Application of sodium butyrate in improving yield of royal jelly

Abstract

The invention discloses application of sodium butyrate in improving the yield of royal jelly, and belongs to the technical field of biology and agriculture. In the royal jelly production process, a production group is fed with sodium butyrate with a certain concentration to promote intestinal structure development and intestinal barrier repair of the production group, meanwhile, the immunity of the organism is improved, the feed intake of worker bees is increased, and the feed digestibility is improved, so that the royal jelly yield is improved, and the royal jelly yield of a single queen cell can reach 105mg 48 hours after bees are transplanted. The method provided by the invention improves the yield of the royal jelly of the apis cerana, and can also be popularized to western bees and other bee species.

Description

Technical Field

[0001] The invention belongs to the field of biology and agricultural technology, and particularly relates to application of sodium butyrate in improving the yield of royal jelly. Background Art

[0002] Royal jelly, also known as royal jelly and bee milk, is the secretion of the hypopharyngeal gland and maxillary gland of young worker bees that cultivate larvae in the beehive. It is the food for worker bees and drone larvae, and the lifelong food of the queen bee. It is rich in nutrients and is known as "liquid gold". It is one of the main bee products in bee breeding and production. It has a good health-care effect and is widely loved by consumers. With the improvement of people's health awareness, the demand for royal jelly is increasing. The low production of royal jelly in beekeeping production is mainly due to the following reasons: 1. The production process is complicated and energy-intensive: bees obtain nutrients by eating pollen and honey, and secrete and produce royal jelly. This process requires a lot of time and energy, so bees can only produce limited royal jelly; 2. Worker bees have heavy tasks: worker bees are mainly responsible for collecting pollen and honey, and maintaining the normal operation of the hive. Among the worker bees, only nurse bees can secrete royal jelly. The limited number of nurse bees limits the production of royal jelly; 3. Seasonal influence: The production of royal jelly is affected by the season. In spring and summer, bees are most active, and the royal jelly production is the highest at this time. In autumn and winter, bees are less active, and the production of royal jelly will also decrease or stop production accordingly; 4. Health of the bee colony: The production of royal jelly is also affected by the health of the bee colony. If the bee colony is attacked by diseases or parasites, the production capacity of the bees will decrease, resulting in a decrease in the production of royal jelly; 5. Large demand: In a natural state, only the queen bee and larvae need royal jelly. In the case of artificial breeding, people also collect royal jelly from the hive. This leads to the need for bees to produce more royal jelly to meet demand. However, due to the various limitations mentioned above, bees are often unable to meet this demand, so the production of royal jelly always remains at a low level. The above reasons lead to low production of royal jelly and high labor costs, which restrict the production of royal jelly, especially the extremely low production of royal jelly of Chinese honey bees. At present, the market is dominated by royal jelly of Western honey bees.

[0003] In the research related to royal jelly production, significant progress has been made in increasing royal jelly of western honey bees through breeding and improving production technology, but there is less research on royal jelly production of oriental honey bees. Therefore, it is imperative to develop other methods to increase the royal jelly production of Chinese honey bees, enrich Chinese honey bee products, and help increase the benefits of Chinese honey bee breeding. Summary of the invention

[0004] In order to solve the above technical problems, the present invention proposes the application of sodium butyrate in increasing the production of royal jelly.

[0005] To achieve the above object, the present invention provides the following technical solutions:

[0006] The invention provides application of sodium butyrate in improving the yield of royal jelly.

[0007] Preferably, the application specifically comprises the following steps: mixing sodium butyrate and sucrose solution to obtain a sodium butyrate-sucrose solution; and feeding the bee colony with the obtained sodium butyrate-sucrose solution.

[0008] Preferably, the concentration of sodium butyrate in the sodium butyrate-sucrose solution is 10-80 mM / L.

[0009] Preferably, the concentration of sodium butyrate in the sodium butyrate-sucrose solution is 40 mM / L.

[0010] Preferably, the feeding amount is 300-500 mL per group per day.

[0011] Preferably, feeding starts 7 to 10 days before worm transfer.

[0012] Preferably, the feeding is terminated 2 to 3 days after the worms are transferred.

[0013] Preferably, the bee colony is a production colony.

[0014] Preferably, the preparation method of the sodium butyrate-sucrose solution is specifically as follows: firstly dissolving sodium butyrate with ultrapure water, and then adding sucrose syrup to make up the volume to obtain the sodium butyrate-sucrose solution.

