Composition for relieving skin sensitivity and application thereof

Abstract

The invention relates to a composition for relieving skin sensitivity and application thereof, and belongs to the technical field of cosmetics. The composition for relieving skin sensitivity comprises a crocus sativus extract and a saussurea lappa root extract. According to the composition for relieving skin sensitivity, the crocus sativus extract and the saussurea lappa root extract have a synergistic effect, discomfort feelings such as stabbing pain and pruritus caused by lactic acid stabbing pain weakening experiments of people with sensitive skin can be remarkably relieved, good pain easing and itching relieving effects are achieved, and therefore sensitive symptoms are remarkably weakened.

Description

Technical Field

[0001] The present application belongs to the field of cosmetics, and specifically relates to a composition for soothing skin sensitivity and its application. Background Art

[0002] Sensitive skin has a high incidence rate worldwide. The causes of sensitive skin are very complex. Current studies believe that it may be related to impaired skin barrier function, increased nerve sensitivity, vasodilation, and increased expression of inflammatory factors. Sensitive skin specifically refers to a highly reactive state of the skin under physiological or pathological conditions. It is easily affected by external stimuli or environmental changes, causing subjective symptoms such as burning, stinging, itching, and tightness, which last for minutes or even hours, and often cannot tolerate ordinary cosmetics.

[0003] In recent years, cosmetics developed for sensitive skin are mainly aimed at restoring the skin's barrier function, reducing skin sensitivity and inflammatory reactions, and thus improving skin tolerance. In medicine, hormones such as glucocorticoids are used to achieve immediate anti-inflammatory, analgesic and soothing effects, but long-term use of hormone drugs may cause skin metabolic disorders, aggravate skin sensitivity, and even cause moon face and other phenomena.

[0004] Finding a hormone-free product to help people with sensitive skin resist discomfort such as skin stinging and itching and improve skin tolerance remains a technical problem that needs to be solved urgently. Summary of the invention

[0005] The present application provides a composition for soothing skin sensitivity. The composition is prepared by mixing saffron extract and azelaic acid root extract in a certain proportion, so as to have a synergistic soothing effect on sensitive skin.

[0006] This application involves the following:

[0007] 1. A composition for soothing skin sensitivity, comprising a saffron extract and a costusroot root extract in a physiologically acceptable medium.

[0008] 2. According to the composition of item 1, the mass ratio of the saffron extract to the aucklandia rosa root extract is 1:5-150:1.

[0009] 3. The composition according to item 1 or 2, wherein the saffron extract is 0.00001-6 wt % and the amurense root extract is 0.00001-6 wt % relative to the total weight of the composition.

[0010] 4. Use of the composition according to any one of items 1 to 3 in the preparation of a skin external product.

[0011] 5. Use of the composition described in any one of items 1 to 3 for relieving skin sensitivity symptoms.

[0012] 6. According to the use of item 5, the skin sensitivity symptoms include any one or more of redness, stinging, itching, burning, and tightness.

[0013] Effects of the Invention

[0014] The present application achieves a synergistic effect of soothing skin sensitivity by compounding saffron extract and aucklandia lappa root extract, and can be used to relieve sensitive symptoms such as skin redness, tingling, itching, burning, and tightness. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 .Nerve relaxation path diagram for different implementation plans; the red line represents fast movement distance, the green line represents medium-speed movement distance, and the black line represents slow movement distance.

[0016] Figure 2 . Column graph of total movement distance of zebrafish; compared with control sample 2, **p<0.01, ***p<0.001. Specific implementation methods

[0018] It should be noted that certain words are used in the specification and claims to refer to specific components. Those skilled in the art should understand that technicians may use different nouns to refer to the same component. This specification and claims do not use the difference in nouns as a way to distinguish components, but use the difference in components' functions as the criterion for distinction.

[0019] As mentioned throughout the specification and claims, "including" or "comprising" is an open term and should be interpreted as "including but not limited to". The subsequent description of the specification is a preferred embodiment of the present application, but the description is for the purpose of the general principles of the specification and is not intended to limit the scope of the present application. The scope of protection of the present application shall be determined by the attached claims. Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by ordinary technicians in the field to which the present disclosure belongs.

[0020] It should be understood that the embodiments of the present application described herein include "consisting of" and / or "consisting essentially of" embodiments. References to "about" a value or parameter herein include (and describe) variations to the value or parameter itself. For example, a description referring to "about X" includes a description of "X".

[0021] As used herein, the term "about XY" has the same meaning as "about X to about Y."

