A composite plant growth regulator and its preparation method and application

Abstract

The invention discloses a composite plant growth regulator and a preparation method and application thereof, belonging to the field of plant growth regulators. The composite plant growth regulator comprises the following main components: sea buckthorn oil, brown cyanidin, triacontanol, uniconazole, and salicylic acid. The plant growth regulator of the invention adds sea buckthorn oil and brown cyanidin, and these two components are compounded with triacontanol, uniconazole and salicylic acid to form a composite plant growth regulator. The synergistic effect of multiple substances increases the number of effective panicles, the number of panicles and the thousand-grain weight of rice plants on the one hand, thereby increasing the yield; on the other hand, the internode length of the base of the rice is shortened, the plant height is shortened, the stalk is promoted to be thick, and the lodging resistance of the rice is enhanced. The plant growth regulator of the invention can be used alone as a plant growth regulator for increasing rice yield, reducing plant height and improving lodging resistance. In addition, the plant growth regulator of the invention has a wide source, low cost, simple preparation method, convenient use, and is easy to promote.

Description

Technical Field

[0001] The invention belongs to the technical field of plant growth regulators, and specifically relates to a composite plant growth regulator and a preparation method and application thereof. Background Art

[0002] Rice is one of the important staple food crops in my country. With the improvement of people's living standards, producing high-quality and safe rice has gradually become the main development goal of the rice industry. However, in recent years, disastrous weather has occurred frequently, and lodging is still one of the important factors limiting the high-quality and high-yield of rice. Rice lodging not only reduces the efficiency of mechanized harvesting, but also aggravates its diseases and pests and deteriorates its quality. There are many reasons for rice lodging, including both internal factors such as the variety's own lodging resistance, such as plant height, center of gravity height, stem characteristics, etc.; and external factors such as natural environmental conditions and field management measures, such as extreme weather such as strong winds and heavy rains, and cultivation methods, planting density, fertilizer and water management, and pest and disease control. Rice dwarf genes are widely used in the selection and breeding of lodging-resistant varieties, but traditional dwarf varieties have less biomass accumulation, which has greatly limited the increase in yield. In recent years, the breeding and planting of rice varieties such as large panicle super high-yield varieties and super rice have also significantly increased the pressure of rice lodging resistance. In addition, in order to reduce costs, rice production has gradually developed towards mechanization and light simplification, which has also put forward higher requirements for rice lodging resistance. Therefore, lodging resistance has become one of the key traits in rice breeding and cultivation. Agronomists have proposed many methods to prevent rice lodging. In addition to measures such as variety characteristics, cultivation conditions, fertilizer and water management, plant physiologists have proposed regulatory measures to prevent and control rice lodging using chemical active substances. Its mechanism of action is to enhance rice lodging resistance by improving the external plant shape and internal physiological functions related to lodging. The use of plant growth regulators (PGRs) has greater advantages than traditional technical measures, with the characteristics of small dosage, fast action, and obvious effect. Therefore, it is widely used in high-yield, high-quality and efficient rice cultivation.

[0003] Plant growth regulators are exogenous active substances produced by artificial synthesis, artificial extraction or biological fermentation. They have similar physiological activities to endogenous plant hormones, or can affect the synthesis, transportation, metabolism and physiological effects of endogenous plant hormones. Plant growth regulators have the functions of promoting seed germination, rooting, flowering, etc., can enhance the ability of plants to resist cold, drought, salinity and pests and diseases, and can also improve crop yield and quality. They have the advantages of low cost, small dosage and significant effect, and are widely used in agricultural production. Reasonable spraying of plant regulators is an effective cultivation measure to improve the resistance of rice plants to lodging, prevent lodging, increase yield and improve quality.

[0004] Seabuckthorn oil, also known as vinegar willow oil, vinegar willow oil, acid thorn oil, black thorn oil, is a precious natural oil extracted from the fruit of the natural plant seabuckthorn. In addition to unsaturated fatty acids, seabuckthorn oil also contains more than 100 biologically active substances such as flavonoids, vitamins, phytosterols, trace elements and α-tocopherol; cyanidin is a flavonoid active ingredient contained in the traditional Chinese medicine wormwood. These two substances have excellent medicinal value in the medical field. However, there is no research and report on the use of seabuckthorn oil and cyanidin to promote plant growth. Summary of the invention

[0005] The technical problem to be solved by the present invention is to provide a composite plant growth regulator to achieve the synergistic improvement of rice yield increase and lodging resistance.

