A Camellia oil anti-wrinkle, anti-aging and antioxidant cream and its preparation method

Abstract

The present invention provides a camellia oil anti-wrinkle, anti-aging, antioxidant cream and a preparation method thereof, belonging to the technical field of cosmetics. The preparation method comprises the following steps: adding glycerol, 1,3-butanediol, sorbitol, sodium hyaluronate, ethylhexylglycerin, methylparaben into water according to the formula, heating and stirring for 10-20 min, then successively adding camellia oil, plant fermentation active substances, squalane, L-arginine, tocopherol, PEG-7 glyceryl cocoate, cetearyl alcohol, glyceryl behenate and continuing to stir for 20-40 min, then homogenizing for 4-6 min, sterilizing, and discharging to obtain the product; wherein, the plant fermentation active substances are obtained by crushing, air explosion and fermentation treatment of Erigeron breviscapus and Plantago asiatica, and then through heating extraction and supercritical extraction; the fermentation bacteria are Bacillus subtilis and / or Lactiplantibacillus plantarum. The cream of the present invention has stable storage performance, is mild and moisturizing, can timely supplement moisture and nutrition to the skin, and has excellent antioxidant, anti-aging and anti-wrinkle effects.

Description

Technical Field

[0001] The present invention belongs to the technical field of cosmetics, and particularly relates to a camellia oil anti-wrinkle, anti-aging, antioxidant cream and a preparation method thereof. Background Art

[0002] Camellia oil, derived from the seeds of Camellia japonica or Camellia oleifera, is a precious natural vegetable oil, famous for its rich unsaturated fatty acids (such as oleic acid and linoleic acid), camellia glycosides, vitamins (such as vitamin E), and tea saponol. Among them, unsaturated fatty acids can penetrate the skin, provide deep moisturization, help maintain the skin's water-oil balance, and keep the skin elastic and shiny. Antioxidant components such as vitamins and tea saponol can resist the damage of free radicals to skin cells, reduce inflammatory reactions, and promote skin health. Active substances such as camellia glycosides help promote the regeneration of skin cells and accelerate wound healing, which is especially important for the delicate skin of infants.

[0003] Although camellia oil has many above-mentioned advantages, when it is applied to cosmetics such as creams, there are also some problems that need to be improved. Due to the high content of unsaturated fatty acids in camellia oil, its stability in the cream is poor. Especially during long-term storage, it is prone to oxidation, resulting in the yellowing of the cream color, which in turn affects its quality and effect. In addition, high and low temperature changes will also affect the texture and stability of the cream, resulting in poor user experience.

[0004] To overcome this problem, the following methods can be adopted in the existing technology. For example: First, by optimizing the product packaging design or storage environment of the cream, using dark glass bottles or opaque plastic bottles to reduce the influence of ultraviolet rays and prevent oil oxidation; adopting vacuum packaging, pump heads or pressing caps to reduce the chance of air contact and lower the oxidation risk; storing the product in a cool place or refrigerating it to avoid high temperature accelerating oil oxidation. Second, through the formula optimization and technological innovation of the cream, choosing more stable emulsifiers or using a compound emulsifier method to enhance the emulsification stability of the cream and reduce the phenomenon of oil-water separation; encapsulating camellia oil in microcapsules can isolate external environmental factors, reduce the oxidation rate, and at the same time slowly release active ingredients during use; using nanotechnology to prepare nanoemulsions can improve the dispersion uniformity and stability of oils, reduce particle aggregation, and improve the overall stability of the product. These above-mentioned methods can improve the stability of camellia oil cream to a certain extent, but how to further improve the comprehensive performance of the product and obtain a camellia oil cream with significant antioxidant, anti-aging and anti-wrinkle effects and stable storage performance at the same time is an urgent problem to be solved by the present invention. Summary of the Invention

[0005] The object of the present invention is to provide a camellia oil anti-wrinkle, anti-aging and antioxidant cream, which is composed of camellia oil, plant fermented active substances, glycerol, 1,3-butanediol, sorbitol, sodium hyaluronate, squalane, L-arginine, tocopherol, emulsifiers (PEG-7 glyceryl cocoate, cetearyl alcohol, glyceryl behenate), preservatives (ethylhexylglycerin, methylparaben), and water. This cream has excellent antioxidant, anti-aging and anti-wrinkle effects, is mild and moisturizing, and has stable storage performance. The present invention also provides a preparation method for the above-mentioned camellia oil cream, which is simple and easy to operate.

