Needle for lavage in mouse peritoneal macrophage separation

By designing a shrinkable needle and a needle for cleaning the filter with a bristle, the problem of prone to piercing too deep and difficulty in absorbing the filter is solved, achieving more efficient abdominal fluid aspiration and cell separation.

CN119235495AInactive Publication Date: 2025-01-03SHANXI MEDICAL UNIV
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Patent Information

Application Number
CN202411574999.0
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2024-11-06
Publication Date
2025-01-03
Estimated Expiration
Not applicable · inactive patent

AI Technical Summary

Technical Problem

The sharp tip of traditional syringes can easily penetrate too deeply and damage the intestinal wall, resulting in increased contamination and difficulty in absorbing fluid, less liquid aspiration, and less cell separation efficiency.

Method used

A needle is designed that contains a retractable needle and filter mesh that controls the telescopic and limit of the needle through an adjustment knob to prevent too deep punctures and cleans the filter mesh with a water wheel-driven bristle to prevent tissue clogging.

Benefits of technology

Effectively prevent the needle from punctured too deeply, reduce contamination, improve the liquid absorption efficiency, ensure sufficient amount of liquid aspiration, and improve cell separation efficiency.

✦ Generated by Eureka AI based on patent content.

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Abstract

The invention discloses a needle head for abdominal cavity lavage in mouse peritoneal macrophage separation, and relates to the technical field of needle heads for abdominal cavity lavage, the needle head comprises a needle tube joint, one side of the needle tube joint is provided with a mounting port, a needle tube body is fixedly mounted in the mounting port, the surface of the needle tube body is provided with a suction port, and the suction port is provided with a suction port. By rotating an adjusting knob, the adjusting knob drives a wrapping shell to rotate, the wrapping shell drives a threaded guide tube to rotate, the threaded guide tube drives a needle head to be limited and guided by a hexagonal rod, and a sliding piece drives a hemispherical clamping block to move; the semispherical clamping block is inserted into the clamping groove to limit the adjusting knob, so that the wrapping shell and the needle head are limited, and the situation that in the follow-up operation process, the needle head shakes, so that the needle head pierces into the intestinal wall too deeply, the intestinal wall is damaged, pollution is caused, and an experiment fails is prevented.
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Description

Technical Field

[0001] The present invention relates to the technical field of needles for peritoneal lavage, and in particular to a needle for peritoneal lavage in mouse peritoneal macrophage separation. Background Art

[0002] Mouse peritoneal macrophages are a type of immune cell with multiple important functions. They are an important subject for studying phagocytosis, cellular immunity, and molecular immunology. When isolating mouse peritoneal macrophages, they need to be extracted first. Before extraction, the mouse's peritoneal cavity needs to be flushed with PBS after being killed. That is, PBS buffer is injected into the peritoneal cavity, the mouse's abdomen is massaged, and the intraperitoneal fluid is aspirated after a few minutes for extraction.

[0003] When performing peritoneal lavage, conventional methods often use conventional syringes to inject and aspirate fluid. However, the tip of a conventional syringe is sharp, and if it is accidentally inserted too deep, it can easily damage the intestinal wall, causing contamination and leading to experimental failure. When aspirating fluid, it often absorbs intra-abdominal tissue and blocks the needle, greatly increasing the difficulty of aspirating peritoneal fluid and reducing the amount of aspirated fluid, resulting in reduced cell separation efficiency. Therefore, we disclose a needle for peritoneal lavage of mouse peritoneal macrophage isolation to meet people's needs. Summary of the Invention

[0004] The purpose of the present application is to provide a needle for peritoneal lavage of mouse peritoneal macrophage isolation, so as to solve the problem that the tip of the conventional syringe proposed in the above background technology is sharp, and if it is accidentally inserted too deep, it is easy to damage the intestinal wall, causing contamination and leading to experimental failure. When sucking out the liquid, it often sucks up the intra-abdominal tissue and blocks the needle, which greatly increases the difficulty of sucking out the peritoneal fluid and also makes the amount of sucked out liquid less, resulting in a decrease in cell separation efficiency.

