Protein worm nutritional solid granule production method

Abstract

The invention discloses a production method of protein worm nutritional solid granules, and relates to the technical field of health-care foods. The production method of the protein worm nutritional solid electuary comprises six steps, protein worms are adopted as protein raw materials, the content of protein in the protein worms is extremely high and far higher than that of traditional livestock and poultry products, and the protein worms are rich in various amino acids and trace elements necessary for the human body and are high-quality protein sources; according to the method, the selenium-rich soybean seeds are used as raw materials, crude vitamins, macromolecular proteins, saccharides and other substances in the selenium-rich soybean seeds are degraded, the product absorption efficiency of a user is improved, and probiotics are added, so that the intestinal microecological balance can be maintained, and the utilization rate of nutritional ingredients in the raw materials can be increased.

Description

Technical Field

[0001] The invention relates to the technical field of health food, in particular to a method for producing a protein worm nutritional solid granule. Background Art

[0002] Protein is an essential nutrient for the human body, and we need to pay attention to the intake of high-protein foods in our daily lives. Some special groups need high-protein intake, such as the weak and sick, pregnant women, and some athletes, but nowadays, high-protein intake requires cumbersome food preparation, which is not very convenient.

[0003] Protein worms are annelids of the genus Gastropoda of the order Oligochaeta, also known as dotted Gastropoda, red-spotted Gastropoda, and protein worms. The protein content in protein worms is extremely high, far exceeding that of traditional livestock and poultry products, and is rich in a variety of essential amino acids and trace elements for the human body. It is a high-quality source of protein. In the process of protein worm breeding, less waste is discharged, less pollution to the environment, and it can effectively transform agricultural waste (such as straw, fruit and vegetable residues) into high-protein feed, realizing the recycling of resources.

[0004] The invention with publication number CN104146272A discloses a high-protein supplement granule and a preparation method thereof. The raw materials of the high-protein supplement granule are main ingredients and auxiliary ingredients. The main ingredients include: soybeans, sesame seeds, pumpkin seeds, and spirulina; the auxiliary ingredients are sweet auxiliary ingredients or salty auxiliary ingredients. The sweet auxiliary ingredients include mango, banana, jackfruit, and sugar, and the salty auxiliary ingredients include carrots, cowpeas, onions, and salt.

[0005] As shown in the above invention, the existing high-protein supplement powder mainly uses soybeans, sesame seeds, pumpkin seeds and spirulina to supplement protein. The protein content in these raw materials is not high and cannot meet the supplement demand of high-quality protein. Summary of the invention

[0006] In view of the deficiencies in the prior art, the present invention provides a method for producing a protein worm nutritional solid granule, which solves the existing problems.

[0007] To achieve the above object, the present invention is implemented by the following technical scheme: a method for producing a protein worm nutrition solid granule, comprising the following steps:

[0008] Step 1: Prepare a certain amount of raw materials, including: selenium-enriched soybeans, dried protein worms, milk powder, probiotics, casein hydrolyzate, glucose and sodium chloride;

[0009] Step 2: Separately wash the selenium-rich soybeans and dried protein worms in an automatic washing machine, quickly spin dry, and send them to a low-temperature air-drying conveyor line to remove surface moisture, and then perform high-temperature dehydration and sterilization;

[0010] Step 3: Send the processed selenium-enriched soybeans and dried protein worms into a grinder and grind them into powder;

[0011] Step 4: adding soybean powder, sodium chloride, glucose and water by weight into a fermentation tank, sterilizing and then adding probiotics for fermentation and cultivation, concentrating the fermentation liquid by inorganic ceramic membrane microfiltration, and then drying by low-temperature fluidized bed to obtain concentrated crystals;

[0012] Step 5, feeding the above-mentioned concentrated crystals, protein insect powder, milk powder and casein hydrolyzate into a pulverizer according to weight components, stirring and mixing to make powder, and sterilizing in an oven to obtain a powdered solid finished product;

[0013] Step 6: Finally, the finished products are automatically vacuum-packed by an automatic packaging machine and stored.

