Application of akebia stem extract in cosmetics

Abstract

According to the application of the akebia stem extract in skin oil control and / or hair loss prevention, the akebia stem extract is prepared through a solvent extraction method, and skin oil control and / or hair loss prevention are / is achieved by inhibiting 5 alpha-reductase. The invention also provides application of the akebia stem extract in preparation of a skin external preparation with skin oil control and / or hair loss prevention effects.

Description

Technical Field

[0001] The invention belongs to the field of phytochemistry, and particularly relates to an Akebia extract and application thereof in a skin external preparation or cosmetics. Background Art

[0002] Androgenic alopecia (AGA) is mainly manifested by the shortening of the growth phase of hair follicles and the miniaturization of terminal hair follicles. Experiments have shown that androgens play a vital role in the pathogenesis of AGA. Testosterone is the main androgen in the body. It is converted into dihydrotestosterone (DHT) by 5-α reductase. The latter has an affinity for androgen receptors that is 5 times that of testosterone, which can cause the transformation of terminal hair to vellus hair and eventually lead to hair loss. Current treatments include drug therapy and non-drug therapy, such as 5-α reductase inhibitors, platelet-rich plasma and hair transplantation.

[0003] 5-α reductase inhibitors can effectively inhibit the conversion of testosterone to DHT, thereby reducing the DHT in the scalp in the hair loss area and promoting hair growth.

[0004] Wu Chuanling et al. established an in vitro 5-α reductase screening model and proved that lotus seed core extract has 5-α reductase inhibitory activity; Deng Guiqiu et al. established an in vitro model for inhibiting 5-α reductase by high performance liquid chromatography, and the study proved that safflower extract has a certain inhibitory effect on type I and type II 5-α reductase; Zhao Qi et al. determined the 5-α reductase inhibitory activity of broad-leaved urtica extract by high performance liquid chromatography, and the study showed that the n-butanol part of the water extract of broad-leaved urtica whole herb has significant 5-α reductase inhibitory activity; Kazuya et al. established an in vitro 5-α reductase activity determination model and found that pepper, schisandra chinensis, kudzu flower, ginsenosides, etc. have certain inhibitory activity against 5-α reductase; Zhang Bei et al. established an in vitro enzyme activity system and screened out Platycladus orientalis leaf extract from 9 kinds of Chinese medicines for tonifying the kidney and liver, benefiting the essence and blood, and cooling the blood and promoting blood circulation, and found that it has better 5-α reductase inhibitory activity. Fu Shiyu et al. screened out effective monomers with inhibitory activity, including total glycosides of Anemarrhena asphodeloides, Forsythiae and Lupulone, through the 5-α reductase in vitro activity screening system; Yang Xiaoqing et al. studied the 5-α reductase inhibitory activity of neferine and isoliensinine, and the results showed that both had strong inhibitory activity and had application prospects; Ju Qiang et al. studied the effects of berberine hydrochloride and baicalin on the expression of 5-α reductase, and found that berberine hydrochloride may antagonize the stimulating effect of dihydrotestosterone on the activity of human sebaceous gland cells by inhibiting the expression of 5-α reductase.

[0005] Akebia is the dried vine of Akebia quinata (Thunb.) Decne, Akebia trifoliata (Thunb.) Koidz. or Akebia trifoliata (Thunb.) Koidz. var. australis (Diels) Rehd. of the Akebiaceae family.

[0006] KR100816264 discloses a cosmetic composition for abirritating skin containing extract of Akebiaquinata as active ingredient. The patent reports that Akebia has the effect of relieving irritation.

[0007] KR20150045420 discloses a composition for blocking AGEs production comprising Akebia quinata extracts. The patent reports the anti-glycation effect of Akebia quinata, which can be used in anti-aging products.

[0008] KR102036826 discloses a composition for preventing or treating atopic dermatitis comprising Akebia quinata extracts or fractions thereof as an active ingredient.

[0009] KR20140071716 discloses a cosmetic composition comprising the extract of akebia quinata as an active ingredient. The patent reports that akebia quinata has anti-inflammatory, whitening and moisturizing effects.

[0010] KR101427462 discloses a composite natural antiseptic from Forsythia suspensa Vahl, Leonurus sibiricus L., Crataegi fructus and Akebia quinata Decaisne, Cosmetic Compositions Containing Them. The patent reports that the Akebia composition has antibacterial and antiseptic effects.

