Preparation process and application of saffron crocus extract

Abstract

The invention relates to the field of natural product extraction, and provides a saffron crocus extract preparation process and application thereof, the saffron crocus extract is obtained by taking saffron crocus stigma as a raw material through ethanol extraction; wherein the saffron crocus extract contains crocin I and crocin II, and the mass percent of the crocin I and the crocin II in the saffron crocus extract is 2.8%-50%. The saffron crocus extract can absorb the wave band of 340 nm or above, and the transmittance of the sunscreen product containing the saffron crocus extract prepared by the saffron crocus extract at the wave band of 400 nm or above is reduced, so that the sunscreen product has excellent protection at the wave band of 400 nm or above, and has a synergistic effect on sunscreen and daily chemical products.

Description

Technical Field

[0001] The invention relates to the field of natural product extraction, and in particular to a saffron extract preparation process and application thereof. Background Art

[0002] Crocus sativus L. is a bulbous herb of the genus Crocus in the family Iridaceae. Its native habitat is from the Mediterranean coast to the foothills of the Himalayas, so it is called saffron, crocus or crocus in my country. Since ancient times, saffron has been widely used in the East and the West as a medicinal material, spice or dye. At present, the main producing areas of saffron are Greece, Spain and Iran. Only a small amount of saffron is cultivated in my country, mainly in Tibet, Yunnan, Zhejiang, Jiangsu, Shanghai and other places. Due to its extremely low yield (6kg·hm -2 It is listed as a rare and precious Chinese medicinal material in my country, which makes it expensive and is known as "plant gold".

[0003] In the prior art, patent CN113144057A discloses a saffron extract, a preparation method thereof, and an application thereof. In the saffron extraction process reported therein, the extraction rate of active substances is low and the extraction time is long, which is not conducive to industrial production. Patent CN117138388A discloses a preparation device and process for a saffron extract for aiding sleep. The relevant process equipment and process are reported. The extraction process is rough and there is no purification process, and the purity of the extract is low.

[0004] Therefore, there is an urgent need to develop a saffron extraction process with high extraction rate and simple extraction method in this field. Summary of the invention

[0005] The invention aims to provide a saffron extraction process with high extraction rate and simple extraction method, and specifically relates to a saffron extract preparation process and application thereof.

[0006] In a first aspect of the present invention, a saffron extract is provided, wherein the saffron extract is obtained by ethanol extraction using saffron stigma as raw material; wherein the saffron extract contains crocin I and crocin II, and the mass percentage of crocin I and crocin II in the saffron extract is: 2.8%-50%.

[0007] In another preferred embodiment, the mass percentage of crocin I and crocin II in the saffron extract is 2.8%-30%, preferably 2.8%-20%.

[0008] In another preferred embodiment, the mass ratio of crocin I to crocin II is: 1.5-3.5:0.5-1.5.

[0009] In another preferred embodiment, the saffron extract absorbs light in a wavelength band of ≥340 nm.

[0010] In another preferred embodiment, the saffron extract absorbs light in the wavelength range of 340-500nm.

[0011] In another preferred embodiment, the saffron extract absorbs light in the blue light band.

[0012] In another preferred embodiment, the saffron extract absorbs light in the wavelength range of 400-450nm.

[0013] In another preferred embodiment, the saffron extract is in solid or liquid state.

[0014] In the second aspect of the present invention, a composition is provided, comprising the saffron extract described in the first aspect and auxiliary materials.

[0015] In another preferred embodiment, the composition comprises a cosmetic additive rich in saffron extract.

[0016] In another preferred embodiment, in the cosmetic additive, the content of the saffron extract is 0.05-99wt%.

[0017] In another preferred embodiment, the auxiliary material is selected from the following group: maltodextrin, dextrin, silicon dioxide, 1,2-propylene glycol, 1,3-propylene glycol, 1,3-butylene glycol and glycerol.

