Pantoea sp. RSD6 and application thereof in plant disease control

Abstract

The invention discloses pantoea sp. RSD6 and application of the pantoea sp. RSD6 in prevention and treatment of plant diseases. The invention provides Pantoea sp., the strain number of the Pantoea sp. Is RSD6, and the preservation number of the Pantoea sp. In the China General Microbiological Culture Collection Center is CGMCC (China General Microbiological Culture Collection Center) 31074. When germinated rice seeds are damaged by pathogenic bacteria B. plantarri, the strain RSD6 can restore the germination rate, the root length and the bud length of the germinated rice seeds to normal levels. The pantoea sp. RSD6 can enhance the resistance of rice seedlings to B. plantarri in the growth and development process. When rice seedlings are damaged by pathogenic bacteria B. plantarri, the strain RSD6 can restore the growth condition of rice plants to a normal level. That is to say, the strain RSD6 can prevent or control diseases caused by the damage of the original bacterium B. plantarri to the rice.

Description

Technical Field

[0001] The present invention relates to the field of microorganisms, in particular to Pantoea RSD6 and application thereof in plant disease prevention and treatment. Background Art

[0002] At present, the application of antibiotics and metal ions is still the main means to solve plant bacterial diseases. With the widespread application of these two methods around the world, more and more antibiotic and metal ion tolerant pathogens have begun to appear on a large scale. Therefore, the exploration of new solutions is urgent.

[0003] Burkholderia phytopathogenes is one of the main pathogenic members of the genus Burkholderia. Rice bacterial blight caused by Burkholderia phytopathogenes is an increasingly serious disease problem in rice production worldwide. At present, most rice-growing areas in my country are affected and invaded by this pathogen, such as most southern provinces and the three northeastern provinces. At present, there are no reports on the use of Pantoea to control Burkholderia phytopathogenes.

[0004] Long-term use of chemical pesticides and antibiotics, on the one hand, will make pests or pathogens develop strong drug resistance, which is not conducive to subsequent management. On the other hand, it is also toxic to beneficial organisms and causes serious damage to the surrounding environment, which is not conducive to protecting the diversity of the ecological environment. Biological control has many advantages over chemical prevention methods, such as less pollution to the environment, low cost, safety for humans and animals, and pests and diseases are not easy to develop resistance. Energy-saving biological control has become the first choice for disease prevention and control today, and biocontrol bacteria are a very important part of biological control methods. . Summary of the invention

[0005] The invention aims to provide a Pantoea RSD6 and application thereof in plant disease prevention and treatment.

[0006] The invention provides a Pantoea sp., wherein the Pantoea sp. is Pantoea sp., the strain number of which is RSD6, and the preservation number of which in the General Microbiological Center of China National Committee for Microbiological Culture Collection is CGMCC No. 31074.

[0007] The present invention provides a bacterial agent, which contains the above-mentioned Pantoea and / or a culture containing the above-mentioned Pantoea.

[0008] The active ingredients of the above-mentioned bacterial agent may be the above-mentioned Pantoea and / or the culture of the above-mentioned Pantoea (such as fermentation products). The active ingredients of the above-mentioned bacterial agent may also contain other biological components or non-biological components. Those skilled in the art may determine the other active ingredients of the above-mentioned bacterial agent according to the effects of the bacterial agent.

[0009] The term "culture" refers to a liquid or solid product (all substances in the culture container, fermentation products) that has a microbial community after artificial inoculation and culture. That is, the product obtained by growing and / or amplifying microorganisms, which can be a biologically pure culture of microorganisms, or it can contain a certain amount of culture medium, metabolites or other components produced during the culture process.

[0010] The application of the above-mentioned pantoea or the above-mentioned bacterial agent in any of the following should also be within the scope of protection of the present invention:

[0011] A1) Use of the above-mentioned Pantoea in the preparation of plant pathogen inhibitors;

[0012] A2) Use of the above-mentioned Pantoea or the above-mentioned bacterial agent in inhibiting plant pathogens;

[0013] A3) Use of the above-mentioned Pantoea in the preparation of products for preventing and / or treating plant diseases;

[0014] A4) Use of the above-mentioned Pantoea or the above-mentioned bacterial agent in preventing and / or treating plant diseases.

[0015] Furthermore, the plant is a monocotyledonous plant.

