Artemisia annua fat-soluble extract as well as preparation method and application thereof

Abstract

The invention relates to the technical field of extraction of fat-soluble components in artemisia annua, and particularly discloses an artemisia annua fat-soluble extract as well as a preparation method and application thereof. The invention relates to a preparation method of an artemisia annua fat-soluble extract. The preparation method comprises the following steps: 1, preparing artemisia annua powder from artemisia annua leaves; step 2, soaking the artemisia annua powder into a composite solvent formed by mixing ethanol, normal hexane, petroleum ether, succinic acid and pentanediol, extracting, and collecting an extracting solution; step 3, repeating the operation in the step 2 for 2-3 times, and combining extract liquor to obtain a primary extract of the artemisia annua; and step 4, dewaxing to obtain the artemisia annua extract. According to the method, a composite solvent system is adopted, fat-soluble components including sesquiterpene lactone and flavonoid active substances in artemisia annua can be comprehensively extracted, and loss of the active components in a traditional method is effectively avoided.

Description

Technical Field

[0001] The invention relates to the technical field of extraction of fat-soluble components in Artemisia annua, and in particular to an Artemisia annua fat-soluble extract, a preparation method and application thereof. Background Art

[0002] Artemisia annua Linn. is an annual herbaceous plant of the genus Artemisia in the Asteraceae family. At the same time, Artemisia annua Linn., as a traditional Chinese medicinal material, has long been recorded in the Chinese Pharmacopoeia. It is bitter and cold in nature and has multiple effects such as clearing away heat, relieving summer heat, relieving malaria, jaundice, and cooling blood. Artemisia annua Linn. contains rich nutrients, mainly including volatile oils, artemisinin, flavonoid compounds, etc. After the development of the artemisinin industry in China, there are professionally planted high-yield and high-quality Artemisia annua raw materials. However, in the artemisinin industry, only artemisinin in Artemisia annua Linn. is extracted, and other sesquiterpene lactones and flavonoid active substances are mostly discarded. According to the literature, these discarded active substances all have good anti-inflammatory and oil-control effects. Therefore, it is a technical problem to be solved urgently by those skilled in the art to develop an extraction method that can extract all fat-soluble components in Artemisia annua Linn. At the same time, it is a technical problem to be solved by those skilled in the art to keep the active components of the extract from being destroyed and to improve the bioavailability of the fat-soluble extract of Artemisia annua Linn. Summary of the invention

[0003] In order to develop an extraction method that can extract all fat-soluble components in Artemisia annua at the same time, thereby improving the bioavailability of Artemisia annua fat-soluble extracts in the cosmetics field, the present application provides an Artemisia annua fat-soluble extract and its preparation method and application. The present application adopts a composite solvent system, which can comprehensively extract the fat-soluble components in Artemisia annua, including sesquiterpene lactones and flavonoid active substances, and effectively avoids the loss of active ingredients in traditional methods. In general, the present application provides an efficient, environmentally friendly and economical method for preparing a fat-soluble extract of Artemisia annua, which not only improves the utilization rate of Artemisia annua resources, but also provides a new source of natural active ingredients for the cosmetics industry, especially for toiletries.

[0004] In the first aspect, the present application provides a method for preparing a fat-soluble extract of Artemisia annua, which adopts the following technical scheme: A method for preparing a fat-soluble extract of Artemisia annua, comprising the following steps: Step 1: Take Artemisia annua leaves, dry them and grind them into residue to obtain Artemisia annua powder; Step 2: first, in a vacuum state, soak the Artemisia annua powder in a composite solvent mixed with ethanol, n-hexane, petroleum ether, succinic acid, and pentanediol, with a solid-liquid ratio of 1 g: (2-3) mL, then stir at 30-35° C. and 0.2-0.4 MPa for 20-30 min, and collect the extract; Step 3: Repeat step 2 2-3 times, combine the extracts, and remove the solvent by distillation under reduced pressure to obtain a preliminary extract of Artemisia annua; Step 4: Dewaxing the preliminary extract to obtain an Artemisia annua extract.

[0005] In the above technical scheme, the highly volatile aromatic oil in the Artemisia annua leaves has been volatilized during the drying process, and then the present application simultaneously extracts the Artemisia annua by using ethanol, n-hexane, and petroleum ether, and further adds succinic acid and pentanediol to effectively retain the sesquiterpene lactones and flavonoid active substances in the Artemisia annua, and effectively and comprehensively extracts the fat-soluble components in the Artemisia annua. In addition, the present application ensures that the fat-soluble components in the Artemisia annua are fully extracted by repeating the extraction steps for many times.