[0018] In the third aspect of the present invention, there is provided a method for preparing the saffron extract as described in the first aspect, the method comprising the steps of:

[0019] (1) dispersing the raw material saffron stigma in ethanol, first performing flash extraction, then performing reflux extraction, and filtering with a sieve to obtain a first extract and a filter residue;

[0020] (2) extracting the filter residue obtained in step (1) in ethanol by refluxing to obtain a second extract;

[0021] (3) combining the first extract and the second extract, concentrating under reduced pressure to obtain a saffron extract;

[0022] (4) Purifying the saffron extract obtained in step (3) in an adsorption resin chromatography column to obtain the saffron extract.

[0023] In another preferred embodiment, the concentration of ethanol in step (1) is 50-75 v / v%, preferably 55-70 v / v%.

[0024] In another preferred embodiment, the mass volume ratio of saffron stigma to ethanol in step (1) is 1:8-20 kg / L.

[0025] In another preferred embodiment, the rotation speed of the flash extraction in step (1) is 1500-3000 rpm.

[0026] In another preferred embodiment, the extraction time of the flash extraction in step (1) is 1-3 minutes.

[0027] In another preferred embodiment, the extraction temperature of the reflux extraction in step (1) is 70-90°C.

[0028] In another preferred embodiment, the extraction time of the reflux extraction in step (1) is 1.0-3.0h.

[0029] In another preferred embodiment, the mass volume ratio of the filter residue to ethanol in step (2) is 1:8-20 (kg / L).

[0030] In another preferred embodiment, the temperature of the reduced pressure concentration in step (3) is 50°C-70°C.

[0031] In another preferred embodiment, the vacuum pressure of the reduced pressure concentration in step (3) is 0.060-0.08 MPa.

[0032] In another preferred embodiment, the reduced pressure concentration in step (3) is to reduce the pressure of the extract to 0.5 to 2.2 times the amount of the extract fed.

[0033] In another preferred embodiment, the purification in step (4) comprises the steps of:

[0034] Take saffron extract and load it on an adsorption resin chromatography column with a sample volume of V1. First elute it with a V2 volume of water, then analyze it with a V3 volume of ethanol, and collect the analyzed solution to obtain a purified saffron extract.

[0035] In another preferred embodiment, the sample loading volume V1 is 0.5-3BV.

[0036] In another preferred embodiment, the water washing volume V2 is 2-6BV.

[0037] In another preferred embodiment, the flow rate of the water wash is 2-6 BV / h.

[0038] In another preferred embodiment, the volume V3 of the ethanol analysis is 2-6BV.

[0039] In another preferred embodiment, the flow rate of ethanol is 2-6 BV / h.

[0040] In another preferred embodiment, the specification of the chromatography column is selected from the following group: HPD300, HPD700, X-5 adsorption resin, or a combination thereof.

[0041] In another preferred embodiment, the ratio of column height to bottom area diameter of the chromatography column is >3:1.

[0042] In the fourth aspect of the present invention, there is provided a use of the saffron extract according to the first aspect for preparing a product containing the saffron extract, wherein the product absorbs light in the wavelength band of ≥340 nm.

[0043] In another preferred embodiment, the product absorbs light in the wavelength range of 340-500nm.

[0044] In another preferred embodiment, the product absorbs light in the blue light band.

[0045] In another preferred embodiment, the product absorbs light in the wavelength range of 400-450nm.

[0046] In another preferred embodiment, the product comprises: (1) the saffron extract described in the first aspect; and (2) a sunscreen product.

[0047] In another preferred embodiment, the sunscreen product has a sunscreen effect of UVB and / or UVA.

[0048] In another preferred embodiment, the sunscreen product has sunscreen effects against UVB and UVA.

[0049] In another preferred example, the mass ratio of the sunscreen product to the saffron extract is ≤1:1.65.

[0050] In another preferred embodiment, the product has the functions of blue light protection and sun protection.

[0051] In another preferred embodiment, the product is a cosmetic.

[0052] In another preferred embodiment, the products include: facial care, body care, hair care, lip care, after-sun care, including cream, lotion, essence, facial mask, spray, gel, and paste.

[0053] In another preferred embodiment, the cosmetic further contains additional ingredients that can be used for makeup.

[0054] Compared with the prior art, the present invention has the following beneficial effects:

[0055] 1. The saffron extract of the present invention can absorb wavelengths above 340nm and has the effect of protecting against blue light.

