Method for breeding epinephelus fuscoguttatus

Abstract

The invention discloses a method for breeding epinephelus fuscoguttatus, and the method achieves the breeding of epinephelus fuscoguttatus through the laying of a fish pond, the pretreatment of the fish pond, the water quality management, the throwing of larva fish, the bait management and the water quality replacement. According to the method, the ecological environment in the nursery pond can be well maintained through the optimized matching of the feed components of the epinephelus fuscoguttatus fry in different growth stages, the requirements of the epinephelus fuscoguttatus fries for nutrition and feeding capacity in different stages are met, the death rate of juvenile fishes is effectively reduced, and therefore higher economic benefits are brought.

Description

Technical Field

[0001] The invention relates to the technical field of aquaculture, and in particular to a method for raising seedlings of brown-spotted grouper. Background Art

[0002] The body of the brown-spotted grouper is oblong, flat and sturdy. The dorsal frame of the adult fish has a notch at the eye, and there is a distinct bulge from that point to the starting point of the dorsal fin. The interorbital area is flat or slightly concave. The eyes are small and shorter than the snout. The mouth is large, with small canines or none at the front of the upper and lower jaws, and the teeth on both sides are fine and pointed. There are about 3 or 4 rows of teeth in the middle and lateral part of the lower jaw. The inner teeth are about twice as long as the outer teeth. The canine teeth are difficult to detect, and the upper jaw extends forward to the eye. The preoperculum is round and has small serrations. The upper edge of the gill cover is obviously convex and almost perpendicular to the posterior edge of the gill cover. The front edge of the preorbital bone is deeply concave below the nostril. The posterior nostril is triangular, 4 to 7 times the size of the anterior nostril in adult fish, and the two nostrils are closed. The fish's lateral line scales are smooth and have auxiliary scales. The number of lateral line scale holes is 52 to 58, and the number of vertical row scales is 102 to 115. The body is light yellow-brown, with 5 vertical series of dark brown spots forming irregular stripes. The head, sides of the body and fins are densely covered with small brown spots. The small spots on the dark spots are much darker than the small spots between the dark spots. The posterior edge of the caudal peduncle has a fuzzy black saddle-shaped spot, and there are 2 or 3 fuzzy dark stripes on one side of the jaw.

[0003] Brown-spotted grouper (Epinephelus fuscoguttatus) is a fish belonging to the genus Epinephelus of the family Serranidae in the order Perciformes. It is widely distributed in tropical and subtropical waters of the Indian and Pacific Oceans. In China, it is mainly distributed in southern, western, northeastern Taiwan and the Penghu region. This fish is one of the main targets of grouper hybrid breeding. It has been hybridized with the saddle-banded grouper to produce the "Pearl Gentian", which shows its important position in grouper farming and breeding. Brown-spotted grouper has special requirements for the ecological environment. In order to improve the survival rate of brown-spotted grouper seedlings, accelerate the reproduction rate of brown-spotted grouper, and fully develop the species of brown-spotted grouper, it is particularly necessary to develop a seedling breeding method suitable for brown-spotted grouper. Summary of the invention

[0004] In view of the above-mentioned defects of the prior art, the present invention proposes a method for raising seedlings of brown-spotted grouper to solve the problems raised by the above-mentioned background technology.

[0005] In order to achieve the above object, the present invention provides the following technical solutions:

[0006] A method for raising brown-spotted grouper fry comprises the following steps:

[0007] S1. Fish pond laying: Set up an indoor rectangular cement pool, clean the surroundings of the cement pool, disinfect it with quicklime before raising seedlings, and dry it for later use; shovel in sea mud and sea sand with a weight ratio of 5-9:1-3 to cover the bottom of the cement pool, and compact it to a height of 8-12 cm, then plant submerged plants that account for 10-15% of the total area of ​​the cement pool, and set a drainage outlet at the bottom of the pool;

[0008] S2, fish pond pretreatment: pour seawater into the fish pond to a height of 80-100 cm, and then sprinkle the Bacillus fermentation liquid accounting for 0.5-1.5% of the total volume of seawater;

[0009] S3. Water quality management: adjust various parameters in the fish pond, water salinity 20-25‰, pH 5.5-6.5, dissolved oxygen 4-8mg / L, water temperature 22-25℃, nitrite content 0.050-0.080mg / L, water surface light intensity 2000-2800Lx;

[0010] S4. Release fry: Release hatched fry into the fish pond at a density of 1,000 to 1,500 fry / m 2 ;

[0011] S5. Bait management:

[0012] Early stage: on the 1st to 20th day after the fry enter the pond, artificial bait A is fed into the fish pond 3 to 5 times a day, and the feeding amount each time is 3 to 5% of the weight of the fry; the artificial bait A comprises the following raw materials in parts by weight: 20 to 35 parts of earthworm powder, 10 to 15 parts of Cyperus esculentu, 7 to 10 parts of Basil extract (Ocimum basilicum L.), 5 to 8 parts of Trifolium pratense L., 1 to 4 parts of Sarsaparilla extract (Smilax spp.) and 50 to 70 parts of water;

[0013] The middle stage: on the 21st to 30th day after the fry enter the pond, artificial feed B is fed into the fish pond 2 to 4 times a day, and the feeding amount each time is 6 to 10% of the weight of the fry; the artificial feed B comprises the following raw materials by weight: 15 to 25 parts of kelp powder, 10 to 20 parts of soybean meal, 8 to 15 parts of leech powder, 5 to 10 parts of catechu powder (Catechupowder (Acaciacatechu Willd.)), 2 to 6 parts of beet red and 30 to 45 parts of water;

[0014] The method comprises feeding artificial feed C to the fish pond 30 days after the fry enter the pond, feeding 1 to 3 times a day, and each feeding amount is 12 to 15% of the weight of the fry; the artificial feed C comprises the following raw materials in parts by weight: 12 to 30 parts of rapeseed cake, 12 to 22 parts of alfalfa (Alfalfa extract (Medicagosativa L.)), 10 to 15 parts of cinnamaldehyde, 8 to 15 parts of phloretin, 5 to 9 parts of lemon balm (leaves of Melissa officinalis L., a plant of the Labiatae family), 3 to 7 parts of valerian (Valerian root extract (Valeriana officinalis L.)), 1 to 5 parts of red raspberry enzyme and 35 to 45 parts of water;

[0015] S6. Water quality replacement: After the fry enter the pond, the seawater should be replaced every 7 to 10 days, and the amount of water replaced is 10 to 15% of the original pond water volume.

[0016] Preferably, red raspberry enzyme is purchased from Shandong Tiansui Biotechnology Co., Ltd., production license number SC10637150100152.

[0017] Preferably, the submerged plants in step S1 are Elodea, Psoralea corylifolia and Potamogeton crispus, which are planted at a planting area ratio of 1:(3-7):(1-8).

[0018] Preferably, the preparation method of the bacillus fermentation broth is as follows: adding wheat bran and sucrose to seawater, stirring to obtain a mixture, inoculating Bacillus polymyxa with a concentration of 3.0×107 to 5.0×109 CFU / mL at a ratio of 1.5 to 2% of the total mass of the mixture, aerobically fermenting for 48 to 72 hours at a temperature of 30 to 35°C to obtain a fermentation product; centrifuging the fermentation product and taking the supernatant, which is the bacillus fermentation broth.

[0019] Preferably, seawater, wheat bran and sucrose are mixed in a mass ratio of (60-80):(6-9):1.

[0020] Preferably, Paenibacillus polymyxa is purchased from Hubei Qiniu Chemical Technology Co., Ltd.

[0021] Preferably, the preparation method of artificial bait A comprises the following steps:

[0022] (1) adding β-mannanase to basil, red clover and smilax china and fermenting them for 3 to 7 days at a temperature of 55 to 65° C. to obtain a Chinese medicine fermentation product;

[0023] (2) Mixing and crushing the cyperus rotundus and the fermented Chinese medicine, adding earthworm powder and water and mixing evenly to prepare the raw material of artificial bait A;

[0024] (3) The artificial bait A raw material is granulated by a spray drying method to obtain artificial bait A.

[0025] Preferably, the artificial bait A comprises the following raw materials in parts by weight: 30 parts of earthworm meal, 12 parts of Cyperus oleifera, 8 parts of basil, 7 parts of Trifolium pratense, 3 parts of Smilax china and 60 parts of water.

