Application of Shutchmanspipe bungeana leaf extract

Abstract

The invention relates to a natural extract, in particular to an application of a spunlace spunlace leaf extract in preparation of a medicine for treating erectile dysfunction, and the spunlace spunlace leaf extract is a petroleum ether phase extract. The obtained petroleum ether phase extract can increase the number of times of back climbing and ejaculation of rats, shorten the incubation period of back climbing and ejaculation, induce and maintain penile erection, increase the content of nitric oxide, testosterone, luteinizing hormone and follicle-stimulating hormone, improve sperm motility and sperm motility rate, improve sperm quality and reduce the content of PDE5 and ADA in penis tissue, and can be used for preparing a medicine for treating the rats. The traditional Chinese medicine composition also has the effects of improving sex hormone secretion disorder of rats and stabilizing the hormone level, and has a remarkable effect on treating erectile dysfunction.

Description

Technical Field

[0001] The invention relates to a natural extract, and in particular to an application of a leaf extract of the Chinese hydrangea in preparing a medicine for treating erectile dysfunction. Background Art

[0002] Erectile dysfunction (ED) is a common clinical disease, which is manifested by decreased libido, inability of the penis to erect normally within a certain period of time, or to last for a sufficient period of time. It belongs to the category of "impotence" in traditional Chinese medicine. It is often seen in the clinical case of kidney yang deficiency and is one of the important diseases that plague men around the world. With the changes in work and lifestyle, the incidence of ED has a trend of getting younger, and its prevalence rate in young men is as high as 30%. In addition, with the increasing number of anxious people, patients who take the antidepressant anxiety drug paroxetine for a long time may experience sexual dysfunction, such as orgasm disorder, difficulty in male penis erection, delayed male ejaculation, delayed orgasm, and impotence, which seriously affect the quality of life of patients and increase non-compliance with treatment, all of which may aggravate secondary depression and anxiety.

[0003] Currently, phosphodiesterase 5 inhibitors (PDE5i) are the first-line drugs for the treatment of ED. Currently, there are multiple PDE5 inhibitors, including sildenafil, tadalafil, vardenafil and avanafil, which are marketed for the treatment of ED, and their half-lives vary. However, existing PDE5 inhibitors have side effects such as heartburn, headache, facial flushing, and allergic reactions. Long-term use can cause liver and kidney damage. In addition, there are problems such as low specificity, poor water solubility, short half-life, and some patients do not respond to the drug. Therefore, it is of great significance and application value to develop drugs with small side effects and good responsiveness for the treatment of erectile dysfunction.

[0004] Wendlandia uvariifolia is a tree plant of the Rubiaceae family. Its Latin name is Wendlandia uvariifolia subsp. Chinensis (Merr.) Cowan. It is used by Li medicine in Qiongzhong County, Hainan Province to treat herpes zoster. The treatment methods include oral decoction and external application of fresh leaves. As a natural product, Wendlandia uvariifolia has the advantages of wide sources, safety, and low toxicity and side effects. However, its efficacy and pharmacology are still unclear, and there are no reports on its use in ED. Summary of the invention

[0005] The invention aims to provide a leaf extract of the Chinese water hyacinth tree, and to use the extract as a raw material to prepare a medicine which has a wide source of raw materials, a safe preparation process, no toxic side effects on the human body, good reactivity and significant therapeutic effect.

[0006] The above-mentioned purpose of the present invention is achieved through the following technical solutions:

[0007] The invention discloses an application of a leaf extract of the Chinese water hyacinth tree in preparing a drug for alleviating and / or treating erectile dysfunction. The leaf extract of the Chinese water hyacinth tree is a petroleum ether phase extract of the leaves of the Chinese water hyacinth tree.

[0008] The present invention also discloses a method for preparing a leaf extract of the Chinese water hyacinth tree. The leaf extract of the Chinese water hyacinth tree is prepared by the following method:

[0009] (1) drying the leaves of the Chinese water hyacinth tree and then crushing them, soaking them in 4 to 8 times the amount of ethanol at room temperature, and then filtering to obtain a filtrate, repeating this operation at least three times, collecting and combining the filtrate, and concentrating the filtrate by reducing pressure using a rotary evaporator, and freeze-drying the ethanol extract of the leaves of the Chinese water hyacinth tree;

[0010] (2) The ethanol extract of the leaves of the Chinese water hyacinth tree is dissolved in water, and extracted with a petroleum ether solution for multiple times until the extract phase is colorless, the petroleum ether extracts are combined, the pressure is reduced and concentrated using a rotary evaporator, and the freeze-dried product is the petroleum ether phase extract of the leaves of the Chinese water hyacinth tree.

