Ecological box assembly, sampling device and sampling method

Abstract

The invention discloses a plant-derived volatile substance sampling device in the technical field of ecological environment monitoring. The plant-derived volatile substance sampling device comprises a filtering assembly, an air sampler and the dynamic box assembly, the filtering assembly is connected with an air inlet of the dynamic box assembly so as to remove impurities in air input by the box body; the air extraction end of the air sampler is communicated with the air outlet of the dynamic box assembly so as to extract air in the box body; the sampling pipe is mounted on an air passage between the box body and the air sampler and is used for sampling air flowing out of the box body.

Description

Technical Field

[0001] The present invention relates to the technical field of ecological environment monitoring, and in particular to an ecological box component, a sampling device and a sampling method. Background Art

[0002] According to research, the carbon equivalent of volatile organic compounds emitted worldwide each year is approximately equivalent to global methane emissions, and is also one of the sources of ozone in the atmosphere, which is closely related to climate warming and environmental quality. Plant-derived volatile substances are mostly organic substances such as various hydrocarbons, and volatile organic substances released by vegetation ecosystems account for the vast majority of global volatile organic compound emissions. Therefore, collecting plant-derived volatile substances and conducting research on them has positive practical significance.

[0003] The collection of volatile substances from plants requires the elimination of interference from large and small particles and volatile impurities in the air. Therefore, a box or static box is generally used to cover the plants, and then the gas in the box is extracted for collection.

[0004] In the prior art, for plants with tall branches, it is easier to seal the plants to eliminate interference from external air impurities during collection. For example, CN114166586A discloses a device for sampling plant volatile organic compounds, which uses a flexible gas enrichment bag to cover a branch of the plant far from the ground, making it easier to achieve sealing. However, for plants with shorter bodies and close to the ground, it is difficult to achieve sealing of the box due to the flatness of the ground and the influence of vegetation on the ground. Summary of the invention

[0005] The purpose of the present invention is to provide an ecological box assembly, a sampling device and a sampling method to solve the technical problem in the prior art that it is difficult to achieve box sealing for plants that are shorter and close to the ground due to the flatness of the ground and the influence of vegetation on the ground.

[0006] In order to solve the above technical problems, the present invention specifically provides a dynamic box assembly, including a box body, a base and an annular blade;

[0007] The base is arranged on the surface of the plant growth area to demarcate the plant to be sampled;

[0008] The box is arranged above the base, and the connection between the box and the base is sealed to prevent outside air from entering the box through the gap between the base and the box;

[0009] The annular blade can penetrate into the soil, and the connection between the annular blade and the base is sealed to prevent external air from entering the box from the gap between the base and the soil surface.

[0010] As a preferred solution of the present invention, an upper annular groove is provided on the upper portion of the base, and the lower edge of the box body can be inserted into the upper annular groove;

[0011] The upper annular groove is filled with a solution to prevent external air from entering the box through the gap between the base and the box; or,

[0012] An upper air bag is arranged between the outer wall of the box body and the outer groove wall of the upper annular groove to prevent external air from entering the box body through the gap between the base and the box body.

[0013] As a preferred solution of the present invention, a lower annular groove is provided below the base, the inner wall of the annular blade is in contact with the inner groove wall of the lower annular groove, and a filling gap exists between the outer wall of the annular blade and the outer groove wall of the lower annular groove;

[0014] A lower air bag is arranged in the filling gap to prevent external air from entering the box body from the gap between the base and the soil surface.

[0015] As a preferred solution of the present invention, the height of the annular blade is 1.5-2 times the depth of the lower annular groove, and the thickness of the annular blade is 0.2-0.3 times the width of the lower annular groove.

[0016] As a preferred solution of the present invention, a force-bearing plate extending horizontally outward is provided at the upper end of the annular blade.

[0017] As a preferred solution of the present invention, a fan is installed in the box to allow the plant-derived volatile substances to be fully and evenly mixed in the space inside the box.

