Fungal inhibitors containing oregano essential oil and methods for inhibiting fungi

Abstract

The present invention belongs to the technical field of fungal inhibitors, and specifically relates to a fungal inhibitor containing oregano essential oil and a method for inhibiting fungi. The fungal inhibitor is prepared by mixing the following components: 100 to 150 parts of oregano essential oil and 30 to 50 parts of thyme essential oil; the oregano essential oil is prepared by the following method: after the oregano whole herb is crushed, an extraction solvent is added, ultrasonic extraction is performed, and then steam distillation is performed, and the distillate is collected and mixed with olive oil to obtain the oregano essential oil; the thyme whole herb essential oil is prepared by the following method: after the thyme whole herb is crushed, olive oil is added, sealed and illuminated, and the collected liquid is the thyme whole herb essential oil. The fungal inhibitor containing oregano essential oil of the present invention can simultaneously inhibit Aspergillus flavus, Penicillium, Aspergillus and ergot.

Description

Technical Field

[0001] The invention belongs to the technical field of fungus inhibitors, and particularly relates to a fungus inhibitor containing oregano essential oil and a method for inhibiting fungi. Background Art

[0002] Mycotoxins are one of the common toxins found in food and medicinal materials. For example, Chinese medicinal materials are easily contaminated by fungi such as Aspergillus flavus during transportation and storage. After contamination, mycotoxins will be produced, affecting the quality and efficacy of Chinese medicinal materials.

[0003] Mycotoxins include aflatoxins, ochratoxins, ergot alkaloids, etc. Aflatoxins are produced by Aspergillus flavus, ochratoxins are mainly produced by Penicillium and Aspergillus, and ergot alkaloids are mainly produced by Claviceps ergotis. Among them, aflatoxins B1, B2, G1, G2, etc. produced by Aspergillus flavus are highly toxic and have a great impact on the quality and efficacy of Chinese medicinal materials.

[0004] There are many studies on the inhibition of fungal toxins in the prior art. For example, Chinese patent CN105028948A developed a mildew inhibitor for inhibiting aflatoxin, and Chinese patent CN108850003A developed a peanut mildew inhibitor and mildew prevention method for inhibiting the growth of Aspergillus flavus and inhibiting the aflatoxin content of peanuts. These prior arts can only inhibit a certain type or a certain class of fungi in a targeted manner, and have poor broad-spectrum properties.

[0005] Although there are oregano essential oil products in the prior art, their fungus-inhibiting effects are poor. Summary of the invention

[0006] In order to solve the above-mentioned technical problem that "the existing technology can only specifically inhibit a certain type or a certain class of fungi, and has poor broad-spectrum", and also to solve the problem that the existing oregano essential oil products have poor fungi inhibition effect, the present invention provides a fungi inhibitor containing oregano essential oil and a fungi inhibition method. The fungi inhibitor containing oregano essential oil of the present invention can simultaneously inhibit Aspergillus flavus, Penicillium, Aspergillus and ergot.

[0007] The object of the present invention is to provide a fungus inhibitor containing oregano essential oil, which is prepared by mixing the following components: 100 to 150 parts of oregano essential oil and 30 to 50 parts of thyme whole herb essential oil;

[0008] Described oregano essential oil is prepared according to the following method:

[0009] The whole oregano plant is crushed, an extraction solvent is added, ultrasonic extraction is performed, and then steam distillation is performed, and the distillate is collected and mixed with olive oil to obtain oregano essential oil.

[0010] The whole thyme essential oil is prepared according to the following method: the whole thyme is crushed, olive oil is added, sealed and left to stand under light for 14 to 21 days, filtered, and the collected liquid is the whole thyme essential oil;

[0011] The fungus includes at least one of Aspergillus flavus, Penicillium, Aspergillus and Claviceps.

[0012] Preferably, the fungal inhibitor containing oregano essential oil is prepared by crushing the whole oregano herb into a material with a particle size of 1 mm to 5 mm.

[0013] Preferably, the extraction solvent of the fungal inhibitor containing oregano essential oil is water, and the material-liquid ratio of the crushed whole oregano herb to the extraction solvent is 1:8-10.

[0014] Preferably, the ultrasonic extraction power of the fungal inhibitor containing oregano essential oil is 400 W and the extraction time is 30 min.

[0015] Preferably, in the above-mentioned fungus inhibitor containing oregano essential oil, when preparing the oregano essential oil, the volume ratio of the distillate to the olive oil is 1:1-2.