[0011] CN201711486685.5 discloses a Chinese herbal medicine composition for treating hair loss. The patent reports a Chinese herbal medicine composition containing Akebia.

[0012] However, none of the prior art documents have found the use of Akebia as a skin oil control and / or anti-hair loss ingredient in cosmetics. Summary of the invention

[0013] In one aspect, the present invention provides the use of an Akebia extract in controlling skin oiliness and / or preventing hair loss, wherein the Akebia extract is prepared by a solvent extraction method.

[0014] In a preferred embodiment, the Akebia extract is extracted using water as a solvent and is prepared by decoction extraction.

[0015] In a preferred embodiment, the Akebia extract is used at a concentration of 0.001 to 1 mg / mL, more preferably 0.007 mg / mL-0.5 mg / mL.

[0016] In a preferred embodiment, the skin oil control and / or hair loss prevention is achieved by inhibiting 5α-reductase.

[0017] On the other hand, the present invention also relates to the use of the Akebia extract in the preparation of a skin external preparation with the effects of skin oil control and / or hair loss prevention, wherein the Akebia extract is prepared by a solvent extraction method.

[0018] In a preferred embodiment, the skin oil control and / or hair loss prevention is achieved by inhibiting 5α-reductase.

[0019] In a preferred embodiment, the weight percentage of the Akebia extract in the external skin preparation is 0.0005-10 weight %, preferably 0.000731-5 weight %, more preferably 0.003655-1 weight %, and most preferably 0.00731-0.5 weight %.

[0020] In a preferred embodiment, the skin external preparation is selected from the group consisting of: facial cream, lotion, gel, toner, essence, facial mask, eye cream, aerosol cleansing foam, spray, shower gel, and facial cleanser.

[0021] BRIEF DESCRIPTION OF THE DRAWINGS

[0022] Figure 1 The chromatographic results for a testosterone standard are shown.

[0023] Figure 2 The linear equation for the testosterone determination is shown. DETAILED DESCRIPTION

[0024] The present invention finds that the Akebia extract has the function of inhibiting 5-α reductase and can be added into skin care products as a single functional additive to assist in alleviating scalp, preventing hair loss and other related problems.

[0025] In order to provide a more concise description, some quantitative expressions given herein are not modified by the term "about". It should be understood that, whether or not the term "about" is explicitly used, each quantity given herein is intended to refer to the actual given value, and is also intended to refer to the approximate values ​​of these given values ​​that can be reasonably inferred by ordinary technicians in the field, including the approximate values ​​of these given values ​​caused by experimental and / or measurement conditions.

[0026] To provide a more concise description, some quantitative expressions herein are described as a range of about X amount to about Y amount. It should be understood that when describing a range, the range is not limited to the upper and lower limits described, but should include the entire range of about X amount to about Y amount or any amount therebetween.

[0027] Akebia Extract

[0028] Akebia is the dried vine of Akebia quinata (Thunb.) Decne, Akebia trifoliata (Thunb.) Koidz. or Akebia trifoliata (Thunb.) Koidz. var. australis (Diels) Rehd. of the Akebiaceae family.

[0029] The present invention firstly finds that the extract of Akebia has the effect of inhibiting 5-α reductase, and the single herb of Akebia has the effect of controlling skin oil and / or preventing hair loss. Therefore, the extract of Akebia can be added to skin care products as a single functional additive to help alleviate scalp, prevent hair loss and other related problems.

[0030] Specifically, the present invention finds that the Akebia extract has a 5α-reductase inhibitory effect. Therefore, the Akebia extract of the present invention can assist in relieving scalp, has skin oil control and / or anti-hair loss effects, and can be added to skin topical preparations as an efficacy additive for skin oil control and / or anti-hair loss.

[0031] 5α-reductase is an important enzyme in the androgen metabolism process. This enzyme can convert testosterone into more active dihydrotestosterone. It is widely involved in the metabolism of androgens in the body and plays an important physiological role. Abnormal expression of 5α-reductase in the body can lead to abnormal hormone levels in local tissues or throughout the body, thus leading to the occurrence of various diseases, such as acne and androgenic alopecia.

[0032] In sebaceous gland cells, androgens can be converted into more active dihydrotestosterone under the action of 5α-reductase, further stimulating sebum secretion, thereby enhancing the ability to induce acne. Therefore, inhibiting the activity of 5α-reductase can also control the secretion of skin oil and effectively relieve the occurrence of acne.