[0056] 2. The saffron extract of the present invention has the effect of blue light protection in formula application. When 1% and 2% of the saffron extract are added to the sunscreen formula, the transmittance in the band above 400nm is significantly reduced, which has a synergistic effect on sunscreen and daily chemical products.

[0057] It should be understood that within the scope of the present invention, the above-mentioned technical features of the present invention and the technical features specifically described below (such as embodiments) can be combined with each other to form a new or preferred technical solution. Due to space limitations, they will not be described one by one here. BRIEF DESCRIPTION OF THE DRAWINGS

[0058] Figure 1 Shown is the UV spectrophotometer scanning spectrum of saffron stigma extract.

[0059] Figure 2 The HPLC spectrum of saffron stigma extract is shown.

[0060] Figure 3 Shown is the UV spectrophotometer scan spectrum of saffron petal extract.

[0061] Figure 4 An HPLC profile of saffron petal extract is shown.

[0062] Figure 5 UV spectrophotometer scanning spectra of different saffron extracts are shown.

[0063] Figure 6 Shown is the UV spectrophotometer scanning spectrum of the blended product of saffron stigma extract and commercially available organic sunscreen agent Tinosorb M.

[0064] Figure 7 The transmittance of the sunscreen product containing the saffron stigma extract of the present application is shown. DETAILED DESCRIPTION

[0065] The inventor has conducted extensive and in-depth research and, through a large number of experimental screenings, unexpectedly developed for the first time a process for extracting crocin I and crocin II from saffron quickly, efficiently and with low energy consumption. In the present invention, the saffron stigma is used as a raw material, and ethanol is first flash-extracted and then refluxed to obtain the saffron extract of the present invention, which can absorb the band above 340nm. The sunscreen product containing the saffron extract has a decreased transmittance in the band above 400nm, has excellent protection in the band above 400nm, and has a synergistic effect on sunscreen and daily chemical products. On this basis, the inventor has completed the present invention.

[0066] Terminology

[0067] In the present invention, the terms "saffron extract of the present invention" and "saffron stigma extract" both represent saffron extracts obtained by the preparation method of the present application and can be used interchangeably.

[0068] Crocin

[0069] Crocin, also known as crocin and crocin, is a series of ester glycosides formed by the combination of crocetin, a water-soluble carotene compound of saffron, and different sugars. It is one of the main active ingredients of saffron, including crocin-1 (crocin I), crocin-2 (crocin II), crocin-3, crocin-4 and crocin acid, which has central hypotension, prevention and treatment of atherosclerosis, anti-thrombosis, anti-platelet aggregation, anti-myocardial ischemia, anti-inflammatory and treatment of soft tissue damage, gallbladder protection, anti-tumor and other pharmacological effects, and is widely used in food, medicine, chemical industry and other industries, and is mostly used in the treatment of cardiovascular diseases and fatty liver patients. In the present application, the saffron extract (saffron extract) contains crocin I and crocin II, and the mass percentage of crocin I and crocin II in the saffron extract is: 2.8%-50%. The structures of crocin I and crocin II are shown below:

[0070] Crocin I

[0071]

[0072] Crocin II

[0073]

[0074] The present invention will be further described below in conjunction with specific examples. It should be understood that these examples are only used to illustrate the present invention and are not intended to limit the scope of the present invention. The experimental methods in the following examples without specifying specific conditions are usually based on conventional conditions or the conditions recommended by the manufacturer. Unless otherwise stated, percentages and parts are by weight.

[0075] Example 1 Extraction experiment of different parts of saffron

[0076] In this embodiment, different parts of saffron were extracted, and the spectrum was scanned by an ultraviolet spectrophotometer to explore the band absorption effect of the extracts from different parts of saffron.

[0077] 1.1 Saffron Stigma Extract

[0078] Accurately weigh 0.1000 g of saffron stigma and place it in a single-mouth bottle, add 50 ml of 60% ethanol, weigh the bottle, reflux extraction for 2 hours, add 60% to make up to the weight before reflux, take the supernatant and dilute it 10 times the volume to obtain the saffron stigma extract.