[0016] Furthermore, the monocotyledonous plant is the following 1), 2) or 3):

[0017] 1) Gramineae;

[0018] 2) Oryza;

[0019] 3) Rice.

[0020] Furthermore, it is characterized in that: the pathogen in A1) or A1) is Burkholderia.

[0021] The present invention provides a method for preventing and / or treating plant diseases, which comprises culturing the above-mentioned Pantoea in a microbial culture medium, collecting the culture (fermentation product), and treating plants with the culture.

[0022] Furthermore, the plant disease is a disease caused by Burkholderia.

[0023] Furthermore, the plant is a monocotyledonous plant.

[0024] Furthermore, the monocotyledonous plant is the following 1), 2) or 3):

[0025] 1) Gramineae;

[0026] 2) Oryza;

[0027] 3) Rice.

[0028] The beneficial effects of the present invention are: Pantoea RSD6 can directly antagonize B.plantarri on a plate, and the fermentation liquid of RSD6 has a phenotype that inhibits the growth of B.plantarri. Pantoea RSD6 can enhance the resistance of rice seeds to B.plantarri during the pre-germination process. When the germinating rice seeds are attacked by the pathogen B.plantarri, the strain RSD6 can restore the germination rate, root length and shoot length to normal levels. Pantoea RSD6 can enhance the resistance of rice seedlings to B.plantarri during growth and development. When the rice seedlings are attacked by the pathogen B.plantarri, the strain RSD6 can restore the growth of the rice plants to normal levels. In other words, the strain RSD6 can prevent or control diseases caused by the pathogen B.plantarri attacking rice.

[0029] Collection Instructions

[0030] Biomaterials

[0031] Classification and nomenclature of biological materials: Pantoea sp.

[0032] Strain number of biological material: RSD6.

[0033] Name of the depository unit of biological materials: General Microbiology Center of China Microbiological Culture Collection Administration.

[0034] The abbreviation of the depository of biological materials is: CGMCC.

[0035] Address of the depository of biological materials: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, China Institute, Postal Code: 100101.

[0036] Deposit date of biological material: July 23, 2024.

[0037] The registration number of the Collection Center for Biological Materials is: CGMCC No.31074. BRIEF DESCRIPTION OF THE DRAWINGS

[0038] Figure 1 Figure a shows that Pantoea RSD6 has an antagonistic effect on B. plantarri directly on MA plates, and figure b shows the antibacterial effect of the fermentation broth of strain RSD6 on B. plantarri.

[0039] Figure 2 In the figure a, the phenotypic result is represented; in the figure b, the germination rate of rice seeds in each experimental group is statistically analyzed by One-way ANOVA. The results with significant differences are shown as follows: * represents the significant analysis result of P < 0.05; ** represents the significant analysis result of P < 0.01; *** represents the significant analysis result of P < 0.001; **** represents the significant analysis result of P < 0.0001.

[0040] Figure 3 In the figure, a represents the phenotypic result, b and c represent the statistical results of root length and sprout length of germinated rice seeds in each experimental group, respectively. The statistical results were analyzed by One-way ANOVA, and the results with significant differences are shown as follows: * represents the significant analysis result of P < 0.05; ** represents the significant analysis result of P < 0.01; *** represents the significant analysis result of P < 0.001; **** represents the significant analysis result of P < 0.0001.

[0041] Figure 4 In the figure, a represents the phenotypic result, and b represents the statistical results of the height of rice seedlings in each experimental group. The statistical results were analyzed by One-way ANOVA, and the results with significant differences are shown: * represents the significant analysis result of P < 0.05; ** represents the significant analysis result of P < 0.01; *** represents the significant analysis result of P < 0.001; **** represents the significant analysis result of P < 0.0001. c represents the disease status of the plant, where the higher the disease index, the more serious the disease status. DETAILED DESCRIPTION

[0042] The present invention is further described in detail below in conjunction with specific embodiments, and the examples provided are only for illustrating the present invention, rather than for limiting the scope of the present invention. The examples provided below can be used as a guide for further improvements by those of ordinary skill in the art, and do not constitute a limitation of the present invention in any way.

[0043] The experimental methods in the following examples, unless otherwise specified, are all conventional methods, and are performed according to the techniques or conditions described in the literature in the field or according to the product instructions. The materials, reagents, etc. used in the following examples, unless otherwise specified, can all be obtained from commercial channels.

[0044] Unless otherwise specified, the quantitative tests in the following examples were performed three times and the results were averaged.

