Colla corii asini capable of maintaining beauty, keeping young and reducing color spots and preparation method thereof

Abstract

The invention discloses beauty maintaining, young keeping and spot reducing donkey-hide gelatin and a preparation method thereof, and relates to the technical field of donkey-hide gelatin preparation, the donkey-hide gelatin comprises the following raw materials by weight: 80-150 parts of donkey hide, 30-40 parts of red date powder, 10-20 parts of Chinese yam powder, 10-20 parts of rose powder, 10-15 parts of olive oil, 5-15 parts of rock sugar, 5-10 parts of an angelica sinensis extract, 5-10 parts of coix seed powder, and 3-8 parts of yellow wine. The invention creatively finds that the donkey hide, the olive oil and the angelica sinensis generate a compounding relationship, and an excellent technical effect is achieved. Moreover, the donkey-hide gelatin does not contain any additive, achieves the effects of maintaining beauty and keeping young from the mechanism of traditional Chinese medicine, and is safe and free of side effects to the body.

Description

Technical Field

[0001] The present invention relates to the technical field of donkey-hide gelatin preparation, and more specifically to donkey-hide gelatin for beautifying and reducing spots and a preparation method thereof. Background Art

[0002] At present, with the improvement of people's quality of life, on the basis of solving the problem of food and clothing, people pay more attention to their external image and health, and a series of beauty and skin care products have emerged. However, most of the beauty and skin care products on the market are cosmetics. Cosmetics are mostly industrial products, which can only temporarily moisturize the skin and lighten spots. In the long run, they will form dependence and are not suitable for long-term use.

[0003] Donkey hide gelatin originated from the Qin and Han Dynasties, and has a history of more than 2,000 years. It is a traditional tonic and blood-tonifying top-grade product. Donkey hide is the main raw material, and the auxiliary materials are rice wine, rock sugar, and soybean oil. Donkey hide gelatin is native to the ancient Dong'e County of Shandong Province (today's Gu'ejing, Acheng Town, Yanggu County). The best ones are amber in color, transparent, and odorless. It has the functions of nourishing blood and yin, moistening dryness, and stopping bleeding. It is used for blood deficiency, sallow complexion, dizziness, palpitations, restlessness, insomnia, and dry cough.

[0004] Existing donkey-hide gelatin generally uses fresh donkey skin or dried donkey skin as raw materials, and uses crystal sugar or xylitol, soybean oil, and rice wine as auxiliary materials. Fresh donkey skin or dried donkey skin is soaked, split, blanched, peeled, centrifuged, filtered, and concentrated, and then crystal sugar or xylitol, soybean oil, and rice wine are added. After boiling, cooling, molding, slicing, and packaging, edible donkey-hide gelatin is made. Due to the simple composition of raw materials, its functions are relatively simple, and if consumed every day, it will cause blood viscosity and easily cause cardiovascular diseases. If the formula of donkey-hide gelatin can be improved, without changing its palatability and original value, the effects of beauty and light spots can be increased, and medicine and food have the same origin, and it is suitable for all ages, then the wide applicability of donkey-hide gelatin will be greatly improved.

[0005] Therefore, how to provide a new donkey-hide gelatin formula that has the effect of beautifying, nourishing the skin and lightening spots is a technical problem that technical personnel in this field urgently need to solve. Summary of the invention

[0006] In view of this, the present invention is proposed.

[0007] One of the purposes of the present invention is to provide a kind of donkey-hide gelatin.

[0008] The second object of the present invention is to provide a method for preparing the above-mentioned donkey-hide gelatin.

[0009] The third object of the present invention is to provide the uses and effects of the above-mentioned donkey-hide gelatin.

[0010] In order to achieve the above object, the present invention adopts the following technical solution:

[0011] On the one hand, an embodiment of the present invention provides donkey-hide gelatin for beautifying and reducing pigmentation. The donkey-hide gelatin comprises the following raw materials in parts by weight: 80-150 parts of donkey skin, 30-40 parts of red date powder, 10-20 parts of yam powder, 10-20 parts of rose pollen, 10-15 parts of olive oil, 5-15 parts of crystal sugar, 5-10 parts of angelica extract, 5-10 parts of coix seed powder and 3-8 parts of rice wine.

[0012] In a preferred embodiment, the donkey-hide gelatin comprises the following raw materials in optimal weight proportions: 100 parts of donkey skin, 36 parts of red date powder, 12 parts of yam powder, 17 parts of rose pollen, 13 parts of olive oil, 10 parts of crystal sugar, 6 parts of angelica extract, 6 parts of coix seed powder and 5 parts of rice wine.