[0011] In an optional embodiment, the medicine or health food having the effect of alleviating and / or treating erectile dysfunction is prepared using the petroleum ether extract of the leaves of the Chinese water hyacinth as an active ingredient.

[0012] In an optional embodiment, the dosage form of the medicine or health food is capsule, pill, tablet or granule.

[0013] Compared with the prior art, the present invention has the following beneficial effects:

[0014] The Chinese water hyacinth leaf extract of the present invention is obtained by ethanol extraction and then extraction with petroleum ether. The obtained petroleum ether phase extract can increase the number of rats' back climbing and ejaculation, shorten the back climbing and ejaculation latency, induce and maintain penile erection, and can also increase the content of nitric oxide, testosterone, luteinizing hormone and follicle stimulating hormone, improve sperm motility and sperm viability, improve sperm quality, reduce the content of PDE5 and ADA in penile tissue, and also has the effect of improving the disorder of sex hormone secretion in rats and stabilizing hormone levels, and has a significant effect on the treatment of erectile dysfunction. BRIEF DESCRIPTION OF THE DRAWINGS

[0015] Figure 1 is the body weight of rats in different experimental groups in the embodiment of the present invention;

[0016] Figure 2 is the back crawling latency of rats in different experimental groups in the embodiment of the present invention;

[0017] Figure 3 is the number of back climbing of rats in different experimental groups in the embodiment of the present invention;

[0018] Figure 4 is the ejaculation latency of rats in different experimental groups in the embodiment of the present invention;

[0019] Figure 5 The number of ejaculations of rats in different experimental groups in the embodiment of the present invention;

[0020] Figure 6 is the sperm motility of rats in different experimental groups in the embodiment of the present invention;

[0021] Figure 7 NO content in the serum of rats in different experimental groups in the present invention;

[0022] Figure 8 The content of PDE5 in the homogenate of the rat penis in different experimental groups in the examples of the present invention;

[0023] Fig. 9 is the content of adenosine deaminase (ADA) in the homogenate of the rat penis in different experimental groups in the embodiment of the present invention;

[0024] Fig.10 The testosterone (T) content in the serum of rats in different experimental groups in the embodiment of the present invention;

[0025] Fig.11 is the follicle stimulating hormone (FSH) content in the serum of rats in different experimental groups in the embodiment of the present invention;

[0026] Fig.12 : is the luteinizing hormone (LH) content in the serum of rats in different experimental groups in the examples of the present invention. DETAILED DESCRIPTION

[0027] The present invention is further described below with reference to the examples, but the examples do not limit the present invention in any form. Unless otherwise specified, the reagents, methods and equipment used in the present invention are conventional reagents, methods and equipment in the art.

[0028] Example 1

[0029] This embodiment provides a method for preparing an extract from the leaves of the Chinese hollyhock tree, comprising the following steps:

[0030] 3 kg of leaves of the Chinese water lily tree were dried and crushed to obtain 0.9 kg. The leaves were soaked in 3 L of 85% ethanol at room temperature and then filtered to obtain the filtrate. This operation was repeated three times. The filtrates were collected and concentrated using a rotary evaporator. After freeze-vacuum drying, the ethanol extract A of the leaves of the Chinese water lily tree was obtained. The ethanol extract A of the leaves of the Chinese water lily tree was then dissolved in 1 L of water, and then extracted three times with 1 L of petroleum ether solution. The extracts were collected and concentrated using a rotary evaporator. After freeze-vacuum drying, 0.015 kg of the petroleum ether phase extract A1 of the leaves of the Chinese water lily tree was obtained.