[0006] In order to achieve the above object, the present invention provides the following technical solutions:

[0007] The present invention provides a camellia oil anti-wrinkle, anti-aging and antioxidant cream, which comprises the following components:

[0008] Camellia oil 6-8 wt%, plant fermented active substances 1-3 wt%, glycerol 6-10 wt%, 1,3-butanediol 5-7 wt%, sorbitol 5-7 wt%, sodium hyaluronate 1-2 wt%, squalane 1-2 wt%, L-arginine 0.4-0.6 wt%, tocopherol 0.1-0.3 wt%, PEG-7 glyceryl cocoate 0.4-0.6 wt%, cetearyl alcohol 0.8-1.2 wt%, glyceryl behenate 0.8-1.2 wt%, ethylhexylglycerin 0.4-0.6 wt%, methylparaben 0.08-0.12 wt%, and deionized water as the balance.

[0009] The cream of the present invention uses camellia oil as the main antioxidant and anti-wrinkle component, and tocopherol and squalane as supplements. These components provide excellent antioxidant effects, promote collagen production, and reduce fine lines and wrinkles; camellia oil also provides deep nourishment and keeps the skin soft and smooth; tocopherol enhances the antioxidant effect and promotes the repair of damaged skin; squalane is similar to the natural oils of the skin, provides long-lasting moisturization and nourishment, and restores the natural oil balance of the skin; moisturizing and skin-nourishing components such as glycerol, 1,3-butanediol, sorbitol, sodium hyaluronate and L-arginine enhance the water retention ability, form a protective film, prevent water loss, and jointly achieve the effect of moisturizing and skin-nourishing; PEG-7 glyceryl cocoate, cetearyl alcohol and glyceryl behenate are used as emulsifiers and viscosity regulators to promote the stable mixing of oil and water, make the texture of the cream smooth and easy to apply, and promote absorption; ethylhexylglycerin and methylparaben are used as preservatives to inhibit the growth of bacteria, extend the shelf life, ensure the safety of the product, are suitable for various skin types, and provide high-efficiency anti-aging and moisturizing effects.

[0010] Preferably, the preparation method of the plant fermented active substances comprises the following steps:

[0011] Step (1): Grind the whole herb of Erigeron breviscapus and the whole herb of Plantago asiatica and sieve them to obtain raw material powder; subject the raw material powder to explosion puffing treatment for 4 - 6 min to obtain pre - treated raw material powder;

[0012] Step (2): Mix the pre - treated raw material powder evenly in deionized water, sterilize it, inoculate fermentation bacteria, and carry out constant - temperature fermentation for 30 - 40 h. After the fermentation is completed, heat and extract for 1 - 3 h, filter to obtain filtrate and filter residue, and reserve the filter residue; Concentrate the filtrate under reduced pressure to obtain active component A;

[0013] Step (3): Add the filter residue obtained in step (2) into an extraction tank, carry out supercritical extraction for 1 - 2 h to obtain an extract; Filter the extract and concentrate it under reduced pressure to obtain active component B; Mix active component A and active component B evenly to obtain plant - fermented active substance.

[0014] By adding the plant - fermented active substance prepared by the above - mentioned specific method, the present invention can effectively improve the stability of camellia oil, inhibit the oxidation of highly unsaturated acids in camellia oil into peroxides during storage due to the action of air, ultraviolet light, moisture and metal ions, and then decompose into volatile and odor - producing ketone / aldehyde acid substances, thereby preventing the phenomenon of rancidity. At the same time, it can also be used as a synergistic component to further improve the antioxidant and anti - aging effects of camellia oil.