[0013] The second aspect of the embodiment of the present invention also provides a method for preparing the donkey-hide gelatin, the process is as follows:

[0014] 1) preparing red date powder, yam powder, rose pollen, angelica extract, and coix seed powder respectively, and weighing them according to the amounts, and setting aside;

[0015] 2) Pre-treat the donkey hide after picking and refining to obtain wet hide;

[0016] 3) the wet skin obtained in step 2) is mixed with water in a mass volume ratio of 1kg:0.5-0.7L and put into a steaming ball, and steam is dynamically extracted for 1-2h at 0.12-0.15mpa, and static extraction is performed for 5-6h to obtain juice; then water is added in a mass volume ratio of 1kg:0.5-0.7L of wet skin to water, and steam is dynamically extracted for 1-2h at 0.12-0.15mpa, and the process of adding water and steam is repeated until the wet skin is completely hydrolyzed and melted; to obtain gum juice;

[0017] 4) The gelatin obtained in step 3) is initially concentrated, foamed, concentrated again, foamed, water is added, concentrated, and foamed, and the process of adding water, concentrating, and foaming is repeated until the gelatin is beaded, and red date powder, yam, rose pollen, olive oil, crystal sugar, angelica extract, coix seed powder and rice wine are added, and the gelatin is further concentrated to a thick paste, gelled, cut, dried, cut, wiped, sterilized, and packaged to obtain the finished donkey-hide gelatin.

[0018] In a preferred embodiment, in step 1), the preparation process of red date powder, yam powder, rose pollen and coix seed powder is as follows:

[0019] Red dates, yam, rose and coix seeds are taken respectively, washed, freeze-dried at -20 to -40°C, ultrafinely crushed, sieved and sterilized to obtain red date powder, yam powder, rose pollen and coix seeds powder with a mesh size of 700-800.

[0020] In a preferred embodiment, the preparation process of the angelica extract in step 1) is as follows: the angelica is dried and crushed, passed through a 40-60 mesh sieve, and the angelica powder is added to the ethanol aqueous solution and soaked according to the mass volume ratio of the angelica powder to the ethanol aqueous solution of m:v=1kg:8-12L, refluxed for 2-4 times, and the extracts are combined;

[0021] In a more preferred embodiment, the volume concentration of ethanol in the ethanol aqueous solution is 70%-90%; the soaking time is 20-40 minutes; and the extraction time is 1-2 hours per time.

[0022] In a preferred embodiment, in step 2), the pretreatment is to sequentially soak, shave, cut, wash and blanch the donkey hide after picking;

[0023] In a more preferred embodiment, the soaking is performed by adding water for 5 to 7 days; the amount of water added is preferably such that the surface of the water covers the donkey skin;

[0024] The skinning is to cut the donkey skin into pieces with a side length of 30-35 cm;

[0025] Washing the leather is to place the leather in clean water and wash it until there is no impurity on the surface;

[0026] The blanching process comprises adding sodium carbonate at 0.5% of the weight of the leather block, then adding water at a mass volume ratio of the leather block to water of 1kg:0.5L, and blanching the leather with steam for 30-40 minutes.

[0027] In a preferred embodiment, in step 4), the preliminary concentration is to pump the gum juice into a concentrator and concentrate it to a relative density of 1.08-1.09, and then pump it into a container and concentrate it again;

[0028] The foaming step is to skim off the foam and impurities on the surface of the glue;

[0029] The re-concentration is carried out at 30-40° C. and a vacuum degree of -0.06 to -0.08 MPa for 1-2 h.

[0030] In a preferred embodiment, in step 4), the gel is first condensed at 2-7°C for 10-12h, and then condensed at 0-2°C for 1-2h;

[0031] The drying of the glue is carried out at a temperature of 16-25°C and a humidity of 55%-70% for 44-62 days until the moisture content is ≤15%;

[0032] The sterilization is carried out at 30-40°C and under ultraviolet radiation for 25-35 minutes.

[0033] The third aspect of the embodiment of the present invention provides the use of the donkey-hide gelatin, which is:

[0034] 1) Assist in beauty and skin care;

[0035] 2) Used in conjunction with external beauty products / skin care products, for internal or external application, to reduce pigmentation.

[0036] Furthermore, the use is for preparing external medicines or skin care products with beauty or skin care effects.

[0037] Furthermore, the external-use medicine includes but is not limited to acne cream; the skin care product includes but is not limited to facial mask.