[0033] In addition, hormonal alopecia is positively correlated with the content of local androgens, especially dihydrotestosterone, and 5α-reductase is the key enzyme for converting testosterone into dihydrotestosterone. Therefore, inhibiting the activity of 5α-reductase can effectively alleviate seborrheic alopecia.

[0034] In some embodiments, the Akebia is extracted by heat extraction. For example, the decoction extraction method can be used for extraction. In some embodiments, the extraction solvent is water (e.g., deionized water). In some embodiments, the extraction process includes an alcohol precipitation step. For example, 95% ethanol is used to precipitate to a final concentration of 70%. In some embodiments, the extraction process includes a filtering step. In some embodiments, the extraction process also includes a concentration step. For example, concentrated to a solid content of 5-10% by weight.

[0035] In some embodiments, the Akebia extract is used at a concentration of 0.001 mg / mL to 1 mg / mL. A preferred concentration is 0.007 mg / mL to 1 mg / mL. A more preferred concentration is 0.007 mg / mL to 0.5 mg / mL. A more preferred concentration is 0.007 mg / mL to 0.1 mg / mL. A most preferred concentration is 0.00731 mg / mL to 0.0731 mg / mL.

[0036] Skin topical preparation containing Akebia extract

[0037] The Akebia extract of the present invention can be used as an effective additive in a skin topical preparation. In some embodiments, the skin topical preparation is selected from: facial cleanser, lotion, emulsion, cream, gel, and facial mask. Different dosages are added according to different types of preparations.

[0038] On the other hand, a skin external preparation is provided, which comprises the Akebia extract prepared according to the method of the present invention and an excipient acceptable in the field of cosmetics.

[0039] The skin external preparation is a general concept for all ingredients usually used on the outside of the skin, for example, a cosmetic composition or a pharmaceutical composition. The cosmetic composition may be a basic cosmetic, a facial makeup cosmetic, a body cosmetic, a hair care cosmetic, etc., and there is no particular restriction on its dosage form, which can be reasonably selected according to different purposes. The cosmetic composition also contains different media or matrix excipients permitted by the cosmetics level according to different dosage forms and purposes.

[0040] The cosmetic, dermatological or pharmaceutically acceptable excipients that can be used for the skin topical composition of the present invention are in the form of an aqueous phase, an oil phase, a gel, a wax-in-water emulsion, an oil-in-water emulsion or an oil-in-water emulsion. The aqueous phase is a mixture of one or more water-soluble or dispersible components, which can be liquid, semi-solid or solid at room temperature (25° C.). The excipient includes or can be in the form of a suspension, dispersion or solution in water or a water-alcohol excipient, which can contain a thickener or a gelling agent. Those skilled in the art can select a suitable product form based on the knowledge mastered by those skilled in the art, the components contained therein.

[0041] The composition may include an aqueous phase, which may contain water or a mixture of water and at least one hydrophilic organic solvent, such as an alcohol, especially a linear or branched lower monohydric alcohol containing 2 to 5 carbon atoms, such as ethanol or propanol; a polyol, such as propylene glycol, sorbitol, glycerol, panthenol or polyethylene glycol, and mixtures thereof.

[0042] When the composition of the invention is in the form of an emulsion, the composition may also optionally contain a surfactant.

[0043] The composition may also comprise a film-forming polymer, such as polyurethanes, polyacrylic acid homopolymers or copolymers, polyesters, hydrocarbon-based resins and / or silicone resins. The polymer may be dissolved or dispersed in a cosmetically acceptable vehicle and optionally combined with a plasticizer.

[0044] The composition of the present invention may also comprise an oil phase, which contains an oil-soluble or oil-dispersible component that is liquid at room temperature (25° C.) and / or an oily or waxy substance at room temperature, such as wax, semisolid, gum and a mixture thereof. The oil phase may also contain an organic solvent.