[0014] Preferably, the weight percentages of the raw materials in step one are: 15-25% selenium-enriched soybeans, 40-50% dried protein worms, 15-25% milk powder, 1-2% probiotics, 7-9% casein hydrolyzate, 1-3% glucose and 3-5% sodium chloride.

[0015] Preferably, in step 2, the selenium-rich soybeans and dried protein worms are washed and then rapidly dehydrated, dried and sterilized at a high temperature of 100-400° C. in a large microwave oven.

[0016] Preferably, the sterilization temperature in step 4 is 121-126° C. and the time is 30-35 min.

[0017] Preferably, in step 4, the fermentation culture temperature is 34-37°C, the initial pH is 7-8, the stirring speed is 120-140r / min, the ratio of the volume of the fermentation liquid to the volume of the air introduced per minute is 1:0.4-0.6, and the time is 28-30h.

[0018] Preferably, in step 4, the pore size of the inorganic ceramic membrane tube is 0.2 μm, the concentration pressure is 0.15-0.18 MPa, and the membrane area is 0.25 m.

[0019] Preferably, in step 4, the drying air inlet temperature of the low-temperature fluidized bed is 40-60° C., the wind speed is 1.8-2.2 m / s, and the drying time is 40-70 min.

[0020] Preferably, the temperature of the oven in step 5 is 120-200° C. and the time is 30-90 minutes.

[0021] Beneficial Effects

[0022] The present invention provides a method for producing a protein worm nutritional solid granule. Compared with the prior art, it has the following beneficial effects:

[0023] 1. The method for producing protein worm nutritional solid powder adopts protein worms as protein raw materials. The protein content in the protein worms is extremely high, far exceeding that of traditional livestock and poultry products. The protein worms are rich in multiple amino acids and trace elements necessary for the human body, and are a high-quality protein source. At the same time, the method adopts a fermentation method to degrade substances such as crude vitamins, macromolecular proteins, and sugars in selenium-rich soybeans, thereby improving the user's absorption efficiency of the product. Moreover, the method can not only maintain the balance of intestinal microecology, but also increase the utilization rate of nutrients in the raw materials by adding probiotics.

[0024] 2. The production method of the protein worm nutritional solid powder selects selenium-rich soybeans, dried protein worms, milk powder, probiotics, casein hydrolyzate, glucose and sodium chloride as raw materials, reasonably proportions the amount of each raw material, and uses standardized production and coordinated beneficial ingredients of each raw material to prepare a nutritional powder that can not only meet the human body's needs for multiple elements such as amino acids, calcium, and selenium, but also can adjust the human body's acidic constitution by eating this product, making the body healthier. BRIEF DESCRIPTION OF THE DRAWINGS

[0025] Figure 1 It is a schematic diagram of the structure of the method flow of the present invention;

[0026] Figure 2 This is a schematic diagram of how different concentrations of protein insect powder (Lf) of the present invention prevent the precipitation of CPPs. DETAILED DESCRIPTION

[0027] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0028] See Figure 1-2 , the present invention provides the following four technical solutions:

[0029] The first embodiment: a method for producing a protein worm nutrition solid granule, comprising the following steps:

[0030] Step 1: prepare a certain amount of raw materials, including: selenium-enriched soybeans, dried protein worms, milk powder, probiotics, casein hydrolysate, glucose, and sodium chloride, which include the following weight percentages: 15% of selenium-enriched soybeans, 40% of dried protein worms, 25% of milk powder, 2% of probiotics, 9% of casein hydrolysate, 3% of glucose, and 5% of sodium chloride;

[0031] Step 2: Selenium-rich soybeans and dried protein worms are cleaned separately in an automatic cleaning machine, quickly dried, and sent to a low-temperature air-drying conveyor line to remove surface moisture, and then quickly dehydrated, dried and sterilized at a high temperature of 200°C in a large microwave oven;

[0032] Step 3: Send the processed selenium-enriched soybeans and dried protein worms into a grinder and grind them into powder;