[0016] Preferably, the fungal inhibitor containing oregano essential oil is made by crushing the whole thyme into a material with a particle size of 1 mm to 5 mm.

[0017] Preferably, in the fungus inhibitor containing oregano essential oil, the material-liquid ratio of the crushed whole thyme herb to the olive oil is 1:10-12.

[0018] Preferably, the fungus inhibitor containing oregano essential oil has a light intensity of 4000 Lux to 6000 Lux.

[0019] Preferably, the fungal inhibitor containing oregano essential oil is used at a concentration of 0.2 μL / mL to 0.8 μL / mL.

[0020] Preferably, the method for inhibiting fungi using a fungal inhibitor containing oregano essential oil comprises at least one of the following:

[0021] The fungal inhibitor containing oregano essential oil is mixed with a fungal liquid to achieve a fungal inhibition effect;

[0022] Alternatively, fungal inhibitors containing oregano essential oil can be sprayed onto fungus-contaminated samples to achieve a fungal inhibitory effect;

[0023] Alternatively, fungus-contaminated samples can be placed over a fungus inhibitor containing oregano essential oil to inhibit the fungus through fumigation.

[0024] Compared with the prior art, the present invention has the following beneficial effects:

[0025] The present invention adopts a method combining ultrasonic extraction and steam distillation to extract the active ingredients of the whole oregano plant, and then mixes with olive oil to obtain oily oregano essential oil. The oiliness of olive oil is utilized to make the product oily. The present invention also uses olive oil as an extraction solvent to extract the active ingredients in the whole thyme plant, and combines the means of sealing and illumination to finally obtain the whole thyme plant essential oil. The oregano essential oil prepared by the present invention has the effect of inhibiting aflatoxin, and the whole thyme plant essential oil has the effect of inhibiting penicillium and aspergillus. Therefore, after the oregano essential oil and the whole thyme plant essential oil are compounded, the oregano essential oil occupies the main component, can simultaneously inhibit aspergillus flavus, penicillium, aspergillus and ergot, and has a good inhibitory effect on aflatoxin, ochratoxin and ergot alkali.

[0026] According to our experimental results, the compounding of oregano essential oil and thyme essential oil has significantly improved the fungi inhibition and mycotoxin inhibition effects compared with single factors, and increased the types of fungi inhibition and mycotoxin inhibition. And through experimental comparison, the fungi inhibitor containing oregano essential oil of the present invention has better antibacterial effect and mycotoxin inhibition effect than commercially available essential oil products. BRIEF DESCRIPTION OF THE DRAWINGS

[0027] Figure 1 The antibacterial effect of the dilution prepared with the fungus inhibitor containing oregano essential oil in Example 1 on Aspergillus flavus.

[0028] Figure 2 The results of the antibacterial experiment of Aspergillus flavus pellets cultured in liquid culture medium are shown in Figure 1. The A row shows the overall growth of the culture bottle, and the B row shows the partial enlarged picture of the culture bottle corresponding to the A row.

[0029] Figure 3 yes Figure 2 The test results of mycelium dry weight after the experiment. DETAILED DESCRIPTION

[0030] In order to enable those skilled in the art to better understand and implement the technical solution of the present invention, the present invention is further described below in conjunction with specific embodiments and drawings.

[0031] In the description of the present invention, unless otherwise specified, all reagents used are commercially available and all methods used are conventional techniques in the art.

[0032] Although there are many studies on inhibiting mycotoxins in the prior art, such as Chinese patent CN105028948A developed a mildew inhibitor for inhibiting aflatoxin, and Chinese patent CN108850003A developed a peanut mildew inhibitor and mildew prevention method, which are used to inhibit the growth of Aspergillus flavus and inhibit the aflatoxin content of peanuts, the prior art has not found a product that inhibits aflatoxin, ochratoxin, and ergot alkaloids at the same time.

[0033] Based on the above reasons, the present invention provides a fungus inhibitor containing oregano essential oil, which is prepared by mixing the following components: 100 to 150 parts of oregano essential oil and 30 to 50 parts of thyme essential oil.

[0034] The oregano essential oil is prepared according to the following method: the whole oregano herb is crushed, an extraction solvent is added, ultrasonic extraction is performed, and then steam distillation is performed, and the distillate is collected and mixed with olive oil to obtain the oregano essential oil.

[0035] The whole thyme essential oil is prepared according to the following method: the whole thyme is crushed, olive oil is added, sealed and left to stand under light for 14 to 21 days, filtered, and the collected liquid is the whole thyme essential oil.

