Feed additive containing probiotics and preparation method thereof

Abstract

The invention discloses a feed additive containing probiotics and a preparation method thereof, and belongs to the technical field of biological feed. The preparation method comprises the following steps: (1) preparing a coating nuclear membrane: heating and melting polylactic acid, gelatin, sodium alginate, xanthan gum, glyceride and citric acid to obtain coating liquid; (2) preparing a coated core: transferring soybean protein, soybean curb residue, rice bran, dry yeast and probiotics into a feed liquid tank, fully stirring and uniformly mixing, and granulating to obtain granulated feed; (3) drying the pellet feed obtained in the step (2) in a fluidized bed; and (4) atomizing the coating liquid obtained in the step (1) on the surface of the pellet feed dried in the step (3) to obtain the pellet feed. The related preparation method has remarkable technical effects in the aspects of increasing the survival rate of probiotics, improving the nutritional value of the feed, promoting the growth and immunity of animals, improving the stability and economic benefits of the feed and the like, and meets the requirements of the modern breeding industry.

Description

Technical Field

[0001] The present invention belongs to the field of biological feed technology, and specifically relates to a feed additive containing probiotics and a preparation method thereof. With the rapid development of modern aquaculture, the use of feed nutrients and additives has gradually received attention, especially the application of probiotics, which has become an important means to improve animal growth performance and improve intestinal health. Background Art

[0002] Probiotics refer to a class of live microorganisms that can have beneficial effects on the host. They improve the balance of intestinal microbiota, enhance the body's immune function, and promote the absorption of nutrients, thereby increasing the growth rate and feed conversion rate of animals. The application of probiotics is not limited to animal feed, but also has a wide range of applications in human food, health products and other fields.

[0003] In animal husbandry, the role of probiotics is mainly reflected in the following aspects:

[0004] Promote growth: Probiotics can improve the intestinal environment by inhibiting the growth of harmful bacteria, thereby increasing the growth rate and feed utilization of animals.

[0005] Enhance immunity: Probiotics stimulate the activity of immune cells in the intestine, enhance the body's nonspecific immune function and reduce the incidence of disease.

[0006] Improve intestinal health: Probiotics can promote the proliferation of beneficial bacteria, inhibit the growth of pathogenic bacteria, maintain the balance of intestinal microecology, and reduce digestive problems such as diarrhea.

[0007] Improve feed conversion rate: Probiotics can improve feed conversion rate by breaking down the complex components in feed and improving the digestion and absorption efficiency of nutrients.

[0008] Current status of probiotic feed additives

[0009] With the increasing emphasis on animal health and growth performance, the market demand for probiotic feed additives has gradually increased. In the current market, the main ingredients of probiotic feed additives include lactic acid bacteria, bifidobacteria, yeast, etc. The application of these probiotics has achieved remarkable results in the feed of many animals such as pigs, chickens, and cattle.

[0010] However, existing probiotic feed additives have some technical problems in the production, transportation and storage process. For example, probiotics are easily affected by environmental factors such as temperature and humidity during storage, which leads to a decrease in the number of live bacteria and affects their effect in feed. In addition, many existing products are mainly filled with wheat bran, corn flour, etc. The properties of these fillers will affect the adhesion properties of probiotics, further reducing their stability and effectiveness.

[0011] The preparation of probiotics usually includes the following steps:

[0012] Selection and cultivation of bacterial strains: Select suitable probiotic strains and cultivate them in appropriate culture media to obtain high concentrations of live bacteria.

[0013] Protection of live bacteria: In order to improve the survival rate of probiotics, they usually need to be protected, such as freeze drying, spray drying, etc. These methods can effectively extend the shelf life of probiotics.

[0014] Formula optimization: When preparing probiotic feed additives, it is necessary to optimize the formula according to the physiological characteristics and nutritional needs of different animals to achieve the best feed effect.

[0015] Evaluation of application effects: In actual applications, it is necessary to evaluate the effects of probiotic feed additives, including their effects on animal growth performance, feed conversion rate, intestinal health, etc.

[0016] Although the use of probiotics in feed has significant advantages, it still faces some challenges:

[0017] Selection of bacterial strains: Different probiotic strains have different effects in different animals, so how to choose the right strain is an important issue.

[0018] Stability and survival rate: The survival rate of probiotics is affected by many factors, including production process, storage conditions, etc. Improving the survival rate of probiotics is the key to improving their effectiveness.

[0019] Economical: The production cost of probiotic feed additives is relatively high. How to reduce costs and improve economic benefits is an important factor in promoting their application.

[0020] Regulations and standards: As feed additives, the safety and effectiveness of probiotics need to comply with relevant regulations and standards, which puts higher requirements on their market promotion and application.

[0021] In order to meet the above challenges, current research directions mainly focus on the following aspects:

[0022] Development of novel probiotics: Screening and cultivating novel probiotics to explore their potential for application in animal feed.

[0023] Microencapsulation technology: Microencapsulation technology is used to protect probiotics and improve their stability and survival rate in feed.

[0024] Research on compound additives: combining probiotics with other functional ingredients (such as enzymes, plant extracts, etc.) to enhance their effects in feed.

[0025] Intelligent monitoring technology: Use modern scientific and technological means to monitor and evaluate the application effect of probiotics in feed in real time, so as to adjust the feed formula in time.

[0026] In summary, as an important feed additive, probiotics have many advantages, such as promoting animal growth, enhancing immunity and improving intestinal health. However, the application of probiotic feed additives in the current market still faces some technical challenges. Through in-depth research on the preparation, application and effects of probiotics, combined with modern scientific and technological means, it is expected to further improve the application effect of probiotics in feed and promote the sustainable development of the breeding industry. Summary of the invention

[0027] 1. Problems to be solved

[0028] In view of the problems existing in the above-mentioned prior art, the present invention provides a feed additive containing probiotics and a preparation method thereof. Through the technical scheme of the present invention, a specific coating material and a probiotic combination are adopted to improve the survival rate and stability of the probiotics, while optimizing the nutritional components of the feed, thereby improving the growth performance of animals.

[0029] 2. Technical solution

[0030] To solve the above problems, the present invention adopts the following technical solutions.

[0031] Feed additives containing probiotics,

[0032] In parts by weight, it includes the following components:

[0033] Envelope nuclear membrane 40-70 copies,

[0034] 15-25 copies of envelope core;

[0035] The envelope nuclear membrane is polylactic acid, gelatin, sodium alginate, xanthan gum, glyceride, and citric acid, wherein the usage ratio of polylactic acid, gelatin, sodium alginate, xanthan gum, glyceride, and citric acid is (3-5): (1-2): (2-3): (1-5): (2-6): (1-3);

[0036] The coating core comprises the following components in parts by weight:

[0037]

[0038] The probiotics are freeze-dried powder of Lactobacillus acidophilus and freeze-dried powder of Lactobacillus paracasei, wherein the weight ratio of the freeze-dried powder of Lactobacillus acidophilus to the freeze-dried powder of Lactobacillus paracasei is 1:(2-4);

[0039] The Lactobacillus acidophilus freeze-dried powder has a Latin name of Lactobacillus acidophilus and a collection number of CGMCC 1.12735, and is deposited in the China General Microbiological Culture Collection Center;

[0040] The Lactobacillus paracasei freeze-dried powder has a Latin name of Lactobacillus paracasei and a collection number of CGMCC 1.12731, and is preserved in the China General Microbiological Culture Collection Center.

[0041] Preferably, the above-mentioned feed additive containing probiotics,

[0042] In parts by weight, it includes the following components:

[0043] Envelope nuclear membrane 50-65 copies,

[0044] 18-23 copies of envelope core;

[0045] The envelope nuclear membrane is polylactic acid, gelatin, sodium alginate, xanthan gum, glyceride, and citric acid, wherein the usage ratio of polylactic acid, gelatin, sodium alginate, xanthan gum, glyceride, and citric acid is (3-5): (1-2): (2-3): (1-5): (3-5): (1-3);

[0046] The coating core comprises the following components in parts by weight:

[0047]

[0048]

[0049] The probiotics are freeze-dried powder of Lactobacillus acidophilus and freeze-dried powder of Lactobacillus paracasei, wherein the weight ratio of the freeze-dried powder of Lactobacillus acidophilus to the freeze-dried powder of Lactobacillus paracasei is 1:(2-4);

[0050] The Lactobacillus acidophilus freeze-dried powder has a Latin name of Lactobacillus acidophilus and a collection number of CGMCC 1.12735, and is deposited in the China General Microbiological Culture Collection Center;

[0051] The Lactobacillus paracasei freeze-dried powder has a Latin name of Lactobacillus paracasei and a preservation number of CGMCC 1.12731, and is preserved in the China General Microbiological Culture Collection Center.

