A method for using microorganisms to reduce saline water into seawater suitable for aquaculture of various seafood, and the application of fish fertilizer specifically for aquaculture.

Abstract

This invention discloses a method for using microorganisms to reduce saline water to modern seawater suitable for aquaculture of various seafood, and the application of a special aquaculture fertilizer. Specific steps include: 1. Saline water pretreatment: Pumping saline water with a salinity of 15‰-30‰ into the aquaculture pond, mixing with air for sedimentation, achieving a pH of 7.3-7.5; 2. Microbial culture: Multi-stage enrichment of *Halobacterium violaceum* colonies to select dominant colonies, mixing groundwater with a salinity of 10-20‰, saline water from salt lakes with a salinity of 180-200‰, and high-salinity soil containing organic matter, for 20... The process involves steaming and sealing at 0-210℃, inoculating with selected purple mixed halobacteria, removing air, culturing under sunlight, and propagating for later use, with a pH of 8.3-8.5; third, reduction reaction: the reduction reaction is carried out in a still water environment. When the water turns reddish-brown or tea-brown, heterotrophic photosynthetic bacteria liquid, autotrophic photosynthetic bacteria liquid, and yeast powder are inoculated and reacted together. Then, aquaculture-specific fish fertilizer is added to increase oxygenation, raising the pH to 8.5-8.8; this invention can utilize the saline-alkali water of deserts and Gobi to cultivate genuine seafood at low cost.

Description

Technical Field

[0001] This invention relates to a method for cultivating various seafood using microorganisms, and more particularly to a method for using microorganisms to reduce saline water to seawater suitable for cultivating various seafood, as well as the application of aquaculture-specific fish fertilizer. Background Technology

[0002] Currently, the components of modern seawater can be classified into five categories: 1. Major components (major and macroelements): These refer to components with a concentration greater than 1 mg / kg in seawater. Cations such as sodium (Na) belong to this category. + K + Ca 2+ Mg 2+ and Sr 2+ Five types, anions Cl - SO4 2- ,Br - HCO3 - CO3 2- F - There are six types of elements, plus H3BO3 in molecular form, which together account for 99.9% of seawater salinity. Therefore, they are called the major components. Because these components are present in large quantities in seawater, and their concentration ratios are approximately constant, biological activity and changes in total salinity have little impact on them; therefore, they are called conserved elements. The Si content in seawater sometimes exceeds 1 mg / kg, but because its concentration is greatly affected by biological activity and its properties are unstable, it is considered a non-conserved element and is not included in discussions of major components. 2. Dissolved gaseous components in seawater, such as oxygen, nitrogen, and inert gases. 3. Nutrient elements (nutrient salts, biogenic elements): mainly elements related to marine plant growth, usually referring to N, P, and Si. The content of these elements in seawater is often affected by plant activity; when their content is very low, it can limit normal plant growth, so these elements are of great importance to organisms. 4. Trace elements: those present in very low quantities in seawater but not classified as nutrients. 5. Organic matter in seawater: such as amino acids, humic substances, chlorophyll, etc.

[0003] Although seawater contains various dissolved salts, the formation of seawater salinity is a complex issue related to the origin of the Earth, the formation of the ocean, and its evolution. It is generally believed that salt primarily originates from weathering products of Earth's crustal rocks and soluble compounds ejected from submarine volcanoes. Based on its origins, seawater should seem to contain all elements found on Earth; however, due to limitations in analytical techniques, only about 80 have been measured. Some low-content components are difficult to measure, and few samples have been tested, making it difficult to represent their average concentration. Many metals of interest are present in extremely low concentrations in seawater and can only be measured using sensitive testing instruments and techniques, while avoiding contamination during sample collection and analysis.

[0004] Looking at my country's aquaculture industry, traditional farming methods generally involve using chemical fertilizers to cultivate aquatic organisms and chemical bottom conditioners or mineral water conditioners to treat the environment. The process typically involves mixing chemical fertilizers with common microbial agents or probiotics and applying the mixture. Specifically, each application uses 20-30 catties of chemical fertilizer per mu (approximately 0.067 hectares) with 1-2 catties of common microbial agents or probiotics, usually 10-12 times per month, along with 5-6 applications of chemical or mineral conditioners. Scientific analysis shows that the utilization rate of chemical fertilizers and chemical minerals in this method is only 10-20%, with the remainder settling at the bottom of ponds and flowing into inland rivers and the sea. my country's inland and coastal waters are already suffering from varying degrees of pollution and eutrophication. Therefore, protecting our natural environment and the oceans that support our thriving industry is everyone's inescapable responsibility.

[0005] In conclusion, in addition to protecting the world's natural aquaculture water sources, it is very important to find other natural water sources suitable for seafood farming.

[0006] The pH value of saline-alkali water is generally above 8. The pH value of saline-alkali water is affected by its salinity and chemical composition, and it usually exhibits alkaline characteristics. The salinity of saline-alkali water is generally between 5‰ and 30‰, a range that limits the growth of plants and animals. The formation of saline-alkali land is often related to the evaporation of groundwater and surface water. During this process, the salt concentration in the water gradually increases, leading to alkalinity. Changes in the pH value of saline-alkali water have a significant impact on the organisms living in it. For example, the suitable pH range for fish is usually between 6.5 and 8.5. When the pH value of the water exceeds this range, especially deviating significantly towards alkalinity, fish may experience discomfort or even death. This is because changes in pH value affect the chemical balance in the water, thereby affecting the toxicity of chemicals in the water. For example, when the pH value rises, ammonia nitrogen in the water exists in the form of non-ionic ammonia. This form of ammonia is highly toxic to fish because it is highly lipid-soluble and can penetrate cell membranes to poison cells. Therefore, understanding and controlling the pH value of saline-alkali water bodies is crucial for protecting the health of aquatic organisms. In practical applications, measures may need to be taken to adjust the pH value of water bodies to ensure it is within a suitable range, thereby maintaining the stability of the ecosystem and the living conditions of organisms.

[0007] Tests on saline lake water and groundwater in the Xinjiang Uygur Autonomous Region revealed a salinity of 270‰ for the lake water and 8‰ for the groundwater. The 270‰ salinity of the lake water is unsuitable for marine life. Even after reconstitution into seawater, the 8‰ salinity of the groundwater only allows for the cultivation of low-end "fake seafood," such as whiteleg shrimp, giant freshwater prawns, and California bass. High-end, genuine seafood like grouper, tiger grouper, pearl grouper, yellowfin bream, lobster, and abalone cannot survive in this environment. In the Xinjiang Uygur Autonomous Region, almost no fish survive in the numerous salt lakes because their composition is primarily composed of sodium chloride and nitrates, which are unsuitable for marine life.

[0008] In the vast world of food, seafood, river fish, and freshwater fish have always been beloved delicacies. However, not everyone knows their exact definitions. Seafood lives in marine environments, river fish inhabit brackish water environments, and freshwater fish grow in freshwater environments.

[0009] True seafood, in the strictest sense, must grow and be farmed in seawater. Fish, shrimp, and crab species that survive in waters with a salinity of 15‰ to 20‰ or higher can generally be called seafood. Examples include various types of grouper such as pearl grouper and red grouper, yellowfin seabream, mudskipper, golden pomfret, golden croaker, large yellow croaker, amberjack, and tuna, as well as abalone, sea cucumber, swimming crab, lobster, mantis shrimp, and tiger prawns. Fish, shrimp, and crabs living in brackish waters where rivers meet the sea with a salinity below 8‰ are called river delicacies.

[0010] Fish, shrimp, and crabs that grow in saline water with a salinity below 3‰ and freshwater with a salinity below 0‰ are all classified as freshwater delicacies. It is important to emphasize that only a very few species can adapt to freshwater environments with a salinity of 3‰ and in freshwater conditions, such as whiteleg shrimp, California bass, mitten crab, mud crab, giant freshwater prawn, eel, and salmon. Because these species grow in freshwater environments, they should, by definition, be classified as freshwater delicacies. Furthermore, there are significant differences in taste and quality between seafood, freshwater delicacies, and river delicacies.

[0011] Clearly and accurately defining seafood, river fish, and freshwater fish not only helps us to know the source and characteristics of the ingredients more precisely.

[0012] In order to develop a method and process to restore the saline water of Xinjiang Uygur Autonomous Region to modern seawater suitable for the cultivation of various seafood, the inventor spent three years traveling throughout the southern region of Xinjiang Uygur Autonomous Region. He found that various aquaculture units were trying to add fresh water to the saline water and then add various seawater elements.

[0013] The disadvantage of this method, which involves adding fresh water to saline-alkali water and then adding various seawater elements, is that:

[0014] 1) This method is very costly.

[0015] The steps for this method of adding seawater elements to freshwater are as follows: (Prepare 10-12‰ seawater)

[0016] 1. 12 cubic meters of fresh water intake;

[0017] 2. Add 62.5 kg of coarse salt;

[0018] 3. 35 kg of magnesium sulfate;

[0019] 4 kg of calcium chloride;

[0020] 5. Potassium chloride 2 kg;

[0021] 6. Sodium octaborate tetrahydrate 1.21 kg;

[0022] 7. Potassium bromide 180g;

[0023] 8. Phosphoric acid 25g;

[0024] 9. On the first day after releasing the seedlings, add 5kg of lactic acid, 2 scoops of algae, and 200g of quick-acting supplement (costing 25 yuan). Add 200g of multivitamins (costing 25 yuan) 10 minutes before releasing the seedlings.

[0025] Cost calculation is as follows: (Prepared with 10-12‰ seawater)

[0026] 1. 12 cubic meters of fresh water intake;

[0027] 2. 62.5 kg of coarse salt × 2 yuan = 125 yuan;

[0028] 3. Magnesium sulfate 35kg × 1.6 yuan = 56 yuan;

[0029] 4. Calcium chloride 4kg × 2 yuan = 8 yuan;

[0030] 5. Potassium chloride 2kg × 3 yuan = 6 yuan;

[0031] 6. Sodium octaborate tetrahydrate 1.21kg × 10 yuan = 12.1 yuan;

[0032] 7. Potassium bromide 180g = 5 yuan;

[0033] 8. 25g of phosphoric acid = 5 yuan;

[0034] 9. On the first day after releasing the seedlings, add 5kg of lactic acid (10 yuan = 50 yuan), 2 scoops of algae, and 200g of quick-acting supplement (25 yuan). Add 200g of multivitamins (25 yuan) 10 minutes before releasing the seedlings. The total cost of the materials is approximately 317.1 yuan.

[0035] 317.19 ÷ 12 cubic meters of water = 26.425 / cubic meter of water.

[0036] For example, a 15-mu pond with a water depth of 1 meter contains approximately 10,000 cubic meters of seawater at a rate of 10-12‰, which costs 26.425 yuan, totaling 264,250 yuan.

[0037] The above formula can prepare seawater with a specific gravity of 1.0-1.2% (10-12 parts per thousand). The exact amount may vary depending on the composition of the freshwater used, but the difference is not significant. For seafood farming, a specific gravity of 20-22‰ seawater is required. For a 15-acre pond with a water depth of 1 meter and approximately 10,000 cubic meters of seawater, the cost would be 528,500 yuan.

[0038] 2) This method cannot be used to farm seafood; it can only be used to farm river fish, not real seafood.

[0039] 3) Because the Xinjiang Uygur Autonomous Region does not allow the indiscriminate discharge of water after aquaculture, and the terrain is generally desert and Gobi, there are no rivers to discharge into. Without changing the water, the aquaculture ponds cannot achieve zero pollution and zero discharge, so the effect of aquaculture is extremely poor, the survival rate is low, and it is even more impossible to aquaculture real seafood.

[0040] 4) Fish fry must be domesticated before they can be raised in this way. Summary of the Invention

[0041] The purpose of this invention is to provide a method for using microorganisms to reduce saline water into modern seawater suitable for aquaculture, and the application of a special aquaculture fertilizer. It is not only a pure natural and green biological agent and an ideal substitute for antibiotics, but also can effectively improve the yield, quality, feed conversion rate and economic benefits of aquatic products.

[0042] To solve the above-mentioned technical problems, the technical solution of the present invention is as follows:

[0043] A method for using microorganisms to reduce saline water to seawater suitable for cultivating various seafood includes the following steps:

[0044] First, pretreatment of saline-alkali water:

[0045] A saline-alkali water with a salinity of 15‰-30‰ (by mass) is pumped into the aquaculture pond, and an air compressor is turned on to fully mix the air with the saline-alkali water, promoting the precipitation of some substances for subsequent treatment. The pH value controlled during the pretreatment process of the saline-alkali water is 7.3-7.5.

[0046] Second, microbial culture:

[0047] Purple halobacterium colonies that survive in high-salt-alkali coastal environments and purple halobacterium colonies that survive in high-altitude salt lakes were selected separately. After multi-stage enrichment, the dominant halobacterium colonies of each were selected and mixed to form a purple halobacterium strain for later use.

[0048] A mixture is prepared by mixing saline water from saline-alkali ground with a salinity of 10-20‰ (mass per thousand), saline water from salt lakes with a salinity of 180-200‰ (mass per thousand), and high-salinity soil containing some organic matter. This mixture is then boiled at 200-210℃, sealed in a light-transmitting incubator, and inoculated with the selected purple mixed halobacillus strain from step 1. The air inside the incubator is removed, and the mixture is cultured under sunlight. When the substrate appears purple, it is ready for propagation and use. The pH value controlled during inoculation is 8.3-8.5.

[0049] Third, reduction reaction:

[0050] The mixed purple halophilic bacteria cultured and propagated in the second step above are inoculated into the saline water of the aquaculture pond described in the first step above, and the waterwheel equipment is turned on and stirred for two hours before being turned off.