[0045] Suitable oily substances are usually liquid at room temperature and include: hydrocarbon-based oils of animal origin, such as fully hydrogenated squalene; hydrocarbon-based vegetable oils, such as liquid C4-10 fatty acid triglycerides, for example heptanoic acid or caprylic acid triglycerides, or oils, for example sunflower oil, corn oil, soybean oil, grape seed oil, castor oil, avocado oil, caprylic / capric triglycerides, jojoba oil; linear or branched hydrocarbons of mineral or synthetic origin, for example liquid paraffin and its derivatives, vaseline; synthetic esters and ethers, in particular esters of fatty alcohols, for example isopropyl myristate, 2-ethylhexyl palmitate, 2-octyldodecyl stearate, isostearyl isostearate; hydroxylated esters, for example isostearyl lactate, hydroxy octyl stearate, octyl hydroxystearate, octyldodecyl hydroxystearate, heptanoates, caprylates and caprates of fatty alcohols; polyol esters, such as propylene glycol dicaprylate, neopentyl glycol diheptanoate, diethylene glycol diisononanoate and pentaerythritol esters; fatty alcohols containing C12-26, such as octyldodecanol, 2-butyloctanol, 2-hexyldecanol, 2-undecylpentadecanol, oleyl alcohol; fluorinated oils and / or fluorinated silicone oils based on partial hydrocarbons, silicone oils, volatile or non-volatile linear or cyclic polymethylsiloxanes that are liquid or semisolid at room temperature, such as cyclopolydimethylsiloxanes and polydimethylsiloxanes, which optionally contain phenyl groups, such as phenyltrimethicone, siloxanes and mixtures thereof.

[0046] The composition of the present invention may further comprise any components commonly used in the cosmetic field. These components include preservatives, aqueous phase thickeners (extract biopolymers, synthetic polymers) and fat phase thickeners, fragrances, hydrophilic and lipophilic active agents and mixtures thereof.

[0047] The composition of the present invention may also contain an additional particulate phase, which may be a pigment and / or pearlescent agent and / or filler used in cosmetic compositions.

[0048] Pigments may be present in the composition, suitable inorganic pigments include titanium, zirconium and cerium oxides as well as zinc oxide, iron oxide and ferric blue; suitable organic pigments include barium, strontium, calcium and aluminium lakes and carbon black.

[0049] Pearlescent agents may be present in the composition. Suitable pearlescent agents include mica coated with titanium oxide, iron oxide or natural pigments.

[0050] Fillers may be present in the composition, suitable fillers include talc, silica, zinc stearate, mica, kaolin, nylon powder, polyethylene powder, Teflon, starch, boron nitride, copolymer microspheres, such as silicone resin microspheres.

[0051] The oil phase of the composition of the present invention may comprise one or more waxes, gums or mixtures thereof. Waxes include hydrocarbon-based waxes, fluoro waxes and / or silicone waxes and may be derived from plant, mineral, animal and / or synthetic sources. Suitable waxes include beeswax, carnauba wax, candelilla wax, paraffin wax, microcrystalline wax, ozokerite wax; synthetic waxes include polyethylene wax, silicone wax containing C16-45. Gums are generally polydimethylsiloxane or sodium carboxymethylcellulose or extracts, and semisolid substances are generally hydrocarbon-based compounds, such as lanolin and its derivatives.

[0052] The composition of the present invention can be formulated into any suitable product form. Such product forms include, but are not limited to, aerosol sprays, creams, emulsions, solids, liquids, dispersions, foams, gels, lotions, mousses, ointments, powders, patches, pomades, solutions, hand-pump sprays, sticks, facial masks, and wet wipes. The composition of the present invention can be conveniently used to prepare or as a cosmetic, dermatological, or pharmaceutical topical application product by various methods well known in the art.

[0053] The skin topical composition of the present invention may include one or more of the following ingredients: antiallergic agents, antimicrobial agents, antioxidants, chelating agents, colorants, depigmenting agents, emollients, emulsifiers, exfoliants, film formers, fragrances, moisturizers, insect repellents, lubricants, pharmaceutically active agents, moisturizers, photostabilizers, preservatives, skin care agents, skin penetration enhancers, sunscreens, stabilizers, surfactants, thickeners, viscosity regulators, vitamins or any combination thereof.

[0054] The present invention will be further described below in conjunction with specific examples. However, it should be understood that these examples are only used to illustrate the present invention and do not constitute a limitation of the scope of the present invention. The test methods in the following examples that do not specify specific conditions are usually based on conventional conditions or the conditions recommended by the manufacturer. Unless otherwise stated, all percentages and parts are by weight.

