Rice fermentation product filtrate and application of ectoine in inhibiting and / or reducing sebum production

Abstract

The invention provides application of rice fermentation product filtrate and Ectoine in inhibiting and / or reducing sebum production, wherein the sebum production is sebum production caused by fatty acids and hormones. The application finds that rice fermentation product filtrate or Ectoin has inhibiting and / or reducing effects on sebum production caused by various factors, such as fatty acids and hormones. Furthermore, the rice fermentation product filtrate and Ectoin are combined for use, so that a synergistic inhibition and / or reduction effect can be achieved on sebum production caused by various factors such as fatty acid and hormone.

Description

Technical Field

[0001] The present application belongs to the field of cosmetic technology, and specifically, relates to the use of rice fermentation product filtrate and ectoine in inhibiting and / or reducing sebum production. Background Art

[0002] Studies have confirmed that sebocyte proliferation, differentiation and adipogenesis are affected by pathways activated by ligand-receptor complexes, such as hormones: androgens, estrogens, cortisone, dihydrotestosterone (DHT), glucocorticoids, adrenal androgens, etc. In addition, essential fatty acids linoleic acid and arachidonic acid are natural ligands of peroxisome proliferator-activated receptors (PPAR), which strongly promote the accumulation of lipid droplets in the cytoplasm, thereby increasing oil secretion.

[0003] If the sebum secretion is too much, the skin will become dirty easily, which will stimulate the sebum secretion and cause acne. In severe cases, symptoms such as acne and seborrheic dermatitis may also appear. Common acne is a common inflammatory skin disease of the hair follicles and sebaceous glands during adolescence, commonly known as acne. The pathological changes are mainly due to the vigorous secretion and metabolism of male hormones, more sebum secretion, and the invasion of Propionibacterium acnes in the hair follicles, often accompanied by secondary inflammatory lesions. The early lesions are blackheads located at the opening of the hair follicles, which later develop into inflammatory papules, pustules, nodules or abscesses.

[0004] The fast pace of daily life, irregular day and night, and high stress of modern people all lead to increased sebum secretion. The oxidation of sebum can cause dull skin. The increased sebum can cause acne, pimples, acne, blackheads, inflammatory papules, pustules, nodules, abscesses, dandruff, oily skin, rough skin and other problems. Therefore, how to reduce / inhibit the production of sebum to make the skin healthier and more beautiful is still the goal pursued in the cosmetics field. Summary of the invention

[0005] After long-term research, the inventors of the present application found that rice fermentation product filtrate and / or ectoine have the effect of inhibiting and / or reducing the production of oil caused by fatty acids and hormones, so that the above-mentioned substances can be used in the care of oily skin that is easily affected by hormones and fatty acids.

[0006] Specifically, this application involves the following aspects:

[0007] 1. Use of rice fermentation product filtrate and ectoine in inhibiting and / or reducing sebum production, wherein the sebum production is sebum production caused by fatty acids and hormones;

[0008] Preferably, the fatty acid comprises arachidonic acid and / or linoleic acid;

[0009] Preferably, the hormone includes one or more of cortisone, 5α-dihydrotestosterone, androgen, estrogen, glucocorticoid, and adrenal androgen.

[0010] 2. The use according to item 1, wherein the mass ratio of the rice fermentation product filtrate to the ectoine is not less than 1;

[0011] Preferably, the mass ratio of the rice fermentation product filtrate to the ectoine is (1-200000):1, preferably (200-5000):1, and more preferably (200-1000):1.

[0012] 3. The use according to item 1 or 2, wherein the rice fermentation product filtrate comprises yeast / rice fermentation product filtrate.

[0013] 4. The use according to any one of items 1 to 3, wherein the rice fermentation product filtrate and ectoine are used to prevent and / or relieve excessive sebum secretion;

[0014] Preferably, the rice fermentation product filtrate and ectoine are used to prevent and / or alleviate the formation of one or more of acne, pimples, acne, blackheads, inflammatory papules, pustules, enlarged pores, dull skin color, nodules, abscesses, and dandruff.

[0015] 5. The use according to any one of items 1 to 4, wherein the rice fermentation product filtrate and ectoine are used to prepare an oil control active agent.