[0038] It can be known from the above technical scheme that the technical effect achieved by the present invention is: the present invention unexpectedly discovered during the experiment that donkey skin, olive oil and angelica act synergistically. The inventor conducted a single variable experiment, replacing olive oil with soybean oil or walnut oil, or removing or replacing angelica, and found that the prepared donkey-hide gelatin paste was not satisfactory in terms of taste or effect evaluation from volunteers. Therefore, the present invention creatively discovered the synergistic effect between donkey skin, olive oil and angelica, and achieved unexpected technical effects. Moreover, the donkey-hide gelatin of the present invention does not have any additives, achieves the effect of beauty and skin care from the mechanism of traditional Chinese medicine, and is safe and has no side effects on the body. DETAILED DESCRIPTION

[0039] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0040] In the embodiment of the present invention, the soybean oil or walnut oil in the prior art is discarded and replaced with olive oil. Olive oil is rich in squalene and essential fatty acids with excellent affinity to the skin, is quickly absorbed, and effectively maintains skin elasticity and moisture. Moreover, the rich monounsaturated fatty acids and vitamins E, K, A, D, etc. and phenolic antioxidants contained in olive oil can eliminate facial wrinkles, prevent skin aging, and have the effects of skin care and hair care and prevention and treatment of chapped hands and feet. It is an "edible" beauty and skin care product. In addition, applying olive oil to the skin can resist ultraviolet rays and prevent skin cancer.

[0041] At the same time, the embodiment of the present invention adds angelica to donkey-hide gelatin. Angelica is a Chinese medicine with the same origin as medicine and food. It belongs to the liver, heart and spleen meridians, and enters the Shaoyin meridian of the hand, Taiyin meridian of the foot, and Jueyin meridian. Angelica tastes sweet and heavy, so it can only replenish blood. Its qi is light and pungent, so it can also promote blood circulation, and it can promote blood circulation while replenishing, and it can promote blood circulation while replenishing. It is an important medicine for blood. Therefore, it can not only replenish blood, but also promote blood circulation, and can both dredge menstruation and activate collaterals; red dates belong to the spleen and stomach meridians, replenish the middle qi, and replenish blood and nourish blood; yam belongs to the spleen and stomach meridians, strengthens the spleen and harmonizes the stomach; coix seeds remove dampness, belong to the liver and spleen meridians; rose belongs to the liver and spleen meridians, strengthens the liver and nourishes the stomach, promotes blood circulation and regulates menstruation, moisturizes the intestines and relieves constipation, and relieves depression and calms the mind.

[0042] In addition, if the qi and blood are lush and moist, the complexion will be rosy, and if the qi and blood yin and yang are unbalanced, it will manifest as facial disfiguring diseases such as spots, sores, and wrinkles. For example, if the qi and blood are insufficient, qi stagnation will easily lead to blood stasis and form spots; if the qi is excessive and the yin blood is insufficient, it will easily become depressed and turn into heat, which will rush up to the face and cause acne; spleen blood deficiency will lead to spleen dysfunction and wrinkles. Heat-clearing drugs are mostly related to anti-inflammatory effects in modern medical research. Heat-clearing and detoxifying drugs can act on the body's inflammatory chemokines to interfere with the inflammatory state, achieving the purpose of beauty care and treatment of disfiguring diseases such as acne. At the same time, in terms of meridians, the present invention is mainly based on the liver, spleen, and stomach meridians in terms of application frequency. The spleen is responsible for ascending the clear, is the source of qi and blood biochemistry, and is the basis of beauty care. If the spleen and stomach function normally, the food and water yang qi will be transformed into fine and fine, and the fine and fine will rise and disperse, nourishing the five internal organs, the body will be rich in essence and the eyes will be magical, the face will be ruddy, the muscles will be full and strong, etc., so the normal spleen and stomach function is the basis of beauty care. Therefore, the present invention adopts Chinese medicine for promoting blood circulation, nourishing blood, cooling blood, strengthening spleen and harmonizing stomach and donkey skin to be compatible, which can resist inflammation, remove internal fire evil, promote blood circulation and remove blood stasis, and promote new blood circulation to achieve the purpose of beautifying and nourishing the skin.

[0043] Example 1

[0044] A donkey-hide gelatin for beautifying and reducing spots comprises the following raw materials in parts by weight: 80 parts of donkey skin, 40 parts of red date powder, 20 parts of yam powder, 20 parts of rose pollen, 10 parts of olive oil, 5 parts of crystal sugar, 5 parts of angelica extract, 5 parts of coix seed powder and 8 parts of rice wine.

