Anti-hair loss natural plant composition and application thereof

Abstract

The invention discloses an anti-hair-loss natural plant composition and application thereof. The anti-hair-loss natural plant composition comprises the following components: a cacumen biotae alcohol extract, a ginseng aqueous extract, a gynostemma pentaphylla alcohol extract and undaria pinnatifida crude polysaccharide. The anti-hair loss and hair growth activity of the composition is evaluated by establishing an androgenetic alopecia mouse model, the result shows that the composition can accelerate the transition of hair follicles of the androgenetic alopecia mouse from a resting period to a growing period, and the hair growth length of the androgenetic alopecia mouse model reaches 9.94 + / -0.13 mm 21 days after the composition is externally applied. The hair coverage rate reaches 96.74 + / -1.98%, the vellus hair proportion is 20.1%, and the composition can significantly improve the hair growth speed of mice with androgenetic alopecia.

Description

Technical Field

[0001] The invention belongs to the field of cosmetics / hair washing and hair care, and in particular relates to a natural plant composition for preventing hair loss and application thereof. Background Art

[0002] Androgenetic alopecia (AGA) is the most common pathological type of hair loss in clinical practice. Although androgenetic alopecia is not a serious disease, it can affect a person's mental outlook, thus causing adverse effects on the patient's daily work and life.

[0003] There are various methods for treating androgenic alopecia, but the pros and cons vary. The surgical hair transplant process is high-risk and expensive, and drug treatments including finasteride and minoxidil have obvious side effects after long-term use and are prone to tolerance. In recent years, the application of natural active substances in the daily chemical industry has become a hot spot for the development of anti-hair loss products. Anti-hair loss products containing natural active substances are increasingly favored by consumers because of their natural safety, mildness, effectiveness, and comprehensive effects.

[0004] CN115645342B discloses a composition with anti-hair loss effect and its application in the preparation of anti-hair loss shampoo, which is prepared from the following raw materials: 3-6 parts of Ligustrum lucidum fruit, 2-5 parts of Platycladus orientalis leaves, 2-5 parts of ginger, 1-3 parts of Polygonum multiflorum, 1-3 parts of Salvia miltiorrhiza, and 4-5 parts of auxiliary additives. However, the extraction method of the invented composition is to extract water after mixing all the raw materials according to the formula ratio. The traditional water extraction method can only extract the water-soluble components in the raw materials such as polysaccharides, flavonoids or phenolic substances, but cannot extract the water-insoluble active components therein.

[0005] CN118649109A discloses an anti-hair loss composition extraction method, an anti-hair loss composition and uses, comprising the following raw materials: Platycladus orientalis leaves, Ganoderma lucidum and ginseng roots, etc. The composition is extracted by sequentially mixing multiple raw materials for water extraction and alcohol precipitation. The extraction process is complicated and the cycle is long. The extract is only macromolecular components such as polysaccharides and proteins, and the anti-hair loss effect of the composition is not obvious.

[0006] Although there are many kinds of anti-hair loss compositions on the market, most of them are only slightly effective at the beginning and not effective after long-term use. In addition, some hair growth products contain irritating ingredients that can cause allergic symptoms such as increased dandruff and itchy skin, bringing additional burden to hair loss patients.

[0007] Although the compositions disclosed in the prior art can relieve the pressure caused by hair loss to a certain extent, these compositions cannot achieve the ideal anti-hair loss effect. It is urgent to develop an anti-hair loss and hair growth composition with better effect, mild to the scalp and rich in natural active ingredients to fundamentally solve the trouble of hair loss. Summary of the invention