[0031] Example 2

[0032] This embodiment provides a method for preparing an extract from the leaves of the Chinese hollyhock tree, comprising the following steps:

[0033] 5 kg of leaves of the Chinese water hyacinth were dried and crushed to obtain 1.3 kg. The leaves were soaked in 4 L of 75% ethanol at room temperature and then filtered to obtain the filtrate. This operation was repeated three times. The filtrates were collected and concentrated using a rotary evaporator. After freeze-vacuum drying, the ethanol extract B of the leaves of the Chinese water hyacinth was obtained. The ethanol extract of the leaves of the Chinese water hyacinth was then dissolved in 1.5 L of water, and then extracted three times with 1.5 L of petroleum ether solution. The extracts were collected and concentrated using a rotary evaporator. After freeze-vacuum drying, 0.023 kg of the petroleum ether phase extract B1 of the leaves of the Chinese water hyacinth was obtained.

[0034] Example 3

[0035] This embodiment provides a method for preparing an extract from the leaves of the Chinese hollyhock tree, comprising the following steps:

[0036] 8 kg of leaves of the Chinese water hyacinth were dried and crushed to obtain 2 kg. The leaves were soaked in 5 L of 80% ethanol at room temperature and then filtered to obtain the filtrate. This operation was repeated three times. The filtrates were collected and concentrated using a rotary evaporator. After freeze-vacuum drying, the ethanol extract C of the leaves of the Chinese water hyacinth was obtained. The ethanol extract of the leaves of the Chinese water hyacinth was then dissolved in 2 L of water, and then extracted three times with 2 L of petroleum ether solution. The extracts were collected and concentrated using a rotary evaporator. After freeze-vacuum drying, 0.042 kg of the petroleum ether phase extract C1 of the leaves of the Chinese water hyacinth was obtained.

[0037] 1. Inhibitory activity of extracts from the leaves of Psoralea corylifolia (ethanol extract B and petroleum ether extract B1) on PDE5A

[0038] 1. Experimental methods

[0039] Liquid scintillation counting was used to determine the 3 H-cGMP was used as a substrate to detect the inhibitory activity of PDE5, and sildenafil was selected as a positive control.

[0040] The test method is as follows: prepare Assaybuffer (1.0 mM magnesium chloride, 50 mM Tris 7.5, 1.0 mM DTT), dilute PDE5A protein with Assaybuffer and place on ice for later use, 3 H-cGMP was diluted to an appropriate concentration; the test compounds (the test compounds were ethanol extract B and petroleum ether extract B of the leaves of the Chinese water hyacinth) were dissolved and diluted with DMSO to the set concentration; 55.5 μL Assay Buffer and 2.5 μL diluted 3 H-cGMP and 2.0 μL of different concentrations of diluted test compounds, mix evenly, then add 40 μL of diluted PDE5A protein, mix gently, and react at room temperature for 15 minutes. Then add 200 μL of 0.2M ZnSO4 and 200 μL of 0.2M Ba(OH)2 to the reaction system to terminate and vortex mix. Then place the reaction tube in a high-speed centrifuge for centrifugation at a speed of 14000 rpm for 6 minutes. Take the corresponding number of scintillation tubes and add 2.5 mL of scintillation fluid for use. After the centrifugation is completed, aspirate 430 μL of the unreacted 3 The H-cGMP supernatant was added to a scintillation tube containing scintillation fluid and vortexed to mix. After sufficient shaking and mixing, the radioactivity reading was measured using a liquid scintillation counter.

[0041] Each experiment set up a substrate group (C0), a PDE5A protein group (C E ), positive control group (sildenafil, C P ) and inhibitor group (C I ), among which the substrate group (C0) only has substrate; the protein group (C E ) is substrate + protein; inhibitor group (C i ) is substrate + protein + inhibitor (test compound), and the inhibition rate of the inhibitor is given by the formula Inhibition Rate = [1-(C0-C I ) / (C0-C E )]*100% calculated. Determine the specific IC of the inhibitor 50 Value (IC 50 The inhibitor concentration at which the inhibition rate reaches 50% was set according to the results of the initial inhibition rate test, and the concentration gradient of 8-10 half-fold dilution was set. After the inhibition rate was measured, the specific IC 50 The values ​​were repeated three times independently to obtain the mean and standard deviation.

[0042] (II) Experimental results

[0043] The results of the PDE5A inhibitory activity of the extracts of the leaves of the Chinese water hyacinth in different solvents are shown in Table 1 below.

[0044] Table 1 Inhibitory activity of the extract from the leaves of the Chinese sedge tree on PDE5A

[0045]

[0046] As shown in Table 1, the ethanol extract B and the petroleum ether extract B1 of the leaves of the Chinese water hyacinth have an inhibitory activity of 2.5% and 40.6% on PDE5A at a concentration of 0.2 μg / mL, respectively. Therefore, the petroleum ether extract of the leaves of the Chinese water hyacinth has a better inhibitory effect and is more valuable for development.