[0018] In order to solve the above technical problems, the present invention further provides a sampling device for plant-derived volatile substances, comprising a filter assembly, an air sampler and the above dynamic box assembly;

[0019] The filter assembly is connected to the air inlet of the dynamic box assembly to remove impurities in the air input from the box;

[0020] The air extraction end of the air sampler is connected to the air outlet of the dynamic box assembly to extract the air in the box;

[0021] The sampling tube is installed on the airway between the box and the air sampler, and is used to sample the air flowing out of the box.

[0022] As a preferred solution of the present invention, the filter assembly comprises a filter head, a MnO2 filter and an activated carbon filter arranged in sequence according to the air flow direction;

[0023] The filter head is used to filter larger impurities, the MnO2 filter is used to remove ozone, and the activated carbon filter is used to remove small and medium-sized particles and volatile impurities.

[0024] In order to solve the above technical problems, the present invention further provides a method for sampling volatile substances from plants, using the above sampling device, comprising the following steps:

[0025] Install and connect the filter assembly, dynamic box assembly and air sampler without connecting the sampling tube yet;

[0026] Start the air sampler and run it for 10 minutes with the flow rate set to 1000ml / min to replace the air in the box;

[0027] Afterwards, the sampling tube was installed on the airway between the box and the air sampler, and the air sampler was started to sample for 30 minutes. During the sampling period, the air sampler flow rate was set to 200 ml / min.

[0028] During sampling, the box's fan keeps turning to mix the gas in the box;

[0029] After sampling, remove the sampling tube, put it into a vacuum bag, and refrigerate it in the refrigerator.

[0030] As a preferred solution of the present invention, the installation method of the dynamic box assembly is:

[0031] The lower air bag is installed in the lower annular groove of the base, and the airtight core of the lower air bag passes through the circular hole on the lower annular groove to the outside of the base;

[0032] Place the annular blade on the ground, fit the lower annular groove into the annular blade, and insert the upper part of the annular blade into the lower annular groove along the inner groove wall of the lower annular groove;

[0033] Press down the base so that the lower part of the annular blade is inserted into the soil;

[0034] Inflate the lower air bag so that the outer surface of the lower air bag is in close contact with the groove wall and the annular blade of the lower annular groove, so as to prevent the outside air from entering the box from the gap between the base and the soil surface;

[0035] The box body is placed in the upper annular groove, and water is poured into the upper annular groove or an upper air bag is used to tightly fit the upper annular groove and the box body to prevent external air from entering the box body through the gap between the base and the box body.

[0036] Compared with the prior art, the present invention has the following beneficial effects:

[0037] The annular blade of the dynamic box assembly can penetrate into the soil, and the connection between the annular blade and the base can be sealed, which can prevent outside air from entering the box from the gap between the base and the soil surface; it is suitable for uneven and lushly vegetated ground, and it is easy to achieve sealing of the box when sampling plants with shorter bodies and close to the ground. BRIEF DESCRIPTION OF THE DRAWINGS

[0038] In order to more clearly illustrate the implementation methods of the present invention or the technical solutions in the prior art, the following briefly introduces the drawings required for the implementation methods or the description of the prior art. Obviously, the drawings in the following description are only exemplary, and for ordinary technicians in this field, other implementation drawings can be derived from the provided drawings without creative work.

[0039] Figure 1 It is a schematic diagram of the structure of the sampling device in the present invention;

[0040] Figure 2 It is a structural schematic diagram of an implementation mode of the base and the annular blade in the present invention;

[0041] Figure 3 for Figure 2 A cross-sectional schematic diagram of

[0042] Figure 4 It is a structural schematic diagram of another embodiment of the base and the annular blade in the present invention;

[0043] Figure 5 for Figure 4 A cross-sectional schematic diagram of

[0044] Figure 6 for Figure 5 Enlarged schematic diagram of the right part.