[0008] In view of the shortcomings of the prior art, the present invention provides a natural plant composition for preventing hair loss and its application. Based on the previous review of a large number of relevant product literature, the present invention screened up to 24 plant extracts (water extracts and ethanol extracts of ginger, Polygonum multiflorum, ginseng, Platycladus orientalis leaves, Astragalus, Scutellaria baicalensis, Polygonatum sibiricum, Camellia sinensis, Pinus massoniana needles, Panax notoginseng, Cuscuta australis, Angelica sinensis, Tangerine peel, Chrysanthemum, Ligustrum lucidum, Rehmannia glutinosa, Dendrobium officinale, Ganoderma lucidum, Armillaria cirrhosa, Tangerine peel, Gentiana scabra, Cortex Moutan, Eclipta prostrata, Usciola ursi and the like) for their anti-hair loss activity using an androgenic alopecia mouse experimental model (for relevant results, see Figure 5 , Figure 6 , Figure 7 , Table 5, Table 6, Table 7), the results show that Platycladus orientalis leaf 70% ethanol extract, ginseng water extract, Gynostemma pentaphyllum 70% ethanol extract and Undaria pinnatifida crude polysaccharide have better anti-hair loss effect than other natural plant extracts. Orthogonal test shows that the combination of Platycladus orientalis leaf 70% ethanol extract, ginseng water extract, Gynostemma pentaphyllum 70% ethanol extract and Undaria pinnatifida crude polysaccharide can accelerate the transition of hair follicles from the resting phase to the growth phase in mice with androgenic alopecia, and significantly increase the hair growth rate of mice with androgenic alopecia. The anti-hair loss effect of this combination is significantly better than the existing technology and similar products, and a safe and effective natural extract product that nourishes the scalp and has anti-hair loss effect can be developed.

[0009] The anti-hair loss natural plant composition of the present invention comprises the following components:

[0010] The mass ratio of Platycladus orientalis leaf alcohol extract, ginseng water extract, Gynostemma pentaphyllum alcohol extract and Undaria pinnatifida crude polysaccharide is 10:9:7:6.

[0011] The ethanol extract of Platycladus orientalis leaves is obtained by extraction by the following method:

[0012] The Platycladus orientalis leaves were cut into small pieces, impurities were removed, and the powder was crushed into fine powder by a grinder, and passed through a 20-mesh sieve; the Platycladus orientalis leaf powder was mixed with 70vt% ethanol solution at a solid-liquid ratio of 1g:10mL, and refluxed in a water bath for 1h, the extraction was repeated 3 times, and the extract was collected by centrifugation; the extract was concentrated and freeze-dried to obtain the Platycladus orientalis leaf alcohol extract.

[0013] The ginseng water extract is obtained by extraction by the following method:

[0014] Cut ginseng into small pieces, remove impurities, grind into fine powder, and pass through a 20-mesh sieve; mix ginseng powder with pure water at a solid-liquid ratio of 1 g:10 mL, extract in a 90°C water bath for 1 h, repeat the extraction 3 times, and collect the extract by centrifugation; concentrate the extract and freeze-dry it to obtain a ginseng water extract.

[0015] The Gynostemma pentaphyllum ethanol extract is obtained by extraction by the following method:

[0016] Cut Gynostemma pentaphyllum into small pieces, remove impurities, grind into fine powder, and pass through a 20-mesh sieve; mix the Gynostemma pentaphyllum powder with 70vt% ethanol solution according to a solid-liquid ratio of 1g:20mL, reflux extract in a water bath at 80°C for 1h, repeat the extraction 3 times, and collect the extract by centrifugation; concentrate the extract and freeze-dry it to obtain the Gynostemma pentaphyllum alcohol extract.

[0017] The crude polysaccharide of Undaria pinnatifida is extracted by the following method:

[0018] Cut the kelp into small pieces, remove impurities, grind into fine powder, and pass through a 20-mesh sieve; mix the kelp powder with pure water at a solid-liquid ratio of 1g:30mL, extract in a water bath at 90°C for 1h, repeat the extraction 3 times, collect the supernatant by centrifugation and concentrate it to 1 / 4-1 / 5 of the original volume, add 4 times the volume of anhydrous ethanol and mix well, let it stand for 8h (4-10°C), take out the precipitate by centrifugation and add water until it is completely dissolved; then concentrate it to a certain volume (about 1 / 5) and freeze-dry it to obtain crude kelp polysaccharide.

[0019] The invention discloses an application of the natural plant composition in preparing a product with the function of preventing hair loss and promoting hair growth.