[0052] Preferably, the above-mentioned feed additive containing probiotics,

[0053] In parts by weight, it includes the following components:

[0054] 60 copies of nuclear envelope,

[0055] 21 copies of envelope core;

[0056] The envelope nuclear membrane is polylactic acid, gelatin, sodium alginate, xanthan gum, glyceride, and citric acid, wherein the usage ratio of polylactic acid, gelatin, sodium alginate, xanthan gum, glyceride, and citric acid is 4:2:2:3:4:2;

[0057] The coating core comprises the following components in parts by weight:

[0058]

[0059] The probiotics are freeze-dried powder of Lactobacillus acidophilus and freeze-dried powder of Lactobacillus paracasei, wherein the weight ratio of the freeze-dried powder of Lactobacillus acidophilus to the freeze-dried powder of Lactobacillus paracasei is 1:3;

[0060] The Lactobacillus acidophilus freeze-dried powder has a Latin name of Lactobacillus acidophilus and a collection number of CGMCC 1.12735, and is deposited in the China General Microbiological Culture Collection Center;

[0061] The Lactobacillus paracasei freeze-dried powder has a Latin name of Lactobacillus paracasei and a preservation number of CGMCC 1.12731, and is preserved in the China General Microbiological Culture Collection Center.

[0062] Preferably, in the above-mentioned feed additive containing probiotics, the extraction process of the soybean protein is as follows:

[0063] Defatted soybean powder and deionized water are mixed in a weight ratio of 1:10 to obtain a mixed solution, and then the pH value of the mixed solution is adjusted to 8 using a sodium hydroxide solution. The adjusted mixed solution is then stirred and extracted at 50-60°C for 1-2 hours, and the extracted mixed solution is transferred to a centrifuge and centrifuged at a speed of 10000rpm for 15-20 minutes. After centrifugation, the supernatant is taken out, and an HCl solution is slowly added to the supernatant to adjust the pH value to 4.5. The adjusted solution is allowed to stand for 30 minutes, and the precipitate after standing is centrifuged again at a speed of 10000rpm for 15-20 minutes. The supernatant is removed, and the precipitate is added with ten times the mass of deionized water to dissolve, and the pH of the solution is adjusted to 7.0 using a pH meter. The dissolved soybean protein is dried by a vacuum freeze-drying method to obtain dried soybean protein.

[0064] Preferably, in the above-mentioned probiotic-containing feed additive, the variety of rice bran is rice.

[0065] A method for preparing a feed additive containing probiotics comprises the following steps:

[0066] (1) Preparation of nuclear envelope: heat and melt polylactic acid, gelatin, sodium alginate, xanthan gum, glyceride, and citric acid to obtain an envelope solution;

[0067] (2) Preparation of coated core: transfer soybean protein, bean curd residue, rice bran, dry yeast and probiotics into a feed tank, stir and mix thoroughly, and granulate to obtain pellet feed;

[0068] (3) placing the pelleted feed described in step (2) in a fluidized bed for drying;

[0069] (4) Atomizing the coating solution described in step (1) onto the surface of the pellet feed dried in step (3) to obtain.

[0070] Preferably, the method for preparing the above-mentioned feed additive containing probiotics,

[0071] The temperature for heating and dissolving in step (1) is 75°C.

[0072] Preferably, the method for preparing the above-mentioned feed additive containing probiotics,

[0073] The average diameter of the pelleted feed in step (2) is 2 mm.

[0074] Preferably, the method for preparing the above-mentioned feed additive containing probiotics,

[0075] The average moisture content of the pelleted feed after drying in step (3) is 8%.

[0076] 3. Beneficial effects

[0077] Compared with the prior art, the present invention has the following beneficial effects:

[0078] (1) Promote animal growth

[0079] Probiotics improve the balance of intestinal microbiota and inhibit the growth of harmful bacteria, thereby increasing the growth rate and feed utilization of animals.

[0080] (2) Enhance immunity

[0081] Probiotics can stimulate the activity of immune cells in the intestine, enhance the body's nonspecific immune function, and reduce the incidence of disease.

[0082] (3) Improve intestinal health

[0083] By promoting the proliferation of beneficial bacteria and inhibiting the growth of pathogenic bacteria, probiotics help maintain the balance of intestinal microecology and reduce digestive problems such as diarrhea.

[0084] (4) Improve feed conversion rate

[0085] Probiotics can break down complex components in feed, improve the digestion and absorption efficiency of nutrients, and thus improve feed conversion rate.

[0086] (5) Application status and challenges

[0087] At present, the demand for probiotic feed additives in the market is gradually increasing. The main ingredients include lactic acid bacteria, bifidobacteria and yeast, which have achieved remarkable results in a variety of animal feeds. However, the existing products face the problem of decreased number of live bacteria during production, transportation and storage, which affects their effectiveness.

[0088] (6) Future research directions

[0089] Current research focuses include the development of new probiotics, the application of microencapsulation technology, the research of compound additives, and the use of intelligent monitoring technology to improve the application effect of probiotics in feed.

[0090] In summary, feed additives containing probiotics have significant technical effects in promoting animal growth, enhancing immunity and improving intestinal health, but challenges such as stability, survival rate and economy still need to be addressed to promote their sustainable development in the breeding industry. DETAILED DESCRIPTION

[0091] The present invention is further described below in conjunction with specific embodiments.

[0092] Example 1

[0093] The feed additive containing probiotics comprises the following components in parts by weight:

[0094] 40 copies of nuclear envelope,

[0095] 25 copies of envelope core;

[0096] The envelope nuclear membrane is polylactic acid, gelatin, sodium alginate, xanthan gum, glyceride, and citric acid, wherein the usage ratio of polylactic acid, gelatin, sodium alginate, xanthan gum, glyceride, and citric acid is 3:2:2:5:2:3;

[0097] The coating core comprises the following components in parts by weight:

[0098]

[0099] The probiotics are Lactobacillus acidophilus freeze-dried powder and Lactobacillus paracasei freeze-dried powder, wherein the weight ratio of the Lactobacillus acidophilus freeze-dried powder to the Lactobacillus paracasei freeze-dried powder is 1:2;

[0100] The Lactobacillus acidophilus freeze-dried powder has a Latin name of Lactobacillus acidophilus and a collection number of CGMCC 1.12735, and is deposited in the China General Microbiological Culture Collection Center;

[0101] The Lactobacillus paracasei freeze-dried powder has a Latin name of Lactobacillus paracasei and a collection number of CGMCC 1.12731, and is preserved in the China General Microbiological Culture Collection Center.

[0102] Among them, the extraction process of soybean protein involved is as follows:

[0103] Defatted soy flour and deionized water are mixed in a weight ratio of 1:10 to obtain a mixed solution, and then the pH value of the mixed solution is adjusted to 8 using a sodium hydroxide solution. The adjusted mixed solution is then stirred and extracted at 50°C for 2 hours, and the extracted mixed solution is transferred to a centrifuge and centrifuged at a speed of 10000 rpm for 15 minutes. After centrifugation, the supernatant is taken out, and HCl solution is slowly added to the supernatant to adjust the pH value to 4.5. The adjusted solution is allowed to stand for 30 minutes, and the precipitate after standing is centrifuged again at a speed of 10000 rpm for 15 minutes. The supernatant is removed, and the precipitate is added with ten times the mass of deionized water to dissolve it, and the pH of the solution is adjusted to 7.0 using a pH meter. The dissolved soy protein is dried by a vacuum freeze-drying method to obtain dry soy protein.

[0104] It should be noted that the variety of the rice bran is rice.