[0005] To achieve the above objectives, the present application provides the following technical solution: a needle for peritoneal lavage in mouse peritoneal macrophage isolation, comprising a needle-tube connector, a mounting opening being defined on one side of the needle-tube connector, a needle body being fixedly mounted within the mounting opening, a suction port being defined on the surface of the needle body, and a puncture assembly being rotatably mounted on the surface of the needle body for puncture;

[0006] The puncture assembly includes a wrapping shell, which is rotatably mounted on the surface of the needle tube connector. A connecting port corresponding to the suction port is opened on the surface of the wrapping shell. A hexagonal rod is fixedly mounted on one end of the needle tube body, and a needle for puncture is slidably mounted on the surface of the hexagonal rod. A threaded guide tube is fixedly mounted on one side of the wrapping shell, and the surface of the needle is threadedly mounted on the inner wall of the threaded guide tube. A filter screen is fixedly mounted in the suction port to prevent the needle tube body from being blocked, a positioning component is fixedly mounted on the surface of the wrapping shell to facilitate positioning, and an anti-blocking component is fixedly mounted on the inner wall of the needle tube body to prevent the filter screen from being blocked.

[0007] Preferably, the positioning assembly includes an adjusting knob, which is fixedly mounted on the surface of the wrapping shell, a mounting groove is provided on one side of the adjusting knob, a slide is slidably installed in the mounting groove, a hemispherical clamping block is fixedly installed on one side of the slide, a plurality of clamping grooves for facilitating clamping are provided on one side of the needle and tube connector, and a reset unit is fixedly mounted on the other side of the slide.

[0008] Preferably, the reset unit includes a telescopic rod, one end of which is fixedly mounted on one side of the slide, and the other end of which is fixedly mounted on the inner wall of the mounting groove, and a spring is sleeved on the surface of the telescopic rod.

[0009] Preferably, the anti-blocking assembly includes several connecting rods, one end of several connecting rods is fixedly mounted with the same mounting plate, the surface of the mounting plate is slidably mounted with a reciprocating block through several connecting rods, one side of the reciprocating block is fixedly mounted with a brush plate, the inner wall of the brush plate is fixedly mounted with several bristles for cleaning the filter, and the inner wall of the needle tube body is fixedly mounted with a power unit.

[0010] Preferably, the power unit includes a rotating rod, which is rotatably mounted on the inner wall of the needle tube body, a water wheel is fixedly mounted on the surface of the rotating rod, a rotating disk is fixedly mounted on one end of the rotating rod, a circular opening is provided on one side of the mounting plate to facilitate the movement of the rotating disk, a driving block is fixedly mounted on the surface of the rotating disk, a rectangular opening is provided on one side of the reciprocating block, and the driving block is slidably connected to the inner wall of the rectangular opening.

[0011] Preferably, an external rod is fixedly mounted on one side of the adjusting knob, a limiting rod is fixedly mounted on one side of the external rod, and two limiting blocks for limiting the limiting rod are fixedly mounted on the surface of the needle and tube connector.

[0012] Preferably, a plurality of extrusion blocks are fixedly mounted on the inner wall of the needle and tube connector for facilitating external connection.

[0013] Preferably, the surface of the threaded guide tube is designed to be inclined to fit the surface of the needle.

[0014] Preferably, the surface of the adjusting knob is provided with an anti-slip groove.

[0015] Preferably, the connecting rod is dumbbell-shaped.

[0016] In summary, the technical effects and advantages of the present invention are as follows:

[0017] 1. In the present invention, by rotating the adjusting knob, the adjusting knob drives the wrapping shell to rotate, the wrapping shell drives the threaded guide tube to rotate, and the threaded guide tube drives the needle under the limiting and guiding action of the hexagonal rod, so that the needle begins to retract, and at the same time the connecting port rotates together and gradually aligns with the suction port. When the needle is fully retracted, the connecting port is aligned with the suction port, and then the slide is driven by the spring to move under the action of the telescopic rod, and the slide drives the hemispherical clamping block to move, and the hemispherical clamping block is inserted into the clamping groove to complete the limiting of the adjusting knob, thereby completing the limiting of the wrapping shell and the needle, preventing the needle from shaking during subsequent operations, causing the needle to penetrate too deeply and injure the intestinal wall, causing contamination, and resulting in experimental failure.

[0018] 2. In the present invention, the filter provided in the suction port can effectively prevent the intra-abdominal tissue from directly entering the needle body when the PBS buffer is aspirated, thereby blocking the needle body and increasing the difficulty of aspirating the liquid.

