Prebiotics-containing antibacterial gel for regulating microecological balance of female vagina

Abstract

The invention relates to a probiotic-containing antibacterial gel for regulating female vaginal micro-ecological balance. The probiotic-containing antibacterial gel comprises a probiotic ecological nutrition combined moisturizing liquid, a water-based gel matrix, a composite antibacterial agent, a plant extract, a pH regulator and purified water, the prebiotic ecological nutrition combined moisturizing liquid comprises plant collagen, alpha-glucan oligosaccharide, natural water-soluble yeast beta-glucan, inulin and the like, a hydrogel matrix comprises PCPVA anti-inflammatory hydrogel, lysozyme, lysostaphin and specially-made colloidal silver are added in a bacteriostatic agent, breakthrough is made from the source, and the prebiotic ecological nutrition combined moisturizing liquid has the advantages that the prebiotic ecological nutrition combined moisturizing liquid is suitable for being applied to the field of ecological and health care. A single microorganism killing mode is abandoned, a health repairing concept of increasing probiotics to reduce micro-ecological balance is advocated, comprehensive nutritional ingredients are provided for the probiotics at the private parts of women, and the vitality and the reproductive capacity of the probiotics are greatly enhanced, so that the growth of harmful bacteria is inhibited, the internal environment is purified, and through the four steps of cleaning, balancing, moistening and repairing, the health of women is improved. Therefore, micro-ecological balance of private parts is achieved, and inflammation troubles are avoided.

Description

Technical Field

[0001] The invention relates to the field of antibacterial gel, and in particular to an antibacterial gel containing prebiotics for regulating the microecological balance of female vagina. Background Art

[0002] A key challenge in the gynecology field is to maintain the health of women's private skin, which is easily invaded by bacteria due to its sensitivity. This invasion may lead to gynecological problems such as abnormal vaginal discharge, dryness, odor and itching. Currently, women usually use medication or ignore these symptoms. However, drug treatment may only be effective in the short term, and then the symptoms may recur or even worsen. On the other hand, ignoring these symptoms may worsen the originally mild flora imbalance problem, causing vaginal inflammation and ascending infection.

[0003] The root cause of gynecological inflammation and dryness of the private parts is the imbalance of the probiotic microecology. The medical community evaluates the immunity and self-cleaning ability of the private parts by testing the pH value of private secretions and the number of probiotics and lactobacilli. The female private parts constitute a microecological system. Under normal conditions, beneficial bacteria in the vagina should account for the vast majority, more than 95%. Lactobacilli play the role of guardian in this system. In a weakly acidic environment with a pH value of 3.8 to 4.4, they can effectively inhibit the growth of harmful bacteria. However, once the microecological balance of probiotics is destroyed, harmful bacteria will take the lead, causing a variety of inflammatory diseases and affecting women's normal life and activities. Summary of the invention

[0004] The technical purpose of the present invention is to solve the problems in the background technology and provide an antibacterial gel containing prebiotics for regulating the balance of female vaginal microecology.

[0005] The above technical objectives of the present invention are achieved through the following technical solutions: An antibacterial gel containing prebiotics for regulating the microecological balance of female vagina, the antibacterial gel comprising a prebiotic ecological nutrition combination moisturizing liquid, an aqueous gel matrix and a composite antibacterial agent, wherein the mass fraction of the prebiotic ecological nutrition combination moisturizing liquid is 2-6 parts, the mass fraction of the aqueous gel matrix is ​​1-5 parts, and the mass fraction of the composite antibacterial agent is 0.5-2 parts; Preferably, the prebiotic ecological nutrition combination moisturizing liquid comprises plant collagen and α-glucan oligosaccharide, wherein the mass fraction of the plant collagen component in the prebiotic ecological nutrition combination moisturizing liquid is 2-10 parts, and the mass fraction of the α-glucan oligosaccharide is 5-10 parts.

[0006] Under normal conditions, the flora in the vagina maintains a balance, and the presence of lactobacilli in particular is crucial for maintaining the stability of the host's vaginal environment. Lactobacilli bind to specific adhesin receptors on the surface of host cells through adhesins such as proteins and peptidoglycans on their surfaces, colonize in vaginal mucosal epithelial cells, and secrete extracellular polysaccharides to form biofilms during growth and reproduction. In addition, lactobacilli prevent the adhesion of pathogenic microorganisms through competitive exclusion mechanisms, and objectively inhibit the growth of other microorganisms by consuming a large amount of glycogen and nutrients.

[0007] In view of the key role of lactobacillus in maintaining the balance of vaginal microecology, the method of promoting lactobacillus growth by exogenous supplementation, i.e. vaginal microecology therapy, has been proved to have good clinical efficacy. This therapy not only avoids the side effects such as drug resistance, secondary infection and allergic reaction that antibiotics may cause, but also does not kill beneficial bacteria indiscriminately compared with antibiotics, thereby avoiding the further disorder of vaginal microecology and the extension of the recovery cycle. In addition, although some Chinese medicine preparations have bactericidal effects, they usually lack the ability to adjust and guide microbial balance. Therefore, the present invention is intended to provide a more scientific and effective treatment method for gynecological care by a novel prebiotic antibacterial gel.

[0008] The present invention breaks through from the root, abandons the single mode of killing microorganisms (excessive disinfection damages the internal environment and is prone to repeated infection), advocates the health repair concept of increasing probiotics to restore the balance of microecology, and provides comprehensive nutrients for probiotics in the female private parts, greatly enhancing the vitality and reproduction of probiotics, thereby inhibiting the growth of harmful bacteria and purifying the internal environment. After the four steps of "purification, balance, nourishment, and repair", the microecological balance of the private parts is achieved, keeping away from inflammation troubles.