[0045] The preparation process is as follows:

[0046] 1) respectively taking red dates, Chinese yam, rose and coix seeds, washing them, freeze-drying them at -20°C, ultrafine grinding, sieving and sterilizing to obtain red date powder, Chinese yam powder, rose powder and coix seeds powder with a mesh size of 700-800, weighing them according to the amount, and setting aside;

[0047] Then, the angelica powder was dried and crushed, passed through a 40-mesh sieve, and the angelica powder was added to a 70% ethanol aqueous solution with a mass volume ratio of m:v=1kg:8L, and soaked for 20 minutes, and refluxed for 2 times, 1 hour each time;

[0048] 2) Soak the refined donkey hide in water for 5 days, with the amount of water added being preferably enough to cover the donkey hide; shave the hair, cut the donkey hide into pieces with a side length of about 30 cm, and wash the pieces in clean water until there is no impurity on the surface; then add sodium carbonate at 0.5% of the weight of the pieces, add water at a mass volume ratio of 1kg:0.5L of the pieces, and blanch the skin with steam for 30 minutes to obtain wet skin;

[0049] 3) the wet leather obtained in step 2) is mixed with water in a mass volume ratio of 1kg:0.5L and put into a steaming ball, and steam is dynamically extracted for 1h at 0.12Mpa, and static extraction is performed for 5h to obtain gum juice; then water is added in a mass volume ratio of 1kg:0.5L of wet leather to water, and steam is dynamically extracted for 1h at 0.12Mpa, and the process of adding water and steam is repeated until the wet leather is completely hydrolyzed and melted; to obtain gum juice;

[0050] 4) The gelatin obtained in step 3) is pumped into a concentrator and concentrated to a relative density of about 1.08, during which the foam and impurities on the surface of the gelatin are skimmed off; then the gelatin is pumped into a container and concentrated at 30°C and a vacuum degree of -0.06Mpa for 1 hour, and the foam is extracted. Water is added, concentrated, and foam extracted. The process of adding water, concentrating, and foaming is repeated until the gelatin is beaded, and red date powder, yam, rose pollen, olive oil, crystal sugar, angelica extract, coix seed powder, and rice wine are added, and the gelatin is further concentrated to a thick paste, first condensed at 2°C for 10 hours, and then condensed at 0°C for 1 hour; the gelatin is cut, and then aired at a temperature of 16°C and a humidity of 55% for 44 days until the moisture content is about 14%; the gelatin is cut, the gelatin is wiped, and ultraviolet radiation sterilization is performed at 30°C for 25 minutes, and the finished gelatin is packaged to obtain the finished product.

[0051] Example 2

[0052] A donkey-hide gelatin for beautifying and reducing spots comprises the following raw materials in parts by weight: 100 parts of donkey skin, 36 parts of red date powder, 12 parts of yam powder, 17 parts of rose pollen, 13 parts of olive oil, 10 parts of crystal sugar, 6 parts of angelica extract, 6 parts of coix seed powder and 5 parts of rice wine.

[0053] The preparation process is as follows:

[0054] 1) respectively taking red dates, Chinese yam, rose and coix seeds, washing them, freeze-drying them at -30°C, ultrafine grinding, sieving and sterilizing them to obtain red date powder, Chinese yam powder, rose powder and coix seeds powder with a mesh size of 700-800, weighing them according to the amount, and setting aside;

[0055] Then, the angelica powder was dried and crushed, passed through a 50-mesh sieve, and the angelica powder was added to an ethanol solution with a volume concentration of 80% according to a mass volume ratio of angelica powder to ethanol solution of m:v=1kg:10L, and soaked for 30 minutes, and refluxed for extraction 3 times, 1.5 hours per time;

[0056] 2) Soak the donkey hide after retrieval in water for 6 days, with the amount of water added being preferably enough to cover the hide; shave the hair, cut the hide into pieces with a side length of about 32 cm, and wash the pieces in clean water until there is no impurity on the surface; then add sodium carbonate at 0.5% of the weight of the pieces, add water at a mass volume ratio of 1kg:0.5L of the pieces, and blanch the hide in steam for 35 minutes to obtain wet hide;

[0057] 3) the wet skin obtained in step 2) is mixed with water in a mass volume ratio of 1kg:0.6L and put into a steaming ball, and steam dynamic extraction is performed at 0.13Mpa for 1.5 hours, and static extraction is performed for 5.5 hours to obtain juice; then water is added in a mass volume ratio of 1kg:0.6L of wet skin to water, and steam dynamic extraction is performed at 0.14Mpa for 1.5 hours, and the process of adding water and steam dynamic extraction is repeated until the wet skin is completely hydrolyzed and melted; to obtain gum juice;

[0058] 4) The gelatin obtained in step 3) is pumped into a concentrator and concentrated to a relative density of about 11.09, during which the foam and impurities on the surface of the gelatin are skimmed off; then the gelatin is pumped into a container and concentrated at 35°C and a vacuum degree of -0.07Mpa for 1.5 hours, the foam is extracted, water is added, concentrated, and the foam is extracted, and the process of adding water, concentrating, and extracting foam is repeated until the gelatin is beaded, and red date powder, yam, rose pollen, olive oil, crystal sugar, angelica extract, coix seed powder and rice wine are added, and the gelatin is further concentrated to a thick paste, first condensed at 4°C for 11 hours, and then condensed at 1°C for 1.5 hours; the gelatin is cut, and then aired at a temperature of 20°C and a humidity of 65% for 50 days until the moisture content is about 12%; the gelatin is cut, the gelatin is wiped, and ultraviolet radiation sterilization is performed at 35°C for 30 minutes, and the finished donkey-hide gelatin is obtained.

