Anti-aging, beauty-maintaining and young-keeping health-care food and preparation method thereof

Abstract

The invention discloses an anti-aging, beauty-maintaining and young-keeping health-care food and a preparation method thereof, relates to the technical field of food processing, and aims to solve the technical problems that existing ergothioneine is limited in source and low in purity, and the anti-aging, beauty-maintaining and young-keeping effects of the ergothioneine are difficult to comprehensively exert. The ergothioneine is sourced from natural edible mushrooms, microbial fermentation products or a combination thereof, the ergothioneine is subjected to purification process treatment after extraction or fermentation, the purity of the ergothioneine reaches 95% or above, and the ergothioneine has efficient antioxidant capacity, can effectively remove various free radicals in the body and protect the cell structure and functional integrity, and can be used for preparing the health-care food. The effective amount of ergothioneine in every 100 g of the health food is 5-50 mg, and collagen tripeptide and marine fish oligopeptide are used as auxiliary active ingredients. The preparation method has the advantages that the purity of ergothioneine is improved, the collagen tripeptide contains a specific high-proportion glycine-proline-hydroxyproline (GPH) structure, and the effects of resisting aging, maintaining beauty and keeping young are achieved.

Description

Technical Field

[0001] The invention relates to the technical field of food processing, and more specifically to an anti-aging, beauty and skin care health food and a preparation method thereof. Background Art

[0002] With the improvement of people's living standards and the pursuit of health and beauty, the market demand for anti-aging and beauty products is growing. In the field of health food, there are currently many related products, but they all have certain limitations.

[0003] There are many health foods with antioxidant functions on the market. Common antioxidants such as vitamin C and vitamin E have certain antioxidant capabilities, but there are many problems. Vitamin C has poor stability and is easily affected by environmental factors such as light, temperature, and humidity, causing oxidation and decomposition. Its action time in the body is relatively short. Vitamin E has limited bioavailability and is not strong enough to scavenge some specific free radicals.

[0004] For thioneine products, there are the following defects in its application in health food at present. On the one hand, the source of thioneine is limited and the purity is not high. When some thioneine is extracted from natural edible fungi, the extraction process is relatively rough, resulting in high impurity content and unstable effective ingredient content. In the process of obtaining thioneine from microbial fermentation, the fermentation strain is not optimized, and the fermentation condition control is imprecise, making thioneine low in yield and high in cost. On the other hand, the formula of thioneine in the product is single, often relying only on its own antioxidant capacity, without cooperating with other synergistic ingredients, it is difficult to fully exert its anti-aging and beauty and skin care effects, in view of this, we propose a kind of anti-aging, beauty and skin care health food and its preparation method. Summary of the invention

[0005] The object of the present invention is to provide an anti-aging, beauty and skin care health food and a preparation method thereof, so as to solve the technical problem that the existing ergothioneine has limited sources and low purity, and is difficult to fully exert its anti-aging and beauty and skin care effects.

[0006] In order to solve the above technical problems, the present invention provides the following technical solutions:

[0007] An anti-aging and beauty health food, comprising thioneine as a core active ingredient, wherein the source of thioneine includes natural edible fungi, microbial fermentation products or a combination thereof, and the purity of thioneine reaches more than 95% after extraction or fermentation and purification process, and the food has high-efficiency antioxidant capacity, can effectively remove various free radicals in the body, and protect the integrity of cell structure and function, and the effective amount of thioneine in every 100 grams of the health food is 5 mg to 50 mg;

[0008] With collagen tripeptide and marine fish oligopeptide as auxiliary active ingredients, collagen tripeptide contains a specific high proportion of glycine-proline-hydroxyproline (GPH) structure, extracted from high-quality collagen source through targeted enzymatic hydrolysis technology, with its molecular weight precisely controlled between 279.3 and 294.4, and the GPH content is not less than 3.0%;

[0009] Marine fish oligopeptides are made from deep-sea pollution-free cod skin and refined through a unique bio-enzymatic hydrolysis process. They are highly stable and easy to absorb, with an average molecular weight of less than 1000Da, and the content of oligopeptides with a molecular weight below 500Da is not less than 80%;

[0010] The three ingredients work synergistically to achieve anti-aging and beauty-enhancing effects by regulating the synthesis and distribution of collagen in the human body, increasing skin moisture content, reducing transepidermal water loss, and promoting hair follicle health.

[0011] Preferably, ergothioneine is in the fermentation process, and the fermentation microorganism is Saccharomyces and Aspergillus optimized by gene screening, and the fermentation conditions are accurately controlled to be 28 DEG C -32 DEG C of temperature, 5.5-7.5 of pH value, and sterile filtered air is continuously passed through the fermentation process, and the ventilation volume is 0.5-1.5vvm, and the fermentation time is 48-72 hours, and after fermentation is completed, multi-stage membrane filtration and ion exchange resin purification process are adopted to purify.

