Moisturizing skin care product containing marine plant extract and preparation method thereof

Abstract

The invention discloses a moisturizing skin care product containing marine plant extracts and a preparation method of the moisturizing skin care product, the extracts are prepared from laminaria maximowiczii, eryngium maritimum, crithmum maritimum and laminaria saccharina through the steps of cleaning, microwave leaching, ultrasonic extraction, drying, supercritical extraction and the like, and the moisturizing skin care product has the characteristics of efficiently extracting and enriching active ingredients. In the preparation process, the marine plant extract with the water content lower than 5% is obtained by controlling parameters such as microwave, ultrasonic and supercritical extraction and optimizing extraction conditions. The extract can be used for preparing a moisturizing skin care product, and the skin care product further comprises a moisturizer, an emulsifier, a stabilizer, an antioxidant, a skin conditioner, a preservative, a pH regulator, essence and the like. The preparation method comprises the following steps: heating and dissolving, homogenizing, adding the marine plant extract, adjusting the pH value, cooling, adding the essence and the like. According to the test of the embodiment, the moisturizing skin care product provided by the invention has an excellent moisturizing effect and an excellent skin elasticity recovery effect.

Description

Technical Field

[0001] The invention belongs to the field of plant extracts, and specifically discloses a moisturizing skin care product containing a marine plant extract and a preparation method thereof. Background Art

[0002] With the improvement of people's living standards and the enhancement of aesthetic concepts, moisturizing skin care products have become an indispensable part of modern people's daily life. There are many kinds of moisturizing skin care products on the market, including products with marine plant extracts as the main ingredients. However, there are still some limitations that cannot be ignored in the application and development of marine plant extracts, especially in the selection of raw materials, product diversity and functionality.

[0003] On the one hand, the existing marine plant extracts are mostly limited to seaweed plants. Although seaweed plants are rich in various nutrients and active substances, and have moisturizing and anti-oxidation effects, the overly single raw material selection limits the diversity and innovation of products. Moisturizing skin care products on the market often have problems of product similarity and single functions, which makes it difficult to meet the growing personalized needs of consumers.

[0004] On the other hand, due to the limitations of raw material selection, existing marine plant extracts also have certain limitations in functionality. Although seaweed plants have a variety of biological activities, the functional differences between different seaweeds are not significant, resulting in a lack of breakthroughs and innovations in the functions of moisturizing skin care products on the market. In addition, some rare marine plant resources, such as Eryngium marinum and sea fennel, although they have unique biological activities and functions, have not yet been widely used due to their special growth environment and difficulty in collection, further limiting the expansion of marine plant extracts in the fields of moisturizing skin care products.

[0005] Therefore, how to break the limitations in raw material selection, broaden the sources of marine plant extracts, and how to achieve breakthroughs and innovations in functions have become important directions in current research on marine plant extracts. Summary of the invention

[0006] In view of the shortcomings of the prior art, the present invention proposes a moisturizing skin care product containing marine plant extracts and a preparation method thereof. By optimizing the extraction process and broadening the range of raw material selection, a more efficient and environmentally friendly preparation method of marine plant extracts is provided, and the method is applied to the preparation of moisturizing skin care products, in order to achieve product diversification and functional innovation.

[0007] The present invention includes the following technical solutions:

[0008] A marine plant extract is prepared by the following steps:

[0009] 1) Pretreatment: Select fresh large-leaf kelp, coastal celery, sea fennel, and sugar kelp, wash them, remove salt, sediment and other impurities attached to the surface, and drain the water;

[0010] 2) Microwave leaching: The cleaned marine plants are mechanically mixed, crushed and ground into marine plant slurry, which is then put into a container, and then an ethanol-water mixed solvent is added for leaching. The leaching temperature is 45-65° C. and the leaching time is 4-8 hours. Microwaves are used to assist the leaching to obtain an leaching solution;

[0011] 3) Ultrasonic extraction: the extract obtained in step 2) is placed in an ultrasonic device for ultrasonic-assisted extraction, with an ultrasonic frequency of 20-40 kHz, an ultrasonic time of 3-6 h, and a temperature of 45-55° C.