[0105] The preparation method of the feed additive containing probiotics comprises the following steps:

[0106] (1) Preparation of nuclear envelope: heat and melt polylactic acid, gelatin, sodium alginate, xanthan gum, glyceride, and citric acid to obtain an envelope solution; the heating and dissolving temperature is 75°C;

[0107] (2) Preparation of coated core: transfer soybean protein, bean curd residue, rice bran, dry yeast and probiotics into a feed tank, stir and mix thoroughly, and granulate to obtain pellet feed; the average diameter of the pellet feed is 2 mm;

[0108] (3) placing the pellet feed described in step (2) in a fluidized bed for drying; the average moisture content of the pellet feed after drying is 8%;

[0109] (4) Atomizing the coating solution described in step (1) onto the surface of the pellet feed dried in step (3) to obtain.

[0110] Example 2

[0111] The feed additive containing probiotics comprises the following components in parts by weight:

[0112] 70 copies of envelope nuclear membrane,

[0113] 15 copies of envelope core;

[0114] The envelope nuclear membrane is polylactic acid, gelatin, sodium alginate, xanthan gum, glyceride, and citric acid, wherein the usage ratio of polylactic acid, gelatin, sodium alginate, xanthan gum, glyceride, and citric acid is 5:1:3:1:6:1;

[0115] The coating core comprises the following components in parts by weight:

[0116]

[0117] The probiotics are freeze-dried powder of Lactobacillus acidophilus and freeze-dried powder of Lactobacillus paracasei, wherein the weight ratio of the freeze-dried powder of Lactobacillus acidophilus to the freeze-dried powder of Lactobacillus paracasei is 1:4;

[0118] The Lactobacillus acidophilus freeze-dried powder has a Latin name of Lactobacillus acidophilus and a collection number of CGMCC 1.12735, and is deposited in the China General Microbiological Culture Collection Center;

[0119] The Lactobacillus paracasei freeze-dried powder has a Latin name of Lactobacillus paracasei and a collection number of CGMCC 1.12731, and is preserved in the China General Microbiological Culture Collection Center.

[0120] Among them, the extraction process of soybean protein involved is as follows:

[0121] Defatted soy flour and deionized water are mixed in a weight ratio of 1:10 to obtain a mixed solution, and then the pH value of the mixed solution is adjusted to 8 using a sodium hydroxide solution. The adjusted mixed solution is then stirred and extracted at 60°C for 1 hour, and the extracted mixed solution is transferred to a centrifuge and centrifuged at a speed of 10000 rpm for 20 minutes. After centrifugation, the supernatant is taken out, and HCl solution is slowly added to the supernatant to adjust the pH value to 4.5. The adjusted solution is allowed to stand for 30 minutes, and the precipitate after standing is centrifuged again at a speed of 10000 rpm for 20 minutes. The supernatant is removed, and the precipitate is added with ten times the mass of deionized water to dissolve it, and the pH of the solution is adjusted to 7.0 using a pH meter. The dissolved soy protein is dried by a vacuum freeze-drying method to obtain dry soy protein.

[0122] It should be noted that the variety of the rice bran is rice.

[0123] The preparation method of the feed additive containing probiotics comprises the following steps:

[0124] (1) Preparation of nuclear envelope: heat and melt polylactic acid, gelatin, sodium alginate, xanthan gum, glyceride, and citric acid to obtain an envelope solution; the heating and dissolving temperature is 75°C;

[0125] (2) Preparation of coated core: transfer soybean protein, bean curd residue, rice bran, dry yeast and probiotics into a feed tank, stir and mix thoroughly, and granulate to obtain pellet feed; the average diameter of the pellet feed is 2 mm;

[0126] (3) placing the pellet feed described in step (2) in a fluidized bed for drying; the average moisture content of the pellet feed after drying is 8%;

[0127] (4) Atomizing the coating solution described in step (1) onto the surface of the pellet feed dried in step (3) to obtain.

[0128] Example 3

[0129] The feed additive containing probiotics comprises the following components in parts by weight:

[0130] 50 copies of nuclear envelope,

[0131] 23 copies of envelope core;

[0132] The envelope nuclear membrane is polylactic acid, gelatin, sodium alginate, xanthan gum, glyceride, and citric acid, wherein the usage ratio of polylactic acid, gelatin, sodium alginate, xanthan gum, glyceride, and citric acid is 3:1:2:1:3:1;

[0133] The coating core comprises the following components in parts by weight:

[0134]

[0135]

[0136] The probiotics are Lactobacillus acidophilus freeze-dried powder and Lactobacillus paracasei freeze-dried powder, wherein the weight ratio of the Lactobacillus acidophilus freeze-dried powder to the Lactobacillus paracasei freeze-dried powder is 1:2;

[0137] The Lactobacillus acidophilus freeze-dried powder has a Latin name of Lactobacillus acidophilus and a collection number of CGMCC 1.12735, and is deposited in the China General Microbiological Culture Collection Center;

[0138] The Lactobacillus paracasei freeze-dried powder has a Latin name of Lactobacillus paracasei and a preservation number of CGMCC 1.12731, and is preserved in the China General Microbiological Culture Collection Center.

[0139] Among them, the extraction process of soybean protein involved is as follows:

[0140] Defatted soybean powder and deionized water are mixed in a weight ratio of 1:10 to obtain a mixed solution, and then the pH value of the mixed solution is adjusted to 8 using a sodium hydroxide solution. The adjusted mixed solution is then stirred and extracted at 50°C for 1 hour, and the extracted mixed solution is transferred to a centrifuge and centrifuged at a speed of 10000 rpm for 1 minute. After centrifugation, the supernatant is taken out, and HCl solution is slowly added to the supernatant to adjust the pH value to 4.5. The adjusted solution is allowed to stand for 30 minutes, and the precipitate after standing is centrifuged again at a speed of 10000 rpm for 15 minutes. The supernatant is removed, and the precipitate is added with ten times the mass of deionized water to dissolve it, and the pH of the solution is adjusted to 7.0 using a pH meter. The dissolved soybean protein is dried by a vacuum freeze-drying method to obtain dry soybean protein.

[0141] It should be noted that the variety of the rice bran is rice.

[0142] The preparation method of the feed additive containing probiotics comprises the following steps:

[0143] (1) Preparation of nuclear envelope: heat and melt polylactic acid, gelatin, sodium alginate, xanthan gum, glyceride, and citric acid to obtain an envelope solution; the heating and dissolving temperature is 75°C;

[0144] (2) Preparation of coated core: transfer soybean protein, bean curd residue, rice bran, dry yeast and probiotics into a feed tank, stir and mix thoroughly, and granulate to obtain pellet feed; the average diameter of the pellet feed is 2 mm;

[0145] (3) placing the pellet feed described in step (2) in a fluidized bed for drying; the average moisture content of the pellet feed after drying is 8%;

[0146] (4) Atomizing the coating solution described in step (1) onto the surface of the pellet feed dried in step (3) to obtain.

[0147] Example 4

[0148] The feed additive containing probiotics comprises the following components in parts by weight:

[0149] 65 copies of nuclear envelope,

[0150] 18 copies of envelope core;

[0151] The envelope nuclear membrane is polylactic acid, gelatin, sodium alginate, xanthan gum, glyceride, and citric acid, wherein the usage ratio of polylactic acid, gelatin, sodium alginate, xanthan gum, glyceride, and citric acid is 5:2:3:5:5:3;

[0152] The coating core comprises the following components in parts by weight:

[0153]

[0154] The probiotics are freeze-dried powder of Lactobacillus acidophilus and freeze-dried powder of Lactobacillus paracasei, wherein the weight ratio of the freeze-dried powder of Lactobacillus acidophilus to the freeze-dried powder of Lactobacillus paracasei is 1:4;

[0155] The Lactobacillus acidophilus freeze-dried powder has a Latin name of Lactobacillus acidophilus and a collection number of CGMCC 1.12735, and is deposited in the China General Microbiological Culture Collection Center;

[0156] The Lactobacillus paracasei freeze-dried powder has a Latin name of Lactobacillus paracasei and a preservation number of CGMCC 1.12731, and is preserved in the China General Microbiological Culture Collection Center.