[0016] 6. The use according to any one of items 1 to 4, wherein the rice fermentation product filtrate and ectoine are used as oil control active agents.

[0017] 7. Use of rice fermentation product filtrate in inhibiting and / or reducing sebum production, wherein the sebum production is sebum production caused by fatty acids and hormones;

[0018] Preferably, the rice fermentation product filtrate comprises yeast / rice fermentation product filtrate;

[0019] Preferably, the fatty acid comprises arachidonic acid and / or linoleic acid;

[0020] Preferably, the hormone includes one or more of cortisone, 5α-dihydrotestosterone, androgen, estrogen, glucocorticoid, and adrenal androgen.

[0021] 8. The use according to item 7, wherein the rice fermentation product filtrate is used for preventing and / or alleviating excessive sebum secretion;

[0022] Preferably, the rice fermentation product filtrate is used to prevent and / or alleviate the formation of one or more of acne, pimples, acne, blackheads, inflammatory papules, pustules, enlarged pores, dull skin color, nodules, abscesses, and dandruff.

[0023] 9. The use according to item 7 or 8, wherein the filtrate of the rice fermentation product is used to prepare an oil-controlling active agent.

[0024] 10. The use according to item 7 or 8, wherein the filtrate of the rice fermentation product is used as an oil control active agent.

[0025] Beneficial Effects

[0026] The present application has found that rice fermentation product filtrate or ectoine has an inhibitory and / or reducing effect on sebum production caused by various factors, such as fatty acids and hormones.

[0027] Furthermore, the combined use of rice fermentation product filtrate and ectoine can have a synergistic inhibitory and / or reducing effect on sebum production caused by various factors, such as fatty acids and hormones.

[0028] Based on the above findings, the rice fermentation product filtrate and the combination of rice fermentation product filtrate and ectoine of the present application can be applied to a variety of oil control products, for example, oil control products for the increased sebum secretion easily caused by hormones and fatty acids. DETAILED DESCRIPTION

[0029] The present application is further described below in conjunction with examples. It should be understood that the examples are only used to further describe and illustrate the present application and are not used to limit the present application.

[0030] Unless otherwise defined, the technical and scientific terms in this specification have the same meaning as those generally understood by those skilled in the art. Although similar or identical methods and materials as described herein may be used in experiments or practical applications, materials and methods are described herein below. In the event of a conflict, the present specification including the definitions therein shall prevail. In addition, materials, methods and examples are provided for illustration only and are not restrictive. The present application is further described below in conjunction with specific embodiments, but is not intended to limit the scope of the present application.

[0031] In view of the problems existing in the prior art, the present application provides rice fermentation product filtrate, the use of rice fermentation product filtrate and ectoine in inhibiting and / or reducing sebum production, wherein the sebum production is sebum production caused by fatty acids and / or hormones.

[0032] Among them, the uses of rice fermentation product filtrate, rice fermentation product filtrate and ectoine in inhibiting and / or reducing sebum production include the use of rice fermentation product filtrate in inhibiting and / or reducing sebum production, and the use of a combination of rice fermentation product filtrate and ectoine in inhibiting and / or reducing sebum production.

[0033] The composition of sebum is relatively complex, mainly containing waxes, triglycerides, free fatty acids, and phospholipids and cholesterol produced by epithelial cell metabolism. Sebum is oily and moisturizes the skin to prevent dryness and cracking. The fatty acids in sebum can kill bacteria and resist mold and viruses, and can be called the natural barrier of the skin. Sebum protects and moisturizes the skin, but too much sebum will form seborrheic dermatitis, such as acne, pimples, acne, blackheads, inflammatory papules, pustules, nodules, abscesses, dandruff, oily skin, rough skin, etc.

[0034] The sebum content can be determined by any method known in the art, such as Nile red staining method and Oil red staining method.

[0035] The rice fermentation product filtrate of the present application refers to the fermentation filtrate obtained by removing impurities and sterilizing the fermentation liquid obtained by fermenting rice with bacteria.

[0036] In some specific embodiments, the rice fermentation product filtrate refers to the fermentation filtrate obtained by removing impurities and sterilizing the fermentation liquid obtained by fermenting rice with yeast, for example, including yeast / rice fermentation product filtrate.