[0079] The saffron stigma extract was scanned by UV spectrophotometer and the maximum absorption peak was observed. Figure 1 As shown. Figure 1The absorption wavelength of peak No. 1 is 459.00nm, the absorption wavelength of peak No. 2 is 434.00nm, the absorption wavelength of peak No. 3 is 321.00nm, the absorption wavelength of peak No. 4 is 254.00nm, and the absorption wavelength of peak No. 5 is 206.00nm.

[0080] The saffron stigma extract was tested by high performance liquid chromatography. At 440nm, crocin I and crocin II were detected. Figure 2 shown.

[0081] 1.2 Saffron Petal Extract

[0082] Accurately weigh 0.1004 g of saffron petals and place them in a single-mouth bottle. Add 50 ml of 60% ethanol and weigh the bottle. After reflux extraction for 2 hours, add 60% to make up the weight to the weight before reflux. Take the supernatant and dilute it 10 times to obtain the saffron petal extract.

[0083] The saffron petal extract was scanned by UV spectrophotometer and the maximum absorption peak was observed. Figure 3 As shown. The absorption wavelength of peak 1 is 568.00nm, the absorption peak 2 is 520.00nm, the absorption peak 3 is 507.00nm, the absorption peak 4 is 459.00nm, the absorption peak 5 is 423.00nm, the absorption peak 6 is 351.00nm, the absorption peak 7 is 267.00nm, and the absorption peak 8 is 207.00nm.

[0084] The saffron petal extract was tested by high performance liquid chromatography, and no absorption was found at 440 nm. Figure 4 shown.

[0085] From the above results, it can be seen that saffron stigma extract has absorption bands at 206nm, 254nm, 321.00nm, 434nm and 459nm. At 440nm, there are the absorption peaks of crocin I and crocin II. The saffron stigma extract is enriched with the main active substances crocin I and crocin II. It can be predicted that the extract has good blue light protection effect.

[0086] Example 2: Extraction experiment of saffron stigma with different solvents

[0087] 2. Preparation of 160% ethanol-saffron stigma extract

[0088] Accurately weigh 0.1000 g of saffron stigma and put it into a single-mouth bottle, add 60% ethanol which is 20 times the amount of the raw material, reflux and extract at 80°C for 2.0 hours, and take the supernatant to obtain 60% ethanol-saffron stigma extract.

[0089] 2. Preparation of 275% ethanol-saffron stigma extract

[0090] Accurately weigh 0.1000 g of saffron stigma and put it into a single-mouth bottle, add 75% ethanol which is 20 times the amount of the raw material, reflux and extract at 80°C for 2.0 hours, and take the supernatant to obtain 60% ethanol-saffron stigma extract.

[0091] 2.3 Water Extraction - Preparation of Saffron Stigma Extract

[0092] Accurately weigh 0.1000 g of saffron stigma and put it into a single-mouth bottle, add 20 times the amount of water as the raw material, reflux extraction at 80°C for 2.0 hours, and take the supernatant to obtain the water-extracted saffron stigma extract.

[0093] 2.4 Results

[0094] The saffron stigma extract obtained above was diluted 25 times, and the absorbance was measured at 440 nm using an ultraviolet spectrophotometer. The results are shown in Table 1.

[0095] Table 1: Absorbance of saffron stigma extracts extracted with different solvents

[0096] Solvents Absorbance water 1.876 60% ethanol 2.571 75% ethanol 2.018

[0097] From the data in Table 1, it can be seen that the absorbance of saffron stigma extracted with 60% ethanol at 440nm is the strongest, followed by 75% ethanol. Therefore, 60% ethanol can be selected as the optimal extraction solvent.

[0098] Example 3 Preparation of Saffron Stigma Extract by Different Methods

[0099] 3.1 Preparation of saffron stigma extract E3-1 by reflux extraction

[0100] Accurately weigh 0.1000 g of saffron stigma and put it into a single-mouth bottle, add 20 times the amount of 60% ethanol as the raw material, reflux extraction at 80°C for 2.0 hours, and take the supernatant to obtain saffron stigma extract E3-1.