[0015] Compared with the prior art, the present invention has the following advantages and technical effects:

[0016] The invention improves the royal jelly yield by feeding a production group with sodium butyrate of a certain concentration during the royal jelly production process.

[0017] The method provided by the invention improves the yield of royal jelly of Chinese honey bees, and can also be extended to western honey bees and other bee species. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] The drawings constituting a part of the present application are used to provide a further understanding of the present application. The illustrative embodiments and descriptions of the present application are used to explain the present application and do not constitute an improper limitation on the present application. In the drawings:

[0019] Figure 1 This is the effect of sodium butyrate concentration on royal jelly production in the present invention. DETAILED DESCRIPTION

[0020] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0021] In order to make the above-mentioned objects, features and advantages of the present invention more obvious and easy to understand, the present invention is further described in detail below with reference to the accompanying drawings and specific embodiments.

[0022] The embodiment of the present invention provides the application of sodium butyrate in increasing the production of royal jelly. The present invention promotes the development of the intestinal structure and repair of the intestinal barrier of the production group during the royal jelly production process, increases the feed intake of worker bees, and improves the digestibility of feed, thereby increasing the production of royal jelly.

[0023] In a preferred embodiment, the application specifically comprises the following steps: mixing sodium butyrate and sucrose solution to obtain sodium butyrate-sucrose solution; and feeding bee colonies with the obtained sodium butyrate-sucrose solution. The present invention first mixes sodium butyrate and sucrose solution to prepare a sodium butyrate-sucrose solution of a certain concentration, and then feeds the bee colonies with the obtained sodium butyrate-sucrose solution, which can increase the production of royal jelly.

[0024] In a preferred embodiment, the concentration of sodium butyrate in the sodium butyrate-sucrose solution is 10-80 mM / L, more preferably 40 mM / L.

[0025] In a preferred embodiment, the feeding amount is 300-500 mL per colony per day, the feeding time is evening, and the number of bees in each colony is about 16,000. The feeding amount in the present invention is determined according to the feeding method in daily management. A small feeding amount has a poor stimulation effect on the bee colony, and a large feeding amount may cause the bee colony to be unable to fully feed at night, resulting in adverse effects such as bee theft.

[0026] In a preferred embodiment, the timing of starting feeding is 7 to 10 days before worm transfer.

[0027] In a preferred embodiment, the feeding is terminated 2 to 3 days after the worms are transferred.

[0028] In a preferred embodiment, the bee colony is a production colony; the colony strength of the bee colony is 4 combs; and the bee species of the bee colony is Apis cerana.

[0029] In a preferred embodiment, the preparation method of the sodium butyrate-sucrose solution is specifically as follows: sodium butyrate is first dissolved in ultrapure water, and then sucrose syrup is added to make up the volume to obtain the sodium butyrate-sucrose solution; the mass ratio of sucrose to water in the sucrose syrup is 1:1. The sodium butyrate-sucrose solution in the present invention is prepared and used before each use.

[0030] In the following examples, the Chinese honey bees were raised in the experimental bee farm of the Guizhou Modern Agricultural Development Institute;

[0031] Sucrose was purchased from Guangxi Nanning Mingyang Sugar Co., Ltd.; sodium butyrate powder (NaB) was purchased from Beijing Solebow Technology Co., Ltd., Cat#S9491, CAS: 156-54-7, molecular weight 110.09 g / mol.

[0032] Examples 1-4 and Comparative Example 1

[0033] (1) Preparation of bee colonies: 15 bee colonies with 4 combs and no pests and diseases were selected as production colonies, and randomly divided into 5 groups, labeled as CK, 10NaB, 20NaB, 40NaB, and 80NaB, with 3 colonies in each group and 4 combs per colony with about 16,000 bees. In addition, 1 bee colony with uniform color, strong colony strength, and no pests and diseases was selected to provide small larvae.