[0037] The yeast / rice fermentation product filtrate can be prepared according to the method disclosed in the prior art, for example, according to the method disclosed in patents CN108324633B and CN112618464A, but these methods are not the only ones that can prepare brown rice fermentation filtrate.

[0038] The yeast / rice fermentation product filtrate can also be obtained through commercial channels, for example, it can be purchased from Bloomage Biotechnology Co., Ltd.

[0039] The fatty acids and hormones in the present application may include various fatty acids and hormones known in the art.

[0040] In some specific embodiments, the fatty acid comprises arachidonic acid.

[0041] In some specific embodiments, the fatty acid comprises linoleic acid.

[0042] In some specific embodiments, the fatty acids include arachidonic acid and linoleic acid.

[0043] In some specific embodiments, the fatty acid is arachidonic acid.

[0044] In some specific embodiments, the fatty acid is linoleic acid.

[0045] In some specific embodiments, the fatty acids are arachidonic acid and linoleic acid.

[0046] In some specific embodiments, the hormone includes one or more of cortisone, 5α-dihydrotestosterone, androgen, estrogen, glucocorticoid, and adrenal androgen.

[0047] In some specific embodiments, the hormone is one or more of cortisone, 5α-dihydrotestosterone, androgen, estrogen, glucocorticoid, and adrenal androgen.

[0048] The sebum production of the present application may be induced by fatty acids, hormones, or both fatty acids and hormones.

[0049] In some specific embodiments, sebum production is induced by arachidonic acid and linoleic acid.

[0050] In some specific embodiments, sebum production is induced by cortisone and dihydrotestosterone.

[0051] In some specific embodiments, sebum production is induced by linoleic acid and dihydrotestosterone.

[0052] In some specific embodiments, sebum production is induced by arachidonic acid and cortisone.

[0053] In some specific embodiments, the use is the use of rice fermentation product filtrate and ectoine in preventing and / or alleviating excessive sebum secretion.

[0054] It is known to those skilled in the art that excessive skin secretion may cause skin problems, such as excessive sebum secretion, which may cause acne, pimples, blackheads, inflammatory papules, pustules, enlarged pores, dull skin color, nodules, abscesses, dandruff, etc., or appearance defects such as oily skin and rough skin.

[0055] When the combination of rice fermentation product filtrate and ectoine is used together to inhibit and / or reduce sebum production, the mass ratio of rice fermentation product filtrate to ectoine is not less than 1 in some specific embodiments, for example, it can be 1:1, 2:1, 5:1, 10:1, 20:1, 30:1, 40:1, 50:1, 60:1, 70:1, 80:1, 90:1, 100:1, 150:1, 200:1, 250:1, 300:1, 350:1, 400:1, 450:1, 50 .... 00:1, 550:1, 600:1, 650:1, 700:1, 750:1, 800:1, 850:1, 900:1, 950:1, 1000:1, 1500:1, 2000:1, 2500:1, 3000:1, 3500:1, 4000:1, 4500:1, 5000:1, 10000:1, 20000:1, 50000:1, 100000:1, 200000:1, and any range therebetween.

[0056] In some specific embodiments, the mass ratio of rice fermentation product filtrate to ectoine is (1-200000):1.

[0057] In some specific embodiments, the mass ratio of rice fermentation product filtrate to ectoine is (200-5000):1.

[0058] In some specific embodiments, the mass ratio of rice fermentation product filtrate to ectoine is (200-1000):1.

[0059] In some specific embodiments, the present application provides the use of rice fermentation product filtrate and ectoine in inhibiting and / or reducing sebum production, wherein sebum production is caused by arachidonic acid and linoleic acid, and the mass ratio of rice fermentation product filtrate and ectoine is (1-200000):1.

[0060] In some specific embodiments, the present application provides the use of rice fermentation product filtrate and ectoine in inhibiting and / or reducing sebum production, wherein sebum production is caused by arachidonic acid and linoleic acid, and the mass ratio of rice fermentation product filtrate and ectoine is (200-5000):1.

[0061] In some specific embodiments, the present application provides the use of rice fermentation product filtrate and ectoine in inhibiting and / or reducing sebum production, wherein sebum production is caused by arachidonic acid and linoleic acid, and the mass ratio of rice fermentation product filtrate and ectoine is (200-1000):1.