[0012] Preferably, a specific complex enzyme is used in the process of extracting and enzymolyzing the collagen tripeptide, the complex enzyme includes papain, bromelain and neutral protease, the ratio of the three is 2:1:1, the enzymolysis time is 3-5 hours, and the enzymolysis temperature is 45°C-55°C;

[0013] After enzymatic hydrolysis, the product is purified by ultrafiltration using an ultrafiltration membrane with a molecular weight cutoff of 1000Da, and further refined by ion exchange chromatography and gel filtration chromatography to remove impurities and large molecular proteins and improve product purity. The moisture content of the final product is less than 5% after drying.

[0014] Preferably, the biological enzymes used in the enzymatic hydrolysis process of marine fish oligopeptides include alkaline protease and trypsin, the enzymatic hydrolysis time is 4-6 hours, and the enzymatic hydrolysis temperature is 40°C-50°C;

[0015] During the pretreatment process, the cod fish skin is firstly washed with alkali to remove surface impurities, the alkali solution is a sodium hydroxide solution with a concentration of 0.5%-1.5%, and the treatment time is 30-60 minutes;

[0016] Subsequently, an organic solvent extraction method is used to remove the grease. The organic solvent selected is food-grade n-hexane. The extraction temperature is 30°C-40°C and the extraction time is 1-2 hours. After extraction, vacuum drying is performed to remove residual solvent to ensure product quality and safety.

[0017] Preferably, the packaging material is a food-grade composite material with good moisture-proof and oxygen-isolating properties;

[0018] The packaging process is carried out in a sterile purification workshop with a cleanliness level of more than 100,000. The packaging environment temperature is 18℃-26℃ and the relative humidity is 30%-50%.

[0019] After packaging, the product needs to undergo accelerated stability testing and be placed at a temperature of 37°C ± 2°C and a relative humidity of 75% ± 5% for 3-6 months. The active ingredient content, microbial indicators, etc. must be regularly tested to ensure that the product quality is stable within the specified shelf life.

[0020] A method for preparing an anti-aging, beauty and skin care health food comprises the following steps:

[0021] S1. Raw material preparation;

[0022] S1.1, preparation of ergothioneine:

[0023] Selecting a Basidiomycetes edible fungus strain capable of producing ergothioneine for fermentation culture;

[0024] The fermentation medium contains ingredients such as carbon source, nitrogen source, and inorganic salts;

[0025] The carbon source is selected from glucose, sucrose or a combination thereof, with a concentration of 10-30 g / L;

[0026] The nitrogen source is yeast powder, peptone or a combination thereof, with a concentration of 5-20 g / L;

[0027] Inorganic salts include magnesium sulfate, potassium dihydrogen phosphate, etc., with a concentration of 0.5-5g / L;

[0028] The fermentation conditions are 28°C-32°C, pH 5.5-7.5, continuous introduction of sterile filtered air during the fermentation process, ventilation volume of 0.5-1.5 vvm, and fermentation time of 48-72 hours;

[0029] After fermentation is completed, multi-stage membrane filtration is used to remove bacteria and macromolecular impurities, and then purified by ion exchange resin purification process to obtain high-purity ergothioneine;

[0030] S1.2, preparation of collagen tripeptide;

[0031] Select high-quality collagen source, pre-treat it, dissolve the collagen in a buffer solution, and then add a specific complex enzyme for enzymatic hydrolysis. The complex enzyme includes papain, bromelain and neutral protease, and the ratio of the three is 2:1:1;

[0032] During the enzymatic hydrolysis process, the temperature is strictly controlled at 45℃-55℃ and the time is 3-5 hours;

[0033] After the enzymatic hydrolysis is completed, the temperature is raised to 80-90°C to inactivate the enzyme for 10-20 minutes, followed by an ultrafiltration purification step, using an ultrafiltration membrane with a molecular weight cutoff of 1000Da, and further refined by ion exchange chromatography and gel filtration chromatography to remove impurities and macromolecular proteins, and finally dried to obtain collagen tripeptide with a water content of less than 5%;

[0034] S1.3, Preparation of marine fish oligopeptides;

[0035] Using the deep-sea pollution-free cod skin as raw material, firstly remove the surface impurities through alkali washing, the alkali solution is a sodium hydroxide solution with a concentration of 0.5%-1.5%, and the treatment time is 30-60 minutes;

[0036] Then, an organic solvent extraction method is used to remove the grease. The organic solvent is food-grade n-hexane. The extraction temperature is 30°C-40°C. The extraction time is 1-2 hours. After extraction, the residual solvent is removed by vacuum drying.