[0015] The present invention preferably uses the whole herb of Erigeron breviscapus and the whole herb of Plantago asiatica as raw materials. Among them, Erigeron breviscapus is rich in antioxidant and anti - inflammatory components such as scutellarin, tanshinone, flavonol, dihydroflavone, coumarin, phytosterol, caffeoyl compound, polysaccharide, etc., and Plantago asiatica is rich in antioxidant and anti - inflammatory components such as rutin, quercetin, plantaginin, baicalein, vitamins, baicalin, triterpenoids, polysaccharide, etc. First, the plant raw materials are ground and subjected to explosion puffing pretreatment, making the fiber structure in the raw materials loose, which is more conducive to subsequent fermentation by fermentation bacteria and extraction of active components, thus ultimately improving the efficacy of the plant - fermented active substance; Then, Bacillus subtilis and Lactiplantibacillus plantarum are used for fermentation treatment. Not only can the synergistic effect of the two fermentation bacteria be utilized to optimize the fermentation process, thereby producing richer active components, but also the fiber structure of the raw materials can be further damaged, making the active ingredients easier to release, contributing to subsequent extraction and improving the extraction efficiency. Then, a combined extraction method of hot - water extraction and supercritical extraction is adopted. The hot - water extraction method is mainly used to extract water - soluble components such as flavonoids and polysaccharides, while the supercritical extraction method is mainly used to extract fat - soluble components such as flavonol, phytosterol, and terpenoids. The combination of these two extraction methods can maximize the retention of active components in plant raw materials. These active components can effectively inhibit the activity of unsaturated fatty acid degrading enzymes, delay oxidative rancidity, and improve the storage stability, antioxidant and anti - aging effects of camellia oil cream.

[0016] Furthermore, the preparation method of the plant - fermented active substance includes the following steps:

[0017] Step (1): Take the cleaned and dried whole herb of Erigeron breviscapus and the whole herb of Plantago asiatica, crush them and pass through a 100 - 300 mesh sieve to obtain raw material powder; place the raw material powder under the conditions of 1 - 2 MPa and 50 - 65 °C for air explosion treatment for 4 - 6 min to obtain pretreated raw material powder;

[0018] Step (2): Add the pretreated raw material powder to deionized water and mix evenly to obtain a material liquid, sterilize it at 115 - 121 °C for 15 - 20 min, inoculate fermentation bacteria after cooling to room temperature, carry out constant - temperature fermentation at 37 - 40 °C for 30 - 40 h. After the fermentation ends, heat and extract at 90 - 100 °C and 100 - 200 r / min for 1 - 3 h, filter to obtain a filtrate and filter residue, and reserve the filter residue; Concentrate the filtrate under reduced pressure to 1 - 3% of the original volume to obtain active component A;

[0019] Step (3): Add the filter residue obtained in step (2) to an extraction tank, carry out supercritical extraction at 30 - 40 Mpa and 50 - 60 °C for 1 - 2 h to obtain an extract; filter the extract and concentrate it under reduced pressure to 2 - 4% of the original volume to obtain active component B; Mix active component A and active component B evenly according to a weight ratio of 1:1 - 2 to obtain a plant - fermented active substance.

[0020] Preferably, in step (1), the weight ratio of the whole herb of Erigeron breviscapus to the whole herb of Plantago asiatica is 1 - 3:1.

[0021] Preferably, in step (2), the solid - liquid ratio of the pretreated raw material powder to deionized water is 1 g:8 - 12 mL.

[0022] Preferably, in step (2), the inoculation amount of the fermentation bacteria is 1 - 2×10 8 CFU / mL of the material liquid.

[0023] Preferably, in step (2), the fermentation bacteria are Bacillus subtilis and / or Lactobacillus plantarum.

[0024] Preferably, in step (2), the fermentation bacteria are composed of Bacillus subtilis and Lactobacillus plantarum, and the dosage ratio of Bacillus subtilis to Lactobacillus plantarum is 3 - 5:5.

[0025] Furthermore, the number of Bacillus subtilis is CICC 22063, and the number of Lactobacillus plantarum is CICC21796, both are purchased from the China Center for Industrial Culture Collection.

[0026] Preferably, in step (3), the extraction solvent for supercritical extraction is CO 2 fluid, and the CO 2 flow rate is 13 - 16 kg / h.