[0026] Preferably, the artificial bait B comprises the following raw materials in parts by weight: 20 parts of kelp powder, 15 parts of soybean meal, 10 parts of leech powder, 8 parts of catechu powder, 4 parts of beetroot red and 40 parts of water.

[0027] Preferably, the artificial bait C comprises the following raw materials in parts by weight: 18 parts of rapeseed cake, 18 parts of alfalfa, 11 parts of cinnamaldehyde, 10 parts of phloretin, 8 parts of lemon balm, 6 parts of valerian, 4 parts of red raspberry enzymes and 40 parts of water.

[0028] Compared with the prior art, the present invention has the following beneficial effects:

[0029] (1) The present invention arranges sea mud, sea sand and plants submerged plants at the bottom of the fish pond to simulate the growth environment of wild brown-spotted grouper to the maximum extent, create a better ecological environment, and improve the quality of fish meat; submerged plants can absorb nutrients such as nitrogen and phosphorus in the water to promote the development of microbial communities, thereby achieving the purpose of purifying water quality; at the same time, submerged plants are rich in cellulose and lignin, providing a habitat and food source for brown-spotted grouper.

[0030] (2) Different types of aquatic plants have different characteristics. The present invention finally selects the combination of Elodea + yellow silk grass + water chestnut to effectively improve the yield and quality of brown-spotted grouper.

[0031] (3) Through the flocculation effect of Bacillus, the residual bait, bacterial protein or other microorganisms in the water are converted into edible flocs. Through the bacteria themselves, cell walls or other metabolites such as enzymes and polysaccharides, the non-specific immune system of brown spot grouper is stimulated and activated, thereby improving its body immunity.

[0032] (4) By selecting different artificial feeds at different growth stages, not only can the immunity of fry be improved at different stages, but also the nutrients required for growth can be fully provided, reducing the disease and death of fry, and effectively reducing the cannibalism caused by the demand for feed intake in the later stage of fry, thereby improving the survival rate of young fish. DETAILED DESCRIPTION

[0033] The technical solution of the present invention is further described in detail below in conjunction with specific implementation methods.

[0034] Example 1

[0035] A method for raising brown-spotted grouper fry comprises the following steps:

[0036] S1. Fish pond laying: Set up an indoor rectangular cement pool, clean the surroundings of the cement pool, disinfect it with quicklime before raising seedlings, and dry it for later use; shovel in sea mud and sea sand in a weight ratio of 5:1 to cover the bottom of the cement pool, and the height after compaction is 8 cm, and then plant submerged plants that account for 10% of the total area of ​​the cement pool, and set a drainage outlet at the bottom of the pool; the submerged plants are Elodea, yellow silk grass and water chestnut, which are planted at a planting area ratio of 1:3:1;

[0037] S2, fish pond pretreatment: pour seawater into the fish pond to a height of 80 cm, and then sprinkle the Bacillus fermentation liquid accounting for 0.5% of the total volume of the seawater; the preparation method of the Bacillus fermentation liquid is to add wheat bran and sucrose to the seawater, stir evenly to obtain a mixture, and inoculate at a concentration of 3.0×10 7 CFU / mL of Paenibacillus polymyxa is aerobically fermented at a temperature of 30°C for 48 hours to obtain a fermentation product; the fermentation product is centrifuged and the supernatant is taken, which is the Bacillus fermentation liquid; wherein seawater, wheat bran and sucrose are mixed in a mass ratio of 60:6:1;

[0038] S3. Water quality management: adjust various parameters in the fish pond, water salinity 20‰, pH 5.5, dissolved oxygen 4mg / L, water temperature 22℃, nitrite content 0.050mg / L, water surface light intensity 2000Lx;

[0039] S4. Release fry: Release the hatched fry into the fish pond at a density of 1,000 fry / m 2 ;

[0040] S5. Bait management:

[0041] Early stage: on the 1st to 20th day after the fry enter the pond, artificial bait A is fed into the fish pond 3 times a day at 09:00, 13:00 and 18:00, and the feeding amount each time is 3% of the weight of the fry; the artificial bait A comprises the following raw materials by weight: 20 parts of earthworm powder, 10 parts of cyperus rotundus, 7 parts of basil, 5 parts of clover, 1 part of sarsaparilla and 50 parts of water;

[0042] The middle stage: on the 21st to 30th day after the fry enter the pond, feed the artificial feed B twice a day at 09:00 and 18:00, and the feeding amount each time is 6% of the weight of the fry; the artificial feed B comprises the following raw materials by weight: 15 parts of kelp powder, 10 parts of soybean meal, 8 parts of leech powder, 5 parts of catechu powder, 2 parts of beet red and 30 parts of water;

[0043] The later stage: 30 days after the fry enter the pond, feeding the fish pond with artificial feed C, once a day, at 09:00, and each feeding amount is 12% of the weight of the fry; the artificial feed C comprises the following raw materials by weight: 12 parts of rapeseed cake, 12 parts of alfalfa, 10 parts of cinnamaldehyde, 8 parts of phloretin, 5 parts of lemon balm, 3 parts of valerian, 1 part of red raspberry enzyme and 35 parts of water;

[0044] S6. Water quality replacement: After the fry enter the pond, the seawater is replaced every 7 days, and the amount of water replaced is 10% of the original fish pond water volume.

[0045] The preparation method of the artificial bait A comprises the following steps:

[0046] (1) adding β-mannanase to basil, red clover and smilax china and fermenting them for 3 days at a temperature of 55° C. to obtain a Chinese medicine fermentation product;

[0047] (2) Mixing and crushing the cyperus rotundus and the fermented Chinese medicine, adding earthworm powder and water and mixing evenly to prepare the raw material of artificial bait A;

[0048] (3) The raw material of artificial bait A is granulated by spray drying, using a spray gun type spray dryer, the temperature is 100° C., the spray gun pressure is 1.0 MPa, and the spray gun diameter is 0.8 mm, so as to obtain artificial bait A.

[0049] Example 2

[0050] A method for raising brown-spotted grouper fry comprises the following steps:

[0051] S1. Fish pond laying: Set up an indoor rectangular cement pool, clean the surroundings of the cement pool, disinfect it with quicklime before raising seedlings, and dry it for later use; shovel in sea mud and sea sand in a weight ratio of 9:3 to cover the bottom of the cement pool, and the height after compaction is 12 cm, and then plant submerged plants that account for 15% of the total area of ​​the cement pool, and set a drainage outlet at the bottom of the pool; the submerged plants are Elodea, yellow silk grass and water chestnut, which are planted at a planting area ratio of 1:7:8;

[0052] S2. Fish pond pretreatment: seawater is poured into the fish pond to a height of 100 cm, and then a Bacillus fermentation liquid accounting for 1.5% of the total volume of the seawater is splashed; the preparation method of the Bacillus fermentation liquid is to add wheat bran and sucrose to the seawater, stir evenly to obtain a mixture, and inoculate at a concentration of 5.0×10 9 CFU / mL of Paenibacillus polymyxa is aerobically fermented at a temperature of 35°C for 72 hours to obtain a fermentation product; the fermentation product is centrifuged and the supernatant is taken, which is the Bacillus fermentation liquid; wherein seawater, wheat bran and sucrose are mixed in a mass ratio of 80:9:1;

[0053] S3. Water quality management: adjust various parameters in the fish pond, water salinity 25‰, pH 6.5, dissolved oxygen 8mg / L, water temperature 25℃, nitrite content 0.080mg / L, water surface light intensity 2800Lx;

[0054] S4. Release fry: Release hatched fry into the fish pond at a density of 1500 fry / m 2 ;

[0055] S5. Bait management:

[0056] Early stage: on the 1st to 20th day after the fry enter the pond, artificial bait A is fed into the fish pond 5 times a day, the feeding time points are 09:00, 13:00, 16:00, 18:00 and 21:00, and the feeding amount each time is 5% of the weight of the fry; the artificial bait A comprises the following raw materials by weight: 35 parts of earthworm powder, 15 parts of Cyperus rotundus, 10 parts of basil, 8 parts of Trifolium pratense, 4 parts of Smilax china and 70 parts of water;

[0057] The middle stage: on the 21st to 30th day after the fry enter the pond, feed the artificial feed B to the fish pond 4 times a day, at 09:00, 13:00, 18:00 and 21:00, and the feeding amount each time is 10% of the weight of the fry; the artificial feed B comprises the following raw materials by weight: 25 parts of kelp powder, 20 parts of soybean meal, 15 parts of leech powder, 10 parts of catechu powder, 6 parts of beet red and 45 parts of water;

[0058] Later stage: 30 days after the fry enter the pond, feeding artificial feed C to the fish pond, feeding 3 times / day, feeding time points are 09:00, 13:00 and 18:00, and each feeding amount is 15% of the weight of the fry; the artificial feed C comprises the following raw materials by weight: 30 parts of rapeseed cake, 22 parts of alfalfa, 15 parts of cinnamaldehyde, 15 parts of phloretin, 9 parts of lemon balm, 7 parts of valerian, 5 parts of red raspberry enzyme and 45 parts of water;

[0059] S6. Water quality replacement: After the fry enter the pond, the seawater should be replaced every 10 days, and the amount of water replaced is 15% of the original fish pond water volume.