[0012] 4) Drying: subjecting the mixture after ultrasonic extraction to refrigerated centrifugal separation to remove solid residues, followed by reduced pressure concentration and spray drying to obtain marine plant extract powder with a water content of less than 5%;

[0013] 5) Supercritical extraction: The marine plant extract powder obtained in step 4) is further subjected to supercritical carbon dioxide SC-CO 2 Extraction is performed to enrich the active ingredients; the supercritical extraction conditions are: pressure 10-50MPa, temperature 30-60℃, extraction time 1-4h; after the extraction is completed, CO is released by decompression 2 , obtaining a marine plant supercritical extract, which is spray-dried again until the water content is less than 5% to obtain a marine plant extract.

[0014] Furthermore, in the above-mentioned marine plant extract, in the step 1), after draining the water, the extract is calculated by weight to include 100 parts of Laminaria japonica, 30-40 parts of Eryngium marinum, 15-25 parts of Cistanche deserticola, and 5-15 parts of Laminaria saccharina.

[0015] Furthermore, in the above-mentioned marine plant extract, in step 2), the microwave parameters are: frequency 2.45 GHz, power 400-1000 W, and container capacity 10-30 L.

[0016] Furthermore, in the above-mentioned marine plant extract, in the step 2), the volume ratio of the ethanol-water mixed solvent to the marine plant slurry is 2:1-2; in the ethanol-water mixed solvent, the volume ratio of ethanol to water is 3:1.

[0017] Furthermore, in the above-mentioned marine plant extract, in the 3) ultrasonic extraction, the capacity of the ultrasonic device is 20-40L, the ultrasonic power is 2000-3000W, and the ultrasonic cycle is 10s-stop 5s.

[0018] Furthermore, in the above-mentioned marine plant extract, in the 4) drying, the centrifugation parameter is 10000-15000g.

[0019] The invention also discloses the application of the marine plant extract in preparing moisturizing skin care products.

[0020] The present invention also discloses a moisturizing skin care product, which contains the following raw materials:

[0021] a) Marine plant extracts;

[0022] b) Moisturizer: one or more of hyaluronic acid, glycerol or trehalose;

[0023] c) Emulsifier: one or more of cetearyl alcohol, polysorbate 80, PEG-100 stearate or glyceryl stearate;

[0024] d) Stabilizer: one or more of disodium EDTA or carbomer;

[0025] e) Antioxidants: one or more of vitamin E, tea polyphenols or resveratrol;

[0026] f) Skin conditioning agents: one or more of squalane, allantoin or ceramide;

[0027] g) Preservatives: one or more of phenoxyethanol, potassium sorbate or sodium benzoate;

[0028] h) pH adjuster: one or more of citric acid, lactic acid or sodium hydroxide, to adjust the pH value of the product to 7.5-7.8;

[0029] i) Fragrance: one or more of lavender oil, sweet orange oil or tea tree oil;

[0030] j) Water, as solvent.

[0031] Furthermore, the above-mentioned moisturizing skin care product contains the following raw materials by weight:

[0032]

[0033] The present invention also discloses a method for preparing a moisturizing skin care product, comprising the following steps:

[0034] S1 Heating and dissolving: Add water to the reaction pot, heat to 70-80°C, then add moisturizer, emulsifier, stabilizer, antioxidant, skin conditioner and preservative in sequence, stirring while adding until completely dissolved;

[0035] S2 Homogenization: Use a homogenizer to homogenize the solution to improve the stability and fineness of the product;

[0036] S3: Add the marine plant extract: slowly add the prepared marine plant extract into the reaction pot and continue stirring to ensure uniform mixing;

[0037] S4 adjusts the pH value: adjusts the pH value of the solution to 7.5-7.8 using a pH adjuster;

[0038] S5 Cooling and adding essence: Cool the solution to below 40° C., then add the essence, stir evenly, filter and sterilize to obtain the moisturizing skin care product.

[0039] Compared with the prior art, the present invention has the following beneficial effects:

[0040] The invention discloses a light-weight heat-insulating high-strength building material and a preparation method thereof.

[0041] In terms of raw material selection, the present invention creatively combines four marine plants: Laminaria japonica, Eryngium marinum, Cercidiphyllum serrata and Laminaria saccharifolia. This combination not only enriches the raw material sources of marine plant extracts, but also achieves the complementarity and synergy of various plant active ingredients through scientific proportioning, thereby improving the overall biological activity of the extracts and the moisturizing and skin elasticity restoration effects. Compared with the traditional single or limited marine plant raw material selection, the raw material combination of the present invention is more diverse and innovative, providing a new idea and direction for the development of moisturizing skin care products.