[0009] Preferably, the aqueous gel matrix includes one or more of methyl cellulose, carboxymethyl cellulose, carboxyethyl cellulose, carboxypropyl methyl cellulose, gelatin, xanthan gum, pectin, carrageenan, and chitosan; further, the aqueous gel matrix also includes PCPVA hydrophilic anti-inflammatory hydrogel, the mass fraction of the PCPVA hydrogel is 0.1-1 part, and the rest is other aqueous gel matrices.

[0010] Preferably, the preparation method of the PCPVA hydrophilic anti-inflammatory hydrogel is: first, polyvinyl alcohol is dissolved in 60°C distilled water to obtain a 10% (w / w) polyvinyl alcohol solution; then, 1-5 parts by volume of a 10%-20% (w / w) sodium trimetaphosphate solution is added to 75-90 parts of the above polyvinyl alcohol solution and stirred until uniform, and after stirring evenly, 1-10 parts of a 15% (w / w) sodium hydroxide solution are added and continued to mix and stir evenly to obtain the PCPVA hydrophilic anti-inflammatory hydrogel.

[0011] The advantage of adopting the present invention is that the transparent and pure gel provided by the present invention has excellent thixotropy, can be deeply dispersed into the inner wall and the wrinkle area, remove the garbage toxins retained in the wrinkles, and solve a series of problems caused by the dirt toxins deposited in the wrinkles; it has a strong antibacterial effect, inhibits the source bacteria, establishes a probiotic microecology, fundamentally eliminates the problems of the female private parts, and fully repairs and maintains the female private parts.

[0012] With the present invention, it is advantageous that the use of PCPVA hydrogels can reduce inflammation and fibrosis by preventing fibroblast adhesion and fiber deposition, and capturing pro-inflammatory cytokines after implantation into the endometrial cavity.

[0013] Preferably, the prebiotic ecological nutrition combination moisturizing liquid also includes inulin, plant-derived fructans and fructose oligomers and purified water; the mass fraction of the inulin in the prebiotic ecological nutrition combination moisturizing liquid is 30-60 parts, the mass fraction of the plant-derived fructans and fructose oligomers is 15-30 parts, and the rest is purified water.

[0014] The invention has the advantages that the plant collagen selected in the ecological nutrition moisturizing liquid of the invention has extremely strong hydrophilicity and is a very good natural moisturizing ingredient. The plant collagen is not a general covering moisturizer and can promote the skin keratinocytes to generate natural moisturizing factors, exert moisturizing effects, penetrate and infiltrate the stratum corneum, make the skin more transparent and moisturized, form a skin self-protection mechanism, enhance skin elasticity, smooth skin fine lines, improve skin touch, and isolate external toxic and harmful substances; the inulin, fructan and fructose oligomers selected in the ecological nutrition moisturizing liquid promote the growth of epidermal probiotics through a selective nutrient supply mechanism, inhibit the growth of harmful bacteria through a competitive mechanism, establish and maintain a stable and healthy skin microecological system, maintain a healthy pH value of the skin, and reduce the occurrence of inflammation and allergic reactions.

[0015] Preferably, the composite antibacterial agent is two or three of lysozyme and lysostaphin; and further, when the composite antibacterial agent is two of lysozyme, lysostaphin and colloidal silver, the mass ratio of the two is 1:(1-2); when the composite antibacterial agent is three of lysozyme, lysostaphin and colloidal silver, the mass ratio of the three is 1:(1-2):(1-2).

[0016] The invention has the advantages that lysozyme can decompose insoluble mucopolysaccharides on the cell wall into soluble glycopeptides, and the cell wall ruptures to release the contents and dissolve the bacteria. Lysozyme can also directly bind to negatively charged viral proteins, form complexes with DNA, RNA, and apoproteins, and inactivate the virus.

[0017] The present invention has the advantage that lysostaphin is a Zn-containing 2+It is a metalloproteinase with endopeptidase activity, which can specifically hydrolyze the five glycine peptide bond bridge (peptide bond formed by the 2nd and 3rd Gly) of bacterial cell wall peptidoglycan, thereby quickly dissolving the bacterial cell wall and producing a wall-breaking lysing effect; the physicochemical properties, antibacterial mechanism of action, and in vitro and in vivo pharmacodynamics of lysostaphin have been widely studied. A large number of research results have shown that lysostaphin has the characteristics of high specificity, fast bactericidal speed, and low induction of drug resistance.

[0018] As a further preferred embodiment, the composite antibacterial agent comprises lysozyme, lysostaphin and colloidal silver, wherein the colloidal silver is locust bean gum / polyvinyl alcohol colloidal silver loaded with silver nanoparticles and is prepared by gel casting method, wherein the preparation method comprises: (1) preparing silver nanoparticle solution: mixing 60-90 parts of silver nitrate solution with a concentration of 2-5 mmol / L with 2-6 parts of fig leaf extract to prepare AgNPs; (2) preparing locust bean gum solution loaded with nanosilver: adding 1-5 parts of locust bean gum to (1) and mixing and stirring at room temperature for 24 hours; (3) preparing polyvinyl alcohol solution loaded with nanosilver: adding 2-5 parts of polyvinyl alcohol to the above (1) and stirring at room temperature for 24 hours; (4) mixing the hydrogels obtained in the steps (2) and (3) and drying them at 50°C to obtain the colloidal silver by gel casting method.