[0060] The preparation method of the artificial bait A comprises the following steps:

[0061] (1) adding β-mannanase to basil, red clover and smilax china and fermenting them for 7 days at a temperature of 65° C. to obtain a Chinese medicine fermentation product;

[0062] (2) Mixing and crushing the cyperus rotundus and the fermented Chinese medicine, adding earthworm powder and water and mixing evenly to prepare the raw material of artificial bait A;

[0063] (3) The raw material of artificial bait A is granulated by spray drying, using a spray gun type spray dryer, the temperature is 100° C., the spray gun pressure is 1.0 MPa, and the spray gun diameter is 0.8 mm, so as to obtain artificial bait A.

[0064] Example 3

[0065] A method for raising brown-spotted grouper fry comprises the following steps:

[0066] S1. Fish pond laying: Set up an indoor rectangular cement pool, clean the surroundings of the cement pool, disinfect it with quicklime before raising seedlings, and dry it for later use; shovel in sea mud and sea sand in a weight ratio of 7:2 to cover the bottom of the cement pool, and compact it to a height of 10 cm, then plant submerged plants that account for 12% of the total area of ​​the cement pool, and set a drainage outlet at the bottom of the pool; the submerged plants are Elodea, yellow silk grass and water chestnut, which are planted at a planting area ratio of 1:5:6;

[0067] S2. Fish pond pretreatment: pour seawater into the fish pond to a height of 90 cm, and then sprinkle the Bacillus fermentation liquid accounting for 1.0% of the total volume of the seawater; the preparation method of the Bacillus fermentation liquid is to add wheat bran and sucrose to the seawater, stir evenly to obtain a mixture, and inoculate the mixture at a concentration of 4.0×10 9 CFU / mL of Paenibacillus polymyxa is aerobically fermented at a temperature of 32°C for 36 hours to obtain a fermentation product; the fermentation product is centrifuged and the supernatant is taken, which is the Bacillus fermentation liquid; wherein seawater, wheat bran and sucrose are mixed in a mass ratio of 70:7:1;

[0068] S3. Water quality management: adjust various parameters in the fish pond, water salinity 21‰, pH 6.0, dissolved oxygen 6mg / L, water temperature 23℃, nitrite content 0.070mg / L, water surface light intensity 2500Lx;

[0069] S4. Release fry: Release the hatched fry into the fish pond at a density of 1,100 fry / m 2 ;

[0070] S5. Bait management:

[0071] Early stage: on the 1st to 20th day after the fry enter the pond, feed the fish pond with artificial bait A, 4 times a day, at 09:00, 13:00, 18:00 and 21:00, and the feeding amount each time is 4% of the weight of the fry; the artificial bait A comprises the following raw materials by weight: 30 parts of earthworm powder, 12 parts of Cyperus rotundus, 8 parts of basil, 7 parts of Trifolium pratense, 3 parts of Smilax china and 60 parts of water;

[0072] The middle stage: on the 21st to 30th day after the fry enter the pond, feed the artificial feed B to the fish pond 3 times a day, at 09:00, 13:00 and 18:00, and the feeding amount each time is 8% of the weight of the fry; the artificial feed B comprises the following raw materials by weight: 20 parts of kelp powder, 15 parts of soybean meal, 10 parts of leech powder, 8 parts of catechu powder, 4 parts of beet red and 40 parts of water;

[0073] Later stage: 30 days after the fry enter the pond, feed the fish pond with artificial feed C twice a day, at 09:00 and 18:00, and the feeding amount each time is 14% of the weight of the fry; the artificial feed C comprises the following raw materials by weight: 18 parts of rapeseed cake, 18 parts of alfalfa, 11 parts of cinnamaldehyde, 10 parts of phloretin, 8 parts of lemon balm, 6 parts of valerian, 4 parts of red raspberry enzyme and 40 parts of water;

[0074] S6. Water quality replacement: After the fry enter the pond, the seawater is replaced every 8 days, and the amount of water replaced is 12% of the original fish pond water volume.

[0075] The preparation method of the artificial bait A comprises the following steps:

[0076] (1) adding β-mannanase to basil, red clover and smilax china and fermenting them for 5 days at a temperature of 60° C. to obtain a Chinese medicine fermentation product;

[0077] (2) Mixing and crushing the cyperus rotundus and the fermented Chinese medicine, adding earthworm powder and water and mixing evenly to prepare the raw material of artificial bait A;

[0078] (3) The raw material of artificial bait A is granulated by spray drying, using a spray gun type spray dryer, the temperature is 100° C., the spray gun pressure is 1.0 MPa, and the spray gun diameter is 0.8 mm, so as to obtain artificial bait A.

[0079] Comparative Example 1

[0080] The difference between this comparative example and Example 3 is that in step S1, no sea mud or sea sand is used for paving and submerged plants are planted in the fish pond, and the other components and steps remain unchanged.

[0081] A method for raising brown-spotted grouper fry comprises the following steps:

[0082] S1. Fish pond laying: Set up an indoor rectangular cement pool, clean the surroundings of the cement pool, disinfect it with quicklime before raising seedlings, and dry it for later use;

[0083] S2. Fish pond pretreatment: pour seawater into the fish pond to a height of 90 cm, and then sprinkle the Bacillus fermentation liquid accounting for 1.0% of the total volume of the seawater; the preparation method of the Bacillus fermentation liquid is to add wheat bran and sucrose to the seawater, stir evenly to obtain a mixture, and inoculate the mixture at a concentration of 4.0×109 CFU / mL of Paenibacillus polymyxa is aerobically fermented at a temperature of 32°C for 36 hours to obtain a fermentation product; the fermentation product is centrifuged and the supernatant is taken, which is the Bacillus fermentation liquid; wherein seawater, wheat bran and sucrose are mixed in a mass ratio of 70:7:1;

[0084] S3. Water quality management: adjust various parameters in the fish pond, water salinity 21‰, pH 6.0, dissolved oxygen 6mg / L, water temperature 23℃, nitrite content 0.070mg / L, water surface light intensity 2500Lx;

[0085] S4. Release fry: Release hatched fry into the fish pond at a density of 1,100 fry / m 2 ;

[0086] S5. Bait management:

[0087] Early stage: on the 1st to 20th day after the fry enter the pond, feed the fish pond with artificial bait A, 4 times a day, at 09:00, 13:00, 18:00 and 21:00, and the feeding amount each time is 4% of the weight of the fry; the artificial bait A comprises the following raw materials by weight: 30 parts of earthworm powder, 12 parts of Cyperus rotundus, 8 parts of basil, 7 parts of Trifolium pratense, 3 parts of Smilax china and 60 parts of water;

[0088] The middle stage: on the 21st to 30th day after the fry enter the pond, feed the artificial feed B to the fish pond 3 times a day, at 09:00, 13:00 and 18:00, and the feeding amount each time is 8% of the weight of the fry; the artificial feed B comprises the following raw materials by weight: 20 parts of kelp powder, 15 parts of soybean meal, 10 parts of leech powder, 8 parts of catechu powder, 4 parts of beet red and 40 parts of water;

[0089] Later stage: 30 days after the fry enter the pond, feed the fish pond with artificial feed C twice a day, at 09:00 and 18:00, and the feeding amount each time is 14% of the weight of the fry; the artificial feed C comprises the following raw materials by weight: 18 parts of rapeseed cake, 18 parts of alfalfa, 11 parts of cinnamaldehyde, 10 parts of phloretin, 8 parts of lemon balm, 6 parts of valerian, 4 parts of red raspberry enzyme and 40 parts of water;

[0090] S6. Water quality replacement: After the fry enter the pond, the seawater is replaced every 8 days, and the amount of water replaced is 12% of the original fish pond water volume.