[0042] Secondly, in terms of preparation technology, the present invention adopts a variety of modern extraction technologies such as microwave leaching, ultrasonic extraction and supercritical extraction, which effectively improves the extraction efficiency and purity of active ingredients in marine plants. These technologies not only optimize the extraction process and reduce the use of harmful solvents, but also retain the natural moisturizing factors and nutrients in the plants to the maximum extent, so that the final marine plant extract has higher quality and better moisturizing effect.

[0043] In addition, the moisturizing skin care product of the present invention has a scientific and reasonable formula, which not only contains highly effective marine plant extracts, but also adds a variety of moisturizers, emulsifiers, stabilizers, antioxidants, skin conditioners and preservatives to meet the needs of different skin types. By precisely controlling the proportion of each component and the preparation process, the moisturizing skin care product of the present invention has excellent stability and safety, can deeply nourish the skin, improve the skin's moisturizing ability and restore skin elasticity. BRIEF DESCRIPTION OF THE DRAWINGS

[0044] Figure 1 The skin elasticity test results of each group;

[0045] Figure 2 The test results of skin moisture content changes in each group are shown;

[0046] Figure 3Comparison results of the moisture growth rate (%) of each group from 0 to 28 days. DETAILED DESCRIPTION

[0047] A marine plant extract is prepared by the following steps:

[0048] 1) Pretreatment: Select fresh large-leaf kelp, coastal celery, sea fennel, and sugar kelp, wash them, remove salt, sediment and other impurities attached to the surface, and drain the water;

[0049] 2) Microwave leaching: The cleaned marine plants are mechanically mixed, crushed and ground into marine plant slurry, which is then put into a container, and then an ethanol-water mixed solvent is added for leaching. The leaching temperature is 45-65° C. and the leaching time is 4-8 hours. Microwaves are used to assist the leaching to obtain an leaching solution;

[0050] 3) Ultrasonic extraction: the extract obtained in step 2) is placed in an ultrasonic device for ultrasonic-assisted extraction, with an ultrasonic frequency of 20-40 kHz, an ultrasonic time of 3-6 h, and a temperature of 45-55° C.

[0051] 4) Drying: subjecting the mixture after ultrasonic extraction to refrigerated centrifugal separation to remove solid residues, followed by reduced pressure concentration and spray drying to obtain marine plant extract powder with a water content of less than 5%;

[0052] 5) Supercritical extraction: The marine plant extract powder obtained in step 4) is further subjected to supercritical carbon dioxide SC-CO 2 Extraction is performed to enrich the active ingredients; the supercritical extraction conditions are: pressure 10-50MPa, temperature 30-60℃, extraction time 1-4h; after the extraction is completed, CO is released by decompression 2 , obtaining a marine plant supercritical extract, which is spray-dried again until the water content is less than 5% to obtain a marine plant extract.

[0053] In the step 1), after draining the water, the following are calculated by weight: 100 parts of Laminaria japonica, 30-40 parts of Eryngium marinum, 15-25 parts of Fennel, and 5-15 parts of Laminaria saccharina.

[0054] In the step 2), the microwave parameters are: frequency 2.45 GHz, power 400-1000 W, and container capacity 10-30 L.

[0055] In the step 2), the volume ratio of the ethanol-water mixed solvent to the marine plant slurry is 2:1-2; in the ethanol-water mixed solvent, the volume ratio of ethanol to water is 3:1.

[0056] In the ultrasonic extraction described in 3), the capacity of the ultrasonic device is 20-40 L, the ultrasonic power is 2000-3000 W, and the ultrasonication is repeated for 10 s and 5 s.

[0057] In the drying step 4), the centrifugation parameter is 10000-15000 g.