[0062] In some specific embodiments, the present application provides the use of rice fermentation product filtrate and ectoine in inhibiting and / or reducing sebum production, wherein sebum production is caused by cortisone and dihydrotestosterone, and the mass ratio of rice fermentation product filtrate and ectoine is (1-200000):1.

[0063] In some specific embodiments, the present application provides the use of rice fermentation product filtrate and ectoine in inhibiting and / or reducing sebum production, wherein sebum production is caused by cortisone and dihydrotestosterone, and the mass ratio of rice fermentation product filtrate and ectoine is (200-5000):1.

[0064] In some specific embodiments, the present application provides the use of rice fermentation product filtrate and ectoine in inhibiting and / or reducing sebum production, wherein sebum production is caused by cortisone and dihydrotestosterone, and the mass ratio of rice fermentation product filtrate and ectoine is (200-1000):1.

[0065] In some specific embodiments, the present application provides the use of rice fermentation product filtrate and ectoine in inhibiting and / or reducing sebum production, wherein sebum production is caused by linoleic acid and dihydrotestosterone, and the mass ratio of rice fermentation product filtrate and ectoine is (1-200000):1.

[0066] In some specific embodiments, the present application provides the use of rice fermentation product filtrate and ectoine in inhibiting and / or reducing sebum production, wherein sebum production is caused by linoleic acid and dihydrotestosterone, and the mass ratio of rice fermentation product filtrate and ectoine is (200-5000):1.

[0067] In some specific embodiments, the present application provides the use of rice fermentation product filtrate and ectoine in inhibiting and / or reducing sebum production, wherein sebum production is caused by linoleic acid and dihydrotestosterone, and the mass ratio of rice fermentation product filtrate and ectoine is (200-1000):1.

[0068] In some specific embodiments, the present application provides the use of rice fermentation product filtrate and ectoine in inhibiting and / or reducing sebum production, wherein sebum production is caused by arachidonic acid and cortisone, and the mass ratio of rice fermentation product filtrate and ectoine is (1-200000):1.

[0069] In some specific embodiments, the present application provides the use of rice fermentation product filtrate and ectoine in inhibiting and / or reducing sebum production, wherein sebum production is caused by arachidonic acid and cortisone, and the mass ratio of rice fermentation product filtrate and ectoine is (200-5000):1.

[0070] In some specific embodiments, the present application provides the use of rice fermentation product filtrate and ectoine in inhibiting and / or reducing sebum production, wherein sebum production is caused by arachidonic acid and cortisone, and the mass ratio of rice fermentation product filtrate and ectoine is (200-1000):1.

[0071] The present application also provides the use of rice fermentation product filtrate and ectoine in the preparation of an oil-controlling active agent.

[0072] The present application also provides the use of rice fermentation product filtrate and ectoine as oil control active agents.

[0073] The present application also provides the use of the rice fermentation product filtrate in the preparation of an oil-controlling active agent.

[0074] The present application also provides the use of rice fermentation product filtrate as an oil control active agent.

[0075] The descriptions of rice fermentation product filtrate and ectoine in this application are as described above.

[0076] In summary, the present application finds that rice fermentation product filtrate or Ecdoin has an inhibitory and / or reducing effect on sebum production caused by fatty acids, hormones or a combination thereof. Especially when rice fermentation product filtrate and Ecdoin are used in combination, they can have a synergistic inhibitory and / or reducing effect on sebum production caused by fatty acids, hormones or a combination thereof.

[0077] Furthermore, the rice fermentation product filtrate and / or Ecdoin can be applied to a variety of oil control products to inhibit the production of sebum caused by fatty acids, hormones or a combination thereof. For example, these oil control products can be used in adolescent groups or groups with high stress to inhibit the increase in sebum secretion caused by hormones and fatty acids.

[0078] Example

[0079] The present application provides a general and / or specific description of the materials and test methods used in the experiments. In the following examples, unless otherwise specified, % means wt%, i.e., weight percentage. The reagents or instruments used without indicating the manufacturer are all conventional reagent products that can be obtained commercially.