[0019] The invention has the advantages that AgNPs are synthesized by using fig leaf extract (FLE) and locust bean gum (LBG) / polyvinyl alcohol (PVA) loaded with silver nanoparticles (AgNPs) are prepared by gel casting method. Hydrogel, LBG / PVA / AgNP colloidal silver has good antibacterial properties against Gram-negative bacteria such as Escherichia coli and Pseudomonas aeruginosa and Gram-positive bacteria such as Staphylococcus aureus and Enterococcus faecalis; currently, the gel with colloidal silver as the raw material, which can produce strong bactericidal effects, and the inclusions of colloidal silver may have adverse effects on the environment and the human body. Therefore, the present invention not only adds plant components to the antibacterial gel, but also improves the preparation of colloidal silver. Nanosilver particles are prepared by reduction reaction of fig leaves and silver nitrate, and LBG / PVA / AgNP colloidal silver is prepared by this method. Not only the antibacterial properties of the composite antibacterial agent are optimized, but also the gel is neutral and does not destroy the acid-base balance of the human body. It can be quickly spread and evenly covered to achieve excellent antibacterial and anti-inflammatory effects, which helps to inhibit the growth of harmful bacteria in the body, relieve inflammation and scars, help to improve the living environment of human skin cells, promote the metabolism of skin tissue, and help to improve the therapeutic effect of daily vaginal care.

[0020] Preferably, the antibacterial gel further comprises a plant extract, a pH regulator and purified water, wherein the mass fraction of the plant extract is 1-5 parts, the mass fraction of the pH regulator is 0.5-2 parts, and the mass fraction of the purified water is 80-96 parts.

[0021] Preferably, the plant extracts include Cnidium monnieri extract, Phellodendron amurense extract, Aloe vera extract, Portulaca oleracea extract, Mushroom cube compound extract, Red clover extract, Bletilla striata root extract; the pH adjuster includes one or more of citric acid, sorbic acid, lactic acid, tartaric acid, and malic acid.

[0022] As a further preference, the prebiotic ecological nutrition combination moisturizing liquid also includes natural water-soluble yeast β-glucan, and the mass fraction of the natural water-soluble yeast β-glucan is 1-5 parts.

[0023] The invention has the advantages that the natural water-soluble yeast beta-glucan selected in the ecological nutrition moisturizing liquid of the invention can bind to the receptor CR3 of the yeast beta-glucan on the immune cell Langerhans cell in the epidermis, activate macrophages, produce various cytokines, epidermal growth factor, tumor necrosis factor and angiogenesis factor, etc., and enhance the immune ability of the skin; the yeast beta-glucan also has the effects of anti-inflammatory, anti-allergic, scavenging free radicals, moisturizing and accelerating the renewal of the stratum corneum, helps the skin resist various exogenous mechanical and chemical stimulations, promotes wound healing, and the increase of epidermal cell growth factor can increase the production of collagen and elastin, thereby improving the appearance of the skin and making fine wrinkles disappear.

[0024] Preferably, an antibacterial gel containing prebiotics for regulating the balance of female vaginal microecology comprises the following preparation steps: Step S1: adding an aqueous gel matrix to a stirring kettle, and then adding pure water; after the aqueous gel matrix is ​​swollen and dispersed, controlling the temperature at 70-85° C., stirring for 20-30 minutes, and stirring until the aqueous gel matrix is ​​completely dissolved into a gel state; Step S2: After the temperature of the solution in the stirring kettle in step S1 is lowered to room temperature, the above-mentioned prebiotic ecological nutrition combination moisturizing liquid, compound antibacterial agent and plant extract are added, and stirred for 15-25 minutes until the mixture is uniform; Step S3: Add a pH regulator to the reaction kettle in step S2, and stir at room temperature for 15-30 minutes until the pH of the solution in the stirred kettle is 4.5-6.5, thereby obtaining the antibacterial gel containing prebiotics for regulating the balance of female vaginal microecology.

[0025] As a further preference, the mass proportions of the components are: 2.5-4 parts of prebiotic ecological nutrition combination moisturizing liquid, 2.5-4 parts of aqueous gel matrix, 1.5-4.5 parts of plant extract, 0.8-1.2 parts of composite antibacterial agent, 0.6-1.2 parts of pH adjuster, and 86-92 parts of purified water.