[0058] The technical solutions in the embodiments of the present invention are described clearly and completely below. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0059] Example 1

[0060] A marine plant extract is prepared by the following steps:

[0061] 1) Pretreatment: Select fresh large-leaf kelp, coastal celery, sea fennel, and sugar kelp, wash them, remove salt, sediment and other impurities attached to the surface, and drain the water;

[0062] 2) Microwave leaching: The cleaned marine plants are mechanically mixed, crushed and ground into marine plant slurry, which is then put into a container, and then an ethanol-water mixed solvent is added for leaching. The leaching temperature is 45° C. and the leaching time is 8 h. Microwaves are used to assist the leaching to obtain an leaching solution;

[0063] 3) Ultrasonic extraction: the extract obtained in step 2) is placed in an ultrasonic device for ultrasonic-assisted extraction, with an ultrasonic frequency of 20 kHz, an ultrasonic time of 6 h, and a temperature controlled at 45°C;

[0064] 4) Drying: subjecting the mixture after ultrasonic extraction to refrigerated centrifugal separation to remove solid residues, followed by reduced pressure concentration and spray drying to obtain marine plant extract powder with a water content of less than 5%;

[0065] 5) Supercritical extraction: The marine plant extract powder obtained in step 4) is further subjected to supercritical carbon dioxide SC-CO 2 Extraction was performed to enrich the active ingredients; the supercritical extraction conditions were: pressure 10MPa, temperature 60°C, extraction time 1h; after the extraction, CO was released by decompression 2 , obtaining a marine plant supercritical extract, which is spray-dried again until the water content is less than 5% to obtain a marine plant extract.

[0066] In the step 1), after draining the water, the following are calculated by weight: 100 parts of Laminaria japonica, 30 parts of Eryngium marinum, 15 parts of Fennel, and 5 parts of Laminaria saccharina.

[0067] In the step 2), the microwave parameters are: frequency 2.45 GHz, power 400 W, and container capacity 10 L.

[0068] In the step 2), the volume ratio of the ethanol-water mixed solvent to the marine plant slurry is 2:1; in the ethanol-water mixed solvent, the volume ratio of ethanol to water is 3:1.

[0069] In the ultrasonic extraction described in 3), the capacity of the ultrasonic device is 20 L, the ultrasonic power is 2000 W, and the ultrasonic cycle is 10 s-off 5 s.

[0070] In the drying step 4), the centrifugation parameter is 10000 g.

[0071] Example 2

[0072] A marine plant extract is prepared by the following steps:

[0073] 1) Pretreatment: Select fresh large-leaf kelp, coastal celery, sea fennel, and sugar kelp, wash them, remove salt, sediment and other impurities attached to the surface, and drain the water;

[0074] 2) Microwave leaching: The cleaned marine plants are mechanically mixed, crushed and ground into marine plant slurry, which is then put into a container, and then an ethanol-water mixed solvent is added for leaching. The leaching temperature is 55° C. and the leaching time is 6 h. Microwaves are used to assist the leaching to obtain an leaching solution;

[0075] 3) Ultrasonic extraction: the extract obtained in step 2) is placed in an ultrasonic device for ultrasonic-assisted extraction, with an ultrasonic frequency of 30 kHz, an ultrasonic time of 4.5 h, and a temperature of 50° C.

[0076] 4) Drying: subjecting the mixture after ultrasonic extraction to refrigerated centrifugal separation to remove solid residues, followed by reduced pressure concentration and spray drying to obtain marine plant extract powder with a water content of less than 5%;

[0077] 5) Supercritical extraction: The marine plant extract powder obtained in step 4) is further subjected to supercritical carbon dioxide SC-CO 2 Extraction was performed to enrich the active ingredients; the supercritical extraction conditions were: pressure 30MPa, temperature 45°C, extraction time 2h; after the extraction, CO was released by decompression 2 , obtaining a marine plant supercritical extract, which is spray-dried again until the water content is less than 5% to obtain a marine plant extract.

[0078] In the step 1), after draining the water, the following are calculated by weight: 100 parts of Laminaria japonica, 35 parts of Eryngium marinum, 20 parts of Fennel, and 10 parts of Laminaria saccharina.

[0079] In the step 2), the microwave parameters are: frequency 2.45 GHz, power 700 W, and container capacity 20 L.

[0080] In the step 2), the volume ratio of the ethanol-water mixed solvent to the marine plant slurry is 2:1.5; in the ethanol-water mixed solvent, the volume ratio of ethanol to water is 3:1.

[0081] In the ultrasonic extraction described in 3), the capacity of the ultrasonic device is 30 L, the ultrasonic power is 2500 W, and the ultrasonic cycle is 10 s-off 5 s.

[0082] In the drying step 4), the centrifugation parameter is 12500 g.