[0022] As used herein and in the appended claims, the singular forms "a", "an", and "the" include plural referents unless the context clearly dictates otherwise. It should also be noted that the claims may be drafted to exclude any optional elements. This statement is therefore intended to serve as antecedent basis for the use of exclusive terminology such as "only", "only" and the like in connection with the recitation of claim elements, or the use of a limitation of "no".

[0023] As used herein, the term "and / or" in phrases such as "A and / or B" is intended to include both A and B; A or B; A (alone); and B (alone). Similarly, as used herein, the term "and / or" in phrases such as "A, B, and / or C" is intended to include each of the following embodiments: A, B, and C; A, B, or C; A or C; A or B; B or C; A and C; A and B; B and C; A (alone); B (alone); and C (alone).

[0024] The present application discloses a composition for soothing skin sensitivity, which comprises saffron extract and azelaic acid root extract in a physiologically acceptable medium.

[0025] In some embodiments, the mass ratio of the saffron extract to the atractylodes lancea root extract is 1:5-150:1. For example, the mass ratio of the saffron extract to the atractylodes lancea root extract can be 1:5, 1:4, 1:3, 1:2, 1:1, 2:1, 3:1, 4:1, 5:1, 6:1, 7:1, 8:1, 9:1, 10:1, 11:1, 12:1, 13:1, 14:1, 15:1, 16:1, 17:1, 18:1, 19:1, 20:1, 21:1, 22:1, 23:1, 24:1, 25:1, 26:1, 27:1, 28:1, 29:1, 30:1, 31:1, 32:1, 33:1, 34:1, 35:1, 36:1 、37:1、38:1、39:1、40:1、41:1、42:1、43:1、44:1、45:1、46:1、47:1、48:1、49:1、50:1、51:1、52:1、53:1、54:1、55:1、56:1、57:1、58:1、59:1、60:1、61:1、62:1、63:1、64:1、65:1、66:1、67:1、68:1、69:1、70:1、71:1、72:1、73:1、74:1、75:1、76:1、77:1、78:1、 79:1、80:1、81:1、82:1、83:1、84:1、85:1、86:1、87:1、88:1、89:1、90:1、91:1、92:1、93:1、94:1、95:1、96:1、97:1、98:1、99:1、100:1、101:1、102:1、103:1、104:1、105:1、106:1、107:1、108:1、109:1、110:1、111:1、112:1、113:1、114:1、115:1、116:1、117: 1, 118:1, 119:1, 120:1, 121:1, 122:1, 123:1, 124:1, 125:1, 126:1, 127:1, 128:1, 129:1, 130:1, 131:1, 132:1, 133:1, 134:1, 135:1, 136:1, 137:1, 138:1, 139:1, 140:1, 141:1, 142:1, 143:1, 144:1, 145:1, 146:1, 147:1, 148:1, 149:1, 150:1, and any range therebetween.

[0026] In some embodiments, the saffron extract is 0.00001-6 wt % relative to the total weight of the composition. For example, the saffron extract is 0.00001wt%, 0.00002wt%, 0.00003wt%, 0.00004wt%, 0.00005wt%, 0.00006wt%, 0.00007wt%, 0.00008wt%, 0.00009wt%, 0.0001wt%, 0.0002wt%, 0.0003wt%, 0.0004wt%, 0.0005wt%, 0.0006wt%, 0.0007wt%, 0.0008wt%, 0.0009wt%. .006wt%, 0.007wt%, 0.008wt%, 0.009wt%, 0.01wt%, 0.02wt%, 0.03wt%, 0.04wt%, 0.05wt%, 0.06wt%, 0.07wt%, 0.08wt%, 0.09wt%, 0.1wt%, 0.2wt%, 0.3wt%, 0.4wt%, 0.5wt%, 0.6wt%, 0.7wt%, 0.8wt%, 0.9wt%, 1wt%, 1.5wt%, 2wt%, 2.5wt%, 3wt%, 3.5wt%, 4wt%, 4.5wt%, 5wt%, 5.5wt%, 6wt%, and any range therebetween.