[0091] The preparation method of the artificial bait A comprises the following steps:

[0092] (1) adding β-mannanase to basil, red clover and smilax china and fermenting them for 5 days at a temperature of 60° C. to obtain a Chinese medicine fermentation product;

[0093] (2) Mixing and crushing the cyperus rotundus and the fermented Chinese medicine, adding earthworm powder and water and mixing evenly to prepare the raw material of artificial bait A;

[0094] (3) The raw material of artificial bait A is granulated by spray drying, using a spray gun type spray dryer, the temperature is 100° C., the spray gun pressure is 1.0 MPa, and the spray gun diameter is 0.8 mm, so as to obtain artificial bait A.

[0095] Comparative Example 2

[0096] The difference between this comparative example and Example 3 is that in step S1, submerged plants selected are Poji Oocystis, Moss and Myriophyllum, and the other components and steps remain unchanged.

[0097] A method for raising brown-spotted grouper fry comprises the following steps:

[0098] S1. Fish pond laying: Set up an indoor rectangular cement pool, clean the surroundings of the cement pool, disinfect it with quicklime before raising seedlings, and dry it for later use; shovel in sea mud and sea sand in a weight ratio of 7:2 to cover the bottom of the cement pool, and the height after compaction is 10 cm, and then cultivate submerged plants that account for 12% of the total area of ​​the cement pool, and set a drainage outlet at the bottom of the pool; the submerged plants are Pogi oocysts, Moss and Foxtail algae, which are planted at a planting area ratio of 1:5:6;

[0099] S2. Fish pond pretreatment: pour seawater into the fish pond to a height of 90 cm, and then sprinkle the Bacillus fermentation liquid accounting for 1.0% of the total volume of the seawater; the preparation method of the Bacillus fermentation liquid is to add wheat bran and sucrose to the seawater, stir evenly to obtain a mixture, and inoculate the mixture at a concentration of 4.0×10 9 CFU / mL of Paenibacillus polymyxa is aerobically fermented at a temperature of 32°C for 36 hours to obtain a fermentation product; the fermentation product is centrifuged and the supernatant is taken, which is the Bacillus fermentation liquid; wherein seawater, wheat bran and sucrose are mixed in a mass ratio of 70:7:1;

[0100] S3. Water quality management: adjust various parameters in the fish pond, water salinity 21‰, pH 6.0, dissolved oxygen 6mg / L, water temperature 23℃, nitrite content 0.070mg / L, water surface light intensity 2500Lx;

[0101] S4. Release fry: Release hatched fry into the fish pond at a density of 1,100 fry / m 2 ;

[0102] S5. Bait management:

[0103] Early stage: on the 1st to 20th day after the fry enter the pond, feed the fish pond with artificial bait A, 4 times a day, at 09:00, 13:00, 18:00 and 21:00, and the feeding amount each time is 4% of the weight of the fry; the artificial bait A comprises the following raw materials by weight: 30 parts of earthworm powder, 12 parts of Cyperus rotundus, 8 parts of basil, 7 parts of Trifolium pratense, 3 parts of Smilax china and 60 parts of water;

[0104] The middle stage: on the 21st to 30th day after the fry enter the pond, feed the artificial feed B to the fish pond 3 times a day, at 09:00, 13:00 and 18:00, and the feeding amount each time is 8% of the weight of the fry; the artificial feed B comprises the following raw materials by weight: 20 parts of kelp powder, 15 parts of soybean meal, 10 parts of leech powder, 8 parts of catechu powder, 4 parts of beet red and 40 parts of water;

[0105] Later stage: 30 days after the fry enter the pond, feed the fish pond with artificial feed C twice a day, at 09:00 and 18:00, and the feeding amount each time is 14% of the weight of the fry; the artificial feed C comprises the following raw materials by weight: 18 parts of rapeseed cake, 18 parts of alfalfa, 11 parts of cinnamaldehyde, 10 parts of phloretin, 8 parts of lemon balm, 6 parts of valerian, 4 parts of red raspberry enzyme and 40 parts of water;

[0106] S6. Water quality replacement: After the fry enter the pond, the seawater is replaced every 8 days, and the amount of water replaced is 12% of the original fish pond water volume.

[0107] The preparation method of the artificial bait A comprises the following steps:

[0108] (1) adding β-mannanase to basil, red clover and smilax china and fermenting them for 5 days at a temperature of 60° C. to obtain a Chinese medicine fermentation product;

[0109] (2) Mixing and crushing the cyperus rotundus and the fermented Chinese medicine, adding earthworm powder and water and mixing evenly to prepare the raw material of artificial bait A;

[0110] (3) The raw material of artificial bait A is granulated by spray drying, using a spray gun type spray dryer, the temperature is 100° C., the spray gun pressure is 1.0 MPa, and the spray gun diameter is 0.8 mm, so as to obtain artificial bait A.

[0111] Comparative Example 3

[0112] The difference between this comparative example and Example 3 is that the Bacillus fermentation liquid is not sprayed in step S2, and the other components and steps remain unchanged.

[0113] A method for raising brown-spotted grouper fry comprises the following steps:

[0114] S1. Fish pond laying: Set up an indoor rectangular cement pool, clean the surroundings of the cement pool, disinfect it with quicklime before raising seedlings, and dry it for later use; shovel in sea mud and sea sand in a weight ratio of 7:2 to cover the bottom of the cement pool, and compact it to a height of 10 cm, then plant submerged plants that account for 12% of the total area of ​​the cement pool, and set a drainage outlet at the bottom of the pool; the submerged plants are Elodea, yellow silk grass and water chestnut, which are planted at a planting area ratio of 1:5:6;

[0115] S2. Fish pond pretreatment: Pour seawater into the fish pond to a height of 90 cm;

[0116] S3. Water quality management: adjust various parameters in the fish pond, water salinity 21‰, pH 6.0, dissolved oxygen 6mg / L, water temperature 23℃, nitrite content 0.070mg / L, water surface light intensity 2500Lx;

[0117] S4. Release fry: Release hatched fry into the fish pond at a density of 1,100 fry / m 2 ;

[0118] S5. Bait management:

[0119] Early stage: on the 1st to 20th day after the fry enter the pond, feed the fish pond with artificial bait A, 4 times a day, at 09:00, 13:00, 18:00 and 21:00, and the feeding amount each time is 4% of the weight of the fry; the artificial bait A comprises the following raw materials by weight: 30 parts of earthworm powder, 12 parts of Cyperus rotundus, 8 parts of basil, 7 parts of Trifolium pratense, 3 parts of Smilax china and 60 parts of water;

[0120] The middle stage: on the 21st to 30th day after the fry enter the pond, feed the artificial feed B to the fish pond 3 times a day, at 09:00, 13:00 and 18:00, and the feeding amount each time is 8% of the weight of the fry; the artificial feed B comprises the following raw materials by weight: 20 parts of kelp powder, 15 parts of soybean meal, 10 parts of leech powder, 8 parts of catechu powder, 4 parts of beet red and 40 parts of water;

[0121] Later stage: 30 days after the fry enter the pond, feed the fish pond with artificial feed C twice a day, at 09:00 and 18:00, and the feeding amount each time is 14% of the weight of the fry; the artificial feed C comprises the following raw materials by weight: 18 parts of rapeseed cake, 18 parts of alfalfa, 11 parts of cinnamaldehyde, 10 parts of phloretin, 8 parts of lemon balm, 6 parts of valerian, 4 parts of red raspberry enzyme and 40 parts of water;

[0122] S6. Water quality replacement: After the fry enter the pond, the seawater is replaced every 8 days, and the amount of water replaced is 12% of the original fish pond water volume.

[0123] The preparation method of the artificial bait A comprises the following steps:

[0124] (1) adding β-mannanase to basil, red clover and smilax china and fermenting them for 5 days at a temperature of 60° C. to obtain a Chinese medicine fermentation product;

[0125] (2) Mixing and crushing the cyperus rotundus and the fermented Chinese medicine, adding earthworm powder and water and mixing evenly to prepare the raw material of artificial bait A;

[0126] (3) The raw material of artificial bait A is granulated by spray drying, using a spray gun type spray dryer, the temperature is 100° C., the spray gun pressure is 1.0 MPa, and the spray gun diameter is 0.8 mm, so as to obtain artificial bait A.