[0006] In order to achieve the above technical objectives, the technical solution adopted by the present invention is:

[0007] A composite plant growth regulator comprises the following main ingredients: sea buckthorn oil, cyanidin, triacontanol, uniconazole and salicylic acid.

[0008] Wherein, the preparation method of seabuckthorn oil is:

[0009] (1) The whole seabuckthorn fruit was dried in an oven at 40°C for 24 h, and then crushed at 4000 r / min for 3 s to obtain seabuckthorn fruit powder. The water content of the seabuckthorn fruit powder was adjusted to 20%, and then stirred evenly. The mixture was placed on a microwave turntable and microwaved for 2 minutes to obtain a mixture A.

[0010] (2) Add a composite hydrolase solution containing pectin lyase and Bacillus subtilis fibrinolytic enzyme to mixture A, perform enzymatic hydrolysis at 40-50°C for 4 h, perform solid-liquid separation by centrifugation, and remove the lower precipitate; weigh the lower precipitate, add n-ethane, stir and extract at 45°C for 1 h, filter, and remove n-ethane from the filtrate by rotary evaporation and nitrogen blowing to obtain sea buckthorn oil.

[0011] Furthermore, the whole seabuckthorn fruit in step (1) is a dried fruit; in step (2), the mass-volume ratio of mixture A to the composite hydrolase solution is 1kg:1L, the composite hydrolase solution contains 18-24g / L pectin lyase and 5-8g / L Bacillus subtilis fibrinolytic enzyme, the enzyme activity of pectin lyase is 400,000 / g, and the enzyme activity of Bacillus subtilis fibrinolytic enzyme is 100,000 / g; in step (2), the weight-volume ratio of the lower layer precipitate and n-ethane is 1kg:8L.

[0012] Furthermore, the concentration of seabuckthorn oil is 50-200 mg / L; the concentration of cyanidin is 5-50 mg / L; the concentration of triacontanol is 0.2-0.4 mg / L; the concentration of uniconazole is 40-50 mg / L; and the concentration of salicylic acid is 10-30 mg / L.

[0013] Furthermore, the concentration of seabuckthorn oil is 100 mg / L; the concentration of cyanidin is 25 mg / L; the concentration of triacontanol is 0.3 mg / L; the concentration of uniconazole is 40 mg / L; and the concentration of salicylic acid is 20 mg / L.

[0014] Furthermore, the composite plant growth regulator is a liquid preparation.

[0015] A method for preparing a composite plant growth regulator is prepared by the following steps:

[0016] (1) Take a dilution container, dissolve Tween-80 in 600 mL of hot water to obtain an emulsifier solution, weigh the mass of seabuckthorn oil according to the concentration used, add it to the emulsifier solution and stir until uniform to obtain a seabuckthorn oil emulsion, and cool it to room temperature for use;

[0017] (2) Calculate the weight of cyanidin, triacontanol and uniconazole according to the concentration used, weigh cyanidin, triacontanol and uniconazole and dissolve them in ethanol. The total amount of ethanol used shall not exceed 50 ml / L after the solution is fixed to volume; add the dissolved cyanidin solution, triacontanol solution and uniconazole solution into the container in turn and stir evenly;

[0018] (3) Finally, salicylic acid is weighed according to the concentration to be used, added into a container, stirred until dissolved, and then water is added to make up the volume to obtain a composite plant growth regulator.

[0019] Furthermore, in the step (1), the mass ratio of seabuckthorn oil to Tween-80 is 1:1.

[0020] The materials used in the present invention are all commercially available.

[0021] The invention also provides the application of the composite plant growth regulator in increasing crop yield and resisting lodging.

[0022] Furthermore, the crop is rice.

[0023] Furthermore, the composite plant growth regulator is used in the form of foliar spraying.

[0024] Beneficial Effects

[0025] The present invention adds sea buckthorn oil and cyanidin, and these two ingredients are compounded with triacontanol, uniconazole and salicylic acid to form a composite plant growth regulator. The synergistic effect of multiple substances can significantly promote the growth and development of crops and increase yield. The compounding of multiple ingredients, on the one hand, promotes the growth and development of rice plants by promoting the absorption of nutrients, increases the number of effective tillers, thereby increasing the number of effective panicles, the number of panicles and the thousand-grain weight; on the other hand, it significantly increases the chlorophyll content in the leaves, enhances photosynthesis, promotes the transport of photosynthesis to grains, and increases yield. In addition, the composite plant growth regulator of the present invention shortens the internode length of the base of rice, shortens the plant height, promotes the sturdiness of the stems, enhances the lodging resistance of rice, and achieves a synergistic improvement of rice yield and lodging resistance.