[0036] In the scheme of the present invention, oregano essential oil has the effect of inhibiting Aspergillus flavus, thyme whole herb essential oil has the effect of inhibiting Penicillium and Aspergillus, and after oregano essential oil and thyme whole herb essential oil are compounded, Aspergillus flavus, Penicillium, Aspergillus and ergot can be inhibited at the same time, and aflatoxin, ochratoxin and ergot alkali are all had good inhibitory effect. In the present invention, after oregano essential oil and thyme whole herb essential oil are compounded, the effect of inhibiting fungi and inhibiting mycotoxins is significantly improved compared with the single factor, and the types of inhibiting fungi and inhibiting mycotoxins are increased.

[0037] In addition, according to the inventive concept of the present invention, the whole herb of oregano is crushed and then an extraction solvent is added, ultrasonic extraction is performed, and then steam distillation is performed, and the distillate is collected and mixed with olive oil to obtain oregano essential oil; the whole herb of oregano, that is, the whole plant of oregano is used, ultrasonic extraction is combined with steam distillation to fully extract the essential oil components of the whole herb of oregano, and finally mixed with olive oil to achieve a good oily texture, which is easy to form a film on the surface of the sample, thereby improving the effect of inhibiting fungi and inhibiting the production of fungal toxins.

[0038] According to the invention, the whole thyme is crushed, olive oil is added, sealed and illuminated, and then left to stand for 14 to 21 days, filtered, and the collected liquid is the whole thyme essential oil. In view of the physiological and biochemical characteristics of the whole thyme, olive oil is directly used for refining and extraction, the method is simple, and because it is extracted at room temperature, the structure of the active ingredients is not destroyed, thereby improving the effect of inhibiting fungi and the effect of inhibiting the production of fungal toxins.

[0039] Through the experimental comparison of the present invention, the oregano essential oil and the whole thyme essential oil have better antibacterial effect and fungal toxin inhibition effect than the essential oil products on the market.

[0040] Furthermore, the whole oregano herb is crushed into a material with a particle size of 1 mm to 5 mm. The oregano essential oil extracted from the material with this particle size has more active ingredients and better effects of inhibiting fungi and inhibiting the production of fungal toxins.

[0041] Furthermore, the extraction solvent is water, and the material-liquid ratio of the crushed oregano whole herb to the extraction solvent is 1:8 to 10. Water is used as the extraction solvent, which is healthy, environmentally friendly, and inexpensive.

[0042] Furthermore, the power of ultrasonic extraction is 400 W and the time is 30 min. The ultrasonic extraction conditions can accelerate the distillation of active ingredients.

[0043] Furthermore, when preparing oregano essential oil, the volume ratio of distillate to olive oil is 1:1 to 2. This volume ratio has a good mixing effect.

[0044] Furthermore, the whole thyme herb is crushed into a material with a particle size of 1 mm to 5 mm. The whole thyme herb essential oil extracted from the material with this particle size has more active ingredients.

[0045] Furthermore, the material-liquid ratio of the crushed whole thyme to the olive oil is 1:10-12, so that more active ingredients can be extracted.

[0046] Furthermore, the light intensity is 4000 Lux to 6000 Lux. The light intensity is moderate.

[0047] Furthermore, the use concentration of the fungal inhibitor containing oregano essential oil is 0.1 μL / mL to 0.3 μL / mL. Under these reasonable use concentrations, a good fungal inhibition effect can be achieved, and the fungal toxin content can be effectively reduced, which can save the use of oregano essential oil and thyme essential oil, and save energy and protect the environment.

[0048] Based on the same inventive concept, the present invention also provides a method for inhibiting fungi using a fungus inhibitor containing oregano essential oil, comprising: mixing the fungus inhibitor containing oregano essential oil with a fungal liquid to achieve an antibacterial effect; or spraying the fungus inhibitor containing oregano essential oil into a sample contaminated with fungi to achieve an antibacterial effect; or placing the sample contaminated with fungi above the fungus inhibitor containing oregano essential oil to inhibit fungi by fumigation. These methods for inhibiting fungi are simple to operate and have good effects.

[0049] Some embodiments are described in detail below.

[0050] 1. Examples of different formulations.

[0051] Example 1

[0052] A fungus inhibitor containing oregano essential oil is prepared by mixing the following components: 100 parts of oregano essential oil and 30 parts of thyme whole herb essential oil.