[0019] 3. In the present invention, during the process of PBS buffer absorption, the PBS buffer flows through the water wheel, causing the water wheel to rotate, which drives the rotating rod to rotate, the rotating rod drives the rotating disk to rotate, the rotating disk drives the driving block to rotate, and the driving block drives the reciprocating block to reciprocate under the action of four connecting rods through the rectangular opening. The reciprocating block drives the brush plate to reciprocate, and the brush plate drives the bristles to reciprocate. The bristles are attached to one side of the filter screen, which can remove the abdominal cavity tissue contaminated on the filter screen to prevent it from clogging the filter screen and increasing the difficulty of liquid absorption. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the following briefly introduces the drawings required for use in the embodiments or the description of the prior art. Obviously, the drawings described below are only some embodiments of the present application. For those skilled in the art, other drawings can be obtained based on these drawings without paying any creative work.

[0021] Figure 1 It is a schematic diagram of the three-dimensional structure of the present invention;

[0022] Figure 2 is a cross-sectional view of the wrapping shell of the present invention;

[0023] Figure 3 for Figure 2 Schematic diagram of the local enlarged structure of area A in the middle;

[0024] Figure 4 is a cross-sectional view of the needle tube body of the present invention;

[0025] Figure 5 Schematic diagram of the cross-section structure of the mounting plate in the present invention;

[0026] Figure 6 Schematic diagram of the needle-tube connector and its related structures in the present invention.

[0027] In the figure: 1. needle and tube connector; 2. needle and tube body; 3. wrapping shell; 4. threaded guide tube; 5. hexagonal rod; 6. needle; 7. connecting port; 8. filter screen; 9. adjusting knob; 10. slide; 11. hemispherical clamping block; 12. clamping groove; 13. telescopic rod; 14. connecting rod; 15. mounting plate; 16. reciprocating block; 17. brush plate; 18. rotating rod; 19. bristles; 20. rotating disk; 21. driving block; 22. water wheel; 23. external rod; 24. limit rod; 25. limit block; 26. extrusion block; 27. rectangular opening; 28. suction port; 29. ​​spring. DETAILED DESCRIPTION

[0028] The following will clearly and completely describe the technical solutions in the embodiments of the present invention in conjunction with the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative efforts are within the scope of protection of the present invention.

[0029] See also Figures 1-6 , the embodiment provided by the present invention:

[0030] A needle for peritoneal lavage in mouse peritoneal macrophage isolation, comprising a needle-tube connector 1, a mounting opening formed on one side of the needle-tube connector 1, a needle body 2 fixedly mounted within the mounting opening, a suction port 28 formed on the surface of the needle body 2, and a puncture assembly rotatably mounted on the surface of the needle body 2 for puncture;

[0031] The puncture assembly includes a wrapping shell 3, which is rotatably mounted on the surface of the needle tube connector 1. A communication port 7 corresponding to the suction port 28 is opened on the surface of the wrapping shell 3. A hexagonal rod 5 is fixedly mounted on one end of the needle tube body 2. A needle 6 for puncture is slidably mounted on the surface of the hexagonal rod 5. A threaded guide tube 4 is fixedly mounted on one side of the wrapping shell 3. The surface of the needle 6 is screwed on the inner wall of the threaded guide tube 4. A filter screen 8 is fixedly mounted in the suction port 28 to prevent the needle tube body 2 from being blocked. A positioning component is fixedly mounted on the surface of the wrapping shell 3 to facilitate positioning. The inner wall of the needle tube body 2 An anti-blocking component is fixedly installed to prevent the filter 8 from being blocked. The end of the needle body 2 away from the needle connector 1 is sealed, and a suction port 28 is provided on its surface near the sealed end for injecting or aspirating PBS buffer. The size of the suction port 28 is the same as that of the connecting port 7, and a filter 8 is provided at the suction port 28. The filter holes of the filter 8 are arranged in a rectangular shape and are used to filter abdominal cavity tissue. The needle 6 is conical, and the surface of the needle 6 is provided with a threaded line that is screwed to the thread groove of the threaded guide tube 4. When the threaded guide tube 4 rotates, the needle 6 will be driven to retract or extend.

[0032] like Figure 2 As shown, the surface of the threaded guide tube 4 is designed to be inclined and fits the surface of the needle 6. The thickness of the threaded guide tube 4 gradually decreases on the side close to the needle 6 and fits the surface of the needle 6, making it easier to operate during puncture.