[0157] Among them, the extraction process of soybean protein involved is as follows:

[0158] Defatted soybean powder and deionized water are mixed in a weight ratio of 1:10 to obtain a mixed solution, and then the pH value of the mixed solution is adjusted to 8 using a sodium hydroxide solution. The adjusted mixed solution is then stirred and extracted at 60°C for 2 hours, and the extracted mixed solution is transferred to a centrifuge and centrifuged at a speed of 10000 rpm for 20 minutes. After centrifugation, the supernatant is taken out, and HCl solution is slowly added to the supernatant to adjust the pH value to 4.5. The adjusted solution is allowed to stand for 30 minutes, and the precipitate after standing is centrifuged again at a speed of 10000 rpm for 20 minutes. The supernatant is removed, and the precipitate is added with ten times the mass of deionized water to dissolve it, and the pH of the solution is adjusted to 7.0 using a pH meter. The dissolved soybean protein is dried by a vacuum freeze-drying method to obtain dry soybean protein.

[0159] It should be noted that the variety of the rice bran is rice.

[0160] The preparation method of the feed additive containing probiotics comprises the following steps:

[0161] (1) Preparation of nuclear envelope: heat and melt polylactic acid, gelatin, sodium alginate, xanthan gum, glyceride, and citric acid to obtain an envelope solution; the heating and dissolving temperature is 75°C;

[0162] (2) Preparation of coated core: transfer soybean protein, bean curd residue, rice bran, dry yeast and probiotics into a feed tank, stir and mix thoroughly, and granulate to obtain pellet feed; the average diameter of the pellet feed is 2 mm;

[0163] (3) placing the pellet feed described in step (2) in a fluidized bed for drying; the average moisture content of the pellet feed after drying is 8%;

[0164] (4) Atomizing the coating solution described in step (1) onto the surface of the pellet feed dried in step (3) to obtain.

[0165] Example 5

[0166] The feed additive containing probiotics comprises the following components in parts by weight:

[0167] 60 copies of nuclear envelope,

[0168] 21 copies of envelope core;

[0169] The envelope nuclear membrane is polylactic acid, gelatin, sodium alginate, xanthan gum, glyceride, and citric acid, wherein the usage ratio of polylactic acid, gelatin, sodium alginate, xanthan gum, glyceride, and citric acid is 4:2:2:3:4:2;

[0170] The coating core comprises the following components in parts by weight:

[0171]

[0172] The probiotics are freeze-dried powder of Lactobacillus acidophilus and freeze-dried powder of Lactobacillus paracasei, wherein the weight ratio of the freeze-dried powder of Lactobacillus acidophilus to the freeze-dried powder of Lactobacillus paracasei is 1:3;

[0173] The Lactobacillus acidophilus freeze-dried powder has a Latin name of Lactobacillus acidophilus and a collection number of CGMCC 1.12735, and is deposited in the China General Microbiological Culture Collection Center;

[0174] The Lactobacillus paracasei freeze-dried powder has a Latin name of Lactobacillus paracasei and a preservation number of CGMCC 1.12731, and is preserved in the China General Microbiological Culture Collection Center.

[0175] Among them, the extraction process of soybean protein involved is as follows:

[0176] Defatted soy flour and deionized water are mixed in a weight ratio of 1:10 to obtain a mixed solution, and then the pH value of the mixed solution is adjusted to 8 using a sodium hydroxide solution. The adjusted mixed solution is then stirred and extracted at 55°C for 1.5 hours, and the extracted mixed solution is transferred to a centrifuge and centrifuged at a speed of 10,000 rpm for 16 minutes. After centrifugation, the supernatant is taken out, and HCl solution is slowly added to the supernatant to adjust the pH value to 4.5. The adjusted solution is allowed to stand for 30 minutes, and the precipitate after standing is centrifuged again at a speed of 10,000 rpm for 17 minutes. The supernatant is removed, and the precipitate is added with ten times the mass of deionized water to dissolve it, and the pH of the solution is adjusted to 7.0 using a pH meter. The dissolved soy protein is dried by a vacuum freeze-drying method to obtain dry soy protein.

[0177] It should be noted that the variety of the rice bran is rice.

[0178] The preparation method of the feed additive containing probiotics comprises the following steps:

[0179] (1) Preparation of nuclear envelope: heat and melt polylactic acid, gelatin, sodium alginate, xanthan gum, glyceride, and citric acid to obtain an envelope solution; the heating and dissolving temperature is 75°C;

[0180] (2) Preparation of coated core: transfer soybean protein, bean curd residue, rice bran, dry yeast and probiotics into a feed tank, stir and mix thoroughly, and granulate to obtain pellet feed; the average diameter of the pellet feed is 2 mm;

[0181] (3) placing the pellet feed described in step (2) in a fluidized bed for drying; the average moisture content of the pellet feed after drying is 8%;

[0182] (4) Atomizing the coating solution described in step (1) onto the surface of the pellet feed dried in step (3) to obtain.

[0183] Comparative Example 1

[0184] The feed additive containing probiotics comprises the following components in parts by weight:

[0185] 60 copies of nuclear envelope,

[0186] 21 copies of envelope core;

[0187] The envelope nuclear membrane is gelatin, sodium alginate, xanthan gum, glyceride, and citric acid, wherein the ratio of gelatin, sodium alginate, xanthan gum, glyceride, and citric acid is 2:2:3:4:2;

[0188] The coating core comprises the following components in parts by weight:

[0189]

[0190] The probiotics are freeze-dried powder of Lactobacillus acidophilus and freeze-dried powder of Lactobacillus paracasei, wherein the weight ratio of the freeze-dried powder of Lactobacillus acidophilus to the freeze-dried powder of Lactobacillus paracasei is 1:3;

[0191] The Lactobacillus acidophilus freeze-dried powder has a Latin name of Lactobacillus acidophilus and a collection number of CGMCC 1.12735, and is deposited in the China General Microbiological Culture Collection Center;

[0192] The Lactobacillus paracasei freeze-dried powder has a Latin name of Lactobacillus paracasei and a preservation number of CGMCC 1.12731, and is preserved in the China General Microbiological Culture Collection Center.

[0193] Among them, the extraction process of soybean protein involved is as follows:

[0194] Defatted soy flour and deionized water are mixed in a weight ratio of 1:10 to obtain a mixed solution, and then the pH value of the mixed solution is adjusted to 8 using a sodium hydroxide solution. The adjusted mixed solution is then stirred and extracted at 55°C for 1.5 hours, and the extracted mixed solution is transferred to a centrifuge and centrifuged at a speed of 10,000 rpm for 16 minutes. After centrifugation, the supernatant is taken out, and HCl solution is slowly added to the supernatant to adjust the pH value to 4.5. The adjusted solution is allowed to stand for 30 minutes, and the precipitate after standing is centrifuged again at a speed of 10,000 rpm for 17 minutes. The supernatant is removed, and the precipitate is added with ten times the mass of deionized water to dissolve it, and the pH of the solution is adjusted to 7.0 using a pH meter. The dissolved soy protein is dried by a vacuum freeze-drying method to obtain dry soy protein.

[0195] It should be noted that the variety of the rice bran is rice.

[0196] The preparation method of the feed additive containing probiotics comprises the following steps:

[0197] (1) Preparation of nuclear envelope: heating and melting gelatin, sodium alginate, xanthan gum, glyceride, and citric acid to obtain an envelope solution; the heating and dissolving temperature is 75°C;

[0198] (2) Preparation of coated core: transfer soybean protein, bean curd residue, rice bran, dry yeast and probiotics into a feed tank, stir and mix thoroughly, and granulate to obtain pellet feed; the average diameter of the pellet feed is 2 mm;

[0199] (3) placing the pellet feed described in step (2) in a fluidized bed for drying; the average moisture content of the pellet feed after drying is 8%;

[0200] (4) Atomizing the coating solution described in step (1) onto the surface of the pellet feed dried in step (3) to obtain.