[0045] The numbers in the figure represent the following:

[0046] 1-box, 2-base, 3-annular blade, 4-upper annular groove, 5-upper air bag, 6-lower annular groove, 7-lower air bag, 8-fan, 9-air sampler, 10-sampling tube, 11-filter head, 12-MnO2 filter, 13-activated carbon filter. DETAILED DESCRIPTION

[0047] The following will be combined with the drawings in the embodiments of the present invention to clearly and completely describe the technical solutions in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0048] like Figures 2 to 6 As shown, the present invention specifically provides a dynamic box assembly, including a box body 1, a base 2 and an annular blade 3;

[0049] The base 2 is arranged on the surface of the plant growth area to demarcate the plant to be sampled;

[0050] The box body 1 is arranged above the base 2, and the connection between the box body 1 and the base 2 is sealed to prevent outside air from entering the box body 1 through the gap between the base 2 and the box body 1;

[0051] The annular blade 3 can penetrate into the soil, and the connection between the annular blade 3 and the base 2 is sealed to prevent external air from entering the box body 1 from the gap between the base 2 and the soil surface.

[0052] The annular blade 3 of the dynamic box assembly can penetrate into the soil, and the connection between the annular blade 3 and the base 2 can be sealed, which can prevent outside air from entering the box body 1 from the gap between the base 2 and the soil surface; it is suitable for uneven and lushly vegetated ground, and it is easy to achieve the sealing of the box body 1 when sampling plants with shorter bodies and close to the ground.

[0053] Furthermore, the present invention provides two embodiments for the connection between the base 2 and the box 1, as follows:

[0054] An upper annular groove 4 is provided on the upper portion of the base 2, and the lower edge of the box body 1 can be inserted into the upper annular groove 4;

[0055] like Figure 2 and Figure 3 As shown, the upper annular groove 4 is filled with a solution, which can be water. The water in the upper annular groove 4 submerges the lower edge of the box body 1 to achieve water sealing to prevent external air from entering the box body 1 through the gap between the base 2 and the box body 1;

[0056] Or, if Figure 4 and Figure 5 As shown, an upper airbag 5 is arranged between the outer wall of the box body 1 and the outer groove wall of the upper annular groove 4. The upper airbag 5 is inflated so that the outer wall of the upper airbag 5 fits with the groove wall of the upper annular groove 4 and the outer wall of the box body 1 respectively, so as to prevent outside air from entering the box body 1 through the gap between the base 2 and the box body 1.

[0057] Furthermore, the present invention realizes the sealing between the base 2 and the annular blade 3 in the following manner.

[0058] A lower annular groove 6 is provided below the base 2, and the inner wall of the annular blade 3 is fitted with the inner groove wall of the lower annular groove 6, that is, the lower annular groove 6 can limit the annular blade 3 to prevent the annular blade 3 from shaking left and right in the lower annular groove 6, and there is a filling gap between the outer wall of the annular blade 3 and the outer groove wall of the lower annular groove 6;

[0059] A lower air bag 7 is arranged in the filling gap, and a hole is also arranged on the base 2 for the airtight core of the lower air bag 7 to pass through. By inflating the lower air bag 7, the outer surface of the lower air bag 7 is closely attached to the groove wall of the lower annular groove 6 and the annular blade 3, so as to prevent outside air from entering the box body 1 from the gap between the base 2 and the soil surface.

[0060] Furthermore, the height of the annular blade 3 is 1.5-2 times the depth of the lower annular groove 6 , and the thickness of the annular blade 3 is 0.2-0.3 times the width of the lower annular groove 6 .

[0061] Furthermore, since the annular blade 3 is relatively thin as a whole, in the process of pressing the base 2 down to penetrate the annular blade 3 into the soil, in order to prevent the upper end of the annular blade 3 from deforming and bending under the extrusion of the pressing base 2, the upper end of the annular blade 3 is provided with a force-bearing plate 14 extending horizontally outward, which can increase the force-bearing area.

[0062] Furthermore, a fan 8 is installed in the box 1 to allow the plant-derived volatile substances to be fully and evenly mixed in the space inside the box 1 .