[0027] In some embodiments, the Aucklandia lappa root extract is 0.00001-6 wt % relative to the total weight of the composition. For example, the extract of Aucklandia lappa root is 0.00001wt%, 0.00002wt%, 0.00003wt%, 0.00004wt%, 0.00005wt%, 0.00006wt%, 0.00007wt%, 0.00008wt%, 0.00009wt%, 0.0001wt%, 0.0002wt%, 0.0003wt%, 0.0004wt%, 0.0005wt%, 0.0006wt%, 0.0007wt%, 0.0008wt%, 0.0009wt%, 0.001wt%, 0.002wt%, 0.003wt%, 0.0033wt%, 0.004wt%, 0.005 %, 0.01wt%, 0.02wt%, 0.03wt%, 0.04wt%, 0.05wt%, 0.06wt%, 0.07wt%, 0.08wt%, 0.09wt%, 0.1wt%, 0.2wt%, 0.3wt%, 0.4wt%, 0.5wt%, 0.6wt%, 0.7wt%, 0.8wt%, 0.9wt%, 1wt%, 1.5wt%, 2wt%, 2.5wt%, 3wt%, 3.5wt%, 4wt%, 4.5wt%, 5wt%, 5.5wt%, 6wt%, and any range therebetween.

[0028] In some embodiments, the content of flavonoids in the saffron extract is greater than 5%, for example, the content of flavonoids in the saffron extract is 5.1%, 5.2%, 5.3%, 5.4%, 5.5%, 5.6%, 5.7%, 5.8%, 5.9%, 6.0%, 6.1%, 6.2%, 6.3%, 6.4%, 6.5%, 6.6%, 6.7%, 6.8%, 6.9%, 7.0%, 7.1%, 7.2%, 7.3%, 7.4%, 7.5%, 7.6%, 7.7%, 7.8%, 7.9%, 8.0%, 8.1%, 8.2%, 8.3%, 8.4%, 8.5%, 8.6%, 8.7%, 8.8%, 8.9%, 8.0%, 9.1%, 9.2%, 9.3%, 9.4%, 9.5%, 9.6%, 9.7%, 9.8%, 9.9%, 9.0%, 9.1%, 9.2%, 9.3%, 9.4%, 9.5%, 9.7%, 9.8%, 9.9%, 9.0%, 9.1%, 9.2%, 9.3%, 9.4%, 9.6%, 9.7%, 9.8%, 9.9%, 9.0%, 9.1%, 9.2%, 9.3%, 9.4%, 9.5%, 9.6%, 9.7%, 9.8%, 9.9%, 9.0%, 9.1%, 9.2%, 9.3 %, 9.4%, 9.5%, 9.6%, 9.7%, 9.8%, 9.9%, 10.0%, 20.0%, 30.0%, 40.0%, 50.0%, 60.0%, 70.0%, 80.0%, 90.0%, and any range therebetween; preferably, the content of total carbohydrates is >70%, for example, the content of total carbohydrates is 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%, 91%, 92%, 93%, 94%, 95%, 96%, 97%, 98%, 99%, and any range therebetween.

[0029] In some embodiments, the saffron extract can be commercially available or extracted by itself.

[0030] In some specific embodiments, the saffron extract is obtained by self-extraction, for example, by drying saffron at low temperature to make powder, extracting with ethanol solution, purifying and drying.

[0031] In some embodiments, the content of total lactones in the Aucklandia lappa root extract is ≥20%, for example, the content of total lactones in the Aucklandia lappa root extract is 20%, 21%, 22%, 23%, 24%, 25%, 26%, 27%, 28%, 29%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, and any range therebetween.

[0032] In some embodiments, the total lactones include costunolide and dehydrocostuslactone.

[0033] In some embodiments, the Aucklandia lappa root extract can be commercially available or extracted by itself.

[0034] In some specific embodiments, the Aucklandia lappa root extract is obtained by self-extraction, for example, by making the Aucklandia lappa root into powder, extracting it with ethanol solution, purifying it, and drying it.

[0035] In some specific embodiments, the crocus flower extract and the aucklandia odorifera root extract may be added in the form of powder or diluted solution.

[0036] In some specific embodiments, when the saffron extract is added in the form of a diluted solution, the content of the saffron extract in the solution can be 0.001wt%-99.999wt%, preferably 0.1wt%-1wt%, for example, the content of the saffron extract in the solution is 0.001wt%, 0.002wt%, 0.003wt%, 0.0033wt%, 0.004wt%, 0.005wt%, 0.006wt%, 0.007wt%, 0.008wt%, 0.009wt%, 0.01wt%, 0.02wt%, 0.03wt%, 0.04wt%, 0.05wt%, 0.06wt%, 0.07wt%, 0.08wt%, 0.09wt%, 0.1 ... %, 15wt%, 0.2wt%, 0.25wt%, 0.3wt%, 0.35wt%, 0.4wt%, 0.45wt%, 0.5wt%, 0.55wt%, 0.6wt%, 0.65wt%, 0.7wt%, 0.75wt%, 0.8wt%, 0.85wt%, 0.9wt%, 0.95wt%, 1wt%, 1.5wt%, 2wt%, 2.5wt%, 3wt%, 3.5wt%, 4wt%, 4.5wt%, 5wt%, 5.5wt%, 6wt%, 7wt%, 8wt%, 9wt%, 10wt%, 20wt%, 30wt%, 40wt%, 50wt%, 60wt%, 70wt%, 80wt%, 90wt%, and any range therebetween.