[0053] Described oregano essential oil is prepared according to the following method:

[0054] The whole herb of oregano is crushed and then an extraction solvent is added, ultrasonic extraction is performed, and then steam distillation is performed, and the distillate is collected and mixed with olive oil to obtain oregano essential oil; wherein the whole herb of oregano is crushed into a material with a particle size of 1 mm; the extraction solvent is water, and the material-liquid ratio of the material after the whole herb of oregano is crushed to the extraction solvent is 1kg:8L; the power of ultrasonic extraction is 400W, and the time is 30min; the volume ratio of the distillate to the olive oil is 1:1.

[0055] The whole thyme essential oil is prepared according to the following method: the whole thyme is crushed, olive oil is added, the mixture is sealed and left to stand under light for 14 days, and the mixture is filtered through a filter with a pore diameter of 0.5 mm, the filter residue is removed, and the collected liquid is the whole thyme essential oil; wherein the whole thyme is crushed into a material with a particle size of 1 mm; the material-liquid ratio of the crushed whole thyme to olive oil is 1 kg:10 L; and the light intensity is 4000 Lux.

[0056] Example 2

[0057] A fungus inhibitor containing oregano essential oil is prepared by mixing the following components: 120 parts of oregano essential oil and 30 parts of thyme whole herb essential oil.

[0058] Described oregano essential oil is prepared according to the following method:

[0059] The whole herb of oregano is crushed and then an extraction solvent is added, ultrasonic extraction is performed, and then steam distillation is performed, and the distillate is collected and mixed with olive oil to obtain oregano essential oil; wherein the whole herb of oregano is crushed into a material with a particle size of 1 mm; the extraction solvent is water, and the material-liquid ratio of the material after the whole herb of oregano is crushed to the extraction solvent is 1kg:8L; the power of ultrasonic extraction is 400W, and the time is 30min; the volume ratio of the distillate to the olive oil is 1:1.

[0060] The whole thyme essential oil is prepared according to the following method: the whole thyme is crushed, olive oil is added, the mixture is sealed and left to stand under light for 14 days, and the mixture is filtered through a filter with a pore diameter of 0.5 mm, the filter residue is removed, and the collected liquid is the whole thyme essential oil; wherein the whole thyme is crushed into a material with a particle size of 1 mm; the material-liquid ratio of the crushed whole thyme to olive oil is 1 kg:10 L; and the light intensity is 4000 Lux.

[0061] Example 3

[0062] A fungus inhibitor containing oregano essential oil is prepared by mixing the following components: 150 parts of oregano essential oil and 30 parts of thyme whole herb essential oil.

[0063] Described oregano essential oil is prepared according to the following method:

[0064] The whole herb of oregano is crushed and then an extraction solvent is added, ultrasonic extraction is performed, and then steam distillation is performed, and the distillate is collected and mixed with olive oil to obtain oregano essential oil; wherein the whole herb of oregano is crushed into a material with a particle size of 1 mm; the extraction solvent is water, and the material-liquid ratio of the material after the whole herb of oregano is crushed to the extraction solvent is 1kg:8L; the power of ultrasonic extraction is 400W, and the time is 30min; the volume ratio of the distillate to the olive oil is 1:1.

[0065] The whole thyme essential oil is prepared according to the following method: the whole thyme is crushed, olive oil is added, the mixture is sealed and left to stand under light for 14 days, and the mixture is filtered through a filter with a pore diameter of 0.5 mm, the filter residue is removed, and the collected liquid is the whole thyme essential oil; wherein the whole thyme is crushed into a material with a particle size of 1 mm; the material-liquid ratio of the crushed whole thyme to olive oil is 1 kg:10 L; and the light intensity is 4000 Lux.

[0066] Example 4

[0067] A fungus inhibitor containing oregano essential oil is prepared by mixing the following components: 100 parts of oregano essential oil and 40 parts of thyme whole herb essential oil.

[0068] Described oregano essential oil is prepared according to the following method:

[0069] The whole herb of oregano is crushed and then an extraction solvent is added, ultrasonic extraction is performed, and then steam distillation is performed, and the distillate is collected and mixed with olive oil to obtain oregano essential oil; wherein the whole herb of oregano is crushed into a material with a particle size of 1 mm; the extraction solvent is water, and the material-liquid ratio of the material after the whole herb of oregano is crushed to the extraction solvent is 1kg:8L; the power of ultrasonic extraction is 400W, and the time is 30min; the volume ratio of the distillate to the olive oil is 1:1.