[0027] Preferably, in step (3), the extraction entrainer for supercritical extraction is an ethanol aqueous solution with a concentration of 85-95 wt%, and the entrainer flow rate is 0.4-0.6 g / min.

[0028] The present invention provides a method for preparing a camellia oil anti-wrinkle, anti-aging and antioxidant cream, comprising the following steps:

[0029] Add glycerol, 1,3-butanediol, sorbitol, sodium hyaluronate, ethylhexylglycerin, methylparaben to deionized water according to the component formula, stir at 50-65 °C and 200-400 r / min for 10-20 min, then successively add camellia oil, plant fermentation active substances, squalane, L-arginine, tocopherol, PEG-7 glyceryl cocoate, cetearyl alcohol, glyceryl behenate and continue stirring for 20-40 min, then homogenize at 1500-2500 r / min for 4-6 min, sterilize, and discharge to obtain the camellia oil anti-wrinkle, anti-aging and antioxidant cream.

[0030] Compared with the prior art, the advantages and beneficial effects of the present invention are as follows:

[0031] 1. The present invention provides a method for preparing a camellia oil anti-wrinkle, anti-aging and antioxidant cream. The camellia oil cream obtained by this method has stable storage performance, is mild and moisturizing, has good absorption effect, has excellent antioxidant, anti-aging and anti-wrinkle effects, and can significantly improve skin elasticity and fade wrinkles.

[0032] 2. The present invention uses camellia oil as the main antioxidant and anti-wrinkle component, tocopherol and squalane as auxiliary components, and glycerol, 1,3-butanediol, sorbitol, sodium hyaluronate and L-arginine as moisturizing and skin-nourishing components to enhance the water retention ability, form a protective film, prevent water loss, and jointly achieve the moisturizing and skin-nourishing effect. Through these components, excellent antioxidant effects are provided, collagen production is promoted, fine lines and wrinkles are reduced, and the skin is kept soft and smooth.

[0033] 3. By adding plant fermentation active substances prepared from Erigeron breviscapus and Plantago asiatica, the present invention can effectively improve the stability of camellia oil, inhibit the oxidation and rancidity of highly unsaturated acids in camellia oil during storage, and at the same time can also be used as a synergistic component to further improve the antioxidant and anti-aging effects of camellia oil. Detailed Embodiments

[0034] The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. All other embodiments obtained by those of ordinary skill in the art without creative efforts based on the embodiments of the present invention belong to the scope of protection of the present invention.

[0035] Example 1

[0036] This example provides a camellia oil anti-wrinkle, anti-aging and antioxidant cream, which is composed of the following components:

[0037] Camellia oil 7.5wt%, plant fermentation active substance 2.5wt%, glycerol 8wt%, 1,3-butanediol 6wt%, sorbitol 6wt%, sodium hyaluronate 1.5wt%, squalane 1.5wt%, L-arginine 0.5wt%, tocopherol 0.2wt%, PEG-7 glyceryl cocoate 0.5wt%, cetearyl alcohol 1wt%, glyceryl behenate 1wt%, ethylhexylglycerin 0.5wt%, methylparaben 0.1wt%, and deionized water is the balance.

[0038] The preparation method of the plant fermentation active substance includes the following steps:

[0039] Step (1): Take the whole herb of Erigeron breviscapus and the whole herb of Plantago asiatica that have been cleaned and dried, crush them through a 200-mesh sieve to obtain raw material powder. The weight ratio of the whole herb of Erigeron breviscapus to the whole herb of Plantago asiatica is 2:1; place the raw material powder under the conditions of 1.5 MPa and 60 °C for air explosion treatment for 5 min to obtain pre-treated raw material powder;

[0040] Step (2): Add the pre-treated raw material powder to deionized water according to a solid-liquid ratio of 1 g:10 mL, mix evenly to obtain a material liquid, sterilize it at 115 °C for 20 min, inoculate fermentation bacteria after cooling to room temperature, and the inoculation amount of the fermentation bacteria is 1.5×10 8 CFU / mL of the material liquid, carry out constant-temperature fermentation at 38 °C for 36 h. After the fermentation is completed, heat and extract it at 95 °C and 150 r / min for 2 h, filter to obtain a filtrate and a filter residue, and reserve the filter residue; concentrate the filtrate under reduced pressure to 2% of the original volume to obtain active component A; the fermentation bacteria are composed of Bacillus subtilis and Lactobacillus plantarum, and the dosage ratio of Bacillus subtilis to Lactobacillus plantarum is 4:5;