[0026] The plant growth regulator of the present invention can be used alone as a plant growth regulator for increasing rice yield, reducing plant height and improving lodging resistance. In addition, the plant growth regulator of the present invention has a wide source, low cost, simple preparation method, convenient use and easy promotion. DETAILED DESCRIPTION

[0027] The technical solution of the present invention is further described below in conjunction with specific embodiments, but is not limited thereto.

[0028] Example 1

[0029] A composite plant growth regulator comprises the following main ingredients: sea buckthorn oil, cyanidin, triacontanol, uniconazole and salicylic acid.

[0030] Wherein, the preparation method of seabuckthorn oil is:

[0031] (1) The whole dried seabuckthorn fruit was dried in an oven at 40°C for 24 h, and then crushed at 4000 r / min for 3 s to obtain seabuckthorn fruit powder. The water content of the seabuckthorn fruit powder was adjusted to 20%, and then stirred evenly. The mixture was placed on a microwave turntable and microwaved for 2 minutes to obtain a mixture A.

[0032] (2) Take 10 kg of mixture A and add 10 L of a composite hydrolase solution containing pectin lyase and Bacillus subtilis fibrinolytic enzyme (18 g / L of pectin lyase with an enzyme activity of 400,000 / g and 5 g / L of Bacillus subtilis fibrinolytic enzyme with an enzyme activity of 100,000 / g), perform enzymatic hydrolysis at 40°C for 4 h, perform solid-liquid separation by centrifugation, remove the upper solution and retain the lower precipitate, weigh 1 kg of the lower precipitate, add 8 L of n-ethane, place in a constant temperature water bath at 45°C, stir and extract for 1 h, filter, and remove n-ethane from the filtrate by rotary evaporation and nitrogen blowing to obtain sea buckthorn oil.

[0033] The concentration of seabuckthorn oil is 50 mg / L; the concentration of cyanidin is 5 mg / L; the concentration of triacontanol is 0.2 mg / L; the concentration of uniconazole is 40 mg / L; and the concentration of salicylic acid is 10 mg / L.

[0034] A method for preparing a composite plant growth regulator is prepared by the following steps:

[0035] (1) Take a dilution container, dissolve Tween-80 in 600 mL of hot water to obtain an emulsifier solution, weigh the mass of seabuckthorn oil according to the concentration used, add it to the emulsifier solution and stir until uniform to obtain a seabuckthorn oil emulsion, and cool it to room temperature for use;

[0036] (2) Calculate the weight of cyanidin, triacontanol and uniconazole according to the concentration used, weigh cyanidin, triacontanol and uniconazole and dissolve them in ethanol. The total amount of ethanol used shall not exceed 50 ml / L after the solution is fixed to volume; add the dissolved cyanidin solution, triacontanol solution and uniconazole solution into the container in turn and stir evenly;

[0037] (3) Finally, salicylic acid is weighed according to the concentration to be used, added into a container, stirred until dissolved, and then water is added to make up the volume to obtain a composite plant growth regulator.

[0038] Example 2

[0039] A composite plant growth regulator comprises the following main ingredients: sea buckthorn oil, cyanidin, triacontanol, uniconazole and salicylic acid.

[0040] Wherein, the preparation method of seabuckthorn oil is:

[0041] (1) The whole dried seabuckthorn fruit was dried in an oven at 40°C for 24 h, and then crushed at 4000 r / min for 3 s to obtain seabuckthorn fruit powder. The water content of the seabuckthorn fruit powder was adjusted to 20%, and then stirred evenly. The mixture was placed on a microwave turntable and microwaved for 2 minutes to obtain a mixture A.

[0042] (2) Take 10 kg of mixture A and add 10 L of a composite hydrolase solution containing pectin lyase and Bacillus subtilis fibrinolytic enzyme (20 g / L of pectin lyase with an enzyme activity of 400,000 / g and 7 g / L of Bacillus subtilis fibrinolytic enzyme with an enzyme activity of 100,000 / g), perform enzymatic hydrolysis at 45°C for 4 h, perform solid-liquid separation by centrifugation, remove the upper solution and retain the lower precipitate, weigh 1 kg of the lower precipitate, add 8 L of n-ethane, place in a constant temperature water bath at 45°C, stir and extract for 1 h, filter, and remove n-ethane from the filtrate by rotary evaporation and nitrogen blowing to obtain sea buckthorn oil.

