Whitening and spot-fading composition containing 4-butylresorcinol and application of whitening and spot-fading composition

Abstract

The invention relates to a whitening and spot-fading composition containing 4-butylresorcinol and application thereof, and belongs to the technical field of cosmetics, the whitening and spot-fading composition comprises the following raw materials: black mulberry, spearmint, crithmum maritimum, 4-butylresorcinol, kojic acid, ascorbyl tetraisopalmitate, ethylhexylglycerin and a solvent; the whitening and spot-fading composition further comprises lactobacillus plantarum, lactobacillus rhamnosus and lactobacillus bulgaricus. The black mulberry, the spearmint and the crithmum maritimum are selected for compounding and water extraction, and the lactobacillus plantarum, the lactobacillus rhamnosus and the lactobacillus bulgaricus are used as mixed bacteria to ferment the water extract, so that the prepared composition finished product has excellent oxidation resistance; 4-butylresorcinol and kojic acid enable the prepared composition to have whitening and spot-fading effects, meanwhile, ascorbyl tetraisopalmitate is added to further enhance the whitening and spot-fading effects of the system, and ethylhexylglycerin is added to improve the moisturizing effect of the composition finished product.

Description

Technical Field

[0001] The invention belongs to the technical field of cosmetics, and in particular relates to a whitening and spot-lightening composition containing 4-butylresorcinol and an application thereof. Background Art

[0002] With the growing demand for beauty and skin care, anti-oxidation and whitening and spot-lightening have become the functional needs that attract much attention in the skin care market. Anti-oxidation refers to preventing oxidation reactions caused by free radicals, thereby protecting cells from oxidative damage and maintaining skin health and youthfulness, while whitening refers to improving skin pigmentation problems, making the skin bright and fair, and mainly solving skin problems such as freckles, sun spots, and spots. Antioxidant ingredients such as vitamin C, vitamin E, and polyphenols can effectively protect skin cells from external environmental damage (such as ultraviolet rays, pollution, etc.) by neutralizing free radicals and reducing the occurrence of oxidation reactions; whitening ingredients mainly inhibit the enzyme activity of melanin production, reduce the synthesis of melanin, and promote the decomposition and metabolism of melanin, thereby improving skin pigmentation problems.

[0003] Studies have shown that 4-butylresorcinol can inhibit the generation and transfer of melanin to a certain extent, thereby achieving the effect of whitening and lightening spots. However, the use of 4-butylresorcinol alone may have the problem of low utilization and poor effect. Therefore, the development of a composition containing 4-butylresorcinol with antioxidant and whitening and lightening effects can not only give full play to the advantages of 4-butylresorcinol, but also overcome its shortcomings when used alone, which has important practical significance and broad market prospects. Summary of the invention

[0004] The purpose of the present invention is to provide a whitening and freckle-removing composition containing 4-butylresorcinol and its application, which solves the problem that the existing composition has poor antioxidant and whitening and freckle-removing effects.

[0005] The purpose of the present invention can be achieved through the following technical solutions:

[0006] A whitening and spot-lightening composition containing 4-butylresorcinol, the whitening and spot-lightening composition specifically comprises the following raw materials in parts by weight:

[0007]

[0008] Black mulberry is the ripe fruit of the black mulberry tree of the Moraceae family. It is rich in vitamins, polyphenols, amino acids, trace elements and other ingredients. Its polyphenol compounds, especially anthocyanins, have the highest content, so it has the effect of scavenging free radicals.

[0009] Spearmint is a herbaceous plant of the Lamiaceae family. It is rich in carvone, phenolic acid compounds and flavonoid compounds, and has good total antioxidant capacity.

[0010] Sea fennel is a perennial plant of the Umbelliferae family. It contains chlorogenic acid, polyphenols, vitamin C, flavonoids, organic acids and other beneficial ingredients with strong antioxidant activity.

[0011] 4-Butylresorcinol can inhibit the production and transfer of melanin to a certain extent;

[0012] Kojic acid can combine with the copper ions in tyrosinase, making it inactive, effectively helping to lighten spots and make the skin fairer;

[0013] Ascorbyl tetraisopalmitate is esterified with palmitic acid and natural ingredients such as L-ascorbic acid. It has all the physiological activities of vitamin C and overcomes the three major shortcomings of vitamin C: fear of heat, light and moisture. It has excellent stability and can be quickly absorbed by the skin and converted into active vitamin C in cells, thus showing a certain whitening effect and helping to relieve dry skin.