[0201] Comparative Example 2

[0202] The feed additive containing probiotics comprises the following components in parts by weight:

[0203] 60 copies of nuclear envelope,

[0204] 21 copies of envelope core;

[0205] The envelope nuclear membrane is polylactic acid, gelatin, sodium alginate, glyceride, and citric acid, wherein the usage ratio of polylactic acid, gelatin, sodium alginate, glyceride, and citric acid is 4:2:2:4:2;

[0206] The coating core comprises the following components in parts by weight:

[0207]

[0208] The probiotics are freeze-dried powder of Lactobacillus acidophilus and freeze-dried powder of Lactobacillus paracasei, wherein the weight ratio of the freeze-dried powder of Lactobacillus acidophilus to the freeze-dried powder of Lactobacillus paracasei is 1:3;

[0209] The Lactobacillus acidophilus freeze-dried powder has a Latin name of Lactobacillus acidophilus and a collection number of CGMCC 1.12735, and is deposited in the China General Microbiological Culture Collection Center;

[0210] The Lactobacillus paracasei freeze-dried powder has a Latin name of Lactobacillus paracasei and a preservation number of CGMCC 1.12731, and is preserved in the China General Microbiological Culture Collection Center.

[0211] Among them, the extraction process of soybean protein involved is as follows:

[0212] Defatted soy flour and deionized water are mixed in a weight ratio of 1:10 to obtain a mixed solution, and then the pH value of the mixed solution is adjusted to 8 using a sodium hydroxide solution. The adjusted mixed solution is then stirred and extracted at 55°C for 1.5 hours, and the extracted mixed solution is transferred to a centrifuge and centrifuged at a speed of 10,000 rpm for 16 minutes. After centrifugation, the supernatant is taken out, and HCl solution is slowly added to the supernatant to adjust the pH value to 4.5. The adjusted solution is allowed to stand for 30 minutes, and the precipitate after standing is centrifuged again at a speed of 10,000 rpm for 17 minutes. The supernatant is removed, and the precipitate is added with ten times the mass of deionized water to dissolve it, and the pH of the solution is adjusted to 7.0 using a pH meter. The dissolved soy protein is dried by a vacuum freeze-drying method to obtain dry soy protein.

[0213] It should be noted that the variety of the rice bran is rice.

[0214] The preparation method of the feed additive containing probiotics comprises the following steps:

[0215] (1) Preparation of nuclear envelope: heat and melt polylactic acid, gelatin, sodium alginate, glyceride, and citric acid to obtain an envelope solution; the heating and dissolving temperature is 75°C;

[0216] (2) Preparation of coated core: transfer soybean protein, bean curd residue, rice bran, dry yeast and probiotics into a feed tank, stir and mix thoroughly, and granulate to obtain pellet feed; the average diameter of the pellet feed is 2 mm;

[0217] (3) placing the pellet feed described in step (2) in a fluidized bed for drying; the average moisture content of the pellet feed after drying is 8%;

[0218] (4) Atomizing the coating solution described in step (1) onto the surface of the pellet feed dried in step (3) to obtain.

[0219] Comparative Example 3

[0220] The feed additive containing probiotics comprises the following components in parts by weight:

[0221] 60 copies of nuclear envelope,

[0222] 21 copies of envelope core;

[0223] The envelope nuclear membrane is polylactic acid, gelatin, sodium alginate, xanthan gum, glyceride, and citric acid, wherein the usage ratio of polylactic acid, gelatin, sodium alginate, xanthan gum, glyceride, and citric acid is 4:2:2:3:4:2;

[0224] The coating core comprises the following components in parts by weight:

[0225]

[0226] The probiotics are freeze-dried powder of Lactobacillus acidophilus and freeze-dried powder of Lactobacillus paracasei, wherein the weight ratio of the freeze-dried powder of Lactobacillus acidophilus to the freeze-dried powder of Lactobacillus paracasei is 1:3;

[0227] The Lactobacillus acidophilus freeze-dried powder has a Latin name of Lactobacillus acidophilus and a collection number of CGMCC 1.12735, and is deposited in the China General Microbiological Culture Collection Center;

[0228] The Lactobacillus paracasei freeze-dried powder has a Latin name of Lactobacillus paracasei and a preservation number of CGMCC 1.12731, and is preserved in the China General Microbiological Culture Collection Center.

[0229] It should be noted that the variety of the rice bran is rice.

[0230] The preparation method of the feed additive containing probiotics comprises the following steps:

[0231] (1) Preparation of nuclear envelope: heat and melt polylactic acid, gelatin, sodium alginate, xanthan gum, glyceride, and citric acid to obtain an envelope solution; the heating and dissolving temperature is 75°C;

[0232] (2) Preparation of coated core: transfer the tofu dregs, rice bran, dry yeast and probiotics into a feed tank, stir and mix thoroughly, and granulate to obtain pellet feed; the average diameter of the pellet feed is 2 mm;

[0233] (3) placing the pellet feed described in step (2) in a fluidized bed for drying; the average moisture content of the pellet feed after drying is 8%;

[0234] (4) Atomizing the coating solution described in step (1) onto the surface of the pellet feed dried in step (3) to obtain.

[0235] Comparative Example 4

[0236] The feed additive containing probiotics comprises the following components in parts by weight:

[0237] 60 copies of nuclear envelope,

[0238] 21 copies of envelope core;

[0239] The envelope nuclear membrane is polylactic acid, gelatin, sodium alginate, xanthan gum, glyceride, and citric acid, wherein the usage ratio of polylactic acid, gelatin, sodium alginate, xanthan gum, glyceride, and citric acid is 4:2:2:3:4:2;

[0240] The coating core comprises the following components in parts by weight:

[0241]

[0242] The probiotics are freeze-dried powder of Lactobacillus acidophilus and freeze-dried powder of Lactobacillus paracasei, wherein the weight ratio of the freeze-dried powder of Lactobacillus acidophilus to the freeze-dried powder of Lactobacillus paracasei is 1:3;

[0243] The Lactobacillus acidophilus freeze-dried powder has a Latin name of Lactobacillus acidophilus and a collection number of CGMCC 1.12735, and is deposited in the China General Microbiological Culture Collection Center;

[0244] The Lactobacillus paracasei freeze-dried powder has a Latin name of Lactobacillus paracasei and a preservation number of CGMCC 1.12731, and is preserved in the China General Microbiological Culture Collection Center.

[0245] Among them, the extraction process of soybean protein involved is as follows:

[0246] Defatted soy flour and deionized water are mixed in a weight ratio of 1:10 to obtain a mixed solution, and then the pH value of the mixed solution is adjusted to 8 using a sodium hydroxide solution. The adjusted mixed solution is then stirred and extracted at 55°C for 1.5 hours, and the extracted mixed solution is transferred to a centrifuge and centrifuged at a speed of 10,000 rpm for 16 minutes. After centrifugation, the supernatant is taken out, and HCl solution is slowly added to the supernatant to adjust the pH value to 4.5. The adjusted solution is allowed to stand for 30 minutes, and the precipitate after standing is centrifuged again at a speed of 10,000 rpm for 17 minutes. The supernatant is removed, and the precipitate is added with ten times the mass of deionized water to dissolve it, and the pH of the solution is adjusted to 7.0 using a pH meter. The dissolved soy protein is dried by a vacuum freeze-drying method to obtain dry soy protein.

[0247] It should be noted that the variety of the rice bran is rice.

[0248] The preparation method of the feed additive containing probiotics comprises the following steps:

[0249] (1) Preparation of nuclear envelope: heat and melt polylactic acid, gelatin, sodium alginate, xanthan gum, glyceride, and citric acid to obtain an envelope solution; the heating and dissolving temperature is 75°C;

[0250] (2) Preparation of coated core: transfer soybean protein, bean curd residue, rice bran and probiotics into a feed tank, stir and mix thoroughly, and granulate to obtain pellet feed; the average diameter of the pellet feed is 2 mm;

[0251] (3) placing the pellet feed described in step (2) in a fluidized bed for drying; the average moisture content of the pellet feed after drying is 8%;

[0252] (4) Atomizing the coating solution described in step (1) onto the surface of the pellet feed dried in step (3) to obtain.

