Preparation method of cold-soaked herba epimedii
Through the preparation method of cold-soaked epimedium, the stratum corneum of epimedium leaves is destroyed by soaking yeast aqueous solution. Combined with appropriate temperature and time treatment, traditional epimedium supplements are not suitable for modern life and have stable medicinal efficacy. The high leaching rate and drug effect maintenance effect has been achieved.
Patent Information
- Application Number
- CN202411365121.6
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2024-09-29
- Publication Date
- 2024-12-27
AI Technical Summary
The existing epimedium supplements are not suitable for modern fast-paced life, and traditional brewing methods affect the efficacy and taste, and the drying time and temperature of dry leaves affect the efficacy. Fresh leaves are prone to mold and have a low leachate rate, which affects the drinking effect. .
The preparation method of cold-beamed epimedium is adopted, including fermentation, spreading, finishing, cooling, rolling and drying steps, soaking freshly destemmed epimedium leaves with high-sugar yeast aqueous solution, destroying the cuticle and changing the leaf structure. Improve the dissolution rate of effective substances and ensure the maintenance of drug efficacy by controlling temperature and time.
The high leaching rate epimedium supplements that adapt to modern fast-paced life have been achieved, ensuring the stability of the medicinal effect and improving the taste, and avoiding the volatility of active ingredients and the reduction of medicinal efficacy caused by long-term placement in traditional methods.
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Abstract
Description
Technical Field
[0001] The invention relates to a preparation method of cold-brewed epimedium. Background Art
[0002] Epimedium, also known as Xianlingpi and Gangqian, is listed as a medium-grade product in Shennong's Herbal Classic. It has the effects of tonifying kidney yang, strengthening tendons and bones, and removing rheumatism. It is used to treat impotence and spermatorrhea, tendon and bone weakness, rheumatic pain, numbness and cramps caused by kidney yang deficiency. It is a clinical Chinese medicine. Zhang County, Dingxi City, Gansu Province is cold and damp, which is suitable for the growth of epimedium, and epimedium is in short supply.
[0003] At present, our hospital, Gansu University of Chinese Medicine and Gansu Agricultural University have established a wild epimedium domestication, breeding and product research and development base in Zhang County. Currently, the epimedium sold locally is mainly fresh leaves or dried leaves. Fresh leaves are prone to mold and hair growth if not stored properly, while dried leaves are affected by factors such as drying time and temperature, which will also affect the efficacy of the medicine.
[0004] Epimedium supplements were born out of application. Regular consumption has a good health-care effect and stable quality. However, traditional epimedium needs to be boiled or brewed in boiling water, which is not suitable for the fast-paced life of today. For example, if you boil it in boiling water in the morning, you have to go to work before it cools down and you cannot drink the brewed epimedium. You can only drink it after get off work in the afternoon. As we all know, the active ingredients of epimedium are relatively complex. Long-term storage may cause reactions among various substances and volatilization of aromatic substances, thus affecting the effect and taste of epimedium supplements.
[0005] Currently, there is no epimedium supplement that can adapt to the fast-paced modern life, and the product requires a high extraction rate without affecting the drinking effect. Summary of the invention
[0006] The invention provides a preparation method of cold-brewed epimedium, and solves the technical problem of designing an epimedium tonic adapting to the modern fast-paced life, and the product requires a high leaching rate without affecting the drinking effect.
[0007] In order to solve the above technical problems, the present invention adopts the following technical solutions: A method for preparing cold-brewed epimedium, characterized in that the steps are as follows: 1) Fermentation; the fermentation is performed by soaking fresh de-stemmed Epimedium leaves in an aqueous solution containing high-sugar yeast for 20 to 60 minutes at a temperature of 20 to 30°C; 2) spreading; taking out the freshly de-stemmed epimedium leaves soaked in step 1) and spreading them to obtain spread epimedium leaves; 3) Fixing the green leaves; Fixing the green leaves of the spread epimedium to obtain the fixed green epimedium leaves; 4) Cooling: Cooling the withered Epimedium leaves; 5) rolling and twisting; rolling and twisting the cooled epimedium leaves into strips, and rolling the epimedium leaves; 6) Drying: The rolled epimedium leaves are dried at a temperature of 70-85°C to obtain cold-brewed epimedium.