[0083] Example 3

[0084] A marine plant extract is prepared by the following steps:

[0085] 1) Pretreatment: Select fresh large-leaf kelp, coastal celery, sea fennel, and sugar kelp, wash them, remove salt, sediment and other impurities attached to the surface, and drain the water;

[0086] 2) Microwave leaching: The cleaned marine plants are mechanically mixed, crushed and ground into marine plant slurry, which is then put into a container, and then an ethanol-water mixed solvent is added for leaching. The leaching temperature is 65° C. and the leaching time is 4 h. Microwaves are used to assist the leaching to obtain an leaching solution;

[0087] 3) Ultrasonic extraction: the extract obtained in step 2) is placed in an ultrasonic device for ultrasonic-assisted extraction, with an ultrasonic frequency of 40 kHz, an ultrasonic time of 3 h, and a temperature of 55° C.

[0088] 4) Drying: subjecting the mixture after ultrasonic extraction to refrigerated centrifugal separation to remove solid residues, followed by reduced pressure concentration and spray drying to obtain marine plant extract powder with a water content of less than 5%;

[0089] 5) Supercritical extraction: The marine plant extract powder obtained in step 4) is further subjected to supercritical carbon dioxide SC-CO 2 Extraction was performed to enrich the active ingredients; the supercritical extraction conditions were: pressure 50MPa, temperature 30°C, extraction time 4h; after the extraction, CO was released by decompression 2 , obtaining a marine plant supercritical extract, which is spray-dried again until the water content is less than 5% to obtain a marine plant extract.

[0090] In the step 1), after draining the water, the following are calculated by weight: 100 parts of Laminaria japonica, 40 parts of Eryngium marinum, 25 parts of Fennel, and 15 parts of Laminaria saccharina.

[0091] In the step 2), the microwave parameters are: frequency 2.45 GHz, power 1000 W, and container capacity 30 L.

[0092] In the step 2), the volume ratio of the ethanol-water mixed solvent to the marine plant slurry is 2:2; in the ethanol-water mixed solvent, the volume ratio of ethanol to water is 3:1.

[0093] In the ultrasonic extraction described in 3), the capacity of the ultrasonic device is 40 L, the ultrasonic power is 3000 W, and the ultrasonic cycle is 10 s-off 5 s.

[0094] In the drying step 4), the centrifugation parameter is 15000 g.

[0095] Comparative Example 1

[0096] A marine plant extract is prepared by the following steps:

[0097] 1) Pretreatment: Select fresh large-leaf kelp, coastal celery, sea fennel, and sugar kelp, wash them, remove salt, sediment and other impurities attached to the surface, and drain the water;

[0098] 2) Microwave leaching: The cleaned marine plants are mechanically mixed, crushed and ground into marine plant slurry, which is then put into a container, and then an ethanol-water mixed solvent is added for leaching. The leaching temperature is 55° C. and the leaching time is 6 h. Microwaves are used to assist the leaching to obtain an leaching solution;

[0099] 3) Ultrasonic extraction: the extract obtained in step 2) is placed in an ultrasonic device for ultrasonic-assisted extraction, with an ultrasonic frequency of 30 kHz, an ultrasonic time of 4.5 h, and a temperature of 50° C.

[0100] 4) Drying: subjecting the mixture after ultrasonic extraction to refrigerated centrifugal separation to remove solid residues, followed by reduced pressure concentration and spray drying to obtain marine plant extract powder with a water content of less than 5%;

[0101] 5) Supercritical extraction: The marine plant extract powder obtained in step 4) is further subjected to supercritical carbon dioxide SC-CO 2 Extraction was performed to enrich the active ingredients; the supercritical extraction conditions were: pressure 30MPa, temperature 45°C, extraction time 2h; after the extraction, CO was released by decompression 2 , obtaining a marine plant supercritical extract, which is spray-dried again until the water content is less than 5% to obtain a marine plant extract.

[0102] In the step 1), after draining the water, 100 parts of large-leaf kelp are added by weight.

[0103] In the step 2), the microwave parameters are: frequency 2.45 GHz, power 700 W, and container capacity 20 L.

[0104] In the step 2), the volume ratio of the ethanol-water mixed solvent to the marine plant slurry is 2:1.5; in the ethanol-water mixed solvent, the volume ratio of ethanol to water is 3:1.