[0033] Step 4: Add soybean powder, sodium chloride, glucose and water by weight into a fermentation tank for sterilization at a temperature of 121°C for 30 minutes. After sterilization, add probiotics for fermentation culture at a temperature of 34°C, an initial pH of 7, a stirring speed of 120 r / min, a ratio of the volume of the fermentation liquid to the volume of the air introduced per minute of 1:0.4, and a time of 28 hours. Concentrate the fermentation liquid by microfiltration using an inorganic ceramic membrane tube, the pore size of the inorganic ceramic membrane tube is 0.2 μm, the concentration pressure is 0.15 MPa, and the membrane area is 0.25 m2. Then, the fermentation liquid is dried in a low-temperature fluidized bed, the drying air inlet temperature of the low-temperature fluidized bed is 40-60°C, the wind speed is 1.8-2.2 m / s, and the time is 40 minutes to obtain concentrated crystals.

[0034] Step 5, the concentrated crystals, protein insect powder, milk powder and casein hydrolyzate are sent into a pulverizer according to weight components, stirred and mixed to make powder, and sterilized in an oven at a temperature of 120° C. for 30 minutes to obtain a powdered solid finished product;

[0035] Step 6: Finally, the finished products are automatically vacuum-packed by an automatic packaging machine and stored.

[0036] In the above scheme, yellow soybeans from Xintian in May are selected and finely screened with a specific gravity screening machine to remove impurities and mildew; raw materials that do not meet the requirements in color and size are removed by a color sorter, and the raw material particles are carefully selected to achieve basically uniform size. The selected yellow soybeans are dried and peeled, which can reduce the heat-resistant bacteria brought in by the soil and improve the taste of the product.

[0037] In the above scheme, the casein hydrolysate contains casein phosphopeptides (CPPs) with a mass fraction of 15%.

[0038] Second embodiment: Another embodiment of the present invention discloses a method for producing a protein worm nutrition solid granule, comprising the following steps:

[0039] Step 1: prepare a certain amount of raw materials, including: selenium-enriched soybeans, dried protein worms, milk powder, probiotics, casein hydrolysate, glucose, and sodium chloride, which include the following weight percentages: 20% selenium-enriched soybeans, 45% dried protein worms, 20% milk powder, 1% probiotics, 8% casein hydrolysate, 2% glucose, and 4% sodium chloride;

[0040] Step 2: Selenium-rich soybeans and dried protein worms are cleaned separately in an automatic cleaning machine, quickly dried, and sent to a low-temperature air-drying conveyor line to remove surface moisture, and then quickly dehydrated, dried and sterilized at a high temperature of 300°C in a large microwave oven;

[0041] Step 3: Send the processed selenium-enriched soybeans and dried protein worms into a grinder and grind them into powder;

[0042] Step 4: Add soybean powder, sodium chloride, glucose and water by weight into a fermentation tank for sterilization at a temperature of 123°C for 33 minutes. After sterilization, add probiotics for fermentation culture at a temperature of 35°C, an initial pH of 7, a stirring speed of 130 r / min, a ratio of the volume of the fermentation liquid to the volume of the air introduced per minute of 1:0.5, and a time of 29 hours. Concentrate the fermentation liquid by microfiltration using an inorganic ceramic membrane tube, the pore size of the inorganic ceramic membrane tube is 0.2 μm, the concentration pressure is 0.16 MPa, and the membrane area is 0.25 m. Then, the fermentation liquid is dried in a low-temperature fluidized bed, the drying air inlet temperature of the low-temperature fluidized bed is 50°C, the wind speed is 2.0 m / s, and the time is 55 minutes to obtain concentrated crystals.

[0043] Step 5, the concentrated crystals, protein insect powder, milk powder and casein hydrolyzate are sent into a pulverizer according to weight components, stirred and mixed to make powder, and sterilized in an oven at a temperature of 160° C. for 60 minutes to obtain a powdered solid finished product;

[0044] Step 6: Finally, the finished products are automatically vacuum-packed by an automatic packaging machine and stored.

[0045] In the above scheme, yellow soybeans from Xintian in May are selected and finely screened with a specific gravity screening machine to remove impurities and mildew; raw materials that do not meet the requirements in color and size are removed by a color sorter, and the raw material particles are carefully selected to achieve basically uniform size. The selected yellow soybeans are dried and peeled, which can reduce the heat-resistant bacteria brought in by the soil and improve the taste of the product.