[0070] The whole thyme essential oil is prepared according to the following method: the whole thyme is crushed, olive oil is added, the mixture is sealed and left to stand under light for 14 days, and the mixture is filtered through a filter with a pore diameter of 0.5 mm, the filter residue is removed, and the collected liquid is the whole thyme essential oil; wherein the whole thyme is crushed into a material with a particle size of 1 mm; the material-liquid ratio of the crushed whole thyme to olive oil is 1 kg:10 L; and the light intensity is 4000 Lux.

[0071] Example 5

[0072] A fungus inhibitor containing oregano essential oil is prepared by mixing the following components: 100 parts of oregano essential oil and 50 parts of thyme whole herb essential oil.

[0073] Described oregano essential oil is prepared according to the following method:

[0074] The whole herb of oregano is crushed and then an extraction solvent is added, ultrasonic extraction is performed, and then steam distillation is performed, and the distillate is collected and mixed with olive oil to obtain oregano essential oil; wherein the whole herb of oregano is crushed into a material with a particle size of 1 mm; the extraction solvent is water, and the material-liquid ratio of the material after the whole herb of oregano is crushed to the extraction solvent is 1kg:8L; the power of ultrasonic extraction is 400W, and the time is 30min; the volume ratio of the distillate to the olive oil is 1:1.

[0075] The whole thyme essential oil is prepared according to the following method: the whole thyme is crushed, olive oil is added, the mixture is sealed and left to stand under light for 14 days, and the mixture is filtered through a filter with a pore diameter of 0.5 mm, the filter residue is removed, and the collected liquid is the whole thyme essential oil; wherein the whole thyme is crushed into a material with a particle size of 1 mm; the material-liquid ratio of the crushed whole thyme to olive oil is 1 kg:10 L; and the light intensity is 4000 Lux.

[0076] 2. Examples of different methods for preparing oregano essential oils.

[0077] Example 6

[0078] A fungus inhibitor containing oregano essential oil is prepared by mixing the following components: 100 parts of oregano essential oil and 30 parts of thyme whole herb essential oil.

[0079] Described oregano essential oil is prepared according to the following method:

[0080] The whole oregano herb is crushed and an extraction solvent is added thereto, ultrasonic extraction is performed, and then steam distillation is performed. The distillate is collected and mixed with olive oil to obtain oregano essential oil; wherein the whole oregano herb is crushed into a material with a particle size of 5 mm; the extraction solvent is water, and the material-liquid ratio of the material after the whole oregano herb is crushed to the extraction solvent is 1kg:8L; the power of ultrasonic extraction is 400W, and the time is 30min; and the volume ratio of the distillate to the olive oil is 1:1.

[0081] The whole thyme essential oil is prepared according to the following method: the whole thyme is crushed, olive oil is added, the mixture is sealed and left to stand under light for 14 days, and the mixture is filtered through a filter with a pore diameter of 0.5 mm, the filter residue is removed, and the collected liquid is the whole thyme essential oil; wherein the whole thyme is crushed into a material with a particle size of 1 mm; the material-liquid ratio of the crushed whole thyme to olive oil is 1 kg:10 L; and the light intensity is 4000 Lux.

[0082] Example 7

[0083] A fungus inhibitor containing oregano essential oil is prepared by mixing the following components: 100 parts of oregano essential oil and 30 parts of thyme whole herb essential oil.

[0084] Described oregano essential oil is prepared according to the following method:

[0085] The whole oregano herb is crushed and an extraction solvent is added thereto, ultrasonic extraction is performed, and then steam distillation is performed. The distillate is collected and mixed with olive oil to obtain oregano essential oil; wherein the whole oregano herb is crushed into a material with a particle size of 1 mm; the extraction solvent is water, and the material-liquid ratio of the material after the whole oregano herb is crushed to the extraction solvent is 1kg:10L; the power of ultrasonic extraction is 400W, and the time is 30min; the volume ratio of the distillate to the olive oil is 1:1.

[0086] The whole thyme essential oil is prepared according to the following method: the whole thyme is crushed, olive oil is added, the mixture is sealed and left to stand under light for 14 days, and the mixture is filtered through a filter with a pore diameter of 0.5 mm, the filter residue is removed, and the collected liquid is the whole thyme essential oil; wherein the whole thyme is crushed into a material with a particle size of 1 mm; the material-liquid ratio of the crushed whole thyme to olive oil is 1 kg:10 L; and the light intensity is 4000 Lux.

[0087] Example 8

[0088] A fungus inhibitor containing oregano essential oil is prepared by mixing the following components: 100 parts of oregano essential oil and 30 parts of thyme whole herb essential oil.