[0059] Example 3

[0060] A donkey-hide gelatin for beautifying and reducing spots comprises the following raw materials in parts by weight: 150 parts of donkey skin, 30 parts of red date powder, 10 parts of yam powder, 10 parts of rose pollen, 15 parts of olive oil, 15 parts of crystal sugar, 10 parts of angelica extract, 10 parts of coix seed powder and 3 parts of rice wine.

[0061] The preparation process is as follows:

[0062] 1) respectively taking red dates, Chinese yam, rose and coix seeds, washing them, freeze-drying them at -40°C, ultrafine grinding, sieving and sterilizing them to obtain red date powder, Chinese yam powder, rose powder and coix seeds powder with a mesh size of 700-800, weighing them according to the amount, and setting aside;

[0063] Then, the angelica powder was dried and crushed, passed through a 60-mesh sieve, and the angelica powder was added to a 90% ethanol aqueous solution with a mass volume ratio of m:v=1kg:12L, and soaked for 40 minutes, and refluxed for 4 times, 2 hours per time;

[0064] 2) Soak the refined donkey hide in water for 7 days, with the amount of water added being preferably enough to cover the donkey hide; shave the hair, cut the donkey hide into pieces with a side length of about 35 cm, and wash the pieces in clean water until there is no impurity on the surface; then add sodium carbonate at 0.5% of the weight of the pieces, add water at a mass volume ratio of 1kg:0.5L of the pieces, and steam blanch the hide for 40 minutes to obtain wet hide;

[0065] 3) the wet skin obtained in step 2) is mixed with water in a mass volume ratio of 1kg:0.7L and put into a steaming ball, and steam is dynamically extracted for 2h at 0.15Mpa, and static extraction is performed for 6h to obtain juice; then water is added in a mass volume ratio of 1kg:0.7L of wet skin to water, and steam is dynamically extracted for 2h at 0.15Mpa, and the process of adding water and steam is repeated until the wet skin is completely hydrolyzed and melted; to obtain gum juice;

[0066] 4) The gelatin obtained in step 3) is pumped into a concentrator and concentrated to a relative density of about 1.08, during which the foam and impurities on the surface of the gelatin are skimmed off; then the gelatin is pumped into a container and concentrated at -40°C and a vacuum degree of -0.08Mpa for 2 hours, and the foam is extracted. Water is added, concentrated, and foam extracted. The process of adding water, concentrating, and foam extraction is repeated until the gelatin is beaded, and red date powder, yam, rose pollen, olive oil, crystal sugar, angelica extract, coix seed powder, and rice wine are added, and the gelatin is further concentrated to a thick paste, first condensed at 7°C for 12 hours, and then condensed at 2°C for 2 hours; the gelatin is cut, and then aired at a temperature of 25°C and a humidity of 70% for 62 days until the moisture content is about 15%; the gelatin is cut, the gelatin is wiped, and ultraviolet radiation sterilization is performed at 40°C for 35 minutes, and the finished donkey-hide gelatin is obtained.

[0067] The donkey-hide gelatin prepared in Examples 1-3 was used as Group 1, Group 2 and Group 3 of the present invention to conduct subsequent experiments. Meanwhile, a comparative example was set up, and the grouping was as follows:

[0068] Comparative Example 1 (removing olive oil): 100 parts of donkey skin, 36 parts of red date powder, 12 parts of yam powder, 17 parts of rose pollen, 10 parts of crystal sugar, 19 parts of angelica extract, 6 parts of coix seed powder and 5 parts of rice wine.

[0069] Comparative Example 2 (excluding angelica extract): 100 parts of donkey skin, 36 parts of red date powder, 12 parts of yam powder, 17 parts of rose pollen, 19 parts of olive oil, 10 parts of crystal sugar, 6 parts of coix seed powder and 5 parts of rice wine.

[0070] Comparative Example 3 (removing the donkey skin): 36 parts of red date powder, 12 parts of yam powder, 17 parts of rose pollen, 63 parts of olive oil, 10 parts of crystal sugar, 56 parts of angelica extract, 6 parts of coix seed powder and 5 parts of rice wine.

[0071] Comparative Example 4 (olive oil replaced by soybean oil): 100 parts of donkey skin, 36 parts of red date powder, 12 parts of yam powder, 17 parts of rose pollen, 13 parts of soybean oil, 10 parts of crystal sugar, 6 parts of angelica extract, 6 parts of coix seed powder and 5 parts of rice wine.