[0037] In some embodiments, the solution containing saffron extract is purchased from SEQENS company Sens'flower TM The content of saffron extract in the product is 0.55wt%, and the other components are solvents.

[0038] In some specific embodiments, when the cloud costus root extract is added in the form of a diluted solution, the content of the cloud costus root extract in the solution can be 0.001wt%-99.999wt%, preferably 0.05wt%-0.5wt%. For example, the content of the cloud costus root extract in the solution is 0.001wt%, 0.002wt%, 0.003wt%, 0.0033wt%, 0.004wt%, 0.005wt%, 0.006wt%, 0.007wt%, 0.008wt%, 0.009wt%, 0.01wt%, 0.02wt%, 0.03wt%, 0.04wt%, 0.05wt%, 0.06wt%, 0.07wt%, 0.08wt%, 0.09wt%, 0.1wt%, %, 0.15wt%, 0.2wt%, 0.25wt%, 0.3wt%, 0.35wt%, 0.4wt%, 0.45wt%, 0.5wt%, 0.55wt%, 0.6wt%, 0.65wt%, 0.7wt%, 0.75wt%, 0.8wt%, 0.85wt%, 0.9wt%, 0.95wt%, 1wt%, 1.5wt%, 2wt%, 2.5wt%, 3wt%, 3.5wt%, 4wt%, 4.5wt%, 5wt%, 5.5wt%, 6wt%, 7wt%, 8wt%, 9wt%, 10wt%, 20wt%, 30wt%, 40wt%, 50wt%, 60wt%, 70wt%, 80wt%, 90wt%, and any range therebetween.

[0039] In some specific embodiments, the solution containing the root extract of Aucklandia lappa is purchased from Inger Biotechnology. TM The content of the extract of Aucklandia lappa root in the product is 0.1wt%, and the other components are solvents.

[0040] In some specific embodiments, when the saffron extract and the cloud costus root extract are added in the form of a diluted solution, the mass ratio of the solution containing the saffron extract to the solution containing the cloud costus root extract can be 1:30-30:1. For example, the mass ratio of the solution containing the saffron extract to the solution containing the cloud costus root extract can be 1:30, 1:29, 1:28, 1:27, 1:26, 1:25, 1:24, 1:23, 1:22, 1:21, 1:20, 1:19, 1:18, 1:17, 1:16, 1:15, 1:14, 1:13, 1:12, 1:11, 1:10, 1:9, 1:8, 1:7, 1:6, 1:5, 1:4, 1:3, 1:2, 1:1, 5:1, 6:1, 7:1, 8:1, 9:1, 10:1, 11:1, 12:1, 13:1, 14:1, 15:1, 16:1, 17:1, 18:1, 19:1, 20:1, 21:1, 22:1, 23:1, 24:1, 25:1, 26:1, 27:1, 28:1, 29:1, 30:1.

[0041] In some more specific embodiments, Sens'flower TM He Yunyi TM The mass ratio can be 1:30-30:1. For example, it can be 1:30, 1:29, 1:28, 1:27, 1:26, 1:25, 1:24, 1:23, 1:22, 1:21, 1:20, 1:19, 1:18, 1:17, 1:16, 1:15, 1:14, 1:13, 1:12, 1:11, 1:10, 1:9, 1:8, 1:7, 1:6, 1:5, 1:4, 1:1 :3, 1:2, 1:1, 5:1, 6:1, 7:1, 8:1, 9:1, 10:1, 11:1, 12:1, 13:1, 14:1, 15:1, 16:1, 17:1, 18:1, 19:1, 20:1, 21:1, 22:1, 23:1, 24:1, 25:1, 26:1, 27:1, 28:1, 29:1, 30:1.

[0042] According to the present application, the physiologically acceptable medium is in particular a cosmetically acceptable medium and may be non-aqueous or aqueous. It may therefore comprise an aqueous phase and / or an oily phase.