[0089] Described oregano essential oil is prepared according to the following method:

[0090] The whole herb of oregano is crushed and an extraction solvent is added thereto, ultrasonic extraction is performed, and then steam distillation is performed. The distillate is collected and mixed with olive oil to obtain oregano essential oil; wherein the whole herb of oregano is crushed into a material with a particle size of 1 mm; the extraction solvent is water, and the material-liquid ratio of the material after the whole herb of oregano is crushed to the extraction solvent is 1kg:8L; the power of ultrasonic extraction is 400W, and the time is 30min; the volume ratio of the distillate to the olive oil is 1:2.

[0091] The whole thyme essential oil is prepared according to the following method: the whole thyme is crushed, olive oil is added, the mixture is sealed and left to stand under light for 14 days, and the mixture is filtered through a filter with a pore diameter of 0.5 mm, the filter residue is removed, and the collected liquid is the whole thyme essential oil; wherein the whole thyme is crushed into a material with a particle size of 1 mm; the material-liquid ratio of the crushed whole thyme to olive oil is 1 kg:10 L; and the light intensity is 4000 Lux.

[0092] 3. Embodiments of different methods for preparing whole thyme essential oils

[0093] Example 9

[0094] A fungus inhibitor containing oregano essential oil is prepared by mixing the following components: 100 parts of oregano essential oil and 30 parts of thyme whole herb essential oil.

[0095] Described oregano essential oil is prepared according to the following method:

[0096] The whole herb of oregano is crushed and then an extraction solvent is added, ultrasonic extraction is performed, and then steam distillation is performed, and the distillate is collected and mixed with olive oil to obtain oregano essential oil; wherein the whole herb of oregano is crushed into a material with a particle size of 1 mm; the extraction solvent is water, and the material-liquid ratio of the material after the whole herb of oregano is crushed to the extraction solvent is 1kg:8L; the power of ultrasonic extraction is 400W, and the time is 30min; the volume ratio of the distillate to the olive oil is 1:1.

[0097] The whole thyme essential oil is prepared according to the following method: the whole thyme is crushed, olive oil is added, the mixture is sealed and left to stand under light for 21 days, and the mixture is filtered through a filter with a pore diameter of 0.5 mm, the residue is removed, and the collected liquid is the whole thyme essential oil; wherein the whole thyme is crushed into a material with a particle size of 1 mm; the material-liquid ratio of the crushed whole thyme to olive oil is 1 kg:10 L; and the light intensity is 4000 Lux.

[0098] Example 10

[0099] A fungus inhibitor containing oregano essential oil is prepared by mixing the following components: 100 parts of oregano essential oil and 30 parts of thyme whole herb essential oil.

[0100] Described oregano essential oil is prepared according to the following method:

[0101] The whole herb of oregano is crushed and then an extraction solvent is added, ultrasonic extraction is performed, and then steam distillation is performed, and the distillate is collected and mixed with olive oil to obtain oregano essential oil; wherein the whole herb of oregano is crushed into a material with a particle size of 1 mm; the extraction solvent is water, and the material-liquid ratio of the material after the whole herb of oregano is crushed to the extraction solvent is 1kg:8L; the power of ultrasonic extraction is 400W, and the time is 30min; the volume ratio of the distillate to the olive oil is 1:1.

[0102] The whole thyme essential oil is prepared according to the following method: the whole thyme is crushed, olive oil is added, the mixture is sealed and left to stand under light for 14 days, and the mixture is filtered through a filter with a pore diameter of 0.5 mm, the residue is removed, and the collected liquid is the whole thyme essential oil; wherein the whole thyme is crushed into a material with a particle size of 5 mm; the material-liquid ratio of the crushed whole thyme to olive oil is 1 kg:10 L; and the light intensity is 4000 Lux.

[0103] Embodiment 11

[0104] A fungus inhibitor containing oregano essential oil is prepared by mixing the following components: 100 parts of oregano essential oil and 30 parts of thyme whole herb essential oil.

[0105] Described oregano essential oil is prepared according to the following method:

[0106] The whole herb of oregano is crushed and then an extraction solvent is added, ultrasonic extraction is performed, and then steam distillation is performed, and the distillate is collected and mixed with olive oil to obtain oregano essential oil; wherein the whole herb of oregano is crushed into a material with a particle size of 1 mm; the extraction solvent is water, and the material-liquid ratio of the material after the whole herb of oregano is crushed to the extraction solvent is 1kg:8L; the power of ultrasonic extraction is 400W, and the time is 30min; the volume ratio of the distillate to the olive oil is 1:1.