[0051] The reduction reaction is carried out in still water. After 5-10 days, when the water appears reddish-brown or tea-brown, heterotrophic photosynthetic bacteria solution, autotrophic photosynthetic bacteria solution, and yeast powder are introduced and reacted together for 5-10 days. Then, a special aquaculture fertilizer preparation is introduced, and the oxygenation equipment is turned on 24 hours a day for 3 days to control the pH value from 7.3-7.5 to 8.5-8.8.

[0052] Fourth, water quality monitoring and adjustment:

[0053] Real-time monitoring of various indicators of the saline-alkali water after reduction; adjustment of the inoculation amount and types of microorganisms within the range of the first to third steps above, based on the biological characteristics and needs of seafood; and detection of the saline-alkali water reduction and transformation process to ensure that the water quality meets the requirements of various seafood farming.

[0054] Fifth, farmed seafood:

[0055] The restored saline water was used in aquaculture experiments. First, sea fish fry, shrimp fry, crab fry, and shellfish fry were tested. When more than 90% of them survived after 48 hours, the restoration of seawater was confirmed to be successful.

[0056] The method described above for using microorganisms to reduce saline water to modern seawater suitable for cultivating various seafood includes the following specific steps:

[0057] First, pretreatment of saline-alkali water:

[0058] Pump 6677-6688 cubic meters of saline-alkali water with a salinity of 15‰-30‰ (by mass) into the aquaculture pond and turn on the air compressor to fully mix the air with the saline-alkali water, promoting the precipitation of some substances for subsequent treatment. The pH value controlled during the pretreatment process of the saline-alkali water is 7.3-7.5.

[0059] Second, microbial culture:

[0060] Purple halobacterium colonies that survive in high-salt-alkali coastal environments and purple halobacterium colonies that survive in high-altitude salt lakes were selected separately. After multi-stage enrichment, the dominant halobacterium colonies of each were selected and mixed to form 500-510 grams of purple halobacterium strain for later use.

[0061] Mix 5000-5010 grams of saline-alkali water from saline-alkali ground with a salinity of 10-20‰ (mass per thousand), 5000-5010 grams of saline-alkali water from a salt lake with a salinity of 180-200‰ (mass per thousand), and 500-510 grams of high-salt-alkali soil containing some organic matter to obtain a mixture. After steaming this mixture at 200-210℃, put it into a light-transmitting incubator and seal it. Inoculate it with the purple mixed halobacterium strain selected in the first step above, remove the air from the incubator, and cultivate it using sunlight. When the substrate appears purple, it can be propagated and used for later use. The pH value controlled during inoculation and cultivation is 8.3-8.5.

[0062] Third, reduction reaction:

[0063] The mixed purple halophilic bacteria cultured and propagated in the second step above are inoculated into the saline water of the breeding pond described in the first step at a rate of 10-20 grams per cubic meter of saline water. The waterwheel equipment is turned on and stirred for two hours before being turned off.

[0064] The reduction reaction is carried out in still water. After 5-10 days, when the water appears reddish-brown or tea-brown, add 10-20 grams of heterotrophic photosynthetic bacteria solution (3-5 billion CFU / mL) and 10-20 grams of autotrophic photosynthetic bacteria solution (3-5 billion CFU / mL) and 10-20 grams of yeast powder (10 billion CFU / g) per cubic meter of water. After reacting together for 5-10 days, add 30-31 grams of a special aquaculture fertilizer preparation per cubic meter of water. The oxygenation equipment is turned on 24 hours a day for 3 days, and the pH is controlled to rise from 7.3-7.5 to 8.5-8.8.

[0065] Fourth, water quality monitoring and adjustment:

[0066] Real-time monitoring of various indicators of the saline-alkali water after reduction; adjustment of the inoculation amount and types of microorganisms within the range of the first to third steps above, based on the biological characteristics and needs of seafood; and detection of the saline-alkali water reduction and transformation process to ensure that the water quality meets the requirements of various seafood farming.

[0067] Fifth, farmed seafood:

[0068] The restored saline water was used in aquaculture experiments. First, sea fish fry, shrimp fry, crab fry, and shellfish fry were tested. When more than 90% of them survived after 48 hours, the restoration of seawater was confirmed to be successful.

[0069] The aforementioned special aquaculture fertilizer preparation is introduced, and the oxygenation equipment is turned on 24 hours a day. After 3 days of reaction, beneficial mixed concentrated algae solution and beneficial zooplankton species are introduced.

[0070] The re-introduction of beneficial mixed concentrated algae solution and beneficial zooplankton species refers to the re-introduction of 50 grams of beneficial mixed concentrated algae solution including: brown finger algae, marine green algae, and coral algae per cubic meter of water, and the re-introduction of 50 grams of live beneficial zooplankton including copepods: water flea and water flea per cubic meter of water.

[0071] The pH value for the fourth step of water quality monitoring and adjustment and the fifth step of seafood farming is 8.5-8.8.

[0072] The various seafood mentioned include: golden pomfret, golden flounder, pearl grouper, East Star grouper, yellowfin bream, large oyster, East China Sea pearl, sea mullet, mudskipper, and seaweed.

[0073] The various seafoods mentioned do not require a domestication process and can be directly introduced into aquaculture.

[0074] The aquaculture-specific fish fertilizer mentioned above is composed of the following raw materials mixed in the indicated weight ratios:

[0075] The inoculum of Clostridium thermocellum was 0.1–0.2 parts.

[0076] The inoculum of *Actinomyces melanocyles*, *A. roseus*, and *A. cellulose* was 0.1–0.2 parts each.

[0077] The inoculum of Aspergillus oryzae, Rhizopus, and Trichoderma viride were 0.1–0.2 parts each.

[0078] 18-20 parts wheat bran mixture: Mix wheat bran and sterile water in a 1:1 weight ratio and set aside.

[0079] The inoculum of yeast (Saccharomyces) and white mold (Geotrichum candidum) was 0.1–0.2 parts each;

[0080] Lactic acid bacteria (LAB) and heterologous lactic acid fermentation inoculum were prepared at 0.5–0.6 parts each.

[0081] The inoculum concentrations for photoautotrophic or chemoautotrophic photosynthetic bacteria and heterotrophic purple nitrogen-fixing photosynthetic bacteria were 0.5–0.6 parts, respectively.

[0082] Mix 16-26 parts of bacterial nutrient solution with 1-3 parts of potassium dihydrogen phosphate solution, 1-1.5 parts of calcium chloride, 1-1.5 parts of urea, and 5-7 parts of sugar. Dissolve the mixture in sterile water at a weight ratio of 1:2 and set aside.

[0083] The preparation of the aquaculture-specific fish fertilizer includes the following steps (the quantities are by weight):

[0084] (1) Material preparation:

[0085] ① The inoculum of Clostridium thermophilum is 0.1 to 0.2 parts;

[0086] ② The inoculum of black-red spiral actinomycetes, rose-colored actinomycetes, and fibrous actinomycetes were 0.1 to 0.2 parts respectively;

[0087] ③ The inoculum of Aspergillus oryzae, Rhizopus, and Trichoderma viride were 0.1 to 0.2 parts respectively;

[0088] ④ 18-20 parts wheat bran mixture: Add wheat bran and sterile water in a 1:1 weight ratio and mix well for later use;

[0089] ⑤ Yeast and Geotrichum candida inoculum powder: 0.1-0.2 parts each;

[0090] ⑥ The inoculum for lactic acid bacteria and heterologous lactic acid fermentation bacteria is 0.5-0.6 parts each;

[0091] ⑦ The inoculum of photoautotrophic or chemoautotrophic photosynthetic bacteria and heterotrophic purple nitrogen-fixing photosynthetic bacteria were 0.5–0.6 parts respectively;

[0092] ⑧ Mix 16-26 parts of microbial nutrient solution with 1-3 parts of potassium dihydrogen phosphate solution, 1-1.5 parts of calcium chloride, 1-1.5 parts of urea, and 5-7 parts of sugar. Dissolve the mixture in sterile water at a weight ratio of 1:2 and set aside.

[0093] (2) Production:

[0094] First, mix ingredients ①, ②, and ③ from the above-mentioned materials thoroughly. Then, add ingredient ④ from the above-mentioned materials and mix well. Ferment on an slant surface for 5 days, stirring once in the morning and once in the evening each day. After 5 days, take a sample and dissolve it in water. When the pH value of the solution reaches 9.0, add the fermented material and sterile water at a weight ratio of 1:3.5 and mix well. If the pH value does not reach 9.0, continue fermentation for 2-3 days. Add ingredients ⑤ and ⑥ from the above-mentioned materials and mix well. Then, aerate and ferment for 10 days. After 10 days, when the pH value is 3-4, filter out the bacterial residue, take the bacterial liquid, add ingredient ⑧ from the above-mentioned materials, and mix well. Let it ferment statically for 2 days. Then, add ingredient ⑦ from the above-mentioned materials and mix well. Let it ferment statically for 3 days. After 3 days, package it in buckets to obtain the finished product.

[0095] An application of a special aquaculture fertilizer in the microbial treatment of saline-alkali water to modern seawater aquaculture of various seafood:

[0096] The aquaculture-specific fish fertilizer mentioned above is composed of the following raw materials mixed in the indicated weight ratios:

[0097] The inoculum of Clostridium thermocellum was 0.1–0.2 parts.

[0098] The inoculum of *Actinomyces melanocyles*, *A. roseus*, and *A. cellulose* was 0.1–0.2 parts each.

[0099] The inoculum of Aspergillus oryzae, Rhizopus, and Trichoderma viride were 0.1–0.2 parts each.

[0100] 18-20 parts wheat bran mixture: Mix wheat bran and sterile water in a 1:1 weight ratio and set aside.

[0101] The inoculum of yeast (Saccharomyces) and white mold (Geotrichum candidum) was 0.1–0.2 parts each;

[0102] Lactic acid bacteria (LAB) and heterologous lactic acid fermentation inoculum were prepared at 0.5–0.6 parts each.

[0103] The inoculum concentrations for photoautotrophic or chemoautotrophic photosynthetic bacteria and heterotrophic purple nitrogen-fixing photosynthetic bacteria were 0.5–0.6 parts, respectively.

[0104] Mix 16-26 parts of bacterial nutrient solution with 1-3 parts of potassium dihydrogen phosphate solution, 1-1.5 parts of calcium chloride, 1-1.5 parts of urea, and 5-7 parts of sugar. Dissolve the mixture in sterile water at a weight ratio of 1:2 and set aside.

[0105] The preparation of the aquaculture-specific fish fertilizer includes the following steps:

[0106] (1) Material preparation:

[0107] ① The inoculum of Clostridium thermophilum is 0.1 to 0.2 parts;

[0108] ② The inoculum of black-red spiral actinomycetes, rose-colored actinomycetes, and fibrous actinomycetes were 0.1 to 0.2 parts respectively;

[0109] ③ The inoculum of Aspergillus oryzae, Rhizopus, and Trichoderma viride were 0.1 to 0.2 parts respectively;

[0110] ④ 18-20 parts wheat bran mixture: Add wheat bran and sterile water in a 1:1 weight ratio and mix well for later use;

[0111] ⑤ Yeast and Geotrichum candida inoculum powder: 0.1-0.2 parts each;

[0112] ⑥ The inoculum for lactic acid bacteria and heterologous lactic acid fermentation bacteria is 0.5-0.6 parts each;

[0113] ⑦ The inoculum of photoautotrophic or chemoautotrophic photosynthetic bacteria and heterotrophic purple nitrogen-fixing photosynthetic bacteria were 0.5–0.6 parts respectively;

[0114] ⑧ Mix 16-26 parts of microbial nutrient solution with 1-3 parts of potassium dihydrogen phosphate solution, 1-1.5 parts of calcium chloride, 1-1.5 parts of urea, and 5-7 parts of sugar. Dissolve the mixture in sterile water at a weight ratio of 1:2 and set aside.

[0115] (2) Production:

[0116] First, mix ingredients ①, ②, and ③ from the above-mentioned materials thoroughly. Then, add ingredient ④ from the above-mentioned materials and mix well. Ferment on an slant surface for 5 days, stirring once in the morning and once in the evening each day. After 5 days, take a sample and dissolve it in water. When the pH value of the solution reaches 9.0, add the fermented material and sterile water at a weight ratio of 1:3.5 and mix well. If the pH value does not reach 9.0, continue fermentation for 2-3 days. Add ingredients ⑤ and ⑥ from the above-mentioned materials and mix well. Then, aerate and ferment for 10 days. After 10 days, when the pH value is 3-4, filter out the bacterial residue, take the bacterial liquid, add ingredient ⑧ from the above-mentioned materials, and mix well. Let it ferment statically for 2 days. Then, add ingredient ⑦ from the above-mentioned materials and mix well. Let it ferment statically for 3 days. After 3 days, package it in buckets to obtain the finished product.

[0117] The beneficial effects of this invention are:

[0118] First, it breaks with conventional technology, enabling the cultivation of real seafood in the saline waters of deserts and Gobi as needed. Real seafood includes: golden pomfret, golden tuna, pearl grouper, East Star grouper, yellowfin bream, large oysters, East China Sea pearl, mullet, mudskipper, and seaweed.

[0119] Secondly, it can achieve zero pollution and zero emissions, meeting the necessary conditions for local regulations prohibiting the indiscriminate discharge of aquaculture wastewater into deserts and Gobi areas, thus avoiding the enormous risk of environmental pollution.

[0120] Secondly, it has been proven that the development, implementation, and promotion of this invention are cost-effective. Compared to the high costs of existing technologies—for example, preparing 10,000 cubic meters of seawater at a rate of 10-12‰ costs 264,250 yuan—the cost of this invention for 10,000 cubic meters of water is only 675 yuan. Therefore, it has significant economic benefits.

[0121] Fourth, the reality of industrial pollution of the ocean, especially the nearshore waters, is unavoidable. Finding pristine seawater to farm genuine seafood is a technical challenge in this field. The modern seawater restored in this invention has broad social significance and benefits.