[0127] Comparative Example 4

[0128] The difference between this comparative example and Example 3 is that in step S2, the polymyxa bacillus is replaced with EM bacterial agent, and the other components and steps remain unchanged.

[0129] A method for raising brown-spotted grouper fry comprises the following steps:

[0130] S1. Fish pond laying: Set up an indoor rectangular cement pool, clean the surroundings of the cement pool, disinfect it with quicklime before raising seedlings, and dry it for later use; shovel in sea mud and sea sand in a weight ratio of 7:2 to cover the bottom of the cement pool, and compact it to a height of 10 cm, then plant submerged plants that account for 12% of the total area of ​​the cement pool, and set a drainage outlet at the bottom of the pool; the submerged plants are Elodea, yellow silk grass and water chestnut, which are planted at a planting area ratio of 1:5:6;

[0131] S2. Fish pond pretreatment: pour seawater into the fish pond to a height of 90 cm, and then sprinkle EM bacterial fermentation liquid accounting for 1.0% of the total volume of seawater; the preparation method of the bacterial fermentation liquid is to add wheat bran and sucrose to the seawater, stir evenly to obtain a mixture, and inoculate at a concentration of 4.0×10 9 CFU / mL of EM bacterial agent, aerobically fermented at a temperature of 32°C for 36 hours to obtain a fermentation product; the fermentation product was centrifuged and the supernatant was taken, which was the EM bacterial fermentation liquid; wherein seawater, wheat bran and sucrose were mixed in a mass ratio of 70:7:1;

[0132] S3. Water quality management: adjust various parameters in the fish pond, water salinity 21‰, pH 6.0, dissolved oxygen 6mg / L, water temperature 23℃, nitrite content 0.070mg / L, water surface light intensity 2500Lx;

[0133] S4. Release fry: Release hatched fry into the fish pond at a density of 1,100 fry / m 2 ;

[0134] S5. Bait management:

[0135] Early stage: on the 1st to 20th day after the fry enter the pond, feed the fish pond with artificial bait A, 4 times a day, at 09:00, 13:00, 18:00 and 21:00, and the feeding amount each time is 4% of the weight of the fry; the artificial bait A comprises the following raw materials by weight: 30 parts of earthworm powder, 12 parts of Cyperus rotundus, 8 parts of basil, 7 parts of Trifolium pratense, 3 parts of Smilax china and 60 parts of water;

[0136] The middle stage: on the 21st to 30th day after the fry enter the pond, feed the artificial feed B to the fish pond 3 times a day, at 09:00, 13:00 and 18:00, and the feeding amount each time is 8% of the weight of the fry; the artificial feed B comprises the following raw materials by weight: 20 parts of kelp powder, 15 parts of soybean meal, 10 parts of leech powder, 8 parts of catechu powder, 4 parts of beet red and 40 parts of water;

[0137] Later stage: 30 days after the fry enter the pond, feed the fish pond with artificial feed C twice a day, at 09:00 and 18:00, and the feeding amount each time is 14% of the weight of the fry; the artificial feed C comprises the following raw materials by weight: 18 parts of rapeseed cake, 18 parts of alfalfa, 11 parts of cinnamaldehyde, 10 parts of phloretin, 8 parts of lemon balm, 6 parts of valerian, 4 parts of red raspberry enzyme and 40 parts of water;

[0138] S6. Water quality replacement: After the fry enter the pond, the seawater is replaced every 8 days, and the amount of water replaced is 12% of the original fish pond water volume.

[0139] The preparation method of the artificial bait A comprises the following steps:

[0140] (1) adding β-mannanase to basil, red clover and smilax china and fermenting them for 5 days at a temperature of 60° C. to obtain a Chinese medicine fermentation product;

[0141] (2) Mixing and crushing the cyperus rotundus and the fermented Chinese medicine, adding earthworm powder and water and mixing evenly to prepare the raw material of artificial bait A;

[0142] (3) The raw material of artificial bait A is granulated by spray drying, using a spray gun type spray dryer, the temperature is 100° C., the spray gun pressure is 1.0 MPa, and the spray gun diameter is 0.8 mm, so as to obtain artificial bait A.

[0143] Comparative Example 5

[0144] The difference between this comparative example and Example 3 is that artificial feed A is fed in the middle and late stages, and the other ingredients and steps remain unchanged.

[0145] A method for raising brown-spotted grouper fry comprises the following steps:

[0146] S1. Fish pond laying: Set up an indoor rectangular cement pool, clean the surroundings of the cement pool, disinfect it with quicklime before raising seedlings, and dry it for later use; shovel in sea mud and sea sand in a weight ratio of 7:2 to cover the bottom of the cement pool, and compact it to a height of 10 cm, then plant submerged plants that account for 12% of the total area of ​​the cement pool, and set a drainage outlet at the bottom of the pool; the submerged plants are Elodea, yellow silk grass and water chestnut, which are planted at a planting area ratio of 1:5:6;

[0147] S2. Fish pond pretreatment: pour seawater into the fish pond to a height of 90 cm, and then sprinkle the Bacillus fermentation liquid accounting for 1.0% of the total volume of the seawater; the preparation method of the Bacillus fermentation liquid is to add wheat bran and sucrose to the seawater, stir evenly to obtain a mixture, and inoculate the mixture at a concentration of 4.0×10 9 CFU / mL of Paenibacillus polymyxa is aerobically fermented at a temperature of 32°C for 36 hours to obtain a fermentation product; the fermentation product is centrifuged and the supernatant is taken, which is the Bacillus fermentation liquid; wherein seawater, wheat bran and sucrose are mixed in a mass ratio of 70:7:1;

[0148] S3. Water quality management: adjust various parameters in the fish pond, water salinity 21‰, pH 6.0, dissolved oxygen 6mg / L, water temperature 23℃, nitrite content 0.070mg / L, water surface light intensity 2500Lx;

[0149] S4. Release fry: Release hatched fry into the fish pond at a density of 1,100 fry / m 2 ;

[0150] S5. Bait management:

[0151] Early stage: on the 1st to 20th day after the fry enter the pond, feed the fish pond with artificial bait A, 4 times a day, at 09:00, 13:00, 18:00 and 21:00, and the feeding amount each time is 4% of the weight of the fry; the artificial bait A comprises the following raw materials by weight: 30 parts of earthworm powder, 12 parts of Cyperus rotundus, 8 parts of basil, 7 parts of Trifolium pratense, 3 parts of Smilax china and 60 parts of water;

[0152] Mid-term stage: On the 21st to 30th day after the fry enter the pond, feed the artificial feed A to the fish pond 3 times a day at 09:00, 13:00 and 18:00, and the feeding amount each time is 8% of the fry weight;

[0153] Late stage: 30 days after the fry were placed in the pond, artificial feed A was fed into the pond twice a day at 09:00 and 18:00, and the feeding amount each time was 14% of the fry weight;

[0154] S6. Water quality replacement: After the fry enter the pond, the seawater is replaced every 8 days, and the amount of water replaced is 12% of the original fish pond water volume.

[0155] The preparation method of the artificial bait A comprises the following steps:

[0156] (1) adding β-mannanase to basil, red clover and smilax china and fermenting them for 5 days at a temperature of 60° C. to obtain a Chinese medicine fermentation product;

[0157] (2) Mixing and crushing the cyperus rotundus and the fermented Chinese medicine, adding earthworm powder and water and mixing evenly to prepare the raw material of artificial bait A;

[0158] (3) The raw material of artificial bait A is granulated by spray drying, using a spray gun type spray dryer, the temperature is 100° C., the spray gun pressure is 1.0 MPa, and the spray gun diameter is 0.8 mm, so as to obtain artificial bait A.

[0159] Comparative Example 6

[0160] The difference between this comparative example and Example 3 is that artificial feed B is fed in the early and late stages, and the other ingredients and steps remain unchanged.