[0014] Ethylhexylglycerin has a good moisturizing effect. It can not only help lock the moisture on the skin surface, but also reduce the loss of internal moisture, making the skin more hydrated and getting rid of the problem of dry skin.

[0015] Furthermore, the solvent includes deionized water and butanediol.

[0016] Furthermore, the deionized water and butanediol are composed in a mass ratio of 4-5:0.3-0.4.

[0017] As a preferred technical solution of the present invention, the whitening and spot-lightening composition further comprises Lactobacillus plantarum, Lactobacillus rhamnosus and Lactobacillus bulgaricus.

[0018] Furthermore, the viable count of the plant lactobacillus is 1.8×10 7 CFU / mL, the viable count of Lactobacillus rhamnosus is 1×10 9 CFU / mL, the viable count of Lactobacillus bulgaricus was 1.7×10 7 CFU / mL.

[0019] As a preferred technical solution of the present invention, the preparation method of the whitening and spot-lightening composition containing 4-butylresorcinol comprises the following steps:

[0020] S10, activating the strains and pretreating part of the raw materials to obtain activated Lactobacillus plantarum, activated Lactobacillus rhamnosus, activated Lactobacillus bulgaricus, sterilized water extract, mixed solution A and mixed solution B;

[0021] S20, preparing fermentation liquid: uniformly mixing the activated Lactobacillus plantarum, the activated Lactobacillus rhamnosus and the activated Lactobacillus bulgaricus to obtain a mixed bacterial agent, adding the mixed bacterial agent to the sterilized water extract, shaking, sealing and fermenting under temperature control to obtain a fermentation material, inactivating under temperature control, filtering and centrifuging to obtain a fermentation liquid;

[0022] S30, kojic acid and deionized water are mixed evenly, and mixed solution B and mixed solution A are added in sequence, and the mixture is stirred evenly under controlled temperature to obtain a finished composition.

[0023] Furthermore, step S10 specifically includes:

[0024] S11, 4-butylresorcinol and butanediol are mixed evenly to obtain a mixed solution A, which is set aside; ascorbyl tetraisopalmitate and ethylhexylglycerol are mixed evenly to obtain a mixed solution B, which is set aside;

[0025] S12, taking 50 μL of Lactobacillus plantarum, Lactobacillus rhamnosus and Lactobacillus bulgaricus respectively, inoculating them into MRS culture medium respectively, and culturing them at a constant temperature to obtain activated Lactobacillus plantarum, activated Lactobacillus rhamnosus and activated Lactobacillus bulgaricus;

[0026] S13. Evenly mix black mulberry, spearmint and sea fennel to obtain a mixture A, evenly mix the mixture A and deionized water, boil the mixture, and filter to obtain a filtrate A and a filter residue A, evenly mix the filter residue A and deionized water, boil the mixture, and obtain a filtrate B and a filter residue B, combine the filtrate A and the filtrate B, concentrate the mixture, and obtain a concentrated solution. The mixture is sterilized by temperature control to obtain a sterilized water extract.

[0027] Furthermore, the temperature of the temperature-controlled constant temperature culture in step S12 is 37° C. and the time is 24 hours.

[0028] Furthermore, in step S13, the volume ratio of the mixture A to deionized water is 1:8-10; the volume ratio of the filter residue A to deionized water is 1:8-10.

[0029] Furthermore, the boiling time in step S13 is 30-40 minutes; the concentration is concentrated to half of the total volume of filtrate A and filtrate B; the temperature of the temperature-controlled sterilization is 121° C., and the time is 30-35 minutes.

[0030] Furthermore, in step S20, the volume ratio of the activated Lactobacillus plantarum, the activated Lactobacillus rhamnosus and the activated Lactobacillus bulgaricus is 3-3.5:1.5-2:1-1.5; and the mixed bacterial agent accounts for 0.3-0.5% of the volume percentage of the sterile water extract.

[0031] Furthermore, in step S20, the temperature of the temperature-controlled sealed fermentation is 28-30°C, and the time is 72 hours; the temperature of the temperature-controlled inactivation is 121°C, and the time is 20-25 minutes.

[0032] Furthermore, the temperature for the temperature-controlled stirring in step S3 is 28-30°C.