[0033] like Figure 2 and Figure 3 As shown, the positioning assembly includes an adjusting knob 9, which is fixedly mounted on the surface of the wrapping shell 3. A mounting groove is provided on one side of the adjusting knob 9, in which a slide 10 is slidably mounted. A hemispherical clamping block 11 is fixedly mounted on one side of the slide 10. A plurality of clamping grooves 12 for facilitating clamping are provided on one side of the needle and tube connector 1. A reset unit is fixedly mounted on the other side of the slide 10. An annular and equiangular clamping groove 12 is provided on one side of the needle and tube connector 1. The clamping groove 12 is spherical and adapted to the hemispherical clamping block 11.

[0034] like Figure 3As shown, the reset unit includes a telescopic rod 13, one end of the telescopic rod 13 is fixedly mounted on one side of the slide 10, and the other end of the telescopic rod 13 is fixedly mounted on the inner wall of the mounting groove. The surface of the telescopic rod 13 is sleeved with a spring 29. The adjusting knob 9 is turned, and the adjusting knob 9 drives the wrapping shell 3 to rotate. The wrapping shell 3 drives the threaded guide tube 4 to rotate. The threaded guide tube 4 drives the needle 6 under the limiting guidance of the hexagonal rod 5, so that the needle 6 begins to retract, and at the same time, the connecting port 7 rotates together and gradually aligns with the connecting port 7. The suction port 28 is aligned. When the needle 6 is fully retracted, the connecting port 7 is aligned with the suction port 28. The spring 29 drives the slide 10 to move under the action of the telescopic rod 13. The slide 10 drives the hemispherical clamping block 11 to move. The hemispherical clamping block 11 is inserted into the clamping groove 12 to complete the limiting of the adjusting knob 9, thereby completing the limiting of the wrapping shell 3 and the needle 6, preventing the needle 6 from shaking during subsequent operations, causing the needle 6 to penetrate too deeply and injure the intestinal wall, causing contamination, and leading to the failure of the experiment.

[0035] like Figure 4 and Figure 5 As shown, the anti-blocking component includes several connecting rods 14, one end of which is fixedly mounted with the same mounting plate 15, and the surface of the mounting plate 15 is slidably mounted with a reciprocating block 16 through several connecting rods 14, and a brush plate 17 is fixedly mounted on one side of the reciprocating block 16, and the inner wall of the brush plate 17 is fixedly mounted with several bristles 19 for cleaning the filter screen 8, and the inner wall of the needle tube body 2 is fixedly mounted with a power unit, and a total of four connecting rods 14 are provided, and the connecting rods 14 are dumbbell-shaped, and the reciprocating block 16 is slidably mounted on the surface of the four connecting rods 14, and the bristles 19 are made of soft silicone and perform reciprocating motion during the anti-blocking operation, so that the bristles 19 brush the rectangular filter holes to prevent them from being blocked.

[0036] like Figure 4 and Figure 5As shown, the power unit includes a rotating rod 18, which is rotatably mounted on the inner wall of the needle body 2, a water wheel 22 is fixedly mounted on the surface of the rotating rod 18, a rotating disk 20 is fixedly mounted on one end of the rotating rod 18, a circular opening is opened on one side of the mounting plate 15 to facilitate the movement of the rotating disk 20, a driving block 21 is fixedly mounted on the surface of the rotating disk 20, a rectangular opening 27 is opened on one side of the reciprocating block 16, the driving block 21 is slidably connected to the inner wall of the rectangular opening 27, the water wheel 22 is located in the central area inside the needle body 2, and when the PBS buffer flows through the water wheel 22, the water The wheel 22 rotates, and the driving block 21 is installed in the outer ring area of ​​the rotating disk 20. The water wheel 22 drives the rotating rod 18 to rotate, the rotating rod 18 drives the rotating disk 20 to rotate, and the rotating disk 20 drives the driving block 21 to rotate. The driving block 21 drives the reciprocating block 16 to reciprocate under the action of the four connecting rods 14 through the rectangular opening 27. The reciprocating block 16 drives the brush plate 17 to reciprocate, and the brush plate 17 drives the bristles 19 to reciprocate. The bristles 19 are attached to one side of the filter mesh 8, which can remove the abdominal cavity tissue contaminated on the filter mesh 8 to prevent it from clogging the filter mesh 8 and increasing the difficulty of liquid aspiration.