[0043] Among the physiologically acceptable media that can be used in the present application, their composition, their amount, their preparation method and application method can be selected by those skilled in the art based on their common sense, depending on the type of composition required. The types of media that can be used include solvents, excipients, preservatives, antibacterial agents, anti-inflammatory agents, moisturizers, antioxidants, propellants, whitening agents, thickeners, suspending agents, surfactants, acid-base regulators, complexing agents, pearlescent agents, astringents, fragrances, etc.

[0044] In some embodiments, the preservatives include but are not limited to any one or more of p-hydroxyacetophenone and 1,2-hexanediol; the moisturizers include but are not limited to any one or more of butylene glycol, glycerin, 1,2-pentanediol, tetrahydromethylpyrimidine carboxylic acid, hydrolyzed sodium hyaluronate, trehalose, Tremella fuciformis fruiting body extract, ethylhexylglycerin, sodium γ-polyglutamate, avocado oil, squalane and betaine.

[0045] In some embodiments, the composition includes crocus flower extract, costusroot root extract, a preservative, a humectant, and a solvent.

[0046] In some embodiments, the composition includes crocus flower extract, azelaic acid root extract, p-hydroxyacetophenone, 1,2-hexanediol, butylene glycol, glycerin, and water.

[0047] In some embodiments, the composition includes 0.00001-6 parts of saffron extract, 0.00001-6 parts of amurense root extract, 0.5-4 parts of preservative, 1.5-12 parts of moisturizer, and 70-99 parts of solvent.

[0048] In some embodiments, the composition includes 0.00001-6 parts of saffron extract, 0.00001-6 parts of amurense root extract, 0.5-2 parts of p-hydroxyacetophenone, 0.5-2 parts of 1,2-hexanediol, 1-6 parts of butylene glycol, 1-6 parts of glycerin, and 70-99 parts of water.

[0049] The preparation method of the composition for soothing skin sensitivity of the present application is not particularly limited, and for example, it can be prepared by the following steps:

[0050] The saffron extract and the aucklandia rosa root extract are dissolved in water according to a certain proportion to obtain the product.

[0051] In some embodiments, the preservative, moisturizer, saffron extract, and aucklandia lappa root extract are dissolved in water according to proportion.

[0052] In some embodiments, the preservative and moisturizer are dissolved in water in proportion and mixed evenly, and then the saffron extract and the amurense root extract are added and dissolved in proportion to obtain the product.

[0053] In some embodiments, the temperature of the water during dissolution is 30-50°C, for example, the temperature of the water is 30°C, 35°C, 40°C, 45°C, 50°C, and any range therebetween.

[0054] In some embodiments, the water used for dissolution is sterile water, which can be obtained by commonly used means in the art, or the water can be subjected to sterilization treatment including the following steps before dissolution: the water temperature is brought to 80-85°C and kept warm for 25-35 minutes. In some embodiments, to achieve sterilization, the water temperature is heated to 80-85°C, exemplarily 80°C, 81°C, 82°C, 83°C, 84°C, 85°C or 80-85°C, and any range therebetween. In some embodiments, to achieve sterilization, the insulation time at 80-85°C is 25-35 minutes, exemplarily 25 minutes, 28 minutes, 30 minutes, 33 minutes, 35 minutes, and any range therebetween.

[0055] In some embodiments, the method for preparing a composition for soothing skin sensitivity comprises the following steps:

[0056] 1) Stir the deionized water to raise the temperature to 80-85°C, keep it at this temperature for 25-35 minutes, and then cool it down to 30-50°C;

[0057] 2) Pour the preservative and moisturizer into the container according to the proportion and mix and stir until they are completely dissolved;

[0058] 3) adding saffron extract and costusroot root extract into a container according to a certain proportion, stirring evenly, and preparing a composition for soothing skin sensitivity.

[0059] The present application also provides the use of the above-mentioned composition in the preparation of external skin products.

[0060] The present application also provides the use of the above-mentioned composition in alleviating skin sensitivity symptoms.

[0061] In some embodiments, the skin sensitivity symptoms include any one or more of redness, stinging, itching, burning, and tightness.

[0062] The saffron extract and the amurense root extract in the composition for soothing skin sensitivity of the present application produce a nerve soothing effect after compounding. It was used to resist the discomfort after lactic acid stimulation. The results showed that after two weeks, the inhibition rate of skin lactic acid discomfort reached more than 30%, which was significantly higher than the inhibition rate of saffron extract or amurense root extract. It can be seen that the saffron extract and the amurense root extract in the composition of the present application work synergistically and have a significantly excellent effect of soothing skin sensitivity. Example

[0063] The specific embodiments of the present application will be described in more detail below with reference to the accompanying drawings. Although the specific embodiments of the present application are shown in the accompanying drawings, it should be understood that the present application can be implemented in various forms and should not be limited by the embodiments set forth herein. On the contrary, these embodiments are provided in order to enable a more thorough understanding of the present application and to fully convey the scope of the present application to those skilled in the art.

