Application of yeast beta-glucan in improving intestinal function

Abstract

The invention relates to the technical field of yeast glucan, in particular to yeast beta-glucan which can promote proliferation of gram-positive beneficial active bacteria in intestinal tracts, inhibit proliferation of gram-negative saprophytic bacteria such as escherichia coli, salmonella and other pathogenic bacteria in the intestinal tracts, contribute to reduction of the number of harmful bacteria in the intestinal tracts of human bodies, and improve the immunity of the intestinal tracts. The proportion of beneficial bacteria is increased, so that the intestinal flora structure is optimized, and the intestinal environment is improved. The yeast beta-glucan promotes proliferation of beneficial bacteria, inhibits pathogenic bacteria, optimizes intestinal flora and enhances the immune function. The intestinal health is protected by improving I gA secretion. Beta-glucan extracted by neutralizing pectinase contains monosaccharide molecules, can be absorbed and utilized, promotes growth of beneficial bacteria, inhibits harmful bacteria, maintains intestinal microecological balance, contributes to nutrient absorption and reduces intestinal diseases.

Description

Technical Field

[0001] The present invention relates to the technical field of yeast beta-glucan, and in particular to application of yeast beta-glucan in improving intestinal function. Background Art

[0002] Yeast glucan, also known as glucose anhydride, is a polysaccharide that exists in the mucus secreted by some microorganisms during their growth. Glucan has a high molecular weight and is mainly composed of D-glucose pyranose connected by α, 1→6 bonds. The branch points are connected by 1→2, 1→3, and 1→4. The β-glucan bound by β-1,3 / -1,6 has the highest biological activity. Its structure and function are different depending on the type of microorganism and growth conditions. A Chinese patent discloses a yeast glucan production process (Announcement No. CN112126665A). This patented technology solves the problem that the traditional yeast glucan preparation method is complicated, costly, and not suitable for large-scale production, especially the preparation conditions are relatively harsh, the equipment environment requirements are harsh, and the equipment used is expensive, resulting in high yeast glucan preparation costs. Therefore, a yeast glucan production process is proposed.

[0003] However, the prior art does not mention the application of yeast β-glucan in improving intestinal function, and it is necessary to solve the problem that the yeast β-glucan in the prior art may contain single molecule sugars to increase intestinal improvement when extracted.

[0004] Therefore, those skilled in the art provide the use of yeast β-glucan in improving intestinal function to solve the problems raised in the above background technology. Summary of the invention

[0005] In order to solve the above technical problems, the present invention provides:

[0006] The present invention provides an application of yeast β-glucan, that is, an application of yeast β-glucan in improving intestinal function;

[0007] The yeast β-glucan of the present invention can promote the proliferation of Gram-positive beneficial active bacteria in the intestine, inhibit the proliferation of Gram-negative saprophytic bacteria in the intestine, such as pathogenic bacteria such as Escherichia coli and Salmonella, and help reduce the number of harmful bacteria in the human intestine and increase the proportion of beneficial bacteria, thereby optimizing the intestinal flora structure and improving the intestinal environment;

[0008] After entering the human intestine, β-glucan can increase the secretion of secretory IgA on the intestinal surface and the secretion of immunoglobulins in the intestine, thereby enhancing the immune barrier function of the intestinal mucosa. After entering the intestine, yeast β-glucan increases the secretion of IgA. Yeast β-glucan helps protect the intestine from the invasion of pathogens and maintain intestinal health.

[0009] Yeast β-glucan extracted by neutralization with pectinase will retain more monosaccharide molecules in the yeast β-glucan. The monosaccharide molecules in the yeast β-glucan can be directly absorbed by the epithelial cells of the small intestine and enter the blood circulation for use by various parts of the body. At the same time, the yeast β-glucan that neutralizes pectinase can also promote the growth of beneficial bacteria in the intestine and inhibit the reproduction of harmful bacteria, thereby maintaining the balance of intestinal microecology, facilitating the absorption and utilization of nutrients, and reducing the occurrence of intestinal diseases.