[0161] A method for raising brown-spotted grouper fry comprises the following steps:

[0162] S1. Fish pond laying: Set up an indoor rectangular cement pool, clean the surroundings of the cement pool, disinfect it with quicklime before raising seedlings, and dry it for later use; shovel in sea mud and sea sand in a weight ratio of 7:2 to cover the bottom of the cement pool, and compact it to a height of 10 cm, then plant submerged plants that account for 12% of the total area of ​​the cement pool, and set a drainage outlet at the bottom of the pool; the submerged plants are Elodea, yellow silk grass and water chestnut, which are planted at a planting area ratio of 1:5:6;

[0163] S2. Fish pond pretreatment: pour seawater into the fish pond to a height of 90 cm, and then sprinkle the Bacillus fermentation liquid accounting for 1.0% of the total volume of the seawater; the preparation method of the Bacillus fermentation liquid is to add wheat bran and sucrose to the seawater, stir evenly to obtain a mixture, and inoculate the mixture at a concentration of 4.0×10 9 CFU / mL of Paenibacillus polymyxa is aerobically fermented at a temperature of 32°C for 36 hours to obtain a fermentation product; the fermentation product is centrifuged and the supernatant is taken, which is the Bacillus fermentation liquid; wherein seawater, wheat bran and sucrose are mixed in a mass ratio of 70:7:1;

[0164] S3. Water quality management: adjust various parameters in the fish pond, water salinity 21‰, pH 6.0, dissolved oxygen 6mg / L, water temperature 23℃, nitrite content 0.070mg / L, water surface light intensity 2500Lx;

[0165] S4. Release fry: Release hatched fry into the fish pond at a density of 1,100 fry / m 2 ;

[0166] S5. Bait management:

[0167] Early stage: on the 1st to 20th day after the fry enter the pond, feed the fish pond with artificial feed B, 4 times a day, at 09:00, 13:00, 18:00 and 21:00, and the feeding amount each time is 4% of the weight of the fry; the artificial feed B comprises the following raw materials by weight: 20 parts of kelp powder, 15 parts of soybean meal, 10 parts of leech powder, 8 parts of catechu powder, 4 parts of beet red and 40 parts of water;

[0168] Mid-term stage: On the 21st to 30th day after the fry enter the pond, feed the artificial feed B 3 times a day at 09:00, 13:00 and 18:00, and the feeding amount each time is 8% of the fry weight;

[0169] Late stage: 30 days after the fry were placed in the pond, artificial feed B was fed into the pond twice a day at 09:00 and 18:00, and the feeding amount each time was 14% of the fry weight;

[0170] S6. Water quality replacement: After the fry enter the pond, the seawater is replaced every 8 days, and the amount of water replaced is 12% of the original fish pond water volume.

[0171] Comparative Example 7

[0172] The difference between this comparative example and Example 3 is that artificial feed C is fed in the early and middle stages, and the other ingredients and steps remain unchanged.

[0173] A method for raising brown-spotted grouper fry comprises the following steps:

[0174] S1. Fish pond laying: Set up an indoor rectangular cement pool, clean the surroundings of the cement pool, disinfect it with quicklime before raising seedlings, and dry it for later use; shovel in sea mud and sea sand in a weight ratio of 7:2 to cover the bottom of the cement pool, and compact it to a height of 10 cm, then plant submerged plants that account for 12% of the total area of ​​the cement pool, and set a drainage outlet at the bottom of the pool; the submerged plants are Elodea, yellow silk grass and water chestnut, which are planted at a planting area ratio of 1:5:6;

[0175] S2. Fish pond pretreatment: pour seawater into the fish pond to a height of 90 cm, and then sprinkle the Bacillus fermentation liquid accounting for 1.0% of the total volume of the seawater; the preparation method of the Bacillus fermentation liquid is to add wheat bran and sucrose to the seawater, stir evenly to obtain a mixture, and inoculate the mixture at a concentration of 4.0×10 9CFU / mL of Paenibacillus polymyxa is aerobically fermented at a temperature of 32°C for 36 hours to obtain a fermentation product; the fermentation product is centrifuged and the supernatant is taken, which is the Bacillus fermentation liquid; wherein seawater, wheat bran and sucrose are mixed in a mass ratio of 70:7:1;

[0176] S3. Water quality management: adjust various parameters in the fish pond, water salinity 21‰, pH 6.0, dissolved oxygen 6mg / L, water temperature 23℃, nitrite content 0.070mg / L, water surface light intensity 2500Lx;

[0177] S4. Release fry: Release the hatched fry into the fish pond at a density of 1,100 fry / m 2 ;

[0178] S5. Bait management:

[0179] Early stage: on the 1st to 20th day after the fry enter the pond, artificial feed C is fed into the fish pond 4 times a day, at 09:00, 13:00, 18:00 and 21:00, and the feeding amount each time is 4% of the weight of the fry; the artificial feed C comprises the following raw materials by weight: 18 parts of rapeseed cake, 18 parts of alfalfa, 11 parts of cinnamaldehyde, 10 parts of phloretin, 8 parts of lemon balm, 6 parts of valerian, 4 parts of red raspberry enzyme and 40 parts of water;

[0180] Mid-term stage: On the 21st to 30th day after the fry enter the pond, feed the artificial feed C to the fish pond 3 times a day at 09:00, 13:00 and 18:00, and the feeding amount each time is 8% of the fry weight;

[0181] Late stage: 30 days after the fry were placed in the pond, artificial feed C was fed into the pond twice a day at 09:00 and 18:00, and the feeding amount each time was 14% of the fry weight;

[0182] S6. Water quality replacement: After the fry enter the pond, the seawater is replaced every 8 days, and the amount of water replaced is 12% of the original fish pond water volume.

[0183] Comparative Example 8

[0184] The difference between this comparative example and Example 3 is that the preparation method of artificial bait A is changed, and β-mannanase fermentation is not used, and the other ingredients and steps remain unchanged.

[0185] A method for raising brown-spotted grouper fry comprises the following steps:

[0186] S1. Fish pond laying: Set up an indoor rectangular cement pool, clean the surroundings of the cement pool, disinfect it with quicklime before raising seedlings, and dry it for later use; shovel in sea mud and sea sand in a weight ratio of 7:2 to cover the bottom of the cement pool, and compact it to a height of 10 cm, then plant submerged plants that account for 12% of the total area of ​​the cement pool, and set a drainage outlet at the bottom of the pool; the submerged plants are Elodea, yellow silk grass and water chestnut, which are planted at a planting area ratio of 1:5:6;

[0187] S2. Fish pond pretreatment: pour seawater into the fish pond to a height of 90 cm, and then sprinkle the Bacillus fermentation liquid accounting for 1.0% of the total volume of the seawater; the preparation method of the Bacillus fermentation liquid is to add wheat bran and sucrose to the seawater, stir evenly to obtain a mixture, and inoculate the mixture at a concentration of 4.0×10 9 CFU / mL of Paenibacillus polymyxa is aerobically fermented at a temperature of 32°C for 36 hours to obtain a fermentation product; the fermentation product is centrifuged and the supernatant is taken, which is the Bacillus fermentation liquid; wherein seawater, wheat bran and sucrose are mixed in a mass ratio of 70:7:1;

[0188] S3. Water quality management: adjust various parameters in the fish pond, water salinity 21‰, pH 6.0, dissolved oxygen 6mg / L, water temperature 23℃, nitrite content 0.070mg / L, water surface light intensity 2500Lx;

[0189] S4. Release fry: Release hatched fry into the fish pond at a density of 1,100 fry / m 2 ;

[0190] S5. Bait management:

[0191] Early stage: on the 1st to 20th day after the fry enter the pond, feed the fish pond with artificial bait A, 4 times a day, at 09:00, 13:00, 18:00 and 21:00, and the feeding amount each time is 4% of the weight of the fry; the artificial bait A comprises the following raw materials by weight: 30 parts of earthworm powder, 12 parts of Cyperus rotundus, 8 parts of basil, 7 parts of Trifolium pratense, 3 parts of Smilax china and 60 parts of water;

[0192] The middle stage: on the 21st to 30th day after the fry enter the pond, feed the artificial feed B to the fish pond 3 times a day, at 09:00, 13:00 and 18:00, and the feeding amount each time is 8% of the weight of the fry; the artificial feed B comprises the following raw materials by weight: 20 parts of kelp powder, 15 parts of soybean meal, 10 parts of leech powder, 8 parts of catechu powder, 4 parts of beet red and 40 parts of water;

[0193] Later stage: 30 days after the fry enter the pond, feed the fish pond with artificial feed C twice a day, at 09:00 and 18:00, and the feeding amount each time is 14% of the weight of the fry; the artificial feed C comprises the following raw materials by weight: 18 parts of rapeseed cake, 18 parts of alfalfa, 11 parts of cinnamaldehyde, 10 parts of phloretin, 8 parts of lemon balm, 6 parts of valerian, 4 parts of red raspberry enzyme and 40 parts of water;

[0194] S6. Water quality replacement: After the fry enter the pond, the seawater is replaced every 8 days, and the amount of water replaced is 12% of the original fish pond water volume.