[0064] The present application provides a general and / or specific description of the materials and test methods used in the experiments. In the following examples, unless otherwise specified, % means wt%, i.e., weight percentage. The reagents or instruments used without indicating the manufacturer are all conventional reagent products that can be obtained commercially.

[0065] Test Example 1: Evaluation of the neurosoothing effect of zebrafish

[0066] This example introduces a zebrafish neurosoothing efficacy test, applies behavioral analysis to detect the total movement distance of zebrafish, and objectively evaluates the analgesic and soothing efficacy of the sample.

[0067] (1) Test principle:

[0068] Although glacial acetic acid is a weak acid, it has strong piercing and corrosive properties. When the skin comes into contact with glacial acetic acid, it will feel stinging and burning. We selected zebrafish whose swimming ability has not yet fully developed and used glacial acetic acid to stimulate the zebrafish to cause inflammatory pain over a larger area and for a longer period of time. The more movement tracks there are, the more severe the pain is. We used an applied behavioral analyzer to detect the total movement distance of the zebrafish to evaluate whether the sample has a neurosoothing effect.

[0069] (2) Test method:

[0070] Zebrafish 2 days after fertilization (2dpf) were divided into ten groups and randomly placed in 6-well plates. The samples were given in water, and normal control group, model control group, positive control group and test group were set up respectively. The normal control group was not treated, and the positive control group and the test group were given positive drugs (kava extract, specifically water as solvent to dissolve kava extract) and test samples, respectively, and treated at 28°C for 24h. Ten zebrafish were randomly selected from each group and transferred to a 96-well plate. Glacial acetic acid was given to the model control group, positive control group and test group at the same time, and the movement trajectory of the zebrafish within 0.5h was immediately measured by a behavior analyzer, and the total movement distance (D) of the zebrafish was analyzed and collected, and the formula was used to determine whether the sample had a soothing effect.

[0071] (3) Evaluation criteria:

[0072] Nerve soothing effect (%) = (D (model control group) – D (sample group)) / (D (model control group) – D (normal control group)) * 100%;

[0073] Determination of neurosoothing efficacy: Compare whether the neurosoothing efficacy of the samples is significantly different from that of the model control group (P < 0.05). If so, the samples are determined to have neurosoothing efficacy under the experimental conditions.

[0074] (4) Test subjects and other information:

[0075] Experimental system: Wild-type AB strain zebrafish.

[0076] Zebrafish age: 2 days post fertilization (2 dpf).

[0077] The sample size of each experimental group was 15 (N=10).

[0078] Adult fish rearing and breeding methods: follow the standard rearing and breeding methods of our laboratory, in line with the requirements of the international AAALAC certification (certification number: 001458).

[0079] (5) Test samples

[0080] Sens'flower TM : Purchased from SEQENS, the saffron extract content is 0.55%; the remaining components are solvents, specifically 1,3-propylene glycol (80-85%) and water (balance). The total carbohydrate content of the saffron extract is 78.2%, and the total flavonoid content is 6.4%.

[0081] Yunyi TM : Purchased from Inge Biotechnology, the content of cloud costus root extract is 0.1%, and the remaining components are solvents, specifically butylene glycol (80%), PEG-40 hydrogenated castor oil (3%) and water (16.9%). The content of total lactones (calculated as costus lactone and dehydrocostus lactone) in cloud costus root extract is 21%.

[0082] Kava extract: purchased from Shaanxi Xintianyu, distilled water and other ingredients.

[0083] Example

[0084] Example 1-Example 3, Comparative Example 6-Comparative Example 7

[0085] Deionized water is used as a solvent, and saffron extract and amurense root extract are added respectively and mixed evenly to obtain the product. The contents of saffron extract and amurense root extract are shown in Table 1.

[0086] Comparative Example

[0087] Comparative Example 1-Comparative Example 5

[0088] Comparative Example 1 is the normal control group mentioned above; Comparative Example 2 is the model control group mentioned above; and Comparative Example 3 is the positive control group mentioned above. The experimental parameters of Comparative Examples 1 to 3 are shown in Table 1.