[0122] Fifth, this invention relies entirely on microorganisms, which can effectively inhibit the reproduction of pathogenic bacteria in aquatic animals, solve the problem of pollution in aquaculture, enhance the disease resistance of animals, and maintain the ecological balance of aquatic bodies. It is not only a pure natural and green biological agent and an ideal substitute for antibiotics, but also effectively improves the yield, quality, feed conversion rate, and economic benefits of aquatic products.

[0123] (1) In aquaculture, there is no need to add chemical fertilizers or chemicals to the pond. Simply add a small amount of river water or seawater to the pond periodically, and then apply the microbial preparation of this invention. This will provide the amount of nutrients required by the microorganisms in the pond, cultivate and reproduce abundant primary live bait and microbial species, form a close-knit circular ecological environment and food chain in the water, prevent "pond overturning" and "surfacing", and greatly reduce mortality.

[0124] (2) It can rapidly degrade organic matter and harmful substances. Through the large-scale reproduction and metabolic products of microorganisms in the product, it can quickly and effectively degrade organic matter in water, decompose ammonia nitrogen, nitrite, sulfides and phosphorus and other harmful substances, and protect the health of fish, shrimp and other organisms with its own life (the life activities of the microorganisms themselves) using a purely microbial method.

[0125] (3) It can purify and improve water quality. Through the reproduction of functional microorganisms in the product and their metabolic products, it can eliminate or greatly reduce sedimentation at the bottom of the water, purify and improve water quality, and maintain the ecological balance of the aquatic environment.

[0126] (4) Effectively inhibits pathogens. Highly efficient and viable microorganisms proliferate in the product (theoretically, the number can reach 2 in 24 hours). 72 The beneficial bacteria form a dominant bacterial community, which greatly inhibits the growth and reproduction of pathogenic microorganisms. The beneficial bacteria control the harmful bacteria, enhancing the immunity of aquatic animals and reducing the mortality rate by more than 90%.

[0127] (5) It can significantly improve economic efficiency. The cost-effectiveness and input-output ratio are above 1:10. Attached Figure Description

[0128] 1. Test data of untreated saline-alkali water in Qiemo County, Xinjiang Uygur Autonomous Region, and other areas:

[0129] Figure 1 The data is from September 24-26, 2023.

[0130] Figure 2 The data is from September 26-27, 2023.

[0131] Figure 3 The data is from September 29th to October 1st, 2023.

[0132] Figure 4 This data is from October 1st to 3rd, 2023.

[0133] Figure 5 The data is from October 3-4, 2023.

[0134] 2. Achievements in aquaculture of various seafood in Halajun Township, Artush City, Kizilsu Kyrgyz Autonomous Prefecture, Xinjiang Uygur Autonomous Region:

[0135] Figure 6 This is a rendering of a farmed yellowfin tuna (Yellow-footed tuna).

[0136] Figure 7 This is a rendering of a golden drum fish farm.

[0137] Figure 8 This is a rendering of a farmed golden pomfret.

[0138] Figure 9 This is an illustration of farmed mullet (black mullet).

[0139] Figure 10 This is a picture showing the effect of pearl grouper being farmed.

[0140] Figure 11 This is an illustration of the effects of cultivating pearls from the East China Sea.

[0141] Figure 12 This is a rendering of farmed oysters.

[0142] Figure 13This is a rendering of a farmed giant pearl grouper.

[0143] Figure 14 This is a rendering of a mudskipper farm.

[0144] Figure 15 This is a rendering of seaweed cultivation.

[0145] The test data mentioned in points 1-2 above, as well as the test data of saline-alkali water treated in Kizilsu Kyrgyz Autonomous Prefecture, Xinjiang Uygur Autonomous Region, were all tested using the "CP Water Quality Analysis Kit" produced by CP Group. This water quality analysis kit includes: shrimp kit: ammonia nitrogen, nitrite, pH, total alkali, calcium and magnesium ions, potassium salt, etc. Detailed Implementation

[0146] The inventor has been the technical head of the applicant, Guangxi Sansheng Biotechnology Co., Ltd. On April 25, 2009, he applied for a patent entitled "A Special Fish Fertilizer for Aquaculture and Its Preparation Method", and was granted an invention patent (patent number ZL200910137979.6) by the State Intellectual Property Office on June 13, 2012.

[0147] Xinjiang Uygur Autonomous Region Shishixian Aquatic Products Co., Ltd., at its seafood farming base in Hongqi Farm, Third Division of Xinjiang Production and Construction Corps, has applied the technology and microbial preparations of Guangxi Sansheng Biotechnology Co., Ltd., namely "a method for using microorganisms to reduce saline water to modern seawater suitable for farming various seafood," to its seafood farming, and regards it as a core technology and important farming supplies. Similarly, Qiemo Shishixian Aquatic Products Co., Ltd., at its seafood farming base in Tatirang Township, Qiemo County, Xinjiang Uygur Autonomous Region, has adopted the same technology and microbial preparations as Guangxi Sansheng Biotechnology Co., Ltd., using them as a core technology and important farming supplies for its seafood farming.

[0148] The seafood farming base established by Xinjiang Kizilsu Zhanyu Aquatic Products Co., Ltd. in Halajun Township, Artush City, Kizilsu Kyrgyz Autonomous Prefecture, has adopted the technology and microbial preparations of Guangxi Sansheng Biotechnology Co., Ltd., which are described as "a method of using microorganisms to reduce saline water into seawater suitable for farming various seafood". They regard it as the core technology and important farming supplies for seafood farming.

[0149] The active ingredient involved in this invention is:

[0150] Thermophilic Clostridium thermocellum;

[0151] Note: This bacterium is suitable for high-temperature fermentation. It produces a polycellulase complex in the fermented material, which can dissolve various fibers and lignin. Its solution contains a small amount of ethanol.

[0152] Black-red spiral actinomycetes, Latin name: Actinomyces melanocyles; Rose-colored actinomycetes, Latin name: A. roseus; Fiber actinomycetes, Latin name: A. cellulose;

[0153] Note: These three types of bacteria have basically the same function and properties. They can decompose organic matter and produce extracellular enzymes to break down proteins, cellulose, lignin, and chitin. Therefore, this property can be used to deal with substances with chitinous cell walls. The chitin-degrading enzymes secreted by this bacterial group can dissolve various pathogenic hyphae.

[0154] Aspergillus oryzae;

[0155] Note: This bacterium can produce saccharifying mold, cellulose mold, and phytic acid mold in fermentation materials. It can degrade linear and branched inoculum powder, crude fiber, and phytic acid into various fine molecular and free substances, and degrade macromolecular proteins into peptone, polypeptides, and various amino acids.

[0156] Rhizopus, a fungus with the Latin name Rhizopus;

[0157] Note: This bacterium is a fermentation aid in molds, and its secretions can help catalyze decomposition during mold fermentation.

[0158] Trichoderma viride:

[0159] Note: This fungus is a glucan glycoside hydrolysant (enzyme). It has a very strong catalytic effect on the hydrolysis of cellulose and lignin. Yeast, its Latin name is *Saccharomyces*, and *Geotrichum candidum*.

[0160] Note: These two types of bacteria have basically the same function and properties. They can use the nutrients in the fermentation substrate to synthesize their own proteins and B vitamins, and convert various organic wastewaters into bacterial proteins, vitamins, various enzymes, hormones, etc.

[0161] Lactic acid bacteria, its Latin name is: LAB. Lactic acid bacteria;

[0162] Note: This bacterium can decompose carbohydrates in materials to form lactic acid, playing a balancing role in multi-strain fermentation.

[0163] heterologous lactic acid fermentation bacteria:

[0164] Note: This bacterium can secrete acetic acid, succinic acid, hydrogen, and carbon dioxide into the material during multi-microbial fermentation, providing nutrients for other microbial groups and playing a coordinating role in purifying the environment.

[0165] Photoautotrophic or chemoautotrophic photosynthetic bacteria:

[0166] Description: This bacterium absorbs inorganic matter as its own nutrition, transforms and utilizes ammonia nitrogen, nitrite, and hydrogen sulfide in the water, effectively improves water quality, balances pH value, and accelerates the cycle of nutrients in the water.

[0167] Heterotrophic purple nitrogen-fixing photosynthetic bacteria:

[0168] Note: This bacterium uses light as its energy source and various organic substances as its primary carbon source. It can assimilate various organic substances to form its own substances, and in water bodies, it can absorb and assimilate a large number of fine molecular organic substances. It is of great significance in preventing other harmful bacteria from transforming organic matter into harmful subspecies forms, and in wastewater treatment and environmental purification.

[0169] The process of implementing the technology of this invention:

[0170] 1. Successful seawater aquaculture was achieved by utilizing underground saline-alkali water and underground brine mines around the desert in Ruoqiang County, Bayingolin Mongol Autonomous Prefecture, Xinjiang Uygur Autonomous Region.

[0171] 2. In Qiemo County, located deep in the Taklamakan Desert, marine fish have ventured into the desert and transformed it into their home. 100,000 high-quality seafood species, including pearl clams, mullet, grouper, and shad, have been successfully raised in the desert, with a survival rate exceeding 99%. This is a major breakthrough in seafood farming in the desert, following the success of seafood farming in the Gobi Desert. At the "Shi Shi Xian" aquatic and seafood farming base in Tatirang Town, Qiemo County, the outdoor temperature reaches 43℃, with waves of heat rolling in. Workers operate the power supply to start the aerators and fully automatic feeders in the fishponds. Deep in the desert, the water in the fishponds churns and surges with the aerators, and the fish eagerly leap and frolic for food. The farming base covers 20 acres and includes two fishponds, a breeding pond, and a standardized seafood production workshop. The fishponds contain 100,000 precious species such as golden pomfret, grouper, mullet, mudskipper, yellowtail, and shad. While raising marine fish, the base has also released 2 million pearl oyster seedlings and some oysters into the fishponds for trial farming. The first batch of marine fish will be available in large quantities by the end of this year and sold throughout the country. The desert aquaculture farm was successful in the first half of 2024, and seedlings are now being released again.

[0172] 3. Beida Lake natural salt is truly a gift from nature. Because the lake salt is formed in a natural environment, its crystals contain rich trace elements, such as calcium, magnesium, iron, and potassium. The composition of these trace elements is an important "fingerprint" of the lake salt and plays a key role in human health.

[0173] The Lop Nur potash deposit (a super-large potash deposit, the Lop Nur dry salt lake brine potash deposit in Xinjiang Uygur Autonomous Region) is China's largest sulfate-type brine potash deposit. It is located in the northern part of Lop Nur, at the eastern end of the Tarim Basin in Xinjiang Uygur Autonomous Region. Specifically, it is located in Ruoqiang County, Bayingolin Mongol Autonomous Prefecture, Xinjiang Uygur Autonomous Region, approximately 450 kilometers in a straight line from Korla City to the west and approximately 300 kilometers in a straight line from Bushan County to the north.

[0174] The two locations mentioned above, one for extracting table salt and the other for extracting potassium salt, were used to rehydrate the wastewater after the two salt extractions. This invention was then used to restore the seawater, which was then used to successfully cultivate various types of seafood.

[0175] 4. Groundwater from other places or the drainage ditches of all farms in Xinjiang Uygur Autonomous Region are also collected and restored to modern marine aquaculture for seafood.

[0176] 5. Tuzisuget Salt Lake is approximately 18 kilometers long and 2-4 kilometers wide, covering an area of ​​about 50 square kilometers. The salt layer is 0.5-0.8 meters thick; the crystal size is between 5mm x 8mm and 2mm x 4mm. The salt particles are grayish-green, containing 94% sodium chloride, 0.39% calcium, 0.01% magnesium, 1.02% carbonate, 2.96% dissolved underwater, and 1.1% moisture. The salt reserves are 13.2 million tons, and the sodium sulfate content is 1.2 million tons. This is the location of the Kizilsu Kyrgyz Autonomous Prefecture Zhanyu Company, where aquaculture is currently thriving.

[0177] The following are the test results of untreated saline water in various regions:

[0178] A:

[0179] I. Salt mine soil tested on July 19, 2024, in Tatirang Township, Qiemo County, Bayingolin Mongol Autonomous Prefecture, Xinjiang Uygur Autonomous Region:

[0180] Salinity: 275‰

[0181] pH value: 9.5

[0182] Nitrite: 0

[0183] Ammonia: 0

[0184] Calcium: 100

[0185] Magnesium: 717

[0186] Potassium salt 275

[0187] Total alkalinity: 675.

[0188] II. On July 19, 2024, tests were conducted on the saline-alkali sandy soil at the aquaculture base of Shishixian Aquatic Products Co., Ltd. in Tatirang Township, Qiemo County, Bayingolin Mongol Autonomous Prefecture, Xinjiang Uygur Autonomous Region:

[0189] Salinity 10‰

[0190] pH value 8.7

[0191] Nitrite 0.07

[0192] Ammonia nitrogen 0

[0193] Calcium: 360

[0194] Magnesium: 250

[0195] Potassium: 161

[0196] Total alkali: 304.

[0197] III. Groundwater samples taken on July 19, 2024, from the saline-alkali desert aquaculture base of Shishixian Aquatic Products Co., Ltd. in Tatirang Township, Qiemo County, Bayingolin Mongol Autonomous Prefecture, Xinjiang Uygur Autonomous Region:

[0198] Salinity: 6‰

[0199] pH value: 6.8

[0200] Nitrite: 0

[0201] Ammonia: 0.2

[0202] Hydrogen sulfide: 1.5 mg / L

[0203] Potassium: 155

[0204] Calcium: 160

[0205] Magnesium: 97

[0206] Total alkali: 104.