[0020] The beneficial effects of the present invention are embodied in:

[0021] 1. The anti-hair loss composition of the present invention significantly improves the interaction between the components by adjusting the types and addition amounts of each component. The composition of 70% ethanol extract of Platycladus orientalis leaves, water extract of ginseng, 70% ethanol extract of Gynostemma pentaphyllum and crude polysaccharide of Undaria pinnatifida can nourish hair follicles, which can prevent hair loss and promote hair growth.

[0022] 2. The preparation process of the anti-hair loss composition of the present invention is simple, and plant extracts are used as raw materials, such as 70% ethanol extract of Platycladus orientalis leaves, ginseng water extract, 70% ethanol extract of Gynostemma pentaphyllum and crude polysaccharide of Undaria pinnatifida, etc. These ingredients are natural, green and low-toxic, mild to the skin, and will not cause irritation and damage to the scalp and hair, and are suitable for long-term use. In addition, these natural plant extracts have significant antioxidant, anti-inflammatory and antibacterial activities, which can reduce the production of dandruff and oil, and effectively improve the scalp environment. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 This is a diagram showing the hair growth effect of the mouse experiment of the present invention.

[0024] Figure 2 This is the H&E stained section of mouse skin of the present invention.

[0025] Figure 3 The results of the statistical analysis of the proportion of vellus hair in mouse skin sections. Note: The values ​​are expressed as mean ± standard deviation (n = 4). The values ​​with different letters indicate significant differences (P < 0.05 or P < 0.01). Compared with the model group, #P < 0.05 and ## P<0.01.

[0026] Figure 4 Calculate results for orthogonal experimental designs.

[0027] Figure 5 This is a diagram of the hair growth effect on mice when screening plant extracts.

[0028] Figure 6 H&E stained sections of mouse skin for screening of plant extracts.

[0029] Figure 7 The results of the statistical analysis of the proportion of vellus hair in mouse skin sections. Note: The values ​​are expressed as mean ± standard deviation (n = 4). The values ​​with different letters indicate significant differences (P < 0.05 or P < 0.01). Compared with the model group, # P < 0.05 and ## P<0.01. DETAILED DESCRIPTION

[0030] The present invention is described in detail below by way of examples. It must be pointed out that the following examples are used to illustrate the present invention and should not be construed as limiting the present invention.

[0031] Embodiment 1:

[0032] The natural plant composition for preventing hair loss in this embodiment contains 70% ethanol extract of Platycladus orientalis leaves, water extract of Panax ginseng, 70% ethanol extract of Gynostemma pentaphyllum and crude polysaccharide of Undaria pinnatifida.

[0033] The preparation steps include:

[0034] 1) Raw material pretreatment: Cut Platycladus orientalis leaves, ginseng, Gynostemma pentaphyllum, and Undaria pinnatifida into smaller pieces, remove impurities, and select dry materials.

[0035] 2) Powder preparation: Grind the pretreated raw materials into fine powder and pass through a 20-mesh sieve.

[0036] 3) Extract preparation: Extract the Platycladus orientalis leaves, ginseng, Gynostemma pentaphyllum and Undaria pinnatifida respectively.

[0037] The extraction steps of Platycladus orientalis leaves were as follows: mixed with the prepared 70% ethanol solution at a solid-liquid ratio of 1:10 g / ml, refluxed in a water bath at 80° C. for 1 hour, repeated extraction 3 times, and the extract was collected by centrifugation.

[0038] Extraction steps of ginseng: mix with pure water at a solid-liquid ratio of 1:10g / ml, water bath at 90℃ for 1h, repeat the extraction 3 times, and collect the extract by centrifugation.

[0039] The extraction steps of Gynostemma pentaphyllum are as follows: mixing with the prepared 70% ethanol solution at a solid-liquid ratio of 1:20 g / ml, reflux in a water bath at 80° C. for 1 hour, repeating the extraction 3 times, and collecting the extract after centrifugation.