[0055] The experimental materials and equipment used in the following examples are as follows:

[0056]

[0057] Example 1: Preparation of Akebia extract

[0058] Weigh 100g of Akebia, extract twice with 10 times deionized water, filter, decoct and concentrate, add a certain amount of 95% ethanol to precipitate (final concentration 70%). Filter with filter paper, concentrate the filtrate at 55°C, and let stand overnight at 4°C. Take out and centrifuge at 3°C ​​3500rpm for 15min, filter with filter paper, and finally prepare a solution with a solid content of 7.31%, i.e. 0.731mg / mL, for use.

[0059] Example 2: Preparation of Akebia extract

[0060] The Akebia extract sample with a solid content of 7.31% prepared in Example 1 was added with deionized water to prepare a 5% solid content solution, i.e., 0.5 mg / mL, for later use.

[0061] Example 3: Preparation of Akebia extract

[0062] The Akebia extract sample with a solid content of 7.31% prepared in Example 1 was added with deionized water to prepare a solution with a solid content of 4%, i.e., 0.4 mg / mL, for later use.

[0063] Example 4: Preparation of Akebia extract

[0064] The Akebia extract sample with a solid content of 7.31% prepared in Example 1 was added with deionized water to prepare a 1% solid content solution, i.e., 0.1 mg / mL, for later use.

[0065] Example 5: Preparation of Akebia extract

[0066] The Akebia extract sample with a solid content of 7.31% prepared in Example 1 was added with deionized water to prepare a solution with a solid content of 0.731%, i.e., 0.0731 mg / mL, for later use.

[0067] Example 6: Evaluation of 5-α-reductase activity inhibition

[0068] Testosterone will be converted into more active dihydrotestosterone (DHT) by 5α-reductase with the participation of NADPH. Therefore, the inhibitory effect of the test substance on 5α-reductase can be measured by measuring the change in the testosterone content in the reaction system.

[0069] 1. Experimental Methods

[0070] (1) Preparation of 5-α-reducing crude enzyme extract (RLM): Take male SD rats, decapitate and bleed, take the liver and place it in an ice bath beaker, rinse it three times with ice bath physiological saline to wash away the residual blood. Cut it into pieces with scissors, add 3 times the weight-volume amount of phosphate (K2HPO4-KH2PO4, 100mM, pH7.4)-KCl (0.15M) buffer (pH7.4), and homogenize it mechanically with a homogenizer in an ice bath. Centrifuge the homogenate at 9000×g and 4℃ for 20min, take the supernatant and centrifuge it at 100000×g and 4℃ for 60min, and discard the supernatant. Add an equal amount of phosphate buffer to the supernatant, and blow it repeatedly with a pipette to disperse the precipitate. Centrifuge it again at 100000×g and 4℃ for 60min, and discard the supernatant again. Add phosphate buffer and resuspend it with a pipette to obtain a 5-α-reducing crude enzyme solution. The protein concentration was determined by the Bardford method (with BSA as the standard), diluted to a protein concentration of 10 mg / mL, and finally stored in a -80°C refrigerator.

[0071] (2) Enzymatic reaction: The reaction components were added to a 96-well plate in the order of buffer (Tris-HCl, pH 7.4), RLM (10 mg / mL), samples of different concentrations, dutasteride, testosterone (6 μg / mL), and NADPH (10 mM). The reaction system was added according to the table below. The reaction temperature was 37°C, the reaction time was 30 min, and the total volume of the reaction system was 100 μL. After the reaction, 50 μL of the reaction solution was taken and 100 μL of methanol was added to terminate the reaction. The plate was centrifuged at 10,000 × g for 10 min, and the supernatant was taken for testing. Three replicate wells were set up for each group.

[0072] Table 1

[0073]

[0074] Note: “ / ” means no addition

[0075] (3) Sample preparation: Prepare the solution according to the table below.

[0076] Table 2

[0077]

[0078] (3) Quantitative determination of testosterone: The UPLC-MS method is used to quantitatively determine the testosterone content in the sample to be tested, and the consumption of the substrate testosterone reflects the strength of the enzyme activity.

[0079] Chromatographic conditions: Waters Acquity HSS T3 column (100×2.1 mm, 1.8 μm)

[0080] Mobile phase: 0.1% formic acid in water: acetonitrile

[0081] Elution: Gradient elution as shown in the table below

[0082] Table 3

[0083]

[0084]

[0085] Flow rate: 0.4mL / min

[0086] Injection volume: 5 μL

[0087] Column temperature: 40°C

[0088] The mass spectrometer was analyzed in the positive ion mode of the electrospray ion source, and the data were collected in the full scan-single ion monitoring (Fullscan-SIM) mode, with a range of 100-1500 m / z and a resolution of 70000; the spray voltage was 3.5 kV; the capillary temperature was 320°C; the heating temperature was 300°C; the sheath gas flow rate was 30 Arb; the auxiliary gas flow rate was 13 Arb; and the automatic gain control (AGC) was 10 6 .