[0041] Step (3): Add the filter residue obtained in step (2) to an extraction tank, carry out supercritical extraction at 35 MPa and 55 °C for 1.5 h, the extraction solvent is CO 2 fluid, the CO 2 flow rate is 15 kg / h, the extraction entrainer is a 90wt% ethanol aqueous solution, and the entrainer flow rate is 0.5 g / min to obtain an extract; filter the extract and concentrate it under reduced pressure to 3% of the original volume to obtain active component B; mix active component A and active component B evenly according to a weight ratio of 1:1 to obtain a plant fermentation active substance. Among them, the strain number of Bacillus subtilis is CI CC 22063, and the strain number of Lactobacillus plantarum is CI CC 21796, both of which are purchased from the China Center for Industrial Culture Collection Management.

[0042] This embodiment provides a method for preparing a camellia oil anti-wrinkle, anti-aging and antioxidant cream, which includes the following steps:

[0043] Add glycerol, 1,3-butanediol, sorbitol, sodium hyaluronate, ethylhexylglycerin, and methylparaben to deionized water according to the group formula, stir at 60 °C and 300 r / min for 15 min, then sequentially add camellia oil, plant fermentation active substances, squalane, L-arginine, tocopherol, PEG-7 glyceryl cocoate, cetearyl alcohol, and glyceryl behenate and continue stirring for 30 min, then homogenize at 2000 r / min for 5 min, sterilize, and discharge to obtain the camellia oil anti-wrinkle, anti-aging and antioxidant cream.

[0044] Example 2

[0045] A camellia oil anti-wrinkle, anti-aging and antioxidant cream, which is composed of the following components:

[0046] Camellia oil 6 wt%, plant fermentation active substances 1 wt%, glycerol 6 wt%, 1,3-butanediol 5 wt%, sorbitol 5 wt%, sodium hyaluronate 1 wt%, squalane 1 wt%, L-arginine 0.4 wt%, tocopherol 0.1 wt%, PEG-7 glyceryl cocoate 0.4 wt%, cetearyl alcohol 0.8 wt%, glyceryl behenate 0.8 wt%, ethylhexylglycerin 0.4 wt%, methylparaben 0.08 wt%, and the balance is deionized water.

[0047] The preparation method of the plant fermentation active substances is the same as that in Example 1.

[0048] This embodiment provides a method for preparing a camellia oil anti-wrinkle, anti-aging and antioxidant cream, which includes the following steps:

[0049] Add glycerol, 1,3-butanediol, sorbitol, sodium hyaluronate, ethylhexylglycerin, and methylparaben to deionized water according to the group formula, stir at 50 °C and 200 r / min for 10 min, then sequentially add camellia oil, plant fermentation active substances, squalane, L-arginine, tocopherol, PEG-7 glyceryl cocoate, cetearyl alcohol, and glyceryl behenate and continue stirring for 20 min, then homogenize at 1500 r / min for 4 min, sterilize, and discharge to obtain the camellia oil anti-wrinkle, anti-aging and antioxidant cream.

[0050] Example 3

[0051] A camellia oil anti-wrinkle, anti-aging and antioxidant cream, which is composed of the following components:

[0052] 8 wt% camellia oil, 3 wt% plant fermentation active substance, 10 wt% glycerol, 7 wt% 1,3 - butanediol, 7 wt% sorbitol, 2 wt% sodium hyaluronate, 2 wt% squalane, 0.6 wt% L - arginine, 0.3 wt% tocopherol, 0.6 wt% PEG - 7 glyceryl cocoate, 1.2 wt% cetearyl alcohol, 1.2 wt% glyceryl behenate, 0.6 wt% ethylhexylglycerin, 0.12 wt% methylparaben, and the balance is deionized water.

[0053] The preparation method of the plant fermentation active substance is the same as that in Example 1.