[0043] The concentration of seabuckthorn oil is 100 mg / L; the concentration of cyanidin is 25 mg / L; the concentration of triacontanol is 0.3 mg / L; the concentration of uniconazole is 40 mg / L; and the concentration of salicylic acid is 20 mg / L.

[0044] A method for preparing a composite plant growth regulator is prepared by the following steps:

[0045] (1) Take a dilution container and dissolve Tween-80 in 600 mL of hot water to obtain an emulsifier solution. Then weigh the mass of seabuckthorn oil according to the concentration to be used and add it to the emulsifier solution and stir until uniform to obtain a seabuckthorn oil emulsion. Cool it to room temperature for later use.

[0046] (2) Calculate the weight of cyanidin, triacontanol and uniconazole according to the concentration used, weigh cyanidin, triacontanol and uniconazole and dissolve them in ethanol. The total amount of ethanol used shall not exceed 50 ml / L after the solution is fixed to volume; add the dissolved cyanidin solution, triacontanol solution and uniconazole solution into the container in turn and stir evenly;

[0047] (3) Finally, salicylic acid is weighed according to the concentration to be used, added into a container, stirred until dissolved, and then water is added to make up the volume to obtain a composite plant growth regulator.

[0048] Example 3

[0049] A composite plant growth regulator comprises the following main ingredients: sea buckthorn oil, cyanidin, triacontanol, uniconazole and salicylic acid.

[0050] Wherein, the preparation method of seabuckthorn oil is:

[0051] (1) The whole dried seabuckthorn fruit was dried in an oven at 40°C for 24 h, and then crushed at 4000 r / min for 3 s to obtain seabuckthorn fruit powder. The water content of the seabuckthorn fruit powder was adjusted to 20%, and then stirred evenly. The mixture was placed on a microwave turntable and microwaved for 2 minutes to obtain a mixture A.

[0052] (2) Take 10 kg of mixture A and add 10 L of a composite hydrolase solution containing pectin lyase and Bacillus subtilis fibrinolytic enzyme (20 g / L of pectin lyase with an enzyme activity of 400,000 / g and 7 g / L of Bacillus subtilis fibrinolytic enzyme with an enzyme activity of 100,000 / g), perform enzymatic hydrolysis at 50°C for 4 h, perform solid-liquid separation by centrifugation, remove the upper solution and retain the lower precipitate, weigh 1 kg of the lower precipitate, add 8 L of n-ethane, place in a constant temperature water bath at 45°C, stir and extract for 1 h, filter, and remove n-ethane from the filtrate by rotary evaporation and nitrogen blowing to obtain sea buckthorn oil.

[0053] The concentration of seabuckthorn oil is 200 mg / L; the concentration of cyanidin is 50 mg / L; the concentration of triacontanol is 0.4 mg / L; the concentration of uniconazole is 50 mg / L; and the concentration of salicylic acid is 30 mg / L.

[0054] A method for preparing a composite plant growth regulator is prepared by the following steps:

[0055] (1) Take a dilution container, dissolve Tween-80 in 600 mL of hot water to obtain an emulsifier solution, weigh the mass of seabuckthorn oil according to the concentration used, add it to the emulsifier solution and stir until uniform to obtain a seabuckthorn oil emulsion, and cool it to room temperature for use;

[0056] (2) Calculate the weight of cyanidin, triacontanol and uniconazole according to the concentration used, weigh cyanidin, triacontanol and uniconazole and dissolve them in ethanol. The total amount of ethanol used shall not exceed 50 ml / L after the solution is fixed to volume; add the dissolved cyanidin solution, triacontanol solution and uniconazole solution into the container in turn and stir evenly;

[0057] (3) Finally, salicylic acid is weighed according to the concentration to be used, added into a container, stirred until dissolved, and then water is added to make up the volume to obtain a composite plant growth regulator.

[0058] Comparative Example 1

[0059] A composite plant growth regulator comprises the following main ingredients: sea buckthorn oil, cyanidin, triacontanol, uniconazole and salicylic acid.

[0060] The concentration of seabuckthorn oil is 300 mg / L; the concentration of cyanidin is 25 mg / L; the concentration of triacontanol is 0.3 mg / L; the concentration of uniconazole is 40 mg / L; and the concentration of salicylic acid is 20 mg / L.

[0061] Comparative Example 2

[0062] A composite plant growth regulator comprises the following main ingredients: sea buckthorn oil, cyanidin, triacontanol, uniconazole and salicylic acid.