[0037] The treated fish skin is subjected to a unique biological enzymatic hydrolysis process, wherein the biological enzymes used for enzymatic hydrolysis include alkaline protease and trypsin, the enzymatic hydrolysis time is 4-6 hours, and the enzymatic hydrolysis temperature is 40℃-50℃;

[0038] After enzymatic hydrolysis, separation, purification, drying and other steps are performed to obtain marine fish oligopeptides with an average molecular weight of less than 1000Da, wherein the content of oligopeptides with a molecular weight below 500Da is not less than 80%;

[0039] S2, mixed;

[0040] Ergothioneine, collagen tripeptide and marine fish oligopeptide are mixed evenly according to a predetermined ratio under specific environmental conditions. The mixing equipment uses an advanced three-dimensional mixer to ensure that the mixing uniformity error does not exceed ±1%;

[0041] The predetermined ratio is ergothioneine: collagen tripeptide: marine fish oligopeptide = 1:5:10 to 1:20:30, the temperature of the mixing process is strictly controlled at 20°C to 25°C, and the relative humidity is maintained in an environment not exceeding 40% to ensure the stability of the active ingredients;

[0042] S3, molding;

[0043] Accurately select appropriate excipients and molding processes according to the dosage form requirements of health foods;

[0044] The mixture is freeze-dried in vacuum, crushed and sieved after drying, with the mesh size of 80 to 120 meshes, and then mixed evenly, and processed into tablets or filled into capsules using advanced tableting equipment or capsule filling equipment;

[0045] S4, inspection;

[0046] Conduct multi-dimensional quality inspections on manufactured health foods to ensure that the content of each active ingredient meets precise requirements and meets hygiene standards;

[0047] Quality inspection covers many aspects such as microbiological testing, heavy metal testing, and active ingredient content testing;

[0048] In microbial testing, the total colony count shall not exceed 1000 CFU / g, the total number of molds and yeasts shall not exceed 100 CFU / g, and no pathogenic bacteria shall be detected; in heavy metal testing, the lead content shall not exceed 1.5 mg / kg, and the mercury content shall not exceed 0.3 mg / kg.

[0049] Preferably, the excipients include fillers, disintegrants, lubricants, sweeteners, and flavors;

[0050] The filler is selected from one or more combinations of microcrystalline cellulose, starch, and lactose, and the mass proportion of the filler in the auxiliary material is 30%-60%;

[0051] The disintegrant is selected from one or more combinations of cross-linked polyvinylpyrrolidone, sodium carboxymethyl starch, and low-substituted hydroxypropyl cellulose, and the mass proportion of the disintegrant in the auxiliary material is 10%-30%;

[0052] The lubricant is selected from one or more combinations of magnesium stearate, micro-powder silica gel, and talcum powder, and the mass proportion of the lubricant in the auxiliary material is 1%-5%;

[0053] The sweetener is selected from one or more combinations of sucrose, xylitol, and aspartame, and the weight proportion of the sweetener in the auxiliary material is 5%-20%;

[0054] The flavor is selected from natural fruit flavors or plant flavors, and the weight proportion of the flavors in the auxiliary materials is 0.1%-2%;

[0055] The quality of each auxiliary material meets the relevant food standards, and the amount added is determined according to the dosage form and taste requirements of the health food.

[0056] Preferably, during the preparation process, other active ingredients with anti-aging and beauty-enhancing effects, including vitamin C, vitamin E, and hyaluronic acid, may be added to further enhance the efficacy of the health food;

[0057] Vitamin C is L-ascorbic acid, and its added amount is 50 mg to 200 mg per 100 g of health food;

[0058] Vitamin E is natural vitamin E, and its added amount is 10 mg to 50 mg per 100 g of health food;

[0059] The molecular weight of hyaluronic acid is between 800,000 and 1.2 million, and the added amount is 10 mg to 30 mg per 100 g of health food;

[0060] When adding other active ingredients, a step-by-step mixing method is adopted. The vitamins are first pre-mixed with some auxiliary materials, then mixed with the main ingredients, and then hyaluronic acid is added and mixed again to ensure that the ingredients are evenly dispersed and do not react adversely with each other.

[0061] Preferably, during the quality inspection process, in addition to conventional microbial testing, heavy metal testing, and active ingredient content testing, structural identification and activity verification testing of the functional ingredients are also required;

[0062] Structural identification uses high performance liquid chromatography-mass spectrometry, nuclear magnetic resonance technology, etc.

[0063] Activity verification tests include in vitro cell experiments and animal experiments. In vitro cell experiments use human skin fibroblasts to observe the effects of the product on cell proliferation and collagen synthesis.

[0064] The animal experiment uses mice as the model, and by feeding the product to mice, the changes in indicators such as skin moisture content and hair health are observed to ensure that the product has clear anti-aging and beauty-enhancing effects.