[0105] In the ultrasonic extraction described in 3), the capacity of the ultrasonic device is 30 L, the ultrasonic power is 2500 W, and the ultrasonic cycle is 10 s-off 5 s.

[0106] In the drying step 4), the centrifugation parameter is 12500 g.

[0107] The raw material of this comparative example is large-leaf kelp, and does not contain coastal lycopodiella, sea fennel, and sugar kelp. The rest is the same as in Example 2.

[0108] Comparative Example 2

[0109] The raw materials of this comparative example are Laminaria japonica and Eryngium raphanii, and do not contain sea fennel and Laminaria saccharifolia. The rest is the same as in Example 2.

[0110] Comparative Example 3

[0111] The raw materials of this comparative example are Laminaria japonica, Eryngium marinum and Sea Fennel, and Laminaria saccharifolia is not contained. The rest is the same as in Example 2.

[0112] Comparative Example 4

[0113] The commercially available seaweed extract moisturizing skin care product is PROYA Watery Core Skin Lotion.

[0114] Preparation Example

[0115] According to the marine plant extracts of Examples 1-3 and Comparative Examples 1-3, corresponding moisturizing skin care products were prepared according to the following formula and preparation method.

[0116] A moisturizing skin care product, comprising the following raw materials by weight:

[0117]

[0118] The preparation method of the above-mentioned moisturizing skin care product comprises the following steps:

[0119] S1 Heating and dissolving: Add water to the reaction pot, heat to 70-80°C, then add moisturizer, emulsifier, stabilizer, antioxidant, skin conditioner and preservative in sequence, stirring while adding until completely dissolved;

[0120] S2 Homogenization: Use a homogenizer to homogenize the solution to improve the stability and fineness of the product;

[0121] S3: Add the marine plant extract: slowly add the prepared marine plant extract into the reaction pot and continue stirring to ensure uniform mixing;

[0122] S4 adjusts the pH value: adjusts the pH value of the solution to 7.5-7.8 using a pH adjuster;

[0123] S5 Cooling and adding essence: Cool the solution to below 40° C., then add the essence, stir evenly, filter and sterilize to obtain the moisturizing skin care product.

[0124] Test Case

[0125] The skin care products of Examples 1-3 and Comparative Examples 1-4 were subjected to actual performance evaluation.

[0126] 1. Safety testing,

[0127] The test method refers to the human skin patch test in the "2015 Cosmetic Safety Technical Specifications". The method of the skin occlusion patch test is: 80 volunteers aged 18-60 years old were selected and randomly divided into 4 groups, with an area of ​​no more than 50mm 2 , a qualified spot test device with a depth of about 1mm, take 0.020mL of sample and put it into the small chamber of the spot tester, the control well is a blank control (no substance is placed), the spot tester with skin care products is applied to the curved side of the forearm of the subject with a low-allergenic tape, and the palm is gently pressed to make it evenly applied to the skin for 24 hours. Observe the skin reaction according to the standards in Table 1 at 30 minutes, 24 hours and 48 hours after removing the spot tester, and record the observation results, such as the products of test examples 1-3, and the results are shown in Table 2.

[0128] Table 1 Skin reaction grading standard for skin occlusive patch test

[0129]

[0130] Table 2 Patch test results

[0131] Sample No. 30min 24h 48h result Example 1 0 0 0 No positive reaction Example 2 0 0 0 No positive reaction Example 3 0 0 0 No positive reaction Blank control 0 0 0 No positive reaction

[0132] It can be seen from the above table that the patch test results of the skin care products prepared in Examples 1-3 are all negative, indicating that the skin care products of the present invention are safe, non-toxic, mild and non-irritating.

[0133] 2. Skin elasticity test

[0134] A total of 240 volunteers were selected and divided into 8 groups with 30 people in each group in an environment with the same humidity. The average value was taken for the test. Examples 1-3, Comparative Examples 1-4 and a blank control (vaseline) were used respectively, with 2.0 μl / cm 2 Apply evenly on the back of your hand 3 times, once in the morning, noon and evening, and test the results on 0 days (before coating), 7 days and 14 days.

[0135] Skin elasticity test, using MPA580-Cutometer (2mm probe), elasticity index is R2: R2 value is considered to be the parameter value closest to the true state of skin elasticity, which is the ratio of the rebound displacement Ua to the maximum tensile displacement Uf, that is, the ratio of the total elastic-plasticity of the rebound part to the total elastic-plasticity of the tensile part. The closer the value is to 1, the better the skin elasticity is. The test results are shown in Table 3 and Figure 1 shown.