[0080] Weigh yeast / rice fermentation product filtrate (purchased from Bloomage Biotechnology Co., Ltd., trade name: brown rice fermentation filtrate) and ectoine (purchased from Bloomage Biotechnology Co., Ltd.), mix and dissolve with SZ95 cell (human sebaceous gland cell) complete culture medium (containing 10% FBS, 1% penicillin-streptomycin) to obtain a composition containing brown rice fermentation filtrate and ectoine.

[0081] Among them, the mass ratio of ectoine and brown rice fermentation filtrate in the composition of Example 1 (T1) is 1:1.

[0082] The mass ratio of ectoine to brown rice fermentation filtrate in the composition of Example 2 (T2) is 1:5.

[0083] The mass ratio of ectoine to brown rice fermentation filtrate in the composition of Example 3 (T3) is 1:40.

[0084] The mass ratio of ectoine to brown rice fermentation filtrate in the composition of Example 4 (T4) is 1:500.

[0085] The mass ratio of ectoine to brown rice fermentation filtrate in the composition of Example 5 (T5) is 1:5000.

[0086] The mass ratio of ectoine to brown rice fermentation filtrate in the composition of Example 6 (T6) is 1:30000.

[0087] The mass ratio of ectoine to brown rice fermentation filtrate in the composition of Example 7 (T7) is 1:200000.

[0088] Example 8 only contains ectoine, and Example 9 only contains brown rice fermentation filtrate.

[0089] Specifically, the contents of each component in each embodiment are shown in Table 1.

[0090] Table 1

[0091]

[0092]

[0093] Experimental example

[0094] The compositions of the above examples were used as samples for the following experimental groups.

[0095] Experimental Example 1: Effects of brown rice fermentation filtrate and ectoin on lipid production under fatty acid stimulation (arachidonic acid (AA) and linoleic acid (LA))

[0096] Group settings:

[0097] Blank group: no AA+LA stimulation treatment, no sample treatment

[0098] Control group: AA+LA stimulation treatment, no sample treatment

[0099] Experimental group: AA+LA stimulation treatment, experimental group sample treatment

[0100] Test method: Take SZ95 cells and culture them in a T75 culture flask with DMEM medium. When the cell density is about 80%, inoculate them in a 24-well plate with a coverslip and culture them at 37°C and 5% CO2. After 24 hours, aspirate the supernatant, treat each well according to the above group settings, set 3 replicate wells for each group, and take 5 photos of each well randomly. Incubate in a 37°C, 5% CO2 incubator for 72 hours. Aspirate the supernatant, wash the cells twice, and add 4% paraformaldehyde to fix for 1 hour. After fixation, add 1μg / mL Nile red to each well and incubate in a 37°C, 5% CO2 incubator for 30 minutes, then observe and take photos under a fluorescence microscope, and finally use Image J software to semi-quantitatively analyze the fluorescence data of the photos.

[0101] According to the fluorescence data, the relative oil content of the blank group was set as 100%, and the data of other groups were normalized to obtain the relative oil content; the relative oil inhibition rate of the control group was set as 0%, and the data of other groups were normalized to obtain the relative oil inhibition rate. The t test was used for pairwise comparison, *P<0.05 indicated that the difference was statistically significant, **P<0.01 indicated that the difference was statistically significant, and ***P<0.001 indicated that the difference was extremely statistically significant.

[0102] The measurement results are shown in Table 2.

[0103] Table 2

[0104]

[0105] According to the analysis in Table 2, comparing the blank group and the control group, AA+LA treatment can significantly enhance the oil content in SZ95 cells. Examples 8 and 9 showed the efficacy of inhibiting AA+LA-induced oil production, while Examples 1-7 all effectively inhibited AA+LA-induced oil production to varying degrees, and their inhibitory effects were significantly different from those of Examples 8 and 9. Therefore, the brown rice fermentation filtrate and ectoine in the examples of the present application both have the efficacy of inhibiting fatty acid stimulation-induced oil production, and the combination has a stronger inhibitory effect on oil production than a single substance, and has a synergistic effect.