[0107] The whole thyme essential oil is prepared according to the following method: the whole thyme is crushed, olive oil is added, the mixture is sealed and left to stand under light for 14 days, and the mixture is filtered through a filter with a pore diameter of 0.5 mm, the residue is removed, and the collected liquid is the whole thyme essential oil; wherein the whole thyme is crushed into a material with a particle size of 1 mm; the material-liquid ratio of the crushed whole thyme to olive oil is 1 kg:12 L; and the light intensity is 4000 Lux.

[0108] Example 12

[0109] A fungus inhibitor containing oregano essential oil is prepared by mixing the following ingredients: 100 parts of oregano essential oil and 30 parts of thyme whole herb essence.

[0110] Described oregano essential oil is prepared according to the following method:

[0111] The whole herb of oregano is crushed and then an extraction solvent is added, ultrasonic extraction is performed, and then steam distillation is performed, and the distillate is collected and mixed with olive oil to obtain oregano essential oil; wherein the whole herb of oregano is crushed into a material with a particle size of 1 mm; the extraction solvent is water, and the material-liquid ratio of the material after the whole herb of oregano is crushed to the extraction solvent is 1kg:8L; the power of ultrasonic extraction is 400W, and the time is 30min; the volume ratio of the distillate to the olive oil is 1:1.

[0112] The whole thyme essential oil is prepared according to the following method: the whole thyme is crushed, olive oil is added, the mixture is sealed and left to stand under light for 14 days, and the mixture is filtered through a filter with a pore diameter of 0.5 mm, the filter residue is removed, and the collected liquid is the whole thyme essential oil; wherein the whole thyme is crushed into a material with a particle size of 1 mm; the material-liquid ratio of the crushed whole thyme to olive oil is 1 kg:10 L; and the light intensity is 6000 Lux.

[0113] 4. Experiments on inhibiting fungi.

[0114] Experimental strains: Aspergillus flavus ATCC26768, Penicillium spp. ATCC10428, Aspergillus niger ATCC1036 and E. coli ATCCBAA-1454.

[0115] Experimental method: (1) Prepare spores with a concentration of 10 6 CFU / mL of Aspergillus flavus suspension, Penicillium spp. suspension, Aspergillus niger suspension, and Claviceps ergot suspension.

[0116] (2) Using 0.01% by volume Tween-80 as a solvent, the fungal inhibitors containing oregano essential oil from different embodiments were diluted to a concentration of 0.2 μL / mL, 0.4 μL / mL, 0.6 μL / mL, and 0.8 μL / mL, respectively.

[0117] (3) Take 100 μL of the dilution and spread it evenly on the surface of high-salt Czapek agar (COA) medium. Spread an equal volume of 0.01% Tween-80 on the control group (ck). Add 5 μL of fungal liquid on a 6 mm sterile filter paper and place it in the center of COA. Culture at 28±1℃ for 5 days. After the end of the 5th day, measure the colony diameter using the cross method. Repeat three times and take the average diameter.

[0118] The evaluation criteria for antibacterial effect are as follows:

[0119] A colony diameter of less than 7 mm is highly sensitive to inhibition, 7 to 9 mm is moderately sensitive to inhibition, between 10 and 20 mm is lowly sensitive to inhibition, and greater than 20 mm is no inhibitory effect.

[0120] The other antibacterial effects are shown in Table 1. Figure 1 The antifungal effect of the dilution prepared with the fungal inhibitor containing oregano essential oil in Example 1 on Aspergillus flavus was exemplified. The results showed that the dilution with a concentration of 0.2 μL / mL to 0.8 μL / mL had a good antifungal effect. Figure 2 The Aspergillus flavus pellets cultured in liquid culture medium demonstrate the antibacterial effect of the dilution prepared with the fungal inhibitor containing oregano essential oil of Example 1 on Aspergillus flavus. Figure 3 yes Figure 2 The test results of mycelium dry weight after the experiment. In addition, we also tested the total amount of aflatoxin AFB1, AFB2, AFG1 and AFG2, the total amount of ochratoxin, and the total amount of ergot alkaloids in the culture medium, all of which were at least reduced to below 35μg / kg. In the experiment of 0.8μL / mL dilution, since there was no mycelium growth, the content of the above-mentioned mycotoxins was 0.

[0121] Table 1 Antibacterial effect

[0122]

[0123]

[0124]

[0125] (4) The fumigation method was used to evaluate the inhibitory effect of essential oils on the growth of Aspergillus flavus.