[0207] IV. Water quality test records of aquaculture ponds at the aquaculture base of Shishixian Aquatic Products Co., Ltd. in Tatirang Township, Qiemo County, Bayingolin Mongol Autonomous Prefecture, Xinjiang Uygur Autonomous Region:

[0208] Pond No. 1:

[0209] Salinity: 22‰

[0210] pH value: 8.6

[0211] Ammonia: 0

[0212] Nitrite: 0.01

[0213] Calcium: 170

[0214] Magnesium: 450

[0215] Potassium salts: 193

[0216] Total alkalinity: 350

[0217] Bicarbonate alkalinity: 250.

[0218] Pond No. 2:

[0219] Salinity: 18.5‰

[0220] pH value: 8.5

[0221] Ammonia: 0

[0222] Nitrite: 0

[0223] Calcium: 160

[0224] Magnesium: 438

[0225] Potassium salt: 156

[0226] Total alkalinity: 370

[0227] Bicarbonate alkalinity: 270.

[0228] B:

[0229] I. Water samples taken from the salt lake in Halajun Township, Artush City, Kizilsu Kyrgyz Autonomous Prefecture, Xinjiang Uygur Autonomous Region on July 21, 2024:

[0230] Salinity: 115‰

[0231] pH value: 8.9

[0232] Nitrite 0.07

[0233] Ammonia: 0

[0234] Calcium: 100

[0235] Magnesium: 717

[0236] Potassium salt: 75

[0237] Total alkalinity: 675.

[0238] II. On July 21, 2024, samples were taken from the Gobi Desert soil at the aquaculture base of Kizilsu Kyrgyz Autonomous Prefecture Zhanyu Aquatic Products Co., Ltd. in Halajun Township, Artux City, Kizilsu Kyrgyz Autonomous Prefecture, Xinjiang Uygur Autonomous Region:

[0239] Salinity: 10‰

[0240] pH value: 9.6

[0241] Nitrite: 0.01

[0242] Ammonia nitrogen: 0

[0243] Calcium: 560

[0244] Magnesium: 250

[0245] Potassium: 261.

[0246] III. Groundwater samples taken on July 22, 2024, at the aquaculture base in Halajun Township, Artux City, Kizilsu Kyrgyz Autonomous Prefecture, Xinjiang Uygur Autonomous Region:

[0247] Salinity: 10‰

[0248] pH value: 8.7

[0249] Nitrite: 0

[0250] Ammonia: 0

[0251] Hydrogen sulfide: 0

[0252] Potassium: 142

[0253] Calcium: 180

[0254] Magnesium: 255

[0255] Total alkali: 586.

[0256] IV. Water quality test record of aquaculture ponds at No. 1 aquaculture base in Halajun Township, Artux City, Kizilsu Kyrgyz Autonomous Prefecture, Xinjiang Uygur Autonomous Region, dated July 23, 2024:

[0257] Area A, Datang (#3)

[0258] Salinity: 20‰

[0259] pH value: 8.5

[0260] Nitrite concentration: 0.01

[0261] Ammonia content: 0

[0262] Calcium ion concentration: 160

[0263] Magnesium ion concentration: 389

[0264] Potassium salt content: 148

[0265] Total alkalinity: 280

[0266] Bicarbonate alkalinity: 180.

[0267] Area B, Datang (#4)

[0268] Salinity: 21‰

[0269] pH value: 8.6

[0270] Nitrite concentration: 0

[0271] Ammonia content: 0

[0272] Calcium ion concentration: 180

[0273] Magnesium ion concentration: 401

[0274] Potassium salt content: 155

[0275] Total alkalinity: 270

[0276] Bicarbonate alkalinity: 170.

[0277] Area C, Datang (#5)

[0278] Salinity: 20.5‰

[0279] pH value: 8.5

[0280] Nitrite concentration: 0.05

[0281] Ammonia content: 0

[0282] Calcium ion concentration: 160

[0283] Magnesium ion concentration: 437

[0284] Potassium salt content: 155

[0285] Total alkalinity: 270

[0286] Bicarbonate alkalinity: 170.

[0287] C:

[0288] I. Well water quality record of Xinjiang Uygur Autonomous Region Shishixian Aquatic Products Co., Ltd.'s base at Hongqi Farm, Third Division of Xinjiang Production and Construction Corps, dated July 6, 2022:

[0289] Salinity: 8‰

[0290] Nitrite 0

[0291] pH value 8.5

[0292] Ammonia 0.2

[0293] Potassium: 55

[0294] Calcium 100

[0295] Magnesium: 480

[0296] Total alkalinity: 504.

[0297] II. Water quality test record of the saline-alkali water in the drainage ditch next to the breeding base of Xinjiang Uygur Autonomous Region Shishixian Aquatic Products Co., Ltd. in Hongqi Farm on July 6, 2022:

[0298] Salinity: 23‰

[0299] Nitrite: 0.25

[0300] pH 8.9

[0301] Ammonia 0.2

[0302] Potassium 242

[0303] Calcium 280

[0304] Magnesium: 560

[0305] Total alkalinity: 650.

[0306] III. Water quality test records of the breeding ponds and factory-style breeding sheds at the breeding base of Xinjiang Uygur Autonomous Region Shishi Fresh Aquatic Products Co., Ltd. in Hongqi Farm, Third Division of Xinjiang Production and Construction Corps, dated May 3, 2023:

[0307] Pond No. 1:

[0308] Salinity: 20‰

[0309] pH value: 8.6

[0310] Ammonia: 0

[0311] Nitrite: 0.01

[0312] Calcium: 170

[0313] Magnesium: 450

[0314] Potassium salts: 173

[0315] Total alkalinity: 350

[0316] Bicarbonate alkalinity: 250.

[0317] Pond No. 2:

[0318] Salinity: 20.5‰

[0319] pH value: 8.5

[0320] Ammonia: 0

[0321] Nitrite: 0.05

[0322] Calcium: 160

[0323] Magnesium: 338

[0324] Potassium salt: 156

[0325] Total alkalinity: 370

[0326] Bicarbonate alkalinity: 270.

[0327] Pond No. 2:

[0328] Salinity: 20.5‰

[0329] pH value: 8.5

[0330] Ammonia: 0

[0331] Nitrite: 0.05

[0332] Calcium: 160

[0333] Magnesium: 338

[0334] Potassium salt: 156

[0335] Total alkalinity: 370

[0336] Bicarbonate alkalinity: 270.

[0337] No. 1 breeding shed:

[0338] Salinity: 19‰

[0339] pH value: 8.4

[0340] Ammonia: 0

[0341] Nitrite: 0.05

[0342] Calcium: 160

[0343] Magnesium: 430

[0344] Potassium salts: 183

[0345] Total alkalinity: 370

[0346] Bicarbonate alkalinity: 260.

[0347] No. 2 breeding shed:

[0348] Salinity: 19.5‰

[0349] pH value: 8.7

[0350] Ammonia: 0

[0351] Nitrite: 0.03

[0352] Calcium: 150

[0353] Magnesium: 385

[0354] Potassium salt: 158

[0355] Total alkalinity: 360

[0356] Bicarbonate alkalinity: 260.

[0357] D:

[0358] Detailed data on untreated saline-alkali water testing in Qiemo County, Xinjiang Uygur Autonomous Region, and other areas are attached. Figure 1-5The untreated saline-alkali water from the aforementioned AD locations was used as the reduction water source for Examples 1-2 below:

[0359] Example 1:

[0360] A method for using microorganisms to reduce saline water to seawater suitable for cultivating various seafood includes the following steps:

[0361] First, pretreatment of saline-alkali water:

[0362] A saline-alkali water with a salinity of 15‰ (by mass) is pumped into the aquaculture pond, and an air compressor is turned on to fully mix the air with the saline-alkali water, promoting the precipitation of some substances for subsequent treatment. The pH value controlled during the pretreatment process of the saline-alkali water is 7.3.

[0363] Second, microbial culture:

[0364] Purple halobacterium colonies that survive in high-salt-alkali coastal environments and purple halobacterium colonies that survive in high-altitude salt lakes were selected separately. After multi-stage enrichment, the dominant halobacterium colonies of each were selected and mixed to form a purple halobacterium strain for later use.

[0365] A mixture is prepared by mixing saline water from saline-alkali ground with a salinity of 10‰ (mass per thousand), saline water from a salt lake with a salinity of 180‰ (mass per thousand), and highly saline-alkali soil containing some organic matter. This mixture is then boiled at 200℃, sealed in a light-transmitting incubator, and inoculated with the selected purple mixed halobacillus strain from step 1. The air inside the incubator is removed, and the mixture is cultured under sunlight. When the substrate appears purple, it is ready for propagation and use. The pH value controlled during inoculation is 8.3.

[0366] Third, reduction reaction:

[0367] The mixed purple halophilic bacteria cultured and propagated in the second step above are inoculated into the saline water of the aquaculture pond described in the first step above, and the waterwheel equipment is turned on and stirred for two hours before being turned off.

[0368] The reduction reaction is carried out in a still water environment. After 5 days, when the water appears reddish-brown or tea-brown, heterotrophic photosynthetic bacteria liquid, autotrophic photosynthetic bacteria liquid, and yeast powder are introduced. After reacting together for 5 days, a special aquaculture fertilizer preparation is introduced. The oxygenation equipment is turned on 24 hours a day for 3 days, and the pH value is controlled to rise from 7.3 to 8.5.

[0369] Fourth, water quality monitoring and adjustment:

[0370] Real-time monitoring of various indicators of the saline-alkali water after reduction; adjustment of the inoculation amount and types of microorganisms within the range of the first to third steps above, based on the biological characteristics and needs of seafood; and detection of the saline-alkali water reduction and transformation process to ensure that the water quality meets the requirements of various seafood farming.

[0371] Fifth, farmed seafood:

[0372] The restored saline water was used in aquaculture experiments. First, sea fish fry, shrimp fry, crab fry, and shellfish fry were tested. When more than 90% of them survived after 48 hours, the restoration of seawater was confirmed to be successful.

[0373] A method for using microorganisms to reduce saline water to modern seawater suitable for cultivating various seafood includes the following specific steps:

[0374] First, pretreatment of saline-alkali water:

[0375] 6677 cubic meters of saline-alkali water with a salinity of 15‰ (parts by mass) were pumped into the aquaculture pond, and the air compressor was turned on to fully mix the air with the saline-alkali water, promoting the precipitation of some substances for subsequent treatment. The pH value controlled during the pretreatment process of the saline-alkali water was 7.3.

[0376] Second, microbial culture:

[0377] Purple halophilus colonies surviving in high-salinity coastal environments and those surviving in high-altitude salt lakes were selected separately. After multi-stage enrichment, the dominant halophilus colonies from each species were selected and mixed to form 500 grams of purple halophilus inoculum for later use.

[0378] 5000g of saline-alkali water from a saline-alkali underground soil with a salinity of 10‰ (mass per thousand), 5000g of saline-alkali water from a salt lake with a salinity of 180‰ (mass per thousand), and 500g of high-salt-alkali soil containing some organic matter were mixed to obtain a mixture. This mixture was then steamed at 200℃, placed in a light-transmitting incubator and sealed. The selected purple mixed halobacillus strain from the first step was inoculated, the air inside the incubator was removed, and the mixture was cultured using sunlight. When the substrate visually turned purple, it was ready for propagation and use. The pH value controlled during inoculation culture was 8.3.

[0379] Third, reduction reaction:

[0380] The mixed purple halophilic bacteria cultured and propagated in the second step above are inoculated into the saline-alkali water of the breeding pond described in the first step at a rate of 10 grams per cubic meter of saline-alkali water. The waterwheel equipment is turned on and stirred for two hours before being turned off.

[0381] The reduction reaction was carried out in still water. After 5 days, when the water appeared reddish-brown or tea-brown, 10 grams of heterotrophic photosynthetic bacteria solution with a concentration of 3 billion CFU / mL, 10 grams of autotrophic photosynthetic bacteria solution with a concentration of 3 billion CFU / mL, and 10 grams of yeast powder with a concentration of 10 billion CFU / mL were added per cubic meter of water. After reacting together for 5 days, 30 grams of special aquaculture fertilizer preparation were added per cubic meter of water. The oxygenation equipment was turned on 24 hours a day for 3 days, and the pH was controlled to rise from 7.3 to 8.5.

[0382] Fourth, water quality monitoring and adjustment:

[0383] Real-time monitoring of various indicators of the saline-alkali water after reduction; adjustment of the inoculation amount and types of microorganisms within the range of the first to third steps above, based on the biological characteristics and needs of seafood; and detection of the saline-alkali water reduction and transformation process to ensure that the water quality meets the requirements of various seafood farming.

[0384] Fifth, farmed seafood:

[0385] The restored saline water was used in aquaculture experiments. First, sea fish fry, shrimp fry, crab fry, and shellfish fry were tested. When more than 90% of them survived after 48 hours, the restoration of seawater was confirmed to be successful.

[0386] The aforementioned special aquaculture fertilizer preparation is introduced, and the oxygenation equipment is turned on 24 hours a day. After 3 days of reaction, beneficial mixed concentrated algae solution and beneficial zooplankton species are introduced.

[0387] The re-introduction of beneficial mixed concentrated algae solution and beneficial zooplankton species refers to the re-introduction of 50 grams of beneficial mixed concentrated algae solution including: brown finger algae, marine green algae, and coral algae per cubic meter of water, and the re-introduction of 50 grams of live beneficial zooplankton including copepods: water flea and water flea per cubic meter of water.

[0388] The pH value for the fourth step of water quality monitoring and adjustment and the fifth step of seafood farming is 8.5.

[0389] The various seafood mentioned include: golden pomfret, golden flounder, pearl grouper, East Star grouper, yellowfin bream, large oyster, East China Sea pearl, sea mullet, mudskipper, and seaweed.

[0390] The various seafoods mentioned do not require a domestication process and can be directly introduced into aquaculture.