[0054] This example provides a preparation method of a camellia oil anti - wrinkle, anti - aging and antioxidant cream, which includes the following steps:

[0055] Add glycerol, 1,3 - butanediol, sorbitol, sodium hyaluronate, ethylhexylglycerin, and methylparaben to deionized water according to the component formula, stir at 65 °C and 400 r / min for 20 min, then successively add camellia oil, plant fermentation active substance, squalane, L - arginine, tocopherol, PEG - 7 glyceryl cocoate, cetearyl alcohol, and glyceryl behenate and continue stirring for 40 min, then homogenize at 2500 r / min for 6 min, sterilize, and discharge to obtain the camellia oil anti - wrinkle, anti - aging and antioxidant cream.

[0056] Comparative Example 1

[0057] The difference between this comparative example and Example 1 is that step (1) in the preparation method of the plant fermentation active substance is different, specifically as follows: Take the cleaned and dried whole herb of Erigeron breviscapus, crush it through a 200 - mesh sieve to obtain raw material powder; Place the raw material powder under the conditions of 1.5 MPa and 60 °C for air - explosion treatment for 5 min to obtain pre - treated raw material powder.

[0058] Comparative Example 2

[0059] The difference between this comparative example and Example 1 is that step (1) in the preparation method of the plant fermentation active substance is different, specifically as follows: Take the cleaned and dried whole herb of Plantago asiatica, crush it through a 200 - mesh sieve to obtain raw material powder; Place the raw material powder under the conditions of 1.5 MPa and 60 °C for air - explosion treatment for 5 min to obtain pre - treated raw material powder.

[0060] Comparative Example 3

[0061] The difference between this comparative example and Example 1 is that step (1) in the preparation method of the plant fermentation active substance is different, specifically as follows: Take the cleaned and dried whole herb of Erigeron breviscapus and the whole herb of Plantago asiatica, crush them through a 200 - mesh sieve, and the weight ratio of the whole herb of Erigeron breviscapus to the whole herb of Plantago asiatica is 2:1; Obtain pre - treated raw material powder.

[0062] Comparative Example 4

[0063] The difference between this comparative example and Example 1 is as follows: Step (2) in the preparation method of the plant fermentation active substance is different, specifically as follows: The pretreated raw material powder is added to deionized water according to a solid-liquid ratio of 1 g:10 mL and mixed evenly, heated and extracted at 95°C and 150 r / min for 2 h, filtered to obtain a filtrate and a filter residue, and the filter residue is reserved; The filtrate is concentrated under reduced pressure to 2% of the original volume to obtain active component A.

[0064] Comparative Example 5

[0065] The difference between this comparative example and Example 1 is as follows: Step (2) in the preparation method of the plant fermentation active substance is different, specifically as follows: The pretreated raw material powder is added to deionized water according to a solid-liquid ratio of 1 g:10 mL and mixed evenly to obtain a liquid material, sterilized at 115°C for 20 min, inoculated with fermentation bacteria after cooling to room temperature, and the inoculation amount of the fermentation bacteria is 1.5×10 8 CFU / mL of the liquid material, fermented at a constant temperature of 38°C for 36 h. After the fermentation is completed, it is heated and extracted at 95°C and 150 r / min for 2 h, filtered to obtain a filtrate and a filter residue, and the filter residue is reserved; The filtrate is concentrated under reduced pressure to 2% of the original volume to obtain active component A; The fermentation bacteria are Bacillus subtilis.

[0066] Comparative Example 6

[0067] The difference between this comparative example and Example 1 is as follows: Step (2) in the preparation method of the plant fermentation active substance is different, specifically as follows: The pretreated raw material powder is added to deionized water according to a solid-liquid ratio of 1 g:10 mL and mixed evenly to obtain a liquid material, sterilized at 115°C for 20 min, inoculated with fermentation bacteria after cooling to room temperature, and the inoculation amount of the fermentation bacteria is 1.5×10 8 CFU / mL of the liquid material, fermented at a constant temperature of 38°C for 36 h. After the fermentation is completed, it is heated and extracted at 95°C and 150 r / min for 2 h, filtered to obtain a filtrate and a filter residue, and the filter residue is reserved; The filtrate is concentrated under reduced pressure to 2% of the original volume to obtain active component A; The fermentation bacteria are Lactiplantibacillus plantarum.