[0065] Compared with the prior art, the present invention has the following beneficial effects:

[0066] The present invention overcomes the problems of low purity, many impurities and unstable sources of existing ergothioneine products by optimizing fermentation and purification processes, so that the purity of ergothioneine reaches more than 95%, and synergizes with collagen tripeptide and marine fish oligopeptide to fully regulate the relevant mechanism of human collagen, and jointly exerts anti-aging and beauty-maintaining effects from the cell level to the skin tissue level. The precise molecular weight control and high GPH content of collagen tripeptide ensure its efficient stimulation effect on skin collagen synthesis, and compared with existing collagen products, the absorption utilization rate is higher and the effect is more significant. The high purity and specific molecular weight distribution of marine fish oligopeptide enable it to have better stability and absorbability, and can better exert the regulating effect, and solve the problem that the existing ergothioneine has limited sources and low purity, and it is difficult to fully exert its anti-aging and beauty-maintaining effects. DETAILED DESCRIPTION

[0067] In order to facilitate those skilled in the art to understand the technical solution of the present invention, the technical solution of the present invention is further explained in conjunction with the specification.

[0068] An anti-aging and beauty health food, comprising thioneine as a core active ingredient, wherein the source of thioneine includes natural edible fungi, microbial fermentation products or a combination thereof, and the purity of thioneine reaches more than 95% after extraction or fermentation and purification process, and the food has high-efficiency antioxidant capacity, can effectively remove various free radicals in the body, and protect the integrity of cell structure and function, and the effective amount of thioneine in every 100 grams of the health food is 5 mg to 50 mg;

[0069] With collagen tripeptide and marine fish oligopeptide as auxiliary active ingredients, collagen tripeptide contains a specific high proportion of glycine-proline-hydroxyproline (GPH) structure, extracted from high-quality collagen source through targeted enzymatic hydrolysis technology, with its molecular weight precisely controlled between 279.3 and 294.4, and the GPH content is not less than 3.0%;

[0070] Marine fish oligopeptides are made from deep-sea pollution-free cod skin and refined through a unique bio-enzymatic hydrolysis process. They are highly stable and easy to absorb, with an average molecular weight of less than 1000Da, and the content of oligopeptides with a molecular weight below 500Da is not less than 80%;

[0071] The three ingredients work synergistically to achieve anti-aging and beauty-enhancing effects by regulating the synthesis and distribution of collagen in the human body, increasing skin moisture content, reducing transepidermal water loss, and promoting hair follicle health.

[0072] In an embodiment of the present invention, ergothioneine is in the fermentation process, and fermentation microorganism is Saccharomyces and Aspergillus optimized by gene screening, and fermentation condition is accurately controlled to be temperature 28 DEG C-32 DEG C, pH value 5.5-7.5, and sterile filtered air is continuously introduced in the fermentation process, and ventilation volume is 0.5-1.5vvm, and fermentation time is 48-72 hours, and multi-stage membrane filtration and ion exchange resin purification process are adopted to purify after fermentation is completed, and collagen tripeptide adopts specific composite enzyme in extraction enzymolysis process, and the composite enzyme includes papain, bromelain and neutral protease, and the ratio of the three is 2:1:1, and enzymolysis time is 3-5 hours, and enzymolysis temperature is 45 DEG C-55 DEG C, and after enzymolysis, ultrafiltration purification step is passed, and ultrafiltration with molecular weight cut-off of 1000Da is adopted. The membrane is further refined by ion exchange chromatography and gel filtration chromatography to remove impurities and macromolecular proteins and improve product purity. The moisture content of the final product is less than 5% after drying. The biological enzymes used in the enzymatic hydrolysis process of marine fish oligopeptides include alkaline protease and trypsin. The enzymatic hydrolysis time is 4-6 hours and the enzymatic hydrolysis temperature is 40°C-50°C. During the pretreatment process of cod skin, surface impurities are first removed by alkali washing. The alkali solution is a sodium hydroxide solution with a concentration of 0.5%-1.5%. The treatment time is 30-60 minutes. Subsequently, an organic solvent extraction method is used to remove grease. The organic solvent is food-grade n-hexane. The extraction temperature is 30°C-40°C and the extraction time is 1-2 hours. After extraction, the residual solvent is removed by vacuum drying to ensure product quality and safety.

[0073] In the embodiment of the present invention, the packaging material is a food-grade composite material having good moisture-proof and oxygen-isolating properties;

[0074] The packaging process is carried out in a sterile purification workshop with a cleanliness level of more than 100,000. The packaging environment temperature is 18℃-26℃ and the relative humidity is 30%-50%.