[0089] The only difference between Comparative Example 4 and Example 1 is that the prepared composition for soothing skin sensitivity contains only saffron extract; the only difference between Comparative Example 5 and Example 1 is that the prepared composition for soothing skin sensitivity contains only Aucklandia lappa root extract; the specific experimental parameters of Comparative Example 4 and Comparative Example 5 are shown in Table 1.

[0090] Table 1 Component ratios and experimental parameters of the compositions in different embodiments

[0091]

[0092] Zebrafish neural relaxation pathways Figure 1 As shown in Table 2, the total movement distance of zebrafish Figure 2 The neurosoothing effects of zebrafish are shown in Table 2.

[0093] From Table 2 and Figure 2 It can be seen that under the experimental conditions, the total movement distance of zebrafish in the model control group was significantly increased compared with the normal control group, and the total movement distance of zebrafish in the positive control group was significantly reduced compared with the model control group, indicating that the neurosoothing model was successfully established.

[0094] Under the conditions of this experiment, the use of saffron extract (Comparative Example 4) or cloud costus root extract (Comparative Example 5) alone did not significantly reduce the total movement distance of zebrafish after receiving glacial acetic acid stimulation compared with the model control group, and did not have a significant soothing effect.

[0095] An unexpected effect was observed after compounding saffron extract and costus root extract: when the compounding ratio of saffron extract: costus root extract was within the range of 1:5-100:1 (refer to Example 1, Example 2 and Example 3), the compounding of the two had a neurosoothing effect compared with the model control group (Comparative Example 2).

[0096] Table 2 Zebrafish nerve soothing effect (%)

[0097]

[0098] (P>0.05 is ns; P<0.05 is *; P<0.01 is **; P less than 0.001 is ***)

[0099] Test Example 2: Lactic acid stinging test

[0100] (1) Test principle:

[0101] Lactic acid can sensitively identify sensitive skin caused by damaged skin barrier. Lactic acid has the advantage of small molecular weight, and it is very easy to penetrate through the skin after the skin barrier is damaged, stimulating the unmyelinated C nerves in the dermis, and then causing discomfort reactions such as tingling, itching, and burning.

[0102] The lactic acid sting test (LAST) is one of the semi-subjective methods for evaluating sensitive skin mentioned in the "Chinese Expert Consensus on the Diagnosis and Treatment of Sensitive Skin". It is also the most recognized and widely used method for evaluating sensitive skin.

[0103] (2) Test method:

[0104] 1) Lactic acid sting test method and screening of lactic acid sting positive people:

[0105] 50 μL of 10% lactic acid or distilled water was dropped on a filter paper with a diameter of 8 mm and placed on the nasolabial grooves of the subjects. The subjects were asked to evaluate the discomfort of the test site at 30 seconds, 2.5 minutes and 5 minutes respectively according to the four-point system. The score was scored according to the four-point system (0 for no feeling, 1 for mild, 2 for moderate, and 3 for severe). The sum of the scores of 2.5 minutes and 5 minutes was ≥ 3 points, which was considered positive for lactic acid stinging, that is, people with sensitive skin.

[0106] 2) Long-term use of lactic acid sting test:

[0107] Subjects who met the inclusion criteria underwent a long-term use trial 3 days after screening, and lactic acid stinging evaluation was performed before use (baseline value) and 2 weeks after use, including evaluation of stinging, burning and itching.

[0108] Tingling: This sensation may be similar to the prick of a needle or a slight electric shock. It may be brief or lasting, but it usually feels sharp and sudden.

[0109] Burning sensation: This sensation may be more like the burning sensation of skin touching a hot object or getting a sunburn. The subject may feel a constant sensation of heat on the surface of the skin.

[0110] Itch: This sensation is usually a scratching sensation and can be mild or intense. Itch may cause the subject to unconsciously want to touch or scratch the area.

[0111] During the experiment, we provided detailed instructions and grading standards, and the subjects were asked to accurately describe each feeling they felt, and quantify the intensity of the feeling using the lactate scale (i.e., four-point method) based on their true feelings. Among them:

[0112] 0 points, no tingling, itching, or burning sensation;

[0113] 1 point, mild tingling, itching, burning sensation (slightly felt);

[0114] 2 points, obvious tingling, itching, and burning sensation (relatively strong sensation);

[0115] 3 points, moderate tingling, itching, and burning sensation (strong sensation, but within an acceptable range).

[0116] Discomfort degree score = (30s+2.5min+5min lactic acid side all sensory scores) - (30s+2.5min+5min distilled water side all sensory scores);

[0117] Discomfort feeling suppression rate: E = (basic value - discomfort level score after 2 weeks) / basic value * 100%.