[0047] 2. Effects of petroleum ether extract B1 from the leaves of Echinops sinensis on body weight and sexual behavior in rats (I) Experimental materials

[0048] 1. Animals

[0049] 64 SPF Wistar rats, 32 males and 32 females, 6 weeks old, weighing 170-180 g for males and 150-160 g for females, were purchased from Beijing Weitonglihua Experimental Animal Technology Co., Ltd. (License No.: SCXK(Qiong)2023-0031).

[0050] 2. Reagents and Drugs

[0051] Petroleum ether extract of the leaves of the Chinese water hyacinth B1 is from the leaves of the Chinese water hyacinth collected in Qiongzhong County, Hainan Province; paroxetine hydrochloride (McLean reagent); sildenafil citrate (Bidder's reagent); estradiol benzoate injection; progesterone injection (Hui Qian Fang).

[0052] 3. Preparation of solvent

[0053] Paroxetine hydrochloride solution (10 mg / kg): The drug concentration is 2 mg / mL, and DMSO is used as a cosolvent.

[0054] Solution of petroleum ether extract B1 (100 mg / kg) from the leaves of Echinops sinensis: the drug concentration was 20 mg / mL, and DMSO, PEG 300 and Tween 80 were used as cosolvents.

[0055] Sildenafil citrate solution: The drug concentration is 1 mg / mL and the cosolvent is DMSO.

[0056] (II) Experimental methods

[0057] 1. Model grouping and establishment

[0058] SPF male rats were randomly divided into 4 groups after one week of adaptive feeding, with 8 animals in each group. They were normal group (CON), paroxetine group (PAR, 10 mg / kg), paroxetine (10 mg / kg) + Chinese water lily leaf petroleum ether extract B1 group (100 mg / kg), paroxetine (10 mg / kg) + positive drug sildenafil group (SC, 5 mg / kg). Except for the normal group, the other groups were modeled at 9 am every day (subcutaneous injection of paroxetine 10 mg / kg), and the drugs were administered at 3 pm (except for the normal group and paroxetine group gavage with equal volume of pure water, the other two groups were administered accordingly), and the drugs were administered for 21 days, and the rats were weighed every 3 days.

[0059] 2. Sexual behavior experiment

[0060] Sexual behavior experiments were conducted 60 minutes after the last oral gavage. The experimental method was as follows: female rats were injected intramuscularly with 2 μg / kg estradiol benzoate injection 48 hours before mating, and 1 mg / kg progesterone injection 4 hours before mating to put the female rats in estrus. The sexual behavior experiment was conducted in a quiet room with dark red lights. First, the male rats were placed in a transparent plastic box (30×15×15 cm) for 5 minutes and recorded by a high-definition camera. From the time the female rat entered the box, the sexual behavior-related parameters of the male rats were observed and recorded within the first 30 minutes. The sexual behavior-related parameters included: ① Back-climbing latency (ML): the time required from the beginning of being in the same cage with the female rat to the first mounting; ② Back-climbing times (MF): the number of back-climbings from the first time of climbing on the female rat to ejaculation; ③ Ejaculation latency (EL): the time required from the first insertion to ejaculation; ④ Ejaculation times (EF): the number of vaginal insertions before ejaculation.

[0061] After the experiment, the weight of each group of experimental rats was weighed and recorded, and then the rats were killed. The penis, testicles, epididymis, heart, spleen, liver, kidney, and lung of the rats were taken out and accurately weighed with an electronic balance to calculate the organ coefficient.

[0062] 3. Sperm motility

[0063] The weighed left epididymis of the rat was placed in a centrifuge tube containing 1 mL of HEPES culture medium (pre-placed at room temperature), and the epididymis was cut into pieces and incubated at 37°C, 5% CO2, and saturated humidity for 20 minutes to allow the sperm to fully swim out. The sperm activity was then observed under a 200x electron microscope. The sperm motility was calculated using the following formula:

[0064] Sperm motility = (Ⅰ+Ⅱ+Ⅲ) / (Ⅰ+Ⅱ+Ⅲ+Ⅳ)×100%

[0065] Among them: Ⅰ represents the number of sperm moving forward quickly and in a straight line; Ⅱ represents the number of sperm moving forward slowly and sluggishly in a straight line or non-straight line; Ⅲ represents the number of sperm not moving forward; Ⅳ represents the number of inactive sperm.