[0026] In summary, the present invention has the following beneficial effects: 1. The antibacterial gel containing prebiotics for regulating the balance of female vaginal microecology provided by the scheme of the present invention can effectively inhibit the reproduction of harmful bacteria, maintain the clean and balanced environment in the vagina, eliminate symptoms such as burning sensation and itching and odor in the private parts, and at the same time quickly replenish water and nourish the private parts ecosystem, continuously and gently repair it, without irritating the mucous membrane, quickly repair the microcirculation of the female reproductive system, and reduce the incidence of gynecological inflammation; 2. By adding natural water-soluble yeast β-glucan, it can bind to the receptor CR3 of yeast β-glucan on the immune cells Langerhans cells in the epidermis, activate macrophages, produce various cytokines, epidermal growth factor, tumor necrosis factor and angiogenic factor, etc., and enhance the skin's immune ability; yeast β-glucan also has anti-inflammatory, anti-allergic, free radical scavenging, moisturizing and accelerated stratum corneum renewal, which helps the skin resist various exogenous mechanical and chemical stimuli and promote wound healing. The increase of epidermal cell growth factor can increase the production of collagen and elastin, thereby improving the appearance of the skin and eliminating fine wrinkles; 3. The plant collagen used in the ecological nutrition moisturizing liquid of the present invention has extremely strong hydrophilicity and is a very good natural moisturizing ingredient. Plant collagen is not a general covering moisturizer. At the same time, it can promote the skin keratinocytes to produce natural moisturizing factors, exert moisturizing effects, penetrate and infiltrate the stratum corneum, make the skin more transparent and moisturized, form a skin self-protection mechanism, enhance skin elasticity, smooth skin fine lines, improve skin touch, and isolate external toxic and harmful substances; inulin, fructan and fructose oligomers promote the growth of epidermal probiotics through a selective nutrient supply mechanism, inhibit the growth of harmful bacteria through a competitive mechanism, establish and maintain a stable and healthy skin micro-ecological system, maintain a healthy pH value of the skin, and reduce inflammation and allergic reactions. 4. By adding pure gel, PCPVA hydrophilic anti-inflammatory hydrogel is also added to the gel. PCPVA hydrogel can reduce inflammation and fibrosis by preventing fibroblast adhesion and fiber deposition, and capturing pro-inflammatory cytokines after implantation into the endometrial cavity, so that the antibacterial gel prepared by the present invention has excellent thixotropy, can be deeply dispersed into the inner wall and the wrinkle area, remove the garbage toxins retained in the wrinkles, and solve a series of problems caused by the dirt toxins deposited in the wrinkles; it has a strong antibacterial effect, inhibits source bacteria, establishes a probiotic microecology, fundamentally eliminates female private parts problems, and fully repairs and maintains female private parts; 5. The LBG / PVA / AgNP colloidal silver in the composite antibacterial agent is combined with the other two antibacterial agents, which not only optimizes the antibacterial properties of the composite antibacterial agent, but also makes the gel neutral without destroying the body's own acid-base balance. It can be quickly spread evenly on the surface to achieve excellent antibacterial and anti-inflammatory effects, which helps to inhibit the growth of harmful bacteria in the body, relieve inflammation and scars, help improve the living environment of human skin cells, promote the metabolism of skin tissue, and help improve the therapeutic effect of daily vaginal care; 6. The present invention breaks through from the root, abandons the single mode of killing microorganisms (excessive disinfection damages the internal environment and is prone to repeated infection), advocates the health repair concept of increasing probiotics to restore the balance of microecology, and provides comprehensive nutrients for probiotics in the female private parts, greatly enhancing the vitality and reproduction of probiotics, thereby inhibiting the growth of harmful bacteria and purifying the internal environment. After the four steps of "purification, balance, nourishment, and repair", the microecological balance of the private parts is achieved, keeping away from inflammation troubles. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] FIG1 is a schematic diagram of the components of an antibacterial gel containing prebiotics for regulating the balance of female vaginal microecology. DETAILED DESCRIPTION

[0028] The following specific embodiments are merely explanations of the present invention and are not limitations of the present invention. After reading this specification, those skilled in the art may make modifications to the embodiments without any creative contribution as needed. However, such modifications are protected by the patent law as long as they are within the scope of the claims of the present invention. Example 1

[0029] The mass fractions of each component in the antibacterial gel of this embodiment are: 2.5 parts of prebiotic ecological nutrition combination moisturizing liquid, 2.5 parts of aqueous gel matrix, 1.5 parts of plant extract, 0.8 parts of composite antibacterial agent, 0.6 parts of pH regulator, and 92.1 parts of purified water.

[0030] The prebiotic ecological nutrition combination moisturizing liquid contains 2 parts of plant collagen, 5 parts of α-glucan oligosaccharide, 30 parts of inulin, 15 parts of plant-derived fructan and fructose oligomers, 1 part of natural water-soluble yeast β-glucan, and the rest is purified water; The aqueous gel matrix contains 0.1 parts of PCPVA hydrogel and 2.4 parts of other aqueous gel matrices, which are methylcellulose, carrageenan, and chitosan; The plant extracts are Cnidium monnieri extract, Phellodendron amurense extract, Aloe vera extract, Portulaca oleracea extract, Mushroom cube compound extract, Red Clover extract, Bletilla striata root extract; The ratio of lysozyme, lysostaphin and colloidal silver in the composite antibacterial agent is 1:1:1; The antibacterial gel was prepared according to the following steps: Step S1: adding an aqueous gel matrix to a stirring tank, and then adding pure water; after the aqueous gel matrix is ​​swollen and dispersed, the temperature is controlled at 70° C. and stirred for 20 minutes until the aqueous gel matrix is ​​completely dissolved into a gel state; Step S2: After the temperature of the solution in the stirring kettle in step S1 is lowered to room temperature, the above-mentioned prebiotic ecological nutrition combination moisturizing liquid, compound antibacterial agent and plant extract are added, and stirred for 15 minutes until the mixture is uniform; Step S3: Add a pH regulator to the reaction kettle in step S2, and stir at room temperature for 15 minutes until the pH of the solution in the stirred kettle is 4.5, thereby obtaining the antibacterial gel containing prebiotics for regulating the balance of female vaginal microecology. Example 2

[0031] The mass fractions of each component in the antibacterial gel of this embodiment are: 3.1 parts of prebiotic ecological nutrition combination moisturizing liquid, 4 parts of aqueous gel matrix, 4.5 parts of plant extract, 1.2 parts of composite antibacterial agent, 1.2 parts of pH regulator, and 86 parts of purified water.

