of the slide plate 32 extending outside the upper box body 1, and a first grip ring 34 is provided on the limit plate 33. Moving the first grip ring 34 causes the limit plate 33 to move, and then the slide plate 32 connected to the limit plate 33 moves in the guide groove 41, thereby realizing the position adjustment of the heating plate 29 in the upper box body 1, so that the heating component performs heat stress work on mice of different sizes in the cage 8 on the placement seat 7, and the position of the slide plate 32 is limited by the limit plate 33. In this embodiment, the number of heating plates 29 is two, and four guide grooves 41 are provided on both sides of the upper box body 1, and the length of the guide groove 41 is greater than the width of the slide plate 32.

[0057] Reference Figure 4-6 The vibration mechanism includes a motor 16, a first gear 17, a second gear 18, a connecting shaft 19, a third gear 20, a rotating shaft 21, a cam 22 and a moving plate 23. The motor 16 is connected to the lower box body 2, the power output shaft of the motor 16 is connected to the first gear 17, the connecting shaft 19 is connected to the lower box body 2, the connecting shaft 19 is connected to the second gear 18, the first gear 17 is meshed with the second gear 18, the rotating shaft 21 is connected to the lower box body 2, the rotating shaft 21 is connected to the third gear 20, the third gear 20 is meshed with the second gear 18, the rotating shaft 21 is connected to the cam 22, and one end of the moving plate 23 is in contact with the moving seat 8. The other end of the movable plate 23 contacts the cam 22, and the motor 16 works. The power output shaft of the motor 16 rotates to rotate the first gear 17. The rotation of the first gear 17 causes the second gear 18 to rotate under the support of the connecting shaft 19. The second gear 20 rotates to cause the third gear 20 meshing therewith to rotate. The rotation of the third gear 20 causes the rotating shaft 21 to rotate. The rotation of the rotating shaft 21 causes the cam 22 to rotate. The rotation of the cam 22 causes the movable plate 23 in contact with it to move up and down. The movable plate 23 moves up and down to drive the placement seat 7 to move in the cage body 8, thereby achieving vibration of the mice in the experimental box 100.

[0058] Specifically, two rotating shafts 21 and two connecting shafts 19 are provided in the lower box body 2, two second gears 18 are respectively provided on the two connecting shafts 19, and the two second gears 18 are arranged on both sides of the first gear 17. Two third gears 20 are respectively installed on the two rotating shafts 21, and the two third gears 20 are respectively meshed with the two second gears 18. The rotating shafts 21 and the connecting shafts 19 are connected to the inner wall of the lower box body 2 through bearings, and three cams 22 are provided on each rotating shaft 21. Correspondingly, six movable plates 23 are provided on the upper side of the six cams 22, and the six movable plates 23 are used to realize the vibration of the six placement seats 7.

[0059] Reference Figure 4 and 7A first guide cylinder 14 is provided at one end of the placement seat 7 close to the upper cover 3, and a guide column 15 is provided on the upper cover 3. The first guide cylinder 14 is sleeved on the guide column 15, and the movement of the placement seat 8 is guided by the cooperation of the first guide cylinder 14 and the guide column 15. In this embodiment, the first guide cylinder 14 is welded to the placement seat 7, and the guide column 15 is welded to the upper cover 3.

[0060] A second guide cylinder 26 is provided on the side of the moving plate 23 close to the placement seat 7, and a third guide cylinder 27 is provided on the placement seat 7. The second guide cylinder 26 is sleeved on the third guide cylinder 27, and a spring 28 is provided between the second guide cylinder 26 and the third guide cylinder 27. The elasticity of the spring 28 realizes the elastic contact between the moving plate 23 and the placement seat 8, thereby preventing the moving plate 23 and the placement seat 8 from being damaged during the vibration process, and the second guide cylinder 26 and the third guide cylinder 27 guide the movement of the placement seat 8 and the moving plate 23. A contact block 24 is provided at one end of the moving plate 23 close to the cam 22, and an abutment surface 25 contacting the cam 22 is provided on the contact block 24. When the cam 22 rotates, the cam 22 is always in contact with the abutment surface 25 on the contact block 24, so that the contact block 24 can be moved up and down under the extrusion of the cam 22 and the action of gravity, thereby realizing the up and down movement of the entire moving plate 23, the placement seat 7 and the cage body 8.