[0045] The B. plantarri ZJ171 in the following example is disclosed in the document “Matsumoto, H., Fan, X., Wang, Y. et al. Bacterial seed endophyte shapes disease resistance in rice. Nat. Plants 7, 60–72 (2021)”, and the public can obtain it from the inventor to repeat the experiment.

[0046] Example 1 Preparation of Pantoea sp.RSD6

[0047] Pantoea RSD6 was obtained from wild-type Nipponbare rice seeds by plate isolation culture.

[0048] Through microscopic and plate observation, it was found that the colony of RSD6 was light yellow and round with a smooth surface; the bacterial single cell morphology was rod-shaped, and there were irregular lines on the surface of the bacteria. The sequenced bacterial 16S rRNA gene sequence was compared by Blast in NCBI to obtain the comparison strains of bacteria, and the patent procedure was preserved. The specific information is as follows: Pan-bacteria RSD6 is registered with the General Microbiology Center of the China Microbiological Culture Collection Administration as CGMCC No.31074. The strain was deposited in the General Microbiology Center of the China Microbiological Culture Collection Administration (CGMCC) on July 23, 2024, and the preservation address is No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing.

[0049] The physiological and biochemical properties of strain RSD6 were determined, and the identification results showed that strain RSD6 was positive in the contact enzyme test, indicating that the strain can produce catalase; the results of the sugar fermentation experiment showed that strain RSD6 can ferment a variety of sugars including glucose, lactose, mannitol, fructose, trehalose, sucrose and maltose; it was negative in the gelatin experiment and soluble starch experiment, indicating that it does not produce amylase and gelatinase; the result was negative in the hydrogen sulfide gas generation experiment, indicating that strain RSD6 does not produce hydrogen sulfide; in the amino acid hydrolysis experiment, the experimental results of strain RSD6 for arginine, lysine and ornithine were all negative, indicating that strain RSD6 cannot utilize these amino acids; it showed positive reactions in the indole experiment and VP experiment, and negative reactions in the methyl red experiment and citrate experiment.

[0050] Test items Experimental Project Results Glucose glucose + Maltose maltose + Lactose lactose + Mannitol Mannitol + Surcrose sucrose + Mycose Trehalose + Fructose D-Fructose + Starch soluble Soluble starch － Catalase test Catalase (hydrogen peroxide) + Hydrogen Sulfide Test Hydrogen sulfide － Arginine test Arginine － Lysine test Lysine － Ornithine test Ornithine － Gelatin liquefaction test Gelatin experiment － Indole test Indole test + Methylred reaction test Methyl red test － VP test VP test + Citrate Citrate －

[0051] Among them, "+" indicates positive, and "-" indicates negative.

[0052] 1. Preparation of culture medium used for isolation and culture of strain RSD6:

[0053] MA selection medium containing Tropolone: ​​1g diammonium phosphate, 0.2g potassium chloride, 0.2g magnesium sulfate heptahydrate, 10g glucose, 10g agar powder, 20mg Tropolone, 100mg nystatin, adjust pH to 7.2±0.2, 10g agar powder, add ddH2O to make up to 1L.

[0054] LB selection medium containing Tropolone: ​​5 g yeast extract, 10 g tryptone, 10 g sodium chloride, 20 mg Tropolone, 100 mg nystatin, adjust pH to 7.3 ± 0.2, 10 g agar powder, add ddH2O to make up to 1 L.

[0055] 2. Experimental Methods

[0056] 2.1 Isolation and screening of antagonistic strains

[0057] Take 20 Nipponbare rice seeds, add 20 mL of sterile ddH2O to the material and shake and wash for 1 min. After cleaning the impurities and dust on the surface, remove the supernatant, add 25 mL of PBS and shake and soak the rice seeds for a period of time. Then, break the seeds in a sterile clean bench and dilute the sample to 10 with PBS by 10-fold dilution method. 2 , 10 3 and 10 4 The dilutions of different concentrations were spread on the selection medium of MA and LB containing Tropolone, and then cultured in an incubator at 28°C.

[0058] 2.2 Antagonistic experiment between strain RSD6 and pathogens

[0059] Take the bacterial solution of strain RSD6 in the logarithmic growth phase, centrifuge at 12,000rpm for 2 minutes, remove most of the supernatant and mix well for later use. First, evenly spread the pathogen indicator strain on the MA solid medium at an appropriate concentration. After the bacterial solution on the plate is completely dry, take 1μL of the mixed RSD6 bacterial solution and dot it in the center of the plate. Finally, place it in a 28℃ incubator and observe the results after two days.