[0063] like Figure 1 As shown, in order to solve the above technical problems, the present invention further provides a sampling device for plant-derived volatile substances, comprising a filter assembly, an air sampler 9 and the above dynamic box assembly;

[0064] The filter assembly is connected to the air inlet of the dynamic box assembly to remove impurities in the air input from the box body 1;

[0065] The air extraction end of the air sampler 9 is connected to the air outlet of the dynamic box assembly to extract the air in the box body 1;

[0066] The sampling tube 10 is installed on the air passage between the box 1 and the air sampler 9 and is used to sample the air flowing out of the box 1 .

[0067] The method of sampling using the above-mentioned sampling device is as follows:

[0068] Install and connect the filter assembly, dynamic box assembly and air sampler 9, but do not connect the sampling tube 10 first;

[0069] Start the air sampler 9 and run it for 10 minutes with the flow rate set to 1000 ml / min to replace the air in the box 1;

[0070] Afterwards, the sampling tube 10 is installed on the airway between the box 1 and the air sampler 9, and the air sampler 9 is started to sample for 30 minutes. During the sampling period, the flow rate of the air sampler 9 is set to 200 ml / min;

[0071] During the sampling period, the fan 8 of the box 1 keeps rotating to mix the gas in the box 1;

[0072] After the sampling is completed, the sampling tube 10 is removed, placed in a vacuum bag, and refrigerated in a refrigerator.

[0073] Further, the filter assembly includes a filter head 11, a MnO2 filter 12 and an activated carbon filter 13 which are arranged in sequence according to the air flow direction;

[0074] The filter head 11 is used to filter larger impurities, the MnO2 filter 12 is used to remove ozone, and the activated carbon filter 13 is used to remove small and medium-sized particles and volatile impurities.

[0075] As a preferred solution of the present invention, the installation method of the dynamic box assembly is:

[0076] The lower airbag 7 is installed in the lower annular groove 6 of the base 2, and the airtight core of the lower airbag 7 passes through the circular hole on the lower annular groove 6 to the outside of the base 2;

[0077] Place the annular blade 3 on the ground, fit the lower annular groove 6 into the annular blade 3, and insert the upper portion of the annular blade 3 into the lower annular groove 6 along the inner groove wall of the lower annular groove 6;

[0078] Press down the base 2 so that the lower part of the annular blade 3 is inserted into the soil;

[0079] Inflate the lower airbag 7 so that the outer surface of the lower airbag 7 is in close contact with the groove wall of the lower annular groove 6 and the annular blade 3 to prevent external air from entering the box body 1 from the gap between the base 2 and the soil surface;

[0080] Place the box body 1 in the upper annular groove 4, and inject water into the upper annular groove 4 or use the upper air bag 5 to fit the upper annular groove 4 and the box body 1 tightly, so as to prevent external air from entering the box body 1 through the gap between the base 2 and the box body 1.

[0081] The above embodiments are only exemplary embodiments of the present application and are not intended to limit the present application. The protection scope of the present application is defined by the claims. Those skilled in the art may make various modifications or equivalent substitutions to the present application within the essence and protection scope of the present application, and such modifications or equivalent substitutions shall also be deemed to fall within the protection scope of the present application.

Claims

1. A dynamic box assembly, characterized in that: It comprises a box body (1), a base (2) and an annular blade (3); The base (2) is arranged on the surface of the plant growth area to demarcate the plant to be sampled; The box body (1) is arranged above the base (2), and the connection between the box body (1) and the base (2) is sealed to prevent outside air from entering the box body (1) through a gap between the base (2) and the box body (1); The annular blade (3) can penetrate into the soil, and the connection between the annular blade (3) and the base (2) is sealed to prevent external air from entering the box (1) through the gap between the base (2) and the soil surface.

2. A dynamic box assembly according to claim 1, characterized in that: An upper annular groove (4) is provided on the upper part of the base (2), and the lower edge of the box body (1) can be inserted into the upper annular groove (4); The upper annular groove (4) is filled with a solution to prevent external air from entering the box body (1) through the gap between the base (2) and the box body (1); or, An upper air bag (5) is provided between the outer wall of the box body (1) and the outer groove wall of the upper annular groove (4) to prevent external air from entering the box body (1) through the gap between the base (2) and the box body (1).