[0037] like Figure 6 As shown, an external rod 23 is fixedly installed on one side of the adjusting knob 9, and a limit rod 24 is fixedly installed on one side of the external rod 23. Two limit blocks 25 are fixedly installed on the surface of the needle and tube connector 1 to facilitate limiting the limit rod 24. The two limit blocks 25 are perpendicular to each other. When the limit rod 24 moves from one side of the limit block 25 to the other side of the limit block 25, the adjusting knob 9 can be rotated 90°.

[0038] like Figure 6 As shown, a plurality of extrusion blocks 26 are fixedly mounted on the inner wall of the needle-tube connector 1 for facilitating external connection. The extrusion blocks 26 facilitate external connection of the syringe.

[0039] like Figure 1 As shown, the surface of the adjusting knob 9 is provided with anti-skid grooves, which facilitate the rotation of the adjusting knob 9.

[0040] Working principle of the present invention:

[0041] During use, the present invention is first connected to a syringe filled with PBS buffer, and then the needle 6 is slowly inserted into the abdominal cavity of the experimental mouse. When the specified depth is reached, the adjusting knob 9 is rotated to move the limit rod 24 to the side of another limit block 25. The adjusting knob 9 drives the wrapping shell 3 to rotate, and the wrapping shell 3 drives the threaded guide tube 4 to rotate. The threaded guide tube 4 drives the needle 6 under the limiting and guiding action of the hexagonal rod 5, so that the needle 6 begins to retract. At the same time, the connecting port 7 rotates together and gradually aligns with the suction port 28. When the needle 6 is completely retracted, the connecting port 7 is aligned with the suction port 28. At this time, the syringe is pushed, and the PBS buffer in the syringe is gradually squeezed into the abdominal cavity of the mouse through the syringe body 2 and the suction port 28, completing the injection of the PBS buffer.

[0042] Pull out the needle tube body 2, and massage the mouse abdomen. After a few minutes, start to add PBS buffer. First, reversely rotate the adjusting knob 9 to extend the needle 6, and then puncture the mouse's abdominal cavity through the needle 6. At this time, rotate the adjusting knob 9 again to hide the needle 6 and align the suction port 28 with the communication port 7. The spring 29 drives the slide 10 to move under the action of the telescopic rod 13. The slide 10 drives the hemispherical clamping block 11 to move. The hemispherical clamping block 11 is inserted into the clamping groove 12 to complete the limit of the adjusting knob 9, thereby completing the limit of the wrapping shell 3 and the needle 6 to prevent the needle 6 from shaking during subsequent operations, causing the needle 6 to penetrate too deeply and injure the intestinal wall, causing contamination and leading to the failure of the experiment. Then start to pull the syringe in the opposite direction to suck out the PBS buffer;

[0043] During aspiration, the filter screen 8 provided in the suction port 28 can effectively prevent the intra-abdominal tissue from directly entering the needle tube body 2, blocking the needle tube body 2 and increasing the difficulty of aspiration. At the same time, during the aspiration of PBS buffer, the PBS buffer flows through the water wheel 22, causing the water wheel 22 to rotate, and the water wheel 22 drives the rotating rod 18 to rotate, and the rotating rod 18 drives the rotating disk 20 to rotate, and the rotating disk 20 drives the driving block 21 to rotate. The driving block 21 drives the reciprocating block 16 to reciprocate through the rectangular opening 27 under the action of the four connecting rods 14. The reciprocating block 16 drives the brush plate 17 to reciprocate, and the brush plate 17 drives the bristles 19 to reciprocate. The bristles 19 are attached to one side of the filter screen 8, which can remove the abdominal tissue contaminated on the filter screen 8 to prevent it from clogging the filter screen 8 and increasing the difficulty of aspiration. Finally, all the PBS buffer is extracted, and the irrigation and sampling are completed.

[0044] Finally, it should be noted that the above are only preferred embodiments of the present invention and are not intended to limit the present invention. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art can still modify the technical solutions described in the aforementioned embodiments or make equivalent replacements for some of the technical features therein. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention should be included in the scope of protection of the present invention.