[0060] 2.3 Gelatin experiment

[0061] Take gelatin culture medium and inoculate the target strain by puncture inoculation. After inoculation, place it in a 28℃ incubator for observation. Observe whether the gelatin culture medium is liquefied. If the culture medium is liquefied, it means that the bacteria can hydrolyze gelatin, which is positive.

[0062] 2.4 Amino acid hydrolysis experiment

[0063] Take the amino acid culture medium and inoculate the seed liquid of the target strain. After inoculation, place it in a 28°C incubator for observation. Observe whether the color of the liquid culture medium changes to purple. If there is a color change, it means that the bacteria can hydrolyze the amino acid, which is positive.

[0064] 2.5 Starch hydrolysis experiment

[0065] Streak the target strain on starch agar medium, place it in a 28℃ incubator and grow it for 48 hours, then add Lugol's solution to the plate and observe the results of the starch hydrolysis experiment. If it does not turn blue when exposed to iodine solution, the target strain can produce amylase to hydrolyze starch, which is positive.

[0066] 2.6 Sugar fermentation experiment

[0067] Add 400 μL of 10% various sugar solutions (glucose, mannitol, sucrose, trehalose, fructose, maltose, lactose) to the LB liquid medium containing bromocresol purple. Transfer the seed liquid of the strain to the sugar-containing medium and culture for 48 hours. Observe the results. If the medium turns yellow, the strain is positive for fermenting the sugar.

[0068] 2.7 Indole experiment

[0069] Take LB liquid culture medium, inoculate the seed solution of the target strain, culture at 28℃ for 48h, take about 1mL of culture solution, slowly add 2 drops of indole reagent along the wall of the tube, shake gently, and observe the results after standing for a while. If the solution turns red, it is positive.

[0070] 2.8 MR reaction determination

[0071] Take LB liquid medium with 1% glucose, inoculate the seed liquid of the target strain, culture at 28℃ for 48h, take about 1mL of the culture liquid, add 3 drops of methyl red reagent, and observe the results. If the liquid culture medium turns red, it is positive.

[0072] 2.9 VP reaction assay

[0073] Take LB liquid culture medium containing 1% glucose, inoculate the seed liquid of the target strain, and culture it at 28°C for 48 hours. Then take about 1 mL of culture liquid, add 3 drops of 6% α-naphthol alcohol solution, add 2 drops of 40% KOH, shake gently, and observe whether red appears after standing for a while. If red appears, it is positive.

[0074] 2.10 Catalase assay

[0075] Scrape some bacteria from the plate culture medium, apply it on a clean slide, then add 3% hydrogen peroxide to observe whether bubbles are generated. If bubbles are generated, the strain can produce catalase and is positive.

[0076] 2.11 Hydrogen sulfide experiment

[0077] Take the semi-solid medium of ferric ammonium citrate and inoculate the target strain by puncture inoculation. After inoculation, place it in a 28°C incubator for observation. Observe whether black is produced in the medium. If so, it means that the bacteria can produce hydrogen sulfide, which is positive.

[0078] 2.12 Observation of bacterial morphology using scanning electron microscopy

[0079] Sample preparation of strains:

[0080] (1) Immerse the sample in 2.5% glutaraldehyde solution and fix it at 4°C in a dark environment for more than 8 hours.

[0081] (2) After removing the glutaraldehyde fixative, soak the samples in deionized water for 6 min, 7 min, and 8 min respectively.

[0082] (3) Remove deionized water and soak the samples in 50%, 70%, 85%, and 95% ethanol for 14 min.

[0083] (4) Then change to 100% ethanol and soak for 15 minutes, and repeat this process three times.

[0084] Critical Point Drying:

[0085] (1) Open the air valve and press the Cooling button to cool to 10°C.

[0086] (2) Open the lid, spread the sample flat on the drying box, and tighten the lid fully.

[0087] (3) Press the medium in button to fill the sample tank with liquid CO2, press medium in again to stop, and wait for 27 minutes.

[0088] (4) Press medium out to drain all the liquid in the sample tank, and then press medium out to stop. Repeat steps (3) and (4) twice.