[0032] The prebiotic ecological nutrition combination moisturizing liquid contains 10 parts of plant collagen, 10 parts of α-glucan oligosaccharide, 45 parts of inulin, 30 parts of plant-derived fructan and fructose oligomers, 5 parts of natural water-soluble yeast β-glucan, and the rest is purified water; The aqueous gel matrix contains 1 part of PCPVA hydrogel and 3 parts of other aqueous gel matrices, and the other aqueous gel matrices are gelatin, xanthan gum, pectin, carrageenan, and chitosan; The plant extracts are Cnidium monnieri extract, Phellodendron amurense extract, Aloe vera extract, Portulaca oleracea extract, Mushroom cube compound extract, Red Clover extract, Bletilla striata root extract; The ratio of lysozyme, lysostaphin and colloidal silver in the composite antibacterial agent is 1:2:2; The antibacterial gel was prepared according to the following steps: Step S1: adding an aqueous gel matrix to a stirring tank, and then adding pure water; after the aqueous gel matrix is ​​swollen and dispersed, the temperature is controlled at 85° C. and stirred for 30 minutes until the aqueous gel matrix is ​​completely dissolved into a gel state; Step S2: After the temperature of the solution in the stirring kettle in step S1 is lowered to room temperature, the above-mentioned prebiotic ecological nutrition combination moisturizing liquid, compound antibacterial agent and plant extract are added, and stirred for 25 minutes until the mixture is uniform; Step S3: Add a pH regulator to the reaction kettle in step S2, and stir at room temperature for 30 minutes until the pH of the solution in the stirred kettle is 6.5, thereby obtaining the antibacterial gel containing prebiotics for regulating the microecological balance of female vagina. Example 3

[0033] The mass fractions of each component in the antibacterial gel of this embodiment are: 3 parts of prebiotic ecological nutrition combination moisturizing liquid, 3 parts of aqueous gel matrix, 4 parts of plant extract, 1 part of composite antibacterial agent, 1 part of pH regulator, and 88 parts of purified water.

[0034] The prebiotic ecological nutrition combination moisturizing liquid contains 5 parts of plant collagen, 8 parts of α-glucan oligosaccharide, 48 parts of inulin, 20 parts of plant-derived fructan and fructose oligomers, 2.5 parts of natural water-soluble yeast β-glucan, and the rest is purified water; The aqueous gel matrix contains 0.5 parts of PCPVA hydrogel and 2.5 parts of other aqueous gel matrices, and the other aqueous gel matrices are methylcellulose, carboxymethylcellulose, carboxyethylcellulose, carboxypropylmethylcellulose, carrageenan, and chitosan; The plant extracts are Cnidium monnieri extract, Phellodendron amurense extract, Aloe vera extract, Portulaca oleracea extract, Mushroom cube compound extract, Red Clover extract, Bletilla striata root extract; The ratio of lysozyme, lysostaphin and colloidal silver in the composite antibacterial agent is 1:1:2; The antibacterial gel was prepared according to the following steps: Step S1: adding an aqueous gel matrix to a stirring tank, and then adding pure water; after the aqueous gel matrix is ​​swollen and dispersed, the temperature is controlled at 80° C. and stirred for 25 minutes until the aqueous gel matrix is ​​completely dissolved into a gel state; Step S2: After the temperature of the solution in the stirring kettle in step S1 is lowered to room temperature, the above-mentioned prebiotic ecological nutrition combination moisturizing liquid, compound antibacterial agent and plant extract are added, and stirred for 20 minutes until the mixture is uniform; Step S3: Add a pH regulator to the reaction kettle in step S2, and stir at room temperature for 25 minutes until the pH of the solution in the stirred kettle is 5.5, thereby obtaining the antibacterial gel containing prebiotics for regulating the balance of female vaginal microecology. Example 4

[0035] The mass fractions of each component in the antibacterial gel of this embodiment are: 3.5 parts of prebiotic ecological nutrition combination moisturizing liquid, 3.5 parts of aqueous gel matrix, 3.5 parts of plant extract, 1 part of composite antibacterial agent, 1 part of pH regulator, and 87.5 parts of purified water.

[0036] The prebiotics eco-nutritional combination moisturizing liquid contains 8 parts of plant collagen, 6 parts of α-glucan oligosaccharides, 40 parts of inulin, 18 parts of plant-derived fructans and fructose oligomers, and 3 parts of natural water-soluble yeast β-glucan; The aqueous gel matrix contains 0.8 parts of PCPVA hydrogel and 2.7 parts of other aqueous gel matrices, and the other aqueous gel matrices are methylcellulose, carboxymethylcellulose, carboxyethylcellulose, carboxypropylmethylcellulose, gelatin, xanthan gum, pectin, carrageenan, and chitosan; The plant extracts are Cnidium monnieri extract, Phellodendron amurense extract, Aloe vera extract, Portulaca oleracea extract, Mushroom cube compound extract, Red Clover extract, Bletilla striata root extract; The ratio of lysozyme, lysostaphin and colloidal silver in the composite antibacterial agent is 1:2:1; The antibacterial gel was prepared according to the following steps: Step S1: adding an aqueous gel matrix to a stirring tank, and then adding pure water; after the aqueous gel matrix is ​​swollen and dispersed, the temperature is controlled at 80° C. and stirred for 25 minutes until the aqueous gel matrix is ​​completely dissolved into a gel state; Step S2: After the temperature of the solution in the stirring kettle in step S1 is lowered to room temperature, the above-mentioned prebiotic ecological nutrition combination moisturizing liquid, compound antibacterial agent and plant extract are added, and stirred for 20 minutes until the mixture is uniform; Step S3: Add a pH regulator to the reaction kettle in step S2, and stir at room temperature for 25 minutes until the pH of the solution in the stirred kettle is 5.5, thereby obtaining the antibacterial gel containing prebiotics for regulating the balance of female vaginal microecology. Example 5

[0037] The mass fractions of each component in the antibacterial gel of this embodiment are: 3.2 parts of prebiotic ecological nutrition combination moisturizing liquid, 2.8 parts of aqueous gel matrix, 3.5 parts of plant extract, 1.5 parts of composite antibacterial agent, 1 part of pH regulator, and 88 parts of purified water.