[0075] After packaging, the product needs to undergo accelerated stability testing and be placed at a temperature of 37°C ± 2°C and a relative humidity of 75% ± 5% for 3-6 months. The active ingredient content, microbial indicators, etc. must be regularly tested to ensure that the product quality is stable within the specified shelf life.

[0076] A method for preparing an anti-aging, beauty and skin care health food comprises the following steps:

[0077] S1. Raw material preparation;

[0078] S1.1, preparation of ergothioneine:

[0079] Selecting a Basidiomycetes edible fungus strain capable of producing ergothioneine for fermentation culture;

[0080] The fermentation medium contains ingredients such as carbon source, nitrogen source, and inorganic salts;

[0081] The carbon source is selected from glucose, sucrose or a combination thereof, with a concentration of 10-30 g / L;

[0082] The nitrogen source is yeast powder, peptone or a combination thereof, with a concentration of 5-20 g / L;

[0083] Inorganic salts include magnesium sulfate, potassium dihydrogen phosphate, etc., with a concentration of 0.5-5g / L;

[0084] The fermentation conditions are 28°C-32°C, pH 5.5-7.5, continuous introduction of sterile filtered air during the fermentation process, ventilation volume of 0.5-1.5 vvm, and fermentation time of 48-72 hours;

[0085] After fermentation is completed, multi-stage membrane filtration is used to remove bacteria and macromolecular impurities, and then purified by ion exchange resin purification process to obtain high-purity ergothioneine;

[0086] S1.2, preparation of collagen tripeptide;

[0087] Select high-quality collagen source, pre-treat it, dissolve the collagen in a buffer solution, and then add a specific complex enzyme for enzymatic hydrolysis. The complex enzyme includes papain, bromelain and neutral protease, and the ratio of the three is 2:1:1;

[0088] During the enzymatic hydrolysis process, the temperature is strictly controlled at 45℃-55℃ and the time is 3-5 hours;

[0089] After the enzymatic hydrolysis is completed, the temperature is raised to 80-90°C to inactivate the enzyme for 10-20 minutes, followed by an ultrafiltration purification step, using an ultrafiltration membrane with a molecular weight cutoff of 1000Da, and further refined by ion exchange chromatography and gel filtration chromatography to remove impurities and macromolecular proteins, and finally dried to obtain collagen tripeptide with a water content of less than 5%;

[0090] S1.3, Preparation of marine fish oligopeptides;

[0091] Using the deep-sea pollution-free cod skin as raw material, firstly remove the surface impurities through alkali washing, the alkali solution is a sodium hydroxide solution with a concentration of 0.5%-1.5%, and the treatment time is 30-60 minutes;

[0092] Then, an organic solvent extraction method is used to remove the grease. The organic solvent is food-grade n-hexane. The extraction temperature is 30°C-40°C. The extraction time is 1-2 hours. After extraction, the residual solvent is removed by vacuum drying.

[0093] The treated fish skin is subjected to a unique biological enzymatic hydrolysis process, wherein the biological enzymes used for enzymatic hydrolysis include alkaline protease and trypsin, the enzymatic hydrolysis time is 4-6 hours, and the enzymatic hydrolysis temperature is 40℃-50℃;

[0094] After enzymatic hydrolysis, separation, purification, drying and other steps are performed to obtain marine fish oligopeptides with an average molecular weight of less than 1000Da, wherein the content of oligopeptides with a molecular weight below 500Da is not less than 80%;

[0095] S2, mixed;

[0096] Ergothioneine, collagen tripeptide and marine fish oligopeptide are mixed evenly according to a predetermined ratio under specific environmental conditions. The mixing equipment uses an advanced three-dimensional mixer to ensure that the mixing uniformity error does not exceed ±1%;

[0097] The predetermined ratio is ergothioneine: collagen tripeptide: marine fish oligopeptide = 1:5:10 to 1:20:30, the temperature of the mixing process is strictly controlled at 20°C to 25°C, and the relative humidity is maintained in an environment not exceeding 40% to ensure the stability of the active ingredients;

[0098] S3, molding;

[0099] Accurately select appropriate excipients and molding processes according to the dosage form requirements of health foods;

[0100] The mixture is freeze-dried in vacuum, crushed and sieved after drying, with the mesh size of 80 to 120 meshes, and then mixed evenly, and processed into tablets or filled into capsules using advanced tableting equipment or capsule filling equipment;

[0101] S4, inspection;

[0102] Conduct multi-dimensional quality inspections on manufactured health foods to ensure that the content of each active ingredient meets precise requirements and meets hygiene standards;

[0103] Quality inspection covers many aspects such as microbiological testing, heavy metal testing, and active ingredient content testing;

[0104] In microbial testing, the total colony count shall not exceed 1000 CFU / g, the total number of molds and yeasts shall not exceed 100 CFU / g, and no pathogenic bacteria shall be detected; in heavy metal testing, the lead content shall not exceed 1.5 mg / kg, and the mercury content shall not exceed 0.3 mg / kg.