[0006] Preferably, the mass ratio of ethanol, n-hexane, petroleum ether, succinic acid and pentanediol is (5-10):(30-40):(20-30):(1-2):(0.5-1).

[0007] In the above technical scheme, the present application selects ethanol as a medium polar solvent, and its good solubility helps release the fat-soluble components in Artemisia annua; n-hexane and petroleum ether are used as low polar solvents to further extract the fat-soluble components in Artemisia annua, such as flavonoids; the further addition of succinic acid and pentanediol helps stabilize the active ingredients in the extraction process, and can enhance the solubility of the active ingredients and improve the extraction efficiency. Such a composite solvent system can not only fully extract the fat-soluble components in Artemisia annua, but also maintain the activity of these components, so that the extracted Artemisia annua fat-soluble extract has higher purity and better bioavailability.

[0008] Preferably, step 1 also includes a pretreatment step, and the operation of step 1 is as follows: take Artemisia annua leaves, spray the surface with a complex enzyme solution evenly after drying, enzymolyze for 16-24 hours under light-proof conditions at 38-40°C, then dry again, grind into a residue-like state, and obtain Artemisia annua powder.

[0009] In the above technical scheme, the present application further improves the release efficiency of active ingredients in Artemisia annua by enzymatic hydrolysis technology by adding a pretreatment step in step 1. The enzymes in the complex enzyme solution can specifically act on the cell wall of Artemisia annua, destroy its structure, and release the fat-soluble components that were originally tightly wrapped by the cell wall, providing a richer raw material basis for subsequent solvent extraction.

[0010] Preferably, the compound enzyme solution is prepared as follows: take pectinase and cellulase in a mass ratio of 1:(1-3), dissolve them in water, stir them evenly, and prepare a 0.2wt%-0.5wt% compound enzyme solution.

[0011] In the above technical scheme, the present application specifically selects pectinase and cellulase, which can work synergistically to effectively degrade the main components of the cell wall of Artemisia annua and promote the dissolution of fat-soluble components. At the same time, the enzymatic hydrolysis process is carried out under light-proof conditions to avoid the influence of light on enzyme activity and photosensitive components in Artemisia annua, thereby ensuring the enzymatic hydrolysis effect.

[0012] Preferably, the dewaxing treatment in step 4 is performed as follows: the preliminary extract is dissolved in ethanol, stirred evenly, and then subjected to ultrasonic-assisted extraction at 50-60° C. for 40 min at 200 W, the supernatant is taken, and the solvent is removed by reduced pressure distillation to obtain the Artemisia annua extract.

[0013] In the above technical solution, the present application promotes the separation of wax and fat-soluble components by ultrasonic-assisted dewaxing. At the same time, ethanol as a solvent can further dissolve and disperse the components in the preliminary extract, improve the dewaxing efficiency, and ensure the uniformity and thoroughness of the dewaxing process.

[0014] In the second aspect, the present application provides a fat-soluble extract of Artemisia annua using the following technical solution: A fat-soluble extract of Artemisia annua is prepared by the preparation method of the fat-soluble extract of Artemisia annua as described in the first aspect.

[0015] In the above technical scheme, the fat-soluble extract of Artemisia annua provided by the present application comprehensively covers the fat-soluble components in Artemisia annua, such as active substances such as artemisinin, sesquiterpene lactones and flavonoids, effectively avoiding the loss of some active ingredients in traditional methods, not only improving the utilization rate of Artemisia annua resources, but also ensuring the purity and activity of these components through a unique extraction process, further improving the use effect of the fat-soluble extract of Artemisia annua.

[0016] In a third aspect, the application of a fat-soluble extract of Artemisia annua provided by the present application adopts the following technical solution: The invention discloses an application of an Artemisia annua lipophilic extract in cosmetics. The Artemisia annua lipophilic extract is added into the cosmetics in an amount of 0.1wt% to 10wt%.

[0017] In the above technical scheme, this application applies the fat-soluble extract of Artemisia annua to the field of cosmetics, which not only can give the cosmetics a natural grass fragrance and a long-lasting fragrance, but also has good oil control, anti-inflammatory and antioxidant properties, significantly improving the nourishing and conditioning effects of cosmetics.

[0018] Preferably, the cosmetics include but are not limited to shampoo, conditioner, hair care essential oil, and hair care essence.