[0072] Comparative Example 5 (olive oil is replaced by walnut oil): 100 parts of donkey skin, 36 parts of red date powder, 12 parts of yam powder, 17 parts of rose pollen, 13 parts of walnut oil, 10 parts of crystal sugar, 6 parts of angelica extract, 6 parts of coix seed powder and 5 parts of rice wine.

[0073] Comparative Example 7 (removing coix seed powder): 100 parts of donkey skin, 36 parts of red date powder, 12 parts of yam powder, 17 parts of rose pollen, 13 parts of olive oil, 10 parts of crystal sugar, 6 parts of angelica extract and 5 parts of rice wine.

[0074] Comparative Example 8 (removing rose pollen): 100 parts of donkey skin, 36 parts of red date powder, 12 parts of yam powder, 13 parts of walnut oil, 10 parts of crystal sugar, 6 parts of angelica extract, 6 parts of coix seed powder and 5 parts of rice wine.

[0075] Comparative Example 9 (removing yam powder): 100 parts of donkey skin, 36 parts of red date powder, 17 parts of rose pollen, 13 parts of walnut oil, 10 parts of crystal sugar, 6 parts of angelica extract, 6 parts of coix seed powder and 5 parts of rice wine.

[0076] Comparative Example 10 (excluding angelica extract and olive oil): 100 parts of donkey skin, 36 parts of red date powder, 12 parts of yam powder, 17 parts of rose pollen, 10 parts of crystal sugar, 6 parts of coix seed powder and 5 parts of rice wine.

[0077] Comparative Example 11 (donkey-hide gelatin on the market): 100 parts of donkey skin, 3.2 parts of crystal sugar, 3.2 parts of soybean oil, and 0.8 parts of rice wine.

[0078] The experimental process is as follows:

[0079] Experiment 1 Animal experiment verifies the anti-aging properties of the product of the present invention

[0080] Traditional Chinese medicine believes that human aging and facial pigmentation are related to whether the Qi and blood are abundant, whether the Qi and blood flow smoothly, whether the liver Qi is stagnant, and whether the spleen and stomach function normally. Therefore, this experiment was first conducted on rats to verify the anti-aging properties of the product of the present invention at the molecular level.

[0081] Experimental animals and equipment: 10-week-old SD rats;

[0082] MDA, SOD, and GSH-Px assay kits (purchased from Wuhan Gilead Biotechnology Co., Ltd.);

[0083] D-galactose was purchased from Xi'an Dafengshou Biotechnology Co., Ltd.

[0084] Sample dosage: The donkey-hide gelatin paste of the present invention is ultrafinely ground into 200 meshes, and the sample dosage for rats is 210 mg / kg BW.

[0085] Modeling, grouping and experiment: 160 SD rats aged 10 weeks, weighing 200±20 g, were randomly divided into 16 groups, 10 rats in each group, namely the model group, Example 1-3 groups, Comparative Example 1-11 groups and the normal group.

[0086] The model group, the example 1-3 group, and the comparative example 1-11 group were subcutaneously injected with D-galactose (dosage of 0.1 ml / 10 g) from the back of the neck at 1.3 g / kg every day, and the normal group was injected with an equal amount of physiological saline; 25-35 minutes after the injection of D-galactose, the products of the present invention group 1-3 and the comparative example 1-11 were fed once according to the sample dose. Feeding was done twice a day, and after the operation was completed, all groups of rats were free to eat and drink water, and the feeding was continued for 3 months. Blood was collected from the inferior vena cava, and the serum superoxide dismutase (SOD), serum malondialdehyde (MDA) content and glutathione peroxidase (GSH-Px) activity of each group of rats were measured and counted, and the results are shown in Table 1.

[0087] Table 1 SOD, MDA content and GSH-Px activity in rat serum

[0088]

[0089]

[0090] As shown in Table 1, when any one of the components olive oil, donkey skin or angelica extract is removed, the SOD enzyme and GSH-Px activities in the rat serum are greatly reduced compared with the normal group, and the MDA content is increased compared with the normal group, and there is a significant difference compared with the model group and the normal group, and there is also a significant difference compared with the embodiment of the present invention, which shows that the olive oil, donkey skin or angelica extract in the present invention synergizes and can greatly improve the anti-aging ability of the human body. At the same time, compared with Comparative Example 10, Comparative Examples 1-3, and the embodiment, the angelica extract and olive oil are removed from the donkey skin, and the antioxidant activity is greatly reduced, and the difference is significant, indicating that the addition of angelica extract and olive oil has achieved unexpected technical effects.

[0091] Experiment 2: Human Volunteer Experiment

[0092] In the early stage of the present invention, toxic and side effects were verified by rat experiments, and it was found that there was no obvious effect on the morphology and structure of the mouse spleen, and the level of anti-inflammatory factors in the serum was not significantly increased, indicating that the product of the present invention is safe and reliable.