[0066] (III) Experimental results and analysis

[0067] 1. Effects on weight gain in rats

[0068] like Figure 1 As shown, the body weights of rats in different groups gradually increased with time, with basically the same trend. It can be seen that there was no significant difference in body weight between the PAR group, PAR+SC group, PAR+100 group and the CON group, indicating that the drug is relatively safe.

[0069] 2. Effects on sexual behavior of male rats

[0070] The effect of petroleum ether extract B1 from the leaves of Echinops sinensis on the sexual behavior of male mice can be reflected by sexual behavior indexes. The sexual behavior index is one of the most intuitive and important indicators reflecting the sexual ability of experimental animals.

[0071] like Figure 2-5 As shown in the figure, it can be seen that compared with the CON group, the PAR group had a significantly increased back-climbing latency and ejaculation latency, and a reduced back-climbing frequency and ejaculation frequency. However, compared with the PAR group, the PAR+SC group and the PAR+100 group had a significantly shortened back-climbing latency and ejaculation latency, and a significantly increased back-climbing frequency and ejaculation frequency. The results of sexual behavior showed that paroxetine caused a decrease in the sexual ability of rats, while the petroleum ether extract B1 from the leaves of the Chinese water hyacinth tree could improve the sexual behavior of rats like sildenafil.

[0072] 3. Effect on sperm quality of male rats

[0073] Sperm quality can be reflected by sperm motility, viability, etc. Figure 6 As shown in the figure, compared with the CON group, the sperm motility of the rats in the PAR group was significantly decreased. Compared with the PAR group, the sperm motility of the PAR+SC group and the PAR+100 group was improved and slightly better than that of the CON group.

[0074] It can be seen that the petroleum ether extract B1 of the leaves of the Chinese water hyacinth tree can not only improve the sexual ability of rats, but also improve the sperm quality of rats.

[0075] 3. Effects of petroleum ether extract A1 from the leaves of Echinops sinensis on NO, PDE5 and ADA levels in rats

[0076] Smooth muscle relaxation is a key factor in erectile function. cGMP can cause cavernous smooth muscle and vasodilation, inducing penile erection. Nitric oxide (NO), as a signal transduction substance that causes cavernous smooth muscle relaxation, can bind to soluble guanylate cyclase (sGC), thereby promoting the expression of cGMP in the penis; PDE5 decomposes cGMP in the cavernous body, reducing smooth muscle relaxation and promoting the resolution of erection (Corbin JD. Mechanisms of action of PDE5 inhibition inerectile dysfunction. Int J Impot Res. 2004; 16.); adenosine deaminase (ADA) triggers penile erection by regulating adenosine.

[0077] 1. Experimental Materials

[0078] 1. Animals

[0079] 64 SPF Wistar rats, 32 males and 32 females, 6 weeks old, weighing 170-180 g for males and 150-160 g for females, were purchased from Beijing Weitonglihua Experimental Animal Technology Co., Ltd. (License No.: SCXK(Qiong)2023-0031).

[0080] 2. Reagents and Drugs

[0081] Petroleum ether extract of the leaves of the Chinese water hyacinth A1 was collected from the leaves of the Chinese water hyacinth in Qiongzhong County, Hainan Province; paroxetine hydrochloride (McLean reagent); sildenafil citrate (Bi De reagent); estradiol benzoate injection; progesterone injection (Hui Qian Fang); cyclic guanosine monophosphate (cGMP) ELISA kit; phosphodiesterase 5 (PDE5) ELISA kit; ADA adenosine deaminase kit (BYabscience); NO kit (Bi Yun Tian).

[0082] 3. Preparation of solvent

[0083] Paroxetine hydrochloride solution (10 mg / kg): The drug concentration is 2 mg / mL, and DMSO is used as a cosolvent.

[0084] Solution of petroleum ether extract A1 (100 mg / kg) from the leaves of Echinops sinensis: the drug concentration was 20 mg / mL, and DMSO, PEG 300 and Tween 80 were used as cosolvents.

[0085] Sildenafil citrate solution: The drug concentration is 1 mg / mL and the cosolvent is DMSO.