[0068] Comparative Example 7

[0069] The difference between this comparative example and Example 1 is as follows: The preparation method of the plant fermentation active substance is different, specifically as follows:

[0070] Step (1), Take the whole herb of Erigeron breviscapus and the whole herb of Plantago asiatica that have been cleaned and dried, crush them and pass through a 200-mesh sieve to obtain raw material powder, and the weight ratio of the whole herb of Erigeron breviscapus to the whole herb of Plantago asiatica is 2:1; Place the raw material powder under conditions of 1.5 MPa and 60°C for air explosion treatment for 5 min to obtain pretreated raw material powder;

[0071] Step (2): Add the pretreated raw material powder to deionized water at a solid-liquid ratio of 1 g: 10 mL, mix evenly to obtain a feed liquid, sterilize it at 115 °C for 20 min, inoculate the fermentation bacteria after cooling to room temperature, and the inoculation amount of the fermentation bacteria is 1.5×10 8 CFU / mL of the feed liquid, carry out constant-temperature fermentation at 38 °C for 36 h. After the fermentation is completed, heat and extract it at 95 °C and 150 r / min for 2 h, filter to obtain a filtrate and a filter residue, concentrate the filtrate under reduced pressure to 2% of the original volume. The fermentation bacteria are composed of Bacillus subtilis and Lactobacillus plantarum, and the dosage ratio of Bacillus subtilis to Lactobacillus plantarum is 4:5 to obtain the plant fermentation active substance.

[0072] Performance Test

[0073] 1. Stability determination: Place the anti-wrinkle, anti-aging, and antioxidant camellia oil creams prepared in Examples 1-3 and Comparative Examples 1-7 above under the following storage conditions for a period of time, and observe whether the cream changes color, whether it layers, and whether it produces an odor. If there is no color change, no layering, and no odor, it is recorded as qualified; if at least one of color change, layering, or odor appears, it is recorded as unqualified. The specific storage conditions are as follows: place at 4 °C for 2 months, 25 °C for 2 months, and 55 °C for 2 months. The results are shown in Table 1.

[0074] Table 1: Stability test results

[0075]

[0076]

[0077] 2. Anti-wrinkle and anti-aging determination: Invite 150 female volunteers with healthy skin (aged 35-45 years), randomly divide them into 10 groups, with 15 in each group. The subjects in each group are respectively corresponding to the anti-wrinkle, anti-aging, and antioxidant camellia oil creams prepared in Examples 1-3 and Comparative Examples 1-7 as samples. The experimental period is 2 months. During this period, the subjects apply the sample on the left half of the face, and do not apply any cosmetics on the right half of the face as a blank control, and use the sample once in the morning and once in the evening. At the beginning and end of the test, use a skin detector to detect and analyze the skin of the subjects, evaluate the improvement effect of the above creams on the skin elasticity of the cheeks and fine lines at the corners of the eyes (+ for increase, - for decrease), and take the average value. The results are shown in Table 2.

[0078] Table 2: Anti-wrinkle and anti-aging test results

[0079]

[0080]

[0081] From the above performance test results, it can be seen that the camellia oil facial creams prepared in Examples 1-3 have stable storage performance (high and low temperatures), can effectively improve skin elasticity, fade fine lines, and have good anti-wrinkle and anti-aging effects. In particular, the comprehensive performance of the camellia oil facial cream in Example 3 is the most prominent. This is because the present invention can effectively improve the stability of camellia oil by adding plant fermentation active substances prepared from Erigeron breviscapus and Plantago asiatica, and inhibit the oxidation of highly unsaturated acids in camellia oil into peroxides due to the action of air, ultraviolet rays, moisture and metal ions during storage, and then decompose into volatile and odorous ketone / aldehyde acid substances, resulting in rancidity. At the same time, it can also be used as a synergistic component to further improve the antioxidant and anti-aging effects of camellia oil.