[0033] The invention discloses an application of a whitening and lightening spot composition containing 4-butylresorcinol. The whitening and lightening spot composition is added into skin care products at a mass percentage of 3-4%.

[0034] Furthermore, the skin care products include but are not limited to toners, essences, creams, masks and lotions.

[0035] Beneficial effects of the present invention:

[0036] (1) The present invention selects three raw materials, namely, black mulberry, spearmint and sea fennel, for compounding and water extraction, and uses Lactobacillus plantarum, Lactobacillus rhamnosus and Lactobacillus bulgaricus as mixed bacterial agents to ferment the water extract. After the fermentation process is optimized, the superoxide dismutase activity and total phenol content of the fermented liquid obtained are increased, thereby effectively making the prepared finished composition have excellent antioxidant properties.

[0037] (2) The present invention adds 4-butylresorcinol and kojic acid to inhibit melanin and lighten spots, so that the prepared composition has excellent whitening and lightening effects. At the same time, ascorbyl tetraisopalmitate, which has both whitening and certain moisturizing effects, is added to further enhance the whitening and lightening effects of the system and cooperates with ethylhexylglycerin to jointly improve the moisturizing effect of the finished composition; therefore, the finished composition prepared thereby has outstanding whitening and lightening and moisturizing effects. DETAILED DESCRIPTION

[0038] The following will be combined with the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0039] The viable count of Lactobacillus plantarum used in the present invention is 1.8×10 7 CFU / mL, the number of viable Lactobacillus rhamnosus used was 1×10 9 CFU / mL, the viable count of Lactobacillus bulgaricus used was 1.7×10 7 CFU / mL; the above, no further details will be given later.

[0040] Example 1

[0041] A whitening and spot-lightening composition containing 4-butylresorcinol, the whitening and spot-lightening composition specifically comprises the following raw materials in parts by weight:

[0042]

[0043] The solvent comprises deionized water and butanediol in a mass ratio of 4:0.3;

[0044] The whitening and spot-lightening composition also includes Lactobacillus plantarum, Lactobacillus rhamnosus and Lactobacillus bulgaricus;

[0045] The preparation method of the whitening and spot-lightening composition containing 4-butylresorcinol comprises the following steps:

[0046] S10, activating the strains and pre-treating some raw materials:

[0047] S11, 4-butylresorcinol and butanediol are mixed evenly to obtain a mixed solution A, which is set aside; ascorbyl tetraisopalmitate and ethylhexylglycerol are mixed evenly to obtain a mixed solution B, which is set aside;

[0048] S12, taking 50 μL of Lactobacillus plantarum, Lactobacillus rhamnosus and Lactobacillus bulgaricus respectively, inoculating them into MRS culture medium respectively, and culturing them at 37° C. for 24 h to obtain activated Lactobacillus plantarum, activated Lactobacillus rhamnosus and activated Lactobacillus bulgaricus;

[0049] S13, mixing black mulberry, spearmint and sea fennel uniformly to obtain a mixture A, mixing the mixture A and deionized water in a volume ratio of 1:8 uniformly and boiling, filtering to obtain a filtrate A and a filter residue A, mixing the filter residue A and deionized water in a volume ratio of 1:10 uniformly and boiling again to obtain a filtrate B and a filter residue B, combining the filtrate A and the filtrate B and concentrating to half the total volume of the filtrate A and the filtrate B to obtain a concentrated solution, and sterilizing at 121° C. for 30 min to obtain a sterilized water extract;

[0050] S20, preparing fermentation liquid: the activated Lactobacillus plantarum, the activated Lactobacillus rhamnosus and the activated Lactobacillus bulgaricus are mixed uniformly in a volume ratio of 3:2:1 to obtain a mixed bacterial agent, the mixed bacterial agent is added to the sterilized water extract, shaken, sealed and fermented at 28°C for 72h to obtain a fermentation material, inactivated at 121°C for 22.5min, filtered and centrifuged to obtain a fermentation liquid;

[0051] The mixed bacterial agent accounts for 0.4% of the volume percentage of the sterile water extract;

[0052] S30, kojic acid and deionized water are mixed evenly, and mixed solution B and mixed solution A are added in sequence, and the temperature is controlled at 28° C. and stirred evenly to obtain a finished composition.