3. A dynamic box assembly according to claim 2, characterized in that: A lower annular groove (6) is provided below the base (2), the inner wall of the annular blade (3) is in contact with the inner groove wall of the lower annular groove (6), and a filling gap exists between the outer wall of the annular blade (3) and the outer groove wall of the lower annular groove (6); A lower air bag (7) is arranged in the filling gap to prevent external air from entering the box body (1) from the gap between the base (2) and the soil surface.

4. A dynamic box assembly according to claim 3, characterized in that: The height of the annular blade (3) is 1.5-2 times the depth of the lower annular groove (6), and the thickness of the annular blade (3) is 0.2-0.3 times the width of the lower annular groove (6).

5. A dynamic box assembly according to claim 4, characterized in that: The upper end of the annular blade (3) is provided with a force-bearing plate (14) extending horizontally outward.

6. A dynamic box assembly according to claim 1, characterized in that: A fan (8) is installed in the box (1) to ensure that the plant-derived volatile substances are fully and evenly mixed in the space inside the box (1).

7. A sampling device for volatile substances from plants, characterized in that: It comprises a filter assembly, an air sampler (9) and a dynamic box assembly as claimed in any one of claims 1 to 6; The filter assembly is connected to the air inlet of the dynamic box assembly to remove impurities in the air input from the box body (1); The air extraction end of the air sampler (9) is in communication with the air outlet of the dynamic box assembly so as to extract the air in the box body (1); The sampling tube (10) is installed on the air passage between the box (1) and the air sampler (9) and is used to sample the air flowing out of the box (1).

8. The sampling device for plant-derived volatile substances according to claim 7, characterized in that: The filter assembly comprises a filter head (11), a MnO2 filter (12) and an activated carbon filter (13) which are arranged in sequence according to the air flow direction; The filter head (11) is used to filter larger impurities, the MnO2 filter (12) is used to remove ozone, and the activated carbon filter (13) is used to remove small and medium-sized particles and volatile impurities.

9. A method for sampling volatile substances from plants, characterized in that: Using the sampling device according to claim 7 or 8 comprises the following steps: Install and connect the filter assembly, dynamic box assembly and air sampler (9), without connecting the sampling tube (10); The air sampler (9) is started and operated for 10 minutes with the flow rate set to 1000 ml / min to replace the air in the box (1); Afterwards, the sampling tube (10) is installed on the airway between the box (1) and the air sampler (9), and the air sampler (9) is started to sample for 30 minutes. During the sampling period, the flow rate of the air sampler (9) is set to 200 ml / min; During sampling, the fan (8) of the box (1) keeps rotating to mix the gas in the box (1); After sampling, remove the sampling tube (10), put it into a vacuum bag, and place it in a refrigerator for refrigeration.

10. The method for sampling volatile substances from plants according to claim 9, characterized in that: The installation method of the dynamic box component is: A lower air bag (7) is installed in the lower annular groove (6) of the base (2), and the airtight core of the lower air bag (7) passes through the circular hole on the lower annular groove (6) to the outside of the base (2); The annular blade (3) is placed on the ground, and the lower annular groove (6) is engaged with the annular blade (3), so that the upper part of the annular blade (3) is inserted into the lower annular groove (6) along the inner groove wall of the lower annular groove (6); Pressing down the base (2) so that the lower part of the annular blade (3) is inserted into the soil; Inflate the lower air bag (7) so that the outer surface of the lower air bag (7) is in close contact with the groove wall of the lower annular groove (6) and the annular blade (3), so as to prevent external air from entering the box body (1) through the gap between the base (2) and the soil surface; The box body (1) is placed in the upper annular groove (4), and water is poured into the upper annular groove (4) or an upper air bag (5) is used to closely contact the upper annular groove (4) and the box body (1) to prevent external air from entering the box body (1) through the gap between the base (2) and the box body (1).

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