[0391] The method described above utilizes microorganisms to reduce saline water to modern seawater suitable for cultivating various seafood. The aquaculture-specific fish fertilizer described above is composed of the following raw materials mixed in the indicated weight ratios:

[0392] The inoculum of Clostridium thermocellum is 0.1 kg.

[0393] The inoculum of *Actinomyces melanocyles*, *A. roseus*, and *A. cellulose* was 0.1 kg each.

[0394] The seed powder of Aspergillus oryzae, Rhizopus, and Trichoderma viride were 0.1 kg each.

[0395] 18 kg of wheat bran mixture: Mix wheat bran and sterile water in a 1:1 weight ratio and set aside.

[0396] The inoculum of yeast (Saccharomyces) and Geotrichum candidum was 0.1 kg each.

[0397] Lactic acid bacteria (LAB. Lactic acid bacteria) and heterologous lactic acid fermentation inoculum: 0.5 kg each.

[0398] The inoculum for photoautotrophic or chemoautotrophic photosynthetic bacteria and heterotrophic purple nitrogen-fixing photosynthetic bacteria was 0.5 kg.

[0399] Mix 16 kg of microbial nutrient solution with 1 kg of potassium dihydrogen phosphate solution, 1 kg of calcium chloride, 1 kg of urea, and 5 kg of sugar. Dissolve the mixture in sterile water at a weight ratio of 1:2 and set aside.

[0400] The method for reducing saline water to modern seawater suitable for aquaculture using microorganisms is characterized by the following steps (parts are by weight):

[0401] (1) Material preparation:

[0402] ① The inoculum of Clostridium thermophilum is 0.1 kg.

[0403] ② The inoculum for black-red spiral actinomycetes, rose-colored actinomycetes, and fibrous actinomycetes was 0.1 kg each.

[0404] ③ The seed powders for Aspergillus oryzae, Rhizopus, and Trichoderma viride were 0.1 kg each.

[0405] ④ 18kg wheat bran mixture: Add wheat bran and sterile water in a 1:1 weight ratio and mix well for later use;

[0406] ⑤ Yeast and Geotrichum cane culture powder: 0.1 kg each.

[0407] ⑥ The inoculum for lactic acid bacteria and heterologous lactic acid fermentation bacteria is 0.5 kg each.

[0408] ⑦ The inoculum for photoautotrophic or chemoautotrophic photosynthetic bacteria and heterotrophic purple nitrogen-fixing photosynthetic bacteria was 0.5 kg.

[0409] ⑧ 16kg of bacterial nutrient solution: Mix 1kg of potassium dihydrogen phosphate solution, 1kg of calcium chloride, 1kg of urea, and 5kg of sugar. Dissolve the mixture in sterile water at a weight ratio of 1:2 and set aside.

[0410] (2) Production:

[0411] First, mix ingredients ①, ②, and ③ from the above-mentioned materials thoroughly. Then, add ingredient ④ from the above-mentioned materials and mix well. Ferment on an slant surface for 5 days, stirring once in the morning and once in the evening each day. After 5 days, take a sample and dissolve it in water. When the pH value of the solution reaches 9.0, add the fermented material and sterile water at a weight ratio of 1:3.5 and mix well. If the pH value does not reach 9.0, continue fermentation for 2 days. Add ingredients ⑤ and ⑥ from the above-mentioned materials and mix well. Then, aerate and ferment for 10 days. After 10 days, when the pH value is 3, filter out the bacterial residue, take the bacterial liquid, add ingredient ⑧ from the above-mentioned materials and mix well. Static fermentation for 2 days. Then, add ingredient ⑦ from the above-mentioned materials and mix well. Static fermentation for 3 days. After 3 days, package the product in buckets to obtain the finished product.

[0412] A special aquaculture fertilizer is used in the microbial treatment of saline-alkali water to reduce it to a suitable form for modern seawater aquaculture of various seafood.

[0413] The aquaculture-specific fish fertilizer mentioned above is composed of the following raw materials mixed in the indicated weight ratios:

[0414] The inoculum of Clostridium thermocellum is 0.1 kg.

[0415] The inoculum of *Actinomyces melanocyles*, *A. roseus*, and *A. cellulose* was 0.1 kg each.

[0416] The seed powder of Aspergillus oryzae, Rhizopus, and Trichoderma viride were 0.1 kg each.

[0417] 18 kg of wheat bran mixture: Mix wheat bran and sterile water in a 1:1 weight ratio and set aside.

[0418] The inoculum of yeast (Saccharomyces) and Geotrichum candidum was 0.1 kg each.

[0419] Lactic acid bacteria (LAB. Lactic acid bacteria) and heterologous lactic acid fermentation inoculum: 0.5 kg each.

[0420] The inoculum for photoautotrophic or chemoautotrophic photosynthetic bacteria and heterotrophic purple nitrogen-fixing photosynthetic bacteria was 0.5 kg.

[0421] Mix 16kg of microbial nutrient solution with 1kg of potassium dihydrogen phosphate solution, 1kg of calcium chloride, 1kg of urea, and 5kg of sugar. Dissolve the mixture in sterile water at a weight ratio of 1:2 and set aside.

[0422] The preparation of the aquaculture-specific fish fertilizer includes the following steps:

[0423] (1) Material preparation:

[0424] ① The inoculum of Clostridium thermophilum is 0.1 kg.

[0425] ② The inoculum for black-red spiral actinomycetes, rose-colored actinomycetes, and fibrous actinomycetes was 0.1 kg each.

[0426] ③ The seed powders for Aspergillus oryzae, Rhizopus, and Trichoderma viride were 0.1 kg each.

[0427] ④ 18kg wheat bran mixture: Add wheat bran and sterile water in a 1:1 weight ratio and mix well for later use;

[0428] ⑤ Yeast and Geotrichum cane culture powder: 0.1 kg each.

[0429] ⑥ The inoculum for lactic acid bacteria and heterologous lactic acid fermentation bacteria is 0.kg.

[0430] ⑦ The inoculum for photoautotrophic or chemoautotrophic photosynthetic bacteria and heterotrophic purple nitrogen-fixing photosynthetic bacteria was 0.5 kg.

[0431] ⑧ 16kg of bacterial nutrient solution: Mix 1kg of potassium dihydrogen phosphate solution, 1kg of calcium chloride, 1kg of urea, and 5kg of sugar. Dissolve the mixture in sterile water at a weight ratio of 1:2 and set aside.

[0432] (2) Production:

[0433] First, mix ingredients ①, ②, and ③ from the above-mentioned materials thoroughly. Then, add ingredient ④ from the above-mentioned materials and mix well. Ferment on an slant surface for 5 days, stirring once in the morning and once in the evening each day. After 5 days, take a sample and dissolve it in water. When the pH value of the solution reaches 9.0, add the fermented material and sterile water at a weight ratio of 1:3.5 and mix well. If the pH value does not reach 9.0, continue fermentation for 2 days. Add ingredients ⑤ and ⑥ from the above-mentioned materials and mix well. Then, aerate and ferment for 10 days. After 10 days, when the pH value is 3, filter out the bacterial residue, take the bacterial liquid, add ingredient ⑧ from the above-mentioned materials and mix well. Static fermentation for 2 days. Then, add ingredient ⑦ from the above-mentioned materials and mix well. Static fermentation for 3 days. After 3 days, package the product in buckets to obtain the finished product.

[0434] Example 2:

[0435] A method for using microorganisms to reduce saline water to seawater suitable for cultivating various seafood includes the following steps:

[0436] First, pretreatment of saline-alkali water:

[0437] A saline-alkali water with a salinity of 30‰ (by mass) is pumped into the aquaculture pond, and the air compressor is turned on to fully mix the air with the saline-alkali water, promoting the precipitation of some substances for subsequent treatment. The pH value controlled during the pretreatment process of the saline-alkali water is 7.5.

[0438] Second, microbial culture:

[0439] Purple halobacterium colonies that survive in coastal high-salt and alkaline environments and purple halobacterium colonies that survive in high-altitude salt lakes were selected respectively. After multi-level enrichment, the dominant halobacterium colonies of each were selected and mixed to form a purple halobacterium strain for later use.

[0440] A mixture is prepared by mixing saline water from a saline-alkali underground soil with a salinity of 20‰ (mass per thousand), saline water from a salt lake with a salinity of 200‰ (mass per thousand), and high-salinity soil containing some organic matter. This mixture is then boiled at 210℃, sealed in a light-transmitting incubator, and inoculated with the selected purple mixed halobacillus strain from step 1. The air inside the incubator is removed, and the mixture is cultured under sunlight. When the substrate appears purple, it is ready for propagation and use. The pH value controlled during inoculation is 8.5.

[0441] Third, reduction reaction:

[0442] The mixed purple halophilic bacteria cultured and propagated in the second step above are inoculated into the saline water of the aquaculture pond described in the first step above, and the waterwheel equipment is turned on and stirred for two hours before being turned off.

[0443] The reduction reaction is carried out in a still water environment. After 10 days, when the water appears reddish-brown or tea-brown, heterotrophic photosynthetic bacteria liquid, autotrophic photosynthetic bacteria liquid, and yeast powder are introduced and reacted together for 10 days. Then, a special aquaculture fertilizer preparation is introduced, and the oxygenation equipment is turned on 24 hours a day for 3 days to control the pH value to rise from 7.5 to 8.8.

[0444] Fourth, water quality monitoring and adjustment:

[0445] Real-time monitoring of various indicators of the saline-alkali water after reduction; adjustment of the inoculation amount and types of microorganisms within the range of the first to third steps above, based on the biological characteristics and needs of seafood; and detection of the saline-alkali water reduction and transformation process to ensure that the water quality meets the requirements of various seafood farming.

[0446] Fifth, farmed seafood:

[0447] The restored saline water was used in aquaculture experiments. First, sea fish fry, shrimp fry, crab fry, and shellfish fry were tested. When more than 90% of them survived after 48 hours, the restoration of seawater was confirmed to be successful.

[0448] A method for using microorganisms to reduce saline water to modern seawater suitable for cultivating various seafood includes the following specific steps:

[0449] First, pretreatment of saline-alkali water:

[0450] 6688 cubic meters of saline-alkali water with a salinity of 30‰ (parts by mass) were pumped into the aquaculture pond, and the air compressor was turned on to fully mix the air with the saline-alkali water, promoting the precipitation of some substances for subsequent treatment. The pH value controlled during the pretreatment process of the saline-alkali water was 7.5.

[0451] Second, microbial culture:

[0452] Purple halobacterium colonies that survive in high-salt-alkali coastal environments and purple halobacterium colonies that survive in high-altitude salt lakes were selected separately. After multi-stage enrichment, the dominant halobacterium colonies of each were selected and mixed to form 510 grams of purple halobacterium strain for later use.

[0453] 5010 grams of saline-alkali water from a saline-alkali underground soil with a salinity of 20‰ (mass per thousand), 5010 grams of saline-alkali water from a salt lake with a salinity of 200‰ (mass per thousand), and 510 grams of high-salt-alkali soil containing some organic matter were mixed to obtain a mixture. This mixture was then steamed at 210℃, placed in a light-transmitting incubator and sealed. The selected purple mixed halobacillus strain from the first step was inoculated, the air inside the incubator was removed, and the mixture was cultured using sunlight. When the substrate visually turned purple, it was ready for propagation and use. The pH value controlled during inoculation culture was 8.5.

[0454] Third, reduction reaction:

[0455] The mixed purple halophilic bacteria cultured and propagated in the second step above are inoculated into the saline-alkali water of the breeding pond described in the first step at a rate of 20 grams per cubic meter of saline-alkali water. The waterwheel equipment is turned on and stirred for two hours before being turned off.

[0456] The reduction reaction was carried out in still water. After 10 days, when the water appeared reddish-brown or tea-brown, 20 grams of heterotrophic photosynthetic bacteria solution with a concentration of 5 billion CFU / mL, 20 grams of autotrophic photosynthetic bacteria solution with a concentration of -5 billion CFU / mL, and 20 grams of yeast powder with a concentration of 10 billion CFU / g were added per cubic meter of water. After reacting together for 10 days, 31 grams of special aquaculture fertilizer preparation were added per cubic meter of water. The oxygenation equipment was turned on 24 hours a day, and the reaction was carried out for 3 days, controlling the pH to rise from 7.5 to 8.8.

[0457] Fourth, water quality monitoring and adjustment:

[0458] Real-time monitoring of various indicators of the saline-alkali water after reduction; adjustment of the inoculation amount and types of microorganisms within the range of the first to third steps above, based on the biological characteristics and needs of seafood; and detection of the saline-alkali water reduction and transformation process to ensure that the water quality meets the requirements of various seafood farming.

[0459] Fifth, farmed seafood:

[0460] The restored saline water was used in aquaculture experiments. First, sea fish fry, shrimp fry, crab fry, and shellfish fry were tested. When more than 90% of them survived after 48 hours, the restoration of seawater was confirmed to be successful.

[0461] The aforementioned special aquaculture fertilizer preparation is introduced, and the oxygenation equipment is turned on 24 hours a day. After 3 days of reaction, beneficial mixed concentrated algae solution and beneficial zooplankton species are introduced.

[0462] The re-introduction of beneficial mixed concentrated algae solution and beneficial zooplankton species refers to the re-introduction of 50 grams of beneficial mixed concentrated algae solution including: brown finger algae, marine green algae, and coral algae per cubic meter of water, and the re-introduction of 50 grams of live beneficial zooplankton including copepods: water flea and water flea per cubic meter of water.

[0463] The pH value for the fourth water quality monitoring and adjustment step and the fifth step of aquaculture is 8.8.

[0464] The various seafood mentioned include: golden pomfret, golden flounder, pearl grouper, East Star grouper, yellowfin bream, large oyster, East China Sea pearl, sea mullet, mudskipper, and seaweed.

[0465] The various seafoods mentioned do not require a domestication process and can be directly introduced into aquaculture.