[0010] Yeast β-glucan promotes the proliferation of beneficial bacteria, inhibits pathogens, optimizes intestinal flora, and enhances immune function. It protects intestinal health by increasing IgA secretion. β-glucan extracted by neutralizing pectinase contains monosaccharide molecules that can be absorbed and utilized, promotes the growth of beneficial bacteria, inhibits harmful bacteria, maintains the balance of intestinal microecology, helps nutrient absorption, and reduces intestinal diseases. DETAILED DESCRIPTION

[0011] The specific embodiments of the present invention are further described in detail. The examples of the present invention are given for the purpose of illustration and description, and are not intended to be exhaustive or to limit the present invention to the disclosed forms. Many modifications and variations will be apparent to those of ordinary skill in the art. The embodiments are selected and described in order to better illustrate the principles and practical applications of the present invention, and to enable those of ordinary skill in the art to understand the present invention and design various embodiments with various modifications suitable for specific uses.

[0012] Example 1: In this example, yeast β-glucan is used to improve intestinal function. Yeast β-glucan can promote the proliferation of Gram-positive beneficial active bacteria in the intestine, inhibit the proliferation of Gram-negative saprophytic bacteria such as Escherichia coli, Salmonella and other pathogenic bacteria in the intestine, and help reduce the number of harmful bacteria in the human intestine and increase the proportion of beneficial bacteria, thereby optimizing the intestinal flora structure and improving the intestinal environment.

[0013] After entering the human intestine, β-glucan can increase the secretion of secretory IgA on the intestinal surface and the secretion of immunoglobulins in the intestine, thereby enhancing the immune barrier function of the intestinal mucosa. After entering the intestine, yeast β-glucan increases the secretion of IgA. Yeast β-glucan helps protect the intestine from the invasion of pathogens and maintain intestinal health.

[0014] Yeast β-glucan extracted by neutralization with pectinase will retain more monosaccharide molecules in the yeast β-glucan. The monosaccharide molecules in the yeast β-glucan can be directly absorbed by the small intestinal epithelial cells and enter the blood circulation for use by various parts of the body. At the same time, the yeast β-glucan that neutralizes pectinase can also promote the growth of beneficial bacteria in the intestine and inhibit the reproduction of harmful bacteria, thereby maintaining the balance of intestinal microecology, contributing to the absorption and utilization of nutrients, and reducing the occurrence of intestinal diseases.

[0015] Example 2: The optimization of intestinal flora by enzymatic hydrolysis of yeast β-glucan is carried out according to the following steps:

[0016] Step 1: Mix the yeast and pectinase solution. When the pectinase is mixed with stirring, deionized water is added to the pectinase to dissolve it, and then the pectinase and the yeast are placed in a stirrer and continuously mixed and stirred for 5 minutes to prepare a mixed solution;

[0017] Step 2: The mixed solution in step 1 is placed in an environment with a temperature range of 25 to 35° C. to promote pectinase to degrade the cell wall, and the enzyme decomposition time is 1 to 2 hours, wherein the solubility of the pectinase is 1% to 2% W / V, and the pH value under the temperature environment is 4 to 5.5;

[0018] Step 3: After the mixed solution in step 2 is hydrolyzed, it is placed in a centrifuge, the speed of the centrifuge for the mixed solution is 6000 rpm, and the stirring time is 5 to 10 minutes, and the degraded cell wall fragments are separated from the solution containing β-glucan by centrifugation;

[0019] Step 4: After centrifugation in step 3, the solution containing β-glucan is washed with water to remove residual enzymes. The ratio of β-glucan to water during the water washing is 1:10;

[0020] Step 5, placing the water in the β-glucan solution washed in step 3 into a spray dryer to evaporate the water in the β-glucan solution to obtain yeast β-glucan containing the enzyme for improving intestinal function;

[0021] The yeast β-glucan prepared as described above has a relatively high molecular weight, is mainly composed of D-glucose pyranose connected by α, 1→6 bonds, has branching points connected by 1→2, 1→3, and 1→4, and the β-glucan connected by β-1,3 / -1,6 has more biological activity;

[0022] The active structure of β-glucan is a polysaccharide composed of glucose units, which are bonded by β-1,3 to form yeast β-glucan;

[0023] Example 3. In this example, the use of yeast β-glucan in improving intestinal function is provided. Specifically, yeast β-glucan improves the intestinal tract.