[0040] Extraction steps of kelp: mix with pure water at a solid-liquid ratio of 1:30g / ml, water bath at 90℃ for 1h, repeat the extraction 3 times, collect the supernatant after centrifugation and concentrate it by rotary evaporation to 1 / 4~1 / 5 of the original volume, add 4 times the volume of anhydrous ethanol, mix well and let stand for 8h (4~10℃), centrifuge to obtain the precipitate and add water until it is completely dissolved.

[0041] Concentration and drying: The above four extracts were concentrated to 1 / 4 to 1 / 5 of the original volume and then freeze-dried to obtain 70% ethanol extract of Platycladus orientalis leaves, water extract of ginseng, 70% ethanol extract of Gynostemma pentaphyllum and crude polysaccharide of Undaria pinnatifida.

[0042] Example 2: Anti-hair loss effect test

[0043] The effects of the composition of the Platycladus orientalis leaf 70% ethanol extract, ginseng water extract, Gynostemma pentaphyllum 70% ethanol extract and Undaria pinnatifida crude polysaccharide on hair growth in androgenic alopecia mice were tested.

[0044] Experimental subjects: C57 / BL6 mice, male, weighing between 20-25 g, were randomly divided into 32 groups, 4 mice in each group.

[0045] Androgen mouse model: Each mouse was injected with testosterone propionate for 5 weeks at a dose of 5 mg / kg·d.

[0046] The following 30 solutions were prepared as test solutions, the ratios of the 25 composite compatibility groups are shown in Table 1, and the concentrations of each level are shown in Table 2. In addition, arborvitae leaf ethanol extract solution (100 mg / ml), ginseng water extract solution (100 mg / ml), Gynostemma pentaphyllum ethanol extract solution (100 mg / ml), and Undaria pinnatifida crude polysaccharide solution (100 mg / ml) were prepared as comparisons of the composite compatibility groups, the blank group was prepared with normal saline, and the positive group was prepared with 5% minoxidil solution.

[0047] Table 1. Orthogonal experimental design table

[0048]

[0049]

[0050] Note: A: 70% ethanol extract of Platycladus orientalis leaves; B: water extract of Panax ginseng; C: 70% ethanol extract of Gynostemma pentaphyllum; D: crude polysaccharide of Undaria pinnatifida; 1, 2, 3, 4, 5 are the concentration levels of each extract.

[0051] Table 2. Level comparison table

[0052]

[0053] Experimental method: Each group of mice was given the drug once a day, and 100 μL was evenly applied to the hair loss area, and the drug was continuously used until the skin entered the resting phase. The transition time from the resting phase to the growth phase of each mouse was recorded, and the hair growth in the back area of ​​the mouse was recorded every 3 days. 40 new hairs in the hair loss area on the back of each group of mice were randomly plucked, and the average hair length was measured with a vernier caliper. The changes in hair coverage and average hair length of each test mouse over time were statistically analyzed. After 21 days, the mice were killed by cervical dislocation after blood was drawn from the eye sockets. The skin of the hair loss area on the back was taken for H&E staining, the morphology of the hair follicles on the back skin of the mice was observed, and the proportion of vellus hair was counted.

[0054] Table 3. Mouse hair coverage

[0055]

[0056]

[0057] It can be seen from the data in Table 3 that among the compositions containing arborvitae leaf ethanol extract, ginseng water extract, gynostemma pentaphyllum ethanol extract and kelp polysaccharide, the compositions in groups 14, 19 and 25 can significantly increase the number of hairs per unit area in mice with androgenic alopecia. The hair length of mice was measured using Image J software. The statistical data of hair coverage are shown in Table 3. Among the extract groups, on the 12th day of application, the melanin deposition on the back skin of mice in groups 14 (A3B4C5D2), 19 (A4B4C3D1) and 25 (A5B5C2D1) was deeper, and the hair coverage rate was always higher during the change of time, indicating that the hair growth rate of these three groups of extracts was faster. Among them, the hair coverage rate of group 19 (A4B4C3D1) was close to that of the positive group.