[0126] Pipette 50μL of bacterial suspension and spread it evenly on COA. After the bacterial suspension is absorbed by agar, turn it upside down and let it stand. Add 5μL of essential oils of different concentrations on a 6mm sterile filter paper and place it in the center of the inner side of the culture dish cover. Add an equal volume of sterile water to the control group. Culture at 28±1℃ for 7 days. Then test the fungal colony diameter. Refer to the following formula for inhibition evaluation:

[0127]

[0128] Note: W, mycelium growth inhibition rate; D C , fungal colony diameter of the control group; D T , fungal colony diameter in the experimental group.

[0129] The experimental results are shown in Table 2. The results in Table 2 show that the fungal inhibitors containing oregano essential oil provided in each embodiment have good antibacterial effects. The inhibition rate effect of the control group ck is because the fumigation method itself also has a certain antibacterial effect. It should be noted that in Table 2, since there is no mycelium growth in the experiment of 0.8 μL / mL dilution, the statistical mycelium growth inhibition rate is 100%.

[0130] Table 2 Mycelium growth inhibition rate results

[0131]

[0132]

[0133]

[0134] It should be noted that when the present invention involves a numerical range, it should be understood that the two endpoints of each numerical range and any value between the two endpoints can be selected. Since the steps and methods used are the same as those in the embodiments, in order to avoid redundancy, the present invention describes a preferred embodiment. Although the preferred embodiments of the present invention have been described, once those skilled in the art know the basic inventive concept, they can make other changes and modifications to these embodiments, and these changes and modifications all fall within the scope of the present invention.

[0135] Obviously, those skilled in the art can make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. If these modifications and variations of the present invention fall within the scope of the equivalent technology of the present invention, the present invention is also intended to include these modifications and variations.

Claims

1. A use of a fungus inhibitor containing oregano essential oil in fungus inhibition, characterized in that: The fungal inhibitor containing oregano essential oil is prepared by mixing the following ingredients: 100 to 150 parts of oregano essential oil and 30 to 50 parts of thyme essential oil; Described oregano essential oil is prepared according to the following method: The whole oregano plant is crushed, an extraction solvent is added, ultrasonic extraction is performed, and then steam distillation is performed, and the distillate is collected and mixed with olive oil to obtain oregano essential oil. The whole thyme essential oil is prepared according to the following method: the whole thyme is crushed, olive oil is added, sealed, and left to stand under light for 14 to 21 days, filtered, and the collected liquid is the whole thyme essential oil; The fungus is ergot.

2. The use of the fungus inhibitor containing oregano essential oil according to claim 1 in inhibiting fungi, characterized in that: The whole oregano herb is crushed into particles with a diameter of 1 mm to 5 mm.

3. The use of the fungus inhibitor containing oregano essential oil according to claim 2 in inhibiting fungi, characterized in that: The extraction solvent is water, and the material-liquid ratio of the crushed whole oregano herb to the extraction solvent is 1:8-10.

4. The use of the fungus inhibitor containing oregano essential oil according to claim 1 in inhibiting fungi, characterized in that: The power of ultrasonic extraction was 400 W and the time was 30 min.

5. The use of the fungus inhibitor containing oregano essential oil according to claim 1 in inhibiting fungi, characterized in that: When preparing oregano essential oil, the volume ratio of distillate to olive oil is 1:1~2.

6. Use of the fungus inhibitor containing oregano essential oil according to claim 1 in inhibiting fungi, characterized in that: The whole thyme herb is crushed into particles with a diameter of 1 mm to 5 mm.

7. Use of the fungus inhibitor containing oregano essential oil according to claim 6 in inhibiting fungi, characterized in that: The material-liquid ratio of the crushed whole thyme herb to olive oil is 1:10~12.

8. Use of the fungus inhibitor containing oregano essential oil in fungus inhibition according to claim 1, characterized in that: The light intensity is 4000Lux~6000Lux.

9. Use of the fungus inhibitor containing oregano essential oil according to claim 1 in inhibiting fungi, characterized in that: The concentration for inhibiting fungi is 0.2μL / mL~0.8μL / mL.

10. A method for inhibiting fungi, characterized in that: Include at least one of the following: Mixing the fungal inhibitor containing oregano essential oil according to claim 1 with a fungal liquid; spraying the fungus inhibitor containing oregano essential oil into the sample contaminated with fungi; placing the fungus-contaminated sample on top of the fungus inhibitor containing oregano essential oil for fumigation; Wherein, the fungus is ergot.

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