[0101] 3.2 Preparation of Saffron Stigma Extract E3-2 by Flash Extraction

[0102] Accurately weigh 0.1000 g of saffron stigma and place it in a single-mouth bottle, add 20 times the amount of raw material 60% ethanol, set the speed at 1500-3000 rpm, and flash extract for 2 minutes, take the supernatant to obtain saffron stigma extract E3-2.

[0103] 3.3 Preparation of Saffron Stigma Extract E3-3 by Flash + Reflux Extraction

[0104] Accurately weigh 0.1000 g of saffron stigma and place it in a single-mouth bottle, add 20 times the amount of raw material 60% ethanol, set the rotation speed to 1500-3000 rpm, and flash extract for 2 minutes, then reflux extraction at 80°C for 2.0 hours, and take the supernatant to obtain saffron stigma extract E3-3.

[0105] 3.4 Results

[0106] The saffron stigma extracts E3-1, E3-2 and E3-3 obtained above were diluted 100 times respectively, and the absorbance was measured at 440 nm using an ultraviolet spectrophotometer. The results are shown in Table 2.

[0107] Table 2: Absorbance of saffron stigma extract

[0108] sample Absorbance E3-1 0.684 E3-2 0.412 E3-3 0.807

[0109] From the data in Table 2, it can be seen that the method of saffron extract using flash + reflux extraction to prepare saffron stigma extract has the strongest absorbance at 440nm and a relatively high extraction rate. Flash + reflux extraction was selected as the extraction method.

[0110] Example 4 Preparation of Saffron Stigma Extract

[0111] In this embodiment, the saffron stigma is extracted, purified, and post-treated using the preferred conditions screened out above to obtain the saffron stigma extract of the present application.

[0112] Saffron stigma extract: weigh 100g of raw material saffron stigma, add 60% ethanol 8-20 times the amount of raw material, use a flash extractor to set the speed to 1500-3000rpm, the time is 2 minutes; then reflux extraction at 80°C for 2.0 hours; the obtained extract is naturally filtered through a 300-mesh screen to obtain a filtrate; take the remaining filter residue, add 60% ethanol 8-20 times the amount of raw material, and reflux extraction at 80°C for 2.0 hours. The combined filtrate is concentrated under reduced pressure (50°C-70°C, vacuum pressure 0.060-0.08Mpa) to 0.5-2.2 times the amount of feed, to obtain 100g of saffron stigma extract.

[0113] Purification: Take the saffron stigma extract obtained above, load it on 1-4 times the amount of the extract on a HPD300, HPD700 or X-5 adsorption resin chromatography column, the loading flow rate is 1-4BV / h, after the loading is completed, first elute with water, the water washing flow rate is 2-6BV / h, and the water elution volume is 2-6BV; then use a mass percentage concentration of 50-75% ethanol solution for analysis, the analysis flow rate is 2-6BV / h, the ethanol analysis volume is 2-6BV, collect the analysis solution, and concentrate it under reduced pressure (50℃-60℃, vacuum pressure 0.060~0.08Mpa) to 50%~100% of the feeding amount to obtain saffron stigma extract E4.

[0114] Post-processing: In daily chemicals, the saffron extract can be prepared into a powder or liquid extract. The purified extract can be dried into powder, or dried into powder together with maltodextrin and dextrin; or dispersed into liquid extract using solvents such as 1,2-propylene glycol, 1,3-propylene glycol, 1,3-butylene glycol and glycerol.

[0115] Example 5 Preparation of Saffron Stigma Extract

[0116] In this example, the preparation method is the same as that of Example 4, except that the ethanol extraction concentration is 75% ethanol, and saffron stigma extract E5 is prepared.

[0117] Example 6 Preparation of Saffron Stigma Extract

[0118] In this example, the preparation method is the same as that of Example 4, except that: reflux extraction is performed at 80° C. for 3.0 hours to prepare saffron stigma extract E6.

[0119] Example 7 Characterization of Saffron Stigma Extract

[0120] In this embodiment, the saffron stigma extract prepared by the method of the present application was taken, and the absorption spectrum of the extract was scanned by ultraviolet spectrophotometer, and tested by high performance liquid chromatography at 440nm, crocin I and crocin II.