[0019] In the above technical scheme, by applying the Artemisia annua fat-soluble extract to shampoo, conditioner, hair care essential oil, and hair care essence, the scalp environment can be significantly improved, the scalp oil secretion can be regulated, and the generation of dandruff can be reduced. In particular, when the Artemisia annua fat-soluble extract is added to shampoo, the addition amount is only 0.2%-0.3%, which can effectively alleviate the problems of scalp sensitivity, redness and swelling, achieve the effect of relieving itching and removing dandruff, and provide all-round protection for scalp health. At the same time, the Artemisia annua fat-soluble extract can also keep the scalp fresh and non-greasy for a long time.

[0020] In summary, the present application includes at least one of the following beneficial technical effects: 1. The present application uses a composite solvent composed of ethanol, n-hexane, petroleum ether, succinic acid and pentanediol to extract Artemisia annua, thereby effectively and comprehensively extracting the fat-soluble components in Artemisia annua and ensuring the purity and activity of these components.

[0021] 2. This application adds a pretreatment step in step 1 and uses a composite enzyme solution to enzymatically hydrolyze the Artemisia annua leaves, which can significantly improve the preparation efficiency and quality of the Artemisia annua fat-soluble extract and provide a better source of natural active ingredients for the cosmetics industry.

[0022] 3. The fat-soluble extract of Artemisia annua of the present application has broad application prospects in the field of cosmetics. When added in an amount of 0.2wt%-0.3wt% in shampoo, it can effectively relieve itching and dandruff, promote scalp health, and make hair tougher and more shiny. DETAILED DESCRIPTION

[0023] In order to better illustrate the purpose, technical solutions and advantages of the present invention, the present invention will be further described below in conjunction with specific embodiments.

[0024] Example 1 A method for preparing a fat-soluble extract of Artemisia annua, comprising the following steps: Step 1: Take Artemisia annua leaves, dry them and crush them into residue to obtain Artemisia annua powder.

[0025] Step 2: Add 1 kg of Artemisia annua powder into the extraction tank, seal the tank, evacuate the air, then inject 2.5 kg of the composite solvent, stir at 32° C. and 0.3 MPa for 25 min, and collect the extract.

[0026] Step 3: Repeat step 2 twice, combine the extracts, perform reduced pressure distillation at an evaporation temperature of 45° C., remove the solvent, and obtain a preliminary extract of Artemisia annua.

[0027] Step 4: dissolve the preliminary extract in 65% ethanol with a solid-liquid ratio of 1 g:20 mL. After stirring evenly, perform ultrasonic-assisted extraction at 55°C for 40 min at 200 W. Then seal and let stand for 8 h. Take the supernatant and remove the solvent by vacuum distillation to obtain the Artemisia annua extract.

[0028] The composite solvent is prepared by mixing 65% ethanol, n-hexane, petroleum ether, succinic acid and pentanediol in a mass ratio of 8:35:25:1.5:0.8.

[0029] The fat-soluble extract of Artemisia annua L. of Example 1 was subjected to GCMS detection, and a total of 176 components were detected, and some of the identifiable components were as follows: Artemisinin type I 6.72%, deoxyartemisinin 17.47%, total flavonoids 15.63%, camphor 11.74%, Berkheyaradulene 11.355%, artemisinin 2.526%, aconene 20.685%, bicyclogalactene 1.563%, corydaline 0.404%, platambin-1,6-dione 2.79%, and the following Some trace elements: valerian terpene aldehyde, beta-neosyringaenol, leaf alcohol, phyton, isoborneol, lauryl ketone, myrtenol, myrtle aldehyde, artemisia ketone and its derivatives, spirulinatone, p-cymene, 1,8-cineole, wormwood alcohol, chrysanthemum ketone, chrysanthemum alcohol, guaiacene, isoacorus ketone, cedarwood, β-ostholene, δ-phellandrene, caryophyllene-9-ol, sabinol, β-caryophyllene, cubanne, caryophyllene oxide, caryophyllanol, amorpha-4,11-diene, (Z)-β-farnesene, menthenone, carveol, etc.

[0030] Example 2 A method for preparing a fat-soluble extract of Artemisia annua, which is different from that of Example 1, comprises the following steps: Step 1: Take Artemisia annua leaves, dry them and crush them into residue to obtain Artemisia annua powder.

[0031] Step 2: Add 1 kg of Artemisia annua powder into the extraction tank, seal the tank, evacuate the air, then inject 2 kg of the composite solvent, stir at 35° C. and 0.4 MPa for 30 min, and collect the extract.