[0136] Table 3 Statistics of skin elasticity test

[0137] Group Day 0 (skin elasticity R2) 7 days (skin elasticity R2) 14 days (skin elasticity R2) Example 1 0.72 0.87 0.92 Example 2 0.70 0.89 0.94 Example 3 0.73 0.87 0.91 Comparative Example 1 0.69 0.75 0.81 Comparative Example 2 0.72 0.80 0.83 Comparative Example 3 0.70 0.84 0.87 Comparative Example 4 0.71 0.76 0.82 Blank control 0.72 0.74 0.78

[0138] From the data in Table 3, it can be seen that Examples 1-3 of the present invention have a good function of promoting skin elasticity recovery. From the comparison between Example 2 and Comparative Examples 1-3, it can be seen that the combination of marine plant extracts used in the present invention, i.e. 100 parts of Laminaria japonica, 35 parts of Eryngium marinum, 20 parts of Fennel, and 10 parts of Laminaria saccharina, has a significant synergistic effect of increasing skin elasticity. The average skin elasticity of 0.7 can be restored to 0.94 within 14 days. Compared with commercially available products, Comparative Example 4 using seaweed extract also has a significant improvement of more than 10%.

[0139] 3. Moisturizing effect test

[0140] 1) Experimental method: The principle of capacitance is mainly used, that is, the water content of the skin is measured based on the difference in dielectric constants between water and other substances. Under normal circumstances, the dielectric constant of water is 81, while the dielectric constants of other substances are mostly less than 7. In this way, the capacitor will change with the change of the capacitance of the skin, and the capacitance of the skin is within the measurement range, so it is easy to measure the water content of the skin.

[0141] 2) Test equipment

[0142] Skin Moisture Tester Corneometer CM825

[0143] Moisturizing test method: 200 female volunteers with an average age of 20 to 35 years old were selected and randomly divided into 5 groups, with 40 volunteers in each group, and the moisturizing skin care products in Example 2 and Comparative Examples 1-4 were used respectively.

[0144] The volunteers applied skin care products on their faces three times a day, morning, noon and evening, for two weeks.

[0145] The skin moisture content was tested with the Corneometer CM825 on the 0th day, 14th day and 28th day to evaluate the skin moisture content before and after using moisturizing skin care products.

[0146] The moisturizing effect test results are shown in Table 4 and Figure 2 and Figure 3 .

[0147] Table 4 Moisturizing performance test

[0148]

[0149] From the results in Table 4, it can be seen that Examples 1-3 of the present invention have a good function of promoting skin moisturizing. From the comparison between Example 2 and Comparative Examples 1-3, it can be seen that the combination of marine plant extracts used in the present invention, i.e., the combination of Laminaria japonica, Eryngium marinum, Cercidiphyllum saccharum, and Laminaria sacchari has an obvious synergistic effect of maintaining skin moisture. The moisture growth rate in 0-28 days can reach 38.5%. Compared with the commercially available products, Comparative Example 4 using seaweed extract also has a significant improvement of more than 20%.

[0150] From the above examples, it can be seen that the present invention creatively combines four marine plants, namely, Laminaria japonica, Eryngium marinum, Cercidiphyllum serrata and Laminaria saccharifolia. This combination not only enriches the raw material sources of marine plant extracts, but also achieves the complementarity and synergy of various plant active ingredients through scientific proportioning, thereby improving the overall biological activity of the extracts and the effects of moisturizing and promoting skin elasticity recovery. Compared with the traditional single or limited selection of marine plant raw materials, the raw material combination of the present invention is more diverse and innovative, providing a new idea and direction for the development of moisturizing skin care products.

[0151] The above are limited to several preferred embodiments of the present invention, and the description is relatively specific and detailed, but it cannot be understood as limiting the scope of the present invention. It should be pointed out that for ordinary technicians in this field, several modifications and improvements can be made without departing from the concept of the present invention, which all belong to the protection scope of the present invention.