[0105] In an embodiment of the present invention, the auxiliary materials include fillers, disintegrants, lubricants, sweeteners, and flavors;

[0106] The filler is selected from one or more combinations of microcrystalline cellulose, starch, and lactose, and the mass proportion of the filler in the auxiliary material is 30%-60%;

[0107] The disintegrant is selected from one or more combinations of cross-linked polyvinylpyrrolidone, sodium carboxymethyl starch, and low-substituted hydroxypropyl cellulose, and the mass proportion of the disintegrant in the auxiliary material is 10%-30%;

[0108] The lubricant is selected from one or more combinations of magnesium stearate, micro-powder silica gel, and talcum powder, and the mass proportion of the lubricant in the auxiliary material is 1%-5%;

[0109] The sweetener is selected from one or more combinations of sucrose, xylitol, and aspartame, and the weight proportion of the sweetener in the auxiliary material is 5%-20%;

[0110] The flavor is selected from natural fruit flavors or plant flavors, and the weight proportion of the flavors in the auxiliary materials is 0.1%-2%;

[0111] The quality of each auxiliary material meets the relevant food standards, and the amount added is determined according to the dosage form and taste requirements of the health food.

[0112] In an embodiment of the present invention, other active ingredients with anti-aging and beauty-enhancing effects, including vitamin C, vitamin E, and hyaluronic acid, can be added during the preparation process to further enhance the efficacy of the health food. Vitamin C is L-ascorbic acid, and its addition amount is 50 mg to 200 mg per 100 g of health food. Vitamin E is natural vitamin E, and its addition amount is 10 mg to 50 mg per 100 g of health food. The molecular weight of hyaluronic acid is between 800,000 and 1.2 million, and its addition amount is 10 mg to 30 mg per 100 g of health food. When adding other active ingredients, a step-by-step mixing method is adopted, wherein the vitamins are pre-mixed with some auxiliary materials, and then mixed with the main ingredients. After mixing, hyaluronic acid is added and mixed again to ensure that the ingredients are evenly dispersed and there are no adverse reactions to each other. During the quality inspection process, in addition to the conventional microbial testing, heavy metal testing, and active ingredient content testing, structural identification and activity verification tests of the effective ingredients are also required. The structural identification adopts high-performance liquid chromatography-mass spectrometry, nuclear magnetic resonance technology, etc. The activity verification test includes in vitro cell experiments and animal experiments. The in vitro cell experiment uses human skin fibroblasts to observe the effect of the product on cell proliferation and collagen synthesis. The animal experiment uses a mouse model to observe changes in indicators such as skin moisture content and hair health by feeding the product to ensure that the product has clear anti-aging and beauty-enhancing effects.

[0113] The embodiments of the present invention disclose preferred embodiments, but are not limited thereto. A person skilled in the art can easily understand the spirit of the present invention based on the above embodiments and make different extensions and changes. However, as long as they do not deviate from the spirit of the present invention, they are all within the protection scope of the present invention.

Claims

1. An anti-aging, beauty and skin care food, characterized in that: The invention comprises thioneine as the core active ingredient, the source of thioneine includes natural edible fungi, microbial fermentation products or a combination thereof, and the purity of thioneine reaches more than 95% after extraction or fermentation and purification process, and has high-efficiency antioxidant capacity, can effectively remove various free radicals in the body, and protect the integrity of cell structure and function. The effective amount of thioneine in every 100 grams of health food is 5 mg to 50 mg; With collagen tripeptide and marine fish oligopeptide as auxiliary active ingredients, collagen tripeptide contains a specific high proportion of glycine-proline-hydroxyproline (GPH) structure, extracted from high-quality collagen source through targeted enzymatic hydrolysis technology, with its molecular weight precisely controlled between 279.3 and 294.4, and the GPH content is not less than 3.0%; Marine fish oligopeptides are made from deep-sea pollution-free cod skin and refined through a unique bio-enzymatic hydrolysis process. They are highly stable and easy to absorb, with an average molecular weight of less than 1000Da, and the content of oligopeptides with a molecular weight below 500Da is not less than 80%; The three ingredients work synergistically to achieve anti-aging and beauty-enhancing effects by regulating the synthesis and distribution of collagen in the human body, increasing skin moisture content, reducing transepidermal water loss, and promoting hair follicle health.

2. The anti-aging, beauty and skin care food according to claim 1, characterized in that: During the fermentation process of ergothioneine, the fermentation microorganisms are yeast and Aspergillus that have been optimized through genetic screening. The fermentation conditions are precisely controlled at a temperature of 28°C-32°C and a pH value of 5.5-7.