[0061] A holder 35 is provided in the placement hole 9, and a clamping plate 36 is provided on one side of the holder 35 close to the cage body 8. The holder 36 contacts the cover 10, and an avoidance groove 38 is provided on the holder 36. A second gripping ring 37 is provided on the holder 36. The holder 35 is placed in the placement hole 9. At this time, the clamping plate 36 on the holder 35 supports the cover 10, thereby supporting the cage body 8 and the placement seat 7. Then, the cam 22 rotates so that the extrusion spring 28 cannot vibrate the placement seat 7, so that the experimenter can fix the cage body 8 as needed. The fixing of the cage body 8 is convenient and fast, and it is easy to use. The second gripping ring 37 arranged on the holder 35 facilitates the movement of the holder 35.

[0062] A control panel 39 is provided on the upper cover 3 , and the motor 16 and the electric heating wire 30 are controlled by the control panel 39 .

[0063] When using the above device for experiments, four experimental groups and one control group were set up. The control group (Controll) was a group that did not subject mice to restraint heat stress at room temperature. The remaining four experimental groups were:

[0064] Mice were heat-stressed at 37°C for 1 h;

[0065] Mice were heat-stressed at 37°C for 2 h;

[0066] Mice were heat-stressed at 39°C for 1 h;

[0067] Mice were heat stressed at 39°C for 2 h.

[0068] Specifically, the mouse is placed in the separation cage 45, and then the separation cage 45 is placed in the cage body 8. The user holds the third grip ring 45 to rotate the cover 10 to close the cage body 8, and fixes the cover 10 to the side end of the cage body 8 by the third bolt 11, thereby achieving the closure of the cage body 8 in which the mouse is placed. At this time, the cage body 8 is inserted into the placement seat 7, and water is added to the cage body 8 through the injection port 43 on the cover 10. At this time, the control panel 39 is used to control the electric heating wire 30 on the heating plate 29 in the experimental box 100 to work, so as to heat the water in the cage body 8 in the placement seat 7, and the mouse testicles in the separation cage 42 in the cage body 8 are locally stably heated by the water, and the temperature detector 31 in the placement seat 7 detects the stability of the water, thereby ensuring the control of the water temperature;

[0069] The motor 16 in the experimental box 100 is controlled by the control panel 39. The operation of the motor 16 causes the first gear 17 to rotate. The rotation of the first gear 17 causes the second gear 18 to rotate under the support of the connecting shaft 19. The second gear 20 rotates, causing the third gear 20 meshing therewith to rotate. The rotation of the third gear 20 causes the rotating shaft 21 to rotate. The rotation of the rotating shaft 21 causes the cam 22 to rotate. The rotation of the cam 22 causes the movable plate 23 in contact with it to move up and down. The movable plate 23 moves up and down, driving the placement seat 7 to move in the cage 8, thereby achieving vibration of the mice in the experimental box 100 to prevent the mice from sleeping during the heat stress of summer heat.

[0070] The mice were subjected to continuous restraint heat stress for 2 weeks, and the testicular tissues were tested. Figure 18-26 .

[0071] in, Fig.25 DDX4 in the germ cells is a marker protein. Fig.25 This is the result of staining using immunohistochemistry.

[0072] Fig.26 SYCP3 in the spermatocytes is a marker protein in the first meiotic division. Fig.26 This is the result of staining using immunohistochemistry.

[0073] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions and improvements made within the spirit and principles of the present invention should be included in the protection scope of the present invention.

Claims

1. A restrained heat stress experimental device, characterized in that: The experimental box (100) comprises a placement seat (7) provided in the experimental box (100), a cage (8) provided in the placement seat (7), a heating component provided in the experimental box (100), and a vibration component for driving the cage (8) to vibrate provided in the experimental box (100).