[0063] The concentration of seabuckthorn oil is 100 mg / L; the concentration of cyanidin is 60 mg / L; the concentration of triacontanol is 0.3 mg / L; the concentration of uniconazole is 40 mg / L; and the concentration of salicylic acid is 20 mg / L.

[0064] Comparative Example 3

[0065] A composite plant growth regulator comprises the following main ingredients: cyanidin, triacontanol, uniconazole and salicylic acid.

[0066] The concentration of cyanidin is 25 mg / L; the concentration of triacontanol is 0.3 mg / L; the concentration of uniconazole is 40 mg / L; and the concentration of salicylic acid is 20 mg / L.

[0067] Compared with Example 2, this comparative example has the same raw materials and steps as Example 2 except that seabuckthorn oil is not added.

[0068] Comparative Example 4

[0069] A composite plant growth regulator comprises the following main ingredients: sea buckthorn oil, triacontanol, uniconazole and salicylic acid.

[0070] The concentration of seabuckthorn oil is 100 mg / L; the concentration of triacontanol is 0.3 mg / L; the concentration of uniconazole is 40 mg / L; and the concentration of salicylic acid is 20 mg / L.

[0071] Compared with Example 2, this comparative example is the same as Example 2 except that cyanidin is not added. The other raw materials and steps are the same as those of Example 2.

[0072] Performance Testing

[0073] Rice plant traits test

[0074] The experimental field selected a perennial rice-wheat rotation field with flat terrain and good fertility. The rice variety planted was Shengdao 18, the cultivation method was machine transplanting, and the field management was consistent with the local field. Set up the plant growth regulators of Examples 1-3 and Comparative Examples 1-4 and blank control test groups, a total of 8 groups, each with an area of ​​2 mu. The plant growth regulator application device was a drone, and the leaves were sprayed once at the tillering stage, jointing stage, and full heading stage of rice, respectively, with 30 liters per mu. In addition, each test group was applied with 98% potassium dihydrogen phosphate 30 g / mu at the tillering stage and full heading stage of rice. 30 days after the first application of the growth regulator, the rice seedlings were investigated, and 5 fixed sampling points were taken, 20 clumps were investigated at each point, and the number of tillers was recorded; the rice stem thickness, wall thickness, and chlorophyll SPAD value were measured two weeks after the rice plants reached full heading; the measured data are shown in Table 1. One month later, the rice plant height, panicle length, and the lengths of the first internode at the base, the second internode, and the internode below the panicle were measured and are shown in Table 2.

[0075] Table 1 Effects of plant growth regulators on plant traits

[0076]

[0077] As can be seen from Table 1, the composite plant growth regulator prepared by the present invention can promote rice tillering, and the stem thickness and wall thickness of rice are significantly improved after using the plant growth regulator of the present invention. In addition, the chlorophyll content is higher than that of the blank control. It can be seen that the composite plant growth regulator of the present invention can improve the photosynthesis of rice and promote the growth of the plant. In addition, the improvement of the stem thickness and wall thickness of rice helps to enhance the lodging resistance of rice.

[0078] Table 2 Effects of plant growth regulators on plant traits

[0079]

[0080] As shown in Table 2, compared with the blank control, the composite plant growth regulators of Examples 1-3 of the present invention all inhibited the height of rice plants and the length of the first and second internodes at the base, among which Example 2 had the most significant effect. The plant growth regulator of the present invention has a promoting effect on the internodes below the rice panicle and the panicle length. The improvement of these plant traits is conducive to enhancing the ability of rice to resist lodging. In addition, the composite plant growth regulator of the present invention can increase the panicle length of rice, thereby increasing the number of grains per panicle.

[0081] Rice yield experiment

[0082] At the rice maturity stage, each experimental group selected plants with similar growth in the central yield measurement area, measured the number of effective panicles within 1m×1m, and calculated the number of panicles per mu. At the same time, 6 holes of plants with similar growth were selected to calculate the number of grains per panicle and the thousand-grain weight. When the rice was harvested, each experimental group cut 5 m 2 Measure the yield, convert the actual yield, and compare the yield increase effect. The above measured data are shown in Table 3.