[0053] Example 2

[0054] A whitening and spot-lightening composition containing 4-butylresorcinol, the whitening and spot-lightening composition specifically comprises the following raw materials in parts by weight:

[0055]

[0056]

[0057] The solvent comprises deionized water and butanediol in a mass ratio of 5:0.35;

[0058] The whitening and spot-lightening composition also includes Lactobacillus plantarum, Lactobacillus rhamnosus and Lactobacillus bulgaricus;

[0059] The preparation method of the whitening and spot-lightening composition containing 4-butylresorcinol comprises the following steps:

[0060] S10, activating the strains and pre-treating some raw materials:

[0061] S11, 4-butylresorcinol and butanediol are mixed evenly to obtain a mixed solution A, which is set aside; ascorbyl tetraisopalmitate and ethylhexylglycerol are mixed evenly to obtain a mixed solution B, which is set aside;

[0062] S12, taking 50 μL of Lactobacillus plantarum, Lactobacillus rhamnosus and Lactobacillus bulgaricus respectively, inoculating them into MRS culture medium respectively, and culturing them at 37° C. for 24 h to obtain activated Lactobacillus plantarum, activated Lactobacillus rhamnosus and activated Lactobacillus bulgaricus;

[0063] S13, mixing black mulberry, spearmint and sea fennel uniformly to obtain a mixture A, mixing the mixture A and deionized water in a volume ratio of 1:9 uniformly and boiling, filtering to obtain a filtrate A and a filter residue A, mixing the filter residue A and deionized water in a volume ratio of 1:8 uniformly and boiling again to obtain a filtrate B and a filter residue B, combining the filtrate A and the filtrate B and concentrating to half the total volume of the filtrate A and the filtrate B to obtain a concentrated solution, and sterilizing at 121° C. for 32.5 min to obtain a sterilized water extract;

[0064] S20, preparing fermentation liquid: mixing the activated Lactobacillus plantarum, the activated Lactobacillus rhamnosus and the activated Lactobacillus bulgaricus in a volume ratio of 3.5:1.75:1.251. uniformly to obtain a mixed bacterial agent, adding the mixed bacterial agent to the sterilized water extract, shaking well, controlling the temperature at 30 ° C, sealing and fermenting for 72 hours to obtain a fermentation material, controlling the temperature at 121 ° C, inactivating for 20 minutes, filtering, centrifuging, and obtaining a fermentation liquid;

[0065] The mixed bacterial agent accounts for 0.5% of the volume percentage of the sterilized water extract;

[0066] S30, kojic acid and deionized water are mixed evenly, and mixed solution B and mixed solution A are added in sequence, and the temperature is controlled at 29° C. and stirred evenly to obtain a finished composition.

[0067] Example 3

[0068] A whitening and spot-lightening composition containing 4-butylresorcinol, the whitening and spot-lightening composition specifically comprises the following raw materials in parts by weight:

[0069]

[0070] The solvent comprises deionized water and butanediol in a mass ratio of 4.5:0.4;

[0071] The whitening and spot-lightening composition also includes Lactobacillus plantarum, Lactobacillus rhamnosus and Lactobacillus bulgaricus;

[0072] The preparation method of the whitening and spot-lightening composition containing 4-butylresorcinol comprises the following steps:

[0073] S10, activating the strains and pre-treating some raw materials:

[0074] S11, 4-butylresorcinol and butanediol are mixed evenly to obtain a mixed solution A, which is set aside; ascorbyl tetraisopalmitate and ethylhexylglycerol are mixed evenly to obtain a mixed solution B, which is set aside;

[0075] S12, taking 50 μL of Lactobacillus plantarum, Lactobacillus rhamnosus and Lactobacillus bulgaricus respectively, inoculating them into MRS culture medium respectively, and culturing them at 37° C. for 24 h to obtain activated Lactobacillus plantarum, activated Lactobacillus rhamnosus and activated Lactobacillus bulgaricus;

[0076] S13, mixing black mulberry, spearmint and sea fennel uniformly to obtain a mixture A, mixing the mixture A and deionized water in a volume ratio of 1:10 and boiling, filtering to obtain a filtrate A and a filter residue A, mixing the filter residue A and deionized water in a volume ratio of 1:9 and boiling again to obtain a filtrate B and a filter residue B, combining the filtrate A and the filtrate B and concentrating to half the total volume of the filtrate A and the filtrate B to obtain a concentrated solution, and sterilizing at 121° C. for 35 min to obtain a sterilized water extract;