5. Sterile filtered air is continuously introduced during the fermentation process with a ventilation volume of 0.5-1.5vvm. The fermentation time is 48-72 hours. After the fermentation is completed, multi-stage membrane filtration and ion exchange resin purification process are used for purification.

3. The anti-aging, beauty and skin care food according to claim 2, characterized in that: Collagen tripeptide uses a specific complex enzyme in the process of extraction and enzymatic hydrolysis. The complex enzyme includes papain, bromelain and neutral protease. The ratio of the three is 2:1:

1. The enzymatic hydrolysis time is 3-5 hours and the enzymatic hydrolysis temperature is 45℃-55℃. After enzymatic hydrolysis, the product is purified by ultrafiltration using an ultrafiltration membrane with a molecular weight cutoff of 1000Da, and further refined by ion exchange chromatography and gel filtration chromatography to remove impurities and large molecular proteins and improve product purity. The moisture content of the final product is less than 5% after drying.

4. The anti-aging, beauty and skin care food according to claim 3, characterized in that: The biological enzymes used in the enzymatic hydrolysis process of marine fish oligopeptides include alkaline protease and trypsin, the enzymatic hydrolysis time is 4-6 hours, and the enzymatic hydrolysis temperature is 40℃-50℃; During the pretreatment process, the cod fish skin is firstly washed with alkali to remove surface impurities, the alkali solution is a sodium hydroxide solution with a concentration of 0.5%-1.5%, and the treatment time is 30-60 minutes; Subsequently, an organic solvent extraction method is used to remove the grease. The organic solvent selected is food-grade n-hexane. The extraction temperature is 30°C-40°C and the extraction time is 1-2 hours. After extraction, vacuum drying is performed to remove residual solvent to ensure product quality and safety.

5. The anti-aging, beauty and skin care food according to claim 4, characterized in that: Its packaging material is made of food-grade composite material, which has good moisture-proof and oxygen-isolating properties; The packaging process is carried out in a sterile purification workshop with a cleanliness level of more than 100,000. The packaging environment temperature is 18℃-26℃ and the relative humidity is 30%-50%. After packaging, the product needs to undergo accelerated stability testing and be placed at a temperature of 37°C ± 2°C and a relative humidity of 75% ± 5% for 3-6 months. The active ingredient content, microbial indicators, etc. must be regularly tested to ensure that the product quality is stable within the specified shelf life.

6. A method for preparing an anti-aging, beauty and skin care food, characterized in that: The following steps are involved: S1. Raw material preparation; S1.1, preparation of ergothioneine: Selecting a Basidiomycetes edible fungus strain capable of producing ergothioneine for fermentation culture; The fermentation medium contains ingredients such as carbon source, nitrogen source, and inorganic salts; The carbon source is selected from glucose, sucrose or a combination thereof, with a concentration of 10-30 g / L; The nitrogen source is yeast powder, peptone or a combination thereof, with a concentration of 5-20 g / L; Inorganic salts include magnesium sulfate, potassium dihydrogen phosphate, etc., with a concentration of 0.5-5g / L; The fermentation conditions are 28°C-32°C, pH 5.5-7.5, continuous introduction of sterile filtered air during the fermentation process, ventilation volume of 0.5-1.5 vvm, and fermentation time of 48-72 hours; After fermentation is completed, multi-stage membrane filtration is used to remove bacteria and macromolecular impurities, and then purified by ion exchange resin purification process to obtain high-purity ergothioneine; S1.2, preparation of collagen tripeptide; Select high-quality collagen source, pre-treat it, dissolve the collagen in a buffer solution, and then add a specific complex enzyme for enzymatic hydrolysis. The complex enzyme includes papain, bromelain and neutral protease, and the ratio of the three is 2:1:1; During the enzymatic hydrolysis process, the temperature is strictly controlled at 45℃-55℃ and the time is 3-5 hours; After the enzymatic hydrolysis is completed, the temperature is raised to 80-90°C to inactivate the enzyme for 10-20 minutes, followed by an ultrafiltration purification step, using an ultrafiltration membrane with a molecular weight cutoff of 1000Da, and further refined by ion exchange chromatography and gel filtration chromatography to remove impurities and macromolecular proteins, and finally dried to obtain collagen tripeptide with a water content of less than 5%; S1.3, Preparation of marine fish oligopeptides; Using the deep-sea pollution-free cod skin as raw material, firstly remove the surface impurities through alkali washing, the alkali solution is a sodium hydroxide solution with a concentration of 0.5%-1.5%, and the treatment time is 30-60 minutes; Then, an organic solvent extraction method is used to remove the grease. The organic solvent is food-grade n-hexane. The extraction temperature is 30°C-40°C and the extraction time is 1-2 hours. After extraction, the residual solvent is removed by vacuum drying. The treated fish skin is subjected to a unique biological enzymatic hydrolysis process, wherein the biological enzymes used for enzymatic hydrolysis include alkaline protease and trypsin, the enzymatic hydrolysis time is 4-6 hours, and the enzymatic hydrolysis temperature is 40℃-50℃; After enzymatic hydrolysis, separation, purification, drying and other steps are performed to obtain marine fish oligopeptides with an average molecular weight of less than 1000Da, wherein the content of oligopeptides with a molecular weight below 500Da is not less than 80%; S2, mixed; Ergothioneine, collagen tripeptide and marine fish oligopeptide are mixed evenly according to a predetermined ratio under specific environmental conditions. The mixing equipment uses an advanced three-dimensional mixer to ensure that the mixing uniformity error does not exceed ±1%; The predetermined ratio is ergothioneine: collagen tripeptide: marine fish oligopeptide = 1:5:10 to 1:20:30, the temperature of the mixing process is strictly controlled at 20°C to 25°C, and the relative humidity is maintained in an environment not exceeding 40% to ensure the stability of the active ingredients; S3, molding; Accurately select appropriate excipients and molding processes according to the dosage form requirements of health foods; The mixture is freeze-dried in vacuum, crushed and sieved after drying, with the mesh size of 80 to 120 meshes, and then mixed evenly, and processed into tablets or filled into capsules using advanced tableting equipment or capsule filling equipment; S4, inspection; Conduct multi-dimensional quality inspections on manufactured health foods to ensure that the content of each active ingredient meets precise requirements and meets hygiene standards; Quality inspection covers many aspects such as microbiological testing, heavy metal testing, and active ingredient content testing; In microbial testing, the total colony count shall not exceed 1000 CFU / g, the total number of molds and yeasts shall not exceed 100 CFU / g, and no pathogenic bacteria shall be detected; in heavy metal testing, the lead content shall not exceed 1.5 mg / kg, and the mercury content shall not exceed 0.3 mg / kg.