Claims

1. A needle for peritoneal lavage in mouse peritoneal macrophage isolation, comprising a needle-tube connector (1), characterized in that: A mounting opening is provided on one side of the needle tube connector (1), a needle tube body (2) is fixedly mounted in the mounting opening, a suction port (28) is provided on the surface of the needle tube body (2), and a puncture assembly for puncture is rotatably mounted on the surface of the needle tube body (2); The puncture assembly comprises a wrapping shell (3), the wrapping shell (3) is rotatably mounted on the surface of the needle tube connector (1), a connecting port (7) corresponding to the suction port (28) is provided on the surface of the wrapping shell (3), a hexagonal rod (5) is fixedly mounted on one end of the needle tube body (2), a needle (6) for puncture is slidably mounted on the surface of the hexagonal rod (5), a threaded guide tube (4) is fixedly mounted on one side of the wrapping shell (3), the surface of the needle (6) is threadedly mounted on the inner wall of the threaded guide tube (4), a filter screen (8) is fixedly mounted in the suction port (28) to prevent the needle tube body (2) from being blocked, a positioning component is fixedly mounted on the surface of the wrapping shell (3) to facilitate positioning, and an anti-blocking component is fixedly mounted on the inner wall of the needle tube body (2) to prevent the filter screen (8) from being blocked.

2. A needle for peritoneal lavage of mouse peritoneal macrophage isolation according to claim 1, characterized in that: The positioning assembly comprises an adjusting knob (9), the adjusting knob (9) being fixedly mounted on the surface of the wrapping shell (3), a mounting groove being provided on one side of the adjusting knob (9), a slide plate (10) being slidably mounted in the mounting groove, a hemispherical clamping block (11) being fixedly mounted on one side of the slide plate (10), a plurality of clamping grooves (12) for facilitating clamping being provided on one side of the needle tube connector (1), and a reset unit being fixedly mounted on the other side of the slide plate (10).

3. A needle for peritoneal lavage of mouse peritoneal macrophage isolation according to claim 2, characterized in that: The reset unit comprises a telescopic rod (13), one end of the telescopic rod (13) is fixedly mounted on one side of the slide (10), the other end of the telescopic rod (13) is fixedly mounted on the inner wall of the mounting groove, and a spring (29) is sleeved and mounted on the surface of the telescopic rod (13).

4. A needle for peritoneal lavage of mouse peritoneal macrophage isolation according to claim 3, characterized in that: The anti-blocking assembly comprises a plurality of connecting rods (14), one end of each of the connecting rods (14) being fixedly mounted with a same mounting plate (15), a reciprocating block (16) being slidably mounted on the surface of the mounting plate (15) via the plurality of connecting rods (14), a brush plate (17) being fixedly mounted on one side of the reciprocating block (16), a plurality of bristles (19) for cleaning the filter screen (8) being fixedly mounted on the inner wall of the brush plate (17), and a power unit being fixedly mounted on the inner wall of the needle tube body (2).

5. A needle for peritoneal lavage in mouse peritoneal macrophage isolation according to claim 4, characterized in that: The power unit comprises a rotating rod (18), the rotating rod (18) is rotatably mounted on the inner wall of the needle tube body (2), a water wheel (22) is fixedly mounted on the surface of the rotating rod (18), a rotating disk (20) is fixedly mounted on one end of the rotating rod (18), a circular opening is opened on one side of the mounting plate (15) to facilitate the movement of the rotating disk (20), a driving block (21) is fixedly mounted on the surface of the rotating disk (20), a rectangular opening (27) is opened on one side of the reciprocating block (16), and the driving block (21) is slidably connected to the inner wall of the rectangular opening (27).

6. A needle for peritoneal lavage in mouse peritoneal macrophage isolation according to claim 2, characterized in that: An external connecting rod (23) is fixedly mounted on one side of the adjusting knob (9), a limiting rod (24) is fixedly mounted on one side of the external connecting rod (23), and two limiting blocks (25) for facilitating limiting the limiting rod (24) are fixedly mounted on the surface of the needle tube connector (1).

7. The needle for peritoneal lavage of mouse peritoneal macrophage isolation according to claim 1, characterized in that: A plurality of extrusion blocks (26) are fixedly mounted on the inner wall of the needle tube connector (1) for facilitating external connection.

8. The needle for peritoneal lavage of mouse peritoneal macrophage isolation according to claim 1, characterized in that: The surface of the threaded guide tube (4) is designed to be inclined so as to fit in with the surface of the needle (6).

9. The needle for peritoneal lavage of mouse peritoneal macrophage isolation according to claim 2, characterized in that: The surface of the adjusting knob (9) is provided with an anti-slip groove.

10. The needle for peritoneal lavage of mouse peritoneal macrophage isolation according to claim 4, characterized in that: The connecting rod (14) is in a dumbbell shape.