[0089] (5) Press medium in again. When the CO2 liquid is full, press medium in to stop. Press the Cooling button to stop cooling, and then press the heating button to start heating.

[0090] (6) After waiting for 45 minutes, close the main gas valve, press gas out, and adjust the steel ball to the 20 mark.

[0091] (7) After the air pressure drops to 50, press the heating button to turn it off. Wait for all the liquid to flow out. When the steel ball cannot be adjusted, take out the sample.

[0092] Next, spread the sample flat on a metal table. After gold spraying is completed, the sample can be placed under a scanning electron microscope for observation.

[0093] Example 2 Application of Pantoea RSD6 in the Control of Rice Burkholderia

[0094] 1. Preparation of MA solid and liquid culture medium for the culture of strains B. plantarri and RSD6:

[0095] Preparation method of MA solid culture medium: dissolve 1 g of ammonium dihydrogen phosphate (NH4H2PO4), 0.2 g of potassium chloride (KCl), 0.2 g of magnesium sulfate heptahydrate (MgSO4-7H2O), 10 g of glucose (Glucose), and 10 g of agar (Agar) in distilled water, make up to 1 L, and adjust the pH to 7.2±0.2.

[0096] Preparation method of MA liquid culture medium: dissolve 1g of diammonium phosphate (NH4H2PO4), 0.2g of potassium chloride (KCl), 0.2g of magnesium sulfate heptahydrate (MgSO4-7H2O), and 10g of glucose (Glucose) in distilled water, make up to 1L, and adjust the pH to 7.2±0.2.

[0097] 2. Strain culture, bacterial collection, antagonism and inhibition zone experiments

[0098] (1) Pick a single colony from each of the MA plates of B. plantarri ZJ171 and Pantoea sp. RSD6, transfer them to a test tube with 3 mL of MA liquid medium, and culture at 28°C and 220 rpm until the OD 600 = about 1;

[0099] (2) Centrifuge 1 mL of bacterial solution at 8,000 rpm for 5 min and collect the supernatant and precipitated bacteria;

[0100] (3) Add 1 mL of PBS to the bacteria and mix well with a pipette. The concentration of the bacterial solution is recorded as 10 9 CFU / mL;

[0101] (4) Then, the bacterial solution was diluted 10 times. 6 CFU / mL;

[0102] (5) Take 100 μL of a concentration of 10 6 CFU / mL of B. plantarri bacterial solution were used for plate antagonism experiment and inhibition zone experiment.

[0103] The results are as follows Figure 1 As shown, Figure 1 Figure a shows that Pantoea RSD6 has an antagonistic effect on B. plantarri directly on MA plates, and figure b shows the antibacterial effect of the fermentation broth of strain RSD6 on B. plantarri.

[0104] 3. Method for inoculating rice seeds with B. plantarri ZJ171 and Pantoea sp. RSD6:

[0105] (1) Select 50 Nipponbare rice seeds with full grains and dispense them into 50 mL centrifuge tubes for later use;

[0106] (2) In a sterile clean bench, add 25 mL of 70% ethanol to the rice seeds and mix for 1 minute;

[0107] (3) Remove the supernatant, add 25 mL of 30% sodium hypochlorite, and wash on a vertical stirrer at 30 rpm for 30 minutes;

[0108] (4) Remove the supernatant, add 25 mL of ddH2O, and wash on a vertical stirrer at 30 rpm for 10 min;

[0109] (5) discard the supernatant and repeat step (4) at least three times to remove the residual sodium hypochlorite;

[0110] (6) Finally, 10 mL of ddH2O was added to the surface-sterilized rice seeds, and the strains B. plantarri and RSD6 were added to the following groups at a final concentration of 10^6 CFU / mL: CK group (no bacteria), BP group (only B. plantarri was added), BP+RSD6 (strains B. plantarri and Pantoea RSD6 were added), and RSD6 group (only Pantoea RSD6 was added) and mixed;

[0111] (7) transferring the bacterial aqueous solution and rice seeds into a disposable culture dish and placing them in a dark environment for pre-germination;

[0112] (8) Finally, the germinated rice seeds that were pre-germinated for 3 days were transplanted into soil for cultivation, and their growth phenotypes were observed;

[0113] The results are as follows Figure 2-4 As shown, Figure 2 In the figure a, the phenotypic result is represented; in the figure b, the germination rate of rice seeds in each experimental group is statistically analyzed by One-way ANOVA. The results with significant differences are shown as follows: * represents the significant analysis result of P < 0.05; ** represents the significant analysis result of P < 0.01; *** represents the significant analysis result of P < 0.001; **** represents the significant analysis result of P < 0.0001.