[0032] Step 3: Repeat step 2 for 3 times, combine the extracts, perform reduced pressure distillation at an evaporation temperature of 45° C., remove the solvent, and obtain a preliminary extract of Artemisia annua.

[0033] Step 4: dissolve the preliminary extract in 65% ethanol with a solid-liquid ratio of 1 g:20 mL. After stirring evenly, perform 200 W ultrasonic-assisted extraction at 50°C for 40 min. Then seal and let stand for 8 h. Take the supernatant and remove the solvent by vacuum distillation to obtain the Artemisia annua extract.

[0034] The composite solvent is prepared by mixing 65% ethanol, n-hexane, petroleum ether, succinic acid and pentanediol in a mass ratio of 5:30:20:1:0.5.

[0035] Example 3 A method for preparing a fat-soluble extract of Artemisia annua, which is different from that of Example 1, comprises the following steps: Step 1: Take Artemisia annua leaves, dry them and crush them into residue to obtain Artemisia annua powder.

[0036] Step 2: Add 1 kg of Artemisia annua powder into the extraction tank, seal the tank, evacuate the air, then inject 3 kg of the composite solvent, stir at 30° C. and 0.2 MPa for 20 min, and collect the extract.

[0037] Step 3: Repeat step 2 twice, combine the extracts, perform reduced pressure distillation at an evaporation temperature of 45° C., remove the solvent, and obtain a preliminary extract of Artemisia annua.

[0038] Step 4: dissolve the preliminary extract in 65% ethanol with a solid-liquid ratio of 1 g:20 mL. After stirring evenly, perform ultrasonic-assisted extraction at 60°C for 40 min at 200 W. Then seal and let stand for 8 h. Take the supernatant and remove the solvent by vacuum distillation to obtain the Artemisia annua extract.

[0039] The composite solvent is prepared by mixing 65% ethanol, n-hexane, petroleum ether, succinic acid and pentanediol in a mass ratio of 10:40:30:2:1.

[0040] Example 4 A method for preparing a fat-soluble extract of Artemisia annua, which is different from that in Example 1, step 1 is as follows: Take Artemisia annua leaves, dry them, and evenly spray the surface with the complex enzyme solution at a dosage ratio of 1g:0.2mL. Perform enzymolysis at 40°C in the dark for 16h, then dry them again, grind them into residue, and obtain Artemisia annua powder.

[0041] The compound enzyme solution is prepared as follows: pectinase and cellulase in a mass ratio of 1:3 are dissolved in water and stirred evenly to prepare a 0.5wt% compound enzyme solution.

[0042] Example 5 A method for preparing a fat-soluble extract of Artemisia annua, which is different from that in Example 1, step 1 is as follows: Take Artemisia annua leaves, dry them, and evenly spray the complex enzyme solution on the surface with a dosage ratio of 1g:0.3mL. Perform enzymolysis at 38°C in the dark for 24h, then dry them again, grind them into residue, and obtain Artemisia annua powder.

[0043] The compound enzyme solution is prepared as follows: pectinase and cellulase in a mass ratio of 1:1 are dissolved in water and stirred evenly to prepare a 0.2wt% compound enzyme solution.

[0044] Comparative Example 1 A method for preparing a fat-soluble extract of Artemisia annua. The difference from Example 1 is that the composite solvent is prepared by mixing 65% ethanol, succinic acid and pentanediol in a mass ratio of 68:1.5:0.8.

[0045] Comparative Example 2 A method for preparing a fat-soluble extract of Artemisia annua. The method is different from Example 1 in that the composite solvent is prepared by mixing n-hexane, succinic acid and pentanediol in a mass ratio of 68:1.5:0.8.

[0046] Comparative Example 3 A method for preparing a fat-soluble extract of Artemisia annua. The method is different from Example 1 in that the composite solvent is prepared by mixing petroleum ether, succinic acid and pentanediol in a mass ratio of 68:1.5:0.8.

[0047] Comparative Example 4 A method for preparing a fat-soluble extract of Artemisia annua. The difference from Example 1 is that the composite solvent is prepared by mixing 65% ethanol, n-hexane and petroleum ether in a mass ratio of 8:35:25.

[0048] Comparative Example 5 A method for preparing a fat-soluble extract of Artemisia annua. The difference from Example 1 is that the composite solvent is prepared by mixing 65% ethanol, n-hexane, petroleum ether, glutaric acid and pentanediol in a mass ratio of 8:35:25:1.5:0.8.