[0466] The aquaculture-specific fish fertilizer mentioned above is composed of the following raw materials mixed in the indicated weight ratios:

[0467] The inoculum of Clostridium thermocellum is 0.2 kg.

[0468] The inoculum of *Actinomyces melanocyles*, *A. roseus*, and *A. cellulose* was 0.2 kg each.

[0469] The seed powder of Aspergillus oryzae, Rhizopus, and Trichoderma viride were 0.2 kg each.

[0470] 20kg of wheat bran mixture: Mix wheat bran and sterile water in a 1:1 weight ratio and set aside.

[0471] The inoculum of yeast (Saccharomyces) and Geotrichum candidum was 0.2 kg each.

[0472] Lactic acid bacteria (LAB. Lactic acid bacteria) and heterologous lactic acid fermentation inoculum were each 0.6 kg.

[0473] The inoculum for photoautotrophic or chemoautotrophic photosynthetic bacteria and heterotrophic purple nitrogen-fixing photosynthetic bacteria was 0.6 kg.

[0474] Mix 26 kg of bacterial nutrient solution with 3 kg of potassium dihydrogen phosphate solution, 1.5 kg of calcium chloride, 1.5 kg of urea, and 7 kg of sugar. Dissolve the mixture in sterile water at a weight ratio of 1:2 and set aside.

[0475] The preparation of the aquaculture-specific fish fertilizer includes the following steps:

[0476] (1) Material preparation:

[0477] ① The inoculum of Clostridium thermophilum is 0.2 kg.

[0478] ② The inoculum for black-red spiral actinomycetes, rose-colored actinomycetes, and fibrous actinomycetes was 0.2 kg each.

[0479] ③ The seed powder for Aspergillus oryzae, Rhizopus, and Trichoderma viride was 0.2 kg each.

[0480] ④ 20kg wheat bran mixture: Add wheat bran and sterile water in a 1:1 weight ratio and mix well for later use.

[0481] ⑤ Yeast and Geotrichum cane culture powder: 0.2 kg each.

[0482] ⑥ The inoculum for lactic acid bacteria and heterologous lactic acid fermentation bacteria is 0.6 kg each.

[0483] ⑦ The inoculum for photoautotrophic or chemoautotrophic photosynthetic bacteria and heterotrophic purple nitrogen-fixing photosynthetic bacteria was 0.6 kg.

[0484] ⑧ Mix 26kg of microbial nutrient solution with 3kg of potassium dihydrogen phosphate solution, 1.5kg of calcium chloride, 1.5kg of urea, and 7kg of sugar. Dissolve the mixture in sterile water at a weight ratio of 1:2 and set aside.

[0485] (2) Production:

[0486] First, mix ingredients ①, ②, and ③ from the above-mentioned materials thoroughly. Then, add ingredient ④ from the above-mentioned materials and mix well. Ferment on an slant surface for 5 days, stirring once in the morning and once in the evening each day. After 5 days, take a sample and dissolve it in water. When the pH value of the solution reaches 9.0, add the fermented material and sterile water at a weight ratio of 1:3.5 and mix well. If the pH value does not reach 9.0, continue fermentation for 3 days. Add ingredients ⑤ and ⑥ from the above-mentioned materials and mix well. Then, aerate and ferment for 10 days. After 10 days, when the pH value is 4, filter out the bacterial residue, take the bacterial liquid, add ingredient ⑧ from the above-mentioned materials and mix well. Static fermentation for 2 days. Then, add ingredient ⑦ from the above-mentioned materials and mix well. Static fermentation for 3 days. After 3 days, package the product in buckets to obtain the finished product.

[0487] A special aquaculture fertilizer is used in the microbial treatment of saline-alkali water to reduce it to a suitable form for modern seawater aquaculture of various seafood.

[0488] The aquaculture-specific fish fertilizer mentioned above is composed of the following raw materials mixed in the indicated weight ratios:

[0489] The inoculum of Clostridium thermocellum is 0.2 kg.

[0490] The inoculum of *Actinomyces melanocyles*, *A. roseus*, and *A. cellulose* was 0.2 kg each.

[0491] The seed powder of Aspergillus oryzae, Rhizopus, and Trichoderma viride were 0.2 kg each.

[0492] 20kg of wheat bran mixture: Mix wheat bran and sterile water in a 1:1 weight ratio and set aside.

[0493] The inoculum of yeast (Saccharomyces) and Geotrichum candidum was 0.2 kg each.

[0494] Lactic acid bacteria (LAB. Lactic acid bacteria) and heterologous lactic acid fermentation inoculum were each 0.6 kg.

[0495] The inoculum for photoautotrophic or chemoautotrophic photosynthetic bacteria and heterotrophic purple nitrogen-fixing photosynthetic bacteria was 0.6 kg.

[0496] Mix 26 kg of bacterial nutrient solution with 3 kg of potassium dihydrogen phosphate solution, 1.5 kg of calcium chloride, 1.5 kg of urea, and 7 kg of sugar. Dissolve the mixture in sterile water at a weight ratio of 1:2 and set aside.

[0497] The preparation of the aquaculture-specific fish fertilizer includes the following steps:

[0498] (1) Material preparation:

[0499] ① The inoculum of Clostridium thermophilum is 0.2 kg.

[0500] ② The inoculum for black-red spiral actinomycetes, rose-colored actinomycetes, and fibrous actinomycetes was 0.2 kg each.

[0501] ③ The seed powder for Aspergillus oryzae, Rhizopus, and Trichoderma viride was 0.2 kg each.

[0502] ④ 20kg wheat bran mixture: Add wheat bran and sterile water in a 1:1 weight ratio and mix well for later use.

[0503] ⑤ Yeast and Geotrichum cane culture powder: 0.2 kg each.

[0504] ⑥ The inoculum for lactic acid bacteria and heterologous lactic acid fermentation bacteria is 0.6 kg each.

[0505] ⑦ The inoculum for photoautotrophic or chemoautotrophic photosynthetic bacteria and heterotrophic purple nitrogen-fixing photosynthetic bacteria was 0.6 kg.

[0506] ⑧ 26 kg of bacterial nutrient solution: 3 kg of potassium dihydrogen phosphate solution, 1.5 kg of calcium chloride, 1.5 kg of urea, and 7 kg of sugars. Mix the ingredients, and then dissolve the mixture in sterile water at a weight ratio of 1:2.

[0507] (2) Production:

[0508] First, mix ingredients ①, ②, and ③ from the above-mentioned materials thoroughly. Then, add ingredient ④ from the above-mentioned materials and mix well. Ferment on an slant surface for 5 days, stirring once in the morning and once in the evening each day. After 5 days, take a sample and dissolve it in water. When the pH value of the solution reaches 9.0, add the fermented material and sterile water at a weight ratio of 1:3.5 and mix well. If the pH value does not reach 9.0, continue fermentation for 3 days. Add ingredients ⑤ and ⑥ from the above-mentioned materials and mix well. Then, aerate and ferment for 10 days. After 10 days, when the pH value is 4, filter out the bacterial residue, take the bacterial liquid, add ingredient ⑧ from the above-mentioned materials and mix well. Static fermentation for 2 days. Then, add ingredient ⑦ from the above-mentioned materials and mix well. Static fermentation for 3 days. After 3 days, package the product in buckets to obtain the finished product.

[0509] The test data for the saline water after treatment in Examples 1-2 are as follows:

[0510] 1) No. 1 aquaculture base of Zhanyu Aquatic Products Company, Kezhou, Xinjiang Uygur Autonomous Region.

[0511] Water quality test record on May 28th

[0512] Saltwater inlet (#1) salinity: 11 parts per thousand

[0513] pH value: 8.9

[0514] Nitrite concentration: 0.12

[0515] Calcium ion concentration: 200 mg / L

[0516] Magnesium ion concentration: 61 mg / L

[0517] Ammonia content: 0

[0518] Potassium ion content: 261 mg / L

[0519] Total alkalinity content: 290 mg / L

[0520] Bicarbonate alkalinity: 110 mg / L.

[0521] Freshwater inlet (#2)

[0522] Salinity: 0 parts per thousand

[0523] pH value: 7.5

[0524] Nitrite concentration: 0.05

[0525] Calcium ion concentration: 100 mg / L

[0526] Magnesium ion concentration: 182 mg / L

[0527] Ammonia content: 0

[0528] Potassium ion content: 131 mg / L

[0529] Total alkalinity: 210 mg / L

[0530] Bicarbonate alkalinity: 190 mg / L.

[0531] Area A, Datang (#3)

[0532] Salinity: 10 parts per thousand

[0533] pH value: 8.3

[0534] Nitrite concentration: 0.10

[0535] Calcium ion concentration: 100 mg / L

[0536] Magnesium ion concentration: 300 mg / L

[0537] Ammonia content: 0

[0538] Potassium ion content: 148 mg / L

[0539] Total alkalinity content: 280 mg / L

[0540] Bicarbonate alkalinity: 180 mg / L.

[0541] Area B, Datang (#4)

[0542] Salinity: 11 parts per thousand

[0543] pH value: 8.5

[0544] Nitrite concentration: 0.10

[0545] Calcium ion concentration: 100 mg / L

[0546] Magnesium ion concentration: 300 mg / L

[0547] Ammonia content: 0

[0548] Potassium ion content: 155 mg / L

[0549] Total alkalinity content: 270 mg / L

[0550] Bicarbonate alkalinity: 170 mg / L.

[0551] Area C, Datang (#5)

[0552] Salinity: 10.5 parts per thousand

[0553] pH value: 8.5

[0554] Nitrite concentration: 0.12

[0555] Calcium ion concentration: 100 mg / L

[0556] Magnesium ion concentration: 337 mg / L

[0557] Ammonia content: 0

[0558] Potassium ion content: 155 mg / L

[0559] Total alkalinity content: 270 mg / L

[0560] Bicarbonate alkalinity: 170 mg / L.

[0561] 2) Zhanyu Aquatic Products Company Aquaculture Base No. 1, Kezhou, Xinjiang Uygur Autonomous Region.

[0562] Water quality test record on May 31st

[0563] Saltwater inlet (#1) salinity: 11 parts per thousand; pH value: 8.5

[0564] Nitrite concentration: 0.11

[0565] Calcium ion concentration: 200 mg / L

[0566] Magnesium ion concentration: 61 mg / L

[0567] Ammonia content: 0

[0568] Potassium ion content: 261 mg / L

[0569] Total alkalinity content: 290 mg / L

[0570] Bicarbonate alkalinity: 110 mg / L.

[0571] Freshwater inlet (#2)

[0572] Salinity: 0 parts per thousand

[0573] pH value: 7.8

[0574] Nitrite concentration: 0.05

[0575] Calcium ion concentration: 100 mg / L

[0576] Magnesium ion concentration: 182 mg / L

[0577] Ammonia content: 0

[0578] Potassium ion content: 131 mg / L

[0579] Total alkalinity: 210 mg / L

[0580] Bicarbonate alkalinity: 190 mg / L.

[0581] Area A, Datang (#3)

[0582] Salinity: 10 parts per thousand

[0583] pH value: 8.3

[0584] Nitrite concentration: 0.05

[0585] Calcium ion concentration: 100 mg / L

[0586] Magnesium ion concentration: 300 mg / L

[0587] Ammonia content: 0

[0588] Potassium ion content: 148 mg / L

[0589] Total alkalinity content: 280 mg / L

[0590] Bicarbonate alkalinity: 180 mg / L.

[0591] Area B, Datang (#4)

[0592] Salinity: 11 parts per thousand

[0593] pH value: 8.5

[0594] Nitrite concentration: 0.05

[0595] Calcium ion concentration: 100 mg / L

[0596] Magnesium ion concentration: 300 mg / L

[0597] Ammonia content: 0

[0598] Potassium ion content: 155 mg / L

[0599] Total alkalinity content: 270 mg / L

[0600] Bicarbonate alkalinity: 170 mg / L.

[0601] Area C, Datang (#5)

[0602] Salinity: 10.5 parts per thousand

[0603] pH value: 8.3

[0604] Nitrite concentration: 0.10

[0605] Calcium ion concentration: 100 mg / L

[0606] Magnesium ion concentration: 337 mg / L

[0607] Ammonia content: 0

[0608] Potassium ion content: 155 mg / L

[0609] Total alkalinity content: 270 mg / L

[0610] Bicarbonate alkalinity: 170 mg / L.

[0611] After successfully restoring the water source from Examples 1-2, the results of using it for aquaculture of various seafood were significant.

[0612] For example, at the aquaculture base of Zhanyu Company in Kizilsu Kyrgyz Autonomous Prefecture (Kizilsu for short), part of the Shishixian Aquaculture Group, located in Halajun Township, Artush City, situated in the vast Xiaoerkule Salt Lake, the aquaculture of seafood is now very successful. The following are examples of the results:

[0613] 1. The golden drum fish fry were stocked on July 10, 2024, and tested on August 2. They measured 11 cm in length and 100 g in weight. (See attached document for details) Figure 7 )

[0614] 2. The golden pomfret was stocked on June 13, 2024, and tested on August 2. It measured 13 cm in length and weighed 195 grams. (See details) Figure 8 )

[0615] 3. The seeding time for the pearl grouper was June 10, 2024; the testing time was August 2, 2024. The fish measured 20 cm in length and 400 g in weight. (See attached document for details) Figure 10 )

[0616] 4. Yellow-flagged catfish (Yellow-legged flounder) were stocked on June 13, 2024, and tested on August 2. They measured 15 cm in length and weighed 160 grams. (See attached document for details) Figure 6 )

[0617] 5. Oyster stocking time: July 1, 2024; testing time: August 2, 2024; body length: 48.4 mm; (see attached document for details) Figure 12 )

[0618] 6. The seedlings for the East China Sea pearl were introduced on June 1, 2024, and tested on August 2. The pearl's body length was 14.1 mm, and the pearl size was 0.69 mm. (See attached document for details) Figure 11 )

[0619] 7. The stocking time for sea mullet (horsehead mullet) was the 15th day of the first lunar month in 2024. Testing was conducted on August 2nd; the mullet measured 35 cm in length and 750 g in weight. (See attached document for details) Figure 9 )

[0620] 8. The large pearl grouper was stocked on May 1, 2024, and tested on August 2. It measured 30 cm in length and weighed 1003 g. (See attached document for details) Figure 13 )

[0621] 9. Mudskipper fry were stocked on June 25, 2024, and tested on August 2. They measured 14 cm in length and 150 g in weight. (See attached document for details) Figure 14 )

[0622] 10. Seaweed seedlings were planted on July 1, 2024, and tested on August 2. Salinity was 22‰. Growth and yield were good, with a yield of approximately 3 tons per mu per month. (See attached document for details) Figure 15 )

[0623] The main material cost of this invention is as follows: Two bottles each of the following three aquaculture-specific fish fertilizer products involved in this invention—Water Management Agent (Type A or Type B), Aquatic Organism Health Preservative (Type C), and Water Balance Agent (Type 1 or Type 2)—500 grams per bottle, with a selling price of 25 yuan per 500 grams for each product. (See the submitted supporting document: the applicant's product manual for details.)