[0077] S20, preparing fermentation liquid: the activated Lactobacillus plantarum, the activated Lactobacillus rhamnosus and the activated Lactobacillus bulgaricus are mixed uniformly in a volume ratio of 3.25:1.5:1.5 to obtain a mixed bacterial agent, the mixed bacterial agent is added to the sterile water extract, shaken, sealed and fermented at 29° C. for 72 h to obtain a fermentation material, inactivated at 121° C. for 25 min, filtered and centrifuged to obtain a fermentation liquid;

[0078] The mixed bacterial agent accounts for 0.3% of the volume percentage of the sterilized water extract;

[0079] S30, kojic acid and deionized water are mixed evenly, and mixed solution B and mixed solution A are added in sequence, and the temperature is controlled at 30° C. and stirred evenly to obtain a finished composition.

[0080] Comparative Example 1

[0081] Compared with Example 1, the difference is that in Comparative Example 1, no black mulberry is added, but the spearmint is 6.2 parts by weight, the sea fennel is 9 parts by weight, and the other parameters and operating steps remain unchanged.

[0082] Comparative Example 2

[0083] Compared with Example 1, the difference is that in Comparative Example 2, no spearmint is added, but black mulberry is 12.1 parts by weight, and sea fennel is 3.1 parts by weight, and other parameters and operating steps remain unchanged.

[0084] Comparative Example 3

[0085] Compared with Example 1, the difference is that in Comparative Example 3, no sea fennel is added, but black mulberry is 13.5 parts by weight, spearmint is 1.7 parts by weight, and other parameters and operating steps remain unchanged.

[0086] Comparative Example 4

[0087] Compared with Example 1, the difference is that in Comparative Example 4, activated Lactobacillus plantarum is not added, and step S20 is specifically as follows:

[0088] S20, preparing fermentation liquid: the activated Lactobacillus rhamnosus and the activated Lactobacillus bulgaricus were mixed uniformly in a volume ratio of 3.5:2.5 to obtain a mixed bacterial agent, the mixed bacterial agent was added to the sterilized water extract, shaken, sealed and fermented at 28 ° C for 72 h to obtain a fermentation material, inactivated at 121 ° C for 22.5 min, filtered and centrifuged to obtain a fermentation liquid;

[0089] The mixed bacterial agent accounts for 0.4% of the volume percentage of the sterile water extract;

[0090] The remaining parameters and operating steps remain unchanged.

[0091] Comparative Example 5

[0092] Compared with Example 1, the difference is that in Comparative Example 5, activated Lactobacillus rhamnosus is not added, and step S20 is specifically as follows:

[0093] S20, preparing fermentation liquid: the activated plant lactobacillus and the activated bulgaricus lactobacillus are mixed uniformly in a volume ratio of 4:2 to obtain a mixed bacterial agent, the mixed bacterial agent is added to the sterile water extract, shaken, sealed and fermented at 28 ° C for 72 h to obtain a fermentation material, inactivated at 121 ° C for 22.5 min, filtered and centrifuged to obtain a fermentation liquid;

[0094] The mixed bacterial agent accounts for 0.4% of the volume percentage of the sterile water extract;

[0095] The remaining parameters and operating steps remain unchanged.

[0096] Comparative Example 6

[0097] Compared with Example 1, the difference is that in Comparative Example 6, activated Lactobacillus bulgaricus is not added, and step S20 is specifically as follows:

[0098] S20, preparing fermentation liquid: mixing the activated plant lactobacillus and the activated rhamnosus lactobacillus in a volume ratio of 3.5:2.5 to obtain a mixed bacterial agent, adding the mixed bacterial agent to the sterilized water extract, shaking, controlling the temperature at 28° C. and sealing and fermenting for 72 h to obtain a fermentation material, controlling the temperature at 121° C. and inactivating for 22.5 min, filtering, and centrifuging to obtain a fermentation liquid;

[0099] The mixed bacterial agent accounts for 0.4% of the volume percentage of the sterile water extract;

[0100] The remaining parameters and operating steps remain unchanged.