[0038] The prebiotic ecological nutrition combination moisturizing liquid contains 5 parts of plant collagen, 8 parts of α-glucan oligosaccharide, 48 parts of inulin, 20 parts of plant-derived fructan and fructose oligomers, 2.5 parts of natural water-soluble yeast β-glucan, and the rest is purified water; The aqueous gel matrix contains 0.5 parts of PCPVA hydrogel and 2.5 parts of other aqueous gel matrices, and the other aqueous gel matrices are methylcellulose, carboxymethylcellulose, carboxyethylcellulose, carboxypropylmethylcellulose, carrageenan, and chitosan; The plant extracts are Cnidium monnieri extract, Phellodendron amurense extract, Aloe vera extract, Portulaca oleracea extract, Mushroom cube compound extract, Red Clover extract, Bletilla striata root extract; The ratio of lysozyme, lysostaphin and colloidal silver in the composite antibacterial agent is 1:2:1; The preparation steps are the same as those in Example 4 above. Comparative Example 1

[0039] The mass proportions of the components in the antibacterial gel of this comparative example are: 3 parts of prebiotic ecological nutrition combination moisturizing liquid, 2.5 parts of aqueous gel matrix, 3.5 parts of plant extract, 1 part of composite antibacterial agent, 1 part of pH regulator, and 89 parts of purified water.

[0040] The prebiotic ecological nutrition combination moisturizing liquid contains 7 parts of plant collagen, 9.5 parts of α-glucan oligosaccharide, 52 parts of inulin, 24 parts of plant-derived fructan and fructose oligomers, 3.2 parts of natural water-soluble yeast β-glucan, and the rest is purified water; The aqueous gel matrix does not contain PCPVA hydrogel, and the aqueous gel matrix is ​​methylcellulose, carboxymethylcellulose, carboxyethylcellulose, carboxypropylmethylcellulose, carrageenan, and chitosan; The plant extracts are Cnidium monnieri extract, Phellodendron amurense extract, Aloe vera extract, Portulaca oleracea extract, Mushroom cube compound extract, Red Clover extract, Bletilla striata root extract; The ratio of lysozyme, lysostaphin and colloidal silver in the composite antibacterial agent is 1:1:2; The preparation steps are the same as those in the above embodiment. Comparative Example 2

[0041] The mass proportions of the components in the antibacterial gel of this comparative example are: 3 parts of prebiotic ecological nutrition combination moisturizing liquid, 3.2 parts of aqueous gel matrix, 3 parts of plant extract, 1 part of composite antibacterial agent, 1 part of pH regulator, and 88.8 parts of purified water.

[0042] The prebiotic ecological nutrition combination moisturizing liquid contains 5 parts of plant collagen, 8 parts of α-glucan oligosaccharide, 45 parts of inulin, 19 parts of plant-derived fructan and fructose oligomers, 1.7 parts of natural water-soluble yeast β-glucan, and the rest is purified water; The aqueous gel matrix does not contain PCPVA hydrogel, and other aqueous gel matrices are methylcellulose, carboxymethylcellulose, carboxyethylcellulose, carboxypropylmethylcellulose, gelatin, xanthan gum, pectin, carrageenan, and chitosan; The plant extracts are Cnidium monnieri extract, Phellodendron amurense extract, Aloe vera extract, Portulaca oleracea extract, Mushroom cube compound extract, Red Clover extract, Bletilla striata root extract; The ratio of lysozyme, lysostaphin and colloidal silver in the composite antibacterial agent is 1:1:2, wherein the colloidal silver is not prepared by the scheme of the present invention.

[0043] The preparation steps are the same as those in the above embodiment. Comparative Example 3

[0044] The mass parts of each component in the antibacterial gel of this comparative example are: 3 parts of prebiotic ecological nutrition combination moisturizing liquid, 3 parts of aqueous gel matrix, 4 parts of plant extract, 1 part of composite antibacterial agent, 1 part of pH regulator, and 88 parts of purified water.

[0045] The prebiotic ecological nutrition combination moisturizing liquid contains 5 parts of plant collagen, 8 parts of α-glucan oligosaccharide, 48 parts of inulin, 20 parts of plant-derived fructan and fructose oligomers, 2.5 parts of natural water-soluble yeast β-glucan, and the rest is purified water; The aqueous gel matrix does not contain PCPVA hydrogel, and other aqueous gel matrices are methylcellulose, carboxymethylcellulose, carboxyethylcellulose, carboxypropylmethylcellulose, gelatin, xanthan gum, pectin, carrageenan, and chitosan; The plant extracts are Cnidium monnieri extract, Phellodendron amurense extract, Aloe vera extract, Portulaca oleracea extract, Mushroom cube compound extract, Red Clover extract, Bletilla striata root extract; The ratio of lysozyme, lysostaphin and colloidal silver in the composite antibacterial agent is 1:1:2, wherein the colloidal silver is not prepared by the scheme of the present invention; The preparation steps are the same as those in the above embodiment. Comparative Example 4

[0046] The mass parts of each component in the antibacterial gel of this comparative example are: 3 parts of prebiotic ecological nutrition combination moisturizing liquid, 3 parts of aqueous gel matrix, 4 parts of plant extract, 1 part of composite antibacterial agent, 1 part of pH regulator, and 88 parts of purified water.