[0008] The mass ratio of the high-sugar yeast to the aqueous solution containing high-sugar yeast is 0.2-1:99-99.8; the mass ratio of the fresh de-stemmed epimedium leaves to the aqueous solution containing high-sugar yeast is 1:12-15.
[0009] The spreading thickness is 2 to 4 cm, the spreading temperature is 15 to 30° C., and the spreading time is 1.5 to 4 hours; The spreading thickness refers to the thickness of the spread epimedium leaves.
[0010] The fixing temperature is 160-200° C. and the time is 2-4 minutes.
[0011] The cooling time is 6 to 10 minutes.
[0012] The drying step is to dry the cold-brewed epimedium until the water content of the cold-brewed epimedium is less than 7%. Other beverages may also be added to the cold-brewed epimedium; The other beverages include one or more of green tea, black tea and milk shake.
[0013] The invention has the following beneficial technical effects: 1) The present application ferments fresh de-stemmed epimedium leaves to destroy the cuticle on the surface of the epimedium leaves, thereby changing the structure of the leaves and facilitating the dissolution of effective substances.
[0014] 2) The yeast selected in this application is a high-sugar yeast, which can destroy the surface of epimedium leaves in a short time without changing the substances in the epimedium leaves. This is because fresh epimedium leaves do not lose their internal activity after being picked. Long-term soaking in water puts the leaves in an oxygen-deficient state, changing their environment in the air, which may change the activity of enzymes in the fresh leaves, thereby affecting the content of substances in the leaves, thereby affecting the extraction rate of effective substances. DETAILED DESCRIPTION
[0015] In order to better understand the above technical solution, the technical solution of the present invention is described in detail below in conjunction with preferred examples. Example 1
[0016] A method for preparing cold-brewed epimedium is carried out according to the following steps: 1) Fermentation: Soak fresh de-stemmed Epimedium leaves in an aqueous solution containing high-sugar yeast for 40 minutes at a temperature of 25°C; 2) spreading; taking out the freshly de-stemmed epimedium leaves soaked in step 1), spreading them, the spreading thickness is 2 to 3 cm, the natural spreading temperature is 15 to 20° C., and the spreading time is 2.5 h, to obtain spread epimedium leaves; 3) Fixing the green leaves; Fixing the green leaves of the spread epimedium to obtain the fixed green epimedium leaves; 4) Cooling: Cooling the withered Epimedium leaves; 5) rolling and twisting; rolling and twisting the cooled epimedium leaves into strips, and rolling the epimedium leaves; 6) Drying The rolled epimedium leaves are dried at a temperature of 75° C. until the moisture content of the cold-brewed epimedium is 5.5%, thereby obtaining the cold-brewed epimedium.
[0017] The mass ratio of high-sugar yeast to the aqueous solution containing high-sugar yeast is 0.5:99.5; the mass ratio of fresh de-stemmed epimedium leaves to the aqueous solution containing high-sugar yeast is 1:12.
[0018] The spreading thickness is the thickness of the spread epimedium leaves.
[0019] The microwave was used for killing the green tea, the temperature was 180℃, and the time was 3min.
[0020] The cooling time is 8 minutes. Example 2
[0021] A method for preparing cold-brewed epimedium is carried out according to the following steps: 1) Fermentation; the fermentation is performed by soaking fresh de-stemmed Epimedium leaves in an aqueous solution containing high-sugar yeast for 30 minutes at a temperature of 28°C; 2) spreading; taking out the freshly de-stemmed epimedium leaves soaked in step 1) and spreading them to obtain spread epimedium leaves; 3) Fixing the green leaves; Fixing the green leaves of the spread epimedium to obtain the fixed green epimedium leaves; 4) Cooling: Cooling the withered Epimedium leaves; 5) rolling and twisting; rolling and twisting the cooled epimedium leaves into strips, and rolling the epimedium leaves; 6) Drying The rolled epimedium leaves are dried at a temperature of 80° C. to obtain cold-brewed epimedium.
[0022] The mass ratio of high-sugar yeast to the aqueous solution containing high-sugar yeast is 0.5:99.5; the mass ratio of fresh de-stemmed epimedium leaves to the aqueous solution containing high-sugar yeast is 1:14.
[0023] The spreading thickness is 3-4 cm, the spreading temperature is 18-22°C, and the spreading time is 3 hours.
[0024] The spreading thickness refers to the thickness of the spread epimedium leaves.
[0025] Microwave sterilization was used with a temperature of 170°C and a time of 4 min.