[0101] Test Example 1

[0102] DPPH free radical scavenging ability determination:

[0103] Weigh a certain amount of DPPH and prepare a 0.1 mg / mL DPPH solution with anhydrous ethanol. Take 2 mL of the finished composition obtained in Examples 1-3 and Comparative Examples 1-6, add 1000 g of sample solution diluted with anhydrous ethanol, add 2 mL of DPPH solution, mix well, react at room temperature for 30 minutes, and measure the absorbance Ai at 517 nm. At the same time, measure the absorbance A0 of 2 mL DPPH solution + 2 mL anhydrous ethanol and the absorbance Aj after 2 mL sample solution + 2 mL anhydrous ethanol. Repeat the measurement twice. The scavenging rate of the sample for DPPH free radicals is calculated using the following formula:

[0104] DPPH free radical scavenging rate (%) = (A0-Aj+Ai) / A0×100%;

[0105] Evaluation criteria: The antioxidant capacity can be reflected by the DPPH free radical scavenging ability test; the higher the DPPH free radical scavenging rate, the stronger the antioxidant capacity, and vice versa;

[0106] The results are shown in Table 1.

[0107] Table 1

[0108] DPPH free radical scavenging rate (%) Example 1 96.8 Example 2 96.7 Example 3 96.7 Comparative Example 1 82.3 Comparative Example 2 85.9 Comparative Example 3 84.5 Comparative Example 4 88.8 Comparative Example 5 90.1 Comparative Example 6 91.4

[0109] It can be seen from Table 1 that the finished composition prepared by the present invention has excellent antioxidant properties.

[0110] Comparative Examples 7-10

[0111] Compared with Example 1, the difference is that the weight parts of 4-butylresorcinol, kojic acid, ascorbyl tetraisopalmitate and ethylhexylglycerol in Comparative Examples 7-10 are as shown in Table 2, and the other parameters and operating steps remain unchanged.

[0112] Table 2

[0113]

[0114]

[0115] Test Example 2

[0116] (1) Determination of in vitro tyrosinase inhibition rate:

[0117] Use 10mL test tubes to set up sample tubes (T2), sample background (T1), enzyme reaction tubes (C2) and solvent background (C1). For each sample tube (T2) and enzyme reaction tube (C2) of each tested concentration, three parallel tubes are required. The finished compositions prepared in Examples 1-3 and Comparative Examples 7-10 are added as sample solutions with reference to Table 3. After being fully mixed, they are placed in a 37°C water bath and incubated for 10 minutes. 2mL of levodopa solution is added to each tube in turn, and the reaction time of each tube is controlled to be 5 minutes. The reaction solutions in each tube are immediately transferred to a cuvette, and the absorbance is measured at 475nm. The tyrosinase inhibition rate of the finished composition is calculated according to the following formula:

[0118] Tyrosinase inhibition rate (%) = 1-[(A T2 -A T1 ) / A C2 -A C1 )];

[0119] Table 3 Sample addition requirements:

[0120] Table 3

[0121]

[0122] The results are shown in Table 4.

[0123] Table 4

[0124]

[0125]

[0126] From the tyrosinase inhibition rate data in Table 4, it can be seen that the finished composition prepared by the present invention has an outstanding whitening and spot-lightening effect.

[0127] (2) Hydration and moisturizing test: Volunteers aged 35-55 years old, half male and half female, were randomly invited and divided into 7 groups, each with 12 volunteers. Each group of volunteers used the finished composition prepared by adding the finished composition prepared by Examples 1-3 and Comparative Examples 7-10 to the same essence matrix (the added amount of the finished composition in the essence was 3%).

[0128] The method of use is to evenly apply 1 mL of the finished essence to the cheek area of ​​the face and massage until it is completely absorbed. Use it once a day in the morning and evening for 28 days. During this period, no other skin care products are used in this area. Before using the product and after 28 days of use, the skin moisture content of the application area is measured using a skin moisture tester. The test of the same subject is performed by the same instrument and the same tester, thereby evaluating the moisture content of the skin before and after using the finished essence; the results are shown in Table 5.

[0129] Table 5

[0130]

[0131] It can be seen from Table 5 that the finished composition prepared by the present invention has an outstanding moisturizing effect.

[0132] In the description of the specification, the description with reference to the terms "one embodiment", "example", "specific example", etc. means that the specific features, structures, materials or characteristics described in conjunction with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representation of the above terms does not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in any one or more embodiments or examples in a suitable manner.