[0093] 120 female volunteers aged 20-30 years were selected. Western medical equipment detected that there was no organic lesions in the liver, spleen, and stomach. Their faces were dark yellow, their skin was dull, they had many spots, and their facial skin was rough.

[0094] The 120 volunteers were randomly divided into 12 groups. After the agreement, they were informed of the donkey-hide gelatin formula of the present invention. After the volunteers agreed, they tried the donkey-hide gelatin paste of group 2 of the present invention and groups 1-11 of comparative examples for 3 months, eating 100g of donkey-hide gelatin paste every morning and evening. The skin conditions (gloss, roughness, and stain deposition) of the volunteers in each group were recorded after 1 month, 2 months, and 3 months. Among them, the skin gloss was measured by Ski n Gloss Meter, SGM2008, and the larger the value, the more glossy the skin; the skin roughness parameter was a parameter obtained by Primos CR taking a 3D image of the local cheek skin of the subject, and then analyzing it with professional analysis software, and was characterized by the Sa value. The Sa value is the arithmetic mean of the height of each point within the measurement area; the lower the value of the parameter, the smoother the skin.

[0095] Each group of volunteers was measured before taking the product and the average was taken; the values ​​were also measured after taking the product for 1 month (recorded as 1M), 2 months (recorded as 2M), and 3 months (recorded as 3M) and the average was taken;

[0096] Before the experiment, the average glossiness of the volunteers corresponding to Example 2 of the present invention was 51.04, Comparative Example 1 was 51.12, Comparative Example 2 was 50.74, Comparative Example 3 was 51.48, Comparative Example 4 was 50.26, Comparative Example 5 was 52.75, Comparative Example 6 was 51.31, Comparative Example 7 was 49.58, Comparative Example 8 was 49.42, Comparative Example 9 was 51.37, Comparative Example 10 was 50.08, and Comparative Example 11 was 49.63.

[0097] The roughness of the volunteers corresponding to Example 2 of the present invention is 25.26, the roughness of Comparative Example 1 is 25.19, the roughness of Comparative Example 2 is 25.02, the roughness of Comparative Example 3 is 25.17, the roughness of Comparative Example 4 is 25.86, the roughness of Comparative Example 5 is 25.05, the roughness of Comparative Example 6 is 25.41, the roughness of Comparative Example 7 is 25.37, the roughness of Comparative Example 8 is 25.09, the roughness of Comparative Example 9 is 25.04, the roughness of Comparative Example 10 is 25.13, and the roughness of Comparative Example 11 is 25.08.

[0098] After using Example 2 of the present invention and Comparative Examples 1-11, the data are shown in Table 2;

[0099] Table 2 Roughness, glossiness and color spots of each group

[0100]

[0101]

[0102] The changes in roughness (Sa, um) and glossiness (SGU) of each group after taking 1M, 2M, and 3M of donkey-hide gelatin were calculated using Table 2, as shown in Table 3.

[0103] Table 3 Changes in roughness and glossiness of each group after taking donkey-hide gelatin

[0104]

[0105] It can be seen from Tables 2 and 3 that when any one of the components of olive oil, donkey skin or angelica extract is removed, the skin glossiness of the volunteers is significantly lower than that of the present invention group, and the roughness is significantly greater than that of the present invention group; this shows that the donkey skin, olive oil and angelica extract in the present invention work synergistically, greatly increasing the beauty and skin care efficacy of donkey-hide gelatin paste, promoting the circulation of qi and blood, regulating the spleen and stomach, strengthening the spleen and removing dampness, soothing the liver and relieving depression, thereby making the face radiant.

[0106] Experiment 3: Human Volunteer Experiment

[0107] The volunteers were also selected from Experiment 2, and the method of taking the medicine was the same as that in Experiment 2. The sensory evaluation of each volunteer after taking the donkey-hide gelatin paste was counted, see Table 4.

[0108] Table 4 Sensory evaluation of volunteers after taking donkey-hide gelatin paste

[0109]

[0110]

[0111] The various embodiments in this specification are described in a progressive manner, and each embodiment focuses on the differences from other embodiments. The same or similar parts between the various embodiments can be referenced to each other.

[0112] The above description of the disclosed embodiments enables one skilled in the art to implement or use the present invention. Various modifications to these embodiments will be apparent to one skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to the embodiments shown herein, but rather to the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A donkey-hide gelatin for beautifying and reducing spots, characterized in that: The donkey-hide gelatin comprises the following raw materials in parts by weight: 80-150 parts of donkey skin, 30-40 parts of red date powder, 10-20 parts of yam powder, 10-20 parts of rose pollen, 10-15 parts of olive oil, 5-15 parts of crystal sugar, 5-10 parts of angelica extract, 5-10 parts of coix seed powder and 3-8 parts of rice wine.