[0118] (3) Test population and test time:

[0119] Test subjects: 210 subjects, 30 subjects / group, healthy women, aged 22 to 60 years old, average (41.39±7.73) years old, in line with the inclusion and exclusion criteria of the 2015 "Technical Specifications for Safety of Cosmetics". All subjects were fully aware of the entire test process before participating in the test and voluntarily signed the informed consent. Inclusion criteria: All subjects were positive for lactic acid stinging during the first visit screening; no other skin diseases or systemic diseases; subjects did not use other facial care products during the trial and avoided excessive sunlight exposure. According to the lactic acid stinging screening results, the subjects were randomly divided into groups and used different compositions to relieve skin sensitivity. The subjects used the products once in the morning and evening for a cumulative use of 2 weeks.

[0120] (4) Test samples:

[0121] Sens'flower TM : Purchased from SEQENS, the saffron extract content is 0.55%; the remaining components are solvents, specifically 1,3-propylene glycol (80-85%) and water (balance). The total carbohydrate content of the saffron extract is 78.2%, and the total flavonoid content is 6.4%;

[0122] Yunyi TM: Purchased from Inge Biotechnology, the content of cloud costus root extract is 0.1%, and the remaining components are solvents, specifically butylene glycol (80%), PEG-40 hydrogenated castor oil (3%) and water (16.9%). The content of total lactones (calculated as costus lactone and dehydrocostus lactone) in cloud costus root extract is 21%.

[0123] Example

[0124] Example A-Example B

[0125] Preparation method of the composition with anti-allergic and soothing effects:

[0126] 1) Add deionized water into the production tank in proportion, stir and heat to 80°C, keep warm for 30 minutes, then cool to 40°C;

[0127] 2) Pour p-hydroxyacetophenone, glycerol, butylene glycol and 1,2-hexanediol into a container in proportion and mix and stir until they are completely dissolved, then add them into the production tank and stir evenly;

[0128] 3) adding saffron extract and amurense root extract into a production tank in proportion, stirring evenly, filtering the material, and preparing a composition for soothing skin sensitivity.

[0129] Referring to the above method, the compositions for soothing skin sensitivity of Example A-Example B were prepared respectively, and a lactic acid stinging test was performed; the specific components of the compositions for soothing skin sensitivity of Example A-Example B are shown in Table 3, and the results of the lactic acid stinging test are shown in Table 4.

[0130] Comparative Example

[0131] Comparative Example A-Comparative Example B

[0132] The only difference between Comparative Example A and Example A is that the prepared composition for soothing skin sensitivity contains only saffron extract; the only difference between Comparative Example B and Example A is that the prepared composition for soothing skin sensitivity contains only cloud costus root extract; the specific components of the compositions for soothing skin sensitivity of Comparative Example A and Comparative Example B are shown in Table 3, and the results of the lactic acid stinging test are shown in Table 4.

[0133] Table 3 Components and ratios of the composition for soothing skin sensitivity in different embodiments

[0134]

[0135] The composition provided in the present application, when the saffron extract and the atractylodes root extract are mixed together in proportion, has a more significant inhibition rate of discomfort caused by lactic acid stimulation; the result shows that the composition reduces the subject's perception of skin sensitivity symptoms after lactic acid stimulation, especially reduces the symptoms of stinging and itching. As shown in Table 4, there is a significant difference in the combined effect of Example A-Example B, which is far greater than the sum of the use of saffron extract or atractylodes root extract alone, indicating that the combination of the two synergistically reduces the discomfort after lactic acid stimulation and has a synergistic effect of soothing skin sensitivity.

[0136] Table 4 Uncomfortable feeling suppression rate (%)

[0137]

[0138] (P>0.05 is ns; P<0.05 is *; P<0.01 is **; P less than 0.001 is ***).

Claims

1. A composition for soothing skin sensitivity, comprising a saffron extract and a costusroot root extract in a physiologically acceptable medium.

2. The composition according to claim 1, wherein the mass ratio of the saffron extract to the aucklandia rosaefolia root extract is 1:5-150:

1.

3. The composition according to claim 1 or 2, wherein the saffron extract is 0.00001-6 wt% and the amurense root extract is 0.00001-6 wt% relative to the total weight of the composition.

4. Use of the composition according to any one of claims 1 to 3 in the preparation of external skin products.

5. Use of the composition according to any one of claims 1 to 3 in alleviating skin sensitivity symptoms.

6. The use according to claim 5, wherein the skin sensitivity symptoms include any one or more of redness, stinging, itching, burning, and tightness.

Images