[0082] In contrast, since Comparative Examples 1-7 did not adopt the necessary technical solutions, their corresponding performance tests were significantly worse than those of Examples 1-3. In Comparative Examples 1-3, the preferred raw material combination and pretreatment steps were not adopted in the preparation of plant fermentation active substances. In Comparative Examples 4-6, no specific fermentation bacteria were used for fermentation treatment. It can be seen from the results that this led to a decrease in the stability and anti-aging effect of the camellia oil facial cream. The above experimental results further prove the importance of the technical solutions defined in the present invention for its technical effects.

[0083] The above is the preferred embodiment of the present invention. It should be noted that for those of ordinary skill in the art, without departing from the principle of the present invention, several improvements and retouches can be made, and these improvements and retouches should also be regarded as the protection scope of the present invention.

Claims

1. A camellia oil anti-wrinkle, anti-aging and antioxidant facial cream, characterized in that: The facial cream comprises the following components: Camellia oil 6-8wt%, plant fermentation active matter 1-3wt%, glycerol 6-10wt%, 1,3-butylene glycol 5-7wt%, sorbitol 5-7wt%, sodium hyaluronate 1-2wt%, squalane 1-2wt%, L-arginine 0.4-0.6wt%, tocopherol 0.1-0.3wt%, PEG-7 glyceryl cocoate 0.4-0.6wt%, cetearyl alcohol 0.8-1.2wt%, glyceryl behenate 0.8-1.2wt%, ethylhexylglycerin 0.4-0.6wt%, methylparaben 0.08-0.12wt%, water as the balance; The method for preparing the plant fermentation active substance comprises the following steps: Step (1), taking the washed and dried whole herb of Erigeron breviscapus and whole herb of Plantago asiatica and crushing them through a 100-300 mesh sieve to obtain raw material powder; placing the raw material powder under the conditions of 1-2 MPa and 50-65° C. for gas explosion treatment for 4-6 minutes to obtain pretreated raw material powder; Step (2), adding the pretreated raw material powder to water and mixing evenly to obtain a feed liquid, sterilizing at 115-121° C. for 15-20 min, cooling to room temperature and inoculating fermentation bacteria, and fermenting at 37-40° C. for 30-40 h. After the fermentation is completed, heating and extracting at 90-100° C. and 100-200 r / min for 1-3 h, filtering to obtain a filtrate and a filter residue, and the filter residue is reserved; the filtrate is concentrated under reduced pressure to 1-3% of the original volume to obtain an active component A; Step (3), adding the filter residue obtained in step (2) to an extraction tank, and supercritically extracting at 30-40Mpa and 50-60°C for 1-2h to obtain an extract; filtering the extract, and concentrating under reduced pressure to 2-4% of the original volume to obtain an active component B; and uniformly mixing the active component A and the active component B at a weight ratio of 1:1-2 to obtain a plant fermentation active substance; The fermentation bacteria in step (2) are Bacillus subtilis and Bacillus plantarum.

2. The camellia oil anti-wrinkle, anti-aging and antioxidant facial cream according to claim 1, characterized in that: The weight ratio of the whole herb of Erigeron breviscapus to the whole herb of Plantago asiatica in step (1) is 1-3:

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3. The camellia oil anti-wrinkle, anti-aging and anti-oxidation cream according to claim 1, characterized in that: The inoculation amount of the fermentation bacteria in step (2) is 1-2×10 8 CFU / mL feed solution.

4. The camellia oil anti-wrinkle, anti-aging and antioxidant facial cream according to claim 1, characterized in that: The solid-to-liquid ratio of the pretreated raw material powder and water in step (2) is 1 g:8-12 mL.

5. The camellia oil anti-wrinkle, anti-aging and antioxidant facial cream according to claim 1, characterized in that: The extraction solvent of the supercritical extraction in step (3) is CO2 fluid, and the CO2 flow rate is 13-16kg / h.

6. The camellia oil anti-wrinkle, anti-aging and anti-oxidation facial cream according to claim 1, characterized in that: In step (3), the extraction entrainer of the supercritical extraction is 85-95wt% ethanol aqueous solution, and the entrainer flow rate is 0.4-0.6g / min.

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