2. The donkey-hide gelatin for beautifying and reducing pigmentation according to claim 1, characterized in that: The donkey-hide gelatin comprises the following raw materials in optimal weight proportions: 100 parts of donkey skin, 36 parts of red date powder, 12 parts of yam powder, 17 parts of rose pollen, 13 parts of olive oil, 10 parts of crystal sugar, 6 parts of angelica extract, 6 parts of coix seed powder and 5 parts of rice wine.

3. The method for preparing donkey-hide gelatin according to any one of claims 1 to 2, characterized in that: The process is as follows: 1) preparing red date powder, yam powder, rose pollen, angelica extract, and coix seed powder respectively, and weighing them according to the amounts, and setting aside; 2) Pre-treat the donkey hide after picking and refining to obtain wet hide; 3) the wet skin obtained in step 2) is mixed with water in a mass volume ratio of 1kg:0.5-0.7L and put into a steaming ball, and steam is dynamically extracted for 1-2h at 0.12-0.15mpa, and static extraction is performed for 5-6h to obtain juice; then water is added in a mass volume ratio of 1kg:0.5-0.7L of wet skin to water, and steam is dynamically extracted for 1-2h at 0.12-0.15mpa, and the process of adding water and steam is repeated until the wet skin is completely hydrolyzed and melted; to obtain gum juice; 4) The gelatin obtained in step 3) is initially concentrated, foamed, concentrated again, foamed, water is added, concentrated, and foamed, and the process of adding water, concentrating, and foaming is repeated until the gelatin is beaded, and red date powder, yam, rose pollen, olive oil, crystal sugar, angelica extract, coix seed powder and rice wine are added, and the gelatin is further concentrated to a thick paste, gelled, cut, dried, cut, wiped, sterilized, and packaged to obtain the finished donkey-hide gelatin.

4. The preparation method according to claim 3, characterized in that: In step 1), the preparation process of red date powder, yam powder, rose pollen and coix seed powder is as follows: Red dates, yam, rose and coix seeds are taken respectively, washed, freeze-dried at -20 to -40°C, ultrafinely crushed, sieved and sterilized to obtain red date powder, yam powder, rose pollen and coix seeds powder with a mesh size of 700-800.

5. The preparation method according to claim 3, characterized in that: The preparation process of the angelica extract in step 1) is as follows: the angelica is dried and crushed, passed through a 40-60 mesh sieve, and the angelica powder is added to the ethanol aqueous solution and soaked according to the mass volume ratio of the angelica powder to the ethanol aqueous solution of m:v=1kg:8-12L, refluxed for 2-4 times, and the extracts are combined; Wherein, the volume concentration of ethanol in the ethanol aqueous solution is 70%-90%; the soaking time is 20-40 minutes; and the extraction time is 1-2 hours / time.

6. The preparation method according to claim 3, characterized in that: In step 2), the pretreatment is to soak, shave, cut, wash and blanch the donkey skin after picking and refining in sequence; Wherein, the soaking is performed by adding water for 5 to 7 days; the amount of water added is preferably such that the surface of the water covers the donkey skin; The skinning is to cut the donkey skin into pieces with a side length of 30-35 cm; Washing the leather is to place the leather in clean water and wash it until there is no impurity on the surface; The blanching process comprises adding sodium carbonate at 0.5% of the weight of the leather block, then adding water at a mass volume ratio of the leather block to water of 1kg:0.5L, and blanching the leather with steam for 30-40 minutes.

7. The preparation method according to claim 3, characterized in that: In step 4), the preliminary concentration is to pump the gel juice into a concentrator and concentrate it to a relative density of 1.08-1.09, and then pump it into a container for further concentration; The foaming step is to skim off the foam and impurities on the surface of the glue; The re-concentration is carried out at 30-40° C. and a vacuum degree of -0.06 to -0.08 MPa for 1-2 h.

8. The preparation method according to claim 3, characterized in that: In step 4), the gel is first condensed at 2-7°C for 10-12h, and then condensed at 0-2°C for 1-2h; The drying of the glue is carried out at a temperature of 16-25° C. and a humidity of 55%-70% for 44-62 days until the moisture content is ≤15wt%; The sterilization is carried out at 30-40°C and under ultraviolet radiation for 25-35 minutes.

9. The use of donkey-hide gelatin according to any one of claims 1-2, characterized in that: The use is for preparing external medicines or skin care products with beauty or skin care effects.

10. The use according to claim 9, characterized in that The external medicines include but are not limited to acne creams; the skin care products include but are not limited to facial masks.

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