[0049] Comparative Example 6 A method for preparing a fat-soluble extract of Artemisia annua. The difference from Example 1 is that the composite solvent is prepared by mixing 65% ethanol, n-hexane, petroleum ether, succinic acid and butanediol in a mass ratio of 8:35:25:1.5:0.8.

[0050] Comparative Example 7 A method for preparing a fat-soluble extract of Artemisia annua, which is different from Example 1 in that step 4 is not included.

[0051] The Artemisia annua extract that does not include step 4 was tested and it was found that the wax component content was too high, accounting for about 60% of the Artemisia annua extract. Such Artemisia annua extract is very limited in its application in cosmetics. Not only is the effect not obvious, but it is also easy to affect the system stability of the cosmetics. Therefore, it is necessary to dewax the Artemisia annua fat-soluble extract.

[0052] Verification of the efficacy of Artemisia annua fat-soluble extract in shampoo: Sample: the fat-soluble extract of Artemisia annua of Examples 1-5 and Comparative Examples 1-6.

[0053] 1. Test of oil control effect: Test principle: Human sebaceous gland cells (SZ95 cells) can be used as a cell model to study the oil control ability of cosmetics. The lipid content in human sebaceous gland cells after administration of the test substance, blank control and the test substance is determined by using Oil Red O (also known as Sudan Red 5B) dye to evaluate whether the test sample is effective in oil control.

[0054] Test method: Take samples, screen out the appropriate experimental concentration (cell survival rate relative to the blank control group ≥ 90%) by SZ95 cell activity experiment, and culture SZ95 cells in a culture medium (DMEM high-glucose culture medium) containing a certain sample for a period of time according to the results of the SZ95 cell activity assay. Use Oil Red O staining, pipette the treatment solution into a 96-well plate, put it into an ELISA reader to detect the absorbance at 490nm, and calculate the relative content of lipids in human sebaceous gland cells (%), the relative content of lipids in human sebaceous gland cells (%) = (A sample / A blank) * 100%. Among them, the blank control group is only added with the same mass of DMEM high-glucose culture medium.

[0055] The above test results are shown in Table 1.

[0056] 2. Test of anti-dandruff efficacy: Test principle: The anti-dandruff effect of anti-dandruff products is evaluated according to the standard of suspension quantitative method in "Evaluation Method for Antibacterial and Antibacterial Effect of Daily Chemical Products" QB / T2738-20237.3: Malassezia furfur is used as the indicator bacteria. After the prescribed time, the number of colonies on the test sample is recovered and the inhibition value is calculated. The anti-dandruff effect of the sample is evaluated by comparing the inhibition results of Malassezia between the control sample and the test sample.

[0057] Test method: Take a sample and prepare a solution of sample + Malassezia furfur ATCC44344 as the experimental group; take PBS (0.03 mol / L phosphate buffer) + Malassezia furfur ATCC44344 as the control group; take PBS as the blank control group.

[0058] Pour the above solution into the corresponding Malassezia furfur culture medium (Leeming and Notman culture medium), mix evenly, turn the plate over after it solidifies, and culture it in an incubator for 3 days. Count the live bacteria and calculate the Malassezia furfur inhibition rate (%). The Malassezia furfur inhibition rate (%) = (average colony number of the control group - average colony number of the experimental group) / average colony number of the control group * 100%.

[0059] The above test results are shown in Table 2.

[0060] 3. Test of soothing effect: Test principle: Referring to the Elson-Morgan hyaluronidase inhibition experiment, the soothing or anti-allergic ingredients in the sample react with hyaluronidase at 37°C in the presence of calcium chloride and potassium hyaluronate. The resulting reactant is then developed with the color developer P-DAB, and its absorbance is measured at 530nm by a UV-visible spectrophotometer, and then converted into a hyaluronidase inhibition rate by calculation.

[0061] Test method: Take the sample as the experimental group, take pure water as the negative control group, and take 1% dipotassium glycyrrhizinate solution as the positive control group.

[0062] Each group tested four groups of absorbance data, A, B, C, and D. The absorbance test method was as follows: calcium chloride, buffer solution, sample / negative control / positive control, sodium hydroxide, acetylacetone and other solutions were added to the test tube in sequence, mixed evenly, and then placed in boiling water, ice water, and room temperature for reaction, and then P-DAB color developer was added. After the solution inside reacted completely, the absorbance value was measured at a wavelength of 530nm.