[0086] (II) Experimental methods

[0087] 1. Model grouping and establishment

[0088] SPF male rats were randomly divided into 4 groups after one week of adaptive feeding, with 8 animals in each group. They were normal group (CON), paroxetine group (PAR, 10 mg / kg), paroxetine (10 mg / kg) + Chinese water lily leaf petroleum ether extract A1 group (100 mg / kg), paroxetine (10 mg / kg) + positive drug sildenafil group (SC, 5 mg / kg). Except for the normal group, the other groups were modeled at 9 am every day (subcutaneous injection of paroxetine 10 mg / kg), and the drugs were administered at 3 pm (except for the normal group and paroxetine group gavage with equal volume of pure water, the other two groups were administered accordingly), and the drug administration lasted for 21 days.

[0089] 2. Sexual behavior experiment

[0090] The sexual behavior experiment was conducted 60 minutes after the last gavage. The experimental method was as follows: female rats were injected intramuscularly with 2 μg / kg estradiol benzoate injection 48 hours before mating and 1 mg / kg progesterone injection 4 hours before mating to put female rats in estrus. The sexual behavior experiment was conducted in a quiet room with dim red lights. First, male rats were placed in a transparent plastic box (30×15×15 cm) for 5 minutes and recorded by a high-definition camera.

[0091] After the sexual behavior experiment, the rats were killed, and then the rat penis was taken out. The rat penile corpus cavernosum tissue was homogenized and the contents of NO, PDE5 and ADA in the homogenate were measured.

[0092] (III) Experimental results and analysis

[0093] The results are as follows Figure 7 , Figure 8 as well as Fig. 9 As shown, from Figure 7 It can be seen that compared with the CON group, the NO level of rats in the PAR group was significantly decreased, and compared with the PAR group, the NO levels of the PAR+SC group and the PAR+100 group were significantly increased.

[0094] from Figure 8 and 9 It can be seen that compared with the CON group, the PDE5 and ADA contents of the rats in the PAR group were significantly increased, and compared with the PAR group, the PDE5 and ADA contents of the PAR+SC group and the PAR+100 group were significantly decreased, and the administration of the petroleum ether extract A1 and SC of the leaves of the Chinese water hyacinthus tree basically restored the PDE5 and ADA contents of the rats in the PAR+SC group and the PAR+100 group to the level of the CON group.

[0095] The results showed that the administration of petroleum ether extract A1 and SC from the leaves of Echinops sinensis could increase the NO content in penile tissue and reduce the content of PDE5 and ADA, thereby inducing and maintaining penile erection.

[0096] 4. Effects of petroleum ether extract C1 from the leaves of Echinops sinensis on serum sex hormones in rats

[0097] Sex hormones can stimulate sexual maturity and are indispensable in behavioral science. When sex hormone levels are disordered, it may also lead to varying degrees of erectile dysfunction. Therefore, the concentration of testosterone (T), luteinizing hormone (LH) and follicle stimulating hormone (FSH) in serum is tested to evaluate sexual ability.

[0098] 1. Experimental Materials

[0099] 1. Animals

[0100] 64 SPF Wistar rats, 32 males and 32 females, 6 weeks old, weighing 170-180 g for males and 150-160 g for females, were purchased from Beijing Weitonglihua Experimental Animal Technology Co., Ltd. (License No.: SCXK(Qiong)2023-0031).

[0101] 2. Reagents and Drugs

[0102] Petroleum ether extract of the leaves of the Chinese water hyacinth C1 is from the leaves of the Chinese water hyacinth collected in Qiongzhong County, Hainan Province; paroxetine hydrochloride (McLean reagent); sildenafil citrate (Bidder reagent); estradiol benzoate injection; progesterone injection (Hui Qianfang); testosterone (T) ELISA kit (Elabscience), luteinizing hormone (LH) ELISA kit, follicle stimulating hormone (FSH) ELISA kit.

[0103] 3. Preparation of solvent

[0104] Paroxetine hydrochloride solution (10 mg / kg): The drug concentration is 2 mg / mL, and DMSO is used as a cosolvent.

[0105] Solution of petroleum ether extract C1 (100 mg / kg) from the leaves of Echinops sinensis: the drug concentration was 20 mg / mL, and DMSO, PEG 300 and Tween 80 were selected as cosolvents.