[0195] The preparation method of the artificial bait A comprises the following steps:

[0196] (1) fermenting basil, red clover and smilax china for 5 days at a temperature of 60° C. to obtain a Chinese medicine fermentation product;

[0197] (2) Mixing and crushing the cyperus rotundus and the fermented Chinese medicine, adding earthworm powder and water and mixing evenly to prepare the raw material of artificial bait A;

[0198] (3) The raw material of artificial bait A is granulated by spray drying, using a spray gun type spray dryer, the temperature is 100° C., the spray gun pressure is 1.0 MPa, and the spray gun diameter is 0.8 mm, so as to obtain artificial bait A.

[0199] Comparative Example 9

[0200] The difference between this comparative example and Example 3 is that artificial bait C does not include red raspberry enzyme, and the other ingredients and steps remain unchanged.

[0201] A method for raising brown-spotted grouper fry comprises the following steps:

[0202] S1. Fish pond laying: Set up an indoor rectangular cement pool, clean the surroundings of the cement pool, disinfect it with quicklime before raising seedlings, and dry it for later use; shovel in sea mud and sea sand in a weight ratio of 7:2 to cover the bottom of the cement pool, and compact it to a height of 10 cm, then plant submerged plants that account for 12% of the total area of ​​the cement pool, and set a drainage outlet at the bottom of the pool; the submerged plants are Elodea, yellow silk grass and water chestnut, which are planted at a planting area ratio of 1:5:6;

[0203] S2. Fish pond pretreatment: pour seawater into the fish pond to a height of 90 cm, and then sprinkle the Bacillus fermentation liquid accounting for 1.0% of the total volume of the seawater; the preparation method of the Bacillus fermentation liquid is to add wheat bran and sucrose to the seawater, stir evenly to obtain a mixture, and inoculate the mixture at a concentration of 4.0×10 9CFU / mL of Paenibacillus polymyxa is aerobically fermented at a temperature of 32°C for 36 hours to obtain a fermentation product; the fermentation product is centrifuged and the supernatant is taken, which is the Bacillus fermentation liquid; wherein seawater, wheat bran and sucrose are mixed in a mass ratio of 70:7:1;

[0204] S3. Water quality management: adjust various parameters in the fish pond, water salinity 21‰, pH 6.0, dissolved oxygen 6mg / L, water temperature 23℃, nitrite content 0.070mg / L, water surface light intensity 2500Lx;

[0205] S4. Release fry: Release hatched fry into the fish pond at a density of 1,100 fry / m 2 ;

[0206] S5. Bait management:

[0207] Early stage: on the 1st to 20th day after the fry enter the pond, feed the fish pond with artificial bait A, 4 times a day, at 09:00, 13:00, 18:00 and 21:00, and the feeding amount each time is 4% of the weight of the fry; the artificial bait A comprises the following raw materials by weight: 30 parts of earthworm powder, 12 parts of Cyperus rotundus, 8 parts of basil, 7 parts of Trifolium pratense, 3 parts of Smilax china and 60 parts of water;

[0208] The middle stage: on the 21st to 30th day after the fry enter the pond, feed the artificial feed B to the fish pond 3 times a day, at 09:00, 13:00 and 18:00, and the feeding amount each time is 8% of the weight of the fry; the artificial feed B comprises the following raw materials by weight: 20 parts of kelp powder, 15 parts of soybean meal, 10 parts of leech powder, 8 parts of catechu powder, 4 parts of beet red and 40 parts of water;

[0209] Later stage: 30 days after the fry enter the pond, feeding artificial feed C to the fish pond, feeding twice a day, feeding time points are 09:00 and 18:00, and each feeding amount is 14% of the weight of the fry; the artificial feed C comprises the following raw materials by weight: 18 parts of rapeseed cake, 18 parts of alfalfa, 11 parts of cinnamaldehyde, 10 parts of phloretin, 8 parts of lemon balm, 6 parts of valerian and 40 parts of water;

[0210] S6. Water quality replacement: After the fry enter the pond, the seawater is replaced every 8 days, and the amount of water replaced is 12% of the original fish pond water volume.

[0211] The preparation method of the artificial bait A comprises the following steps:

[0212] (1) adding β-mannanase to basil, red clover and smilax china and fermenting them for 5 days at a temperature of 60° C. to obtain a Chinese medicine fermentation product;

[0213] (2) Mixing and crushing the cyperus rotundus and the fermented Chinese medicine, adding earthworm powder and water and mixing evenly to prepare the raw material of artificial bait A;

[0214] (3) The raw material of artificial bait A is granulated by spray drying, using a spray gun type spray dryer, the temperature is 100° C., the spray gun pressure is 1.0 MPa, and the spray gun diameter is 0.8 mm, so as to obtain artificial bait A.

[0215] Comparative Example 10

[0216] The difference between this comparative example and Example 3 is that artificial bait A, artificial bait B and artificial bait C are all replaced with commercially available feed (ruby grouper feed), and the other ingredients and steps remain unchanged.

[0217] A method for raising brown-spotted grouper fry comprises the following steps:

[0218] S1. Fish pond laying: Set up an indoor rectangular cement pool, clean the surroundings of the cement pool, disinfect it with quicklime before raising seedlings, and dry it for later use; shovel in sea mud and sea sand in a weight ratio of 7:2 to cover the bottom of the cement pool, and compact it to a height of 10 cm, then plant submerged plants that account for 12% of the total area of ​​the cement pool, and set a drainage outlet at the bottom of the pool; the submerged plants are Elodea, yellow silk grass and water chestnut, which are planted at a planting area ratio of 1:5:6;

[0219] S2. Fish pond pretreatment: pour seawater into the fish pond to a height of 90 cm, and then sprinkle the Bacillus fermentation liquid accounting for 1.0% of the total volume of the seawater; the preparation method of the Bacillus fermentation liquid is to add wheat bran and sucrose to the seawater, stir evenly to obtain a mixture, and inoculate the mixture at a concentration of 4.0×10 9 CFU / mL of Paenibacillus polymyxa is aerobically fermented at a temperature of 32°C for 36 hours to obtain a fermentation product; the fermentation product is centrifuged and the supernatant is taken, which is the Bacillus fermentation liquid; wherein seawater, wheat bran and sucrose are mixed in a mass ratio of 70:7:1;

[0220] S3. Water quality management: adjust various parameters in the fish pond, water salinity 21‰, pH 6.0, dissolved oxygen 6mg / L, water temperature 23℃, nitrite content 0.070mg / L, water surface light intensity 2500Lx;

[0221] S4. Release fry: Release hatched fry into the fish pond at a density of 1,100 fry / m 2 ;

[0222] S5. Bait management:

[0223] Early stage: From the 1st to the 20th day after the fry enter the pond, feed the fish pond with commercial feed 4 times a day at 09:00, 13:00, 18:00 and 21:00, and the feeding amount each time is 4% of the weight of the fry;

[0224] Mid-term stage: On the 21st to 30th day after the fry enter the pond, feed the fish pond with commercial feed 3 times a day at 09:00, 13:00 and 18:00, and the feeding amount each time is 8% of the fry weight;

[0225] Late stage: 30 days after the fry are placed in the pond, commercial feed is fed into the fish pond twice a day at 09:00 and 18:00, and the feeding amount each time is 14% of the fry weight;

[0226] S6. Water quality replacement: After the fry enter the pond, the seawater is replaced every 8 days, and the amount of water replaced is 12% of the original fish pond water volume.