[0047] The prebiotic ecological nutrition combination moisturizing liquid contains 5 parts of plant collagen, 8 parts of α-glucan oligosaccharide, 48 parts of inulin, 20 parts of plant-derived fructan and fructose oligomers, 2.5 parts of natural water-soluble yeast β-glucan, and the rest is purified water; The aqueous gel matrix contains 0.5 parts of PCPVA hydrogel, and the other aqueous gel matrices are methylcellulose, carboxymethylcellulose, carboxyethylcellulose, carboxypropylmethylcellulose, gelatin, xanthan gum, pectin, carrageenan, and chitosan, with the following weight parts: The plant extracts are Cnidium monnieri extract, Phellodendron amurense extract, Aloe vera extract, Portulaca oleracea extract, Mushroom cube compound extract, Red Clover extract, Bletilla striata root extract; The ratio of lysozyme, lysostaphin and colloidal silver in the composite antibacterial agent is 1:1:2, wherein the colloidal silver is not prepared by the scheme of the present invention; The preparation steps are the same as those in the above embodiment. Comparative Example 5

[0048] The mass parts of each component in the antibacterial gel of this comparative example are: 3 parts of prebiotic ecological nutrition combination moisturizing liquid, 3 parts of aqueous gel matrix, 4 parts of plant extract, 1 part of composite antibacterial agent, 1 part of pH regulator, and 88 parts of purified water.

[0049] The prebiotic ecological nutrition combination moisturizing liquid contains 5 parts of plant collagen, 8 parts of α-glucan oligosaccharide, 48 parts of inulin, 20 parts of plant-derived fructan and fructose oligomers, 2.5 parts of natural water-soluble yeast β-glucan, and the rest is purified water; The aqueous gel matrix contains 0.5 parts of PCPVA hydrogel, and the other aqueous gel matrices are methylcellulose, carboxymethylcellulose, carboxyethylcellulose, carboxypropylmethylcellulose, gelatin, xanthan gum, pectin, carrageenan, and chitosan, with the following weight parts: The plant extracts are Cnidium monnieri extract, Phellodendron amurense extract, Aloe vera extract, Portulaca oleracea extract, Mushroom cube compound extract, Red Clover extract, Bletilla striata root extract; The ratio of lysozyme to lysostaphin in the composite antibacterial agent is 1:1; The preparation steps are the same as those in the above embodiment.

[0050] The antibacterial gels prepared in the above examples and comparative examples were subjected to antibacterial test, vaginal mucosal irritation test and volunteer trial of the gels.

[0051] Antibacterial test: Use a microsyringe to accurately measure 10uL of the antibacterial gel of Examples 1-5 and Comparative Examples 1-5 on a culture medium coated with E. coli, and culture at 37°C for 24 hours. Perform 5 parallel experiments for each sample. Use a spiral micrometer to measure the diameter of the antibacterial zone and take the average value.

[0052] Vaginal mucosal irritation experiment: The experimental subjects were 30 healthy female mice, each 3 mice were divided into a group, and Examples 1-5 and Comparative Examples 1-5 were 10 experimental groups. 20uL of the corresponding antibacterial gel was injected into the vagina of the mice, and 20uL of normal saline was injected into the control group. Each injection was 24 hours apart, and the injections were repeated 3 times. After 6 days, the vagina of the corresponding mice was taken out, cut longitudinally, and the physiological condition of the vaginal section was observed under a microscope to observe whether there were abnormal irritation phenomena such as discharge and erythema.

[0053] Results of the trial of the gel on volunteers: 100 volunteers were selected to try the antibacterial gel prepared in Example 3. After using it for a period of time, the changes in the vaginal flora were detected.

[0054] The results of the antibacterial test and vaginal mucosal irritation test are shown in Table 1: Table 1 Results of antibacterial test and vaginal mucosal irritation test

[0055] It can be seen from the antibacterial zone size results of Examples 1-5 and Comparative Examples 1-5 that Example 3 has the best antibacterial effect, with an antibacterial zone size of 11.5 mm, and the antibacterial gel is non-irritating to the vaginal mucosa. The vaginal flora detection found that the number of beneficial bacteria increased and the number of harmful bacteria decreased. In addition, the prepared antibacterial gel is avoided in the embodiment to achieve the purpose of sterilization while ignoring the balance of vaginal flora. The antibacterial effect is achieved while the effect of guiding the balance of microorganisms is taken into account. Compared with Comparative Examples 1-5, it can be concluded that the various components in the scheme of the present invention are reasonably and appropriately matched, and none of them can be missing. Lack of raw materials or changes in the number of portions will reduce the effect of the prepared gel.

Claims

1. An antibacterial gel containing prebiotics for regulating the balance of female vaginal microecology, characterized in that: The antibacterial gel comprises a prebiotic ecological nutrition combined moisturizing liquid, an aqueous gel matrix and a composite antibacterial agent, wherein the mass fraction of the prebiotic ecological nutrition combined moisturizing liquid is 2-6 parts, the mass fraction of the aqueous gel matrix is ​​1-5 parts, and the mass fraction of the composite antibacterial agent is 0.5-2 parts; The prebiotic eco-nutrition combined moisturizing liquid comprises plant collagen and α-glucan oligosaccharide, wherein the mass fraction of the plant collagen component in the prebiotic eco-nutrition combined moisturizing liquid is 2-10 parts, and the mass fraction of the α-glucan oligosaccharide is 5-10 parts; The aqueous gel matrix includes one or more of methyl cellulose, carboxymethyl cellulose, carboxyethyl cellulose, carboxypropyl methyl cellulose, gelatin, xanthan gum, pectin, carrageenan, and chitosan.