[0253] Comparative Example 5

[0254] Feed additives, in parts by weight, include the following components:

[0255] 60 copies of nuclear envelope,

[0256] 21 copies of envelope core;

[0257] The envelope nuclear membrane is polylactic acid, gelatin, sodium alginate, xanthan gum, glyceride, and citric acid, wherein the usage ratio of polylactic acid, gelatin, sodium alginate, xanthan gum, glyceride, and citric acid is 4:2:2:3:4:2;

[0258] The coating core comprises the following components in parts by weight:

[0259]

[0260] Among them, the extraction process of soybean protein involved is as follows:

[0261] Defatted soy flour and deionized water are mixed in a weight ratio of 1:10 to obtain a mixed solution, and then the pH value of the mixed solution is adjusted to 8 using a sodium hydroxide solution. The adjusted mixed solution is then stirred and extracted at 55°C for 1.5 hours, and the extracted mixed solution is transferred to a centrifuge and centrifuged at a speed of 10,000 rpm for 16 minutes. After centrifugation, the supernatant is taken out, and HCl solution is slowly added to the supernatant to adjust the pH value to 4.5. The adjusted solution is allowed to stand for 30 minutes, and the precipitate after standing is centrifuged again at a speed of 10,000 rpm for 17 minutes. The supernatant is removed, and the precipitate is added with ten times the mass of deionized water to dissolve it, and the pH of the solution is adjusted to 7.0 using a pH meter. The dissolved soy protein is dried by a vacuum freeze-drying method to obtain dry soy protein.

[0262] It should be noted that the variety of the rice bran is rice.

[0263] The preparation method of the feed additive comprises the following steps:

[0264] (1) Preparation of nuclear envelope: heat and melt polylactic acid, gelatin, sodium alginate, xanthan gum, glyceride, and citric acid to obtain an envelope solution; the heating and dissolving temperature is 75°C;

[0265] (2) Preparation of coated core: transfer soybean protein, bean curd residue, rice bran and dry yeast into a feed tank, stir and mix thoroughly, and granulate to obtain pellet feed; the average diameter of the pellet feed is 2 mm;

[0266] (3) placing the pellet feed described in step (2) in a fluidized bed for drying; the average moisture content of the pellet feed after drying is 8%;

[0267] (4) Atomizing the coating solution described in step (1) onto the surface of the pellet feed dried in step (3) to obtain.

[0268] Example 6

[0269] The feed additives prepared in Examples 1-5 and Comparative Examples 1-5 were tested:

[0270] Reference test method - (1) Chinese invention patent, application number: CN201910790774.1, publication number: CN110583857A, discloses a feed for enhancing the digestion of suckling pigs and its processing technology;

[0271] (2) Chinese invention patent, application number: CN201910008357.7, publication number: CN109497312A, discloses a feed for improving the digestive tract condition of sows and a preparation method thereof.

[0272] 220 pigs (piglets of grown-up dual-purpose sows) were divided into eleven groups according to the principle of age, weight, health status and gender ratio. The treatment group was mixed with traditional pig feed at a mass percentage of 10%, and the control group was fed with traditional pig feed (522 type extruded compound feed, Huaibei Cargill Agriculture and Animal Husbandry Technology Co., Ltd.). The test period was 2 months, and the test results are as follows:

[0273] Table 1

[0274] Feed to meat ratio Average daily weight gain (kg) Disease rate (%) Example 1 1.6 0.523 2 Example 2 1.5 0.529 2 Example 3 1.3 0.534 2 Example 4 1.3 0.541 1 Example 5 1.2 0.547 1 Comparative Example 1 1.8 0.491 5 Comparative Example 2 2.0 0.480 10 Comparative Example 3 2.1 0.457 15 Comparative Example 4 2.3 0.410 20 Comparative Example 5 2.7 0.346 30 Control group 3.2 0.332 45

[0275] In summary, the present invention provides a feed additive containing probiotics and a preparation method thereof, which has significant innovation and practicality. The feed additive combines a variety of bioactive ingredients through a scientific and reasonable formula design, giving full play to the advantages of probiotics in promoting animal growth, improving feed conversion rate and improving intestinal health. First, the design of the envelope core membrane adopts multiple components such as polylactic acid, gelatin, sodium alginate, xanthan gum, glyceride and citric acid, which can not only form a stable membrane structure, but also achieve a sustained release effect, ensuring the survival rate and activity of probiotics in the intestine. By adjusting the thickness of the envelope and the ratio of the material, the present invention can effectively control the release rate of probiotics, thereby improving its utilization efficiency in the animal body. Secondly, the design of the envelope core combines multiple components such as soy protein, bean curd residue, rice bran, dry yeast and probiotics. This multi-component combination not only reduces the problem of envelope rupture caused by a single component, but also can stimulate the gastric secretion of pigs and promote digestion. In particular, the addition of amino acid liquid meets the needs of animals for basic nutrients, while the combination of brewer's grains and yeast powder further improves the fermentation effect of the feed and enhances the effective release of nutrients. Through the verification of the embodiments, the results show that the prepared feed additive can effectively improve the comprehensive quality of pork, significantly reduce the feed-to-meat ratio, increase the average daily weight gain, and reduce the incidence of illness. These results fully demonstrate the effectiveness and practicality of the present invention and provide a new solution for the breeding industry. Finally, with the continuous improvement of requirements for animal health and meat quality, feed additives containing probiotics will play an increasingly important role. In the future, with the continuous advancement of technology and in-depth research, it is expected that more innovative products and applications will appear in this field, making greater contributions to the sustainable development of the breeding industry.

[0276] In Comparative Example 1, the performance and function of the envelope nuclear membrane will be significantly affected by removing polylactic acid from the envelope nuclear membrane. Polylactic acid (PLA), as a biodegradable polymer, has good film-forming properties and biocompatibility, and plays a key role in feed additives. The following is a mechanism analysis after removing polylactic acid: polylactic acid provides structural stability in the envelope nuclear membrane. After removing polylactic acid, the remaining components (such as gelatin, sodium alginate, xanthan gum, glyceride and citric acid) cannot form a membrane structure of the same strength. This will lead to: membrane fragility: the envelope becomes more fragile and easily breaks during handling and storage, thereby affecting the protective effect of probiotics. Membrane permeability: the presence of polylactic acid helps to regulate the permeability of the membrane, and removal causes the membrane to change in permeability to moisture and gas, affecting the survival of probiotics. The removal of polylactic acid will directly affect the sustained-release performance of the envelope. The characteristics of polylactic acid enable it to slowly release the components in the envelope under specific conditions. After removal, the following situations occur: Accelerated release rate: The absence of polylactic acid leads to an accelerated release rate of probiotics and other nutrients, which cannot be continuously released in the animal intestine, reducing their bioavailability. Waste of nutrients: The rapid release of probiotics and nutrients in the intestine leads to their failure to be fully absorbed, resulting in nutrient waste. The survival rate of probiotics is the key to the effect of feed additives. The removal of polylactic acid leads to: Weakened protective effect: Polylactic acid can provide certain protection for probiotics and reduce their loss in an acidic environment. After removal, the survival rate of probiotics decreases under the influence of gastric acid and digestive enzymes. Decreased number of live bacteria: As the survival rate decreases, the number of live bacteria that eventually reach the intestine decreases, thus affecting its positive effect on animal health. After removing polylactic acid, the interaction of other components in the envelope core membrane will change: Compatibility of components: The compatibility of components such as gelatin and sodium alginate is insufficient, resulting in a decrease in the overall performance of the envelope. Release of functional ingredients: The removal of polylactic acid affects the release characteristics of other functional ingredients (such as glycerides and citric acid), which in turn affects the overall effect of the feed. After the removal of polylactic acid, the structural stability of the envelope core membrane, the sustained release effect, the survival rate of probiotics and the interaction of other ingredients will be significantly affected. These changes lead to a decrease in the overall effect of the feed additive, reducing its role in improving animal growth performance and improving intestinal health. Therefore, when designing feed additives containing probiotics, the use of polylactic acid is indispensable and its importance in the formulation must be carefully considered.