[0106] Sildenafil citrate solution: The drug concentration is 1 mg / mL and the cosolvent is DMSO.

[0107] (II) Experimental methods

[0108] 1. Model grouping and establishment

[0109] SPF male rats were randomly divided into 4 groups after one week of adaptive feeding, with 8 animals in each group. They were normal group (CON), paroxetine group (PAR, 10 mg / kg), paroxetine (10 mg / kg) + Chinese water lily leaf petroleum ether extract C1 group (100 mg / kg), paroxetine (10 mg / kg) + positive drug sildenafil group (SC, 5 mg / kg). Except for the normal group, the other groups were modeled at 9 am every day (subcutaneous injection of paroxetine 10 mg / kg), and the drugs were administered at 3 pm (except for the normal group and paroxetine group gavage with equal volume of pure water, the other two groups were administered accordingly), and the drug administration lasted for 21 days.

[0110] 2. Sexual behavior experiment

[0111] The sexual behavior experiment was conducted 60 minutes after the last gavage. The experimental method was as follows: female rats were injected intramuscularly with 2 μg / kg estradiol benzoate injection 48 hours before mating and 1 mg / kg progesterone injection 4 hours before mating to put female rats in estrus. The sexual behavior experiment was conducted in a quiet room with dim red lights. First, male rats were placed in a transparent plastic box (30×15×15 cm) for 5 minutes and recorded by a high-definition camera.

[0112] After the sexual behavior experiment, the rats were anesthetized with isoflurane and blood was collected from the abdominal aorta. After blood collection, the blood was naturally coagulated at room temperature for 15 minutes and then at 4°C at 3000 r·min -1 After centrifugation for 15 minutes, serum was collected and the levels of T, LH and FSH in the serum were detected.

[0113] (III) Experimental results and analysis

[0114] The results are as follows Figure 10-12 As shown, from Figure 10-12 It can be seen that compared with the CON group, the concentrations of LH and FSH in the PAR group were significantly reduced. However, compared with the PAR group, the administration of the petroleum ether extract C1 and SC of the leaves of the Chinese water hyacinth tree significantly increased the concentrations of T, LH and FSH in the male rats of the PAR+SC group and the PAR+100 group.

[0115] The results showed that the treatment of petroleum ether extract C1 and SC from the leaves of the Chinese water hyacinth tree could improve the disorder of sex hormone secretion in mice, indicating that the petroleum ether extract C1 from the leaves of the Chinese water hyacinth tree has the effect of stabilizing hormone levels.

[0116] The above embodiments are preferred implementation modes of the present invention, but the implementation modes of the present invention are not limited to the above embodiments. Any other changes, modifications, substitutions, combinations, and simplifications that do not deviate from the spirit and principles of the present invention should be equivalent replacement methods and are included in the protection scope of the present invention.

Claims

1. Use of an extract from the leaves of the Chinese water hyacinth tree in the preparation of a drug for alleviating and / or treating erectile dysfunction, wherein the extract from the leaves of the Chinese water hyacinth tree is a petroleum ether phase extract of the leaves of the Chinese water hyacinth tree.

2. The use according to claim 1, characterized in that: The extract of the leaves of the Chinese hydrangea is prepared by the following method: (1) drying the leaves of the Chinese water hyacinth tree and then crushing them, soaking them in 4 to 8 times the amount of ethanol at room temperature, and then filtering to obtain a filtrate, repeating this operation at least three times, collecting and combining the filtrate, and concentrating the filtrate by reducing pressure using a rotary evaporator, and freeze-drying the ethanol extract of the leaves of the Chinese water hyacinth tree; (2) The ethanol extract of the leaves of the Chinese water hyacinth tree is dissolved in water, and extracted with a petroleum ether solution for multiple times until the extract phase is colorless, the petroleum ether extracts are combined, the pressure is reduced and concentrated using a rotary evaporator, and the freeze-dried product is the petroleum ether phase extract of the leaves of the Chinese water hyacinth tree.

3. The use according to claim 1, characterized in that: A medicine or health food having the function of relieving and / or treating erectile dysfunction and prepared with a petroleum ether extract of leaves of the Chinese water hyacinth tree as an active ingredient.

4. The use according to claim 3, characterized in that: The dosage form of the medicine or health food is capsule, pill, tablet or granule.

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