7. The method for preparing an anti-aging, beauty and skin care food according to claim 6, characterized in that: Excipients include fillers, disintegrants, lubricants, sweeteners, and flavors; The filler is selected from one or more combinations of microcrystalline cellulose, starch, and lactose, and the mass proportion of the filler in the auxiliary material is 30%-60%; The disintegrant is selected from one or more combinations of cross-linked polyvinylpyrrolidone, sodium carboxymethyl starch, and low-substituted hydroxypropyl cellulose, and the mass proportion of the disintegrant in the auxiliary material is 10%-30%; The lubricant is selected from one or more combinations of magnesium stearate, micro-powder silica gel, and talcum powder, and the mass proportion of the lubricant in the auxiliary material is 1%-5%; The sweetener is selected from one or more combinations of sucrose, xylitol, and aspartame, and the weight proportion of the sweetener in the auxiliary material is 5%-20%; The flavor is selected from natural fruit flavors or plant flavors, and the weight proportion of the flavors in the auxiliary materials is 0.1%-2%; The quality of each auxiliary material meets the relevant food standards, and the amount added is determined according to the dosage form and taste requirements of the health food.

8. The method for preparing an anti-aging, beauty and skin care food according to claim 7, characterized in that: During the preparation process, other active ingredients with anti-aging and beauty-enhancing effects, including vitamin C, vitamin E, and hyaluronic acid, can also be added to further enhance the efficacy of the health food; Vitamin C is L-ascorbic acid, and its added amount is 50 mg to 200 mg per 100 g of health food; Vitamin E is natural vitamin E, and its added amount is 10 mg to 50 mg per 100 g of health food; The molecular weight of hyaluronic acid is between 800,000 and 1.2 million, and the added amount is 10 mg to 30 mg per 100 g of health food; When adding other active ingredients, a step-by-step mixing method is adopted. The vitamins are first pre-mixed with some auxiliary materials, then mixed with the main ingredients, and then hyaluronic acid is added and mixed again to ensure that the ingredients are evenly dispersed and do not react adversely with each other.

9. The method for preparing an anti-aging, beauty and skin care food according to claim 8, characterized in that: During the quality inspection process, in addition to conventional microbial testing, heavy metal testing, and active ingredient content testing, structural identification of the active ingredients and activity verification tests are also required; Structural identification uses high performance liquid chromatography-mass spectrometry, nuclear magnetic resonance technology, etc. Activity verification tests include in vitro cell experiments and animal experiments. In vitro cell experiments use human skin fibroblasts to observe the effects of the product on cell proliferation and collagen synthesis. The animal experiment uses mice as the model, and by feeding the product to mice, the changes in indicators such as skin moisture content and hair health are observed to ensure that the product has clear anti-aging and beauty-enhancing effects.