[0277] In comparative example 2, the performance and function of the nuclear membrane of the envelope are significantly affected by removing xanthan gum. Xanthan gum is a natural polysaccharide widely used in food and feed industry, mainly because of its good thickening and stability. The following is the mechanism analysis after removing xanthan gum: xanthan gum plays a role of thickening and stabilization in the nuclear membrane of the envelope. After removing xanthan gum, the overall structure of the envelope is affected by the following: the viscosity of the film is reduced: the removal of xanthan gum causes the viscosity of the envelope liquid to be reduced, thereby affecting the formation and stability of the film. This causes the film to be easily broken or lose shape during drying and storage. The uniformity of the film decreases: xanthan gum helps to improve the uniformity of the envelope, and the thickness of the film is uneven after removal, which affects its performance in the intestinal tract. The presence of xanthan gum helps to achieve a sustained release effect, which causes after removal: the release rate is accelerated: the sustained release performance of the envelope is affected, resulting in an accelerated release rate of probiotics and other nutrients, which cannot be continuously released in the intestinal tract of animals, reducing its bioavailability. Waste of nutrients: Due to the accelerated release rate, probiotics and nutrients are not fully absorbed in the intestines, resulting in nutrient waste. The survival rate of probiotics is the key to the effect of feed additives. After removing xanthan gum, it leads to: Weakened protective effect: Xanthan gum can provide certain protection for probiotics and reduce their losses in acidic environments. After removal, the survival rate of probiotics decreases under the influence of gastric acid and digestive enzymes. Decrease in the number of live bacteria: As the survival rate decreases, the number of live bacteria that eventually reach the intestines decreases, thus affecting their positive effects on animal health. After removing xanthan gum, the interaction between other components in the envelope nuclear membrane will change: Compatibility of components: Removal of xanthan gum leads to insufficient compatibility of other components (such as gelatin, sodium alginate, etc.), affecting the overall performance of the envelope. Release of functional ingredients: The removal of xanthan gum affects the release characteristics of other functional ingredients (such as glycerides and citric acid), thereby affecting the overall effect of the feed. After the removal of xanthan gum, the structural stability of the envelope core membrane, the sustained release effect, the survival rate of probiotics and the interaction of other components will be significantly affected. These changes lead to a decrease in the overall effect of the feed additive, reducing its role in improving animal growth performance and improving intestinal health. Therefore, when designing feed additives containing probiotics, the use of xanthan gum is indispensable and its importance in the formulation must be carefully considered.

[0278] In comparative example 3, the coating core removes soy protein, and the performance and function of the coating core will be significantly affected. As an important nutrient, the removal of soy protein will lead to the following changes in the mechanism: soy protein is one of the main components in the coating core, providing rich amino acids and proteins. After removing soy protein, it leads to: insufficient supply of amino acids: soy protein contains a variety of essential amino acids, and removal will cause a significant decrease in the content of amino acids in feed, thereby affecting animal growth and development. Nutritional imbalance: the removal of soy protein leads to an imbalance in the relative proportion of other nutrients (such as vitamins and minerals) in the feed, affecting the overall nutritional status of the animal. Soy protein not only provides nutrition in the coating core, but also has the effect of promoting digestion. After removal, the following occurs: decreased digestion efficiency: the lack of soy protein leads to a decrease in the digestion efficiency of the feed, and the animal's absorption capacity of other ingredients in the feed decreases. Intestinal health problems: lack of soy protein leads to a decrease in the beneficial flora in the intestine, affecting intestinal health and increasing the risk of indigestion and diarrhea. Soy protein plays a certain structural support role in the coating core. Removal results in: Structural fragility: The overall structure of the coated core becomes more fragile and is prone to rupture during processing and storage, affecting its functionality. Changes in release characteristics: The removal of soy protein causes changes in the release characteristics of the coated core for other ingredients, affecting the effective release of nutrients. Removal of soy protein affects the survival and activity of probiotics: Weakened protective effect: Soy protein can provide certain protection for probiotics, and after removal, the survival rate of probiotics under the influence of gastric acid and digestive enzymes decreases. Decreased number of live bacteria: As the survival rate decreases, the number of live bacteria that eventually reach the intestine decreases, thus affecting its positive effect on animal health. After removing soy protein, the interaction of other components in the coated core will be affected: Compatibility of ingredients: Removal of soy protein leads to insufficient compatibility of other ingredients (such as tofu dregs, rice bran, dry yeast, etc.), affecting the overall performance of the coated core. Effective release of nutrients: The removal of soy protein affects the release characteristics of other nutrients, thereby affecting the overall effect of the feed. Removing soy protein from the coated core will have a significant impact on the overall performance of the feed additive. This includes the loss of nutrients, reduced digestibility, fragility of the core structure, decreased survival of probiotics, and changes in the interaction of other components. These changes lead to a decrease in the effectiveness of feed additives, thereby affecting the growth performance and health of animals. Therefore, when designing feed additives containing probiotics, the use of soy protein is indispensable and its importance in the formulation must be carefully considered.

[0279] In comparative example 4, the coating core removes dry yeast, and the performance and function of the coating core will be significantly affected. Dry yeast not only provides nutrients in feed additives, but also has multiple physiological functions. The following is a mechanism analysis after the removal of dry yeast: Dry yeast is a component rich in protein, vitamins and minerals. Its removal leads to the following effects: Lack of amino acids and vitamins: Dry yeast is rich in amino acids, B vitamins and other nutrients. After removal, these important nutrients in the feed are missing, affecting the growth and health of animals. Nutritional imbalance: The removal of dry yeast leads to an imbalance in the relative proportions of other ingredients in the feed, affecting the overall nutritional status of the animal. Dry yeast not only provides nutrition in the coating core, but also has the effect of promoting digestion. After removal, the following occurs: Digestion efficiency decreases: Dry yeast can promote the growth of beneficial bacteria in the intestine. After removal, digestion efficiency decreases, and the animal's ability to absorb other ingredients in the feed decreases. Intestinal health problems: Lack of dry yeast leads to a decrease in the beneficial flora in the intestine, affecting intestinal health and increasing the risk of indigestion and diarrhea. The removal of dry yeast affects the survival and activity of probiotics: Weakened protective effect: Dry yeast can provide certain protection for probiotics. After removal, the survival rate of probiotics under the influence of gastric acid and digestive enzymes decreases. The number of live bacteria decreases: As the survival rate decreases, the number of live bacteria that eventually reach the intestine decreases, thus affecting its positive effect on animal health. Dry yeast plays a certain structural support role in the coated core. After removal, it leads to: Structural fragility: The overall structure of the coated core becomes more fragile and easily breaks during processing and storage, affecting its functionality. Changes in release characteristics: The removal of dry yeast causes changes in the release characteristics of the coated core to other components, affecting the effective release of nutrients. After the removal of dry yeast, the interaction of other components in the coated core will be affected: Compatibility of components: The removal of dry yeast leads to insufficient compatibility of other components (such as soy protein, tofu residue, rice bran, etc.), affecting the overall performance of the coated core. Effective release of nutrients: The removal of dry yeast affects the release characteristics of other nutrients, thereby affecting the overall effect of the feed. Removing dry yeast from the coated core will have a significant impact on the overall performance of the feed additive. This includes loss of nutrients, reduced digestibility, decreased survival of probiotics, fragility of the coated core structure, and changes in the interaction of other components. These changes lead to a decrease in the effectiveness of the feed additive, thereby affecting the growth performance and health of the animal. Therefore, when designing feed additives containing probiotics, the use of dry yeast is indispensable and its importance in the formulation must be carefully considered.