[0121] The saffron stigma extract prepared by the method of the present application was diluted 100 times in volume, the spectrum was scanned with an ultraviolet spectrophotometer, and the maximum absorption peak was observed. The results are shown in Table 3.

[0122] Table 3: Absorbance of saffron stigma extract of the present application

[0123] sample Absorbance E4 0.815 E5 0.786 E6 0.798

[0124] The saffron stigma extract (lyophilized powder with 75% maltodextrin added) prepared by the method of the present application was tested by high performance liquid chromatography. At 440 nm, crocin I and crocin II were detected. The results are shown in Table 4.

[0125] Table 4: Components of the saffron stigma extract of the present application

[0126] sample Crocin I Crocin II E4 4.89％ 1.71％ E5 4.61％ 1.55％ E6 4.71％ 1.63％

[0127] Comparative Example Ultraviolet absorption test of the saffron stigma extract of the present application and the commercially available saffron extract

[0128] In this example, the saffron stigma extract prepared by the method of the present application and two commercially available saffron extracts were diluted with water to a concentration of 0.05% of each saffron extract, and the absorption curve at 200-600 nm was measured with an ELISA instrument. The results are as follows: Figure 5 As shown in Table 5. The commercially available saffrons are: commercially available saffron extract 1 (Youran Bio Sens'flower SD-SC); commercially available saffron extract 2 (Shanghai Jiayu JY1607)

[0129] Table 5: UV absorption test results

[0130] sample The strongest absorption peak position Absorption peak intensity Saffron Stigma Extract E4 442nm 2.6 Commercially available saffron extract 1 273nm 0.724 Commercially available saffron extract 2 264nm 0.249

[0131] From the above results, it can be seen that the saffron extract of the present application can absorb 340-500nm, with the maximum absorption peak at 442nm, while the two commercially available saffrons do not have the absorption in the band above 340nm.

[0132] Example 8 Compound test of saffron extract and organic sunscreen

[0133] In this example, the saffron stigma extract prepared by the method of the present application and a commercially available organic sunscreen such as Tinosorb M (BASF) were diluted with water, and the absorption curve at 200-600 nm was measured by an ELISA instrument. The spectrum of the obtained composite product was scanned by an ultraviolet spectrophotometer, and the maximum absorption peak was observed. The results are as follows: Figure 6 shown.

[0134] From the results in the figure, it can be seen that the compound product of organic sunscreen with the addition of saffron stigma extract can achieve protection against UVB+UVA+blue light, especially the compound product with a mass ratio of Tinsorb M to the saffron stigma extract of the present application being less than 1:1.65, while achieving an effect equivalent to that of commercially available sunscreens, can also provide protection above 400nm, indicating that the compound of the saffron stigma extract of the present application and the organic sunscreen can achieve full-band protection.

[0135] Example 9 Sunscreen product containing the saffron stigma extract of the present application

[0136] Preparation method of oil-in-water sunscreen cream

[0137] ① A sunscreen formula without the sunscreen enhancer of the present invention: a sunscreen formula system based on chemical sunscreens such as butyl methoxydibenzoylmethane, octocrylene, ethylhexyl salicylate, homosalate, bis-ethylhexyloxyphenol methoxyphenyl triazine, etc., to prepare a sunscreen lotion with a sunscreen effect and uniform appearance as a basic sunscreen.

[0138] ② A sunscreen formula with the sunscreen enhancer of the present invention added: select the basic sunscreen cream system in ①, and replace a small amount of water in the formula with the saffron stigma extract of the present invention in equal amounts at ratios of 0.5%, 1% and 2% respectively to prepare a sunscreen lotion.

[0139] UV2000S test:

[0140] ①Testing instruments

[0141] Labsphere Inc., USA, sun protection factor tester UV 2000S and Mettler analytical balance ME204 (accuracy 0.0001g, range 220g)

[0142] ②Test environment

[0143] Environmental requirements: Temperature: 25.0℃±2.0℃; Humidity: 50%±10%.