[0133] The above contents are merely examples and explanations of the present invention. Those skilled in the art may make various modifications or additions to the specific embodiments described or replace them in a similar manner. As long as they do not deviate from the invention or exceed the scope defined by the claims, they shall all fall within the protection scope of the present invention.

Claims

1. A whitening and spot-lightening composition containing 4-butylresorcinol, characterized in that: The whitening and spot-lightening composition specifically comprises the following raw materials in parts by weight:

2. The whitening and spot-lightening composition containing 4-butylresorcinol according to claim 1, characterized in that: The solvent comprises deionized water and butanediol in a mass ratio of 4-5:0.3-0.

4.

3. The whitening and spot-lightening composition containing 4-butylresorcinol according to claim 1, characterized in that: The whitening and spot-lightening composition further comprises Lactobacillus plantarum, Lactobacillus rhamnosus and Lactobacillus bulgaricus.

4. The whitening and spot-lightening composition containing 4-butylresorcinol according to claim 1, characterized in that: The preparation method of the whitening and spot-lightening composition containing 4-butylresorcinol comprises the following steps: S10, activating the strains and pretreating part of the raw materials to obtain activated Lactobacillus plantarum, activated Lactobacillus rhamnosus, activated Lactobacillus bulgaricus, sterilized water extract, mixed solution A and mixed solution B; S20, preparing fermentation liquid: uniformly mixing the activated Lactobacillus plantarum, the activated Lactobacillus rhamnosus and the activated Lactobacillus bulgaricus to obtain a mixed bacterial agent, adding the mixed bacterial agent to the sterilized water extract, shaking, sealing and fermenting under temperature control to obtain a fermentation material, inactivating under temperature control, filtering and centrifuging to obtain a fermentation liquid; S30, kojic acid and deionized water are mixed evenly, and mixed solution B and mixed solution A are added in sequence, and the mixture is stirred evenly under controlled temperature to obtain a finished composition.

5. The whitening and spot-lightening composition containing 4-butylresorcinol according to claim 4, characterized in that: Step S10 specifically includes: S11, 4-butylresorcinol and butanediol are mixed evenly to obtain a mixed solution A, which is set aside; ascorbyl tetraisopalmitate and ethylhexylglycerol are mixed evenly to obtain a mixed solution B, which is set aside; S12, taking 50 μL of Lactobacillus plantarum, Lactobacillus rhamnosus and Lactobacillus bulgaricus respectively, inoculating them into MRS culture medium respectively, and culturing them at a constant temperature to obtain activated Lactobacillus plantarum, activated Lactobacillus rhamnosus and activated Lactobacillus bulgaricus; S13. Evenly mix black mulberry, spearmint and sea fennel to obtain a mixture A, evenly mix the mixture A and deionized water, boil the mixture, and filter to obtain a filtrate A and a filter residue A, evenly mix the filter residue A and deionized water, boil the mixture, and obtain a filtrate B and a filter residue B, combine the filtrate A and the filtrate B, concentrate the mixture, and obtain a concentrated solution. The mixture is sterilized by temperature control to obtain a sterilized water extract.

6. The whitening and spot-lightening composition containing 4-butylresorcinol according to claim 4, characterized in that: In step S20, the volume ratio of the activated Lactobacillus plantarum, the activated Lactobacillus rhamnosus and the activated Lactobacillus bulgaricus is 3-3.5:1.5-2:1-1.5; the mixed bacterial agent accounts for 0.3-0.5% of the volume percentage of the sterile water extract.

7. The whitening and spot-lightening composition containing 4-butylresorcinol according to claim 1, characterized in that: The temperature of the temperature-controlled sealed fermentation in step S20 is 28-30°C for 72 hours; the temperature of the temperature-controlled inactivation is 121°C for 20-25 minutes.

8. The whitening and spot-lightening composition containing 4-butylresorcinol according to claim 1, characterized in that: The temperature for temperature control and uniform stirring in step S3 is 28-30°C.

9. Use of a whitening and spot-lightening composition containing 4-butylresorcinol as claimed in any one of claims 3 to 8, characterized in that: The whitening and spot-lightening composition is added into skin care products at a mass percentage of 3-4%.

10. The use of the whitening and spot-lightening composition containing 4-butylresorcinol according to claim 9, characterized in that: The skin care products include toners, essences, creams, masks and lotions.

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