[0046] In the above scheme, the casein hydrolysate contains casein phosphopeptides (CPPs) with a mass fraction of 18%.

[0047] The third embodiment: Another embodiment of the present invention discloses a method for producing a protein worm nutrition solid granule, comprising the following steps:

[0048] Step 1: prepare a certain amount of raw materials, including: selenium-enriched soybeans, dried protein worms, milk powder, probiotics, casein hydrolysate, glucose, and sodium chloride, which include the following weight percentages: 25% of selenium-enriched soybeans, 45% of dried protein worms, 15% of milk powder, 2% of probiotics, 7% of casein hydrolysate, 3% of glucose, and 3% of sodium chloride;

[0049] Step 2: Selenium-rich soybeans and dried protein worms are cleaned separately in an automatic cleaning machine, quickly dried, and sent to a low-temperature air-drying conveyor line to remove surface moisture, and then quickly dehydrated, dried and sterilized at a high temperature of 400°C in a large microwave oven;

[0050] Step 3: Send the processed selenium-enriched soybeans and dried protein worms into a grinder and grind them into powder;

[0051] Step 4: Add soybean powder, sodium chloride, glucose and water by weight into a fermentation tank for sterilization at a temperature of 126°C for 35 minutes. After sterilization, add probiotics for fermentation culture at a temperature of 37°C, an initial pH of 8, a stirring speed of 140r / min, a ratio of the volume of the fermentation liquid to the volume of the air introduced per minute of 1:0.6, and a time of 30 hours. Concentrate the fermentation liquid by microfiltration using an inorganic ceramic membrane tube, the pore size of the inorganic ceramic membrane tube is 0.2μm, the concentration pressure is 0.18MPa, and the membrane area is 0.25m. Then, the fermentation liquid is dried in a low-temperature fluidized bed, the drying air inlet temperature of the low-temperature fluidized bed is 60°C, the wind speed is 2.2m / s, and the time is 70min to obtain concentrated crystals.

[0052] Step 5, the above-mentioned concentrated crystals, protein insect powder, milk powder and casein hydrolyzate are sent into a pulverizer according to weight components, stirred and mixed for powdering, and sterilized in an oven at a temperature of 200° C. for 90 minutes to obtain a powdered solid finished product;

[0053] Step 6: Finally, the finished products are automatically vacuum-packed by an automatic packaging machine and stored.

[0054] In the above scheme, yellow soybeans from Xintian in May are selected and finely screened with a specific gravity screening machine to remove impurities and mildew; raw materials that do not meet the requirements in color and size are removed by a color sorter, and the raw material particles are carefully selected to achieve basically uniform size. The selected yellow soybeans are dried and peeled, which can reduce the heat-resistant bacteria brought in by the soil and improve the taste of the product.

[0055] In the above scheme, the casein hydrolysate contains casein phosphopeptides (CPPs) with a mass fraction of 20%.

[0056] The above three implementation methods can all prepare protein worm nutritional solid granules.

[0057] Fourth implementation method: The effect of protein worm powder on the effect of CPPs in delaying calcium phosphate precipitation:

[0058] 1) Prepare 0.008 mol / L phosphate buffer:

[0059] Solution A: 0.08 mol / LNaH2PO4·2H2O solution, weigh 11.04 g of NaH2PO4·2H2O and dissolve it in 1000 ml of distilled water.

[0060] Solution B: 0.08 mol / LNa2HPO4·7H2O solution. Weigh 14.24 g of Na2HPO4·7H2O and dissolve it in 1000 ml of distilled water.

[0061] According to the required pH 8.0 (pH value in the small intestine), 5.3 ml of solution A and 94.7 ml of solution B were mixed and then diluted to 0.008 mol / L phosphate buffer according to the experimental requirements.

[0062] 2) Use 0.008 mol / L phosphate buffer to prepare 50 ml of lactoferrin solution with concentrations of 1.4, 1.6, 1.8, 2.0, and 2.2 mg / ml, respectively, divide it into two portions, put them in 25 ml volumetric flasks respectively, and use one portion as blank solution.