[0144] ③Testing process

[0145] First, evenly apply 0.0325g of glycerol on the PMMA plate and scan the blank template; use a syringe to drop the sample on the plate, the sample amount is 0.0325g, and try to make the sample evenly distributed on the plate; the plate used is a 5×5cm UV test PMMA plate, the frosted surface is the sample coating surface, and fingers should be avoided from direct contact with the front and back sides when using; use the fingertips to apply with a force of about 200g, weigh and record the sample amount; keep it away from light and let it stand for 20 minutes to form a film before testing, and use UV2000S to obtain the test results; repeat the above steps, test three plates for each sample and record the data. The results are as follows Figure 7 As shown in the figure, the data show that with the increase of saffron stigma extract and the increase of added amount, the protection effect in the 400-450nm band is significantly improved, which is manifested as a decrease in transmittance and has a metered dependence.

[0146] discuss

[0147] In the prior art, patent CN109303748 natural plant sunscreen composition and its preparation method and application in sunscreen cosmetics, sunscreen composition contains saffron extract, but the product has a protective effect on ultraviolet rays, ultraviolet rays are under the 400nm wavelength band, and its extraction product has no protective effect on blue light. In the present application, saffron stigma is used as raw material, ethanol is first flash extracted and then reflux extracted, and the saffron extract of the present invention can absorb the band above 340nm, thereby improving the protection of the blue light band (blue light transmittance decreases). In addition, in the present invention, after the saffron extract of the present application is added to the sunscreen formula, the transmittance of the band above 400nm is also significantly decreased after detection, indicating that the saffron extract obtained by the present invention has good absorption for the blue light band, has a synergistic effect on sunscreen and daily chemical products, and provides new ideas for the application of daily chemical products.

[0148] All documents mentioned in the present invention are cited as references in this application, just as each document is cited as reference individually. In addition, it should be understood that after reading the above teachings of the present invention, those skilled in the art can make various changes or modifications to the present invention, and these equivalent forms also fall within the scope defined by the claims attached to this application.

Claims

1. A saffron extract, characterized in that The saffron extract is obtained by ethanol extraction using saffron stigma as raw material; wherein the saffron extract contains crocin I and crocin II, and the extract is prepared by the following steps: (1) dispersing the raw material saffron stigma in ethanol, first performing flash extraction, then performing reflux extraction, and filtering with a sieve to obtain a first extract and a filter residue; (2) extracting the filter residue obtained in step (1) in ethanol by refluxing to obtain a second extract; (3) combining the first extract and the second extract, concentrating under reduced pressure to obtain a saffron extract; (4) Purifying the saffron extract obtained in step (3) in an adsorption resin chromatography column to obtain the saffron extract.

2. The saffron extract according to claim 1, characterized in that The saffron extract absorbs light in a wavelength band of ≥340nm.

3. A composition, characterized in that The composition comprises the saffron extract according to claim 1 and auxiliary materials.

4. The composition according to claim 3, characterized in that The auxiliary material is selected from the following group: maltodextrin, dextrin, silicon dioxide, 1,2-propylene glycol, 1,3-propylene glycol, 1,3-butylene glycol and glycerol.

5. A method for preparing the saffron extract according to claim 1, characterized in that: The method comprises the steps of: (1) dispersing the raw material saffron stigma in ethanol, first performing flash extraction, then performing reflux extraction, and filtering with a sieve to obtain a first extract and a filter residue; (2) extracting the filter residue obtained in step (1) in ethanol by refluxing to obtain a second extract; (3) combining the first extract and the second extract, concentrating under reduced pressure to obtain a saffron extract; (4) Purifying the saffron extract obtained in step (3) in an adsorption resin chromatography column to obtain the saffron extract.

6. The method according to claim 5, characterized in that The concentration of ethanol in step (1) is 50-75 v / v%.

7. The method according to claim 5, characterized in that The mass volume ratio of saffron stigma to ethanol in step (1) is 1:8-20kg / L.

8. The method according to claim 5, characterized in that The extraction time of the flash extraction in step (1) is 1-3 minutes.

9. The method according to claim 5, characterized in that The extraction time of the reflux extraction in step (1) is 1.0-3.0h.

10. A use of the saffron extract according to claim 1, characterized in that: Used for preparing a product containing saffron extract, wherein the product absorbs light in the wavelength band of ≥340nm.

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