[0106] Experimental Example 2: Effects of brown rice fermentation filtrate and ectoine on lipid production under hormone stimulation (cortisone and dihydrotestosterone (DHT))

[0107] Group settings:

[0108] Blank group: no treatment with tisone + DHT, no sample treatment

[0109] Control group: treated with cortisone + DHT, no sample treatment

[0110] Experimental group: treated with cortisone + DHT, experimental group sample treatment

[0111] Test method: Take SZ95 cells and culture them in a T75 culture flask with DMEM medium. When the cell density is about 80%, inoculate them in a 24-well plate with a coverslip and culture them at 37°C and 5% CO2. After 24 hours, aspirate the supernatant, treat each well according to the above group settings, set 3 replicate wells for each group, and take 5 photos of each well randomly. Incubate in a 37°C, 5% CO2 incubator for 72 hours. Aspirate the supernatant, wash the cells twice, and add 4% paraformaldehyde to fix for 1 hour. After fixation, add 1μg / mL Nile red to each well and incubate in a 37°C, 5% CO2 incubator for 30 minutes, then observe and take photos under a fluorescence microscope, and finally use Image J software to semi-quantitatively analyze the fluorescence data of the photos.

[0112] According to the fluorescence data, the relative oil content of the blank group was set as 100%, and the data of other groups were normalized to obtain the relative oil content; the relative oil inhibition rate of the control group was set as 0%, and the data of other groups were normalized to obtain the relative oil inhibition rate. The t test was used for pairwise comparison, *P<0.05 indicated that the difference was statistically significant, **P<0.01 indicated that the difference was statistically significant, and ***P<0.001 indicated that the difference was extremely statistically significant.

[0113] The measurement results are shown in Table 3.

[0114] Table 3

[0115]

[0116] According to the analysis of Table 3, comparing the blank group and the control group, cortisone + DHT treatment can significantly enhance the oil content in SZ95 cells. Embodiments 8 and 9 show the effect of inhibiting the oil production induced by cortisone + DHT, and embodiments 1-7 all effectively inhibit the oil production induced by cortisone + DHT to varying degrees, and their inhibitory effects are significantly different from those of embodiments 8 and 9. Therefore, the brown rice fermentation filtrate and Ecdoin in the embodiment of the present application both have the effect of inhibiting the oil production induced by hormone stimulation, and the composition has a stronger effect of inhibiting oil production than a single substance, and has a synergistic effect.

[0117] Experimental Example 3: Effects of brown rice fermentation filtrate and ectoin on lipid production under fatty acid + hormone combined stimulation (LA and DHT)

[0118] Group settings:

[0119] Blank group: no LA and DHT co-treatment, no sample treatment

[0120] Control group: LA and DHT co-treatment, no sample treatment

[0121] Experimental group: LA and DHT were co-treated, and the experimental group samples were treated

[0122] Test method: Take SZ95 cells and culture them in a T75 culture flask with DMEM medium. When the cell density is about 80%, inoculate them in a 24-well plate with a coverslip and culture them at 37°C and 5% CO2. After 24 hours, aspirate the supernatant, treat each well according to the above group settings, set 3 replicate wells for each group, and take 5 photos of each well randomly. Incubate in a 37°C, 5% CO2 incubator for 72 hours. Aspirate the supernatant, wash the cells twice, and add 4% paraformaldehyde to fix for 1 hour. After fixation, add 1μg / mL Nile red to each well and incubate in a 37°C, 5% CO2 incubator for 30 minutes, then observe and take photos under a fluorescence microscope, and finally use Image J software to semi-quantitatively analyze the fluorescence data of the photos.

[0123] According to the fluorescence data, the relative oil content of the blank group was set as 100%, and the data of other groups were normalized to obtain the relative oil content; the relative oil inhibition rate of the control group was set as 0%, and the data of other groups were normalized to obtain the relative oil inhibition rate. The t test was used for pairwise comparison, *P<0.05 indicated that the difference was statistically significant, **P<0.01 indicated that the difference was statistically significant, and ***P<0.001 indicated that the difference was extremely statistically significant.

[0124] The measurement results are shown in Table 4.

[0125] Table 4

[0126]

[0127] According to the analysis in Table 4, comparing the blank group and the control group, LA+DHT treatment can significantly enhance the oil content in SZ95 cells. Examples 8 and 9 showed the efficacy of inhibiting LA+DHT-induced oil production. At the same time, Examples 1-7 all effectively inhibited LA+DHT-induced oil production to varying degrees, and their inhibitory effects were significantly different from those of Examples 8 and 9. Therefore, the brown rice fermentation filtrate and ectoine in the examples of the present application both inhibited oil production induced by fatty acid + hormone compound stimulation, and the combination had a stronger inhibitory effect on oil production than a single substance, and had a synergistic effect.