[0280] In comparative example 5, the coating core removes probiotics, and the performance and function of the overall feed additive will be significantly affected. Probiotics play an important role in feed additives. After removal, the following mechanism changes occur: Probiotics are a type of living microorganisms that can have a beneficial effect on the host. After the probiotics are removed, it leads to: the lack of amino acids and vitamins: Although probiotics themselves do not provide direct amino acids, they indirectly improve the absorption and utilization of amino acids by promoting the growth of beneficial bacteria in the intestine. After removal, animals cannot obtain enough amino acids and vitamins, affecting their growth and health. Nutritional imbalance: The removal of probiotics leads to an imbalance in the relative proportion of other nutrients in the feed, affecting the overall nutritional status of the animal, leading to slow growth or health problems. Probiotics not only provide nutrition in the coating core, but also have the effect of promoting digestion. After removal, the following occurs: Digestion efficiency decreases: Probiotics can promote the proliferation of beneficial bacteria in the intestine and improve the digestive environment by inhibiting the growth of harmful bacteria. After removal, the digestion efficiency decreases and the animal's ability to absorb other ingredients in the feed decreases. Intestinal health problems: Lack of probiotics leads to an imbalance of microbiota in the intestine, increases the number of harmful bacteria, and thus affects intestinal health, increasing the risk of indigestion and diarrhea. Probiotics play an important role in enhancing the immune function of animals. Removal results in: Reduced immunity: Probiotics enhance the body's immune response by stimulating the activity of immune cells in the intestine. After removal, the immunity of animals decreases and they are more susceptible to diseases. Increased illness rate: Due to the decline in immune function, animals are more susceptible to pathogens, resulting in an increase in illness rate and increased breeding costs. The removal of probiotics will directly affect the overall performance of the coated core: Reduced structural stability: The presence of probiotics helps maintain the structural stability of the coated core. After removal, the coated core becomes more fragile and easily breaks during processing and storage, affecting its functionality. Changes in release characteristics: The removal of probiotics causes changes in the release characteristics of the coated core to other components, affecting the effective release of nutrients and reducing the overall effect of the feed. After the probiotics are removed, the interactions of other ingredients in the coated core will be affected: Compatibility of ingredients: The removal of probiotics leads to insufficient compatibility of other ingredients (such as soy protein, tofu dregs, rice bran, dry yeast, etc.), affecting the overall performance of the coated core. Effective release of nutrients: The removal of probiotics affects the release characteristics of other nutrients, which in turn affects the overall effect of the feed. Removing probiotics from the coated core will have a significant impact on the overall performance of the feed additive. This includes the loss of nutrients, reduced digestion ability, decreased immune function, fragility of the coated core structure, and changes in the interactions of other ingredients. These changes lead to a decrease in the effectiveness of the feed additive, thereby affecting the growth performance and health of the animal. Therefore, when designing feed additives containing probiotics, the use of probiotics is indispensable, and its importance in the formulation must be carefully considered.

[0281] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above and that the present invention can be implemented in other specific forms without departing from the spirit or essential features of the present invention. Therefore, the embodiments should be considered exemplary and non-restrictive in all respects, and the scope of the present invention is defined by the appended claims rather than the above description, and it is intended that all changes falling within the meaning and scope of the equivalent elements of the claims be included in the present invention.

[0282] In addition, it should be understood that although the present specification is described according to implementation modes, not every implementation mode contains only one independent technical solution. This description of the specification is only for the sake of clarity. Those skilled in the art should regard the specification as a whole. The technical solutions in each embodiment may also be appropriately combined to form other implementation modes that can be understood by those skilled in the art.

Claims

1. A feed additive containing probiotics, characterized in that: In parts by weight, it includes the following components: Envelope nuclear membrane 40-70 copies, 15-25 copies of envelope core; The envelope nuclear membrane is polylactic acid, gelatin, sodium alginate, xanthan gum, glyceride, and citric acid, wherein the usage ratio of polylactic acid, gelatin, sodium alginate, xanthan gum, glyceride, and citric acid is (3-5): (1-2): (2-3): (1-5): (2-6): (1-3); The coating core described herein comprises the following components in parts by weight: The probiotics are freeze-dried powder of Lactobacillus acidophilus and freeze-dried powder of Lactobacillus paracasei, wherein the weight ratio of the freeze-dried powder of Lactobacillus acidophilus to the freeze-dried powder of Lactobacillus paracasei is 1:(2-4); The Lactobacillus acidophilus freeze-dried powder has a Latin name of Lactobacillus acidophilus and a collection number of CGMCC 1.12735, and is deposited in the China General Microbiological Culture Collection Center; The Lactobacillus paracasei freeze-dried powder has a Latin name of Lactobacillus paracasei and a collection number of CGMCC1.12731, and is preserved in the China General Microbiological Culture Collection Center.

2. The probiotic-containing feed additive according to claim 1, characterized in that: In parts by weight, it includes the following components: Envelope nuclear membrane 50-65 copies, 18-23 copies of envelope core; The envelope nuclear membrane is polylactic acid, gelatin, sodium alginate, xanthan gum, glyceride, and citric acid, wherein the usage ratio of polylactic acid, gelatin, sodium alginate, xanthan gum, glyceride, and citric acid is (3-5): (1-2): (2-3): (1-5): (3-5): (1-3); The coating core described herein comprises the following components in parts by weight: The probiotics are freeze-dried powder of Lactobacillus acidophilus and freeze-dried powder of Lactobacillus paracasei, wherein the weight ratio of the freeze-dried powder of Lactobacillus acidophilus to the freeze-dried powder of Lactobacillus paracasei is 1:(2-4); The Lactobacillus acidophilus freeze-dried powder has a Latin name of Lactobacillus acidophilus and a collection number of CGMCC 1.12735, and is deposited in the China General Microbiological Culture Collection Center; The Lactobacillus paracasei freeze-dried powder has a Latin name of Lactobacillus paracasei and a preservation number of CGMCC 1.12731, and is preserved in the China General Microbiological Culture Collection Center.

3. The probiotic-containing feed additive according to claim 2, characterized in that: In parts by weight, it includes the following components: 60 copies of nuclear envelope, 21 copies of envelope core; The envelope nuclear membrane is polylactic acid, gelatin, sodium alginate, xanthan gum, glyceride, and citric acid, wherein the usage ratio of polylactic acid, gelatin, sodium alginate, xanthan gum, glyceride, and citric acid is 4:2:2:3:4:2; The coating core described herein comprises the following components in parts by weight: The probiotics are freeze-dried powder of Lactobacillus acidophilus and freeze-dried powder of Lactobacillus paracasei, wherein the weight ratio of the freeze-dried powder of Lactobacillus acidophilus to the freeze-dried powder of Lactobacillus paracasei is 1:3; The Lactobacillus acidophilus freeze-dried powder has a Latin name of Lactobacillus acidophilus and a collection number of CGMCC 1.12735, and is deposited in the China General Microbiological Culture Collection Center; The Lactobacillus paracasei freeze-dried powder has a Latin name of Lactobacillus paracasei and a preservation number of CGMCC 1.12731, and is preserved in the China General Microbiological Culture Collection Center.

4. The probiotic-containing feed additive according to claim 3, characterized in that: The extraction process of the soy protein is as follows: Defatted soybean powder and deionized water are mixed in a weight ratio of 1:10 to obtain a mixed solution, and then the pH value of the mixed solution is adjusted to 8 using a sodium hydroxide solution. The adjusted mixed solution is then stirred and extracted at 50-60°C for 1-2 hours, and the extracted mixed solution is transferred to a centrifuge and centrifuged at a speed of 10000rpm for 15-20 minutes. After centrifugation, the supernatant is taken out, and an HCl solution is slowly added to the supernatant to adjust the pH value to 4.

5. The adjusted solution is allowed to stand for 30 minutes, and the precipitate after standing is centrifuged again at a speed of 10000rpm for 15-20 minutes. The supernatant is removed, and the precipitate is added with ten times the mass of deionized water to dissolve, and the pH of the solution is adjusted to 7.0 using a pH meter. The dissolved soybean protein is dried by a vacuum freeze-drying method to obtain dried soybean protein.

5. The probiotic-containing feed additive according to claim 3, characterized in that: The variety of the rice bran is rice.

6. A method for preparing a feed additive containing probiotics, characterized in that: The following steps are involved: (1) Preparation of nuclear envelope: heat and melt polylactic acid, gelatin, sodium alginate, xanthan gum, glyceride, and citric acid to obtain an envelope solution; (2) Preparation of coated core: transfer soybean protein, bean curd residue, rice bran, dry yeast and probiotics into a feed tank, stir and mix thoroughly, and granulate to obtain pellet feed; (3) placing the pelleted feed described in step (2) in a fluidized bed for drying; (4) Atomizing the coating solution described in step (1) onto the surface of the pellet feed dried in step (3) to obtain.

7. The method for preparing a feed additive containing probiotics according to claim 6, characterized in that: The temperature for heating and dissolving in step (1) is 75°C.

8. The method for preparing a feed additive containing probiotics according to claim 6, characterized in that: The average diameter of the pelleted feed in step (2) is 2 mm.

9. The method for preparing a feed additive containing probiotics according to claim 6, characterized in that: The average moisture content of the pelleted feed after drying in step (3) is 8%.

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