[0227] Experimental example

[0228] (1) Survival rate test

[0229] Brown-spotted grouper was raised for 45 days according to the raising methods of Examples 1 to 3 and Comparative Examples 1 to 9, and the survival rate of the young fish was counted. The results are shown in Table 1:

[0230] Table 1 Survival rate of juvenile fish

[0231]

[0232]

[0233] As can be seen from Table 1, Examples 1 to 3 can improve the survival rate of young fish through the reasonable construction of the ecological environment of the fish pond, appropriate fish pond pretreatment, water quality management and bait management measures, especially Example 3 has the best effect. Compared with Example 1, it can be seen that creating a living environment for wild brown-spotted grouper is conducive to improving its survival rate. The sea mud is rich in organic matter and minerals, which provides a rich food source for marine life; compared with Example 2, it can be seen that the selection of submerged plants such as Elodea, yellow silk grass and water chestnut is more conducive to the stable operation of the aquatic ecosystem; compared with Example 3 and Comparative Examples 3 and 4, it can be seen that it is very important to spray the fish pond with Bacillus fermentation liquid. Bacillus is a food source for zooplankton such as protozoa, rotifers and copepods in the water body. Spraying the beneficial bacteria fermentation liquid into the fish pond It can lead to an increase in zooplankton in the water body, enrich the nutritional bait of brown-spotted grouper, enhance the physique of brown-spotted grouper, thereby enhancing its disease resistance, improving the survival rate, and having a certain selectivity for the sprayed bacterial agent; Example 3 and Comparative Examples 5 to 10 show that the choice of bait during the seedling raising process greatly affects the survival rate of young fish, and the young fish ingest feed to obtain the required nutrients therefrom. If the feed is not nutritious, it will cause the death of the young fish, and the commercially available feed cannot meet the growth nutritional needs of brown-spotted grouper. The artificial bait of the present invention is rich in a variety of nutrients and can reduce the mortality rate of young fish. The present invention uses the mutual coordination of Chinese medicinal materials, β-mannanase and red raspberry enzyme in the artificial bait, β-mannanase can increase the utilization rate of bait nutrition, improve bait conversion efficiency and production performance, and red raspberry enzyme can regulate the gastrointestinal flora ecosystem of young fish, improve the morphological structure of intestinal mucosa, and improve the absorption of nutrients, thereby reducing the mortality rate of young fish.

[0234] (2) Fish index test

[0235] After counting the survival rate of fry, 20 fry were randomly picked for each fry rearing method to test fish indicators. The total protein test was tested by the fish total protein (TP) ELISA kit purchased from Shanghai Qincheng Biotechnology Co., Ltd.; the cholesterol test was tested by the (TC) ELISA kit purchased from Shenzhen Zike Biotechnology Co., Ltd.; the muscle content was tested according to GB / T 18654.5-2008 "Farmed fish germplasm inspection (Part 5): Food habit analysis";

[0236] The test results are shown in Table 2.

[0237] Table 2 Fish index test

[0238]

[0239]

[0240] As can be seen from Table 2, the fry cultured by the seedling raising method of the present invention has high total protein, cholesterol and muscle content, especially the fry cultured in Example 3. Selecting suitable artificial bait, feeding the bait in different time periods, constructing a suitable aquatic ecological environment and selecting and spraying suitable microorganisms can improve fish test indicators and improve the economic value of brown spot grouper. This seedling raising method is conducive to large-scale promotion.

[0241] The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A method for raising brown-spotted grouper, characterized in that: The following steps are involved: S1. Fish pond laying: Set up an indoor cement pool, clean the surroundings of the cement pool, disinfect it before raising seedlings, and dry it for later use; shovel in sea mud and sea sand to cover the bottom of the cement pool, compact it to a height of 8 to 12 cm, and then plant submerged plants; S2, fish pond pretreatment: pour seawater into the fish pond, and then splash the Bacillus fermentation liquid accounting for 0.5-1.5% of the total volume of seawater; S3. Water quality management: Control water salinity at 20-25‰, pH at 5.5-6.5, dissolved oxygen at 4-8mg / L, water temperature at 22-25℃, nitrite content at 0.050-0.080mg / L, and light intensity on the water surface at 2000-2800Lx; S4. Release fry: release the hatched fry into the fish pond; S5. Bait management: Early stage: on the 1st to 20th day after the fry enter the pond, feed the fish pond with artificial bait A, and the feeding amount each time is 3-5% of the weight of the fry; the artificial bait A comprises the following raw materials by weight: 20-35 parts of earthworm powder, 10-15 parts of Cyperus oleifera, 7-10 parts of basil, 5-8 parts of Trifolium pratense, 1-4 parts of Smilax china and 50-70 parts of water; The middle stage: on the 21st to 30th day after the fry enter the pond, feed the fish pond with artificial feed B, and the feeding amount each time is 6 to 10% of the weight of the fry; the artificial feed B comprises the following raw materials by weight: 15 to 25 parts of kelp powder, 10 to 20 parts of soybean meal, 8 to 15 parts of leech powder, 5 to 10 parts of catechu powder, 2 to 6 parts of beet red and 30 to 45 parts of water; The later stage: 30 days after the fry enter the pond, feed the fish pond with artificial bait C, and the feeding amount each time is 12-15% of the weight of the fry; the artificial bait C comprises the following raw materials by weight: 12-30 parts of rapeseed cake, 12-22 parts of alfalfa, 10-15 parts of cinnamaldehyde, 8-15 parts of phloretin, 5-9 parts of lemon balm, 3-7 parts of valerian, 1-5 parts of red raspberry enzyme and 35-45 parts of water; S6. Water quality replacement: After the fry enter the pond, the seawater should be replaced every 7 to 10 days, and the amount of water replaced is 10 to 15% of the original pond water volume.

2. A method for raising seedlings of brown-spotted grouper according to claim 1, characterized in that: The weight ratio of sea mud to sea sand in step S1 is (5-9):(1-3).

3. A method for raising seedlings of brown-spotted grouper according to claim 1, characterized in that: In step S1, the submerged plants are Elodea, Psoralea corylifolia and Potamogeton crispus, which are planted at a planting area ratio of 1:(3-7):(1-8), accounting for 10-15% of the total area of ​​the cement pool.

4. A method for raising seedlings of brown-spotted grouper according to claim 1, characterized in that: The preparation method of the Bacillus fermentation liquid in step S2 is to add wheat bran and sucrose to seawater, stir evenly to obtain a mixture, and inoculate at a concentration of 3.0×10 7 ~5.0×10 9 The polymyxa bacillus with a CFU / mL is aerobically fermented at a temperature of 30-35°C for 48-72 hours to obtain a fermentation product; the fermentation product is centrifuged and the supernatant is taken, which is the bacillus fermentation broth.

5. A method for raising seedlings of brown-spotted grouper according to claim 4, characterized in that: Seawater, wheat bran and sucrose are mixed in a mass ratio of (60-80):(6-9):

1.

6. A method for raising seedlings of brown-spotted grouper according to claim 1, characterized in that: In step S4, the density of fry is 1000 to 1500 fish / m 2 .

7. A method for raising seedlings of brown-spotted grouper according to claim 1, characterized in that: The preparation method of artificial bait A in step S5 comprises the following steps: (1) adding β-mannanase to basil, red clover and smilax china and fermenting them for 3 to 7 days at a temperature of 55 to 65° C. to obtain a Chinese medicine fermentation product; (2) Mixing and crushing the cyperus rotundus and the fermented Chinese medicine, adding earthworm powder and water and mixing evenly to prepare the raw material of artificial bait A; (3) The artificial bait A raw material is granulated by a spray drying method to obtain artificial bait A.

8. A method for raising seedlings of brown-spotted grouper according to claim 1, characterized in that: The artificial bait A in step S5 comprises the following raw materials in parts by weight: 30 parts of earthworm meal, 12 parts of Cyperus oleifera, 8 parts of basil, 7 parts of Trifolium pratense, 3 parts of Smilax china and 60 parts of water.

9. A method for raising seedlings of brown-spotted grouper according to claim 1, characterized in that: The artificial bait B in step S5 comprises the following raw materials in parts by weight: 20 parts of kelp powder, 15 parts of soybean meal, 10 parts of leech powder, 8 parts of catechu powder, 4 parts of beetroot red and 40 parts of water.

10. The method for raising seedlings of brown-spotted grouper according to claim 1, characterized in that: The artificial bait C in step S5 comprises the following raw materials in parts by weight: 18 parts of rapeseed cake, 18 parts of alfalfa, 11 parts of cinnamaldehyde, 10 parts of phloretin, 8 parts of lemon balm, 6 parts of valerian, 4 parts of red raspberry enzyme and 40 parts of water.

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