[0624] Based on 10,000 cubic meters of water: Adding the three products more than once a week for three consecutive weeks will successfully restore the seawater. Each week, adding one of the three products costs two bottles (1000g) × 25 yuan = 50 yuan. A total of six bottles (3000g) of the three products costs 25 yuan each × 25 yuan = 150 yuan. Other materials, including machinery, heterotrophic photosynthetic bacteria solution, autotrophic photosynthetic bacteria solution, and yeast powder, cost 25 yuan each. The total cost is 225 yuan, calculated as one application per week × 3 weeks = 675 yuan.

[0625] Total cost: Restoring 10,000 cubic meters of water costs only 675 yuan.

[0626] The invention process of using microorganisms to reduce saline water in Xinjiang Uygur Autonomous Region to modern seawater:

[0627] The inventor noticed some shallow pools and ponds in the salt lake, some red and some purple, and speculated that they contained microorganisms. The inventor took several kilograms of samples from the red and purple saline water and analyzed them, discovering that the colored water contained organic matter and microorganisms. Microscopic observation confirmed the presence of a type of microorganism, a fungus. Wherever organic matter exists, microorganisms will exist. The southern region of Xinjiang Uyghur Autonomous Region was once part of the Mediterranean Sea hundreds of millions of years ago, but was isolated due to tectonic shifts. Over hundreds of millions of years, freshwater evaporated and disappeared, leaving behind various salts in the seawater. The chemical and physical structures of these salts changed with the continuous evaporation of seawater, and the salinity increased, making it unsuitable for modern fish. However, modern seawater changes daily, and the salinity and other organic matter also change, making it suitable for modern fish. The initial experiment involved reducing salinity for cultivation, but the results were initially unsatisfactory. The inventor then collected water samples from the salt lake. Recalling his past studies on the origins of ancient Earth life and combining this with other research, the inventor discovered three questions:

[0628] Firstly, the inventors discovered that the earliest life forms on Earth were archaea, specifically *Halobacterium*. The core chemical components for photosynthesis in these archaea are retinaldehyde and various microorganisms capable of utilizing green light. At that time, Earth lacked both oxygen and organic matter, yet they still survived, demonstrating remarkable resilience. Subsequently, *Halobacterium* and other light-utilizing microorganisms gradually converted inorganic matter into organic matter. The ancient Earth was anaerobic, and the ability of *Halobacterium* to survive in salt lakes today is a result of continuous evolution to adapt to an oxygen-rich environment. Therefore, the *Halobacterium* in salt lakes must differ from their ancestors.

[0629] Secondly, halophilic bacteria appear reddish-purple because they can utilize the most energy-rich green light in sunlight and can survive in extreme environments. This indicates that they were among the earliest organisms on Earth to utilize light energy. The halophilic bacteria that dominated the primitive oceans primarily absorbed green light, but not red or violet light. Later photosynthetic organisms, unable to utilize green light, evolved to absorb and utilize the red and violet light that halophilic bacteria did not absorb—these are the types of chlorophyll-producing microorganisms (cyanobacteria and algae).

[0630] Third, a series of changes that occurred on ancient Earth were attributed to the emergence of cyanobacteria and cyanobacteria. These organisms proliferated, converting inorganic matter and sunlight into organic matter. Organic matter accumulated in Earth's oceans, and subsequently, heterotrophic and autotrophic photosynthetic bacteria appeared. These microorganisms, capable of surviving in both anaerobic and aerobic environments, shared common biological characteristics: they all relied on sunlight and both inorganic and organic matter to participate in life activities.

[0631] The life activities of these microorganisms caused subtle changes to the ancient Earth. Cyanobacteria initially struggled to grow on the surface of ancient oceans because they relied on red and violet light and were afraid of green light. Over time, they gradually adapted from the ocean floor to the surface environment. However, halophilic bacteria required the ocean surface to grow because they primarily absorbed green light for their life activities. Therefore, the water on Earth at that time was purple. Cyanobacteria continuously changed themselves, adapting to the upper layers of the ancient ocean and multiplying rapidly, releasing large amounts of oxygen during their life activities. The accumulation of oxygen caused the halophilic bacteria to gradually die out. The cyanobacteria also migrated from the seabed to the middle and upper reaches of the ocean. This evolutionary process allowed them to multiply rapidly. The massive reproduction of cyanobacteria continuously released oxygen, causing the halophilic bacteria to slowly die.

[0632] The remains of dead halophilic bacteria were decomposed by heterotrophic and autotrophic photosynthetic bacteria. These bacteria transformed inorganic matter unsuitable for marine life on ancient Earth into organic matter; and then transformed organic matter unsuitable for seafood into inorganic matter suitable for marine life and many types of seafood, providing abundant nutrients for cyanobacteria. Cyanobacteria continuously absorbed these nutrients, allowing them to proliferate on Earth, and their life activities continuously supplied oxygen to the planet, creating the life forms we know today.

[0633] Inspired by the above three points, the inventor mixed saline-alkali soil, underground saline-alkali water, and salt lake water into three different specific gravities: one adjusted to 15‰, the second to 20‰, and the third to 30‰. These were then subjected to high-temperature inactivation. Due to limited resources at the time, only a rice cooker could be used to heat the mixture to 200 degrees Celsius. After high-temperature inactivation, tests showed that there were virtually no organisms in the water. The inactivated water samples were then placed in three containers, and these three water samples were placed in a transparent container with halophilic bacteria culture enriched from the salt lake. The air was removed, and the containers were then placed in sunlight. After about 10 days, the color of the inoculum changed from slightly reddish-purple to pink. After another 5 days, the color changed from pink to red. This color change is a characteristic of the microorganisms transforming from larvae to adults (this is within the inventor's knowledge). The inventor then traveled to other salt lakes for further investigation. Returning after five days, approximately 20 days, the color of the inoculum had become the same as that of the salt lake, and tests showed that the microorganisms in the containers had matured. The inventors believed that as long as there was life in the salt lake, inorganic matter could be assimilated into organic matter. Organic matter contains numerous components; bacteria and algae contain sugars, amino acids, calcium, magnesium, phosphorus, potassium, and many other elements. Through continuous accumulation and regeneration, this aligns with the scientific theory of matter transformation. Experiments showed that the substances in the sample water achieved the transformation data required by the inventors.

[0634] Then, the inventor brought the experimental water back to Qinzhou City. His experimental equipment in Qinzhou was relatively complete, and tests revealed that the data obtained from the transformation of the saline water by halophilic bacteria had an 87% similarity to that of modern seawater. The inventor was extremely excited and immediately called a friend to explain this progress. He then asked his friend to send another 1000 kg of water to Qinzhou City, mixing salt lake water, underground saline water, and water from drainage ditches used for cotton cultivation. After receiving the water samples, the inventor first analyzed them. He firmly believed that he could achieve the same transformation data that Earth had previously been able to achieve. Previously, Earth's transformation relied on natural processes, requiring continuous evolution and mutation of microbial species, which took a long time. Now, the inventor, by utilizing the biological characteristics of natural microorganisms, has greatly shortened the process of reducing ancient marine materials. The natural transformation process of ancient marine materials is inevitably less efficient than modern artificial transformation because the inventor artificially introduced halophilic bacteria, cyanobacteria, photosynthetic bacteria, and various beneficial bacteria. The transformation process of ancient Earth's oceans, which took hundreds of millions of years, can now be completed in just 20-30 days using the methods described above.

[0635] The specific steps for reducing and converting saline-alkali water are as follows:

[0636] First, *Halobacter* possesses the ability to convert salt into various substances such as ammonia, nitrite, and nitrate. *Halobacter* belongs to a class of bacteria that can survive in high-salt environments. Through metabolism, it converts inorganic salts and organic matter in salt lakes into nutrients it needs, releasing some metabolic products. *Halobacter* obtains energy through the conversion of organic or inorganic substances, while simultaneously converting salt into other substances. These substances help maintain cell osmotic pressure in high-salt environments, thus protecting cells from damage caused by high salinity. The substances excreted by *Halobacter* during its life activities mainly include carbon dioxide, water, ammonia, and acetic acid. Carbon dioxide is a product of cellular respiration, water is a product of metabolic reactions, ammonia is a product of protein metabolism, and acetic acid is a product of the metabolism of certain organic acids. Furthermore, these excretions vary with environmental changes; for example, under different salinity, temperature, and pH conditions, the metabolic products of *Halobacter* may differ. The bacterial cells of *Halobacter* themselves contain various substances such as proteins, nucleic acids, polysaccharides, and lipids. These substances are all basic building blocks of cells and are of great significance in maintaining cell structure and function. The cell composition of *Halobacter* is also affected by environmental factors. For example, in high-salt environments, *Halobacter* may synthesize special proteins and polysaccharides to adapt to this environment. This invention utilizes this characteristic of *Halobacter* to conduct preliminary work on the reduction and transformation of saline-alkali water, creating an excellent aquatic and material environment for the subsequent inoculation of heterotrophic photosynthetic bacteria, autotrophic photosynthetic bacteria, and yeast.

[0637] Secondly, the environment and substances formed by the saline water after the initial reduction and transformation by the aforementioned halophilic bacteria, due to their ancient material structure, are not suitable for the survival and growth of current marine animals. Therefore, the saline water in the pond must be inoculated with heterotrophic photosynthetic bacteria. Heterotrophic photosynthetic bacteria use light as energy and various organic matter as their main carbon source, assimilating various organic matter to form their own substances. In the water, they can absorb and assimilate a large number of fine molecular organic matter. This helps prevent other harmful bacteria from converting organic matter into harmful nitrite forms, playing a role in wastewater treatment and environmental purification. There are also autotrophic photosynthetic bacteria, which absorb inorganic matter as their own nutrition, converting and utilizing ammonia nitrogen, nitrite, and hydrogen sulfide in the water, effectively improving water quality, balancing pH, and accelerating the nutrient cycle in the water. Finally, there are yeasts, which can utilize nutrients in the fermentation substrate to synthesize their own proteins and B vitamins, converting various organic wastewaters into bacterial proteins, vitamins, various enzymes, and hormones.

[0638] The aforementioned bacteria can transform and utilize the transforming substances of halophilic bacteria, as well as their own metabolites, secretions, and other organic and inorganic substances, and synthesize their own bacterial matter. This process belongs to the primary circular production process of material transformation from nothing to something. This creates a favorable aquatic and material environment for the bacterial community introduced into the patented product: aquaculture-specific fish fertilizer (ZL200910137979.6) preparation.

[0639] Finally, the bacterial culture of the patented product, aquaculture-specific fish fertilizer (ZL200910137979.6), was introduced. The effective components of this bacterial culture are:

[0640] Thermophilic Clostridium thermocellum;

[0641] Note: This bacterium is suitable for high-temperature fermentation. It produces a polycellulase complex in the fermented material, which can dissolve various fibers and lignin. Its solution contains a small amount of ethanol.

[0642] Black-red spiral actinomycetes, Latin name: Actinomyces melanocyles; Rose-colored actinomycetes, Latin name: A. roseus; Fiber actinomycetes, Latin name: A. cellulose;

[0643] Note: These three types of bacteria have basically the same function and properties. They can decompose organic matter and produce extracellular enzymes to break down proteins, cellulose, lignin, and chitin. Therefore, this property can be used to deal with substances with chitinous cell walls. The chitin-degrading enzymes secreted by this bacterial group can dissolve various pathogenic hyphae.

[0644] Aspergillus oryzae;

[0645] Note: This bacterium can produce saccharifying mold, cellulose mold, and phytic acid mold in fermentation materials. It can degrade linear and branched inoculum powder, crude fiber, and phytic acid into various fine molecular and free substances, and degrade macromolecular proteins into peptone, polypeptides, and various amino acids.

[0646] Rhizopus, a fungus with the Latin name Rhizopus;

[0647] Note: This bacterium is a fermentation aid in molds, and its secretions can help catalyze decomposition during mold fermentation.

[0648] Trichoderma viride:

[0649] Note: This bacterium is a glucan glycoside hydrolysant (enzyme). It has extremely strong catalytic properties for converting cellulose and lignin into hydrolysates.

[0650] Yeast, its Latin name is Saccharomyces; white ground mold, its Latin name is:

[0651] Geotrichumcandidum;

[0652] Note: These two types of bacteria have basically the same function and properties. They can use the nutrients in the fermentation substrate to synthesize their own proteins and B vitamins, and convert various organic wastewaters into bacterial proteins, vitamins, various enzymes, hormones, etc.

[0653] Lactic acid bacteria, its Latin name is: LAB. Lactic acid bacteria;

[0654] Note: This bacterium can decompose carbohydrates in materials to form lactic acid, playing a balancing role in multi-strain fermentation.