[0063] To the other portion, 25 mg of casein hydrolyzate (containing 5 mg of CPPs) was added to prepare 25 ml of a CPPs solution with a concentration of 0.2 mg / ml as the sample solution.

[0064] The prepared solutions were placed in a constant temperature water bath at 37°C for 5 h (accelerated experiment). The consumption of 0.8 mol / LCaCl2 solution was used as the ordinate and the concentration of Lf solution was used as the abscissa to plot the effect of different concentrations of lactoferrin on the ability of CPPs to delay calcium phosphate precipitation.

[0065] As can be seen from the figure, when the concentration is between 1.4 and 2.0, as the concentration of lactoferrin increases, it is beneficial for the combination of CPPs and calcium, thereby increasing the solubility of calcium salts in the alkaline environment of the small intestine and facilitating absorption.

[0066] Meanwhile, the contents not described in detail in this specification belong to the prior art known to those skilled in the art, and the model parameters of each electrical appliance are not specifically limited, and conventional equipment can be used.

[0067] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0068] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A method for producing a protein worm nutrition solid granule, characterized in that: The following steps are involved: Step 1: Prepare a certain amount of raw materials, including: selenium-enriched soybeans, dried protein worms, milk powder, probiotics, casein hydrolyzate, glucose, and sodium chloride; Step 2: Separately wash the selenium-rich soybeans and dried protein worms in an automatic washing machine, quickly spin dry, and send them to a low-temperature air-drying conveyor line to remove surface moisture, and then perform high-temperature dehydration and sterilization; Step 3: Send the processed selenium-enriched soybeans and dried protein worms into a grinder and grind them into powder; Step 4: adding soybean powder, sodium chloride, glucose and water by weight into a fermentation tank, sterilizing and then adding probiotics for fermentation and cultivation, concentrating the fermentation liquid by inorganic ceramic membrane microfiltration, and then drying by low-temperature fluidized bed to obtain concentrated crystals; Step 5, feeding the above-mentioned concentrated crystals, protein insect powder, milk powder and casein hydrolyzate into a pulverizer according to weight components, stirring and mixing to make powder, and sterilizing in an oven to obtain a powdered solid finished product; Step 6: Finally, the finished products are automatically vacuum-packed by an automatic packaging machine and stored.

2. A method for producing a protein worm nutrition solid granule according to claim 1, characterized in that: The weight percentages of the raw materials in step 1 are: 15-25% of selenium-enriched soybeans, 40-50% of dried protein worms, 15-25% of milk powder, 1-2% of probiotics, 7-9% of casein hydrolyzate, 1-3% of glucose and 3-5% of sodium chloride.

3. A method for producing a protein worm nutrition solid granule according to claim 1, characterized in that: In the step 2, the selenium-rich soybeans and dried protein worms are washed and then rapidly dehydrated, dried and sterilized at a high temperature of 100-400° C. in a large microwave oven.

4. A method for producing a protein worm nutrition solid granule according to claim 1, characterized in that: The sterilization temperature in step 4 is 121-126° C. and the time is 30-35 minutes.

5. A method for producing a protein worm nutrition solid granule according to claim 1, characterized in that: In the step 4, the fermentation culture temperature is 34-37° C., the initial pH is 7-8, the stirring speed is 120-140 r / min, the ratio of the volume of the fermentation liquid to the volume of the air introduced per minute is 1:0.4-0.6, and the fermentation time is 28-30 h.

6. A method for producing a protein worm nutrition solid granule according to claim 1, characterized in that: In the step 4, the pore size of the inorganic ceramic membrane tube is 0.2 μm, the concentration pressure is 0.15-0.18 MPa, and the membrane area is 0.25 m.

7. A method for producing a protein worm nutrition solid granule according to claim 1, characterized in that: In step 4, the drying air inlet temperature of the low-temperature fluidized bed is 40-60°C, the wind speed is 1.8-2.2 m / s, and the drying time is 40-70 min.

8. A method for producing a protein worm nutrition solid granule according to claim 1, characterized in that: In step 5, the temperature of the oven is 120-200° C. and the time is 30-90 minutes.

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