[0063] The hyaluronidase inhibition rate (%) was calculated based on the absorbance value data of the four groups A, B, C, and D. Hyaluronidase inhibition rate (%) = [(CD)-(AB)] / (CD)*100%. Wherein, A is the absorbance of the reaction solution containing the sample and the enzyme; B is the absorbance of the reaction solution containing the sample and not containing the enzyme; C is the absorbance of the reaction solution containing the enzyme but not containing the sample; D is the absorbance of the reaction solution excluding the sample and the enzyme.

[0064] The above test results are shown in Table 3.

[0065] Table 1: Table 2: Table 3: Specifically combining Examples 1-5, Comparative Examples 1-6 and the test results, it can be seen that Examples 1-5 all perform well in lipid metabolism regulation, anti-dandruff and soothing effects.

[0066] Specifically combining the analysis of Example 1 and Comparative Examples 1-6, it can be seen that the difference between Example 1 and Comparative Examples 1-6 is that Comparative Examples 1-6 do not use a composite solvent mixed with ethanol, n-hexane, petroleum ether, succinic acid, and pentanediol, and Example 1 has better effects in lipid metabolism regulation, anti-dandruff and soothing effects. It can be seen that the synergistic effect of the composite solvent plays a key role in the quality of the Artemisia annua fat-soluble extract, and can effectively and more comprehensively extract the fat-soluble components in Artemisia annua, and ensure the purity and activity of these components, thereby showing better oil control, anti-dandruff and soothing effects, and can significantly improve the scalp environment, regulate the oil secretion of the scalp, and reduce the generation of dandruff.

[0067] This specific embodiment is merely an explanation of the present application and is not a limitation of the present application. After reading this specification, those skilled in the art may make modifications to the present embodiment without any creative contribution as needed. However, as long as it is within the scope of the claims of the present application, it shall be protected by the patent law.

Claims

1. A method for preparing a fat-soluble extract of Artemisia annua, characterized in that: The following steps are involved: Step 1: Take Artemisia annua leaves, dry them and grind them into residue to obtain Artemisia annua powder; Step 2: First, under vacuum, soak the Artemisia annua powder in a composite solvent mixed with ethanol, n-hexane, petroleum ether, succinic acid, and pentanediol, with a solid-liquid ratio of 1 g: (2-3) mL, then stir at 30-35° C. and 0.2-0.4 MPa for 20-30 minutes, and collect the extract; Step 3: Repeat step 2 2-3 times, combine the extracts, and remove the solvent by distillation under reduced pressure to obtain a preliminary extract of Artemisia annua; Step 4: Dewaxing the preliminary extract to obtain an Artemisia annua extract.

2. The method for preparing a fat-soluble extract of Artemisia annua according to claim 1, characterized in that: The mass ratio of the ethanol, n-hexane, petroleum ether, succinic acid and pentanediol is (5-10): (30-40): (20-30): (1-2): (0.5-1).

3. The method for preparing a fat-soluble extract of Artemisia annua according to claim 1, characterized in that: The step 1 also includes a pretreatment step, and the operation of the step 1 is as follows: take Artemisia annua leaves, spray the surface with a complex enzyme solution evenly after drying, enzymolyze for 16-24 hours under light-proof conditions at 38-40° C., then dry again, grind into a residue-like state, and obtain Artemisia annua powder.

4. The method for preparing a fat-soluble extract of Artemisia annua according to claim 3, characterized in that: The compound enzyme solution is prepared as follows: pectinase and cellulase in a mass ratio of 1:(1-3) are dissolved in water and stirred evenly to obtain a 0.2wt%-0.5wt% compound enzyme solution.

5. The method for preparing a fat-soluble extract of Artemisia annua according to claim 4, characterized in that: The dewaxing treatment operation of step 4 is as follows: dissolve the preliminary extract in ethanol, stir evenly, extract with 200W ultrasonic assisted extraction at 50-60°C for 40 minutes, take the supernatant, and remove the solvent by vacuum distillation to obtain the Artemisia annua extract.

6. A fat-soluble extract of Artemisia annua, characterized in that The extract is prepared by the method for preparing the artemisia annua fat-soluble extract according to any one of claims 1 to 5.

7. A use of the fat-soluble extract of Artemisia annua L. in cosmetics as claimed in claim 6, characterized in that: It is added to cosmetics in an amount of 0.1wt%-10wt%.

8. The use of a fat-soluble extract of Artemisia annua in cosmetics according to claim 7, characterized in that: The cosmetics include but are not limited to shampoo, conditioner, hair care essential oil, and hair care essence.

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