2. The restrained heat stress experimental device according to claim 1, characterized in that: The experimental box (100) comprises an upper box body (1), a lower box body (2) and an upper cover (3); a side plate (5) is provided between the upper box body (1) and the lower box body (2); the side plate (5) is connected to the upper box body (1) and the lower box body (2) via a second bolt (6); the upper cover (3) is connected to the upper box body (1) via a first bolt (4); and the upper box body (1) is provided with a placement hole (9) for a cage body (8) to move.

3. The restrained heat stress experimental device according to claim 2, characterized in that: The cage body (8) is provided with a slide column (12), the placement seat (7) is provided with a slide groove (13) matched with the slide column (12), the cage body (8) is provided with a cover (10), one end of the cover (10) is hinged to the cage body (8), and the other end of the cover (10) is connected to the cage body (8) through a third bolt (11), and an empty groove (40) is provided on the lower side of the placement seat (7).

4. The restrained heat stress experimental device according to claim 3, characterized in that: The cage body 8 is provided with a partition cage 42, the cover 10 is provided with a liquid injection port 43, the placement seat 7 is provided with a heating medium 44, the cover 10 is provided with a third grip ring 45, and the upper box body 1, the prevention seat 7 and the cage body 8 are all provided with ventilation holes.

5. The restrained heat stress experimental device according to claim 3, characterized in that: The heating assembly comprises a heating plate (29) and an electric heating wire (30); the heating plate (29) is connected to the upper box (1); the electric heating wire (30) is connected to the heating plate (29); the electric heating wire (30) is arranged on the lower side of the placement seat (7); and a temperature detector (31) is arranged in the placement seat (7).

6. The restrained heat stress test device according to claim 4, characterized in that: Slide plates (32) are provided at both ends of the heating plate (29), a guide groove (41) for the slide plate (32) to slide is provided on the upper box body (1), a limit plate (33) is provided at one end of the slide plate (32) extending outside the upper box body (1), and a first grip ring (34) is provided on the limit plate (33).

7. The restrained heat stress test device according to any one of claims 4 to 6, characterized in that: The vibration mechanism comprises a motor (16), a first gear (17), a second gear (18), a connecting shaft (19), a third gear (20), a rotating shaft (21), a cam (22) and a moving plate (23); the motor (16) is connected to the lower housing (2); the power output shaft of the motor (16) is connected to the first gear (17); the connecting shaft (19) is connected to the lower housing (2); the connecting shaft (19) is connected to the second gear (18); the first gear (17) is meshed with the second gear (18); the rotating shaft (21) is connected to the lower housing (2); the rotating shaft (21) is connected to the third gear (20); the third gear (20) is meshed with the second gear (18); the rotating shaft (21) is connected to the cam (22); one end of the moving plate (23 is in contact with the moving seat (8); and the other end of the moving plate (23) is in contact with the cam (22).

8. The restrained heat stress test device according to claim 7, characterized in that: A first guide cylinder (14) is provided at one end of the placement seat (7) close to the upper cover (3), a guide column (15) is provided on the upper cover (3), and the first guide cylinder (14) is sleeved on the guide column (15).

9. The restrained heat stress test device according to claim 8, characterized in that: A second guide cylinder (26) is provided on one side of the movable plate (23) close to the placement seat (7); a third guide cylinder (27) is provided on the placement seat (7); the second guide cylinder (26) is sleeved on the third guide cylinder (27); a spring (28) is provided between the second guide cylinder (26) and the third guide cylinder (27); a contact block (24) is provided on one end of the movable plate (23) close to the cam (22); and a contact surface (25) contacting the cam (22) is provided on the contact block (24).

10. The restrained heat stress test device according to claim 9, characterized in that: A card seat (35) is provided in the placement hole (9), a card plate (36) is provided on a side of the card seat (35) close to the cage body (8), the card seat (36) is in contact with the cover (10), an avoidance groove (38) is provided on the card seat (36), a second grip ring (37) is provided on the card seat (36), and a control panel (39) is provided on the upper cover (3).

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