[0128] Experimental Example 4: Effects of brown rice fermentation filtrate and ectoin on lipid production under fatty acid + hormone combined stimulation (AA and cortisone)

[0129] Group settings:

[0130] Blank group: no AA and cortisone co-treatment, no sample treatment

[0131] Control group: AA and cortisone co-treatment, no sample treatment

[0132] Experimental group: AA and cortisone were co-treated, and the experimental group samples were treated

[0133] Test method: Take SZ95 cells and culture them in a T75 culture flask with DMEM medium. When the cell density is about 80%, inoculate them in a 24-well plate with a coverslip and culture them at 37°C and 5% CO2. After 24 hours, aspirate the supernatant, treat each well according to the above group settings, set 3 replicate wells for each group, and take 5 photos of each well randomly. Incubate in a 37°C, 5% CO2 incubator for 72 hours. Aspirate the supernatant, wash the cells twice, and add 4% paraformaldehyde to fix for 1 hour. After fixation, add 1μg / mL Nile red to each well and incubate in a 37°C, 5% CO2 incubator for 30 minutes, then observe and take photos under a fluorescence microscope, and finally use Image J software to semi-quantitatively analyze the fluorescence data of the photos.

[0134] According to the fluorescence data, the relative oil content of the blank group was set as 100%, and the data of other groups were normalized to obtain the relative oil content; the relative oil inhibition rate of the control group was set as 0%, and the data of other groups were normalized to obtain the relative oil inhibition rate. The t test was used for pairwise comparison, *P<0.05 indicated that the difference was statistically significant, **P<0.01 indicated that the difference was statistically significant, and ***P<0.001 indicated that the difference was extremely statistically significant.

[0135] The measurement results are shown in Table 5.

[0136] Table 5

[0137] Is it stimulating? name Oil content Oil inhibition rate p value (vs control group) p value (vs Example 8) Blank group no BL 100％ - 0.0003(***) - Control group yes CN 130％ 0％ - - Example 1 yes T1 114％ 16％ 0.0079(**) 0.0321(*) Example 2 yes T2 110％ 20％ 0.0072(**) 0.0133(*) Example 3 yes T3 111％ 19％ 0.0066(**) 0.0217(*) Example 4 yes T4 104％ 26％ 0.0038(**) 0.0058(**) Example 5 yes T5 107％ 23％ 0.0043(**) 0.0093(**) Example 6 yes T6 111％ 19％ 0.0057(**) 0.0355(*) Example 7 yes T7 115％ 15％ 0.0082(**) 0.0428(*) Example 8 yes T8 119％ 11％ 0.0181(*) - Example 9 yes T9 121％ 9％ 0.0256(*) -

[0138] According to Table 5 analysis, comparing the blank group and the control group, AA+ cortisone treatment can significantly enhance the oil content in SZ95 cells. Embodiments 8 and 9 show the effect of inhibiting the oil production induced by AA+ cortisone, and embodiments 1-7 all effectively inhibit the oil production induced by AA+ cortisone to varying degrees, and there are significant differences in its inhibitory effect with embodiments 8 and 9. Therefore, the brown rice fermentation filtrate and Ecdoin in the embodiment of the present application all have the effect of inhibiting the oil production induced by the compound stimulation of fatty acid+hormone, and the composition has a stronger effect of inhibiting oil production than a single substance, and has a synergistic effect.

[0139] Experimental Example 5: Effects of brown rice fermentation filtrate and ectoin on lipid production under urban dust standard material (SRM 1649B) and ultraviolet (UV) stimulation

[0140] Group settings:

[0141] Blank group: no 1649B and UV co-treatment, no sample treatment

[0142] Control group: 1649B and UV co-treatment, no sample treatment

[0143] Experimental group: 1649B and UV treatment, experimental group sample treatment

[0144] Test method: Take SZ95 cells and culture them in a T75 culture flask with DMEM medium. When the cell density is about 80%, inoculate them in a 24-well plate with a coverslip and culture them at 37°C and 5% CO2. After 24 hours, aspirate the supernatant, treat each well according to the above group settings, set 3 replicate wells for each group, and take 5 photos of each well randomly. Incubate in a 37°C, 5% CO2 incubator for 72 hours. Aspirate the supernatant, wash the cells twice, and add 4% paraformaldehyde to fix for 1 hour. After fixation, add 1μg / mL Nile red to each well and incubate in a 37°C, 5% CO2 incubator for 30 minutes, then observe and take photos under a fluorescence microscope, and finally use Image J software to semi-quantitatively analyze the fluorescence data of the photos.