[0024] Yeast β-glucan can promote the synthesis of intestinal healthy substances. Yeast β-glucan can stimulate intestinal cells to produce some beneficial substances, such as short-chain fatty acids, which are essential for maintaining intestinal health.

[0025] Among them, short-chain fatty acids can regulate the pH value of the intestine, inhibit the growth of harmful bacteria, promote the proliferation and repair of intestinal epithelial cells, and improve the digestion and absorption capacity of the intestine. By improving the structure of intestinal flora and enhancing the intestinal barrier function, yeast β-glucan can also improve the digestion and absorption capacity of nutrients in the intestine, which helps the body to better absorb and utilize the nutrients in food, thereby improving overall health.

[0026] Yeast β-glucan also has an antioxidant effect. Yeast β-glucan has a strong antioxidant capacity in the intestines, which can remove free radicals in the body and prevent free radicals from damaging intestinal cells. This antioxidant effect helps protect intestinal cells from oxidative stress and maintain the normal function and structure of intestinal cells.

[0027] Yeast β-glucan improves intestinal function through multiple mechanisms such as regulating the balance of intestinal flora, enhancing intestinal barrier function, promoting the synthesis of beneficial substances, improving intestinal digestion and absorption capacity, and anti-oxidation. These effects work together on the intestinal system, helping to maintain intestinal health and prevent the occurrence of intestinal diseases.

[0028] Example 4: In this example, yeast β-glucan is used to improve intestinal function and enhance intestinal function.

[0029] Yeast β-glucan, as a water-soluble dietary fiber, can increase stool volume and soften stool, thereby improving constipation symptoms. In addition, combined with the use of probiotics, it can significantly enhance the frequency of intestinal movement, significantly improve the number of bowel movements and stool viscosity, and relieve the symptoms of pain and difficulty in defecation;

[0030] Yeast β-glucan also has a positive effect on regulating intestinal metabolism. In a study on metabolic syndrome caused by a high-fat diet, yeast β-glucan was able to significantly adjust body weight, fat accumulation, and liver fatty degeneration, while reducing the levels of adverse metabolites in plasma.

[0031] The daily edible dosage of yeast beta-glucan in the present invention is between 200 and 400 mg.

[0032] Obviously, the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field and related fields without creative work should fall within the scope of protection of the present invention. The structures, devices and operating methods not specifically described and explained in the present invention are implemented according to the conventional means in the field unless otherwise specified and limited.

Claims

1. The application of yeast β-glucan in improving intestinal function is characterized by The application of yeast β-glucan in improving intestinal function; The application of yeast β-glucan enzymatic hydrolysis reaction to optimize intestinal flora is carried out according to the following steps: Step 1, mixing yeast and pectinase solution to prepare a mixed solution; Step 2: The mixed solution in step 1 is placed in an environment with a temperature range of 25 to 35° C. to promote pectinase to degrade the cell wall, and the enzyme decomposition time is 1 to 2 hours, wherein the solubility of the pectinase is 1% to 2% W / V; Step 3: After the mixed solution in step 2 is hydrolyzed, the mixed solution is placed in a centrifuge to separate the degraded cell wall fragments from the solution containing β-glucan by centrifugation; Step 4: After centrifugation in step 3, the solution containing β-glucan is washed with water to remove residual enzymes; Step 5: Place the water in the β-glucan solution washed in step 3 into a spray dryer to evaporate the water in the β-glucan solution to obtain yeast β-glucan containing the enzyme that improves intestinal function.