[0655] heterologous lactic acid fermentation bacteria:

[0656] Note: This bacterium can secrete acetic acid, succinic acid, hydrogen, and carbon dioxide into the material during multi-microbial fermentation, providing nutrients for other microbial groups and playing a coordinating role in purifying the environment.

[0657] Photoautotrophic or chemoautotrophic photosynthetic bacteria

[0658] Description: This bacterium absorbs inorganic matter as its own nutrition, transforms and utilizes ammonia nitrogen, nitrite, and hydrogen sulfide in the water, effectively improves water quality, balances pH value, and accelerates the cycle of nutrients in the water.

[0659] Heterotrophic purple nitrogen-fixing photosynthetic bacteria

[0660] Note: This bacterium uses light as its energy source and various organic substances as its primary carbon source. It can assimilate various organic substances to form its own substances, and in water bodies, it can absorb and assimilate a large number of fine molecular organic substances. It is of great significance in preventing other harmful bacteria from transforming organic matter into harmful subspecies forms, and in wastewater treatment and environmental purification.

[0661] This microbial community effectively inhibits the reproduction of pathogenic bacteria in aquatic animals, solves pollution problems in aquaculture, enhances animal disease resistance, and maintains the ecological balance of aquatic bodies. In aquaculture, there is no need to add chemical fertilizers or chemicals to ponds. Simply add a small amount of river or seawater to the pond periodically, followed by the application of this microbial preparation. This will provide the necessary nutrients for the microorganisms in the pond, fostering and propagating abundant primary live feed and microbial species. A tight-knit circular ecological environment and food chain are formed in the water, rapidly degrading organic matter and harmful substances. Through the massive reproduction and metabolic products of the microorganisms in the product, organic matter in the water is rapidly and effectively degraded, and various harmful substances such as ammonia nitrogen, nitrite, sulfides, and phosphorus are decomposed. This microbial community can also reduce substances derived from saline water by halophilic bacteria, heterotrophic photosynthetic bacteria, autotrophic photosynthetic bacteria, and yeast, thus adapting them to an environment beneficial to phytoplankton and zooplankton. The restored saline-alkali water body, through the abundant reproduction and cyclical life activities of beneficial phytoplankton and zooplankton, brings calcium, magnesium, potassium, trace elements, and various organic substances to the water body, which are continuously accumulated. The structure of the accumulated substances in the water body also changes continuously, eventually reaching the water quality standard suitable for raising marine fish, shrimp, crabs, and shellfish.

[0662] The embodiments described above are merely illustrative of several implementations of the present invention, and while the descriptions are specific and detailed, they should not be construed as limiting the scope of the present invention. It should be noted that those skilled in the art can make various modifications and improvements without departing from the concept of the present invention, and these modifications and improvements all fall within the scope of protection of the present invention. Therefore, the scope of protection of this patent should be determined by the appended claims.

Claims

1. A method for using microorganisms to reduce saline water into modern seawater suitable for cultivating various seafood, characterized in that... Includes the following steps 1. Pretreatment of saline-alkali water Pump saline water with a salinity of 15-30 wt‰ into the aquaculture pond and turn on the air compressor to fully mix the air with the saline water, promoting the precipitation of some substances for subsequent treatment; control the pH of the saline water to 7.3-7.5 during the pretreatment process. II: Microbial Culture Purple halobacterium colonies that survive in coastal high-salt and alkaline environments and purple halobacterium colonies that survive in high-altitude salt lakes were selected. After multi-stage enrichment, the dominant halobacterium colonies of each species were selected and mixed to obtain a purple mixed halobacterium strain for later use. A mixture is prepared by mixing saline water from saline-alkali ground with a salinity of 10-20 wt‰, saline water from salt lakes with a salinity of 180-200 wt‰, and highly saline-alkali soil containing some organic matter. This mixture is then boiled at 200-210℃, sealed in a light-transmitting incubator, inoculated with a purple mixed halobacterium strain, and the air inside the incubator is removed. The mixture is then cultured under sunlight. When the substrate visually turns purple, it is ready for propagation and use. The pH is controlled at 8.3-8.5 during inoculation. 3: Reduction reaction The purple mixed halophilic bacteria cultured and propagated in step two were inoculated into the saline water of the breeding pond in step one, and the waterwheel equipment was turned on and stirred for two hours before being turned off. The reduction reaction is carried out in a still water environment. After 5-10 days, when the water appears reddish-brown or tea-brown, heterotrophic photosynthetic bacteria liquid, autotrophic photosynthetic bacteria liquid and yeast powder are added. After reacting together for 5-10 days, aquaculture-specific fish fertilizer is added. The oxygenation equipment is turned on 24 hours a day. The reaction is carried out for 3 days, and the pH is controlled to rise from 7.3-7.5 to 8.5-8.

8. IV: Water Quality Monitoring and Adjustment Real-time monitoring of various indicators of the saline-alkali water after reduction; adjustment of the inoculation amount of microorganisms in step three according to the biological characteristics of seafood; and detection of the saline-alkali water reduction and transformation process to ensure that the water quality meets the requirements of various seafood farming. Five: Farmed Seafood The restored saline water was used in a seafood farming experiment. First, fish fry, shrimp fry, crab fry, and shellfish fry were tested. If more than 90% of them survived after 48 hours, the restoration of seawater was confirmed to be successful. The aquaculture-specific fish fertilizer mentioned above is composed of the following raw materials mixed in the indicated weight ratios: 0.1–0.2 parts of inoculum of Clostridium thermophilum; 0.1–0.2 parts of inoculum of *Actinomyces rubrum*, *Actinomyces roseum*, and *Actinomyces fibrillaris*; 0.1–0.2 parts of seed powder of Aspergillus oryzae, Rhizopus, and Trichoderma viride, respectively; 18-20 parts of wheat bran mixture: Mix wheat bran and sterile water in a 1:1 weight ratio and set aside. Yeast and Geotrichum candida inoculum powder, 0.1–0.2 parts each; 0.5–0.6 parts of lactic acid bacteria and heterologous lactic acid fermentation inoculum, respectively; The inoculum of photoautotrophic or chemoautotrophic photosynthetic bacteria and heterotrophic purple nitrogen-fixing photosynthetic bacteria were 0.5–0.6 parts, respectively. 16-26 parts of bacterial nutrient solution: Mix 1-3 parts of potassium dihydrogen phosphate solution, 1-1.5 parts of calcium chloride, 1-1.5 parts of urea and 5-7 parts of sugar, and dissolve the resulting mixture with sterile water at a weight ratio of 1:2 for later use. The parts involved in the preparation of aquaculture-specific fish fertilizer are by weight.

2. The method for reducing saline water to modern seawater suitable for cultivating various seafood using microorganisms according to claim 1, characterized in that: In step one, 6677-6688 cubic meters of saline water with a salinity of 15-30wt‰ is pumped into the aquaculture pond. In step two, 500-510 grams of purple mixed halophilic bacteria were prepared for later use. In step two, 5000-5010 grams of saline-alkali water from saline-alkali ground with a salinity of 10-20 wt‰, 5000-5010 grams of saline-alkali water from salt lake with a salinity of 180-200 wt‰, and 500-510 grams of high-salt-alkali soil containing some organic matter are mixed to obtain a mixture. In step three, inoculate 10-20 grams of well-cultured and propagated mixed purple halobacteria per cubic meter of saline-alkali water; In step three, when the water appears reddish-brown or tea-brown, add 10-20 grams of heterotrophic photosynthetic bacteria solution with a concentration of 3-5 billion CFU / mL per cubic meter of water, add 10-20 grams of autotrophic photosynthetic bacteria solution with a concentration of 3-5 billion CFU / mL per cubic meter of water, and add 10-20 grams of yeast powder with a specification of 10 billion CFU / g per cubic meter of water. After reacting together for 5-10 days, add 30-31 grams of aquaculture-specific fish fertilizer per cubic meter of water.

3. A method for reducing saline water to modern seawater suitable for aquaculture of various seafood using microorganisms according to claim 1 or 2, characterized in that: In step three, aquaculture-specific fish fertilizer is introduced, and the oxygenation equipment is turned on 24 hours a day. After three days of reaction, beneficial mixed concentrated algae solution and beneficial zooplankton are introduced.

4. The method for reducing saline water to modern seawater suitable for cultivating various seafood using microorganisms according to claim 3, characterized in that: The beneficial mixed concentrated algae solution is added at a rate of 50 grams per cubic meter of water, including brown finger algae, marine green algae, and coral algae. The beneficial zooplankton solution is added at a rate of 50 grams per cubic meter of water, including live beneficial zooplankton such as water fleas and water fleas.

5. A method for reducing saline water to modern seawater suitable for aquaculture of various seafood using microorganisms according to claim 1 or 2, characterized in that: The pH for steps four and five is 8.5-8.

8.

6. A method for reducing saline water to modern seawater suitable for aquaculture of various seafood using microorganisms according to claim 1 or 2, characterized in that: Various seafood including golden pomfret, pearl grouper, red grouper, yellowfin seabream, oysters, mullet, mudskipper, and seaweed.

7. A method for reducing saline water to modern seawater suitable for aquaculture of various seafood using microorganisms according to claim 1 or 2, characterized in that: Various types of seafood do not require domestication and can be directly introduced into aquaculture.

8. A method for reducing saline water to modern seawater suitable for aquaculture of various seafood using microorganisms according to claim 1 or 2, characterized in that... The preparation of the aquaculture-specific fish fertilizer includes the following steps: (1) Material preparation: ① 0.1–0.2 parts of inoculum of Clostridium thermophilum; ② 0.1 to 0.2 parts of inoculum of black-red spiral actinomycetes, rose-colored actinomycetes, and fibrillary actinomycetes; ③ 0.1–0.2 parts of seed powder of Aspergillus oryzae, Rhizopus, and Trichoderma viride, respectively; ④ 18-20 parts of wheat bran mixture: Mix wheat bran and sterile water in a 1:1 weight ratio and set aside. ⑤ 0.1–0.2 parts each of yeast and Geotrichum candida inoculum powder; ⑥ 0.5–0.6 parts of lactic acid bacteria and heterologous lactic acid fermentation inoculum, respectively; ⑦ The inoculum of photoautotrophic or chemoautotrophic photosynthetic bacteria and heterotrophic purple nitrogen-fixing photosynthetic bacteria were 0.5–0.6 parts respectively; ⑧ 16-26 parts of bacterial nutrient solution: Mix 1-3 parts of potassium dihydrogen phosphate solution, 1-1.5 parts of calcium chloride, 1-1.5 parts of urea and 5-7 parts of sugar. Dissolve the mixture in sterile water at a weight ratio of 1:2 and set aside. The quantities involved in the material preparation are by weight. (2) Production: First, mix ingredients ①, ②, and ③ thoroughly. Then, add ingredient ④ and mix well. Ferment on an slant for 5 days, stirring once in the morning and once in the evening. After 5 days, take a sample and dissolve it in water. When the pH of the test solution reaches 9.0, mix the fermented material with sterile water at a weight ratio of 1:3.

5. If the pH does not reach 9.0, continue fermentation for 2-3 days. Add ingredients ⑤ and ⑥ thoroughly and mix well. Then, aerate and ferment for another 10 days. After 10 days, when the pH is 3-4, filter out the bacterial residue, take the bacterial liquid and mix it with ⑧ in the preparation material, and let it ferment statically for 2 days; then add ⑦ in the preparation material and mix well, and let it ferment statically for another 3 days; after 3 days, package it into buckets to obtain the finished product.

9. The application of a special aquaculture fertilizer in the modern seawater method described in any one of claims 1-8, which utilizes microorganisms to reduce saline water into seawater suitable for aquaculture of various seafood, characterized in that: The preparation of the aquaculture-specific fish fertilizer includes the following steps: (1) Material preparation: ① 0.1–0.2 parts of inoculum of Clostridium thermophilum; ② 0.1 to 0.2 parts of inoculum of black-red spiral actinomycetes, rose-colored actinomycetes, and fibrillary actinomycetes; ③ 0.1–0.2 parts of seed powder of Aspergillus oryzae, Rhizopus, and Trichoderma viride, respectively; ④ 18-20 parts of wheat bran mixture: Mix wheat bran and sterile water in a 1:1 weight ratio and set aside. ⑤ 0.1–0.2 parts each of yeast and Geotrichum candida inoculum powder; ⑥ 0.5–0.6 parts of lactic acid bacteria and heterologous lactic acid fermentation inoculum, respectively; ⑦ The inoculum of photoautotrophic or chemoautotrophic photosynthetic bacteria and heterotrophic purple nitrogen-fixing photosynthetic bacteria were 0.5–0.6 parts respectively; ⑧ 16-26 parts of bacterial nutrient solution: Mix 1-3 parts of potassium dihydrogen phosphate solution, 1-1.5 parts of calcium chloride, 1-1.5 parts of urea and 5-7 parts of sugar. Dissolve the mixture in sterile water at a weight ratio of 1:2 and set aside. The quantities involved in the material preparation are by weight. (2) Production: First, mix ingredients ①, ②, and ③ thoroughly. Then, add ingredient ④ and mix well. Ferment on an slant for 5 days, stirring once in the morning and once in the evening. After 5 days, take a sample and dissolve it in water. When the pH of the test solution reaches 9.0, mix the fermented material with sterile water at a weight ratio of 1:3.

5. If the pH does not reach 9.0, continue fermentation for 2-3 days. Add ingredients ⑤ and ⑥ thoroughly and mix well. Then, aerate and ferment for another 10 days. After 10 days, when the pH is 3-4, filter out the bacterial residue, take the bacterial liquid and mix it with ⑧ in the preparation material, and let it ferment statically for 2 days; then add ⑦ in the preparation material and mix well, and let it ferment statically for another 3 days; after 3 days, package it into buckets to obtain the finished product.

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