Claims

1. A marine plant extract, characterized in that: Prepared by the following steps: 1) Pretreatment: Select fresh large-leaf kelp, coastal celery, sea fennel, and sugar kelp, wash them, remove salt, sediment and other impurities attached to the surface, and drain the water; 2) Microwave leaching: The cleaned marine plants are mechanically mixed, crushed and ground into marine plant slurry, which is then put into a container, and then an ethanol-water mixed solvent is added for leaching. The leaching temperature is 45-65° C. and the leaching time is 4-8 hours. Microwaves are used to assist the leaching to obtain an leaching solution; 3) Ultrasonic extraction: the extract obtained in step 2) is placed in an ultrasonic device for ultrasonic-assisted extraction, with an ultrasonic frequency of 20-40 kHz, an ultrasonic time of 3-6 h, and a temperature of 45-55° C.; 4) Drying: subjecting the mixture after ultrasonic extraction to refrigerated centrifugal separation to remove solid residues, followed by reduced pressure concentration and spray drying to obtain marine plant extract powder with a water content of less than 5%; 5) Supercritical extraction: The marine plant extract powder obtained in step 4) is further extracted by supercritical carbon dioxide SC-CO2 to enrich the active ingredients; the supercritical extraction conditions are: pressure 10-50MPa, temperature 30-60°C, extraction time 1-4h; after the extraction, CO2 is released by decompression to obtain a marine plant supercritical extract, which is spray-dried again until the water content is less than 5% to obtain a marine plant extract.

2. A marine plant extract according to claim 1, characterized in that: In the step 1), after draining the water, the following are calculated by weight: 100 parts of Laminaria japonica, 30-40 parts of Eryngium marinum, 15-25 parts of Fennel, and 5-15 parts of Laminaria saccharina.

3. A marine plant extract according to claim 1, characterized in that: In the step 2), the microwave parameters are: frequency 2.45 GHz, power 400-1000 W, and container capacity 10-30 L.

4. A marine plant extract according to claim 1, characterized in that: In the step 2), the volume ratio of the ethanol-water mixed solvent to the marine plant slurry is 2:1-2; in the ethanol-water mixed solvent, the volume ratio of ethanol to water is 3:

1.

5. A marine plant extract according to claim 1, characterized in that: In the ultrasonic extraction described in 3), the capacity of the ultrasonic device is 20-40 L, the ultrasonic power is 2000-3000 W, and the ultrasonication is repeated for 10 s and 5 s.

6. A marine plant extract according to claim 1, characterized in that: In the drying step 4), the centrifugation parameter is 10000-15000 g.

7. Use of the marine plant extract according to any one of claims 1 to 6 in the preparation of moisturizing skin care products.

8. A moisturizing skin care product, characterized in that: Contains the following ingredients: a) A marine plant extract prepared according to any one of claims 1 to 7; b) Moisturizer: one or more of hyaluronic acid, glycerol or trehalose; c) Emulsifier: one or more of cetearyl alcohol, polysorbate 80, PEG-100 stearate or glyceryl stearate; d) Stabilizer: one or more of disodium EDTA or carbomer; e) Antioxidants: one or more of vitamin E, tea polyphenols or resveratrol; f) Skin conditioning agents: one or more of squalane, allantoin or ceramide; g) Preservatives: one or more of phenoxyethanol, potassium sorbate or sodium benzoate; h) pH adjuster: one or more of citric acid, lactic acid or sodium hydroxide, to adjust the pH value of the product to 7.5-7.8; i) Fragrance: one or more of lavender oil, sweet orange oil or tea tree oil; j) Water, as solvent.

9. A moisturizing skin care product, characterized in that: By weight, it contains the following raw materials:

10. A method for preparing a moisturizing skin care product according to claim 8 or 9, characterized in that: The following steps are involved: S1 Heating and dissolving: Add water to the reaction pot, heat to 70-80°C, then add moisturizer, emulsifier, stabilizer, antioxidant, skin conditioner and preservative in sequence, stirring while adding until completely dissolved; S2 Homogenization: Use a homogenizer to homogenize the solution to improve the stability and fineness of the product; S3: Add the marine plant extract: slowly add the prepared marine plant extract into the reaction pot and continue stirring to ensure uniform mixing; S4 adjusts the pH value: adjusts the pH value of the solution to 7.5-7.8 using a pH adjuster; S5 Cooling and adding essence: Cool the solution to below 40° C., then add the essence, stir evenly, filter and sterilize to obtain the moisturizing skin care product.

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