[0058] Table 4. Average hair length of mice

[0059]

[0060]

[0061] It can be seen from the data in Table 4 that among the compositions containing Platycladus orientalis leaf extract, ginseng water extract, Gynostemma pentaphyllum alcohol extract and Undaria pinnatifida polysaccharide, the 14th group (A3B4C5D2), the 19th group (A4B4C3D1) and the 25th group (A5B5C2D1) were significantly superior to other control groups in shortening the hair resting phase and prolonging the growth phase of androgenic alopecia mice. The hair growth length of mice was measured using Image J software. The statistical hair growth length data are shown in Table 4. Among the extract groups, on the 12th day of application, the melanin deposition on the back skin of mice in the 14th group (A3B4C5D2), the 19th group (A4B4C3D1) and the 25th group (A5B5C2D1) groups was deeper, and the average hair length was longer, indicating that the hair growth rate of these three groups of extracts was faster. Among them, the hair growth rate of the 19th group (A4B4C3D1) was close to that of the positive group.

[0062] Table 5. Grouping and extraction conditions of 24 plant extracts

[0063]

[0064]

[0065] Table 6. Mouse hair coverage

[0066]

[0067]

[0068] Table 7. Average hair length of mice

[0069]

[0070]

[0071] Figure 1 The back skin condition of each group of mice in the orthogonal experiment from the 9th to the 21st day of drug administration. The mice were depilated on the back in the sixth week of modeling. The back skin of the mice was smooth and light pink. In the next three weeks, 100 μl of the prepared medicine of each group was continuously applied to the back of the mice every day. At the same time, photos were taken every 3 days to record the back skin condition of the mice. On the 6th day, the back skin of the mice in group 19 (A4B4C3D1) turned slightly black, indicating that the back hair entered the growth phase from the resting phase. There was no obvious change in the back skin of the mice in the other groups. On the 9th day, the back skin of the mice in each group turned black, and hair grew on the back. On the 12th day, the hair of the mice in each group had grown, but the hair coverage area was different and the hair growth rate was different.

[0072] The morphology of mouse hair follicles is an important observation indicator. The morphology of C57 mouse hair follicles in natural state is shown in the following figure. Figure 2As shown in the blank group, more than 80% of the hair follicles are in the natural growth phase, with a round, full appearance and regular shape (terminal hair); while a small number of hair follicles are in the natural resting phase, shrinking, degenerating or even disappearing (vellus hair). In addition, the sebaceous glands scattered on the back skin of mice are accessory glands that secrete oil. Whether there is obvious oil secretion on the back of mice is one of the important criteria for judging whether the model is successful. By observing and counting the morphology and number of hair follicles in the sections, compared with the blank group mice, the sebaceous glands of the hair follicles in the model group mice became larger, the oil secretion was vigorous, and the hair follicles in a large area were reduced, that is, the proportion of vellus hair increased, verifying the success of the model.

[0073] Statistical analysis of the vellus hair ratio in mouse skin sections Figure 3 As shown. The vellus hair ratio of the model group reached 57.28%, which is 2.58 times that of the blank group and 1.85 times that of the positive group. Compared with the model group, among the compositions containing 70% ethanol extract of Platycladus orientalis leaves, ginseng water extract, 70% ethanol extract of Gynostemma pentaphyllum and Undaria pinnatifida polysaccharide, the vellus hair ratio of Group 19 (A4B4C3D1) was the lowest, at 20.9%, followed by Group 25 (A5B5C2D1), with a vellus hair ratio of 23.68%, and Group 14 (A3B4C5D2), with a vellus hair ratio of 25.11%. The results showed that with the increase in the proportion of Platycladus orientalis and ginseng extracts, the vellus hair ratio gradually decreased, and the anti-hair loss effect showed an upward trend.