[0034] (2) Preparation of sodium butyrate-sucrose solution: sucrose and water were dissolved in a mass ratio of 1:1 and boiled and cooled before use to obtain sucrose syrup; 0 g, 1.1 g, 2.2 g, 4.4 g, and 8.8 g of sodium butyrate powder were weighed and put into mineral water bottles marked as CK, 10 NaB, 20 NaB, 40 NaB, and 80 NaB, respectively, 20 mL of ultrapure water was added to each mineral water bottle to fully dissolve the sodium butyrate powder, and then sucrose syrup was added to make the volume to 1000 mL, and the mixture was fully mixed to obtain sodium butyrate-sucrose solutions with sodium butyrate concentrations of 0 mM / L (CK group, comparative example 1), 10 mM / L (10 NaB group, example 1), 20 mM / L (20 NaB group, example 2), 40 mM / L (40 NaB group, example 3), and 80 mM / L (80 NaB group, example 4).

[0035] (3) Feeding the production group with sodium butyrate-sucrose solution: Starting from 7 days before the worm transfer, the 5 production groups in step (1) were fed with sodium butyrate-sucrose solution of corresponding concentration every evening, 333 mL per group per day, and the feeding was stopped 2 days after the worm transfer (when royal jelly was taken).

[0036] (4) Transferring larvae to obtain royal jelly: 5 days before transferring larvae, select an empty comb and place it in a queen limiter. Place the bee colony with small larvae provided in step (1) in the queen limiter for 4 to 8 hours. In the evening of the same day, move the queen bee to the queen limiter and allow the queen bee to lay eggs on the designated comb for 12 hours before removing the queen bee. After 96 hours, transfer larvae to produce royal jelly. The transferred larvae are 1 to 1.5 days old. 30 larvae are transferred from each experimental colony.

[0037] 48 hours after the transfer, remove the royal jelly frame, pick out the larvae with tweezers, take out the royal jelly with a jelly pen, and weigh the royal jelly. Figure 1 .

[0038] Effect of sodium butyrate concentration on royal jelly production Figure 1 , a, b, c in the figure represent the significance level. Figure 1 It can be seen that feeding the production group with sodium butyrate-sucrose solution with sodium butyrate concentrations of 10mM / L, 20mM / L, 40mM / L, and 80mM / L can increase royal jelly production. The royal jelly production of the experimental group with a sodium butyrate concentration of 40mM / L increased significantly, with the best effect. The royal jelly production of a single queen cell can reach 105mg.

[0039] In summary, during the royal jelly production process, feeding a certain concentration of sodium butyrate to the production colony can increase the royal jelly production, and the royal jelly production of the experimental group with a sodium butyrate concentration of 40mM / L increased significantly. This method of adding sodium butyrate to the bee colony feed to increase the royal jelly production of Chinese honey bees may be extended to western honey bees and other bee species.

[0040] The above are only preferred specific implementations of the present application, but the protection scope of the present application is not limited thereto. Any changes or substitutions that can be easily thought of by a person skilled in the art within the technical scope disclosed in the present application should be included in the protection scope of the present application. Therefore, the protection scope of the present application should be based on the protection scope of the claims.

Claims

1. Application of sodium butyrate in increasing royal jelly production.

2. The use of sodium butyrate in increasing royal jelly production according to claim 1, characterized in that: The application specifically comprises the following steps: mixing sodium butyrate and sucrose solution to obtain a sodium butyrate-sucrose solution; and feeding a bee colony with the obtained sodium butyrate-sucrose solution.

3. The use of sodium butyrate in increasing royal jelly production according to claim 2, characterized in that: The concentration of sodium butyrate in the sodium butyrate-sucrose solution is 10-80 mM / L.

4. The use of sodium butyrate in increasing royal jelly production according to claim 3, characterized in that: The concentration of sodium butyrate in the sodium butyrate-sucrose solution is 40 mM / L.

5. The use of sodium butyrate in increasing royal jelly production according to claim 2, characterized in that: The feeding amount is 300 to 500 mL per group per day.

6. The use of sodium butyrate in increasing royal jelly production according to claim 2, characterized in that: The best time to start feeding is 7 to 10 days before worm transfer.

7. The use of sodium butyrate in increasing royal jelly production according to claim 2, characterized in that: The best time to end feeding is 2 to 3 days after the worms are transferred.

8. The use of sodium butyrate in increasing royal jelly production according to claim 2, characterized in that: The bee colony is a production colony.

9. The use of sodium butyrate in increasing royal jelly production according to claim 2, characterized in that: The preparation method of the sodium butyrate-sucrose solution is specifically as follows: firstly, sodium butyrate is dissolved in ultrapure water, and then sucrose syrup is added to make up the volume to obtain the sodium butyrate-sucrose solution.

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