[0114] Figure 3 In the figure, a represents the phenotypic result, b and c represent the statistical results of root length and sprout length of germinated rice seeds in each experimental group, respectively. The statistical results were analyzed by One-way ANOVA, and the results with significant differences are shown as follows: * represents the significant analysis result of P < 0.05; ** represents the significant analysis result of P < 0.01; *** represents the significant analysis result of P < 0.001; **** represents the significant analysis result of P < 0.0001.

[0115] Figure 4 In the figure, a represents the phenotypic result, and b represents the statistical results of the height of rice seedlings in each experimental group. The statistical results were analyzed by One-way ANOVA, and the results with significant differences are shown: * represents the significant analysis result of P < 0.05; ** represents the significant analysis result of P < 0.01; *** represents the significant analysis result of P < 0.001; **** represents the significant analysis result of P < 0.0001. c represents the disease status of the plant, where the higher the disease index, the more serious the disease status.

[0116] Combined with the above results, it can be seen that Pantoea RSD6 can directly antagonize B.plantarri on the plate, and the fermentation liquid of RSD6 has a phenotype that inhibits the growth of B.plantarri. Pantoea RSD6 can enhance the resistance of rice seeds to B.plantarri during the pre-germination process. When the germinating rice seeds are attacked by the pathogen B.plantarri, the strain RSD6 can restore the germination rate, root length and shoot length to normal levels. Pantoea RSD6 can enhance the resistance of rice seedlings to B.plantarri during growth and development. When the rice seedlings are attacked by the pathogen B.plantarri, the strain RSD6 can restore the growth of the rice plants to normal levels. In other words, the strain RSD6 can prevent or control the diseases caused by the pathogen B.plantarri invading rice.

[0117] The present invention has been described in detail above. It will be apparent to those skilled in the art that the present invention may be implemented in a wide range under equivalent parameters, concentrations and conditions without departing from the spirit and scope of the present invention and without the need for unnecessary experimentation. Although the present invention provides specific embodiments, it should be understood that further improvements may be made to the present invention. In short, according to the principles of the present invention, this application intends to include any changes, uses or improvements to the present invention, including changes made by conventional techniques known in the art that depart from the scope disclosed in this application. Applications of some of the basic features may be made within the scope of the following appended claims.

Claims

1. Pantoea, the Pantoea is Pantoea sp., the strain number of which is RSD6, and the deposit number of which in the General Microbiological Center of China National Microbiological Culture Collection Committee is CGMCC No.31074.

2. A microbial agent, characterized in that: The bacterial agent contains the Pantoea described in claim 1 and / or contains a culture of the Pantoea described in claim 1.

3. Use of the pantoea according to claim 1 or the bacterial agent according to claim 2 in any of the following: A1) Use of the Pantoea according to claim 1 in the preparation of plant pathogen inhibitors; A2) Use of the Pantoea described in claim 1 or the bacterial agent described in claim 2 in inhibiting plant pathogens; A3) Use of the Pantoea according to claim 1 in the preparation of products for preventing and / or treating plant diseases; A4) Use of the Pantoea according to claim 1 or the bacterial agent according to claim 2 in preventing and / or treating plant diseases.

4. The use according to claim 3, characterized in that: The plant is a monocotyledonous plant.

5. The use according to claim 4, characterized in that: The monocotyledonous plant is the following 1), 2) or 3): 1) Gramineae; 2) Oryza; 3) Rice.

6. The use according to any one of claims 3 to 5, characterized in that: A1) or the pathogenic bacteria described in A1) is Burkholderia.

7. A method for preventing and / or treating plant diseases, characterized in that: The method comprises culturing the Pantoea of ​​claim 1 in a microbial culture medium, collecting the culture (fermentation product), and treating plants with the culture.

8. The method according to claim 7, characterized in that: The plant disease is a disease caused by Burkholderia.

9. The method according to claim 7, characterized in that: The plant is a monocotyledonous plant.

10. The use according to claim 9, characterized in that: The monocotyledonous plant is the following 1), 2) or 3): 1) Gramineae; 2) Oryza; 3) Rice.

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