[0026] The cooling time is 9 minutes.
[0027] The drying process is to dry the cold soaked epimedium until its moisture content is 6%. Example 3
[0028] A method for preparing cold-brewed epimedium, characterized in that the steps are as follows: 1) Fermentation; the fermentation is performed by soaking fresh de-stemmed Epimedium leaves in an aqueous solution containing high-sugar yeast for 50 minutes at a temperature of 22°C; 2) spreading; taking out the freshly de-stemmed epimedium leaves soaked in step 1) and spreading them to obtain spread epimedium leaves; 3) Fixing the green leaves; Fixing the green leaves of the spread epimedium to obtain the fixed green epimedium leaves; 4) Cooling: Cooling the withered Epimedium leaves; 5) rolling and twisting; rolling and twisting the cooled epimedium leaves into strips, and rolling the epimedium leaves; 6) Drying The rolled epimedium leaves are dried at a temperature of 75°C to obtain cold-brewed epimedium.
[0029] The mass ratio of high-sugar yeast to the aqueous solution containing high-sugar yeast is 0.6:99.4; the mass ratio of fresh de-stemmed epimedium leaves to the aqueous solution containing high-sugar yeast is 1:13.
[0030] The spreading thickness is 2-3 cm, the spreading temperature is 18-22° C., and the spreading time is 3 hours.
[0031] The spreading thickness refers to the thickness of the spread epimedium leaves.
[0032] The killing is carried out by microwave at a temperature of 200° C. and a time of 2 minutes.
[0033] The cooling time is 8 minutes.
[0034] The drying step is drying until the moisture content of the cold-soaked epimedium is 6%. Example 4
[0035] A method for preparing cold-brewed epimedium is carried out according to the following steps: 1) Fermentation; the fermentation is performed by soaking fresh de-stemmed Epimedium leaves in an aqueous solution containing high-sugar yeast for 25 minutes at a temperature of 28°C; 2) spreading; taking out the freshly de-stemmed epimedium leaves soaked in step 1) and spreading them to obtain spread epimedium leaves; 3) Fixing the green leaves; Fixing the green leaves of the spread epimedium to obtain the fixed green epimedium leaves; 4) Cooling: Cooling the withered Epimedium leaves; 5) rolling and twisting; rolling and twisting the cooled epimedium leaves into strips, and rolling the epimedium leaves; 6) Drying: Drying the rolled epimedium leaves at a temperature of 80° C. to obtain cold-brewed epimedium; 7) Mix cold brewed epimedium and green tea to make cold brewed epimedium tea.
[0036] The mass ratio of high-sugar yeast to the aqueous solution containing high-sugar yeast is 0.8:99.2; the mass ratio of fresh de-stemmed epimedium leaves to the aqueous solution containing high-sugar yeast is 1:14.
[0037] The spreading thickness is 2.5-3 cm, the spreading temperature is 20-23° C., and the spreading time is 2.5 h.
[0038] The spreading thickness refers to the thickness of the spread epimedium leaves.
[0039] The killing is performed by drum killing at a temperature of 180° C. for 3 minutes.
[0040] The cooling time is 8 minutes.
[0041] The drying step is drying until the moisture content of the cold-soaked epimedium is 6%.
[0042] The beneficial effects of the present invention are further illustrated below in conjunction with test data: 1.1 Experimental location: Gansu Smart Agriculture Engineering Technology Research Center.
[0043] 1.2 Experimental test: total flavonoids (mg / g); total flavonoids include epimedin A (mg / g), epimedin B (mg / g), epimedin C (mg / g) and icariin (mg / g).
[0044] 1.3 Test samples: Fresh epimedium leaves were collected from the epimedium base in Zhang County.
[0045] Comparison 1 (except that fermentation was not performed, all other aspects were consistent with Example 1), Comparison 2 (except that step 2 was performed first and then step 1), all other aspects were consistent with Example 1), Comparison 3 (except that the yeast used was low-sugar yeast, all other aspects were consistent with Example 1), Comparison 4 (except that the soaking time in step 1 was 2 hours, all other aspects were consistent with Example 1), and cold-soaked epimedium prepared in Examples 1 to 3.