[0083] Table 3 Effects of plant growth regulators on rice yield

[0084]

[0085] The number of ears per mu, number of grains per ear, thousand-grain weight and actual yield of the plant growth regulator treatment groups of Examples 1-3 of the present invention are all higher than those of the blank control, indicating that the plant growth regulator of the present invention has a certain promoting effect on the growth and development of rice. Compared with the blank control, the composite plant growth regulator of the present invention has achieved increased yield. In addition, after changing the concentration of the substance used in the present invention or reducing the components, the number of ears per mu, number of grains per ear, thousand-grain weight and actual yield are all lower than those of the present invention, indicating that the plant growth regulator of the present invention has the best effect on the growth and development of rice within a specific sea buckthorn oil and brown cyanidin concentration range and after compounding with other components.

[0086] It should be noted that the above embodiments are only partial embodiments of the preferred methods of implementing the present invention, rather than all embodiments. Obviously, based on the above embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work should fall within the scope of protection of the present invention.

Claims

1. A composite plant growth regulator, characterized in that: Includes the following main ingredients: sea buckthorn oil, cyanidin, triacontanol, uniconazole, salicylic acid; The concentration of seabuckthorn oil is 50-200 mg / L, the concentration of cyanidin is 5-50 mg / L, the concentration of triacontanol is 0.2-0.4 mg / L, the concentration of uniconazole is 40-50 mg / L, and the concentration of salicylic acid is 10-30 mg / L; Wherein, the preparation method of seabuckthorn oil is: (1) The whole seabuckthorn fruit was dried in an oven at 40°C for 24 h, and then crushed at 4000 r / min for 3 s to obtain seabuckthorn fruit powder; the water content of the seabuckthorn fruit powder was adjusted to 20%, and then stirred evenly, and microwaved for 2 minutes to obtain a mixture A; (2) Add a composite hydrolase solution containing pectin lyase and Bacillus subtilis fibrinolytic enzyme to the mixture A, perform enzymatic hydrolysis at 40-50°C for 4 hours, centrifuge, and remove the lower precipitate; weigh the lower precipitate, add n-ethane, extract with stirring at 45°C for 1 hour, filter, and remove n-ethane from the filtrate by rotary evaporation and nitrogen blowing to obtain sea buckthorn oil; The composite plant growth regulator is used for increasing rice yield and resisting lodging.

2. The composite plant growth regulator according to claim 1, characterized in that: In step (1), the whole seabuckthorn fruit is a dried fruit; in step (2), the mass-volume ratio of the mixture A to the composite hydrolase solution is 1kg:1L, the composite hydrolase solution contains 18-24g / L of pectin lyase and 5-8g / L of Bacillus subtilis fibrinolytic enzyme, the enzyme activity of the pectin lyase is 400,000 U / g, and the enzyme activity of the Bacillus subtilis fibrinolytic enzyme is 100,000 U / g; in step (2), the weight-volume ratio of the lower layer precipitate to n-ethane is 1kg:8L.

3. The composite plant growth regulator according to claim 1, characterized in that: The concentration of seabuckthorn oil is 100 mg / L; the concentration of cyanidin is 25 mg / L; the concentration of triacontanol is 0.3 mg / L; the concentration of uniconazole is 40 mg / L; and the concentration of salicylic acid is 20 mg / L.

4. The composite plant growth regulator according to claim 1, characterized in that: The composite plant growth regulator is a liquid preparation.

5. A method for preparing the composite plant growth regulator according to any one of claims 1 to 4, characterized in that: Prepared by the following steps: (1) Take a dilution container, dissolve Tween-80 in 600 mL of hot water to obtain an emulsifier solution, weigh the mass of seabuckthorn oil according to the concentration used, add it to the emulsifier solution and stir until uniform to obtain a seabuckthorn oil emulsion, and cool it to room temperature for use; (2) Calculate the weight of cyanidin, triacontanol and uniconazole according to the concentration used, weigh cyanidin, triacontanol and uniconazole and dissolve them in ethanol, the total amount of ethanol does not exceed 50 ml / L after the solution is fixed to volume; add the dissolved cyanidin solution, triacontanol solution and uniconazole solution to the container of step (1) in sequence and stir evenly; (3) Finally, salicylic acid is weighed according to the concentration to be used, added into a container, stirred until dissolved, and then water is added to make up the volume to obtain a composite plant growth regulator.

6. The method for preparing the composite plant growth regulator according to claim 5, characterized in that: In the step (1), the mass ratio of seabuckthorn oil to Tween-80 is 1:

1.

7. Use of the composite plant growth regulator according to any one of claims 1 to 4 in increasing rice yield and resisting lodging.

8. The use according to claim 7, characterized in that: The composite plant growth regulator is used in the form of foliar spraying.

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