[0145] According to the fluorescence data, the relative oil content of the blank group was set as 100%, and the data of other groups were normalized to obtain the relative oil content; the relative oil inhibition rate of the control group was set as 0%, and the data of other groups were normalized to obtain the relative oil inhibition rate. The t test was used for pairwise comparison, *P<0.05 indicated that the difference was statistically significant, **P<0.01 indicated that the difference was statistically significant, and ***P<0.001 indicated that the difference was extremely statistically significant.

[0146] The measurement results are shown in Table 6.

[0147] Table 6

[0148]

[0149]

[0150] According to the analysis in Table 6, comparing the blank group and the control group, 1649B+UV treatment can significantly enhance the lipid content in SZ95 cells. However, Examples 1, 2, 8, and 9 did not show significant inhibitory effects on lipid production induced by 1649B+UV. Therefore, brown rice fermentation filtrate, Ecdoin and its combination could not inhibit lipid production, indicating that unlike the hormone stimulation and fatty acid stimulation mentioned above, brown rice fermentation filtrate, Ecdoin and its combination had no inhibitory effects on lipid production under 1649B+UV stimulation.

Claims

1. Use of rice fermentation product filtrate and ectoine in inhibiting and / or reducing sebum production, wherein the sebum production is sebum production caused by fatty acids and hormones; Preferably, the fatty acid comprises arachidonic acid and / or linoleic acid; Preferably, the hormone includes one or more of cortisone, 5α-dihydrotestosterone, androgen, estrogen, glucocorticoid, and adrenal androgen.

2. The use according to claim 1, wherein the mass ratio of the rice fermentation product filtrate to the ectoine is not less than 1; Preferably, the mass ratio of the rice fermentation product filtrate to the ectoine is (1-200000):1, preferably (200-5000):1, and more preferably (200-1000):

1.

3. The use according to claim 1 or 2, wherein the rice fermentation product filtrate comprises yeast / rice fermentation product filtrate.

4. The use according to any one of claims 1 to 3, wherein the rice fermentation product filtrate and ectoine are used in preventing and / or alleviating excessive sebum secretion; Preferably, the rice fermentation product filtrate and ectoine are used to prevent and / or alleviate the formation of one or more of acne, pimples, acne, blackheads, inflammatory papules, pustules, enlarged pores, dull skin color, nodules, abscesses, and dandruff.

5. The use according to any one of claims 1-4, wherein the rice fermentation product filtrate and ectoine are used in the preparation of an oil control active agent.

6. The use according to any one of claims 1-4, wherein the rice fermentation product filtrate and ectoine are used as oil control active agents.

7. Use of rice fermentation product filtrate in inhibiting and / or reducing sebum production, wherein the sebum production is sebum production caused by fatty acids and hormones; Preferably, the rice fermentation product filtrate comprises yeast / rice fermentation product filtrate; Preferably, the fatty acid comprises arachidonic acid and / or linoleic acid; Preferably, the hormone includes one or more of cortisone, 5α-dihydrotestosterone, androgen, estrogen, glucocorticoid, and adrenal androgen.

8. The use according to claim 7, wherein the rice fermentation product filtrate is used for preventing and / or alleviating excessive sebum secretion; Preferably, the rice fermentation product filtrate is used to prevent and / or alleviate the formation of one or more of acne, pimples, acne, blackheads, inflammatory papules, pustules, enlarged pores, dull skin color, nodules, abscesses, and dandruff.

9. The use according to claim 7 or 8, wherein the filtrate of the rice fermentation product is used for preparing an oil-controlling active agent.

10. The use according to claim 7 or 8, wherein the filtrate of the rice fermentation product is used as an oil control active agent.

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