[0046] 1.4 Experimental method: Take 0.2g of each sample, retain 4 decimal places, place them in stoppered conical flasks, accurately add 400 times the amount of water at 25°C and 98°C, soak and extract for 1h, filter, take a quarter of the filtrate, place it in an evaporating dish, evaporate to dryness in a water bath, dissolve it with dilute ethanol, make up to the volume in a 5mL volumetric flask, filter with a 0.45μm filter membrane to obtain samples Comparison 1 to Comparison 4 and Example 1 to Example 3.
[0047] 1.5 Detection method: Determine according to the method of the 2020 edition of the Pharmacopoeia of the People's Republic of China.
[0048] Except for the experimental treatment, other operations were the same in this experiment.
[0049] 2 Results and analysis The test results of epimedin A (mg / g), epimedin B (mg / g), epimedin C (mg / g), icariin (mg / g) and total flavonoids (mg / g) are shown in Table 1 Table 1
[0050] It can be seen from the data in Table 1 that the total flavonoids in the unfermented comparative 1 are very different when soaked in water at 25°C and 98°C, that is, if cold soaking is used, it will cause a waste of effective ingredients.
[0051] It can be seen from the data of Comparison 2 and Example 1 in Table 1 that the order of fermentation changes and the results of the soaking also change. The total flavonoids soaked in Comparison 2 are significantly lower than those in Example 1. This may be because the epimedium leaves become soft due to spreading and the leaf structure changes, causing some effective substances to be leached during fermentation, resulting in the total flavonoids being significantly lower than the total flavonoids in Example 1.
[0052] It can be seen from the data of Comparison 3 and Example 1 in Table 1 that the yeast used in the fermentation also affects the soaking results. The total flavonoids soaked in Comparison 3 are significantly lower than those in Example 1, and the difference in total flavonoids in Comparison 3 itself using water soaking at 25°C and 98°C is very large, that is, Comparison 3 has incomplete soaking of effective substances, and using cold soaking will further waste effective ingredients in the morning.
[0053] It can be seen from the data of Comparison 4 and Example 1 in Table 1 that the soaking time also affects the results. The total flavonoids amount soaked in Comparison 4 is significantly lower than that in Example 1. This may be due to the long soaking time causing the effective substances to flow out, or it may be due to the soaking in anoxic state causing changes in the enzymes in the leaves, leading to the decomposition of the effective substances.
Claims
1. A method for preparing cold-brewed epimedium, characterized in that: Follow the steps below to proceed 1) Fermentation; the fermentation is performed by soaking fresh de-stemmed Epimedium leaves in an aqueous solution containing high-sugar yeast for 20 to 60 minutes at a temperature of 20 to 30°C; 2) spreading; taking out the freshly de-stemmed epimedium leaves soaked in step 1) and spreading them to obtain spread epimedium leaves; 3) Fixing the green leaves; Fixing the green leaves of the spread epimedium to obtain the fixed green epimedium leaves; 4) Cooling: Cooling the withered Epimedium leaves; 5) rolling and twisting; rolling and twisting the cooled epimedium leaves into strips, and rolling the epimedium leaves; 6) Drying: The rolled epimedium leaves are dried at a temperature of 70-85°C to obtain cold-brewed epimedium.
2. The method for preparing cold-brewed Epimedium as claimed in claim 1, characterized in that: The mass ratio of the high-sugar yeast to the aqueous solution containing high-sugar yeast is 0.2-1:99-99.8; the mass ratio of the fresh de-stemmed epimedium leaves to the aqueous solution containing high-sugar yeast is 1:12-15.
3. The method for preparing cold-brewed Epimedium as claimed in claim 1, characterized in that: The spreading thickness is 2 to 4 cm, the spreading temperature is 15 to 30° C., and the spreading time is 1.5 to 4 hours; The spreading thickness refers to the thickness of the spread epimedium leaves.
4. The method for preparing cold-brewed Epimedium as claimed in claim 1, characterized in that: The fixing temperature is 160-200° C. and the time is 2-4 minutes.
5. The method for preparing cold-brewed Epimedium as claimed in claim 1, characterized in that: The cooling time is 6 to 10 minutes.
6. The method for preparing cold-brewed Epimedium as claimed in claim 1, characterized in that: The drying step is drying until the water content of the cold-soaked epimedium is less than 7%.
7. The method for preparing cold-brewed Epimedium as claimed in claim 1, characterized in that: Other drinks can also be added to the cold-brewed epimedium; The other beverages include one or more of green tea, black tea and milk shake.