2. The antibacterial gel containing prebiotics for regulating the microecological balance of female vagina according to claim 1, characterized in that: The preparation method of the PCPVA hydrophilic anti-inflammatory hydrogel is as follows: first, polyvinyl alcohol is dissolved in 60° C. distilled water to obtain a 10% (w / w) polyvinyl alcohol solution; then, 1-5 parts by volume of a 10%-20% (w / w) sodium trimetaphosphate solution is added to 75-90 parts of the above polyvinyl alcohol solution and stirred until uniform; after stirring uniformly, 1-10 parts of a 15% (w / w) sodium hydroxide solution are added and continued to mix and stir uniformly to obtain the PCPVA hydrophilic anti-inflammatory hydrogel.

3. The antibacterial gel containing prebiotics for regulating the microecological balance of female vagina according to claim 1, characterized in that: The prebiotic ecological nutrition combination moisturizing liquid also includes inulin, plant-derived fructans and fructose oligomers and purified water; the mass fraction of the inulin in the prebiotic ecological nutrition combination moisturizing liquid is 30-60 parts, the mass fraction of the plant-derived fructans and fructose oligomers is 15-30 parts, and the rest is purified water; the aqueous gel matrix also includes PCPVA hydrophilic anti-inflammatory hydrogel, the mass fraction of the PCPVA hydrogel is 0.1-1 part, and the rest is other aqueous gel matrices.

4. The antibacterial gel containing prebiotics for regulating the microecological balance of female vagina according to claim 1, characterized in that: The composite antibacterial agent is two or three of lysozyme, lysostaphin and colloidal silver; and further, when the composite antibacterial agent is two of lysozyme, lysostaphin and colloidal silver, the mass ratio of the two is 1:(1-2); when the composite antibacterial agent is three of lysozyme, lysostaphin and colloidal silver, the mass ratio of the three is 1:(1-2):(1-2).

5. The antibacterial gel containing prebiotics for regulating the microecological balance of female vagina according to claim 4, characterized in that: Furthermore, the composite antibacterial agent comprises lysozyme, lysostaphin and colloidal silver, wherein the colloidal silver is locust bean gum / polyvinyl alcohol colloidal silver loaded with silver nanoparticles and is prepared by gel casting method, wherein the preparation method comprises: (1) preparing silver nanoparticle solution: mixing 60-90 parts of silver nitrate solution with a concentration of 2-5 mmol / L with 2-6 parts of fig leaf extract to prepare AgNPs; (2) preparing locust bean gum solution loaded with nanosilver: adding 1-5 parts of locust bean gum to (1) and mixing and stirring at room temperature for 24 hours; (3) preparing polyvinyl alcohol solution loaded with nanosilver: adding 2-5 parts of polyvinyl alcohol to (1) and stirring at room temperature for 24 hours; (4) mixing the hydrogels obtained in the steps (2) and (3) and drying them at 50°C to obtain the colloidal silver by gel casting method.

6. The antibacterial gel containing prebiotics for regulating the microecological balance of female vagina according to claim 1, characterized in that: The antibacterial gel also includes plant extract, pH regulator and purified water. The mass fraction of the plant extract is 1-5 parts, the mass fraction of the pH regulator is 0.5-2 parts, and the mass fraction of the purified water is 80-96 parts.

7. The antibacterial gel containing prebiotics for regulating the microecological balance of female vagina according to claim 6, characterized in that: The plant extracts include Cnidium monnieri extract, Phellodendron amurense extract, Aloe vera extract, Portulaca oleracea extract, Mushroom cube compound extract, Red Clover extract, Bletilla striata root extract; the pH adjuster includes one or more of citric acid, sorbic acid, lactic acid, tartaric acid, and malic acid.

8. The antibacterial gel containing prebiotics for regulating the microecological balance of female vagina according to claim 1, characterized in that: The prebiotic ecological nutrition combination moisturizing liquid also includes natural water-soluble yeast β-glucan, and the mass fraction of the natural water-soluble yeast β-glucan is 1-5 parts.

9. An antibacterial gel containing prebiotics for regulating the balance of female vaginal microecology, characterized in that: The antibacterial gel comprises the following preparation steps: Step S1: adding an aqueous gel matrix to a stirring tank, and then adding pure water; after the aqueous gel matrix is ​​swollen and dispersed, controlling the temperature at 70-85° C., stirring for 20-30 minutes, and stirring until the aqueous gel matrix is ​​completely dissolved into a gel state; Step S2: After the temperature of the solution in the stirring kettle in step S1 is lowered to room temperature, the above-mentioned prebiotic ecological nutrition combination moisturizing liquid, compound antibacterial agent and plant extract are added, and stirred for 15-25 minutes until the mixture is uniform; Step S3: Add a pH regulator to the reaction kettle in step S2, and stir at room temperature for 15-30 minutes until the pH of the solution in the stirred kettle is 4.5-6.5, thereby obtaining the antibacterial gel containing prebiotics for regulating the balance of female vaginal microecology.

10. The antibacterial gel containing prebiotics for regulating the microecological balance of female vagina according to claim 9, characterized in that: Preferably, the mass proportions of the components are: 2.5-4 parts of prebiotic ecological nutrition combination moisturizing liquid, 2.5-4 parts of aqueous gel matrix, 1.5-4.5 parts of plant extract, 0.8-1.2 parts of composite antibacterial agent, 0.6-1.2 parts of pH adjuster, and 86-92 parts of purified water.

Images