[0089] When the testosterone consumption in the sample tube is lower than the testosterone consumption in the reaction tube without inhibitor, it is considered to have inhibited 5-α reductase activity.

[0090]

[0091] Among them, B is the testosterone content of the blank control group, R is the testosterone content of the inhibitor reaction group, and Q is the testosterone content of the non-inhibitor reaction group.

[0092] 2 Experimental results:

[0093] (1) Linear equation of testosterone standard

[0094] Figure 1 The chromatographic results for a testosterone standard are shown.

[0095] Table 4

[0096]

[0097] Figure 2 The linear equation for the testosterone determination is shown.

[0098] The standard curve is Y = 653053.4313X-2356631.8540R 2 =0.9962, and the testosterone concentration showed a good linear dose-effect relationship in the mass concentration range of 6.25-200 ng / mL.

[0099] (2) 5-α-reductase inhibitory activity in the sample to be tested

[0100] Table 5

[0101]

[0102] From the results of the 5-α reductase inhibition experiment, it can be seen that the extract of Example 1 as a single herb has a certain ability to inhibit 5-α reductase when the mass concentration is 0.00731 mg / mL-0.0731 mg / mL.

[0103] The preparations of Examples 1 to 5 are used for the preparation of external skin preparations. The external skin preparations are preferably cosmetic compositions, such as lotions, essences, creams, etc. The weight percentage of the preparations in the external skin preparations is 0.0005%-10% (w / w). The preferred weight percentage is 0.000731%-5% (w / w). The more preferred weight percentage is 0.003655%-1% (w / w). The most preferred weight percentage is 0.00731%-0.5% (w / w).

[0104] The following are examples of specific applications of Akebia extract in skin external preparations, as well as the formulations and preparation methods of these preparations. In the following tables, "-" means no addition.

[0105] Example 7: Preparation of facial cream

[0106]

[0107]

[0108] Example 8: Preparation of emulsion

[0109]

[0110] Example 9: Preparation of gel

[0111]

[0112] Example 10: Preparation of lotion

[0113]

[0114] Example 11: Preparation of Essence

[0115]

[0116] Example 12: Preparation of facial mask

[0117]

[0118]

[0119] Example 13: Preparation of eye cream

[0120]

[0121] Example 14: Preparation of Aerosol (Cleansing Bubble)

[0122]

[0123] Example 15: Preparation of spray

[0124]

[0125] Example 16: Preparation of shower gel

[0126]

[0127] Example 17: Preparation of facial cleanser

[0128]

[0129]

Claims

1. Application of Akebia extract in skin oil control and / or hair loss prevention, wherein: The Akebia extract is prepared by a solvent extraction method.

2. The use according to claim 1, wherein: The Akebia extract is extracted using water as a solvent and is prepared by decoction extraction.

3. The use according to claim 1, wherein: The Akebia extract is used at a concentration of 0.001 to 1 mg / mL.

4. The use according to claim 1, wherein: The use concentration of the Akebia extract is 0.007 mg / mL-0.5 mg / mL.

5. The use according to any one of claims 1 to 4, wherein: The skin oil control and / or hair loss prevention are achieved by inhibiting 5α-reductase.

6. Use of Akebia extract in the preparation of a skin topical preparation having the functions of skin oil control and / or hair loss prevention, wherein: The Akebia extract is prepared by a solvent extraction method.

7. The use according to claim 6, wherein: The Akebia extract is extracted using water as a solvent and is prepared by decoction extraction.

8. The use according to claim 6, wherein: The skin oil control and / or hair loss prevention are achieved by inhibiting 5α-reductase.

9. The use according to claim 6, wherein: The weight percentage of the Akebia extract in the external skin preparation is 0.0005-10 weight %, preferably 0.000731-5 weight %, more preferably 0.003655-1 weight %, and most preferably 0.00731-0.5 weight %.

10. The use according to any one of claims 6 to 9, characterized in that: The skin external preparation is selected from: facial cream, lotion, gel, toner, essence, facial mask, eye cream, aerosol cleansing foam, spray, shower gel, facial cleanser.

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