[0074] The results of the orthogonal experiment showed that when the ratio of the composition containing 70% ethanol extract of Platycladus orientalis leaves, ginseng water extract, 70% ethanol extract of Gynostemma pentaphyllum and kelp polysaccharide was 10:9:7:6, the hair growth rate of mice was the fastest and the anti-hair loss effect was the best. The experiment on mice with androgenic alopecia showed that when the ratio of the composition was 10:9:7:6, the average hair length of mice on the 21st day reached 9.94±0.13mm, the hair coverage rate reached 96.74±1.98%, the proportion of vellus hair was 20.1%, and the hair growth rate of mice was faster than that of other groups. Compared with the model group, the anti-hair loss effect was extremely significant.

[0075] The above embodiments are preferred implementation modes of the present invention, but the implementation modes of the present invention are not limited to the above embodiments. Any other changes, modifications, substitutions, combinations, and simplifications that do not deviate from the spirit and principles of the present invention should be equivalent replacement methods and are included in the protection scope of the present invention.

Claims

1. A natural plant composition for preventing hair loss, characterized in that Includes the following components: Alcohol extract of Platycladus orientalis leaves, water extract of Panax ginseng, alcohol extract of Gynostemma pentaphyllum and crude polysaccharide of Undaria pinnatifida.

2. The anti-hair loss natural plant composition according to claim 1, characterized in that The ethanol extract of Platycladus orientalis leaves is obtained by extraction by the following method: Cut the Platycladus orientalis leaves into small pieces, remove impurities, grind into fine powder, and pass through a 20-mesh sieve; mix the Platycladus orientalis leaf powder with ethanol solution, reflux extract in a water bath at 80°C for 1 hour, repeat the extraction 3 times, and collect the extract after centrifugation; The obtained extract was concentrated and then freeze-dried to obtain the ethanol extract of Platycladus orientalis leaves.

3. The anti-hair loss natural plant composition according to claim 2, characterized in that: The volume concentration of the ethanol solution is 70 vt%.

4. The anti-hair loss natural plant composition according to claim 1, characterized in that The ginseng water extract is obtained by extraction by the following method: Cut ginseng into small pieces, remove impurities, grind into fine powder, and pass through a 20-mesh sieve; mix the ginseng powder with double distilled water, extract in a water bath at 90°C for 1 hour, repeat the extraction 3 times, collect the extract after centrifugation; concentrate the obtained extract and freeze-dry it to obtain ginseng water extract.

5. The anti-hair loss natural plant composition according to claim 1, characterized in that The Gynostemma pentaphyllum ethanol extract is obtained by extraction by the following method: Cut Gynostemma pentaphyllum into small pieces, remove impurities, grind into fine powder, and pass through a 20-mesh sieve; mix the Gynostemma pentaphyllum powder with ethanol solution, extract under reflux in a water bath for 1 hour, repeat the extraction 3 times, and collect the extract after centrifugation; The obtained extract was concentrated and then freeze-dried to obtain the Gynostemma pentaphyllum ethanol extract.

6. The anti-hair loss natural plant composition according to claim 5, characterized in that: The volume concentration of the ethanol solution is 70 vt%.

7. The anti-hair loss natural plant composition according to claim 1, characterized in that The crude polysaccharide of Undaria pinnatifida is extracted by the following method: Cut the kelp into small pieces, remove impurities, grind into fine powder, and pass through a 20-mesh sieve; mix the kelp powder with double distilled water, extract in a water bath at 70°C for 1 hour, repeat the extraction 3 times, collect the supernatant after centrifugation and concentrate it by rotary evaporation to 1 / 4 to 1 / 5 of the original volume, add four times the volume of anhydrous ethanol and mix well, then stand at 4 to 10°C for 8 hours, take the precipitate after centrifugation and add water until it is completely dissolved; then concentrate it to 1 / 5 of the original volume and freeze-dry it to obtain crude kelp polysaccharide.

8. The natural plant composition for preventing hair loss according to any one of claims 1 to 7, characterized in that: The mass ratio of Platycladus orientalis leaf ethanol extract, ginseng water extract, Gynostemma pentaphyllum ethanol extract and Undaria pinnatifida crude polysaccharide is 10:9:7:

6.

9. Use of the anti-hair loss natural plant composition according to any one of claims 1 to 8 in the preparation of a product with anti-hair loss and hair growth function.

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